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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | Resveratrol provides neuroprotection by regulating the JAK2/STAT3/PI3K/AKT/mTOR pathway after stroke in rats显示文摘Ischemic stroke is a common disease with high mortality and morbidity worldwide.One of the important pathophysiological effects of ischemic stroke is apoptosis.A neuroprotective effect is defined as the inhibition of neuronal apoptosis to rescue or delay the infarction in the surviving ischemic penumbra.Resveratrol is a natural polyphenol that reportedly prevents cerebral ischemia injury by regulating the expression of PI3K/AKT/mTOR.Therefore,this study aimed to elucidate the neuroprotective effect of resveratrol on cerebral ischemia/reperfusion injury and to investigate the signaling pathways and mechanisms through which resveratrol regulates apoptosis in the ischemic penumbra.Rats were subjected to middle cerebral artery occlusion for 2 h followed by 24 h reperfusion.Cerebral infarct volume was measured using 2%TTC staining.TUNEL staining was conducted to evaluate neuronal apoptosis.Western blotting and immunohistochemistry were used to detect the proteins involved in the JAK2/STAT3/PI3K/AKT/mTOR pathway.The results suggested that resveratrol significantly improved neurological function,reduced cerebral infarct volume,decreased neuronal damage,and markedly attenuated neuronal apoptosis;these effects were attenuated by the inhibition of PI3K/AKT with LY294002 and JAK2/STAT3 with AG490.We also found that resveratrol significantly upregulated the expression of p-JAK2,p-STAT3,p-AKT,p-mTOR,and BCL-2 and downregulated expression of cleaved caspase-3 and BAX,which was partially reversed by LY294002 and AG490.These results suggested that resveratrol provides a neuroprotective effect against cerebral ischemia/reperfusion injury,which is partially mediated by the activation of JAK2/STAT3 and PI3K/AKT/mTOR.Resveratrol may indirectly upregulate the PI3K/AKT/mTOR pathway by activating JAK2/STAT3. | Yongying Hou Ke Wang Weijun Wan Yue Cheng Xia Pu Xiufeng Ye | 2018 | Genes & Diseases2018，5，3： | 60 |
| 3 | Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells显示文摘瞄准:在 mesangial 房间在 mesangial 房间的肥大和细胞外的矩阵(ECM ) 的累积上观察白果树 biloba 摘录(EGb ) 的效果。方法:有教养的 mesangial 房间被分派进 7 个组:正常的组,溶剂控制组,高葡萄糖组, EGb 的低剂量组织, EGb 组,的中等剂量高 EGb 组开,并且 captopril 组织。房间 antioxidases, S 阶段百分比和 G (0 ) 的活动 /G (1 ) 阶段百分比,骨胶原 IV 和 laminin, Smad2/3 和 Smad7, TGF 贝它(1 ) mRNA 被不同方法测量。结果:为对待 EGb 的组,当与高度相比葡萄糖组织时, S 阶段的房间百分比被提起, G (0 )/G (1 ) 的百分比被降低。氧化应力的紧张被削弱。Smad2/3 的表示极大地被减少, Smad7 被增加。骨胶原 IV, laminin 和 TGF- 贝它(1 ) mRNA 也被减少。结论:EGb 能在老鼠 mesangial 房间压制房间肥大和 ECM 的累积,它意味着它能在糖尿病的肾病在肾小球硬化症的延期起一个重要作用。 | Jian-yun WANG Xiao-xing YIN Yun-ming WU Dao-quan TANG Yuan-yuan GAO Mei-rong WAN Xiao-yu HOU Bei ZHANG | 2006 | Acta Pharmacologica Sinica2006，27，9： | 18 |
| 4 | Characteristics of coastline changes in China's Mainland since the early 1940s显示文摘Based on multi-temporal topographic maps, remote sensing images and field surveys covering the entire coastal zone of China's Mainland, the coastlines of six periods since the early 1940 s were extracted. Coastline changes over the last 70 years were then analyzed in terms of coastline structure, coastline fractals, coastline change rates, land-sea patterns, and bay areas. The results showed that mainland coastline structure changed dramatically, and due to the significant coastline artificialization mainly driven by sea reclamation and coastal engineering, the remaining natural coastline merely accounts for less than one third at present. Coastline fractal dimension represented an overall spatial pattern of 'north < entirety < south'; however, the discrepancy between the north and south coast was apparently narrowed due to dramatic coastline artificialization of northern China which in turn altered the whole pattern. Patterns and processes of land-sea interchange along the mainland coast were complex and varied spatially and temporally, with over 68% advancing toward sea and 22% retreating toward land. The net growth of land area was nearly 14.2 ×103 km2 with an average growth rate of 202.82 km2 a??; and coast retreat was characterized by area decrease of 93 bays with a magnitude of 10.1 ×103 km2 and an average shrinking rate up to 18.19% or an average shrinking speed up to 144.20 km2 a??, among which the total area of Bohai shrunk by 7.06%, with an average annual loss amounting to 82 km2. The dramatic coastline changes along China's Mainland have brought about kinds of challenges to the coastal environment, therefore the integrated management, effective environment protection and sustainable utilization of coastlines is urgent. | HOU XiYong WU Ting HOU Wan CHEN Qing WANG YuanDong YU LiangJu | 2016 | Science China Earth Sciences2016，59，9： | 18 |
| 5 | Astragaloside II triggers T cell activation through regulation of CD45 protein tyrosine phosphatase activity显示文摘 | Chun-ping WAN Li-xin GAO Li-fei HOU Xiao-qian YANG Pei-lan HE Yi-fu YANG Wei TANG Jian-min YUE Jia LI Jian-ping ZUO | 2013 | Acta Pharmacologica Sinica2013，34，4： | 17 |
| 6 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 7 | Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application显示文摘The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application.Early angiogenesis is important for scaffold survival.It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties.In this study,a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold.For osseointegration and osteogenesis analysis,in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation,adhesion,extracellular matrix(ECM)mineralization and ALP activity compared with bare Ti6Al4V cocultivation.Additionally,MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesisrelated genes expression.In vivo studies including fluorochrome labeling,micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation.For angiogenesis studies,magnesium-coated Ti6Al4V improve HUVECs proliferation,adhesion,tube formation,wound-healing and Transwell abilities.HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes(HIF-1αand VEGF)expression.Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation.This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications. | Peng Gao Bo Fan Xiaoming Yu Wenwen Liu Jie Wu Lei Shi Di Yang Lili Tan Peng Wan Yulin Hao Shujun Li Wentao Hou Ke Yang Xiaokang Li Zheng Guo | 2020 | Bioactive Materials2020，5，3： | 15 |
| 8 | Understanding hydraulic fracture propagation behavior in tight sandstone–coal interbedded formations: an experimentalinvestigation显示文摘Whether hydraulic fractures could connect multiple gas zones in the vertical plane is the key to fracturing treatment to jointly exploit coalbed methane and tight sandstone gas through integrative hydraulic fracturing in tight sandstone–coal interbedded formations. Laboratory true triaxial hydraulic fracturing experiments were conducted on layered specimens with di erent combination types of natural sandstone and coal to simulate the propagation behavior of hydraulic fractures. The effects of the fracture initiation position, fracturing fluid viscosity and injection rate were discussed. The results showed that di erent fracture morphologies could be found. When initiating from coal seams, three patterns of fracture initiation and propagation were obtained:(1) The main hydraulic fracture initiated and propagated along the natural fractures and then diverged due to the effects of in situ stress and formed secondary fractures.(2) The hydraulic fracture initiated and propagated in the direction of the maximum horizontal stress.(3) Multiple fractures initiated and propagated at the same time. With the same fracturing fluid viscosity and injection rate, the hydraulic fractures initiating in sandstones had greater chances than those in coal seams to penetrate interfaces and enter neighboring layers. Excessively small or large fracturing fluid viscosity and injection rate would do harm to the vertical extension height of the induced fracture and improvement of the stimulated reservoir volume. Compared with operation parameters(fracturing fluid viscosity and injection rate), the natural weak planes in coals were considered to be the key factor that a ected the fracture propagation path. The experimental results would make some contributions to the development of tight sandstone–coal interbedded reservoirs. | Peng Tan Yan Jin Liang Yuan Zhen-Yu Xiong Bing Hou Mian Chen Li-Ming Wan | 2019 | Petroleum Science2019，16，1： | 14 |
| 9 | Interpretation Criteria for Standardized Western Blot for the Predominant Species of Borrelia Burgdorferi Sensu Lato in China显示文摘Objective Western blotting (WB;immunoblotting) is a widely used tool for the serodiagnosis of Lyme borreliosis (LB),but so far,no generally accepted criteria for its performance and interpretation have been established in China.The present study was designed to determine the criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in China,in which WB was produced with strain PD91 as the representative strain attributed to predominant genospecies Borrelia garinii of Borrelia burgdorferi sensu lato.Methods Approximately 13 bands between 14 and 100 kD were differentiated for strain PD91 by using Gel-Pro analysis software.In a study with 631 serum samples (taken from 127 patients with Lyme borreliosis and 504 controls),all observed bands were documented.To establish criteria for a positive WB result for strain PD91,receiver operating characteristic (ROC) curves were used.Results The following interpretation criteria were recommended:for IgG,at least one band of P83/100,P58,P39,P30,OspC,P17,P66,and OspA;for IgM,at least one band of P83/100,P58,OspA,P30,OspC,P17 or P41.In addition,syphilis,leptospirosis and other related diseases should be excluded when the positive band is P41 in IgM.For IgG criteria,the sensitivity is 73.2%,the specificity is 99.4% and Youden index is 0.726;for IgM criteria,the sensitivity is 50.6%,the specificity is 93.1% and Youden index is 0.437.Conclusion Standardization of WB assays is necessary for comparison of results from different laboratories.Moreover,the criteria of other genospecies of Borrelia burgdorferi sensu lato should be determined in the future to complete the criteria of WB for the diagnosis of the Lyme disease in China. | YI JIANG XUE-XIA HOU ZHEN GENG QIN HAO KANG-LIN WAN | 2010 | Biomedical and Environmental Sciences2010，23，5： | 13 |
| 10 | Seroepidemiological Investigation of Lyme Disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China显示文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick‐borne diseases caused by Borrelia burgdorferi and Anaplasma phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay (IFA). Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces (the exception being the Miyun area in Beijing). The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces. | HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin | 2013 | Biomedical and Environmental Sciences2013，26，3： | 13 |
| 11 | Fluorination vs. chlorination: a case study on high performance organic photovoltaic materials显示文摘Halogenation is a very efficient chemical modification method to tune the molecular energy levels, absorption spectra and molecular packing of organic semiconductors. Recently, in the field of organic solar cells(OSCs), both fluorine-and chlorinesubstituted photovoltaic materials, including donors and acceptors, demonstrated their great potentials in achieving high power conversion efficiencies(PCEs), raising a question that how to make a decision between fluorination and chlorination when designing materials. Herein, we systemically studied the impact of fluorination and chlorination on the properties of resulting donors(PBDB-T-2 F and PBDB-T-2 Cl) and acceptors(IT-4 F and IT-4 Cl). The results suggest that all the OSCs based on different donor and acceptor combinations can deliver good PCEs around 13%–14%. Chlorination is more effective than fluorination in downshifting the molecular energy levels and broadening the absorption spectra. The influence of chlorination and fluorination on the crystallinity of the resulting materials is dependent on their introduction positions. As chlorination has the advantage of easy synthesis, it is more attractive in designing low-cost photovoltaic materials and therefore may have more potential in largescale applications. | Yun Zhang Huifeng Yao Shaoqing Zhang Yunpeng Qin Jianqi Zhang Liyan Yang Wanning Li Zhixiang Wei Feng Gao Jianhui Hou | 2018 | Science China Chemistry2018，61，10： | 11 |
| 12 | Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances显示文摘Lactic acid bacteria(LAB)are a representative probiotic.As the dominant flora in the human intestinal tract,LAB can regulate the balance of human intestinal flora and improve host health.The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population,with excellent probiotics and high antibacterial activity.After 16S ribosomal RNA(rRNA)homology and phylogenetic tree analysis,potential probiotics were tested for their antibacterial activity,resistance to artificial gastrointestinal fluid and drugs,surface hydrophobicity,and safety.Three strains of LAB with acid resistance,bile salt resistance,epithelial cell adhesion,and no multidrug resistance were selected:Lactobacillus salivarius,Leuconostoc lactis,and Lactobacillus paracasei.Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L.lactis were organic acids,whereas those of L.salivarius and L.paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity.These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens. | Zhijing LIU Cong XU Ran TIAN Wan WANG Jiage MA Liya GU Fei LIU Zhanmei JIANG Juncai HOU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，7： | 10 |
| 13 | Aluminium toxicity targets PIN2 in Arabidopsis root apices: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport显示文摘The most obvious symptom of Al toxicity is the inhibition of root growth. However, the mechanism of Al-inhibiting root growth remains to be elucidated. In this study, auxin transport and vesicle movement of an auxin-efflux carrier (PIN2) were investigated in Arabidopsis roots in response to Al stress. Results indicated that Al inhibited the apical transport of auxin in root tips of Arabidopsis significantly. The severe inhibition was localized in the cells of transition zone, where the concentration of auxin was only 34% that of the control. Brefeldin A (BFA), an inhibitor of vesicle transport, induced the dot-like structure of PIN2 vesicle significantly. Al decreased the size of dot-like structure of PIN2 vesicles. Re- sults of real-time RT-PCR and Western-blotting analysis showed that Al increased the transcript level of PIN2 and the accumulation of PIN2 protein in horizontal direction of plasma membrane, but decreased its distribution in endosomes, suggesting that Al inhibited the transport of PIN2 vesicles from plasma membrane to endosomes. Results of cytoskeleton-depolymering drugs indicated that it was via the pathway of disruption of actin microfilaments that Al inhibited the transport of PIN2 vesicles. Exposed to Al stress, the cells of elongation zone had less Al uptake and less transport frequency of vesicles than cells of transition zone. Taken together, our results suggested that Al inhibited root growth mainly by modulating the transport of PIN2 vesicles between plasma membrane and endosomes, thus block- ing auxin transport and root growth. | SHEN Hong HOU NingYan Markus SCHLICHT WAN YingLang Stefano MANCUSO Frantisek BALUSKA | 2008 | Chinese Science Bulletin2008，53，16： | 9 |
| 14 | A chlorinated low-bandgap small-molecule acceptor for organic solar cells with 14.1% efficiency and low energy loss显示文摘A new acceptor-donor-acceptor(A-D-A) type small-molecule acceptor NCBDT-4 Cl using chlorinated end groups is reported.This new-designed molecule demonstrates wide and efficient absorption ability in the range of 600–900 nm with a narrow optical bandgap of 1.40 eV. The device based on PBDB-T-SF:NCBDT-4 Cl shows a power conversion efficiency(PCE) of 13.1%without any post-treatment, which represents the best result for all as-cast organic solar cells(OSCs) to date. After device optimizations, the PCE was further enhanced to over 14% with a high short-circuit current density(Jsc) of 22.35 m A cm-2 and a fill-factor(FF) of 74.3%. The improved performance was attributed to the more efficient photo-electron conversion process in the optimal device. To our knowledge, this outstanding efficiency of 14.1% with an energy loss as low as 0.55 eV is among the best results for all single-junction OSCs. | Bin Kan Huanran Feng Huifeng Yao Meijia Chang Xiangjian Wan Chenxi Li Jianhui Hou Yongsheng Chen | 2018 | Science China Chemistry2018，61，10： | 9 |
| 15 | Constraints on the Archean atmospheric oxygen and sulfur cycle from mass-independent sulfur records from Anshan-Benxi BIFs, Liaoning Province, China显示文摘The Archean atmospheric oxygen concentration and sulfur cycle was long debated. The banded iron formation (BIF) is a special type of the sedimentary formation, which has truly recorded the atmospheric and oceanic conditions at that time. In this study, the composition of multiple sulfur isotope (δ 34S/δ 33S/δ 32S) for sulfides bedded in the Archean (~2.7 Ga) BIFs, in Anshan-Benxi area of Liaoning Province has been measured. The value of △33S varies from -0.89‰ to +1.21‰, which shows very obvious mass-independent fractionation (MIF) signatures. These non-zero △33S values indicate that the Archean sulfur cycles are different from what it is today, which have been deeply influenced by gas phase photochemical reactions. Algoma-type BIFs which are closely related to the volcanic activity have negative △33S value, however, Superior-type BIFs which are far away from the volcanic center have positive △33S value. The δ 34S varies in a large range from -22.0‰ to +11.8‰, which indicates that the bacteria reduction activity has already existed at that time, and that the oceanic sulfate concentration has at least reached 1 mmol/L in local areas. Combined with the contemporaneous existence of the hematite, magnetite and the occurrence and preservation of the sulfur MIF, it can be inferred that the Archean atmospheric oxygen level must be at 10-2―10-3 of the present atmospheric level (PAL). | HOU KeJun1,2, LI YanHe1,2? & WAN DeFang1,2 1 Key Laboratory of Metallogeny and Mineral Resource Assessment, Institute of Mineral Resource, Chinese Academy of Geological Sciences (CAGS), Beijing 100037, China 2 Key Laboratory of Isotope Geology, CAGS, Beijing 100037, China | 2007 | Science China Earth Sciences2007，50，10： | 9 |
| 16 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 17 | A Novel Thixotropic Phenomenon——Complex Thixotropic Behavior显示文摘ANovelThixotropicPhenomenon——ComplexThixotropicBehavior*Towhomcorrespondenceshouldbeaddresed.HOUWan-guo*,SUNDe-jun,HANShu-hua... | HOU Wan guo, SUN De jun, HAN Shu hua, ZHANG Chun guang and WANG Guo ting (Institute of Colloid and Interface Chemistry, Shandong University , Jinan, 250100) | 1997 | Chemical Research in Chinese Universities1997，13，1： | 8 |
| 18 | Propofol inhibits inflammation and lipid peroxidation following cerebral ischemia/reperfusion in rabbits显示文摘The present study established a rabbit model of global cerebral ischemia using the 'six-vessel' method,which was reperfused after 30 minutes of ischemia.Rabbits received intravenous injection of propofol at 5 mg/kg prior to ischemia and 20 mg/kg per hour after ischemia until samples were prepared.Results revealed that propofol inhibited serum interleukin-8,endothelin-1 and malondialdehyde increases and promoted plasma superoxide dismutase activity after cerebral ischemia/reperfusion.In addition,cerebral cortex edema was attenuated with little neuronal nuclear degeneration and pyknosis with propofol treatment.The cross-sectional area of neuronal nuclei was,however,increased following propofol treatment.These findings suggested that propofol could improve anti-oxidant activity and inhibit synthesis of inflammatory factors to exert a protective effect on cerebral ischemia/reperfusion injury. | Xiaodong Wei Xing Wan Bo Zhao Jiabao Hou Min Liu Bangchang Cheng | 2012 | Neural Regeneration Research2012，7，11： | 8 |
| 19 | Provenance of the Paleoproterozoic Hutuo Group basal conglomerates and Neoarchean crustal growth in the Wutai Mountains,North China Craton:Evidence from granite and quartzite pebble zircon U-Pb ages and Hf isotopes显示文摘Zircon U-Pb ages(SHRIMP and LA-ICPMS) and Lu-Hf isotope data(LA-ICPMS) are presented for two granite and two quartzite pebbles from the basal conglomerates of the Sijizhuang Formation in the Hutuo Group from the Wutai Mountains area in the North China Craton.These two granite pebbles give zircon 207 Pb/206 Pb ages of 2513±8 Ma and 2527±8 Ma respectively,which are consistent with the emplacement ages of the Wangjiahui grey granite and Guangmingsi or Shifo granite in the Wutai Mountains.Detrital zircons from those two quartzite pebbles are mostly 2550-2490 Ma old with lesser number of 2800-2550 Ma grains,which is similar to the ages of detrital zircons from quartzites in the Gaofan Subgroup of the Neoarchean Wutai Group.Thus,the pebbles in the Hutuo Group basal conglomerates were derived locally from Wutai Mountains Neoarchean sources.Zircons from the Sijizhuang Formation conglomerate granite and quartzite pebbles mostly have positive ε Hf(t) values,a minority with ε Hf(t) values like model depleted mantle(DM) of the same age,but with most showing DM model ages 200-100 Ma.This indicates that most of the source materials were derived from the mantle within the previous 200 million years,whereas some are derived from 2550-2510 Ma juvenile crustal additions.This additional evidence suggests that in the North China Craton there was important initial polycyclic crustal formation and cratonization in the late Neoarchaean,prior to superimposed Palaeoproterozoic orogenic cycles. | DU LiLin YANG ChongHui WANG Wei REN LiuDong WAN YuSheng SONG HuiXia GAO LinZhi GENG YuanSheng HOU KeJun | 2012 | Science China Earth Sciences2012，55，11： | 8 |
| 20 | Genome analysis and signature discovery for diving and sensory properties of the endangered Chinese alligator显示文摘Crocodilians 正在潜水能为时间的长时期在水下面屏息并且是有优秀感觉能力的黄昏的动物的爬行动物。他们包括鸟的一个姐妹系并且没有性染色体。这里,我们报导危及的中国短吻鳄(短吻鳄 sinensis ) 的染色体顺序并且描述它的唯一的特征。下一代的定序产生了未加工的顺序的 314 Gb,产出 2.3 Gb 的一种染色体尺寸。22 200 基因的一个总数用 de novo 在短吻鳄 sinensis 被预言,基于相同、基于 RNA 的联合模型。长潜水的行为的基因基础包括酸式碳酸盐绑定血红素基因,磷酸盐绑定和特殊酸式碳酸盐绑定氧搬运的常规的 co-functioning,和断然选择的精力的复制新陈代谢,铵酸式碳酸盐排泄和心肌收缩。进一步,我们阐明了柔韧的短吻鳄 sinensis 感觉系统包括一个显著地扩展的嗅觉的受体全部剧目,很快演变神经相关的细胞的部件和视觉感觉,和夜里的积极选择视觉相关的视蛋白和健全联系察觉的 otopetrin。我们也为抗菌剂肽的天生的免疫和表示为分成三部分的包含主题的 C 类型 lectin 和 butyrophilin 基因的适应免疫以及扩大与系特定的抗原演讲基因的一个可观的数字发现了一个开发得好的免疫系统。在 situ 杂交的 Multifluorescence 显示出那个短吻鳄染色体 3,它编码 DMRT1,与鸡染色体 Z 一起展出重要 synteny。最后,人口历史分析百万年以前显示了人口混合 0.60-1.05,当 Qinghai 西藏的高原被高举时。 | Qiu-Hong Wan Sheng-Kai Pan Li Hu Ying Zhu Peng-Wei Xu Jin-Quan Xia Hui Chen Gen-Yun He Jing He Xiao-Wei Ni Hao-Long Hou Sheng-Guang Liao Hai-Qiong Yang Ying Chen Shu-Kun Gao Yun-Fa Ge Chang-Chang Cao Peng-Fei Li Li-Ming Fang Li Liao Shu Zhang Meng-Zhen Wang Wei Dong Sheng-Guo Fang | 2013 | Cell Research2013，23，9： | 8 |
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