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| 1 | 新城疫病毒某水禽分离株经鸡体传代后由非致病型转变为速发型的研究显示文摘最近 ,一株无毒力的新城疫病毒 (NDV)在鸡体内繁殖时 ,变成了强毒株。但至今尚未能证实 ,经鸡体传代的野生水禽新城疫病毒是否也具有变成速发型毒株的能力。为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒 ,我们通过在鸡体内传播鹅源性无毒力株 ,经气囊接种连续传代 9次 ,随后再在鸡脑内传代 5次。结果显示 ,该病毒的毒力变得很强 ,致死率可达 1 0 0 %。通过致病性试验证实 ,其具有典型的速发型病毒特征 ;融合蛋白裂解位点的序列分析表明 ,原始的分离株含有无毒力型毒株共有的裂解序列 :E_R_Q_E_R/L,而通过鸡体反复传代后 ,该序列变为致病性毒株共有的序列 :K_R_Q_K_R/F。这些结果表明 ,野生水禽中自然存在的无毒力毒株 ,具有无毒力株相应的序列 ,但当其在鸡群中传播时 ,则具有变成高致病性病毒的能力。同时研究表明 ,鸡体提供了该病毒从非致病型向致病型转变的选择机制。 | 于圣青 丁铲 NORIKO KISHIDA HIROSHI ITO HIROSHI KIDA KOICHI OTSUKI YOSHIHIRO KAWAOKA TOSHIHIRO ITO | 2003 | 中国预防兽医学报2003，25，1： | 32 |
| 2 | Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts显示文摘有很少研究,调查每天提供的机械刺激的效果低紧张的搏动的超声(LIPUS ) 处理。LIPUS 被知道加速骨头矿化作用和新生;然而,精确细胞的机制是不清楚的。我们的目的是决定 LIPUS 处理怎么每天在造骨细胞影响了房间生存能力,碱的磷酸酶活动,成骨相关的基因表示,和使矿物化的小瘤形成。典型 osteoblastic 房间线 ROS 17/2.8 房间在 LIPUS 刺激的缺席或存在是有教养的。每日的 LIPUS 处理(1.5 MHz;20 min ) 在 30 mW/cm2 的紧张被管理 14 天。成骨相关的基因的表示用西方的弄污的分析在使用即时聚合酶链反应的 mRNA 层次并且在蛋白质层次被检验。LIPUS 刺激没影响房间生存能力的率。碱的磷酸酶活动与每日的 LIPUS 刺激在 10 天文化以后被增加。LIPUS 显著地增加了编码 Runx2, Msx2, Dlx5, osterix,骨头 sialoprotein,和骨头的 mRNAs 的表示形态基因的 protein-2,而它显著地减少了编码抄写因素 AJ18 的 mRNA 的表示。使矿物化的小瘤形成显著地在白天 14 LIPUS 刺激上被增加。LIPUS 刺激直接影响了 osteogenic 房间,导致使矿物化的小瘤形成。LIPUS 是可能的在牙槽的骨头在造骨细胞的关键功能的活动有基本影响。 | Akito Suzuki Tadahiro Takayama Naoto Suzuki Michitomo Sato Takeshi Fukuda Koichi Ito | 2009 | Acta Biochimica et Biophysica Sinica2009，41，2： | 23 |
| 3 | Confirming Stereochemical Structures of Strigolactones Produced by Rice and Tobacco显示文摘主要 strigolactones (SL ) 由米饭生产了(Oryza sativa L。cv。Nipponbare ) 并且烟草(Nicotiana tabacum L。cv。Michinoku 号码 1 ) 被净化,他们的 stereochemical 结构被在 ESILC/MS 和 GCMS 为他们的 NMR 和 CD 数据和保留时间和集体破碎与光学地纯的合成标准作比较决定。从米饭植物的根流出物净化的 SL 是 orobanchol, orobanchyl 醋酸盐,和 ent-2-epi-5-deoxystrigol。除了这些 SL, 7-oxoorobanchyl 醋酸盐并且通常认为三 methoxy-5-deoxystrigol 异构体被最小公倍数 /MS 检测。7-oxoorobanchyl 醋酸盐的生产似乎发生在早生长阶段,当它仅仅在在孵化的第一个星期期间收集的根流出物被检测。烟草的根流出物包含了至少 11 SL,包括其结构尚待被澄清的通常认为的 didehydro-orobanchol 的 solanacol, solanacyl 醋酸盐, orobanchol, ent-2-epi-orobanchol, orobanchyl 醋酸盐, ent-2-epi-orobanchyl 醋酸盐, 5-deoxystrigol, ent-2-epi-5-deoxystrigol,和三异构体。而且,二 sorgolactone 异构体然而并非 sorgolactone 被最小公倍数 /MS 分析作为次要的 SL 检测。注意米饭植物生产了仅仅 orobanchol 类型 SL 是吸引人的,源于 ent-2-epi-5-deoxystrigol,而是 orobanchol 类型和 strigol 类型 SL,源于 5-deoxystrigol 在烟草植物被检测。 | Xiaonan Xie Kaori Yoneyama Takaya Kisugi Kenichi Uchida Seisuke Ito Kohki Akiyama Hideo Hayashi Takao Yokota Takahito Nomura Koichi Yoneyama | 2013 | Molecular Plant2013，6，1： | 9 |
| 4 | Clinical diagnostic criteria of autoimmune pancreatitis: revised proposal显示文摘 | Kazuichi Okazaki Shigeyuki Kawa Terumi Kamisawa Satoru Naruse Shigeki Tanaka Isao Nishimori Hirotaka Ohara Tetsuhide Ito Seiki Kiriyama Kazuro Inui Tooru Shimosegawa Masaru Koizumi Koichi Suda Keiko Shiratori Koji Yamaguchi Taketo Yamaguchi Masanori Sugiy | 2006 | Journal of Gastroenterology2006，，7： | 3 |
| 5 | Targeting tight junctions during epithelial to mesenchymal transition in human pancreatic cancer显示文摘Pancreatic cancer continues to be a leading cause of cancer-related death worldwide and there is an urgent need to develop novel diagnostic and therapeutic strategies to reduce the mortality of patients with this disease. In pancreatic cancer, some tight junction proteins, including claudins, are abnormally regulated and therefore are promising molecular targets for diagnosis, prognosis and therapy. Claudin-4 and-18 are overexpressed in human pancreatic cancer and its precursor lesions. Claudin-4 is a high affinity receptor of Clostridium perfringens enterotoxin(CPE). The cytotoxic effects of CPE and monoclonal antibodies against claudin-4 are useful as novel therapeutic tools for pancreatic cancer. Claudin-18 could be a putative marker and therapeutic target with prognostic implications for patients with pancreatic cancer. Claudin-1,-7, tricellulin and marvelD3 are involved in epithelial to mesenchymal transition(EMT) of pancreatic cancer cells and thus might be useful as biomarkers during disease. Protein kinase C is closely related to EMT of pancreatic cancer and regulates tight junctions of normal human pancreatic duct epithelial cells and the cancer cells. This review focuses on the regulation of tight junctions via protein kinase C during EMT in human pancreatic cancer for the purpose of developing new diagnostic and therapeutic modalities for pancreatic cancer. | Daisuke Kyuno Hiroshi Yamaguchi Tatsuya Ito Tsuyoshi Kono Yasutoshi Kimura Masafumi Imamura Takumi Konno Koichi Hirata Norimasa Sawada Takashi Kojima | 2014 | World Journal of Gastroenterology2014，20，31： | 3 |
| 6 | Serum Mac-2 binding protein is a novel biomarker for chronic pancreatitis显示文摘AIM: To determine the efficacy of Mac-2 binding protein(Mac-2bp) for diagnosis of chronic pancreatitis.METHODS: Fifty-nine healthy volunteers(HV), 162 patients with chronic pancreatitis(CP), and 94 patients with pancreatic ductal adenocarcinoma(PDAC) were enrolled in this study. We measured serum Mac-2bp using our developed enzyme-linked immunosorbent assay kit. Additional biochemical variables were measured using an automated analyzer(including aminotransferase, alanine aminotransferase, γ-glutamyltransferase, alkaline phosphatase, triglyceride, C-reactive protein, and amylase levels) or chemiluminescent enzyme immunoassay(carbohydrate antigen 19-9 and carcinoembryonic antigen). The ability of Mac-2bp to predict CP diagnosis accurately was assessed using receiver operating characteristic(ROC) analyses.RESULTS: Serum Mac-2bp levels were significantly increased in CP patients compared to HV(P < 0.0001) and PDAC patients(P < 0.0001). Area under the ROC curve values of Mac-2bp for the discrimination of CP from HV and PDAC were 0.727 and 0.784, respectively. Multivariate analyses demonstrated that serum Mac-2bp levels were independent determinants for CP diagnosis from HV and PDAC patients. Immunohistological staining showed that Mac-2bp was expressed faintly in the pancreas tissues of both CP and PDAC patients. Serum aspartate aminotransferase, alanine aminotransferase, γ-glutamyltransferase, alkaline phosphatase, and triglyceride levels were significantly higher in patients with CP or PDAC. Serum Mac-2bp levels were highly correlated with protein levels of alanine aminotransferase, γ-glutamyltransferase, and C-reactive protein, but not amylase, suggesting that the damaged liver produces Mac-2bp. CONCLUSION: Measurement of serum Mac-2bp may be a novel and useful biomarker for CP diagnosis as well as liver fibrosis in the general population. | Tomohiro Maekawa Yoshihiro Kamada Yusuke Ebisutani Makiko Ueda Tomoki Hata Koichi Kawamoto Shinji Takamatsu Kayo Mizutani Mayuka Shimomura Tomoaki Sobajima Hironobu Fujii Kotarosumitomo Nakayama Kimihiro Nishino Makoto Yamada Takashi Kumada Toshifumi Ito Hidetoshi Eguchi Hiroaki Nagano Eiji Miyoshi | 2016 | World Journal of Gastroenterology2016，22，17： | 3 |
| 7 | Changes in portal venous pressure in the early phase after living donor liver transplantation: pathogenesis and clinical implications1,2显示文摘 | Takashi Ito Tetsuya Kiuchi Hidekazu Yamamoto Fumitaka Oike Yasuhiro Ogura Yasuhiro Fujimoto Kazuhiro Hirohashi and Koichi Tanaka | 2003 | Transplantation2003，，8： | 3 |
| 8 | Glicentin-containing cells in intestinal metaplasia, adenoma and carcinoma of the stomach显示文摘 | Hisao Ito Hiroshi Yokozaki Jotaro Hata Koichi Mandai Eiichi Tahara | 1984 | Virchows Archiv A Pathological Anatomy and Histopathology1984，，1： | 2 |
| 9 | A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour显示文摘 | Mika Ito Akitoshi Nakashima Takao Hidaka Motonori Okabe Nguyen Duy Bac Shihomi Ina Satoshi Yoneda Arihiro Shiozaki Shigeki Sumi Koichi Tsuneyama Toshio Nikaido Shigeru Saito | 2009 | Journal of Reproductive Immunology2009，，1： | 2 |
| 10 | mPGES-1 expression in non-cancerous liver tissue impacts on postoperative recurrence of HCC显示文摘AIM:To investigate whether microsomal prostaglandin E synthase-1 (mPGES-1) expression in hepatocellular carcinoma (HCC) and in non-cancerous liver affects HCC prognosis after hepatectomy. METHODS: The relationship between patient clinical prof iles, tumor factors, surgical determinants, and mPGES-1 expression and the recurrence-free survival rate were examined in 64 patients who underwent curative hepatectomy between March 2003 and December 2006. RESULTS: The scores for mPGES-1 expression were higher in well differentiated and moderately differentiated HCC tissues than in poorly differentiated HCC tissues (well differentiated, 5.1 ± 2.7; moderately differentiated, 5.1 ± 1.7; poorly differentiated, 3.0 ± 1.8). In noncancerous liver tissues, the mPGES-1 levels were higher in injured liver tissues than in normal tissues. Cirrhotic livers had higher mPGES-1 levels than livers with chronic hepatitis (normal livers, 3.3 ± 0.7; chronic hepatitic livers, 5.4 ± 1.9; cirrhotic livers, 6.4 ± 1.6). A univariate analysis revealed that the recurrence-free survival rate was signif icantly lower in patients with vascular invasion,a higher mPGES-1 level in non-cancerous liver tissue,a larger tumor diameter (≥5 cm), and a lower serum albumin level (≤3.7 g/dL). The mPGES-1 expression in HCC tissues did not correlate well with postoperative recurrence. A multivariate analysis demonstrated that the presence of vascular invasion and higher mPGES-1 levels were statistically significant independent predictors for early postoperative recurrence of HCC.CONCLUSION: Increased mPGES-1 expression in noncancerous liver tissues is closely associated with the early recurrence of HCC after curative resection. | Koichi Nonaka Hikaru Fujioka Yasushi Takii Seigo Abiru Kiyoshi Migita Masahiro Ito Takashi Kanematsu Hiromi Ishibashi | 2010 | World Journal of Gastroenterology2010，16，38： | 2 |
| 11 | The revised Japanese clinical diagnostic criteria for chronic pancreatitis显示文摘 | Tooru Shimosegawa Keisho Kataoka Terumi Kamisawa Hiroyuki Miyakawa Hirotaka Ohara Tetsuhide Ito Satoru Naruse Naohiro Sata Koichi Suda Morihisa Hirota Yoshifumi Takeyama Keiko Shiratori Takashi Hatori Makoto Otsuki Yutaka Atomi Kentaro Sugano Masao Tanaka | 2010 | Journal of Gastroenterology2010，，6： | 2 |
| 12 | Neonatal Exposure to Sevoflurane Causes Significant Suppression of Hippocampal Long-Term Potentiation in Postgrowth Rats显示文摘 | Rui Kato Kaori Tachibana Naoki Nishimoto Toshikazu Hashimoto Yosuke Uchida Ryoko Ito Kenkichi Tsuruga Koichi Takita Yuji Morimoto | 2013 | Anesthesia & Analgesia2013，，6： | 2 |
| 13 | Outcomes of Adult-to-Adult Living Donor Liver Transplantation: A Single Institution?s Experience With 335 Consecutive Cases显示文摘 | Daisuke Morioka Hiroto Egawa Mureo Kasahara Takashi Ito Hironori Haga Yasutsugu Takada Hiroshi Shimada Koichi Tanaka | 2007 | Annals of Surgery2007，，2： | 2 |
| 14 | Kidney GATA3^(+) regulatory T cells play roles in the convalescence stage after antibody-mediated renal injury显示文摘FoxP3^(+)regulatory T cells(Tregs)play crucial roles in peripheral immune tolerance.In addition,Tregs that reside or accumulate in nonlymphoid tissues,called tissue Tregs,exhibit tissue-specific functions and contribute to the maintenance of tissue homeostasis and repair.In an experimental mouse model of crescentic glomerulonephritis induced by an anti-glomerular basement membrane antibody,Tregs started to accumulate in the kidney on day 10 of disease onset and remained at high levels(~30–35%of CD4^(+)T cells)during the late stage(days 21–90),which correlated with stable disease control.Treg depletion on day 21 resulted in the relapse of renal dysfunction and an increase in Th1 cells,suggesting that Tregs are essential for disease control during the convalescence stage.The Tregs that accumulated in the kidney showed tissue Treg phenotypes,including high expression of GATA3,ST2(the IL33 receptor subunit),amphiregulin(Areg),and PPARγ.Although T-bet^(+) Tregs and RORγt^(+) Tregs were observed in the kidney,GATA3^(+) Tregs were predominant during the convalescence stage,and a PPARγagonist enhanced the accumulation of GATA3^(+) Tregs in the kidney.To understand the function of specific genes in kidney Tregs,we developed a novel T cell transfer system to T cell-deficient mice.This experiment demonstrates that ST2,Areg,and CCR4 in Tregs play important roles in the accumulation of GATA3^(+) Tregs in the kidney and in the amelioration of renal injury.Our data suggest that GATA3 is important for the recruitment of Tregs into the kidney,which is necessary for convalescence after renal tissue destruction. | Ryota Sakai Minako Ito Kyoko Komai Mana Iizuka-Koga Kazuhiko Matsuo Takashi Nakayama Osamu Yoshie Koichi Amano Hiroshi Nishimasu Osamu Nureki Masato Kubo Akihiko Yoshimura | 2021 | Cellular & Molecular Immunology2021，18，5： | 2 |
| 15 | Blocking of monocyte chemoattractant protein-1 (MCP-1) activity attenuates the severity of acute pancreatitis in rats显示文摘 | Toshiaki Ishibashi Haifeng Zhao Ken Kawabe Takamasa Oono Kensuke Egashira Koichi Suzuki Hajime Nawata Ryoichi Takayanagi Tetsuhide Ito | 2008 | Journal of Gastroenterology2008，，1： | 1 |
| 16 | Dispersion polymerization显示文摘 | Seigou Kawaguchi Koichi Ito | 2005 | Adv Polym Sci2005，175，： | 1 |
| 17 | BACKGROUND AND CLINICAL IMPACT OF TISSUE CONGESTION IN RIGHT-LOBE LIVING-DONOR LIVER GRAFTS: A MAGNETIC RESONANCE IMAGING STUDY显示文摘 | Hidekazu Yamamoto Yoji Maetani Tetsuya Kiuchi Takashi Ito Satoshi Kaihara Hiroto Egawa Kyo Itoh Yasuo Kamiyama Koichi Tanaka | 2003 | Transplantation2003，，1： | 1 |
| 18 | Synthesis of polymer-supported chiral N-sulfonylamino acids and their use in asymmetric diels-alder reaction of cyclopentadiene with methacrolein显示文摘 | Koichi Kamahori Shoichi Tada Koichi Ito | 1995 | Tetrahedron: Asymmetry1995，6，10： | 1 |
| 19 | Are animal models useful for studying human disc disorders/degeneration?显示文摘 | Mauro Alini Stephen M. Eisenstein Keita Ito Christopher Little A. Annette Kettler Koichi Masuda James Melrose Jim Ralphs Ian Stokes Hans Joachim Wilke | 2008 | European Spine Journal2008，，1： | 1 |
| 20 | A polysaccharide extracted from Grifola frondosa enhances the anti-tumor activity of bone marrow-derived dendritic cell-based immunotherapy against murine colon cancer显示文摘 | Yuki Masuda Koichi Ito Morichika Konishi Hiroaki Nanba | 2010 | Cancer Immunology, Immunotherapy2010，，10： | 1 |
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