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| 1 | Microstructural characteristics and mechanical behavior of B4Cp/6061Al composites synthesized at different hot-pressing temperatures显示文摘B4Cp/6061Al composites have become important structural and functional materials and can be fabricated by powder metallurgy and subsequent hot rolling. In this work, the effects of the hot-pressing temperature on microstructures and mechanical behaviors of the B4Cp/6061Al composites were investigated. The results showed that compared with the T4 heat treated B4Cp/6061Al composite hot pressed at 560℃, the yield strength and failure strain of the composites hot pressed at 580℃ were increased to 235 MPa and 18.4%, respectively. This was associated with the interface bonding strength between the B4C particles and the matrix. However, the reaction products, identified to be MgAl2O4 phases, were detected in the composites hot pressed at 600℃. The formation of the MgAl2O4 phases resulted in the Mg depletion, thus reducing the yield strength to 203.5 MPa after the T4 heat treatment due to the effect of the solid solution strengthening being weakened. In addition, the variation of hardness and electrical conductivity was mainly related to the Mg content in the matrix. Based on the as-rolled microstructures observed by SEM, SR-μCT and fracture surfaces, the deformation schematic diagram was depicted to reflect the tensile deformation process of the composites. | Minqiang Gao Zongning Chen Huijun Kang Enyu Guo Rengeng Li Ying Fu Honglan Xie Tongmin Wang | 2019 | Journal of Materials Science & Technology2019，35，8： | 4 |
| 2 | Combined detection and subclass characteristics analysis of CTCs and CTECs by SE-iFISH in ovarian cancer显示文摘Objective:Hematogenous metastasis is essential for the progression of ovarian cancer(OC),and circulating tumor cells(CTCs)are part of the metastatic cascade.However,the detection rate of CTC is low due to the use of less sensitive detection methods.Therefore,this study aimed to detect CTCs and circulating tumorigenic endothelial cells(CTECs)in patients with OC using subtraction enrichment and immunostaining and fluorescence in situ hybridization(SE-iFISH).Methods:We enrolled a total of 56 subjects,including 20 OC patients and 36 ovarian benign tumor patients.CTCs and CTECs were captured by subtraction enrichment(SE)and counted and classified according to immunofluorescence staining of tumor markers(TMs)carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)combined with fluorescence in situ hybridization(iFISH)of chromosome 8(Chr8)aneuploidy.The diagnostic value and subtype characteristics of CTCs and CTECs were investigated.Results:The detection rate of CTCs by SE-iFISH was high.Compared with CA125 and HE4,Chr8 aneuploidy was the major identification feature of CTC.CTC counts in OC were statistically higher than those in benign groups.CTC and CTEC with≥pentaploidy were detected in both groups,illustrating the poor diagnostic value of CTC or CTEC.Distributions of triploid and tetraploid CTC subtypes were significantly different,and combined detection of triploid and tetraploid CTCs showed the best diagnostic value.In contrast,the distribution of CTECs in the OC and benign groups had no statistically significant difference.Small CTCs accounted for over 1/3 of the total CTC count.We also found that small CTCs and CTECs primarily comprised triploid cells,while large CTCs and CTECs mainly comprised pentaploidy and beyond.Conclusions:The application of SE-iFISH offered a more comprehensive understanding of heterogeneous CTCs and CTECs in OC.Analysis of subclass characteristics of the CTCs and CTECs according to Chr8 aneuploidy and cell size may broaden their potential clinical utility and deepen mechanistic studies in OC. | Hongyan Cheng Shang Wang Wenqing Luan Xue Ye Sha Dou Zhijian Tang Honglan Zhu Peter Ping Lin Yi Li Heng Cui Xiaohong Chang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 3 |
| 3 | Gather wisdom to overcome barriers:Well-designed nano-drug delivery systems for treating gliomas显示文摘Due to the special physiological and pathological characteristics of gliomas,most therapeutic drugs are prevented from entering the brain.To improve the poor prognosis of existing therapies,researchers have been continuously developing non-invasive methods to overcome barriers to gliomas therapy.Although these strategies can be used clinically to overcome the blood-brain barrier(BBB),the accurate delivery of drugs to the glioma lesions cannot be ensured.Nano-drug delivery systems(NDDS)have been widely used for precise drug delivery.In recent years,researchers have gathered their wisdom to overcome barriers,so many well-designed NDDS have performed prominently in preclinical studies.These meticulous designs mainly include cascade passing through BBB and targeting to glioma lesions,drug release in response to the glioma microenvironment,biomimetic delivery systems based on endogenous cells/extracellular vesicles/protein,and carriers created according to the active ingredients of traditional Chinese medicines.We reviewed these well-designed NDDS in detail.Furthermore,we discussed the current ongoing and completed clinical trials of NDDS for gliomas therapy,and analyzed the challenges and trends faced by clinical translation of these well-designed NDDS. | Jiwei Cui Yuanxin Xu Haiyan Tu Huacong Zhao Honglan Wang Liuqing Di Ruoning Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，3： | 2 |
| 4 | Effect of p62 on tau hyperphosphorylation in a rat model of Alzheimer's disease显示文摘Tau hyperphosphorylation is a main cause of neuronal loss in Alzheimer's disease,which can be caused by many factors,including oxidative stress.The multifunctional protein p62,which exists in neurofibrillary tangles and causes aggregation of hyperphosphorylated tau,not only serves as a receptor in selective autophagy,but also regulates oxidative stress.However,whether p62 partici-pates in oxidative stress-induced tau hyperphosphorylation remains unclear.In this study,we pro-duced an Alzheimer's disease rat model by injecting β-amyloid protein into the hippocampus and β-galactose intraperitoneally.Hematoxylin-eosin staining was used for morphological analysis of brain tissue,and western blotting,immunohistochemistry and reverse transcription-PCR were em-ployed to study p62 and autophagy related proteins,antioxidant defense system kelch-like ECH-associated protein 1-NF-E2-related factor 2 related proteins and hyperphosphorylated tau,respectively.The number of neurons in the brain decreased in Alzheimer's disease rats,and the autophagy related proteins Atg12-Atg5,microtubule-associated protein 1 light chain 3-phosphatidylethanolamine and Beclin1 increased significantly,while p62 expression reduced.Expression of kelch-like ECH-associated protein 1 increased,NF-E2-related factor 2 protein and the downstream gene products of glutamate cysteine ligase catalytic subunit and glutamate cysteine ligase modulatory subunit decreased,and hyperphosphorylated tau increased.These findings demonstrate that autophagy levels increased and p62 levels decreased in the brains of Alzheimer's disease rats.Moreover,the anti-oxidative capability of the NF-E2-related factor 2-antioxidant re-sponse element pathway was decreased,which may be the cause of tau hyperphosphorylation in Alzheimer's disease brain tissue and the subsequent structural and functional damage to neurons. | Xianhong Zheng Weiwei Wang Ruizhi Liu Honglan Huang Rihui Zhang Liankun Sun | 2012 | Neural Regeneration Research2012，7，17： | 2 |
| 5 | Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis显示文摘Hemophagocytic lymphohistiocytosis(HLH)is a highly fatal condition with the positive feedback loop between continued immune cell activation and cytokine storm as the core mechanism to mediate multiple organ dysfunction.Inspired by macrophage membranes harbor the receptors with special high affinity for proin-flammation cytokines,lipopolysaccharide(LPS)-stimulated macrophage membrane-coated nanoparticles(LMNP)were developed to show strong sponge ability to both IFN-γand IL-6 and suppressed overactivation of macrophages by inhibiting JAK/STAT signaling pathway both in vitro and in vivo.Besides,LMNP also efficiently alleviated HLH-related symptoms including cytopenia,hepatosplenomegaly and hepatorenal dysfunction and save the life of mouse models.Furthermore,its sponge effect also worked well for five human HLH samples in vitro.Altogether,it’s firstly demonstrated that biocompatible LMNP could dampen HLH with high potential for clinical transformation,which also provided alternative insights for the treatment of other cytokine storm-mediated pathologic conditions such as COVID-19 infection and cytokine releasing syndrome during CAR-T therapy. | Honglan Wang Huiwen Liu Jia Li Chunying Liu Hui Chen Junying Li Chunyan Sun Tao Guo Zhiqing Pang Bo Zhang Yu Hu | 2023 | Bioactive Materials2023，，3： | 1 |
| 6 | Dietary fat intake and liver cancer risk: A prospective cohort study in Chinese women显示文摘Objective:This study aimed to determine whether dietary fat intake increased liver cancer risk in Chinese women from a prospective population-based cohort.Methods:A total of 72,704 Chinese women were followed up from the time of baseline recruitment(1996–2000)to the end of 2016.Dietary fat intake was calculated using a validated food frequency questionnaire.The Cox regression model was used to assess the hazard ratio(HR)and 95%confidence intervals(CI)for dietary fat intake and liver cancer risk.Results:We identified 252 incident liver cancer cases out of 1,267,845 person-years during the overall follow-up time.Null associations,neither in quartiles nor per standard deviation(SD)increment,were detected between liver cancer risk and dietary total fat,fat subtypes and subtype ratios,and food sources.The HR(95%CI)of the 1-SD increment was 1.03(0.90–1.17)for total fat,1.06(0.93–1.20)for saturated fat,1.06(0.93–1.21)for monounsaturated fat,and 1.00(0.89–1.13)for polyunsaturated fat.Similar null associations were observed in stratification analyses according to body mass index and menopausal status.Conclusions:In our prospective cohort study,no significant association was observed in Chinese women between dietary fat and liver cancer risk,and in stratification and sensitivity analyses. | Xiaowei Ji Jing Wang Zhuoying Li Qiuming Shen Jiayi Tuo Jinghao Bi Yuting Tan Honglan Li Yongbing Xiang | 2022 | Cancer Biology & Medicine2022，19，3： | 1 |
| 7 | Quality evaluation of Po- lygala japonica through simulataneous determination of six bioactive triterpenoid saponins by HPLC -ELSD 显示文摘 | Wang Honglan Gao Jie Zhu Danni | 2007 | J Pharmaceutical and Bi- omedical Analysis2007，43，4： | 1 |
| 8 | Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD显示文摘 | Honglan Wang Jie Gao Danni Zhu Boyang Yu | 2006 | Journal of Pharmaceutical and Biomedical Analysis2006，，4： | 1 |
| 9 | Hepatitis C virus envelope glycoproteins complementation patterns and the role of the ecto- and transmembrane domains显示文摘 | Xiaojing Lin Yonghui Zhang Shengli Bi Jian Lu Honglan Zhao Wenjie Tan Dexin Li Yue Wang | 2009 | Biochemical and Biophysical Research Communications2009，，2： | 1 |
| 10 | Broad-spectrum and powerful neutralization of bacterial toxins by erythroliposomes with the help of macrophage uptake and degradation显示文摘Anti virulence strategy has been considered as one of the most promising approaches to combat drug-rcesistant bacterial infections.Porc-forming toxins(PFTs)are the largest class of bacterial toxins,inficting their virulence ffect through creating pores on the cell membrane.However,curent solutions for eliminating PFTs are mostly designed based on their molecular structure,requiring customized design for different interactions.In the present study,we employed erythroliposome(denoted as RM-PL),a biomimetic platform constructed by artificial lipid membranes and natural erythrocyle membranes,to necutralize different hemolytic PFTs regardless of their molecular structure.When tested with model PFTs,including a-hemolysin,listeriolysin O,and streptolysin O,RM-PL could completely inhibit toxin-induced hemolysis in a concentration-dependent manner.In vivo studies further confirmed that RM-PL.could eficiently neuralize various toxins and save animals'lives without causing damage to 0rgans or tissues.In addition,we explored the underlying mechanisms of this efficient detoxification ability and found that it was mainly macrophages in the spleen and the liver that took up RM-PL-absorbed toxins through a variety of endocytosis pathways and digested them in lysosomes.In summary,the biomimetic RM-PL presented a promising system for broad-spectrum and powerful toxin neutralization with a mech-anism of lysosome-mediaed loxin degradation. | Chunying Liu Shuangrong Ruan Ying He Xuejing Li Yuefei Zhu Honglan Wang Hanwei Huang Zhiqing Pang | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 1 |
| 11 | Diagnosis and treatment of retroperitoneal bronchogenic cysts: A casereport显示文摘 | Biao Dong Honglan Zhou Jianjian Zhang Yuantao Wang Yaowen Fu | 2014 | Oncology Letters2014，，6： | 1 |
| 12 | Selective DNA detection at Zeptomole level based on coulometric measurement of gold nanoparticle-mediated electron transfer across a self-assembled monolayer显示文摘A selective DNA sensing with zeptomole detection level is developed based on coulometric measurement of gold nanoparticle (AuNPs)-mediated electron transfer (ET) across a self-assembled monolayer on the gold electrode. After immobilization of a thiolated hairpin-structured DNA probe, an alkanethiol monolayer was self-assembled on the resultant electrode to block [Fe(CN)6 ]-3-/4in a solution from accessing the electrode. In the presence of DNA target, hybridization between the DNA probe and the DNA target breaks the stem duplex of DNA probe. Consequently, stem moiety at the 3′-end of the DNA probes was removed from the electrode surface and made available for hybridization with the reporter DNA-AuNPs conjugates (reporter DNA-AuNPs). The thiolated reporter DNA matches the stem moiety at the 3′-end of the DNA probe. AuNPs were then enlarged by immersing the electrode in a growth solution containing HAuCl 4 and H2O2 after the reporter DNA-AuNPs bound onto the electrode surface. The enlarged AuNPs on the electrode restored the ET between the electrode and the [Fe(CN)6]3 -/4- , as a result, amplified signals were achieved for DNA target detection using the coulometric measurement of Fe(CN)6 3- electro-reduction by prolonging the electrolysis time. The quantities of ET on the DNA sensor increased with the increase in DNA target concentration through a linear range of 3.0 fM to 1.0 pM when electrolysis time was set to 300 s, and the detection limit was 1.0 fM. Correspondingly, thousands of DNA (zeptomole) copies were detected in 10L samples. Furthermore, the DNA sensor showed excellent differentiation ability for single-base mismatch. | WANG Wei YUAN XiaQing LIU XuHui GAO Qiang QI HongLan ZHANG ChengXiao | 2013 | Science China Chemistry2013，56，7： | 1 |
| 13 | Transformation from precipitates to vesicles in mixed cationic and anionic surfactant systems显示文摘 | Chuanzhong Wang Shihua Tang Jianbin Huang Xinran Zhang Honglan Fu | 2002 | Colloid and Polymer Science2002，，8： | 1 |
| 14 | Single Particle-Based Confocal Laser Scanning Microscopy for Visual Detection of Copper Ions in Confined Space显示文摘Main observation and conclusion A single particle-based confocal laser scanning microscopy was developed for the visual detection of copper ions in confined space.A fluorescence microparticle,named AuNCs/ZIF-8,was synthesized by coating gold nanoclusters(AuNCs)onto the outer surface of zeolitic imidazolate framework-8(ZIF-8). | Ke Wang Manping Qian Honglan Qi Qiang Gao Chengxiao Zhang | 2021 | Chinese Journal of Chemistry2021，39，7： | 1 |
| 15 | Monitoring casein kinase Ⅱ at subcellular level via bio-bar-code-based electrochemiluminescence biosensing method显示文摘A highly sensitive electrochemiluminescence(ECL) biosensing method was developed for monitoring casein kinase Ⅱ(CK2) at subcellular level via bio-bar-code assay.A bio-bar-code probe(h-DNA/AuNPs/pDNA) prepared by conjugating phosphorylated DNA(p-DNA) and hairpin DNA(h-DNA) onto gold nanoparticles(AuNPs) was used as a carrier for ECL signal reagent(Ru(phen)32+) while a specific peptide was used as a recognition substance.A gold ultramicroelectrode with a diameter of 400 nm was fabricated and then modified with the specific peptide via self-assembly technique to obtain peptide modified gold ultramicroelectrode.The peptide on gold ultramicroelectrode was phosphorylated in the presence of CK2 and adenosine 5’-triphosphate,and then the phosphorylated peptide was integrated with the h-DNA/AuNPs/p-DNA through a process mediated by zirconium cations(Zr4+),and finally Ru(phen)32+ was intercalated into h-DNA.A 'signal on' ECL method was developed for the detection of CK2 in the range of 0.005-0.2 U/mL with a detection limit of 0.001 U/mL.Additionally,combined efficient subcellular phosphorylation in vivo with bio-bar-code-based ECL biosensing method,the ECL method was further applied to monitor CK2 at subcellular level without tedious subcellular fractionation.It was found that the concentration of CK2 by inserting the peptide modified gold ultramicroelectrode into the nucleus was higher than that into cytoplasm of HeLa cells.A distinct heterogeneity among CK2 concentrations in single cells was observed for cellular heterogeneity assessment. | Lifen Wang Jiajia Song Xiaofei Wang Honglan Qi Qiang Gao Chengxiao Zhang | 2020 | Chinese Chemical Letters2020，31，9： | 1 |
| 16 | Enzymatic hydrolysis of rice dreg protein: Effects of enzyme type on the functional properties and antioxidant activities of recovered proteins显示文摘 | Qiang Zhao Hua Xiong Cordelia Selomulya Xiao Dong Chen Honglan Zhong Shenqi Wang Wenjing Sun Qiang Zhou | 2012 | Food Chemistry2012，，3： | 1 |
| 17 | Translating bacteriophage-derived depolymerases into antibacterial therapeutics:Challenges and prospects显示文摘Predatory bacteriophages have evolved a vast array of depolymerases for bacteria capture and deprotection.These depolymerases are enzymes responsible for degrading diverse bacterial surface carbohydrates.They are exploited as antibiofilm agents and antimicrobial adjuvants while rarely inducing bacterial resistance,making them an invaluable asset in the era of antibiotic resistance.Numerous depolymerases have been investigated preclinically,with evidence indicating that depolymerases with appropriate dose regimens can safely and effectively combat different multidrug-resistant pathogens in animal infection models.Additionally,some formulation approaches have been developed for improved stability and activity of depolymerases.However,depolymerase formulation is limited to liquid dosage form and remains in its infancy,posing a significant hurdle to their clinical translation,compounded by challenges in their applicability and manufacturing.Future development must address these obstacles for clinical utility.Here,after unravelling the history,diversity,and therapeutic use of depolymerases,we summarized the preclinical efficacy and existing formulation findings of recombinant depolymerases.Finally,the challenges and perspectives of depolymerases as therapeutics for humans were assessed to provide insights for their further development. | Honglan Wang Yannan Liu Changqing Bai Sharon Shui Yee Leung | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 18 | Highly selective electrochemical method for the detection of serotonin at carbon fiber microelectrode modified with gold nanoflowers and overoxidized polypyrrole显示文摘A highly selective and sensitive electrochemical method was developed for the detection of serotonin (5-hydroxytryptamine, 5-HT) at gold nanoflowers (Au NFs) and overoxidized polypyrrole (OPPy) modified carbon fiber microelectrode (CFME). Carbon fiber was firstly modified with gold nanoflowers using electroless deposition method, and then modified with overoxidized polypyrrole using electrochemical polymerization and overoxidization to obtain OPPy/Au NFs/CFME. The obtained OPPy/Au NFs/CFME was characterized by field emission scanning electron microscopy and electrochemical techniques. It was found that the OPPy/Au NFs/CFME showed good sensitivity for the detection of 5-HT in the range of 10 nmol/L-7.0μmol/L with detection limit of 2.3 nmol/L, and negligible interferences from ascorbic acid, 5-hydroxyindole acetic acid and uric acid. The OPPy/Au NFs/CFME was successfully applied to the detection of 5-HT in human serum samples with good recovery. The work demonstrates that the electrochemical method, incorporating signal amplification of Au NFs with higher cation selection of OPPy, provides a promising tool for the detection of 5-HT in biological systems. | Jiajia Song Lifen Wang Hetong Qi Honglan Qi Chengxiao Zhang | 2019 | Chinese Chemical Letters2019，30，9： | 0 |
| 19 | LPS adsorption and inflammation alleviation by polymyxin B-modified liposomes for atherosclerosis treatment显示文摘Chronic inflammation is critical in the onset and progression of atherosclerosis(AS).The lipopolysaccharide(LPS)level in the circulation system is elevated in AS patients and animal models,which is correlated with the severity of AS.Inspired by the underlying mechanism that LPS could drive the polarization of macrophages toward the M1 phenotype,aggravate inflammation,and ultimately contribute to the exacerbation of AS,LPS in the circulation system was supposed to be the therapeutic target for AS treatment.In the present study,polymyxin(PMB)covalently conjugated to PEGylated liposomes(PLPs)were formulated to adsorb LPS through specific interactions between PMB and LPS.In vitro,the experiments demonstrated that PLPs could adsorb LPS,reduce the polarization of macrophages to M1 phenotype and inhibit the formation of foam cells.In vivo,the study revealed that PLPs treatment reduced the serum levels of LPS and pro-inflammatory cytokines,decreased the proportion of M1-type macrophages in AS plaque,stabilized AS plaque,and downsized the plaque burdens in arteries,which eventually attenuated the progression of AS.Our study highlighted LPS in the circulation system as the therapeutic target for AS and provided an alternative strategy for AS treatment. | Huiwen Liu Honglan Wang Qiyu Li Yiwei Wang Ying He Xuejing Li Chunyan Sun Onder Ergonul Fusun Can Zhiqing Pang Bo Zhang Yu Hu | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 0 |
| 20 | Nanoplateletsomes for rapid hemostasis performance显示文摘Uncontrollable hemorrhage remains staple trouble in surgical procedures and a leading cause after major trauma.The bleeding issue may trigger various pathologic scenarios that can lead to tissue morbidities and mortalities,and currently available on-site hemostatic agents are confined to a narrow therapeutic index and may carry the risk of immunogenicity.Inspired by the crucial role of platelets in the process of thrombus,a platelet-mimetic plateletsome with wound targeting and blood coagulation properties is developed for hemorrhage control.Plateletsome is formulated by integrating platelet membranes with functionalized synthetic liposomes and exhibits superior wound targeting and effective hemostasis properties.It presents less blood loss and shorter hemostasis time than the platelet membrane vehicles or the conventional liposomes in the mouse tail transection model.The strong homing of the biomimetic plateletsome to the thrombus was also confirmed,demonstrating the potential of this engineered cell membrane vesicle as a biomimetic hemostat for bleeding treatment. | Honglan Wang Yuefei Zhu Longlong Zhang Huiwen Liu Chunying Liu Bo Zhang Yanan Song Yu Hu Zhiqing Pang | 2022 | Chinese Chemical Letters2022，33，6： | 0 |
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