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| 1 | A new bone repair scaffold combined with chitosan/ hydroxyapatite and sustained releasing icariin显示文摘Icariin, a plant-derived flavonol glycoside, has been proved as an osteoinductive agent for bone tissue engineering. A new bone repair scaffold was generated by thorough mixing of icariin and chitosan/ hydroxyapatite (icariin-CS/HA) using freeze-drying technigue. Characteristics of morphology, mechanical properties, biocompatibility, drug release behavior and bone repair abilities in vivo were evaluated. The results show that drug loading process of icariin did not affect physical structure of CS/HA composite significantly but decreased mechanical properies of CS/HA composite, which happened with a high dosage; icariin-CS/HA had favorable cell compatibility and promoted osteogenic differentiation of hBMSCs; the controlled release of icariin was satisfactory and the release retained after 90 d in vitro. In addition, icariin-CS/HA scaffolds had favorable osteoconduction and osteoinduction in vivo, and could fill bone defect sites and stimulate newborn bone tissues formation at early stage. On the basis of these data, icariin-CS/HA is believed to be an optical bone repair scaffold for tissue engineering. | WU Tao NAN KaiHui CHEN JingDi JIN Dan JIANG Shan ZHAO PeiRan XU JunChang DU Hao ZHANG XiaoQiang LI JianWei PEI GuoXian | 2009 | Chinese Science Bulletin2009，54，17： | 14 |
| 2 | Cardiac autonomic nerve fiber regeneration in chronic heart failure Do Akt gene-transduced mesenchymal stem cells promote repair?显示文摘BACKGROUND: Transplantation of Akt-over-expressing mesenchymal stem cells (Akt-MSCs) has been shown to repair infarcted myocardium and improve cardiac function. However, little is known about the therapeutic effects of Akt-MSCs on cardiac autonomic neuropathy in chronic heart failure (CHF). OBJECTIVE: The present study used adriamycin-induced CHF rat models to observe the effect of Akt-MSCs on cardiac autonomic nervous regeneration and the factors mediating this effect. DESIGN, TIME AND SETTING: A randomized, controlled animal experiment was performed at the Central Laboratory of Basic Medical College, China Medical University, between September 2008 and April 2009. MATERIALS: Rabbit anti-choline acetyltransferase (ChAT), growth associated protein-43 (GAP-43), synaptophysin (SYN) polyclonal antibodies and the secondary antibody (goat anti-rabbit IgG) were purchased from Boster, China. Cat-A-Kit assay system was provided by Amersham, USA. METHODS: (1) Adult rat MSCs were isolated and cultured for the preparation of Akt-MSCs. (2) Forty male Wistar rats were intramyocardially administered adriamycin at 2 mg/kg over 3 days for a total of five times and once a week for additional five times thereafter to establish CHF models. At 2 weeks after final adriamycin treatment, 34 successful CHF rat models were randomized to three groups: Akt-MSCs (n = 11), simple MSCs (s-MSCs, n =11), and control (n = 12). Each group was intravenously administered Akt-MSCs (2×106 cells in 100 μL PBS), s-MSCs (2×106 cells in 100 μL PBS) or equal volume of phosphate buffered saline, once a day for a total of three times. MAIN OUTCOME MEASURES: At 4 weeks after final adriamycin treatment, myocardial norepi- nephrine (NE) content was detected using a Cat-A-Kit assay system. Myocardial ChAT, SYN and GAP-43 were performed by immunohistochemistry and Western blot analysis. Prior to, 2 and 4 weeks after adriamycin treatment, echocardiographic examination was performed and left ventricular ejection fraction (LVEF) was determined. RESULTS: Myocardial NE content, as well as SYN-positive and GAP-43-positive nerve fiber density and expression, and LVEF, was the greatest in the Akt-MSCs group, followed by the s-MSCs group, and lastly the control group (P < 0.05 or P < 0.01). ChAT expression was similar between Akt-MSCs and s-MSCs groups, but it was higher compared with the control group (P < 0.05). NE contents were negatively correlated to LVEF (r = -0.64, P = 0.015). CONCLUSION: Transplantation of MSCs, in particular Akt-MSCs, promotes cardiac nervous regeneration in failing heart, which might be mediated by GAP-43. | Hongliang Kong Zhanquan Li Shumei Zhao Li Zhu Yingjun Zhao Weiwei Zhang GuipingXu Wenjun Hao Huijun Li Guoxian Qi | 2010 | Neural Regeneration Research2010，5，1： | 13 |
| 3 | EFFECT OF EQUAL-CHANNEL ANGULAR PRESSING ON STRUCTURE OF Al ALLOY 2024显示文摘EFFECTOFEQUALCHANNELANGULARPRESSINGONSTRUCTUREOFAlALLOY2024①LiuZuyan,LiangGuoxian,WangErde,WangZhongrenSchoolofMaterialsScie... | Liu, Zuyan Liang, Guoxian Wang, Erde Wang, Zhongren | 1997 | 中国有色金属学会会刊:英文版1997，7，2： | 12 |
| 4 | Cell multiplication, apoptosis and p-Akt protein expression of bone mesenchymal stem cells of rat under hypoxia environment显示文摘Objective: To elucidate whether cell multiplication, apoptosis, glucose intake and p-Akt protein expression of bone Mesenchy-mal Stem Cells(MSCs) of rats is influenced by a hypoxic environment ex vivo. Methods: Passage 3 of bone marrow MSCs takenfrom Wistar rats, were cultured in a culturing chamber with 94%N2, 1%O2, 5%CO2 at 37℃. At different hypoxia time points, 0, 0.5,1, 4 and 8 h, glucose uptake was assayed by using radiation isotope 3H-G, Apoptotic Rate(AR) and dead rate(DR) were analyzedby flow cytometry(FCM) after Annexin V/PI staining, cell multiplication(by MTT methods) and p-Akt protein by immunocyto-chemistry and western blot. Results: Assay for CD29+, CD44+, CD71+, CD34- , Tn T+(after 5-azacytidine agent inducing) andALP+(after bone differentiation agent inducing) suggested these bone-derived cells were MSCs. The 3H-G intaking ratio(CPM/flask value: 157 ±11, 110 ±11, 107 ±13, 103 ±10, 100 ±9 and 98 ±10) of MSCs at different hypoxia time points, significantlydecreased compared to that of normoxia(P < 0.01) and tended to descend slowly with hypoxia time duration, for which there wasno statistical significance(P > 0.05). The AR(0.09 ±2.03%, 12.9 ±1.72%, 13.7 ±2.26%, 13.8 ±3.01%, 14.1 ±2.78% and 14.7 ±4.01% at 0, 0.5, 1, 4 and 8 h, respectively, P < 0.01) and DR (0.04 ±1.79%, 0.93 ±1.85%, 3.11 ±2.14%, 4.09 ±2.36%, 4.72 ±2.05% and 4.91 ±3.72% at 0, 0.5, 1, 4 and 8 h, respectively, P < 0.05) at different hypoxia time points significantly increasedcompared to those time in normoxia; The AR further went up with time (P < 0.05), however there was no statistical significance(P > 0.05) for the DR. Optical absorption value of MTT methods at different hypoxia time points significantly decreased com-pared to those with a corresponding normoxia time (P < 0.01) and degraded with time (in an hypoxic environment - P < 0.01).IOD of p-Akt protein of MSCs at different hypoxia time points significantly increased (0.367 ±0.031, 0.556 ±0.023, 0.579 ±0.013, 0.660 ±0.024, 0.685 ±0.039 and 0.685 ±0.011, respectively) compared to their equivalents in normoxia (P<0.05), how-ever, there was no statistical significance (P > 0.05) for different hypoxia time points. Hypoxia may result in ultramicrostructurechanges, such as defluvium of Microvilli, apoptotic body, 'margination'and so on and are further aggravated with hypoxia timestretching. Conclusion: Hypoxia may lead to a depression of MSCs intaking glucose, creep of cell multiplication, upregulation ofp-Akt protein and apoptosis of MSCs ex vivo. | Hongliang Kong Ningning Liu Xin Huo Bo Wang Haipeng Zhang Mingyu Gao Guoxian Qi | 2007 | Journal of Nanjing Medical University2007，21，4： | 10 |
| 5 | Specific targeting of nasopharyngeal carcinoma cell line CNE1 by C225-conjugated ultrasmall superparamagnetic iron oxide particles with magnetic resonance imaging显示文摘临床的目标体积(CTV ) 的一个精确定义为在鼻咽的癌(NPC ) 的放射疗法的应用程序是必要的处理。指向对比代理人(C225-USPIO ) 的新奇表皮的生长因素受体(EGFR ) 被结合 ultrasmall superparamagnetic 设计有 cetuximab (C225 ) 的氧化铁(USPIO ) nanoparticles, non-invasively 定义肿瘤的 CTV。到 NPC 房间线 CNE1 的 C225-USPIO 的 immunobinding 活动被流动 cytometry 和 immunofluorescence 证实。在 CNE1 房间的 C225-USPIO 的时间依赖者累积用普鲁士的蓝染色被评估。指向的成为主观和 C225-USPIO 的 subcellular 本地化被传播电子显微镜证实。结果显示了明确地在 CNE1 房间的表面上绑在 EGFR 的那 C225-USPIO 并且被收起进房间。由 CNE1 房间的 C225-USPIO 的举起与时间显著地增加了,当时与人的 IgG-USPIO 相比。另外, 4.7 T 磁性的回声成像(MRI ) 随着时间的过去表明 C225-USPIO 有一个能力在 CNE1 房间积累,与在 MRI T2 加权的信号紧张的结果的显著减少。这些调查结果当一个 MRI 对比代理人和罐头被采用 non-invasively 与 EGFR overexpression 检测早阶段的 NPC,暗示 C225-USPIO 有潜力。这为与混合物在 vivo 实验开始提供足够的理论基础。 | Dongbo Liu Chunli Chen Guangyuan Hu Qi Mei Hong Qiu Guoxian Long Guoqing Hu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，4： | 10 |
| 6 | Nine-month angiographic and two-year clinical follow-up of polymer-free sirolimus-eluting stent versus durable-polymer sirolimus-eluting stent for coronary artery disease: the Nano randomized trial显示文摘 | Zhang Yaojun Chen Fang Takashi Muramatsu Xu Bo Li Zhanquan Ge Junbo He Qing Yang Zhijian Li Shumei Wang Lefeng Wang Haichang He Ben Li Kang Qi Guoxian Li Tianchang Zeng Hesong Peng Jianjun Jiang Tieming Zeng Qiutang Zhu Jianhua Fu Guosheng Christos V. Bourantas Patrick W. Serruys Huo Yong | 2014 | Chinese Medical Journal2014，，11： | 8 |
| 7 | Progressive loss of striatal dopamine terminals in MPTP-induced acute parkinsonism in cynomolgus monkeys using vesicular monoamine transporter type 2 PET imaging([^(18)F]AV-133)显示文摘The 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine(MPTP)-induced parkinsonism model, particularly in non-human primates, remains the gold-standard for studying the pathogenesis and assessing novel therapies for Parkinson's disease. However, whether the loss of dopaminergic neurons in this model is progressive remains controversial, mostly due to the lack of objective in vivo assessment of changes in the integrity of these neurons. In the present study, parkinsonism was induced in cynomolgus monkeys by intravenous administration of MPTP(0.2 mg/kg) for 15 days; stable parkinsonism developed over 90 days, when the symptoms were stable. Noninvasive positron emission tomographic neuroimaging of vesicular monoamine transporter 2 with 9-[18F]fluoropropyl-(+)-dihydrotetrabenazine([18F]AV-133) was used before, and 15 and 90 days after the beginning of acute MPTP treatment. The imaging showed evident progressive loss of striatal uptake of [18F]AV-133. The dopaminergic denervation severity had a significant linear correlation with the clinical rating scores and the bradykinesia subscores. Thesefindings demonstrated that [18F]AV-133 PET imaging is a useful tool to noninvasively evaluate the evolution of monoaminergic terminal loss in a monkey model of MPTP-induced parkinsonism. | Yajing Liu Feng Yue Rongping Tang Guoxian Tao Xiaomei Pan Lin Zhu Hank F.Kung Piu Chan | 2014 | Neuroscience Bulletin2014，30，3： | 6 |
| 8 | Small mammal community succession on the beach of Dongting Lake,China after the Three Gorges Project显示文摘Although the Three Gorges Project(TGP)may have affected the population structure and distribution of plant and animal communities,few studies have analyzed the effect of this project on small mammal communities.Therefore,the present paper compares the small mammal communities inhabiting the beaches of Dongting Lake using field investigations spanning a 20-year period,both before and after the TGP was implemented.Snap traps were used throughout the census.The results indicate that the TGP caused major changes to the structure of the small mammal community at a lake downstream of the dam.First,species abundance on the beaches increased after the project commenced.The striped field mouse(Apodemus agrarius)and the Norway rat(Rattus norvegicus),which rarely inhabited the beach before the TGP,became abundant(with marked population growth)once water was impounded by the Three Gorges Reservoir.Second,dominant species concentration indices exhibited a stepwise decline,indicating that the community structure changed from a single dominant species to a more diverse species mix after TGP implementation.Third,the regulation of water discharge release by the TGP might have caused an increase in the species diversity of the animal community on the beaches.A significant difference in diversity indices was obtained before and after the TGP operation.Similarity indices also indicate a gradual increase in species numbers.Hence,a long-term project should be established to monitor the population fluctuations of the Yangtze vole(Microtus fortis),the striped field mouse and the Norway rat to safeguard against population outbreaks(similar to the Yangtze vole outbreak in 2007),which could cause crop damage to adjacent farmland,in addition to documenting the succession process of the small mammal community inhabiting the beaches of Dongting Lake. | Meiwen ZHANG Yong WANG Bo LI Cong GUO Guoxian HUANG Guo SHEN Xunjun ZHOU | 2014 | Integrative Zoology2014，9，3： | 3 |
| 9 | MPTP Induces Systemic Parkinsonism in Middle-Aged Cynomolgus Monkeys:Clinical Evolution and Outcomes显示文摘In this study,we developed a systemic PD model in middle-aged cynomolgus monkeys using individualized low-dose MPTP,to explore effective indicators for the early prediction of clinical outcomes.MPTP was not stopped until the animals showed typical PD motor symptoms on days 10 to 13 after MPTP administration when the Kurlan score reached 10;this abrogated the differences in individual susceptibility to MPTP.The clinical symptoms persisted,peaking on days 3 to 12 after MPTP withdrawal(rapid progress stage),and then the Kurlan score plateaued.A Kurlan score at the end of the rapid progress stage >15 reflected stable or slowly-progressive PD,while a score <15 indicated spontaneous recovery.The entire clinical evolution and outcome of the systemic PD model was characterized in this study,thus providing options for therapeutic and translational research. | Feng Yue Sien Zeng Rongping Tang Guoxian Tao Piu Chan | 2017 | Neuroscience Bulletin2017，33，1： | 3 |
| 10 | Scope and Mechanism on Iridium-f-Amphamide Catalyzed Asymmetric Hydrogenation of Ketones显示文摘 | Zhiqin Liang Tilong Yang Guoxian Gu Li Dang Xumu Zhang | 2018 | Chinese Journal of Chemistry2018，36，9： | 3 |
| 11 | Characteristics of sedimentation and channel adjustment linked to the Three Gorges Reservoir显示文摘Construction of large dams is attractive because of their great benefits in flood control,hydropower generation,water resources utilization,navigation improvement,etc.However,dam construction may bring some negative impacts on sediment transport and channel dynamics adjustments.Due to the effects of recent water and soil conservation projects,sediment retention in the newly constructed large upstream reservoirs,and other factors,the sedimentation in the Three Gorges Reservoir(TGR)is quite different from the amount previously predicted in the demonstration stage.Consequently,based on the measured data,characteristics of sedimentation and the related channel deformation in the TGR were analyzed.The results imply that sediment transport tended to be reduced after the Three Gorges Project(TGP).Sedimentation slowed dramatically after 2013 and indicated obvious seasonal characteristics.Due to the rising water level in the TGR in the flood season,the yearly sediment export ratio(Eratio)was prone to decrease.The water level near the dam site should be reasonably regulated according to the flow discharge to improve the sediment delivery capacity and reduce sedimentation in the TGR,and to try to avoid situations where the flood retention time is close to 444 h.The depositional belt was discontinuous in the TGR and was mainly distributed in the broad reaches,and only slight erosion or deposition occurred in the gorge reaches.Sedimentation in the broad and gorge reaches accounted for 93.8% and 6.2% of the total sedimentation,respectively.The estuarine reach located in the fluctuating backwater area experienced alternate erosion-deposition,with a slight accumulative deposition in the curved reach.Sedimentation mainly occurred in the perennial backwater area.The insight gained in this study can be conducive to directly understanding of large reservoir sedimentation and mechanism of channel adjustment in the reservoir region in the main channel of large river. | Xiaoya Tang Sichen Tong Guoxian Huang Guangxiang Xu Xinghua Li Kun Lei Shiming Yao | 2021 | International Journal of Sediment Research2021，36，2： | 3 |
| 12 | RHOMBUS THINKING METHOD AND ITS APPLICATION IN SCHEME DESIGN显示文摘Rhombus thinking, a new creative thinking method,is the combination of divergent thinking process and convergent thinking process,in which qualitative analysis is carried out before quantitative analysis This method tries to solve the bottle neck problem in intelligent CAD based on the extension theory The rhombus thinking method to the scheme design of new products is applied In this process, firstly, the matter element expression for the know information are set up, and then a set of matter elements are opened up by matter elements extension method; Finally,the useful information are got by appraisal method of dependent degree It has been successfully applied to the scheme design for the cutter store of machining center Theoretical and experimental results demonstrated fhat the method is much more accurate,objective and efficient than the traditional | Zhao Yanwei Wang Wanliang Science Research Center,Zhejiang University of Technology Zhang Guoxian Shanghai University | 2001 | Chinese Journal of Mechanical Engineering2001，14，2： | 3 |
| 13 | Microstructure and Mechanical Property of 2024 Aluminium Alloy Prepared by Rapid Solidification and Mechanical Milling显示文摘Rapidly solidified 2024 aluminium alloy powders were mechanically milled, then consolidated to bulk form. The microstructural changes of the powders in mechanical milling (MM) and consolidation process were characterized by X-ray diffraction analyses and transmission electron microscopy observations. The results showed that mechanical milling reduced the grain size to nanometer, dissolved the Al2Cu intermetallic compound into the aluminium matrix and produced an aluminium supersaturated solid solution. During consolidation process. the grain size increased to submicrometer, and the Al2Cu and Al2(Cu, Mg, Si, Fe, Mn) compounds precipitated owing to heating. Increasing consolidation temperature and time results in obvious grain growth and coarsening of second phase particles. The tensile yield strength of the consolidated alloy with submicrometer size grains increases with decreasing grain size, and it follows the famous HallPetch | Guoxian LIANG Erde WANG Zhimin LI and Zhichao LI (School of Materials Science and Engineering, Harbin Institute of Technology Harbin, 150001, China) | 1995 | Journal of Materials Science & Technology1995，11，6： | 2 |
| 14 | Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson’s disease using nano-based microelectrode arrays显示文摘Parkinson’s disease(PD)is characterized by a progressive degeneration of nigrostriatal dopaminergic neurons.The precise mechanisms are still unknown.Since the neuronal communications are inherently electrical and chemical in nature,dual-mode detection of PD-related neuroelectrical and neurochemical information is essential for PD research.Subthalamic nucleus(STN)highfrequency stimulation(HFS)can improve most symptoms of PD patients and decrease the dosage of antiparkinsonian drugs.The mechanism of STN-HFS for PD still remains elusive.In this study,a silicon-based dual-mode microelectrode array(MEA)probe was designed and fabricated,and systematic dual-mode detection methods were established.The recording sites were modified using Pt nanoparticles and Nafion to improve the signal-to-noise(SNR)ratio.To evaluate its applicability to PD research,in vivo electrophysiological and electrochemical detection was performed in normal and hemiparkinsonian models,respectively.Through comparison of the dual-mode signals,we demonstrated the following in a PD monkey:(1)the maximum dopamine concentration in the striatum decreased by 90%;(2)the spike firing frequency increased significantly,especially in the region of the cortex;(3)the spectrogram analysis showed that much power existed in the 0–10 Hz frequency band;and(4)following repeated subthalamic nucleus high-frequency stimulation trials,the level of DA in the striatum increased by 16.5μM,which led to a better elucidation of the mechanism of HFS.The dual-mode MEA probe was demonstrated to be an effective tool for the study of neurological disorders. | Song Zhang Yilin Song Mixia Wang Guihua Xiao Fei Gao Ziyue Li Guoxian Tao Ping Zhuang Feng Yue Piu Chan Xinxia Cai | 2018 | Microsystems & Nanoengineering2018，4，1： | 2 |
| 15 | Hot Hydrostatic Extrusion of Mechanically Alloyed Al-4.9Fe-4.9Ni Powders显示文摘Al-4.9 Fe-4.9 Ni alloy powders have been synthesized by mechanical alloying. The rnechanically alloyed powders are consolidated by hot hydrostatic extrusion. The results show that extrusion tempereture. extrusion ratio and lubricant have great effects on the quality of extruded rods and their mechanical properties, The mixture of graphite and glass powders as lubricant can prevent the oxidization of cold compacted billet by cladding the billet with this lubricant before heating. This technique greatly simplifies the conventional densification process of | Guoxian LIANG Zhimin LI and Erde WANG (Dept. of Metals Science and Technology, Harbin Institute of Technology, Harbin, 150001, China)(To whom correspondence should be addressed) | 1994 | Journal of Materials Science & Technology1994，10，4： | 2 |
| 16 | Neuroprotective effects of salidroside against beta-amyloid-induced oxidative stress in SH-SY5Y human neuroblastoma cells显示文摘 | Li Zhang Huixin Yu Xincan Zhao Xiufeng Lin Chen Tan Guoxian Cao Zhengwu Wang | 2010 | Neurochemistry International2010，，5： | 2 |
| 17 | A new integrated design method based on fuzzy matter-element optimization显示文摘 | ZHAO Yanwei ZHANG Guoxian | 2002 | Journal of Materials Processing Technology2002，129，13： | 1 |
| 18 | Microwave-assisted synthesis of graphene-Ni composites with enhanced mi- crowave absorption properties in Ku-band 显示文摘 | Zetao Zhu Xin Sun Guoxian Li | 2015 | Journal of Magnetism and Magnetic Materials2015，377，： | 1 |
| 19 | High grade carcino ma of the proximal ureter with negative nephroureteroseopy detected by a positive fish test: a rare case report 显示文摘 | Guoxian Z Daniel S Miezcyslaw G | 2015 | Urology Case Reports2015，6，9： | 1 |
| 20 | Improving the perfor-nance of spring-supported thrust bearing by controlling its deformations显示文摘 | Wang Xiaojing Zhang Zhiming Zhang Guoxian | | 0，，12： | 1 |
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