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| 1 | 3D bioactive composite scaffolds for bone tissue engineering显示文摘Bone is the second most commonly transplanted tissue worldwide,with over four million operations using bone grafts or bone substitute materials annually to treat bone defects.However,significant limitations affect current treatment options and clinical demand for bone grafts continues to rise due to conditions such as trauma,cancer,infection and arthritis.Developing bioactive three-dimensional(3D)scaffolds to support bone regeneration has therefore become a key area of focus within bone tissue engineering(BTE).A variety of materials and manufacturing methods including 3D printing have been used to create novel alternatives to traditional bone grafts.However,individual groups of materials including polymers,ceramics and hydrogels have been unable to fully replicate the properties of bone when used alone.Favourable material properties can be combined and bioactivity improved when groups of materials are used together in composite 3D scaffolds.This review will therefore consider the ideal properties of bioactive composite 3D scaffolds and examine recent use of polymers,hydrogels,metals,ceramics and bio-glasses in BTE.Scaffold fabrication methodology,mechanical performance,biocompatibility,bioactivity,and potential clinical translations will be discussed. | Gareth Turnbull Jon Clarke Frederic Picard Philip Riches Luanluan Jia Fengxuan Han Bin Li Wenmiao Shu | 2018 | Bioactive Materials2018，3，3： | 40 |
| 2 | Clinical significance of vascular endothelial growth factor expression and neovascularization in colorectal carcinoma显示文摘AIM: To clarify the association of vascular endothelial growth factor (VEGF) and microvascular density (MVD) expression with the angiogenesis and prognosis of colorectal cancer.METHODS: A total of 97 cases of colorectal cardnomas were examined by immunohistochemical staining (SP method), using anti-VEGF and anti-factor CD34^+ monodonal antibodies,RESULTS: VEGF positive staining was obtained in 68 out of 97 cases (70.1%), and observed mainly in the cytoplasm of tumor cells, and also frequently in stromal cells. VEGF expression was more intense in poorly differentiated adenocarcinoma in comparison with others, but there was no significant correlation between VEGF expression and age,sex and stage. A significant correlation was found between the MVD and grades, and there was no significant relationship between the MVD and age, sex, and stage. The MVD in the VEGF positive group (68 cases) was higher than that in the negative group. Upon multivariate analysis, the significant variables were stage, tumor grade and MVD; VEGF expression was not an independent prognostic factor.CONCLUSION: The expression of VEGF has a significant correlation with MVD; MVD expression has prognostic value but VEGF has not in colon cancer. | Shu Zheng Ming-Yong Han Zuo-Xiang Xiao Jia-Ping Peng Qi Dong Cancer Institute,Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2003 | World Journal of Gastroenterology2003，9，6： | 38 |
| 3 | Maize-soybean strip intercropping: Achieved a balance between high productivity and sustainability显示文摘Intercropping is one of the most vital practice to improve land utilization rate in China that has limited arable land resource. However, the traditional intercropping systems have many disadvantages including illogical field lay-out of crops, low economic value, and labor deficiency, which cannot balance the crop production and agricultural sustainability. In view of this, we developed a novel soybean strip intercropping model using maize as the partner, the regular maize-soybean strip intercropping mainly popularized in northern China and maize-soybean relay-strip intercropping principally extended in southwestern China. Compared to the traditional maize-soybean intercropping systems, the main innovation of field lay-out style in our present intercropping systems is that the distance of two adjacent maize rows are shrunk as a narrow strip, and a strip called wide strip between two adjacent narrow strips is expanded reserving for the growth of two or three rows of soybean plants. The distance between outer rows of maize and soybean strips are expanded enough for light use efficiency improvement and tractors working in the soybean strips. Importantly, optimal cultivar screening and increase of plant density achieved a high yield of both the two crops in the intercropping systems and increased land equivalent ratio as high as 2.2. Annually alternative rotation of the adjacent maize-and soybean-strips increased the grain yield of next seasonal maize, improved the absorption of nitrogen, phosphorus, and potasium of maize, while prevented the continuous cropping obstacles. Extra soybean production was obtained without affecting maize yield in our strip intercropping systems, which balanced the high crop production and agricultural sustainability. | DU Jun-bo HAN Tian-fu GAI Jun-yi YONG Tai-wen SUN Xin WANG Xiao-chun YANG Feng LIU Jiang SHU Kai LIU Wei-guo YANG Wen-yu | 2018 | Journal of Integrative Agriculture2018，17，4： | 34 |
| 4 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 5 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 6 | Left posterior fascicular block: a new endpoint of ablation for verapamil-sensitive idiopathic ventricular tachycardia显示文摘Background Verapamil-sensitive, idiopathic left ventricular tachycardia (ILVT) with right bundle branch block configuration and left-axis deviation is known to be due to re-entry mechanism but the exact nature of reentrant circuit in ILVT is not fully elucidated. Radiofrequency (RF) ablation was applied during ventricular tachycardia (VT) and termination of the VT or abolishing the inducibility of the tachycardia was used as an endpoint for successful RF. In this study, the left posterior fascicular block in surface electrocardiogram (ECG) was used as a new endpoint of ablation to cure ILVT. Methods Electrophysiological studies and radiofrequency ablation were performed in 39 consecutive patients [30 men, 9 women; age ranging from 10 to 64 years, mean (29±16) years] with verapamil-sensitive ILVT and structurally normal hearts. VT could be terminated by the intravenous administration of verapamil in all patients. The target site was the midseptum of LV where the earliest Purkinje potentials were recorded during VT. RF current was applied to the target site with or without late diastolic potential (LDP) during sinus rhythm in 37 patients and during VT in 2 patients to meet the ablation endpoint: the left posterior fascicular block in the surface ECG. Results Thirty-seven patients with ILVT had been treated by RF ablation during sinus rhythm and two had been treated during VT. All of them met the endpoint of the left posterior fascicular block. Thirty-eight cases were symptom-free without medications during the follow-up period (range from 3 to 95 months, median 17 months). One patient developed a clinical recurrence and the left posterior fascicular block in surface ECG disappeared. The patient received another treatment. The endpoint was met and the procedure was successful. Conclusions The left posterior fascicular block in surface ECG used as an endpoint of RF ablation to treat ILVT is effective. It is important especially in those patients whose VT can not be induced or the inducible condition is unstable. The effective endpoint implied that the left posterior fascicle might be a critical part of the re-entrant circuit. | MA Fu-sheng MA Jian TANG Kai HAN Hao JIAYu-he FANG Pi-hua CHU Jian-min PU Jie-lin ZHANG Shu | 2006 | Chinese Medical Journal2006，，5： | 21 |
| 7 | Berberine Ameliorates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Rats via Activation of SIRT3/AMPK/ACC Pathway显示文摘This study aimed to verify the effects of berberine(BBR)on the fat metabolism proteins involved in the sirtuin 3(SIRT3)/adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)pathway in the liver tissues of rats with high-fat diet(HFD)-induced non-alcoholic fatty liver disease(NAFLD).Forty-eight rats were randomly divided into the normal control(NC)group,HFD group or BBR group,with 16 rats in each group.After 8 and 16 weeks of treatment,serum and liver samples were collected.Subsequently,body parameters,biochemical parameters and liver pathology were examined.The expression levels of proteins involved in the SIRT3/AMPK/ACC pathway in the liver were detected by Western blotting.After 8 and 16 weeks of a HFD,the successful establishment of rat models with different degrees of NAFLD was confirmed by hematoxylin and eosin(H&E)and Oil Red O staining.NAFLD rat models exhibited obesity and hyperlipidemia,and the protein expression levels of SIRT3,p-AMPK.p-ACC,and CPT-1A in the liver were significantly decreased compared to those in the NC group.The concurrent administration of BBR with the HFD effectively improved serum and liver lipid profiles and ameliorated liver injury.Furthermore,the protein expression levels of SIRT3,p-AMPK,p-ACC,and CPT-1 A in the liver were significantly increased in the BBR group as compared with those in the HFD group.In conclusion,our data suggest that the mechanism by which BBR ameliorates HFD-induced hepatic steatosis may be related to the activation of the SIRT3/AMPK/ACC pathway in the liver. | Yu-pei ZHANG Yuan-jun DENG Kai-rui TANG Run-sen CHEN Shu LIANG Yin-ji LIANG Li HAN Ling JIN Zi-en LIANG Yan-ning CHEN Qin-he YANG | 2019 | Current Medical Science2019，39，1： | 21 |
| 8 | A New Platy-armored Worm from the Early Cambrian Chengjiang Lagerstatte,South China显示文摘The Lower Cambrian Chengjiang Lagerstatte provides a good window to explore the origin and radiation of early bilaterians. Here we describe a netted sclerite-bearing worm Tabelliscolex hexagonus gen. et sp. nov., and tentatively assign it to palaeoscolecidans of priapulids. The cuticle of the animal is covered with two kinds of platy sclerites which are constructed by hexagonally arranged tubercles. Similar structures of the sclerites can be seen on some Cambrian palaeoscolecidans and lobopods, so, this new species is critical for understanding the relationships between lobopods and palaeoscolecidans. | HAN Jian, ZHANG Xingliang, ZHANG Zhifei and SHU Degan Early Life Institute and Department of Geology, Northwest University, Xi’an, Shaanxi 710069, | 2003 | Acta Geologica Sinica(English Edition)2003，77，1： | 15 |
| 9 | Relationship of Inflammation and Endothelial Dysfunction with Risks to Cardiovascular Disease among People in Inner Mongolia of China显示文摘Objective To explore the relationship of inflammation and endothelial dysfunction with risks to cardiovascular disease(CVD).Methods Blood pressure,body weight,body height,waist circumference and lifestyle risk factors were measured and studied among 2589 participants in Inner Mongolia of China,and biomarkers of inflammation and endothelial dysfunction including high-sensitivity C-reactive protein(hsCRP),soluble inter-cellular adhesion molecule-1(sICAM-1),soluble E-selectin(sE-selectin),and angiotensin II were investigated.Results Subjects with metabolic risk factors for CVD had higher levels of hsCRP,sE-selectin and sICAM-1 than those without such risk factors(all P<0.05).Levels of all biomarkers positively and significantly increased with aggregation of the metabolic risk factors among the subjects(all P for trend<0.001).Data from the multivariate analysis showed that participants with high levels of hsCRP [odds ratio(OR): 1.96,95% confidence interval(CI): 1.52-2.53],sE-selectin(OR: 1.35,95% CI: 1.05-1.72),and angiotensin II(OR: 1.81,95% CI: 1.40-2.33) were more likely to develop hypertension; participants with high levels of hsCRP(OR: 2.33,95% CI: 1.85-2.94),sE-selectin(OR: 1.24,95% CI: 1.00-1.54),and sICAM-1(OR: 1.70,95% CI: 1.30-2.22) were more likely to develop dyslipidemia,and those with high levels of hsCRP(OR: 2.95,95% CI: 2.27-3.83) and sICAM-1(OR: 2.80,95% CI: 2.06-3.80) were more likely to develop hyperglycemia.Conclusion Biomarkers of inflammation and endothelial dysfunction were separately associated with relevant metabolic risk factors for CVD.And appropriate measures should be taken to control inflammation and improve endothelial function among individuals with different metabolic risk factors for CVD. | PENG Hao HAN Shu Hai LIU Hai Ying Vasisht CHANDNI CAI Xiao Qing ZHANG Yong Hong | 2013 | Biomedical and Environmental Sciences2013，26，10： | 15 |
| 10 | A new triterpene glycoside from sea cucumber Holothuria leucospilota显示文摘A new triterpene glycoside, leucospilotaside A, along with a known saponin, isolated from sea cucumber Holothuria leucospilota, and its structure was elucidated as 3β-O-[4-O-sodiumsulfate-β-D-quinovopyranosyl-(1→2)-β-D-xylopyranosyl]-holosta-22-ketone-9-en-17α,25α-diol (1) by extensive spectroscopic analysis and chemical methods. Leucospilotaside A (1) has a ketone carbonyl group (22) in the aglycon side chain. | Hua Han Yang Hua Yi Ling Li Xiao Hua Wang Bao Shu Liu Peng Sun Min Xiang Pan | 2007 | Chinese Chemical Letters2007，18，2： | 13 |
| 11 | The earliest-known ancestors of Recent Priapulomorpha from the Early Cambrian Chengjiang Lagerstatte显示文摘The taxonomy of an early ancestor of Recent Priapulidae, Xiaoheiqingella peculiaris (= Yunnanpriapulus halteroformis Huang et al., 2004) from the Early Cambrian Chengjiang fossil Lagerst?tte, is revised. Morphological characters comprise a pair of caudal appendages rather than a single appendage flanking the trunk end and a possible urogenital duct found inside the preanal region. An addi- tional extremely rare fossil priapulid worm, Paratubiluchus bicaudatus gen. nov., sp. nov. is also described herein. Its diagnostic characters are: an introvert bearing 25 longitudi- nal rows of scalids, a distinct neck region, no annulus on the oval trunk, and a pair of caudal appendages. The proportion of body parts is similar in size to that of loricate larvae of Recent priapulids and larva-formed Palaeopriapulitidae. Taking account of the features of Xiaoheiqingella, bicaudal appendages are considered to be a synapomorphy of Pri- apulidae and Tubiluchidae. Paratubiluchus gen. nov. is most likely a candidate for the ancestor of the Tubiluchidae; it probably originated from a larva-formed priapulid with 25 rows of scalids, thus representing an intermediate link be- tween the priapulids in mature-form and the priapulids with lorica. | HAN Jian SHU Degan ZHANG Zhifei LIU Jianni | 2004 | Chinese Science Bulletin2004，49，17： | 12 |
| 12 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 13 | SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts显示文摘The ongoing outbreak of Coronavirus Disease 2019(COVID-19)has become a global public health emergency.SARScoronavirus-2(SARS-CoV-2),the causative pathogen of COVID-19,is a positive-sense single-stranded RNA virus belonging to the family Coronaviridae.For RNA viruses,virus-encoded RNA helicases have long been recognized to play pivotal roles during viral life cycles by facilitating the correct folding and replication of viral RNAs.Here,our studies show that SARS-CoV-2-encoded nonstructural protein 13(nsp13)possesses the nucleoside triphosphate hydrolase(NTPase)and RNA helicase activities that can hydrolyze all types of NTPs and unwind RNA helices dependently of the presence of NTP,and further characterize the biochemical characteristics of these two enzymatic activities associated with SARS-CoV-2 nsp13.Moreover,we found that some bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of SARS-CoV-2 nsp13 in a dose-dependent manner.Thus,our findings demonstrate the NTPase and helicase activities of SARS-CoV-2 nsp13,which may play an important role in SARS-CoV-2 replication and serve as a target for antivirals. | Ting Shu Muhan Huang Di Wu Yujie Ren Xueyi Zhang Yang Han Jingfang Mu Ruibing Wang Yang Qiu Ding-Yu Zhang Xi Zhou | 2020 | Virologica Sinica2020，35，3： | 11 |
| 14 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 15 | The analysis of γ-glutamyl transpeptidase gene in different type liver tissues显示文摘AIM: To probe the value of γ-glutamyl transpeptidase (GGT)messenger RNA in monitoring canceration of liver cells andfor early diagnosis of hepatocellular carcinoma (HCC), byresearching the types of GGT messenger RNA (GGTmRNA)in liver tissues and peripheral blood of different hepatopathy.METHODS: The three types of GGTmRNA (A, B, C) in livertissues and peripheral blood from the patients with HCC,noncancerous hepatopathy, hepatic benign tumor, secondarycarcinoma of liver, and healthy persons were detected byreverse-transcription polymerase chain reaction (RT-PCR).RESULTS: (1) In normal liver tissues, type A waspredominantly found (100.00 %), type B was not found,type C was found occasionally (25.00 %). (2) The distributionof types of GGTmRNA in liver tissues with acute hepatitis,chronic hepatitis, cirrhosis, alcoholic hepatopathy was similaras in normal liver tissues (P>0.05), but type B was found in3 of 18 patients with chronic hepatitis (16.67 %), and alsoin 3 of 11 patients with cirrhosis (27.27 %). (3) There wasno significant difference of types of GGTmRNA between livertissues with hepatic benign tumor, secondary carcinoma ofliver and normal liver tissues (P>0.05). (4) Type B waspredominant in cancerous tissues with HCC (87.5 %), theprevalence of type B in cancerous tissues was significantlyhigher than that in normal liver tissues (0/12) (P<0.05), butthe prevalence of type A in cancerous tissues (46.88 %)was significantly lower than that in normal liver tissues(100.00 %) (P<0.05), and the prevalence of type C (6.25 %)in cancerous was the same as that in normal liver tissues(25.00 %) (P>0.05). In noncancerous tissues of livers withHCC, the main types were type A and type B, the prevalenceof type A (85.71%, 90.48 %) and type C (14.29 %, 9.52 %)in noncancerous tissues of liver with HCC was similar asthat in normal liver tissues (A: 100.00 %; C: 25.00 %)(P>0.05), but the prevalence of type B (80.95 %, 76.19 %)in noncancerous tissues of livers with HCC was significantlyhigher than that in normal liver tissues (0/12) (P<0.05). (5)The prevalence of type B (37.5 %) in peripheral blood withHCC was higher than that in normal person (0/12) (P<0.05).In peripheral blood, type B was found in 4 of 11 cases ofHCC with serum AFP negative.CONCLUSION: The shift of types of GGTmRNA from A toB in liver tissues may be closely related to the developmentof HCC, and the analysis of GGT gene may provide a usefultool for early diagnosis of HCC. | Guo-Qing Han Cheng-Yong Qin Rong-Hua Shu The Center of Liver Diseases, Shandong Provincial Hospital, Clinical Medical College of Shandong University, Jinan 250021, China | 2003 | World Journal of Gastroenterology2003，9，2： | 11 |
| 16 | The ORF3a protein of SARS-CoV-2 induces apoptosis in cells显示文摘Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has caused the ongoing pandemic of Coronavirus Disease 2019.SARS-CoV-2 belongs to the genus Betacoronavirus of the Coronaviridae family,which includes SARS-CoV and Middle East respiratory syndrome coronavirus.1,2 Coronavirus-encoded accessory proteins play critical roles in virus–host interactions and the modulation of host immune responses,thereby contributing to coronaviral pathogenicity via different strategies.3 However,the functions of SARS-CoV-2-encoded accessory proteins are not well understood.Apoptosis is a predominant type of programmed cell death,and has been recognized as an important host antiviral defense mechanism that controls viral infection and regulates the inflammatory response.4,5 Previous studies have reported that the SARS-CoV-encoded accessory protein ORF3a can induce apoptosis in cells,6,7 leading to the question of whether SARS-CoV-2 ORF3a also has pro-apoptotic activity.Here,we investigated the potential apoptosis-inducing activity of SARS-CoV-2 ORF3a in different cell lines and compared the pro-apoptotic activities of SARS-CoV-2 ORF3a with those of SARS-CoV ORF3a using the same system. | Yujie Ren Ting Shu Di Wu Jingfang Mu Chong Wang Muhan Huang Yang Han Xue-Yi Zhang Wei Zhou Yang Qiu Xi Zhou | 2020 | Cellular & Molecular Immunology2020，17，8： | 11 |
| 17 | Identification and Typing of Respiratory Adenoviruses in Guangzhou, Southern China Using a Rapid and Simple Method显示文摘Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China. | Guiyuan Han Hongling Niu Suhui Zhao Bing Zhu Changbing Wang Yungang Liu Mingjie Zhang Shu Yang Feitong Liu Chengsong Wan Qiwei Zhang | 2013 | Virologica Sinica2013，28，2： | 10 |
| 18 | Development of synthetic volatile attractant for male Ectropis obliqua moths显示文摘The tea geometrid Ectropis obliqua is one of the most serious leaf-feeding insect pests in tea(Camellia sinensis) in East Asia. Although several volatile chemicals emitted from tea plants have been reported to be attractive to E. obliqua moths, no synthetic attractants for E. obliqua moths have been developed. By measuring the behavioral responses of the moth to a series of chemicals in the lab, we found that a blend containing a ternary mixture containing(Z)-3-hexenal,(Z)-3-hexenyl hexanoate and benzyl alcohol clearly attracted to E. obliqua moths of both sex and that(Z)-3-hexenyl acetate could enhance the attractiveness of the ternary blend. Moreover, we found that the volatiles emitted from the plant-E. obliqua larva complex have the same attractiveness as: 1) the blend of volatiles containing the ternary mixture and 2) the blend containing(Z)-3-hexenyl acetate plus the ternary mixture to both male and female moths. In a field bioassay, more male moths were observed on traps that were baited with the blend containing(Z)-3-hexenyl acetate plus the ternary mixture than on control traps. Our study raises the tantalizing possibility that synthetic blends could be deployed as attractants for pests in the field. | SUN Xiao-ling LI Xi-wang XIN Zhao-jun HAN Juan-juan RAN Wei LEI Shu | 2016 | Journal of Integrative Agriculture2016，15，7： | 10 |
| 19 | Effects of Acute and Chronic Cold Stress on Antioxidant Function in Intestinal Tracts of Chickens显示文摘This study was to investigate the effects of cold stress on the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA) and superoxide dismutase (SOD) in duodenum, jejunum and ileum of chickens. A total of 80 15-day-old male chickens were treated by cold stress with the duration of the acute cold stress being 1, 3, 6, 12, and 24 h, and the chronic cold stress was 5, 10, and 20 days, respectively. Cold stress temperature was (12±1)℃. The chemical colorimetric method was used to detect the changes of the T-AOC, SOD activities and MDA contents. The results showed that compared with the corresponding control group, effects of acute cold stress on the T-AOC in duodenum, jejunum and ileum of chickens significantly (P<0.05) increased firstly and then decreased. Under chronic cold stress, the T-AOC significantly (P<0.05) decreased. Under acute cold stress and chronic cold stress, the MDA contents significantly (P<0.05) increased in duodenum, jejunum and ileum of chickens. The effects of acute cold stress on the SOD activities in duodenum, jejunum and ileum of chickens significantly (P<0.05) increased firstly and then decreased. Under chronic cold stress the SOD activities significantly (P<0.05) decreased in jejunum and ileum, but significantly (P<0.05) decreased firstly and then increased in duodenum. | Xu Ming Yu Xian-yi Li Jin-long Han Yan-hui Li Shu Xu Shi-wen | 2012 | Journal of Northeast Agricultural University(English Edition)2012，19，2： | 10 |
| 20 | A Five-year Surveillance of Carbapenemase-producing Klebsiella pneumoniae in a Pediatric Hospital in China Reveals Increased Predominance of NDM-1显示文摘Objective To characterize carbapenem(CPM)-non-susceptible Klebsiel a pneumoniae(K. pneumoniae) and carbape-nemase produced by these strains isolated from Beijing Children's Hospital based on a five-year surveillance. Methods The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonix100 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis. Results In total, 179 strains of CPM-non-susceptible K. pneumoniae were isolated from January, 2010 to December, 2014. The rates of non-susceptible to imipenem and meropenem were 95.0% and 95.6%, respectively. In the 179 strains, 95(53.1%) strains carried the blaIMP gene, and IMP-4 and IMP-8 were detected in 92(96.8%) and 3(3.2%) IMP-producing isolates, respectively. 65(36.3%) strains carried the blaNDM-1 gene. 6(3.4%) strains carried the blaKPC gene, and KPC-2 were detected in 6 KPC-producing isolates. In addition, New Delhi-Metallo-1(NDM-1) producing isolates increased from 7.1% to 63.0% in five years and IMP-4 producing isolates decreased from 75.0% to 28.3%. Conclusion High frequencies of multiple resistances to antibiotics were observed in the CPM-non-susceptible K. pneumoniae strains isolated from Beijing Children's Hospital. The production of IMP-4 and NDM-1 metallo-β-lactamases appears to be an important mechanism for CPM-nonsusceptible in K. pneumoniae. | DONG Fang LU Jie WANG Yan SHI Jin ZHEN Jing Hui CHU Ping ZHEN Yang HAN Shu Jing GUO Yong Li SONG Wen Qi | 2017 | Biomedical and Environmental Sciences2017，30，8： | 10 |
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