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| 1 | Rhizospheric microbial communities are driven by Panax ginseng at different growth stages and biocontrol bacteria alleviates replanting mortality显示文摘The cultivation of Panax plants is hindered by replanting problems, which may be caused by plantdriven changes in the soil microbial community. Inoculation with microbial antagonists may efficiently alleviate replanting issues. Through high-throughput sequencing, this study revealed that bacterial diversity decreased,whereas fungal diversity increased, in the rhizosphere soils of adult ginseng plants at the root growth stage under different ages. Few microbial community, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas,Sphingomonadaceae, and Zygomycota, were observed; the relative abundance of microorganisms, namely,Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota,increased in the soils of adult ginseng plants compared with those in the soils of 2-year-old seedlings. Bacillus subtilis 50-1, a microbial antagonist against the pathogenic Fusarium oxysporum, was isolated through a dual culture technique. These bacteria acted with a biocontrol efficacy of 67.8%. The ginseng death rate and Fusarium abundance decreased by 63.3% and 46.1%, respectively, after inoculation with B. subtilis 50-1. Data revealed that microecological degradation could result from ginseng-driven changes in rhizospheric microbial communities;these changes are associated with the different ages and developmental stages of ginseng plants. Biocontrol using microbial antagonists alleviated the replanting problem. | Linlin Dong Jiang Xu Lianjuan Zhang Ruiyang Cheng Guangfei Wei He Su Juan Yang Jun Qian Ran Xu Shilin Chen | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 27 |
| 2 | Integrated metabolomic and transcriptomic analyses revealed the distribution of saponins in Panax notoginseng显示文摘Panax notoginseng is famous for its important therapeutic effects. Saponins are bioactive compounds found in different parts and developmental stages of P. notoginseng plants. Thus, it is urgently to study saponins distribution in different parts and growth ages of P. notoginseng plants. In this study,potential biomarkers were found, and their chemical characteristic differences were revealed through metabolomic analysis. High-performance liquid chromatography data indicated the higher content of saponins(i.e., Rg1, Re, Rd, and Rb1) in the underground parts than that in the aerial parts. 20(S)-Protopanaxadiol saponins were mainly distributed in the aerial parts. Additionally, the total saponin content in the 3-year-old P. notoginseng plant(188.0 mg/g) was 1.4-fold higher than that in 2-year-old plant(130.5 mg/g). The transcriptomic analysis indicated the tissue-specific transcription expression of genes, namely, PnFPS, PnSS, PnSE1, PnSE2, and PnDS, which encoded critical synthases in saponin biosyntheses. These genes showed similar expression patterns among the parts of P. notoginseng plants.The expression levels of these genes in the flowers and leaves were 5.2 fold higher than that in the roots and fibrils. These results suggested that saponins might be actively synthesized in the aerial parts and transformed to the underground parts. This study provides insights into the chemical and genetic characteristics of P. notoginseng to facilitate the synthesis of its secondary metabolites and a scientific basis for appropriate collection and rational use of this plant. | Guangfei Wei Linlin Dong Juan Yang Lianjuan Zhang Jiang Xu Feng Yang Ruiyang Cheng Ran Xu Shilin Chen | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 13 |
| 3 | Treatment of coking wastewater by a novel electric assisted micro-electrolysis filter显示文摘A newly designed electric assisted micro-electrolysis filter(E-ME) was developed to investigate its degradation efficiency for coking wastewater and correlated characteristics. The performance of the E-ME system was compared with separate electrolysis(SE) and micro-electrolysis(ME) systems. The results showed a prominent synergistic effect on COD removal in E-ME systems. Gas chromatography/mass spectrometry(GC–MS) analysis confirmed that the applied electric field enhanced the degradation of phenolic compounds.Meanwhile, more biodegradable oxygen-bearing compounds were detected. SEM images of granular activated carbon(GAC) showed that inactivation and blocking were inhibited during the E-ME process. The effects of applied voltage and initial p H in E-ME systems were also studied. The best voltage value was 1 V, but synergistic effects existed even with lower applied voltage. E-ME systems exhibited some p H buffering capacity and attained the best efficiency in neutral media, which means that there is no need to adjust p H prior to or during the treatment process. Therefore, E-ME systems were confirmed as a promising technology for treatment of coking wastewater and other refractory wastewater. | Ruosong Xie Miaomiao Wu Guangfei Qu Ping Ning Yingying Cai Pei Lv | 2018 | Journal of Environmental Sciences2018，30，4： | 12 |
| 4 | Improved corrosion resistance and thermal stability of sintered Nd-Fe-B magnets with holmium substitution显示文摘The effects of Ho substitution for Nd on the microstructure, corrosion resistance and thermal stability of the Nd-Fe-B magnets were investigated. The(Nd,Ho)-O phase was formed with increasing Ho substitution. The results of potentiodynamic polarization and highly accelerated stress test show improved corrosion resistance with increasing Ho substitution. The optimum mass loss 0.29 mg/cm^2 is achieved.Moreover, the average temperature coefficients for remanence and coercivity in the range of 25-150℃are both closer to zero, indicating improved thermal stability. The mechanisms for the improved corrosion resistance and thermal stability are discussed in relation to the microstructure featuring the(Nd,Ho)-O phase. | Jinghui Di Shuai Guo Ling Chen Pengpeng Yi Guangfei Ding Kan Chen Ming Li Don Lee Aru Yan | 2018 | Journal of Rare Earths2018，36，8： | 11 |
| 5 | Location and PCR analysis of catabolic genes in a novel Streptomyces sp. DUT_AHX capable of degrading nitrobenzene显示文摘A novel strain of Streptomyces sp.DUT_AHX was isolated from sludge contaminated with nitrobenzene and identified on the basis of physiological and biochemical tests and 16S ribosomal DNA (rDNA) sequence analysis.The optimal degradation conditions were as follows:temperature 30℃,pH 7.0-8.0,shaking speed 150-180 r/min,and inocula 10%(V/V).The strain,which possessed a partial reductive pathway with the release of ammonia,was also able to grow on mineral salts basal (MSB) medium plates with 2-aminophenol, ph... | Al Haixin Zhou Jiti Lv Hong Wang Jing Guo Hanb Liu Guangfei Qu Yuanyuan | 2008 | Journal of Environmental Sciences2008，20，7： | 7 |
| 6 | Sirt3-MnSOD axis represses nicotine-induced mitochondrial oxidative stress and mtDNA damage in osteoblasts显示文摘 | Yong Li Chen Yu Guangsi Shen Guangfei Li Junkang Shen Youjia Xu Jianping Gong | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 6 |
| 7 | DOA Estimation Using Block Variational Sparse Bayesian Learning显示文摘In Direction-of-arrival(DOA) estimation,the real-valued sparse Bayesian algorithm degrades the estimation performance by decomposing the complex value into real and imaginary components and combining them independently. We directly use complex probability density functions to model the noise and complex-valued sparse direction weights. Based on the Multiple measurement vectors(MMV), block sparse structure for the direction weights is integrated into the variational Bayesian learning to provide accurate source direction estimates. The proposed algorithm can be used for arbitrary array geometries and does not need the prior information of the incident signal number. Simulation results demonstrate the better performance of the proposed method compared with the real-valued sparse Bayesian algorithm, the Orthogonal matching pursuit(OMP) and l1 norm based complexvalued methods. | HUANG Qinghua ZHANG Guangfei FANG Yong | 2017 | Chinese Journal of Electronics2017，26，4： | 6 |
| 8 | Removal of nitric oxide from simulated flue gas via denitrification in a hollow-fiber membrane bioreactor显示文摘A hollow-fiber membrane bioreactor(HMBR) was studied for its ability to treat nitric oxide(NO) from simulated flue gas. The HMBR was operated for 9 months and showed a maximum elimination capacity of 702 mg NO/(m2·day) with a removal efciency of 86%(gas residence time of 30 sec, inlet NO concentration of 2680 mg/m3, pH 8). Varying operation parameters were tested to determine the stability and response of the HMBR. Both the inlet NO concentration and gas residence time influenced the removal of NO in the HMBR. NO elimination capacity increased with an increase in inlet NO concentration or a shortening of gas residence time. Higher removal efciency of NO was obtained at a longer gas residence time or a lower inlet NO concentration. Microbial communities of the HMBR were sensitive to the variation in pH value and alkalescence corresponding to an optimum pH value of 8. In addition, NO elimination capacity and removal efciency were inversely proportional to the inlet oxygen concentration. Sulfur dioxide had no great influence on elimination capacity and removal efciency of NO. Product analysis was performed to study N2O and N2 production and confirmed that the majority of the microorganisms were denitrifying bacteria in the HMBR. Compared to other bioreactors treating NO, this study showed that the denitrifying HMBR was a good option for the removal of NO. | Xinyu Zhang Ruofei Jin Guangfei Liu Xiyang Dong Jiti Zhou Aijie Wang | 2013 | Journal of Environmental Sciences2013，25，11： | 5 |
| 9 | Near-Field Source Localization Using Spherical Microphone Arrays显示文摘A new method is proposed for joint range and bearing(azimuth and elevation) estimation of multiple near-field acoustic sources using observations collected by a spherical microphone array. First, Spherical Fourier transform(SFT) is used to construct the array signal model in the spherical harmonics domain to decouple range and bearing information. Then the relation among the spherical harmonics of three adjacent degrees is exploited to build the recursive relationship of the signal subspace. Using Eigenvalue decomposition(EVD), bearings are estimated based on the eigenvalues and simultaneously the steering matrix can be represented by the signal subspace.Finally, range is estimated using the energy ratios of the elements of the steering matrix in the spherical harmonics domain. The algorithm can avoid parameter pairing and multi-dimensional searching. It has lower computational complexity than that of the Multiple signal classification(MUSIC) method. The performance is evaluated by Monte-Carlo simulations and the estimation root meansquare errors are compared to the corresponding CramerRao bounds(CRBs) and those of MUSIC range estimates,which demonstrate the validity of the proposed algorithm. | HUANG Qinghua ZHANG Guangfei LIU Kai | 2016 | Chinese Journal of Electronics2016，25，1： | 4 |
| 10 | A synthetic criterion for early recognition of cutting chatter显示文摘Cutting chatter is a violent self-excited vibration between a tool and a workpiece.Its negative effects mainly include poor surface quality,inferior dimensional accuracy,disproportionate tool wear or tool breakage,and excessive noise.Therefore,early recognition and online suppression of chatter vibration are necessary.This paper proposes a novel synthetic criterion(SC)for early chatter recognition.The proposed SC integrates standard deviation(STD)and one-step autocorrelation function(OSAF).Moreover,this paper revised the fast algorithm of OSAF.We can quantitatively divide a chatter vibration signal into three stages,which are stable stage,transition stage and chatter stage according to the SC.Compared with STD,the SC can improve the reliability of chatter recognition and the threshold of SC is not sensitive to variable cutting conditions.This paper presents an original algorithm of SC and its fast algorithm in detail.The fast algorithm of SC in this paper improves the computation efficiency compared with the original algorithm of SC.To validate the effectiveness of the proposed SC,a series of milling experiments were conducted under different cutting conditions.In these experiments,the vibration signals were acquired by two accelerometers mounted on the spindle house.The experimental results showed that the proposed SC could effectively recognize chatter vibration at an early stage of chatter vibration,which saved valuable time for online chatter suppression. | JIA GuangFei WU Bo HU YouMin XIE FengYun LIU Ao | 2013 | Science China(Technological Sciences)2013，56，11： | 3 |
| 11 | Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness显示文摘Tonic signaling of chimeric antigen receptor(CAR),i.e.,the spontaneous CAR activation in the absence of tumor antigen stimulation,is considered to be a pivotal event controlling CAR-T efficacy.However,the molecular mechanism underlying the spontaneous CAR signals remains elusive.Here,we unveil that positively charged patches(PCPs)on the surface of the CAR antigenbinding domain mediate CAR clustering and result in CAR tonic signaling.For CARs with high tonic signaling(e.g.,GD2.CAR and CSPG4.CAR),reducing PCPs on CARs or boosting ionic strength in the culture medium during ex vivo CAR-T cell expansion minimizes spontaneous CAR activation and alleviates CAR-T cell exhaustion.In contrast,introducing PCPs into the CAR with weak tonic signaling,such as CD19.CAR,results in improved in vivo persistence and superior antitumor function.These results demonstrate that CAR tonic signaling is induced and maintained by PCP-mediated CAR clustering.Notably,the mutations we generated to alter the PCPs maintain the antigen-binding affinity and specificity of the CAR.Therefore,our findings suggest that the rational tuning of PCPs to optimize tonic signaling and in vivo fitness of CAR-T cells is a promising design strategy for the nextgeneration CAR. | Jian Chen Shizhen Qiu Wentao Li Kun Wang Yu Zhang Han Yang Baichuan Liu Guangfei Li Li Li Min Chen Junjie Lan Jiahua Niu Peijie He Lei Cheng Gaofeng Fan Xin Liu Xianmin Song Chenqi Xu Haitao Wu Haopeng Wang | 2023 | Cell Research2023，33，5： | 2 |
| 12 | Genetic dissection of husk number and length across multiple environments and fine-mapping of a major-effect QTL for husk number in maize(Zea mays L.)显示文摘Husk number(HN)and husk length(HL)influence the mechanical harvesting of maize grain.We investigated the genetic basis of HN and HL using a population of 204 recombinant inbred lines phenotypically evaluated in five environments.The two husk traits showed broad phenotypic variation and high heritability.Nine stable quantitative trait loci(QTL)were identified by single-environment mapping,comprising four QTL for HN and five for HL,and three QTL explained>10%of the phenotypic variation.Joint mapping revealed 22 additive QTL and 46 epistatic QTL.Both additive and epistatic(additive×additive)effects as well as a few large-effect QTL and some minor-effect QTL appeared to contribute to the genetic architecture of HN and HL.The QTL for HN located on chromosome 7,q HN7,which accounted for^20%of phenotypic variation,was detected in all five environments.q HN7 was fine-mapped to a 721.1 kb physical region based on the maize B73 Ref Gen_v3 genome assembly.Within this interval,four genes associated with plant growth and development were selected as candidate genes.The results will be useful for improvement of maize husk traits by molecular breeding and provide a basis for the cloning of q HN7. | Guangfei Zhou Yuxiang Mao Lin Xue Guoqing Chen Huhua Lu Mingliang Shi Zhenliang Zhang Xiaolan Huang Xudong Song Derong Hao | 2020 | The Crop Journal2020，8，6： | 2 |
| 13 | The protective role of glycine betaine in Lactobacillus plantarum ST-III against salt stress显示文摘 | Shanshan Zhao Qiuxiang Zhang Guangfei Hao Xiaoming Liu Jianxin Zhao Yongquan Chen Hao Zhang Wei Chen | 2014 | Food Control2014，，： | 1 |
| 14 | Quinone- mediated reduction o显示文摘 | WANG Xiu]uan LIU Guangfei ZHOU jiti | 2011 | Bioresource Technology2011，102，3： | 1 |
| 15 | Kinetic model for combined (alkaline+ultrasonic) sludge ultrasonic显示文摘 | LI Chunlin LIU Guangfei JIN Ruofei | 2010 | Bioresource Technology2010，101，6： | 1 |
| 16 | Acceleration of azo dye decolorization by using quinone reductase activity of azoreductase and quinone redox mediator 显示文摘 | LIU Guangfei ZHOU Jiti WANG Jing | 2009 | Bioresource Technology2009，100，27： | 1 |
| 17 | Moisture sorption and thermodynamic properties of Camellia oleifera seeds as influenced by oil content显示文摘Moisture sorption isotherms and thermodynamic properties of Camellia oleifera seeds as influenced by oil content were investigated.Moisture desorption and adsorption isotherms of Camellia oleifera seeds,kernels and shells from three varieties were determined using constant temperature and humidity chamber method at different temperatures(10°C,25°C,and 40°C)with water activity ranging from 0.20 to 0.90.Six selected mathematic models were employed to fit the experimental data.The Peleg model gave the best results for both seeds and kernels and Langmuir model was the best for shells.The difference of equilibrium moisture contents at the same water activities during desorption and adsorption indicated the occurrence of hysteresis of adsorption processes and the equilibrium moisture contents tended to decrease with the increasing oil content and temperature.The binding energy and average capacity per unit mass decreased with increasing temperature and oil content.The relationships between water activity and the logarithm of sorption activity showed the capillary porous body characteristics of the seeds. | Guangfei Zhu Qi Jin Yanhong Liu Yawen Lin Jun Wang Xingyi Li | 2021 | International Journal of Agricultural and Biological Engineering2021，14，1： | 1 |
| 18 | Sapphirine-Bearing Pelitic Granulite from Ailaoshan Orogen,West Yunnan,China: Metamorphic Conditions and Tectonic Setting显示文摘To reveal the petrological characteristics, metamorphic evolution histon and tectonic setting of the pelitic granulites from Ailaoshan Orogen, Uest Yunnan, China, a comprehensive study in mineral chemistry, petrogeochemistry and geochronology studies is presented in this paper. Two metamorphic stages of the granulites can be established:(1) the peak metamorphism recorded by the mineral assemblage of garnet, kyanite, K-feidspar and rutile, and the initial retrograde metamorphism shown by the mineral assemblage of garnet, sillimanite, sapphirine, spinel, K-feldspar, plagioclase and biotite;(2) the superim-posed metamorphism recorded by the mineral assemblage of biotite, muscovite, plagioclase, quartz and ilmenite. Zircon LA-ICP-MS U-Pb dating indicates that the protolith of the granulite was deposited after 337 Ma. The initial retrograde metamorphism occurred at P-T conditions of 8.6-12 kbar at 850-920℃ estimated by mineral assemblages, the low pressure limit of kyanite stability and GBPQ geothermobarometer in Indosinian (about 235 Ma), and the late superimposed metamorphism occurred at the P-T condition of 3.5-3.9 kbar at 572-576℃ estimated by GBPQ geothermobarometer since 33Ma. The first stage was related to the amalgamation of the South China and Indochina blocks during the Triassic, and the second stage was possibly related with the large scale sinistral slip-shearing since the Oligocene. It is inferred that the upper continental crust was suhducted/underthrusted to the lower continental crust (deeper than 30 km) and underwent granulite-facies metamorphism and then quickly exhumed to the middle-upper crust (10-12 km) and initial retrograde metamorphism occurred due to the collision of the Indochina and South China blocks during Indosinian, which was followed by superimposition of the second stage of metamorphism since the Oligocene. | Cheng Wei Yuhao Zhao Luhua Zhu Xuexiang Qi Zhihui Cai Xufeng Liu Guangfei Ma | 2019 | Journal of Earth Science2019，30，3： | 1 |
| 19 | Study on ferromagnetic shape memory alloy Ni-Mn-Ga films显示文摘 | Li Changsheng Sun Jingwen Sun Guangfei | 2007 | Surface & Coatings Technology2007，201，： | 1 |
| 20 | Three novel photoisomeric compounds of the 4-acyl pyrazolone derivants: Crystal structures and substituent effects on photo-isomerism in solid state显示文摘Three new photoisomeric compounds: 1,3-diphenyl-4-benzal-5-pyrazolone 4-methylthiosemicarbazone (DPBP-MTSC), 1,3-diphenyl-4-(4′-methylbenzal)-5-pyrazolone 4-methylthiosemicarbazone (DP4MBP- MTSC), and 1,3-diphenyl-4-(4′-bromobenzal)-5-pyrazolone 4-methylthiosemicarbazone (DP4BrBP-MTSC) were synthesized by direct condensation of pyrazolones and 4-methylthiosemicarbazone. Their struc- tures were confirmed using 1H NMR, IR, elemental analyses, and X-ray crystallographic analyses. The photoisomeric properties in the solid state were studied under UV light irradiation and the photo- isomerization phenomena were interpreted by the double proton-transfer mechanism. Moreover, the effects of different substituent groups at the 4-position of the benzal in the three compounds on the photoisomeric properties were discussed. | WANG JunHua LIU Lang LIU GuangFei GUO JiXi JIA DianZeng | 2008 | Science China Chemistry2008，51，7： | 1 |
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