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| 1 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 2 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 3 | Epidemic Update and Risk Assessment of 2019 Novel Coronavirus--China, January 28, 2020显示文摘On December 29,2019,the health authorities in Hubei Province and Wuhan City received a report of a cluster of pneumonia cases of unknown etiology from a local hospital,and all four patients were employees of the Wuhan Huanan Seafood Wholesale Market(HN market for short)(1).By January 8,2020,Chinese authorities had identified a new virus 2019 novel coronavirus(2019-nCoV)linked with the outbreak of disease,which has been named novel coronavirusinfected pneumonia(NCIP)(2).On 15 January,the National Health Commission(NHC)issued guidelines for the diagnosis,treatment,prevention,and control of NCIP.These guidelines directed case monitoring,reporting,diagnosis,treatment,management,close contact management,and laboratory testing. | Wenxiao Tu Houlin Tang Fangfang Chen Yinong Wei Tingling Xu Kaiju Liao Nijian Xiang Guoqing Shi Qun Li Zijian Feng | 2020 | China CDC weekly2020，2，6： | 15 |
| 4 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 5 | Isolated Cases of Plague--Inner Mongolia-Beijing, 2019显示文摘What is already known about this topic?Plague is an acute infectious disease caused by Yersinia pestis(Y.pestis)and is primarily transmitted by rodents.Human can be infected by bites of bacterium-bearing fleas or direct contact with diseased or dead plagueinfected animals.In 2004,the last human plague case was reported in Inner Mongolia Autonomous Region due to skinning a dead hare.What is added by this report?This is the first case of pneumonic plague imported into a major city since the founding of the People's Republic of China.Two primary pneumonic plague cases(Patients A and B)found in residents of Inner Mongolia were confirmed in Beijing on November 12,2019.Another case(Patient C)of Y.pestis was identified as bubonic plague on November 14.Patient A most likely became infected from aerosol exposure to infective droplets while digging on his farm,located in an Meriones unguiculatus(M.unguiculatus)natural plague focus.Patient B became infected from contact with Patient A(her husband).Patient C became infected after skinning a dead hare.There was no epidemiological relationship between the Patient A/B and Patient C.What are the implications for public health practice?When epizootic plague is detected,local health-care providers and the public should be alerted about any possible risks.Public education efforts should focus on promoting personal protection measures. | Yumeng Wang Lei Zhou Mengguang Fan Quanyi Wang Jianyun Li Qun Li Zijian Feng George FGao Cheng Xu Lijuan Chen Wei Li Yanping Zhang | 2019 | China CDC weekly2019，1，1： | 13 |
| 6 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 7 | Soluble CD40 ligand is associated with angiographic severity of coronary artery disease in patients with acute coronary syndrome显示文摘 | Zhao Wei | 2014 | Chinese Medical Journal2014，，12： | 11 |
| 8 | Generation of graphene-based aerogel microspheres for broadband and tunable high-performance microwave absorption by electrospinning-freeze drying process显示文摘 | Fanbin Meng Huagao Wang Wei Zijian Chen Tian Li Chunyuan Li Yu Xuan Zuowan Zhou | 2018 | Nano Research2018，11，5： | 9 |
| 9 | Gold nanoflowers for 3D volumetric molecular imaging of tumors by photoacoustic tomography显示文摘By binding molecular probes that target tumor cells, gold nanoparticles (AuNPs) with superior characteristics have shown great potential in tumor molecular imaging studies. The non-invasive, high-resolution, and three-dimensional imaging of the targeted AuNPs within the tumor is desirable for both diagnosis and therapy. In this study, gold nanoflowers (AuNFs) are presented as a novel contrast agent for photoacoustic tomography (PAT). By binding to folic acid, the molecular probe, the tail-vein injected AuNFs concentrated within the tumor site in mice; this was clearly visualized by three-dimensional (3D) PAT imaging. In addition, toxicity assay proved that AuNFs were harmless to living cells and animals. Our results demonstrate that AuNFs have great potential in tumor molecular imaging. | Yuanyuan Jiang Zijian Deng Dan Yang Xin Deng Qi Li Yinlin Sha Changhui Li Dongsheng Xu | 2015 | Nano Research2015，8，7： | 8 |
| 10 | In vitro thyroid disrupting effects of organic extracts from WWTPs in Beijing显示文摘It is generally known that there are many endocrine disrupting compounds (EDCs) in the effluents from wastewater treatment plants (WWTPs). Most research has focused on the occurrence of estrogenic or androgenic activities, while ignoring that there are environmental chemicals disrupting thyroid system, which is essential for growth and development in both humans and animals. In the present work, a two-hybrid yeast assay was conducted to evaluate the removal efficiencies of agonistic or antagonistic thyroid receptor (TR) mediated effects in different treatment processes of three WWTPs located in Beijing. We found no TR agonistic, but TR antagonistic activities in all processes from the WWTPs. The TR antagonistic activities in organic extracts of water samples were then calibrated regarding to a known TR-inhibitor, amiodarone hydrochloride (AH). The observed concentration of TR disrupting substances ranged from 2.35 × 10 8 to 6.19 × 10 7 mol/L AH in Gaobeidian WWTP, 3.76 × 10 8 to 8.75 × 10 8 mol/L AH in Lugouqiao WWTP, and 4.80 × 10 9 to 2.55 × 10 8 mol/L AH in Beixiaohe WWTP. Of the three WWTPs, the removal rates were 92.7%, 42.2%, and 23.1% respectively. Industrial sewage may contain more TR disrupting substances compared with domestic sewage. The recipient waters were found to contain considerable concentrations of TR disrupting substances that may cause adverse effects on the exposed organisms. | Na Li Mei Ma Kaifeng Rao Zijian Wang | 2011 | Journal of Environmental Sciences2011，23，4： | 7 |
| 11 | Internalization of the TGF-β type I receptor into caveolin-1 and EEA1 double-positive early endosomes显示文摘 | Kangmin He Xiaohua Yan Nan Li Song Dang Li Xu Bing Zhao Zijian Li Zhizhen Lv Xiaohong Fang Youyi Zhang Ye-Guang Chen | 2015 | Cell Research2015，25，6： | 7 |
| 12 | Determination of estrogens and estrogenic activities in water from three rivers in Tianjin, China显示文摘Studies on estrogenic disrupting compounds(EDCs) occurrence and identification of main responsible compounds in river water discharged into the sea are of significance.In the present research,we screened estrogenic activities of 10 river water samples from 3 main rivers discharged into Bohai Sea in Tianjin using a recombinant two-hybrid yeast assay and chemical analysis by gas chromatography-mass spectrometry.All sample extracts induced significant estrogenic activity,with 17β-estradiol equivalents(EEQ) of raw water ranging from 5.72 to 59.06 ng/L.Six most concerned EDCs in the river water samples including estrone,17β-estradiol,17α-ethinylestradiol,estriol,diethylstilbestrol and estradiol valerate were determined,with their concentrations up to 50.70,31.40,24.40,37.20,2.56,and 8.47 ng/L,respectively.Through causality analysis by comparing the EEQ values of yeast assay and chemical analysis,17α-ethinylestradiol and 17β-estradiol were identified as the main contributors to the estrogenic effects of the river samples,accounting for the whole estrogenic activities(62.99% to 185.66%),and estrogen antagonistic compounds might presented in the heavy polluted water samples.The proposed approach using both chemical analysis and bioassay could be used for identification and evaluation of the estrogenic activity of EDCs in river water. | Kaifeng Rao Bingli Lei Na Li Mei Ma Zijian Wang | 2013 | Journal of Environmental Sciences2013，25，6： | 6 |
| 13 | Highly effective and reproducible surface-enhanced Raman scattering substrates based on Ag pyramidal arrays显示文摘 | Yandong Wang Nan Lu Wentao Wang Lingxiao Liu Lei Feng Zhoufang Zeng Haibo Li Weiqing Xu Zijian Wu Wei Hu Yanqing Lu Lifeng Chi | 2013 | Nano Research2013，6，3： | 6 |
| 14 | Application of probability integral method in ground deformation prediction显示文摘In order to study the law of mining subsidence and ground movement, to provide the basis of coal mining under building, railway and water, we used the probability integration method to make comprehensive evaluation of ground stability. Take Yingcheng Coal Mine of Jiutai as an example. Mining-induced movement and horizontal movement are analyzed on the basis of the measurement data. The results of prediction can provide reference and basis for prevention of coal mining subsidence and future restoration and treatment. | WANG Zijian LI Guangjie YOU Bing BAO Shuochao | 2012 | Global Geology2012，15，3： | 5 |
| 15 | Soil microbial activity and nutrients of evergreen broad-leaf forests in mid-subtropical region of China显示文摘To better understand the effects of forest succession on soil microbial activity, a comparison of soil microbial properties and nutrients was conducted between three forest types representing a natural forest succession chronosequence. The study compared a pine(Pinus massoniana) forest(P_F), a pine and broadleaf mixed forest(M_F) and an evergreen broadleaf forest(B_F), in the Yingzuijie Biosphere Reserve, Hunan Province, China.Results showed that soil nutrients in the M_Fand BFplots were higher than in the P_Fplots. The range in microbial biomass carbon followed a similar pattern with B_Fhaving the greatest values, 522–1022 mg kg^-1, followed by M_F368–569 mg kg^-1, and finally, P_F193–449 mg kg^-1. Soil nutrients were more strongly correlated with microbial biomass carbon than basal respiration or metabolic quotient. Overall, forest succession in the study site improved soil microbial properties and soil fertility, which in turn can increase primary productivity and carbon sequestration. | Zhangquan Zeng Silong Wang Canming Zhang Hong Tang Xiquan Li Zijian Wu Jia Luo | 2015 | Journal of Forestry Research2015，26，3： | 5 |
| 16 | Maternal exposure to triclosan constitutes a yet unrecognized risk factor for autism spectrum disorders显示文摘Dear Editor,Autism spectrum disorders(ASD)are complex neurodevelopmental disorders characteristic of core behavioral traits like restricted,repetitive behaviors and deficiency in social interactions.1 The prevalence of ASD has rapidly increased worldwide,rising from 1/149 in 2000,1/68 in 2012,and to?1/40 in 2016 of children at the age of 3-17 in the United States.2 ASD prevalence was found to be associated with socioeconomic status(SES)as overpresented in high-income households,3 and to be significantly enriched in certain regions,e.g.,reaching up to 4.88%in Florida.2 In the last decades,advances in genetic studies have led to unprecedented understanding of the genetic factors that were associated with<50%of human ASD. | Zijian Hao Qionghui Wu Zhengwei Li Yali Li Qiu Li Xi Lai Huan Liu Menghuan Zhang Ting Yang Jie Chen Yaping Tang Jingkun Miao Huatai Xu Tingyu Li Ronggui Hu | 2019 | Cell Research2019，29，10： | 5 |
| 17 | Retinoid X receptor activities of source waters in China and their removal efficiencies during drinking water treatment processes显示文摘There is increasing evidence of estrogenic activities of source waters and drinking waters in China based on estrogen receptors(ERs) testing.However,relating such activities to retinoid X receptors(RXRs) in both drinking and source waters are lacking.To rectify this situation,we assessed 23 source water samples from six major river systems in China.We also collected samples at various stages of water processing from three drinking water treatment plants(DWTPs) using a two-hybrid RXR yeast assay with and without metabolism.No RXR agonistic activity was observed,but significant antagonistic activity was detected in all sample extracts.The RXR antagonistic activities of source water sample extracts ranged from 15.2% to 57.8% without metabolism and 11.5% to 68.3% with metabolism,respectively.In the drinking water treatment processes,RXR antagonistic activities without metabolism and with metabolism of up to 31.4% and 37.5% were removed,respectively.Nevertheless,the remaining RXR antagonists in treated drinking water from these source waters could still be harmful to human health.To the best of our knowledge,the occurrence of in vitro RXR disruption activities in source and drinking water has not been previously reported in China.Therefore,an attempt was made to conduct detailed studies investigating RXR disrupting activities and their possible risks in source and drinking water. | JIANG WeiWei YAN Ye LI Na MA Mei WANG DongHong RAO KaiFeng WANG ZiJian SATYANARAYANAN Senthi Kumaran | 2012 | Chinese Science Bulletin2012，57，6： | 5 |
| 18 | Proteasomal deubiquitinase UCH37 inhibits degradation of β-catenin and promotes cell proliferation and motility显示文摘The ubiquitin–proteasome system degrades most cellular proteins in eukaryotes.UCH37,also known as UCH-L5,is a deubiquitinase binding to Rpn13,a receptor for ubiquitinated substrates in the 26 S proteasome.But,it remains unclear how UCH37 influences the proteasomal degradation of the ubiquitinated substrates.Because deletion of UCH37 is embryonically lethal in mice,this study aims to investigate the role of UCH37 in proteasomal degradation by constructing the UCH37-deficient cell lines using CRISPR/Cas9 technology.Our results demonstrated that deletion of UCH37 decreased the levels of proteasomal Rpn13,implying that UCH37 might facilitate incorporation of Rpn13 into the proteasome.Meanwhile,deletion of UCH37 decreased the levels of β-catenin and the early endosomal protein Rab8.β-Catenin interacts with TCF/LEF to control transcription,and is involved in development,tissue homeostasis and tumorigenesis.We further found that deletion of UCH37 increased the levels of the ubiquitinated β-catenin and accelerated the hydrogen peroxide-stimulated degradation of β-catenin.Deletion of UCH37 also down-regulated the transcription of c-Myc,a downstream effector of β-catenin,and inhibited cell proliferation and motility.These results raise the possibility that UCH37 maintains the homeostasis of proteasomal degradation reciprocally by assisting the recruitment of the ubiquitin receptor Rpn13 into the proteasome and by reversing ubiquitination of certain critical substrates of the 26 S proteasome. | Zijian Li Luming Zhou Tianxia Jiang Libin Fan Xiaoying Liu Xiaobo Qiu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 4 |
| 19 | Study on the Water Quality and Protection Measures of Dongting Lake显示文摘From nitrogen and phosphorus,chemical oxygen demand,phytoplankton,and eutrophication,this article analyzes the current situation of water quality in the Dongting Lake area,and discusses the factors influencing the water quality of Dongting Lake. Based on the actual production,geographic characteristics and outstanding problem of grim water pollution situation in the Dongting Lake area,some specific proposed measures are put forward in order to provide the basis and reference for the future pollution control in the Dongting Lake area,such as strengthening the industrial pollution control,developing ecological agriculture,and enhancing ecological restoration and water quality early warning. | Zhangquan ZENG Canming ZHANG Xiquan LI Nan Yang Yandong NIU Zijian WU | 2015 | Asian Agricultural Research2015，7，1： | 4 |
| 20 | Sustained‑Release Nanocapsules Enable Long‑Lasting Stabilization of Li Anode for Practical Li‑Metal Batteries显示文摘A robust solid-electrolyte interphase(SEI)enabled by electrolyte additive is a promising approach to stabilize Li anode and improve Li cycling efficiency.However,the self-sacrificial nature of SEI forming additives limits their capability to stabilize Li anode for long-term cycling.Herein,we demonstrate nanocapsules made from metal–organic frameworks for sustained release of LiNO3 as surface passivation additive in commercial carbonate-based electrolyte.The nanocapsules can offer over 10 times more LiNO3 than the solubility of LiNO3.Continuous supply of LiNO3 by nanocapsules forms a nitride-rich SEI layer on Li anode and persistently remedies SEI during prolonged cycling.As a result,lifespan of thin Li anode in 50μm,which experiences drastic volume change and repeated SEI formation during cycling,has been notably improved.By pairing with an industry-level thick LiCoO2 cathode,practical Li-metal full cell demonstrates a remarkable capacity retention of 90%after 240 cycles,in contrast to fast capacity drop after 60 cycles in LiNO3 saturated electrolyte. | Qianqian Liu Yifei Xu Jianghao Wang Bo Zhao Zijian Li Hao Bin Wu | 2020 | Nano-Micro Letters2020，12，12： | 4 |
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