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| 1 | Age-Triggered and Dark-Induced Leaf Senescence Require the bHLH Transcription Factors PIF3, 4, and 5显示文摘叶老朽能被很多发展、环境的因素触发并且支持。证据的众多的线在叶老朽的规定建议了 phytochromes 的参与,但是相关发信号小径和生理的机制糟糕被理解。在这研究,我们开始识别了交往 phytochrome 因素(程序信息文件) 叶老朽的 3, 4,和 5 个同样通常认为的调停人。PIF 基因的变化在被触发年龄、导致黑暗的老朽导致了显著地提高的叶长寿,而这些基因的 overexpressions 在 Arabidopsis 加速了被触发年龄、导致黑暗的老朽。一致地,稀释导致黑暗的 transcriptional 改变的 PIF4 的 loss-of-function 把产生与叶绿体恶化和反应的氧种类(ROS ) 联系了。ChIPPCR 和双酶的试金证明 PIF4 能激活叶绿素降级规章的基因 NYE1 并且镇压由到他们的倡导者区域的绑定的叶绿体活动维护者基因 GLK2。最后,导致黑暗的乙烯生合成和导致乙烯的老朽两个都在 pif4 被阻抑,建议在乙烯生合成和发信号的小径的 PIF4 的参与。我们的学习提供证据那 PIF3, 4,和 5 是进轻发信号的机制的卓见在叶老朽的规定包含了的新奇积极老朽调停人和获得。 | Yi Song Chuangwei Yang Shan Gao Wei Zhang Lin Li Benke Kuai | 2014 | Molecular Plant2014，7，12： | 20 |
| 2 | Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response显示文摘Personalized immunotherapy targeting tumor-specific antigens(TSAs)could generate efficient and safe antitumor immune response without damaging normal tissues.Although neoantigen vaccines have shown therapeutic effect in clinic trials,precise prediction of neoantigens from tumor mutations is still challenging.The host antitumor immune response selects and activates T cells recognizing tumor antigens.Hence,T cells engineered with T-cell receptors(TCRs)from these naturally occurring tumor antigen-specific T(Tas)cells in a patient will target personal TSAs in his/her tumor.To establish such a personalized TCR-T cell therapy,we comprehensively characterized T cells in tumor and its adjacent tissues by single-cell mRNA sequencing(scRNA-seq),TCR sequencing(TCR-seq)and in vitro neoantigen stimulation.Compared to bystander T cells circulating among tissues,Tas cells were characterized by tumor enrichment,tumor-specific clonal expansion and neoantigen specificity.We found that CXCL13 is a unique marker for both CD4^(+)and CD8^(+)Tas cells.Importantly,TCR-T cells expressing TCRs from Tas cells showed significant therapeutic effects on autologous patient-derived xenograft(PDX)tumors.Intratumoral Tas cell levels measured by CXCL13 expression precisely predicted the response to immune checkpoint blockade,indicating a critical role of Tas cells in the antitumor immunity.We further identified CD200 and ENTPD1 as surface markers for CD4^(^(+))and CD8^(^(+))Tas cells respectively,which enabled the isolation of Tas cells from tumor by Fluorescence Activating Cell Sorter(FACS)sorting.Overall,our results suggest that TCR-T cells engineered with Tas TCRs are a promising agent for personalized immunotherapy,and intratumoral Tas cell levels determine the response to immunotherapy. | Jingjing He Xinxin Xiong Han Yang Dandan Li Xuefei Liu Shuo Li Shuangye Liao Siyu Chen Xizhi Wen Kuai Yu Lingyi Fu Xingjun Dong Kaiyu Zhu Xiaojun Xia Tiebang Kang Chaochao Bian Xiang Li Haiping Liu Peirong Ding Xiaoshi Zhang Zhenjiang Liu Wende Li Zhixiang Zuo Penghui Zhou | 2022 | Cell Research2022，32，6： | 10 |
| 3 | Mitochondrial uncoupling protein 2 expression in colon cancer and its clinical significance显示文摘AIM: To detect the expression of mitochondrial uncoupling protein 2 (UCP2) in colon cancer and analyze the relation between UCP2 expression and clinical pathological features of colon cancer.METHODS: Fifteen colon tissue samples and 15 its adjacent tissue samples were obtained from colon cancer patients during surgical interventions. UCP2 expression was detected with immunohistochemical method in 10 normal controls, 10 hyperplastic polyp patients, 20 tubular adenoma patients and 78 colon cancer patients. Patients with rectal cancer were excluded. Quantitative reverse transcription polymerase chain reaction and Western blotting were used to detect UCP2 expressions in colon cancer tissue samples and its adjacent tissue samples. Relation between UCP2 expression and clinical pathological features of colon cancer was also analyzed. RESULTS: The UCP2 mRNA expression level was fourfold higher in colon cancer tissue samples than in its adjacent tissue samples. The UCP2 protein expression level was three-fold higher in colon cancer tissue samples than in its adjacent normal tissue samples. The UCP2 was mainly expressed in cytoplasm. The UCP2 was not expressed in normal colon mucosa. Strong positive staining for UCP2 with a diffuse distribution pattern was identified throughout the mucosa in colon cancer tissue samples with a positive expression rate of 85.9%. The UCP2 expression level was higher in colon cancer tissue samples at clinical stages Ⅲ and Ⅳ than in those at stageⅠ+ Ⅱ. Univariate analysis showed that the high UCP2 expression level was significantly correlated to colon cancer metastasis (hazard ratio = 4.321, confidence interval = 0.035-0.682, P = 0.046). CONCLUSION: UCP2 is highly expressed in human colon cancer tissue and may be involved in colon cancer metastasis. | Xiao-Yi Kuai, Ze-Yu Ji, Hong-Jie Zhang,Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China | 2010 | World Journal of Gastroenterology2010，16，45： | 9 |
| 4 | Reverse Genetic Identification of CRN1 and its Distinctive Role in Chlorophyll Degradation in Arabidopsis显示文摘Recent identification of NYE1/SGR1 brought up a new era for the exploration of the regulatory mechanism of Chlorophyll (Chl) degradation.Cluster analysis of senescence associated genes with putative chloroplast targeting sequences revealed several genes sharing a similar expression pattern with NYE1.Further characterization of available T-DNA insertion lines led to the discovery of a novel stay-green gene CRN1 (Co-regulated with NYE1).Chl breakdown was significantly restrained in crn1-1 under diversified senescence scenarios,which is comparable with that in acd1-20,but much more severe than that in nye11.Notably,various Chl binding proteins,especially trimeric LHCP II,were markedly retained in crn1-1 four days after dark-treatment,possibly due to a lesion in disassociation of protein-pigment complex.Nevertheless,the photochemical efficiency of PSII in crn1-1 declined,even more rapidly,two days after dark-treatment,compared to those in Col-0 and nye1-1.Our results suggest that CRN1 plays a crucial role in Chl degradation,and that loss of its function produces various side-effects,including those on the breakdown of Ch-protein complex and the maintenance of the residual photosynthetic capability during leaf senescence. | Guodong Ren Qian Zhou Shouxin Wu Yufan Zhang Lingang Zhang Jirong Huang Zhenfei Sun Benke Kuai | 2010 | Journal of Integrative Plant Biology2010，52，5： | 8 |
| 5 | NON-YELLOWING2 (NYE2), a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis显示文摘 | Wu, Shouxin Li, Zhongpeng Yang, Lifeng Xie, Zuokun Chen, Junyi Zhang, Wei Liu, Tianqi Gao, Shan Gao, Jiong Zhu, Yihua Xin, Jiwen Ren, Guodong Kuai, Benke | 2016 | Molecular Plant2016，9，4： | 6 |
| 6 | Protective effect of oxysophoridine on cerebral ischemia/reperfusion injury in mice显示文摘Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase. Protein and mRNA expression levels of N-methyl-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyl-D-aspartate receptor subunit NR1. | Hongbo Wang Yuxiang Li Ning Jiang Xiaoping Chen Yi Zhang Kuai Zhang Tengfei Wang Yinju Hao Lin Ma Chengjun Zhao Yanrong Wang Tao Sun Jianqiang Yu | 2013 | Neural Regeneration Research2013，8，15： | 5 |
| 7 | The NPR1-WRKY46-WRKY6 signaling cascade mediates probenazole/salicylic acid-elicited leaf senescence in Arabidopsis thaliana显示文摘Endogenous salicylic acid(SA) regulates leaf senescence, but the underlying mechanism remains largely unexplored. The exogenous application of SA to living plants is not efficient for inducing leaf senescence. By taking advantage of probenazole(PBZ)-inducedbiosynthesisof endogenous SA, we previously established a chemical inducible leaf senescence system that depends on SA biosynthesis and its core signaling receptor NPR1 in Arabidopsis thaliana. Here,using this system, we identified WRKY46 and WRKY6 as key components of the transcriptional machinery downstream of NPR1 signaling. Upon PBZ treatment, the wrky46 mutant exhibited significantly delayed leaf senescence. We demonstrate that NPR1 is essential for PBZ/SA-induced WRKY46 activation, whereas WRKY46 in turn enhances NPR1 expression. WRKY46 interacts with NPR1 in the nucleus, binding to the W-box of the WRKY6 promoter to induce its expression in response to SA signaling. Dysfunction of WRKY6 abolished PBZ-induced leaf senescence, while overexpression of WRKY6 was sufficient to accelerate leaf senescence even under normal growth conditions, suggesting that WRKY6 may serve as an integration node of multiple leaf senescence signaling pathways. Taken together,these findings reveal that the NPR1-WRKY46-WRKY6 signaling cascade plays a critical role in PBZ/SA-mediated leaf senescence in Arabidopsis. | Dingyu Zhang Zheng Zhu Jiong Gao Xin Zhou Shuai Zhu Xiaoyan Wang Xiaolei Wang Guodong Ren Benke Kuai | 2021 | Journal of Integrative Plant Biology2021，63，5： | 3 |
| 8 | Lamin-like Proteins Negatively Regulate Plant Immunity through NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 and NONEXPRESSOR OF PR 3ENES1 in Arabidopsis thaliana显示文摘 | Tongtong Guo Xuegao Mao Hui Zhang Yu Zhang Mengdi Fu Zhenfei Sun Peng Kuai Yonggen Lou Yuda Fang | 2017 | Molecular Plant2017，10，10： | 3 |
| 9 | Epidermal growth factor receptor-targeted immune magnetic liposomes capture circulating colorectal tumor cells efficiently显示文摘AIM To compare the capacity of newly developed epidermal growth factor receptor(EGFR)-targeted immune magnetic liposomes(EILs) vs epithelial cell adhesion molecule(Ep CAM) immunomagnetic beads to capture colorectal circulating tumor cells(CTCs).METHODS EILs were prepared using a two-step method, and the magnetic and surface characteristics were confirmed. The efficiency of capturing colorectal CTCs as well as the specificity were compared between EILs and Ep CAM magnetic beads. RESULTS The obtained EILs had a lipid nanoparticle structure similar to cell membrane. Improved binding with cancer cells was seen in EILs compared with the method of coupling nano/microspheres with antibody. The binding increased as the contact time extended. Compared with Ep CAM immunomagnetic beads, EILs captured more CTCs in peripheral blood from colorectal cancer patients. The captured cells showed consistency with clinical diagnosis and pathology. Mutation analysis showed same results between captured CTCs and cancer tissues. CONCLUSION EGFR antibody-coated magnetic liposomes show high efficiency and specificity in capturing colorectal CTCs. | Jing-Hua Kuai Qing Wang Ai-Jun Zhang Jing-Yu Zhang Zheng-Feng Chen Kang-Kang Wu Xiao-Zhen Hu | 2018 | World Journal of Gastroenterology2018，24，3： | 2 |
| 10 | Exploring novel bioactive compounds from marine microbes显示文摘 | Lixin Zhang Rong An Jinping Wang Nuo Sun Si Zhang Jiangchun Hu Jun Kuai | 2005 | Current Opinion in Microbiology2005，，3： | 2 |
| 11 | Hematite hollow spheres with a mesoporous shell: Controlled synthesis and applications in gas sensor and lithium ion batteries显示文摘 | WU Zhengcui YU Kuai ZHANG Shudong | 2008 | J Phys Chem C2008，112，11： | 1 |
| 12 | Reactivation of the homeotic tumorsuppressor gene CDX2 by 5- aza2'- deoxycytidine- induceddemethylation inhibits cell proliferation and induces caspaseindependentapoptosisingastriccancercells显示文摘 | Zhang JF Zhang JG Kuai XL | 2013 | ExpTherMed2013，5，3： | 1 |
| 13 | Liposome formulated with TAT-modified cholesterol for enhancing the brain delivery显示文摘 | Yao Qin Huali Chen Wenmin Yuan Rui Kuai Qianyu Zhang Fulan Xie Li Zhang Zhirong Zhang Ji Liu Qin He | 2011 | International Journal of Pharmaceutics2011，，1： | 1 |
| 14 | microRNA -32 inhibits the pro- liferation and invasion of the SGC -7901 gastric cancer cell line in vitro显示文摘 | Zhang J Kuai X Song M | 2014 | Oncol Lett2014，7，1： | 1 |
| 15 | Liposome formulated with TAT-modified cholesterol for enhancing the brain delivery显示文摘 | Yao Qin Huali Chen Wenmin Yuan Rui Kuai Qianyu Zhang Fulan Xie Li Zhang Zhirong Zhang Ji Liu Qin He | 2011 | International Journal of Pharmaceutics2011，，1： | 1 |
| 16 | Experimental analysis of tensile behaviors of polytetrafluoroethylene-coated fabrics subjected to monotonous and cyclic loading显示文摘 | ZHANG Ying-ying ZHANG Qi-lin LEI Ke KUAI Bei-lei | 2014 | Textile Research Journal2014，84，3： | 1 |
| 17 | Liposome formulated with TAT-modified cholesterol for improving brain delivery and therapeutic efficacy on brain glioma in animals显示文摘 | Yao Qin Huali Chen Qianyu Zhang Xiaoxiao Wang Wenmin Yuan Rui Kuai Jie Tang Li Zhang Zhirong Zhang Qiang Zhang Ji Liu Qin He | 2011 | International Journal of Pharmaceutics2011，，2： | 1 |
| 18 | Liposome formulated with TAT-modified cholesterol for improving brain delivery and therapeutic efficacy on brain glioma in animals显示文摘 | Qin Y Chen H Zhang Q Wang X Yuan W Kuai R | 2011 | Int J Pharm2011，420，2： | 1 |
| 19 | Internet traffic behavior profiling for network security monitoring显示文摘 | Kuai Xu Zhang Zhi-li and Bhattacharyya S | 2008 | IEEE/ACM Transactions on Networking2008，16，6： | 1 |
| 20 | Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase Ⅱ stage显示文摘Aim:Protein kinase C(PKC)is as a family of serine/threonine kinases that can beactivated by Ca^(2+),phospholipid and diacylglycerol.PKC plays an important rolein oocyte maturation and activation.This study was undertaken to investigateclassical PKC(cPKC)in human oocyte maturation and activation.Methods:Ger-minal vesicle(GV)and metaphase Ⅱ(MⅡ)stage oocytes were collected fromhealthy women.The expression and distribution of cPKC were investigated byimmunoflourescence.MⅡ oocytes were treated with PKC activator or inhibitorand imaged using a laser confocal scanning microscope(LCSM).Results:In GVoocytes,PKC α,β1 and γ were localized to the germinal vesicles,with a weakexpression in ooplasm.In MⅡ oocytes,PKCα,β1 and γ were distributed evenly inooplasm.After treatment with PKC activator,phorbol 12-myristate 13-acetate(PMA),cPKC translocated to the periphery of oocyte,and cortical granules(CG)exocytosis was found.When the oocytes were treated with PKC inhibitor,staurosporine,no translocation of cPKC and CG exocytosis were found.Conclusion:PKCα,β1 and γ exist in human oocytes and activation of these sub-units could induce CG exocytosis in MⅡ stage. | Xue-qing WU Xiao ZHANG Xiao-hong LI Hui-hong CHENG Yan-rong KUAI Song WANG Ying-lu GUO | 2006 | Acta Pharmacologica Sinica2006，27，10： | 1 |
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