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| 1 | Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study显示文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans. | Li-Li Ren Ye-Ming Wang Zhi-Qiang Wu Zi-Chun Xiang Li Guo Teng Xu Yong-Zhong Jiang Yan Xiong Yong-Jun Li Xing-Wang Li Hui Li Guo-Hui Fan Xiao-Ying Gu Yan Xiao Hong Gao Jiu-Yang Xu Fan Yang Xin-Ming Wang Chao Wu Lan Chen Yi-Wei Liu Bo Liu Jian Yang Xiao-Rui Wang Jie Dong Li Li Chao-Lin Huang Jian-Ping Zhao Yi Hu Zhen-Shun Cheng Un-Lin Liu Zhao-Hui Qian Chuan Qin Qi Jin Bin Cao Jian-Wei Wang | 2020 | Chinese Medical Journal2020，，9： | 99 |
| 2 | Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms显示文摘这篇文章在开发,基因疾病和癌症考察分子的结构,表达式模式,生理的函数,病理学的角色和 Twist1 的分子的机制。Twist1 是基本 helix-loop-helix 包含域的抄写因素。以便绑 Nde1 电子盒子元素并且激活或镇压形成 homo-dimers 或 hetero-dimers 它的目标基因。在开发期间, Twist1 为中层说明和区别是必要的。人的 Twist1 基因的异质接合的 loss-of-function 变化包括 Saethre-Chotzen 症候群引起几疾病。Twist1 空的老鼠胚胎与打开的头部的神经试管和有缺点的头间充组织死,体节和手足芽。Twist1 在胸被表示,肝,前列腺,胃并且癌症的另外的类型,和它的表示通常与侵略、变形的癌症显型被联系。在癌症房间, Twist1 是由包括 SRC-1 的多重因素的 upregulated, STAT3, MSX2, HIF-1α,连接 integrin 的 kinase 和 NF-κ B。Twist1 显著地提高上皮间充质的转变(EMT ) 和癌症房间移植和侵略,因此支持癌症转移。Twist1 由直接镇压部分地支持 EMT 由招募的 E-cadherin 表示改变的 nucleosome 和为基因压抑并且由 upregulating Bmi1 的 deacetylase 建筑群, AKT2, YB-1,等等。新兴的证据也建议 Twist1 在扩大和癌症干细胞的化学疗法的抵抗起一个作用。Twist1 由支持 Twist1 功能的调节的人的转移和鉴定的机制的进一步的理解可以为开发新策略禁止 EMT 和癌症转移保持诺言。 | Qian Qin Young Xu Tao He Chunlin Qin Jianming Xu | 2012 | Cell Research2012，22，1： | 66 |
| 3 | Programmed death-1 upregulation is correlated with dysfunction of tumor-infiltrating CD8^(+ ) T lymphocytes in human non-small cell lung cancer显示文摘T-cell tolerance is an important mechanism for tumor escape,but the molecular pathways involved in T-cell tolerance remain poorly understood.It remains unknown whether the inhibitory immunoreceptor programmed death-1(PD-1)plays a role in conditions of human non-small cell lung cancer(NSCLC).In this study,we detected PD-1 expression on CD81 T cells from healthy control peripheral blood mononuclear cells(PBMCs)and the PBMCs of NSCLC patients as well as NSCLC tissues.Results showed that tumor-infiltrating CD81 T cells had increased PD-1 expression and impaired immune function,including reducing cytokine production capability and impairing capacity to proliferate.Blockade of the PD-1/PD-L1 pathway by the PD-L1-specific antibody partially restored cytokine production and cell proliferation.These data provide direct evidence that the PD-1/PD-L1 pathway is involved in CD81 T-cell dysfunction in NSCLC patients.Moreover,blocking this pathway provides a potential therapy target in lung cancer. | Yan Zhang Shengdong Huang Dejun Gong Yanghua Qin Qian Shen | 2010 | Cellular & Molecular Immunology2010，7，5： | 48 |
| 4 | Probiotics in Helicobacter pylori eradication therapy: A systematic review and meta-analysis显示文摘AIM: To summarize the evidence from randomized controlled trials(RCTs) regarding the effect of probiotics by using a meta-analytic approach.METHODS: In July 2013, we searched PubM ed, EMBASE, Ovid, the Cochrane Library, and three Chinese databases(Chinese Biomedical Literature Database, Chinese Medical Current Content, and Chinese Scientific Journals database) to identify relevant RCTs.We included RCTs investigating the effect of a combination of probiotics and standard therapy(probiotics group) with standard therapy alone(control group).Risk ratios(RRs) were used to measure the effect of probiotics plus standard therapy on Helicobacter pylori(H.pylori) eradication rates, adverse events, and patient compliance using a random-effect model.RESULTS: We included data on 6997 participants from 45 RCTs, the overall eradication rates of the probiotic group and the control group were 82.31% and 72.08%, respectively.We noted that the use of probiotics plus standard therapy was associated with an increased eradication rate by per-protocol set analysis(RR = 1.11; 95%CI: 1.08-1.15; P < 0.001) or intention-totreat analysis(RR = 1.13; 95%CI: 1.10-1.16; P < 0.001).Furthermore, the incidence of adverse events was 21.44% in the probiotics group and 36.27% in the control group, and it was found that the probiotics plus standard therapy significantly reduced the risk of adverse events(RR = 0.59; 95%CI: 0.48-0.71; P < 0.001), which demonstrated a favorable effect of probiotics in reducing adverse events associated with H.pylori eradication therapy.The specific reduction in adverse events ranged from 30% to 59%, and this reduction was statistically significant.Finally, probiotics plus standard therapy had little or no effect on patient compliance(RR = 0.98; 95%CI: 0.68-1.39; P = 0.889).CONCLUSION: The use of probiotics plus standard therapy was associated with an increase in the H.pylori eradication rate, and a reduction in adverse events resulting from treatment in the general population.However, this therapy did not improve patient compliance. | Min-Min Zhang Wei Qian Ying-Yi Qin Jia He Yu-Hao Zhou | 2015 | World Journal of Gastroenterology2015，21，14： | 41 |
| 5 | Prevalence, risk factors, clinical course, and outcome of acute kidney injury in Chinese intensive care units: a prospective cohort study显示文摘 | WEN Ying JIANG Li XU Yuan QIAN Chuan-yun LI Shu-sheng QIN Tie-he CHEN Er-zhen LIN Jian-dong AI Yu- hang WU Da-wei WANG Yu-shan SUN Ren-hua HU Zhen-jie CAO Xiang-yuan ZHOU Fa-chun HE Zhen-yang ZHOU Li-hua AN You-zhong KANG Yan MA Xiao-chun YU Xiang-you ZHAO Ming-yan XI Xiu-ming DU Bin | 2013 | Chinese Medical Journal2013，，23： | 40 |
| 6 | Linking atmospheric pollution to cryospheric change in the Third Pole region: current progress and future prospects显示文摘The Tibetan Plateau and its surroundings are known as the Third Pole(TP). This region is noted for its high rates of glacier melt and the associated hydrological shifts that affect water supplies in Asia. Atmospheric pollutants contribute to climatic and cryospheric changes through their effects on solar radiation and the albedos of snow and ice surfaces; moreover, the behavior and fates within the cryosphere and environmental impacts of environmental pollutants are topics of increasing concern. In this review, we introduce a coordinated monitoring and research framework and network to link atmospheric pollution and cryospheric changes(APCC) within the TP region. We then provide an up-to-date summary of progress and achievements related to the APCC research framework, including aspects of atmospheric pollution’s composition and concentration, spatial and temporal variations, trans-boundary transport pathways and mechanisms, and effects on the warming of atmosphere and changing in Indian monsoon, as well as melting of glacier and snow cover. We highlight that exogenous air pollutants can enter into the TP’s environments and cause great impacts on regional climatic and environmental changes. At last, we propose future research priorities and map out an extended program at the global scale. The ongoing monitoring activities and research facilitate comprehensive studies of atmosphere–cryosphere interactions, represent one of China’s key research expeditions to the TP and the polar regions and contribute to the global perspective of earth system science. | Shichang Kang Qianggong Zhang Yun Qian Zhenming Ji Chaoliu Li Zhiyuan Cong Yulan Zhang Junming Guo Wentao Du Jie Huang Qinglong You Arnico K.Panday Maheswar Rupakheti Deliang Chen Orjan Gustafsson Mark H.Thiemens Dahe Qin | 2019 | National Science Review2019，6，4： | 35 |
| 7 | Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid显示文摘瞄准:现在的学习被设计决定位于三氧化二砷的联合使用导致的 apoptosis 的改进下面的可能的小径(作为(2 ) O (3 )) 并且抗坏血酸(AA ) 。方法:细胞内部的反应的氧种类(ROS ) 的水平与上载的一根氧化敏感的荧光灯的探针(6-carboxy-2',7' dichlorodihydrofluorescein 乙酰乙酸盐) 借助于流动血细胞计数分析被检测。谷胱甘肽(GSH ) 的活动,谷胱甘肽过氧化物酶(GPx ) ,和超级氧化物歧化酶(草皮) 被生物化学的方法检测。mitochondrial 膜电位被流动血细胞计数分析与玫瑰精 123 测量染色。caspase-3 的 Bcl-2, Bax,和 p17 子单元用西方的污点方法被分析。apoptosis 率被流动血细胞计数与 annexin-V/propidium 碘化物染色决定。结果:与相比当( 2 ) O ( 3 )( 2.0 micromol/L )独自对待,( 2 )在有 AA ( 100 micromol/L )的联合的 O ( 3 )( 2.0 micromol/L )减少了从 101.30+/-5.76 的细胞内部的 GSH 内容到 81.91+/-3.12 mg/g 蛋白质,并且增加了 ROS 从 127.61+/-5.12 铺平到 152.60+/-5.88 ,它被代表由 2 , 7-dichlorofluorescein 紧张。线粒体膜电位的损失从 1269.97+/-36.11 被增加到 1540.52+/-52.63,它被荧光紧张介绍。caspase-3 表示的 p17 子单元被增加近似 2 褶层。然而,草皮和 GPx 弄空和到 Bax 的 Bcl-2 的比率等于独自对待的 As2O3 (P>0.05 ) 的。什么时候 ROS 清道工人, N-acetyl-L-cysteine,被加到当(2 ) O (3 ) 和 AA 联合了处理组, apoptosis 率从 15.60 %+/-1.14% 减少了到 9.48%+/-0.67% ,和 ROS 水平从 152.60+/-5.88 减少了到 102.77+/-10.25。结论:当(2 ) O (3 ) 由增加 ROS 水平通过氧化小径导致了 apoptosis, AA 加强了。这可以是弄空的结果细胞内部的 GSH。它可以影响下游的串联追随者 ROS,包括线粒体去极和 caspase-3 激活。然而,草皮和 GPx 弄空和到 Bax 的 Bcl-2 的比率影响了由因为(2 ) O (3 ) 是,别发现被 AA 加强。 | Jing-jing LI Qiang TANG Yuan LI Ben-rong HU Zhang-yin MING Qin FU Jia-qing QIAN Ji-zhou XIANG | 2006 | Acta Pharmacologica Sinica2006，27，8： | 25 |
| 8 | Root microbiota shift in rice correlates with resident time in the field and developmental stage显示文摘Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota composition has been widely studied in several model plants and crops; however, little is known about how root microbiota vary throughout the plant's life cycle under field conditions. We performed longitudinal dense sampling in field trials to track the time-series shift of the root microbiota from two representative rice cultivars in two separate locations in China. We found that the rice root microbiota varied dramatically during the vegetative stages and stabilized from the beginning of the reproductive stage, after which the root microbiota underwent relatively minor changes until rice ripening. Notably, both rice genotype and geographical location influenced the patterns of root microbiota shift that occurred during plant growth. The relative abundance of Deltaproteobacteria in roots significantly increased overtime throughout the entire life cycle of rice, while that of Betaproteobacteria, Firmicutes, and Gammaproteobacteria decreased. By a machine learning approach, we identified biomarker taxa and established a model to correlate root microbiota with rice resident time in the field(e.g., Nitrospira accumulated from 5 weeks/tillering in field-grown rice). Our work provides insights into the process of rice root microbiota establishment. | Jingying Zhang Na Zhang Yong-Xin Liu Xiaoning Zhang Bin Hu Yuan Qin Haoran Xu Hui Wang Xiaoxuan Guo Jingmei Qian Wei Wang Pengfan Zhang Tao Jin Chengcai Chu Yang Bai | 2018 | Science China(Life Sciences)2018，61，6： | 24 |
| 9 | Roles of nitric oxide in protective effect of berberine in ethanol-induced gastric ulcer mice显示文摘瞄准:在老鼠在导致乙醇的胃溃疡上调查小檗硷的保护的效果。方法:胃溃疡被乙醇的口头的摄取导致。氮的氧化物(没有) 内容被测量,并且内皮的氮的氧化物 synthase (eNOS ) 的 mRNA 表示和可诱导的氮的氧化物 synthase (i NOS ) 被反向的抄写聚合酶链反应(RT-PCR ) 分析。结果:在在乙醇的口服以后的 1 h, 2 h, 3 h 和 6 h 的溃疡索引(UI ) 分别地是 23.8+/-1.4, 23.3+/-2.2, 22.3+/-1.2 和 20.8+/-1.1。在对待 berberine 的组(5 mg/kg 和 50 mg/kg ) 的 UI 是不到控制组。内容不在控制组是在胃的果汁和 5.8+/-1.1 micromol/g 的 73.3+/-7.3 microL/L , 94.0+/-9.2 microL/L , 109.6+/-6.4 microL/L 和 138.2+/-10.2 microL/L 在在 1 h , 2 h , 3 h 和 6 h 的胃的织物的蛋白质, 8.3+/-1.1 micromol/g 蛋白质, 9.8+/-1.1 micromol/g 蛋白质和 11.9+/-1.2 micromol/g 蛋白质,分别地,在乙醇的口服以后。内容不在对待 berberine 的组(5 mg/kg 和 50 mg/kg ) 比在在乙醇的口服以后的 1 h | Long-rui PAN Qiang TANG Qin FU Ben-rong HU Ji-zhou XIANG Jia-qing QIAN | 2005 | Acta Pharmacologica Sinica2005，26，11： | 23 |
| 10 | Ten cities cross-sectional questionnaire survey of children asthma and other allergies in China显示文摘Asthma,rhinitis and eczema(allergic or non-allergic)have increased throughout the world during the last decades,especially among children.Changes in the indoor environment are suspected to be important causes.China has experienced a dramatic change in indoor environmental exposures during the past two decades.However,such changes and their associations with children’s asthma and other health aspects have not been thoroughly studied.China,Children,Homes,Health(CCHH),Phase I,was a cross-sectional questionnaire survey of 48219 children 1–8 years old in 10 Chinese cities during 2010–2012.The questionnaire includes the International Study of Asthma and Allergies in Childhood(ISAAC)core health questions and additional questions regarding housing,life habits and outdoor environment.In health analyses,children aged 3–6 years old were included.The prevalences of doctor diagnosed asthma varied from 1.7%to 9.8%(mean 6.8%),a large increase from 0.91%in 1999 and 1.50%in2000.The prevalence of wheeze,rhinitis and atopic eczema(last 12 months)varied from 13.9%to 23.7%,24.0%to 50.8%and4.8%to 15.8%,respectively.Taiyuan had the lowest prevalences of all illnesses and Shanghai the highest,except for wheezewhere the highest value was for Urumqi.We found(1)no obvious association between disease prevalences and ambient PM10concentrations and(2)higher prevalences of disease in humid climates with hot summers and cold winters,but with no centrally heated buildings.Associations between the diseases and economic status as indexed by Gross Domestic Product(GDP)requires further study. | ZHANG YinPing LI BaiZhan HUANG Chen YANG Xu QIAN Hua DENG QiHong ZHAO ZhuoHui LI AnGui ZHAO JiaNing ZHANG Xin QU Fang HU Yu YANG Qin WANG Juan ZHANG Ming WANG Fang ZHENG XiaoHong LU Chan LIU ZhiJian SUN YueXia MO JinHan ZHAO YiLi LIU Wei WANG TingTing NORBCK Dan BORNEHAG Carl-Gustaf SUNDELL Jan | 2013 | Chinese Science Bulletin2013，58，34： | 22 |
| 11 | Clinical significance of serum intercellular adhesion molecule-1 detection in patients with hepatocellular carcinoma显示文摘INTRODUCTION Since the late 1980s,studies on the expression ofintercellular adhesion molecule-1(ICAM-1)inpatients with malignancies have demonstrated thatICAM-1 may strongly express in two forms in suchdiseases:membranous one on the surface of tumorcells(membrane-bound ICAM-1)and soluble one incirculation(soluble ICAM-1,sICAM-1). | Ming Hui Mei Jing Xu Qin Fen Shi Jing Hong Yang Qian CHen Li Ling Qin Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery,Guilin Medical College,Guilin 541001,Guangxi Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 20 |
| 12 | Epidemiological characteristics of thyroid nodules and risk factors for malignant nodules: a retrospective study from 6 304 surgical cases显示文摘 | Huan Qin Wang Kun Lou Fuchen Zhang Li Huang Qingxian Han Yunfeng Sun Hancheng Zhu Lei Lin Peng Song Jun Liu Fuqiang Wang Qian Hou Weikai | 2014 | Chinese Medical Journal2014，，12： | 20 |
| 13 | A practical guide to amplicon and metagenomic analysis of microbiome data显示文摘Advances in high-throughput sequencing(HTS)have fostered rapid developments in the field of microbiome research,and massive microbiome datasets are now being generated.However,the diversity of software tools and the complexity of analysis pipelines make it difficult to access this field.Here,we systematically summarize the advantages and limitations of microbiome methods.Then,we recommend specific pipelines for amplicon and metagenomic analyses,and describe commonly-used software and databases,to help researchers select the appropriate tools.Furthermore,we introduce statistical and visualization methods suitable for microbiome analysis,including alpha-and betadiversity,taxonomic composition,difference comparisons,correlation,networks,machine learning,evolution,source tracing,and common visualization styles to help researchers make informed choices.Finally,a stepby-step reproducible analysis guide is introduced.We hope this review will allow researchers to carry out data analysis more effectively and to quickly select the appropriate tools in order to efficiently mine the biological significance behind the data. | Yong-Xin Liu Yuan Qin Tong Chen Meiping Lu Xubo Qian Xiaoxuan Guo Yang Bai | 2021 | Protein & Cell2021，12，5： | 20 |
| 14 | Precise nanomedicine for intelligent therapy of cancer显示文摘Precise nanomedicine has been extensively explored for efficient cancer imaging and targeted cancer therapy, as evidenced by a few breakthroughs in their preclinical and clinical explorations. Here, we demonstrate the recent advances of intelligent cancer nanomedicine, and discuss the comprehensive understanding of their structure-function relationship for smart and efficient cancer nanomedicine including various imaging and therapeutic applications, as well as nanotoxicity. In particular, a few emerging strategies that have advanced cancer nanomedicine are also highlighted as the emerging focus such as tumor imprisonment, supramolecular chemotherapy, and DNA nanorobot. The challenge and outlook of some scientific and engineering issues are also discussed in future development. We wish to highlight these new progress of precise nanomedicine with the ultimate goal to inspire more successful explorations of intelligent nanoparticles for future clinical translations. | Huabing Chen Zhanjun Gu Hongwei An Chunying Chen Jie Chen Ran Cui Siqin Chen Weihai Chen Xuesi Chen Xiaoyuan Chen Zhuo Chen Baoquan Ding Qian Dong Qin Fan Ting Fu Dayong Hou Qiao Jiang Hengte Ke Xiqun Jiang Gang Liu Suping Li Tianyu Li Zhuang Liu Guangjun Nie Muhammad Ovais Daiwen Pang Nasha Qiu Youqing Shen Huayu Tian Chao Wang Hao Wang Ziqi Wang Huaping Xu Jiang-Fei Xu Xiangliang Yang Shuang Zhu Xianchuang Zheng Xianzheng Zhang Yanbing Zhao Weihong Tan Xi Zhang Yuliang Zhao | 2018 | Science China Chemistry2018，61，12： | 19 |
| 15 | Exogenous NADPH ameliorates myocardial ischemia–reperfusion injury in rats through activating AMPK/mTOR pathway显示文摘Our previous study shows that nicotinamide adenine dinucleotide phosphate(NADPH)plays an important role in protecting against cerebral ischemia injury.In this study we investigated whether NADPH exerted cardioprotection against myocardial ischemia/reperfusion(I/R)injury.To induce myocardial I/R injury,rats were subjected to ligation of the left anterior descending branch of coronary artery for 30 min followed by reperfusion for 2 h.At the onset of reperfusion,NADPH(4,8,16 mg·kg?1·d?1,iv)was administered to the rats.We found that NADPH concentrations in plasma and heart were signi?cantly increased at 4 h after intravenous administration.Exogenous NADPH(8?16 mg/kg)signi?cantly decreased myocardial infarct size and reduced serum levels of lactate dehydrogenase(LDH)and cardiac troponin I(cTn-I).Exogenous NADPH signi?cantly decreased the apoptotic rate of cardiomyocytes,and reduced the cleavage of PARP and caspase-3.In addition,exogenous NADPH reduced mitochondrial vacuolation and increased mitochondrial membrane protein COXIV and TOM20,decreased BNIP3L and increased Bcl-2 to protect mitochondrial function.We conducted in vitro experiments in neonatal rat cardiomyocytes(NRCM)subjected to oxygen–glucose deprivation/restoration(OGD/R).Pretreatment with NADPH(60,500 nM)signi?cantly rescued the cell viability and inhibited OGD/R-induced apoptosis.Pretreatment with NADPH signi?cantly increased the phosphorylation of AMPK and downregulated the phosphorylation of mTOR in OGD/R-treated NRCM.Compound C,an AMPK inhibitor,abolished NADPH-induced AMPK phosphorylation and cardioprotection in OGD/R-treated NRCM.In conclusion,exogenous NADPH exerts cardioprotection against myocardial I/R injury through the activation of AMPK/mTOR pathway and inhibiting mitochondrial damage and cardiomyocyte apoptosis.NADPH may be a potential candidate for the prevention and treatment of myocardial ischemic diseases. | Jiang Zhu Yi-fei Wang Xiao-ming Chai Ke Qian Ling-wei Zhang Peng Peng Pei-min Chen Jian-fang Cao Zheng-hong Qin Rui Sheng Hong Xie | 2020 | Acta Pharmacologica Sinica2020，41，4： | 19 |
| 16 | Electroacupuncture for the prevention of postoperative gastrointestinal dysfunction in patients undergoing vascular surgery under general anesthesia: study protocol for a prospective practical randomized controlled trial显示文摘BACKGROUND: Postoperative gastrointestinal dysfunction(PGD) is one of the most common complications following major surgeries under general anesthesia(GA). Despite ongoing research and new drug treatments, abdominal distension within 24 h postoperatively occurs in 8%–28% of all surgeries. We aim to analyze the effectiveness of preventing PGD by preoperatively stimulating Neiguan(PC6), Zusanli(ST36) and Shangjuxu(ST37) bilaterally twice a day compared with sham-acupuncture treatment and standard treatment.METHODS AND DESIGN: This is a single-center, prospective practical randomized controlled trial. All groups will be given standard treatments. Patients undergoing vascular surgery under GA will be included from the Vascular Surgery Unit in West China Hospital of Sichuan University, China, and divided into three groups. The experimental group will receive routine treatments and acupuncture at PC6, ST36 and ST37 bilaterally with electrical stimulation twice a day for 20 min preoperatively. The sham-acupuncture group will receive pseudo-electroacupuncture at sham acupoints of PC6, ST36 and ST37, which are 1 cun away from the real acupoints. The routine-treatment group will not receive electroacupuncture. The outcomes include the incidence of abdominal distention, abdominal circumference, the degree of abdominal distension, the fi rst time of fl atus and defecation, and hospitalization duration. DISCUSSION: The results from this study will demonstrate whether preoperative electroacupuncture is an effective method for the prevention of PGD in patients undergoing vascular surgery under GA. This study may also provide a standardized acupuncture treatment for reduction of PGD. TRIAL REGISTRATION: This study is registered with the Chinese Clinical Trial Registry: Chi CTR-TRC-13003649. | Ying Li Hui Zheng Qian-hua Zheng Ling Zhao Er-qi Qin Yu Wang Qian Zeng Hua-bin Zheng Yu Zhao Wei Sun Xiao-xia Zhang Zhi-shun Liu Bao-yan Liu | 2014 | Journal of Integrative Medicine2014，12，6： | 19 |
| 17 | Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis显示文摘 | Yongting Luo Hongxia Duan Yining Qian Liqun Feng Zhenzhen Wu Fei Wang Jing Feng Dongling Yang Zhihai Qin Xiyun Yan | 2017 | Cell Research2017，27，3： | 18 |
| 18 | Salidroside inhibits H2O2-induced apoptosis in PC12 cells by preventing cytochrome c release and inactivating of caspase cascade显示文摘我们使用了老鼠 pheochromocytoma (PC 12 ) 在氢过氧化物上学习 salidroside 的效果的房间线(H 2 O 2)-induced apoptosis。在 PC12 房间, H 2 O 2-induced apoptosis 被 Bcl-2, Bax 的起来规定,到 cytosol 的 mitochondrial 细胞色素 c 的版本,和 caspase-3, -8 和 -9 的激活的下面规定伴随。然而, salidroside 压制了 Bcl-2, Bax 的起来规定和 mitochondrial 细胞色素 c 的版本的下面规定到 cytosol。而且, salidroside 对 H 2 O 2-induced apoptosis。总起来说,这些结果建议有 salidroside 的 PC12 房间的处理能堵住 H 2 由调整 Bcl-2 家庭成员并且由压制细胞色素 c 版本和 caspase 串联激活的 O 2-induced apoptosis。 | Lei Cai Hua Wang Qin Li Yunfei Qian Wenbing Yao | 2008 | Acta Biochimica et Biophysica Sinica2008，40，9： | 18 |
| 19 | Clinical and laboratory survey of 65 Chinese patients with Leigh syndrome显示文摘Background Leigh syndrome is an inherited neurodegenerative disease that emerges in infancy and childhood and presents with a clinically heterogeneous variety of neuromuscular and non-neuromuscular disorders. It can result from the inheritance of mutations in either nuclear or mitochondrial DNA. In the current study, we performed a retrospective study in 65 patients in order to investigate the clinical and genetic characteristics of Leigh syndrome in Chinese patients. Methods Sixty-five unrelated cases (35 men and 30 women) who were hospitalized in the past 12 years were reviewed. Diagnosis was based on both the clinical presentation and the characteristic neuropathologic findings of bilateral symmetric necrotizing lesions in the basal ganglia and brain stem as detected using cranial computed tomography (CT) scan or magnetic resonance imaging (MRI). The differential diagnosis of organic acidurias and fatty acid ?-oxidation defects were performed. Specific point mutations and deletions in mitochondrial DNA (T8993G, T8993C, T9176C, A8344G, A3243G) were screened by PCR-restriction analysis and Southern blot. The SURF1 gene was sequenced. Skeletal muscle biopsies were performed in 17 (26.2%) of the patients. The diagnosis was confirmed by autopsy in 6 (9.2%) patients. Results The patients had various forms of metabolic encephalomyopathy. Fifty-nine (90.8%) of the patients had the typical neuroradiological features of Leigh syndrome, including symmetrical necrotizing lesions scattered within the basal ganglia, thalamus and brain stem. Twenty (30.8%) patients were confirmed by genetic, biochemical analysis and autopsy. Specific point mutations in mitochondrial DNA were found in 5 cases (7.7%). Of these, the A8344G mutation was detected in 2 patients. The T8993G, T8993C, and A3243G point mutations were identified in 3 other patients, respectively. SURF1 mutations associated with cytochrome c oxidase deficiency were identified in 8 (12.3%) families by DNA sequencing. A G604C mutation was identified in 6 (9.2%) patients. The genotypes of 52 patients remained unknown. Conclusions Leigh syndrome presents as a diverse array of clinical features and can result from specific mutations in nuclear or mitochondrial DNA. In this study, SURF1 mutations associated with cytochrome c oxidase deficiency were identified in 8 (12.3%) out of 65 patients with Leigh syndrome. It indicates that SURF1 mutations might be a common cause of Leigh syndrome in China. The etiology of Leigh syndrome in Chinese patients represents a persistent challenge to clinicians. | YANG Yan-ling SUN Fang ZHANG Yao QIAN Ning YUAN Yun WANG Zhao-xia QI Yu XIAO Jiang-xi WANG Xiao-ying QI Zhao-yue ZHANG Yue-hua JIANG Yu-wu BAO Xin-hua QIN Jiong WU Xi-ru | 2006 | Chinese Medical Journal2006，，5： | 17 |
| 20 | Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor显示文摘According to the World Health Organization(WHO)newly updated situation report on March 18th,2020,the coronavirus disease 2019(COVID-19)pandemic has confirmed 191,127 cases and claimed 7807 deaths worldwide.1 The etiological agent of COVID-19 has been identified as a novel coronavirus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to Sarbecovirus subgenus(genus Betacoronavirus,family Coronaviridae)and showing 79.6 and 96.2%sequence identity in nucleotide to SARS-CoV and a bat coronavirus(BatCoV RaTG13),respectively.2–4 Like SARS-CoV infection,a substantial fraction of COVID-19 patients exhibits severe respiratory symptoms and has to be hospitalized in intensive care unit.5–8 Although the mortality rate of COVID-19 is significantly lower than that of SARS-CoV infection,SARS-CoV-2 shows much higher human-to-human transmission rate,rapidly leading to a global pandemic declared by WHO on March 11th,2020. | Xiangyu Chen Ren Li Zhiwei Pan Chunfang Qian Yang Yang Renrong You Jing Zhao Pinghuang Liu Leiqiong Gao Zhirong Li Qizhao Huang Lifan Xu Jianfang Tang Qin Tian Wei Yao Li Hu Xiaofeng Yan Xinyuan Zhou Yuzhang Wu Kai Deng Zheng Zhang Zhaohui Qian Yaokai Chen Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，6： | 17 |
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