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| 1 | Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules显示文摘由病毒的 transduction 的四抄写因素 Oct4, Klf4, Sox2 和 c-Myc 的介绍能导致体的房间的 reprogramming 进导致的 pluripotent 干细胞(iPSCs ) ,但是 iPSCs 的使用被病毒的交货系统的使用妨碍。导致化学药品的 reprogramming 把一条新奇途径提供给没有任何病毒的基于向量的基因修正,产生 iPSCs。尽管,以前的报告证明几个小分子能代替一些 reprogramming 因素至少二个抄写因素, Oct4 和 Klf4,仍然被要求从老鼠产生 iPSCs 胚胎的成纤维细胞。这里,我们识别特定的化学联合,它是足够的面对一个单个抄写因素从胚胎的老鼠和成年成纤维细胞允许 reprogramming, Oct4 在 20 天以内,代替 Sox2, Klf4 和 c-Myc。用这个处理产生的 iPSCs 类似于胚胎的干细胞以全球基因表示介绍的老鼠,在 vitro 并且在 vivo 的 epigenetic 地位和 pluripotency。我们也发现那 8 天 Oct4 正式就职是足够的面对小分子启用导致 Oct4 的 reprogramming,它建议 reprogramming 在开始的 8 天以内被开始并且独立于连续外长的 Oct4 表示。没有基因修正,这些发现将帮助 iPSCs 的未来产生,以及阐明位于 reprogramming 过程下面的分子的机制。 | Yanqin Li Qiang Zhang Xiaolei Yin Weifeng Yang Yuanyuan Du Pingping Hou Jian Ge Chun Liu Weiqi Zhang Xu Zhang Yetao Wu Honggang Li Kang Liu Chen Wu Zhihua Song Yang Zhao Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，1： | 51 |
| 2 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 3 | Cardiomyocyte overexpression of miR-27b induces cardiac hypertrophy and dysfunction in mice显示文摘最近的研究开始在心脏的肥大和机能障碍的致病揭示了 microRNAs (miRNAs ) 的关键角色。在这研究,我们测试了是否转变生长 factor-β(TGF-β) 调整 miRNA 在心脏的肥大和心失败(HF ) 的发展起了一个枢轴的作用。我们观察到 miR-27b 是在 cardiomyocyte 特定的 Smad4 猛烈老鼠的心的 upregulated,它开发了心脏的肥大。在 vitro,实验证明 miR-27b 表示能被 TGF-β 禁止; 1 并且它的 overexpression 支持了 hypertrophic 房间生长,当 miR-27b 抑制导致了 hypertrophic 房间的抑制时,生长由 phenylephrine (PE ) 引起了处理。而且,有 miR-27b 的 cardiomyocyte 特定的 overexpression 的转基因的老鼠的分析表明 miR-27b overexpression 是足够的导致心脏的肥大和机能障碍。我们验证了 peroxisome 激活 proliferator 的 receptor-γ(PPAR-γ) 作为在 cardiomyocyte 的 miR-27b 的一个直接目标。一致地, miR-27b 转基因的老鼠显著地显示了 PPAR-γ 的底层;比控制鼠标。而且,在用在一只 pressure-overload-induced 老鼠的特定的 antagomir 的 miR-27b 的 vivo silencing, HF 的模型增加了心脏的 PPAR-γ表示,稀释心脏的肥大和机能障碍。我们的学习的结果表明那 TGF-β 1-regulated miR-27b 涉及心脏的肥大的规定,并且为心脏病作为一个有效治疗学的目标验证 miR-27b。 | Jian Wang Yao Song Yan Zhang Han Xiao Qiang Sun Ning Hou Shuilong Guo Youliang Wang Kaiji Fan Dawei Zhan Lagabaiyila Zha Yang Cao Zhenhua Li Xuan Cheng Youyi Zhang Xiao Yang | 2012 | Cell Research2012，22，3： | 36 |
| 4 | Analyzing the Flavor Compounds in Chinese Traditional Fermented Shrimp Pastes by HS-SPME-GC/MS and Electronic Nose显示文摘Shrimp paste is a type of condiments with high nutritional value.However,the flavors of shrimp paste,particularly the non-uniformity flavors,have limited its application in food processing.In order to identify the characteristic flavor compounds in Chinese traditional shrimp pastes,five kinds of typical commercial products were evaluated in this study.The differences in the volatile composition of the five products were investigated.Solid phase micro-extraction method was employed to extract the volatile compounds.GC-MS and electronic nose were applied to identify the compounds,and the data were analyzed using principal component analysis(PCA).A total of 62 volatile compounds were identified,including 8 alcohols,7 aldehydes,3 ketones,7 ethers,7 acids,3 esters,6 hydrocarbons,12 pyrazines,2 phenols,and 7 other compounds.The typical volatile compounds contributing to the flavor of shrimp paste were found as follows:dimethyl disulfide,dimethyl tetrasulfide,dimethyl trisulfide,2,3,5-trimethyl-6-ethyl pyrazine,ethyl-2,5-dimethyl-pyrazine,phenol and indole.Propanoic acid,butanoic acid,furans,and 2-hydroxy-3-pentanone caused unpleasant odors,such as pungent and rancid odors.Principal component analysis showed that the content of volatile compounds varied depending on the processing conditions and shrimp species.These results indicated that the combinations of multiple analysis and identification methods could make up the limitations of a single method,enhance the accuracy of identification,and provide useful information for sensory research and product development. | FAN Yan YIN Li-ang XUE Yong LI Zhaojie HOU Hu XUE Changhu | 2017 | Journal of Ocean University of China2017，16，2： | 25 |
| 5 | Different dose combinations of bortezomib and dexamethasone in the treatment of relapsed or refractory myeloma: an open-label,observational, multi-center study in China显示文摘背景尽管以前的临床的学习表明与 dexamethasone 相结合的 bortezomib 改进了 relapsed 或倔强的多重骨髓瘤(RRMM ) 的结果, bortezomib 和 dexamethasone 的最佳的剂量联合仍然保持未知。这试用试图与 relapsed 在 168 个病人全部的 China.Methods A 在 RRMM 病人的治疗观察 bortezomib 和 dexamethasone 的不同剂量联合的功效和安全多重骨髓瘤(公里)对倔强在以免二优先的治疗在这 multicenter 被注册,开标签,非使随机化,未来的临床的试用。二十个病人为多达 8 个周期和口头或静脉内的 dexamethasone 收到了为 2 星期一个 3 星期的周期两次每周的 bortezomib 的 1.3 mg/m2 在日子并且在每 bortezomib 剂量(组 1 ) 以后的 20 mg;66 个病人收到了 bortezomib 和 dexamethasone 的不到 1.3 mg/m2 (0.7-1.0 mg/m2 ) 一样的时间表(组 2 ) 上的 20 mg;37 个病人收到了 40 mg (组 3 ) 和 45 个病人收到了的 bortezomib 和 dexamethasone 的 1.3 mg/m2 bortezomib 和 dexamethasone 的不到 1.3 mg/m2 (0.7-1.0 mg/m2 ) 40 mg (组 4 ) 。反应为血和髓移植根据欧洲组的标准被评估并且由一个独立评论委员会证实了。不利事件根据国家癌症研究所被分级普通毒性标准,版本 3.0。结果组的中部的年龄 1 ~ 4 分别地是 61,62, 56,和 60 年。大多数病人在公里的阶段/,最普通的子类型是对组 1~4 的 bortezomib 和 dexamethasone 的全面反应的率是的 IgG 72.2 ( 13/18 ), 73.8 ( 48/65 ), 78.8 ( 26/33 )并且 78.0 ( 32/41 )( P=0.91 ),包括 22.2 的完全的反应率( 4/18 ), 20.0 ( 13/65 ), 33.3 ( 11/33 )并且 29.3 ( 12/41 )( P=0.67 )分别地。在这 4 个组(P 0.05 ) 之中及时没有统计意义到前进和全面幸存。在全部 4 个组的任何等级的最通常不利的事件是疲劳,胃肠的效果,外部神经病和 thrombocytopenia ,并且除了外部神经病在组 3 更经常被报导之外,在在 4 个组( P 0.05 )之中的不利事件的数字没有意义( 36.3 )比在组 2 ( 13.8 , P 0.05 )并且组织 4 ( 14.6 , PO.05 ) .Conclusions bortezomib 和 dexamethasone 的联合在中国 RRMM 病人与高回答被联系。功效的重要差别都没在 bortezomib 和 dexamethasone 的不同剂量联合被检测。而且,没有在中国与 RRMM 在病人的治疗影响结果, bortezomib 的低剂量减少了外部神经病的发生。 | YUAN Zhen-gang JIN Jie HUANG Xiao-jun LI Yan CHEN Wen-ming LIU Zhuo-gang CHEN Xie-qun SHEN Zhi-xiang HOU Jian | 2011 | Chinese Medical Journal2011，，19： | 24 |
| 6 | Effects of mycophenolate mofetil,valsartan and their combined therapy on preventing podocyte loss in early stage of diabetic nephropathy in rats显示文摘背景 Podocyte 在糖尿病的 nephropathy (DN ) 的发展有煽动性的角色。Mycophenolate mofetil (MMF ) ,一个反煽动性的代理人,能压制巨噬细胞渗入并且在导致 streptozotocin 的糖尿病的老鼠减少肾的损害。血管收缩素受体 blocker (ARB ) ,另一个肾的保护代理人,在 DN 的罐头减少 podocyte 损失。在这研究,我们检测了单核白血球 chemoattractant protein-1 ( MCP-1 )和 nephrin 的表示层次独自并且在有 valsartan 的联合在 DN 在煽动性的反应评估 podocyte 的角色,观察并且比较 MMF 的效果,在在 streptozotocin ( STZ )阻止 podocyte 损失以后,导致的糖尿病的 rats.Methods 糖尿病患者模型被 STZ ( 65 mg/kg )的单个腹注射在 uninephrectomized 男性 Wistar 老鼠构造。成功地导致的糖尿病的老鼠随机被划分成四个组:没有处理组(DM ) 的糖尿病, valsartan 对待组(DMV ) , MMF 对待组(DMM ) ,并且联合了治疗组(DMVM ) 。一样的兄弟的正常老鼠被选择为控制(NC ) 。在第 8 星期的结束,浆液生物化学, 24 小时的尿蛋白质(起来) 并且肾重量 / 身体重量(RWK/B ) 的比率被测量。老鼠通过光和电子显微镜为肾的 histomorphology 的观察被牺牲。Nephrin, desmin 和 MCP-1 层次被半量的 immunohistochemical 试金检测。实时量的 PCR 被用来与组 NC 相比检测 nephrin 和 MCP-1.Results 的 mRNA 层次,浆液葡萄糖水平,在上面 24 小时并且在组 DM 的 RWK/B 显著地更高(P0.01 ) ,并且在 DM 组的 nephrin mRNA 水平显著地更低(P0.05 ) 。在组 DMV, DMM 和 DMVM 的 nephrin mRNA 表示层次都比 DM 组(P0.05 ) 的高,没有重要差别在三个处理组(P0.05 ) 之中被发现。有 MMF, valsartan 或他们的联合的处理能显著地减少在上面 24 小时并且 RWK/B,并且在糖尿病的老鼠压制 glomerulosclerosis 和空隙的 fibrotic 损害。在糖尿病的老鼠,在肾的 desmin 和 MCP-1 的高表情被 valsartan, MMF 或他们的 combination.Conclusions 压制 Podocytes 涉及糖尿病的老鼠的煽动性的反应。MMF 能压制 MCP-1 和 desmin 表示,提高 nephrin 表示,并且稀释在糖尿病的老鼠的 proteinuria。valsartan 和 MMF 的联合治疗没在肾的保护上在 monotherapies 上显示出任何优势。MMF 可以通过阻止 podocytes 损失和反煽动性的活动在糖尿病的 nephropathy 的早阶段有 renoprotective 效果。 | ZHANG Yan CHEN Bing HOU Xiang-hua GUAN Guang-ju LIU Gang LIU Hai-ying LI Xue-gang | 2007 | Chinese Medical Journal2007，，11： | 23 |
| 7 | Severity of sepsisis is correlated with the elevation of serum high-mobility group box 1 in rats显示文摘背景败血是以特别护理单位的死亡的一个领先的原因。晚煽动性的 cytokine,高活动性的组盒子 1 (HMGB1 ) ,在败血起一个关键作用。在现在的学习,我们在 rats.Methods 在 导致lipopolysaccharide 的败血调查了在浆液 HMGB1 层次和器官损害的严厉之间的协会与器官损害的不同的度生产败血的一个动物模型,动物与 lipopolysaccharide 的三不同剂量被对待( 4 , 8 和 16 mg/kg ),并且在控制组的动物与车辆的一样的体积被对待(盐)。浆液 HMGB1 的层次在 lipopolysaccharide ( LPS )或车辆注射以后在 0 , 2 , 4 , 8 , 16 , 24 , 32 和 48 个小时被测量,同时为机关损害的严厉的生物化学、组织病理学说的指示物是 HMGB1 的水平有的 assessed.Results 一积极,与浆液的反常变化的高关联心脏的 troponin ,丙氨酸 aminotransferase , aspartate aminotransferase , creatinine 和血脲氮,以及心,肺,肝和 kidney.Co | HOU Li-chao QIN Ming-zhe ZHENG Li-na LU Yan WANG Qiang PENG Dao-rong YU Xin-ping XIN Yu-chang JI Gen-lin XIONG Li-ze | 2009 | Chinese Medical Journal2009，，4： | 22 |
| 8 | Number concentration and size distributions of submicron particles in Jinan urban area:Characteristics in summer and winter显示文摘喷雾器数字集中和尺寸分发在 Jinan 城市的城市的地点在 2006 的夏天和冬季与最新发达的宽范围的粒子分光计被测量。这里报导了不同观察季节的好粒子的特征。为在 10 500 nm 的直径范围的粒子的相对高数字集中在两个季节被观察。比在所有空气粒子的数字集中上的 100 nm 和百分比比在世界上在另外的城市的地点被测量的高是大部分,主导的数字才在小的颗粒直径散布了。在夏天的数字平均数直径比在冬季小得多,强烈在不同季节建议极其细小的粒子的不同起源。也就是说,在极其细小的模式的粒子主要在夏天来自成核和新粒子形成当时从在冬季的交通排放。日报变化也支持了这个点。在 10 200 nm 的直径范围的数字集中在中午得到了他们的山峰价值,很好在夏天与 SO2 的混合比率和太阳辐射的紧张相关。当时在冬季,那些在交通小时期间在一样的直径范围显示出主要山峰发生在早上和晚上。 | GAO Jian WANG Jin CHENG Shu-hui XUE Li-kun YAN Huai-zhong HOU Lu-jian JIANG Yu-quan WANG Wen-xing | 2007 | Journal of Environmental Sciences2007，19，12： | 21 |
| 9 | Platelets in hemostasis and thrombosis:Novel mechanisms of fibrinogen-independent platelet aggregation and fibronectin-mediated protein wave of hemostasis显示文摘Platelets are small anucleate cells generated from megakaryocytes in the bone marrow.Although platelet generation,maturation,and clearance are still not fully understood,significant progress has been made in the last 1-2 decades.In blood circulation,platelets can quickly adhere and aggregate at sites of vascular injury,forming the platelet plug(i.e.the first wave of hemostasis).Activated platelets can also provide negatively charged phosphatidylserinerich membrane surface that enhances cell-based thrombin generation,which facilitates blood coagulation(i.e.the second wave of hemostasis).Platelets therefore play central roles in hemostasis.However,the same process of hemostasis may also cause thrombosis and vessel occlusion,which are the most common mechanisms leading to heart attack and stroke following ruptured atherosclerotic lesions.In this review,we will introduce the classical mechanisms and newly discovered pathways of platelets in hemostasis and thrombosis,including fibrinogen-independent platelet aggregation and thrombosis,and the plasma fibronectin-mediated 'protein wave' of hemostasis that precedes the classical first wave of hemostasis.Furthermore,we briefly discuss the roles of platelets in inflammation and atherosclerosis and the potential strategies to control atherothrombosis. | Yan Hou Naadiya Carrim Yiming Wang Reid C.Gallant Alexandra Marshall Heyu Ni | 2015 | The Journal of Biomedical Research2015，29，6： | 21 |
| 10 | Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity显示文摘感觉神经原被不同发信号的网络和接受特征区分。因此,感觉神经原类型能被连接基于 transcriptome 的神经原与感觉显型打字定义。这里,我们分类老鼠的 somatosensory 神经原由高范围的单个房间的定序 RNA 的背面的根中心(DRG )(10 950 ±1 218 基因每神经原) 并且神经原基于尺寸的层次聚类。而且,对皮肤的刺激作出回应的单个 DRG 神经原被记录用一在里面 vivo 整个房间的补丁夹钳技术并且由神经原类型分类基因标记。小直径 DRG 神经原被分类进低阀值的 mechanoreceptor 的一种类型和 mechanoheat nociceptors (MHN ) 的五种类型。每 MHN 类型进一步被分成二种子类型。大 DRG 神经原被分成四种类型,包括表示联系 BAI1 的蛋白质 2-like 的 neurexophilin 1 快车 MHN 和机械 nociceptors (MN ) 1 (Baiap2l1 ) 。表示 trafficking 蛋白质粒子建筑群 3-like 和 Baiap2l1 显著的 MN 的 Mechanoreceptors 各被细分进二种子类型。这些结果为编目 somatosensory 神经原和他们的 transcriptome 数据库提供一个新系统。 | Chang-Lin Li Kai-Cheng Li Dan Wu Yan Chen Hao Luo Jing-Rong Zhao Sa-Shuang Wang Ming-Ming Sun Ying-Jin Lu Yan-Qing Zhong Xu-Ye Hu Rui Hou Bei-Bei Zhou Lan Bao Hua-Sheng Xiao Xu Zhang | 2016 | Cell Research2016，26，1： | 21 |
| 11 | Lp-PLA2 inhibition prevents Ang Ⅱ-induced cardiac inflammation and fibrosis by blocking macrophage NLRP3 inflammasome activation显示文摘Macrophage-mediated inflammation plays an important role in hypertensive cardiac remodeling,whereas effective pharmacological treatments targeting cardiac inflammation remain unclear.Lipoprotein-associated phospholipase A2(Lp-PLA2)contributes to vascular inflammation-related diseases by mediating macrophage migration and activation.Darapladib,the most advanced Lp-PLA2 inhibitor,has been evaluated in phase Ⅲ trials in atherosclerosis patients.However,the role of darapladib in inhibiting hypertensive cardiac fibrosis remains unknown.Using a murine angiotensin Ⅱ(Ang Ⅱ)infusion-induced hypertension model,we found that Pla2g7(the gene of Lp-PLA2)was the only upregulated PLA2 gene detected in hypertensive cardiac tissue,and it was primarily localized in heart-infiltrating macrophages.As expected,darapladib significantly prevented Ang Ⅱ-induced cardiac fibrosis,ventricular hypertrophy,and cardiac dysfunction,with potent abatement of macrophage infiltration and inflammatory response.RNA sequencing revealed that darapladib strongly downregulated the expression of genes and signaling pathways related to inflammation,extracellular matrix,and proliferation.Moreover,darapladib substantially reduced the Ang Ⅱ infusion-induced expression of nucleotide-binding oligomerization domain-like receptor with pyrin domain 3(NLRP3)and interleukin(IL)-1β and markedly attenuated caspase-1 activation in cardiac tissues.Furthermore,darapladib ameliorated Ang Ⅱ-stimulated macrophage migration and IL-1β secretion in macrophages by blocking NLRP3 inflammasome activation.Darapladib also effectively blocked macrophage-mediated transformation of fibroblasts into myofibroblasts by inhibiting the activation of the NLRP3 inflammasome in macrophages.Overall,our study identifies a novel anti-inflammatory and anti-cardiac fibrosis role of darapladib in Lp-PLA2 inhibition,elucidating the protective effects of suppressing NLRP3 inflammasome activation.Lp-PLA2 inhibition by darapladib represents a novel therapeutic strategy for hypertensive cardiac damage treatment. | Si-lin Lv Zi-fan Zeng Wen-qiang Gan Wei-qi Wang Tie-gang Li Yu-fang Hou Zheng Yan Ri-xin Zhang Min Yang | 2021 | Acta Pharmacologica Sinica2021，42，12： | 21 |
| 12 | Neuroprotective effects of rutaecarpine on cerebral ischemia reperfusion injury显示文摘Rutaecarpine,an active component of the traditional Chinese medicine Tetradium ruticarpum,has been shown to improve myocardial ischemia reperfusion injury.Because both cardiovascular and cerebrovascular diseases are forms of ischemic vascular disease,they are closely related.We hypothesized that rutaecarpine also has neuroprotective effects on cerebral ischemia reperfusion injury.A cerebral ischemia reperfusion model was established after 84,252 and 504 μg/kg rutaecarpine were given to mice via intraperitoneal injection,daily for 7 days.Results of the step through test,2,3,5-triphenyl tetrazolium chloride dyeing and oxidative stress indicators showed that rutaecarpine could improve learning and memory ability,neurological symptoms and reduce infarction volume and cerebral water content in mice with cerebral ischemia reperfusion injury.Rutaecarpine could significantly decrease the malondialdehyde content and increase the activities of superoxide dismutase and glutathione peroxidase in mouse brain.Therefore,rutaecarpine could improve neurological function following injury induced by cerebral ischemia reperfusion,and the mechanism of this improvement may be associated with oxidative stress.These results verify that rutaecarpine has neuroprotective effects on cerebral ischemia reperfusion in mice. | Chunlin Yan Ji Zhang Shu Wang Guiping Xue Yong Hou | 2013 | Neural Regeneration Research2013，8，22： | 20 |
| 13 | Analysis and Control of S/SP Compensation Contactless Resonant Converters显示文摘非接触谐振变换器的补偿方式直接影响到系统的增益特性和控制特性,是非接触能量传输技术的研究重点之一。考虑到非接触应用场合中的变参数特性,论文提出了一种可适应负载及变压器参数变化的新型的串/串并(series/seriesparallel,S/SP)补偿拓扑。该补偿拓扑具有增益交点处输入相角为零,且增益交点值固定不随变压器耦合系数而改变的优点。论文对该新型补偿拓扑进行了特性分析;并针对其增益交点处输入相角为零以及增益交点处增益曲线相对平坦的优点,提出锁相环(phaselockloop,PLL)控制和恒频控制2种控制策略。采用所提出的补偿方式,设计了一台PLL控制60W的谐振变换器和一台1.5kW恒频控制的原理样机。实验结果与理论分析一致,验证了所提出的新型补偿方式的优点。1.5kW的样机在10cm气隙满载条件下效率达到95.2%。 | HOU Jia CHEN Qianhong YAN Kaiqin LI Mingshuo ZHANG Qiang RUAN Xinbo | 2013 | 中国电机工程学报2013，33，33： | 19 |
| 14 | Application of an acrylic vessel supported by a stainless-steel truss for the JUNO central detector显示文摘在 Daya 海湾实验的成功以后, Jiangmen 地下的中微子天文台(Jupiter 之妻) 被发射测量中微子团层次和摆动参数并且学习另外的中微子物理。它的中央察觉者从反应堆,地球,空气,和阳光被放为反中微子。中央察觉者的主要要求是液体闪烁器的 20 kt 的抑制,作为目标团,和 3% 精力分辨率。它关于有它的内部表面上的 PMT 的 75% 范围的 38.5 m 的一个球形状察觉者。因为它必须为物理测量的几种不同类型满足要求并且在它的结构和工程拥有可行性和可靠性,如此的一个巨大的察觉者的设计是大挑战,都在合理时间和费用。为 JUNO 中央察觉者的一种选择是超丙烯酸的球在水里沉没了防护背景。这份报纸为如此的一个丙烯酸的球建议一个结构的计划那被支持由一不锈钢捆绑,在土木工程由支持点的眼镜窗帘墙启发了。计划的初步的设计被有限元素(FE ) 用 ABAQUS 完成并且验证方法。FE 分析证明计划能在 5 ~ 10 MPa 的限制以内控制丙烯酸的球的压力水平,根据中央察觉者的设计目的需求。计划具有突出的全球稳定性并且在本地连接上允许各种各样的选择。我们证明计划可行性好并且应该是为中央察觉者的一种合理选择。 | WANG Yuan Qing ZONG Liang HENG Yue Kun WANG Zong Yi ZHOU Yan HOU Shao Jing QIN Zhong Hua MA Xiao Yan | 2014 | Science China(Technological Sciences)2014，57，12： | 19 |
| 15 | Modified constraint-induced movement therapy alters synaptic plasticity of rat contralateral hippocampus following middle cerebral artery occlusion显示文摘Modified constraint-induced movement therapy is an effective treatment for neurological and motor impairments in patients with stroke by increasing the use of their affected limb and limiting the contralateral limb.However,the molecular mechanism underlying its efficacy remains unclear.In this study,a middle cerebral artery occlusion(MCAO)rat model was produced by the suture method.Rats received modified constraint-induced movement therapy 1 hour a day for 14 consecutive days,starting from the 7^th day after middle cerebral artery occlusion.Day 1 of treatment lasted for 10 minutes at 2r/min,day 2 for 20 minutes at 2 r/min,and from day 3 onward for 20 minutes at 4 r/min.CatWalk gait analysis,adhesive removal test,and Y-maze test were used to investigate motor function,sensory function as well as cognitive function in rodent animals from the 1st day before MCAO to the 21^st day after MCAO.On the 21^st day after MCAO,the neurotransmitter receptor-related genes from both contralateral and ipsilateral hippocampi were tested by micro-array and then verified by western blot assay.The glutamate related receptor was shown by transmission electron microscopy and the glutamate content was determined by high-performance liquid chromatography.The results of behavior tests showed that modified constraint-induced movement therapy promoted motor and sensory functional recovery in the middle cerebral artery-occluded rats,but had no effect on cognitive function.The modified constraint-induced movement therapy upregulated the expression of glutamate ionotropic receptor AMPA type subunit 3(Gria3)in the hippocampus and downregulated the expression of the beta3-adrenergic receptor gene Adrb3 and arginine vasopressin receptor 1 A,Avprla in the middle cerebral artery-occluded rats.In the ipsilateral hippocampus,only Adra2 a was downregulated,and there was no significant change in Gria3.Transmission electron microscopy revealed a denser distribution the more distribution of postsynaptic glutamate receptor 2/3,which is an a-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor,within 240 nm of the postsynaptic density in the contralateral cornu ammonis 3 region.The size and distribution of the synaptic vesicles within 100 nm of the presynaptic active zone were unchanged.Western blot analysis showed that modified constraint-induced movement therapy also increased the expression of glutamate receptor 2/3 and brain-derived neurotrophic factor in the hippocampus of rats with middle cerebral artery occlusion,but had no effect on Synapsin I levels.Besides,we also found modified constraint-induced movement therapy effectively reduced glutamate content in the contralateral hippocampus.This study demonstrated that modified constraint-induced movement therapy is an effective rehabilitation therapy in middle cerebral artery-occluded rats,and suggests that these positive effects occur via the upregulation of the postsynaptic membrane a-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor expression.This study was approved by the Institutional Animal Care and Use Committee of Fudan University,China(approval No.201802173 S)on March 3,2018. | Bei-Yao Gao Dong-Sheng Xu Pei-Le Liu Ce Li Liang Du Yan Hua Jian Hu Jia-Yun Hou Yu-Long Bai | 2020 | Neural Regeneration Research2020，15，6： | 19 |
| 16 | Pegylated interferon α-2b up-regulates specific CD8+ T cells in patients with chronic hepatitis B显示文摘AIM:To investigate the effect of pegylated interferon (IFN) α-2b on specific CD8+ T lymphocytes in patients with chronic hepatitis B (CHB). METHODS:Twenty-one patients with CHB were treated with pegylated IFN α-2b. Periphery blood mononuclear cells were isolated from fresh heparinized blood by Ficoll-Hypaque density gradient centrifugation (density:1.077 g/L,Pharmingen) at weeks 0,4,8,12,and 24,respectively. Frequency of circulating hepatitis B virus (HBV) epitope-specific CD8 T cells was detected by flow cytometry. Cytokines were detected by cytometric bead assay. RESULTS:The frequency of circulating HBV core or env-specific CD8 T cells was higher (P < 0.05),the number of HBV core specific CD8 T cells was greater at week 24 (P < 0.05),the level of Th1-type cytokines [interleukin (IL)-12,tumor necrosis factor-α,and IFN-γ] was higher,while that of Th2-type cytokines (IL-4,IL-6,and IL-10) was lower in responders than in nonresponders (P < 0.05) after pegylated IFN α-2b treatment. The IL-6 level was correlated with HBV DNA (r = 0.597,P = 0.04),while the inducible protein-10 (IP-10) level was correlated with serum alanine aminotransferase (ALT) (r = 0.545,P = 0.005). The IP-10 level at week 8 after pegylated IFN α-2b treatment could predict the normalization of ALT in CHB patients (positive predict value = 56%,negative predict value = 92%). CONCLUSION:Pegylated IFN α-2b can enhance the immune response of CHB patients by increasing the frequency of HBV specific CD8+ T cells and regulating the Th1/Th2 cytokines. | Ji Chen,Yan Wang,Xue-Jie Wu,Jun Li,Feng-Qin Hou,Gui-Qiang Wang,Department of Infectious Diseases,Center for Liver Diseases,Peking University First Hospital,Beijing 100034,China | 2010 | World Journal of Gastroenterology2010，16，48： | 17 |
| 17 | Adjuvant percutaneous radiofrequency ablation of feeding artery of hepatocellular carcinoma before treatment显示文摘AIM:To evaluate the feasibility and efficacy of percutaneous radiofrequency ablation(RFA) of the feeding artery of hepatocellular carcinoma(HCC) in reducing the blood-flow-induced heat-sink effect of RFA.METHODS:A total of 154 HCC patients with 177 pathologically confirmed hypervascular lesions participated in the study and were randomly assigned into two groups.Seventy-one patients with 75 HCCs(average tumor size,4.3 ± 1.1 cm) were included in group A,in which the feeding artery of HCC was identified by color Doppler flow imaging,and were ablated with multiple small overlapping RFA foci [percutaneous ablation of feeding artery(PAA)] before routine RFA treatment of the tumor.Eighty-three patients with 102 HCC(average tumor size,4.1 ± 1.0 cm) were included in group B,in which the tumors were treated routinely with RFA.Contrast-enhanced computed tomography was used as post-RFA imaging,when patients were followed-up for 1,3 and 6 mo.RESULTS:In group A,feeding arteries were blocked in 66(88%) HCC lesions,and the size of arteries decreased in nine(12%).The average number of punctures per HCC was 2.76 ± 1.12 in group A,and 3.36 ± 1.60 in group B(P = 0.01).The tumor necrosis rate at 1 mo post-RFA was 90.67%(68/75 lesions) in group A and 90.20%(92/102 lesions) in group B.HCC recur-rence rate at 6 mo post-RFA was 17.33%(13/75) in group A and 31.37%(32/102) in group B(P = 0.04).CONCLUSION:PAA blocked effectively the feeding artery of HCC.Combination of PAA and RFA significantly decreased post-RFA recurrence and provided an alternative treatment for hypervascular HCC. | Yi-Bin Hou Min-Hua Chen Kun Yan Jin-Yu Wu Wei Yang | 2009 | World Journal of Gastroenterology2009，15，21： | 17 |
| 18 | Qingfei Xiaoyan Wan,a traditional Chinese medicine formula, ameliorates Pseudomonas aeruginosa–induced acute lung inflammation by regulation of PI3K/AKT and Ras/MAPK pathways显示文摘Gram-negative pathogen–induced nosocomial infections and resistance are a most serious menace to global public health. Qingfei Xiaoyan Wan(QF), a traditional Chinese medicine(TCM)formula, has been used clinically in China for the treatment of upper respiratory tract infections, acute or chronic bronchitis and pulmonary infection. In this study, the effects of QF on Pseudomonas aeruginosa–induced acute pneumonia in mice were evaluated. The mechanisms by which four typical antiinflammatory ingredients from QF, arctigenin(ATG), cholic acid(CLA), chlorogenic acid(CGA) and sinapic acid(SPA), regulate anti-inflammatory signaling pathways and related targets were investigated using molecular biology and molecular docking techniques. The results showed that pretreatment with QF significantly inhibits the release of cytokines(TNF-α and IL-6) and chemokines(IL-8 and RANTES),reduces leukocytes recruitment into inflamed tissues and ameliorates pulmonary edema and necrosis. In addition, ATG was identified as the primary anti-inflammatory agent with action on the PI3K/AKT and Ras/MAPK pathways. CLA and CGA enhanced the actions of ATG and exhibited synergistic NF-κB inactivation effects possibly via the Ras/MAPK signaling pathway. Moreover, CLA is speculated to target FGFR and MEK firstly. Overall, QF regulated the PI3K/AKT and Ras/MAPK pathways to inhibit pathogenic bacterial infections effectively. | Yuanyuan Hou Yan Nie Binfeng Cheng Jin Tao Xiaoyao Ma Min Jiang Jie Gao Gang Bai | 2016 | Acta Pharmaceutica Sinica B2016，6，3： | 17 |
| 19 | Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol显示文摘Menopause is associated with dyslipidemia and an increased risk of cardio-cerebrovascular disease.The classic view assumes that the underlying mechanism of dyslipidemia is attributed to an insufficiency of estrogen.In addition to a decrease in estrogen, circulating follicle-stimulating hormone (FSH)levels become elevated at menopause.In this study,we find that blocking FSH reduces serum cholesterol via inhibiting hepatic cholesterol biosynthesis.First,epidemiological results show that the serum FSH levels are positively correlated with the serum total cholesterol levels,even after adjustment by considering the effects of serum estrogen,in addition,the prevalence of hypercholesterolemia is significantly higher in peri-menopausal women than that in premenopausal women.Furthermore,we generated a mouse model of FSH elevation by intraperitoneally injecting exogenous FSH into ovariectomized (OVX)mice,in which a normal level of estrogen (E2)was maintained by exogenous supplementation. Consistently,the results indicate that FSH,independent of estrogen,increases the serum cholesterol level in this mouse model. Moreover,blocking FSH signaling by anti-FSHβ antibody or ablating the FSH receptor (FSHR)gene could effectively prevent hypercholesterolemia induced by FSH injection or high-cholesterol diet feeding.Mechanistically,FSH,via binding to hepatic FSHRs, activates the Gi2α/β-arrestin-2/Akt pathway and subsequently inhibits the binding of FoxO1 with the SREBP-2 promoter,thus preventing FoxO1 from repressing SREBP-2 gene transcription.This effect,in turn,results in the upregulation of SREBP-2,which drives HMGCR nascent transcription and de novo cholesterol biosynthesis,leading to the increase of cholesterol accumulation.This study uncovers that blocking FSH signaling might be a new strategy for treating hypercholesterolemia during menopause, particularly for women in peri-menopause characterized by FSH elevation only. | Yanjing Guo Meng Zhao Tao Bo Shizhan Ma Zhongshang Yuan Wenbin Chen Zhao He Xu Hou Jun Liu Zhenhai Zhang Qiang Zhu Qiangxiu Wang Xiaoyan Lin Zhongli Yang Min Cui Lu Liu Yujie Li Chunxiao Yu Xiaoyi Qi Qian Wang Haiqing Zhang Qingbo Guan Lifang Zhao Shimeng Xuan Huili Yan Yanliang Lin Li Wang Qihang Li Yongfeng Song Ling Gao Jiajun Zhao | 2019 | Cell Research2019，29，2： | 16 |
| 20 | Association of serum uric acid levels with osteoporosis and bone turnover markers in a Chinese population显示文摘最近的证据证明尿酸对一些神经病学的疾病是保护的,但是能在许多新陈代谢、心血管的混乱有害。在这研究,我们在中国男性和绝经后的女性检验了在尿酸铺平的浆液和骨头新陈代谢之间的协会。943 男性和 4256 绝经后的女性的一个总数在上海被招募。浆液的层次尿酸和骨头周转标记(BTM ) 与另外的生物化学的特点一起被检测。另外,胖分发通过 MRI 和图象分析软件被计算,并且骨头矿物质密度(BMD ) 用双精力的 X 光检查 absorptiometry 是坚定的。为绝经后的女性,骨质疏松症的流行与 normouricemic 组(P=4.65E-06 ) 相比在 hyperuricemia 组是显著地更低的。在女性,浆液尿酸水平显著地与骨质疏松症被联系,与机会比率(或) 并且 0.844 的 95% 信心间隔(95% CI )[0.763;0.933 ](P=0.0009 ) 在好久调整以后,身体团索引, HbA1c,瘦集体、内脏、下的胖区域,白朊, 25-hydroxyvitamin D3 [25 (哦) D3 ] ,并且甲状旁腺的荷尔蒙(PTH ) 。在女性,浆液尿酸水平断然与大腿骨的颈的 BMD 被相关( | Dan-dan YAN Jie WANG Xu-hong HOU Yu-qian BAO Zhen-lin ZHANG Cheng HU Wei-ping JIA | 2018 | Acta Pharmacologica Sinica2018，39，4： | 16 |
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