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| 1 | CULTURAL KNOWLEDGE IS NEEDED IN SECOND(FOREIGN)LANGUAGE TEACHING AND LEARNING IN CHINA显示文摘This article tackles the problem of cultural interferences in foreign language teaching andlearning for Chinese learners of English.It also calls for more attention from teachers,textbook com-pilers and students themselves. | Yan Guoquan Guangxi Baise Agricutural School | 1999 | Chinese Journal of Applied Linguistics1999，22，2： | 56 |
| 2 | Nascent Proteome and Glycoproteome Reveal the Inhibition Role of ALG1 in Hepatocellular Carcinoma Cell Migration显示文摘Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis. | Xinyi Cao Yuyin Shao Peiyi Meng Zhao Cao Guoquan Yan Jun Yao Xinwen Zhou Chao Liu Lei Zhang Hong Shu Haojie Lu | 2022 | Phenomics2022，2，4： | 2 |
| 3 | Cationic poly(?-caprolactone) surface functionalized mesoporous silica nanoparticles and their application in drug delivery显示文摘 | Yan Zhang Zhaojun Wang Weimin Zhou Guoquan Min Meidong Lang | 2013 | Applied Surface Science2013，，： | 1 |
| 4 | A functional polymorphism in MIR 196 A 2 is associated with risk and prognosis of gastric cancer显示文摘 | Shizhi Wang Guoquan Tao Dongmei Wu Haixia Zhu Yan Gao Yongfei Tan Meilin Wang Weida Gong Yan Zhou Jianwei Zhou Zhengdong Zhang | 2013 | Mol Carcinog2013，，1： | 1 |
| 5 | Effect of hydrogen on ductility of high strength 3Ni–Cr–Mo–V steels显示文摘 | Yan Liu Maoqiu Wang Guoquan Liu | 2014 | Materials Science & Engineering A2014，，： | 1 |
| 6 | New lysine-acetylated proteins screened by immunoaffinity and liquid chromatography-mass spectrometry显示文摘The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we report for the first time the proteomic screening of lysine-acetylated proteins from a mouse liver tissue,by a new approach of immunoaffinity purification of lysine-acetylated peptides combined with nano-HPLC/MS/MS analysis.We have found 20 lysine-acetylated proteins with 21 lysine-acetylated sites,among which 12 lysine-acetylated proteins and 16 lysine-acetylated sites have never been reported before.Notably,three acetyltransferases harboring in mitochondrion are newly discovered acetyltransferases responsible for the acetylation of nonhistone proteins.We have explored the significant patterns of residue preference by the hierarchical clustering analysis of amino acid residues surrounding acetylation sites,which could be helpful to the prediction of new sites of lysine acetylation.Our findings provide more candidates for studying the important roles played by acetylation in diverse cellular pathways and related human diseases. | ZHANG Bing ZHAO Chao LUO KaiXuan YAN GuoQuan YAO Jun WANG YingYin LU HaoJie FAN HuiZhi YANG PengYuan | 2010 | Science China Chemistry2010，53，1： | 1 |
| 7 | Response of peptide intensity to concentration in ESI–MS-based proteome显示文摘High-throughput quantification with label-free methods has received considerable attention in electrospray ionization(ESI)-mass spectrometry(MS),but the manner by which MS signals respond to peptide concentration remains unclear in proteomics.We developed a new mathematical formula to describe the intrinsic log-log relationship between the MS intensity response and peptide concentration in an analytical ESI process.Experimental results showed that the calibration curve is fairly fit to the log-log formula with a linear dynamic range of approximate four to five orders of magnitude.However,we found that the ionization of analytical peptides can be severely suppressed by coexisting matrix peptides,such that the calibration curve can be poorly leveled off on both ends.Our study suggests that the interferences from coexisting matrix peptides should be reduced in the ESI process to use the log-log calibration curve successfully for the high-throughput quantification. | LU WenYuan YIN XueFei LIU XiaoHui YAN GuoQuan YANG PengYuan | 2014 | Science China Chemistry2014，57，5： | 1 |
| 8 | Developing a strong anion exchange/RP (SAX/RP) 2D LC system for high‐abundance proteins depletion in human plasma显示文摘 | Shaochun Zhu Xueyang Zhang Mingxia Gao Guangfeng Hong Guoquan Yan Xiangmin Zhang | 2012 | Proteomics (-)2012，，23： | 1 |
| 9 | VanM, a New Glycoptide Resistance Gene Cluster Found in Enter- ococcus faecium 显示文摘 | Xiaogang Xu Dongfang Lin Guoquan Yan | 2010 | Antimicrobial Agents and Chemo- therapy2010，54，11： | 1 |
| 10 | Unbalanced expression of VEGF and PEDF in ischemia-induced retinal neovascularization显示文摘 | Guoquan Gao Yan Li Jian-xing Ma | 2001 | FEBS Lett2001，489，： | 1 |
| 11 | Novel high-PSRR high-order curvature-compensated bandgap voltage reference显示文摘This paper proposes a novel high-power supply rejection ratio(high-PSRR) high-order curvature-compensated CMOS bandgap voltage reference(BGR) in SMIC 0.18 μm CMOS process. Three kinds of current are added to a conventional BGR in order to improve the temperature drift within wider temperature range, which include a piecewise-curvaturecorrected current in high temperature range, a piecewise-curvature-corrected current in low temperature range and a proportional-to-absolute-temperature T^(1.5) current. The high-PSRR characteristic of the proposed BGR is achieved by adopting the technique of pre-regulator. Simulation results shows that the temperature coefficient of the proposed BGR with pre-regulator is 8.42x10^(-6)′ /℃ from - 55 ℃ to 125 ℃ with a 1.8 V power supply voltage. The proposed BGR with pre-regulator achieves PSRR of - 123.51 dB, - 123.52 dB, - 88.5 dB and - 50.23 dB at 1 Hz, 100 Hz, 100 kHz and 1 MHz respectively. | Zhou Qianneng Yan Kai Lin Jinzhao Pang Yu Li Guoquan Luo Wei | 2016 | The Journal of China Universities of Posts and Telecommunications2016，23，2： | 1 |
| 12 | An Improved Non-Parametric Method for Multiple Moving Objects Detection in the Markov Random Field显示文摘Detecting moving objects in the stationary background is an important problem in visual surveillance systems.However,the traditional background subtraction method fails when the background is not completely stationary and involves certain dynamic changes.In this paper,according to the basic steps of the background subtraction method,a novel non-parametric moving object detection method is proposed based on an improved ant colony algorithm by using the Markov random field.Concretely,the contributions are as follows:1)A new nonparametric strategy is utilized to model the background,based on an improved kernel density estimation;this approach uses an adaptive bandwidth,and the fused features combine the colours,gradients and positions.2)A Markov random field method based on this adaptive background model via the constraint of the spatial context is proposed to extract objects.3)The posterior function is maximized efficiently by using an improved ant colony system algorithm.Extensive experiments show that the proposed method demonstrates a better performance than many existing state-of-the-art methods. | Qin Wan Xiaolin Zhu Yueping Xiao Jine Yan Guoquan Chen Mingui Sun | 2020 | Computer Modeling in Engineering & Sciences2020，，7： | 1 |
| 13 | Near-infrared fluorescence imaging of CD13 receptor expression using a novel Cy5.5-labeled dimeric NGR peptide显示文摘 | Guoquan Li Yan Xing Jing Wang Peter S. Conti Kai Chen | 2014 | Amino Acids2014，，6： | 1 |
| 14 | Effect of hydrogen on ductility of high strength 3Ni–Cr–Mo–V steels显示文摘 | Yan Liu Maoqiu Wang Guoquan Liu | 2014 | Materials Science & Engineering A2014，，： | 1 |
| 15 | Proteomics analysis provides novel biomarkers and therapeutic target candidates in the treatment of the Huang-Pu-Tong-Qiao formula in an AD rat mode显示文摘Background:Huang-Pu-Tong-Qiao formula(HPTQ),a traditional Chinese herbal formula,has a variety of pharmacological effects.It has been used to treat Alzheimer’s disease(AD)for decades.This study aimed to screen differentially expressed proteins in the hippocampus of AD model rats treated with HPTQ.Proteomic studies of the effects of HPTQ on AD are key to understanding the therapeutic mechanisms of HPTQ and identifying potential therapeutic targets.Methods:We hence used the isobaric tags for relative and absolute quantification(ITRAQ)approach to investigate the differentially expressed proteins in the hippocampus of AD model rats before and after HPTQ administration and to identify the potential therapeutic target proteins of HPTQ.In this study,the learning and memory abilities of AD rats were examined by the Morris water maze test.After HPTQ administration,the differentially expressed proteins in the hippocampus of AD rats were quantified and analyzed in silico.Furthermore,western blotting was used to verify the expression of related proteins.Results:The Morris water maze results showed that HPTQ could improve the learning and memory ability of AD model rats.The proteomics analysis results showed that 57 proteins were differentially expressed,of which 35 were up-regulated and 22 were down-regulated.Bioinformatics analysis indicated that proteins with altered expression after HPTQ treatment were involved in several biological processes that have the potential to exert neuroprotective effects.These included promoting the translation of ribosomes,improving the deposition of amyloid-beta(Aβ),regulating autophagy,regulating neuronal synaptic function and plasticity,and alleviating oxidative stress.Conclusion:In conclusion,we identified several potential therapeutic target proteins and related mechanistic pathways of HPTQ in the treatment of AD,laying the foundation for further investigation of the therapeutic effects of HPTQ. | QIAN CHEN XIN LEI GUANHUA HU YAN WANG ZHENGQING FANG GUOQUAN WANG HANG SONG SHU YE BIAO CAI | 2023 | BIOCELL2023，47，6： | 0 |
| 16 | Physiological and Biochemical Characteristics and Response Patterns of Salinity Stress Responsive Genes (SSRGs) in Wild Quinoa (Chenopodium quinoa L.)显示文摘Cultivating salt-tolerant crops is a feasible way to effectively utilize saline-alkali land and solve the problem of underutilization of saline soils.Quinoa,a protein-comprehensive cereal in the plant kingdom,is an exceptional crop in terms of salt stress tolerance level.It seems an excellent model for the exploration of salt-tolerance mechanisms and cultivation of salt-tolerant germplasms.In this study,the seeds and seedlings of the quinoa cultivar Shelly were treated with different concentrations of NaCl solution.The physiological,biochemical characteristics and agronomic traits were investigated,and the response patterns of three salt stress-responsive genes(SSRGs)in quinoa were determined by real-time PCR.The optimum level of stress tolerance of quinoa cultivar Shelly was found in the range of 250–350 mM concentration of NaCl.Salt stress significantly induced expression of superoxide dismutase(SOD),peroxidase(POD),and particularly betaine aldehyde dehydrogenase(BADH).BADH was discovered to be more sensitive to salt stress and played an important role in the salt stress tolerance of quinoa seedlings,particularly at high NaCl concentrations,as it displayed upregulation until 24 h under 100 mM salt treatment.Moreover,it showed upregulation until 12 h under 250 mM salt stress.Taken together,these results suggest that BADH played an essential role in the salt-tolerance mechanism of quinoa.Based on the expression level and prompt response induced by NaCl,we suggest that the BADH can be considered as a molecular marker for screening salt-tolerant quinoa germplasm at the early stages of crop development.Salt treatment at different plant ontogeny or at different concentrations had a significant impact on quinoa growth.Therefore,an appropriate treatment approach needs to be chosen rationally in the process of screening salt-tolerant quinoa germplasm,which is useful to the utilization of saline soils.Our study provides a fundamental information to deepen knowledge of the salt tolerance mechanism of quinoa for the development of salt-tolerant germplasm in crop breeding programs. | Yurong Jiang Muhammad Yasir Yuefen Cao Lejia Hu Tongli Yan Shuijin Zhu Guoquan Lu | 2023 | Phyton-International Journal of Experimental Botany2023，92，2： | 0 |
| 17 | 大脑新皮层中突触前Cadherin/Catenin/p140Cap复合物对稳定树突棘与功能性突触起关键作用显示文摘文章简介功能性突触的形成依赖于突触前神经递质释放机器和突触后神经递质受体和骨架的协同参与。细胞粘附分子作为跨突触的桥梁,在突触前后的神经元沟通中起到纽带作用。 | Min-Yin Li Wan-Ying Miao Qiu-Zi Wu Shun-Ji He Guoquan Yan Yanrui Yang Jia-Jia Liu M.Mark Taketo 于翔 | 2018 | 科学新闻2018，0，4： | 0 |
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