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| 1 | Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor显示文摘T 房间 receptor-CD3 建筑群(TCR ) 是能基于 CD3 细胞质的领域的各种各样的生物化学的变化开始抗原特定的有免疫力的回答的一台万用的发信号机器,但是内在的结构的基础留下逃犯。这里,我们开发了生物物理的途径学习 CD3 细胞质的领域的 conformational 动力学(CD3 CD ) 。在单个分子的水平,我们发现那 CD3 CD 能与三个功能的主题的不同坦诚有多重 conformational 状态,即, ITAM, BRS 和 PRS。因为,这些符合构造被产生 CD3 CD 有异构的类脂化合物绑定性质因此有异构的动力学。实时房间的成像实验证明不同抗原刺激能稳定 CD3 在不同符合构造的 CD 。类脂化合物依赖者 conformational 动力学因此为 TCR 的万用的发信号性质提供结构的基础。 | Xingdong Guo Chengsong Yan Hua Li Wenmao Huan Xiaoshan Shi Min Huang Yingfang Wang Weiling Pan Mingjun Cai Lunyi Li Wei Wu Yibing Bai Chi Zhang Zhijun Liu Xinyan Wang Xiaohui F Zhang Chun Tang Hongda Wang Wanli Liu Bo Ouyang Catherine C Wong Yi Cao Chenqi Xu | 2017 | Cell Research2017，27，4： | 5 |
| 2 | A novel gatifloxacin-loaded intraocular lens for prophylaxis of postoperative endophthalmitis显示文摘Postoperative endophthalmitis(POE)has been the most threatening complication after cataract surgery,which perhaps can be solved by the antibiotic-loaded intraocular lens(IOL).However,most drug-loaded IOLs demonstrate insufficient drug quantity,short release time,increased implantation-related difficulties or other noticeable drawbacks.To prevent POE and to address these deficiencies,a drug-loaded copolymer IOL,prepared from poly(urethane acrylate)prepolymer,isobornyl methacrylate(IBOMA),N-vinyl-2-pyrrolidone(NVP),Irgacure 819,RUVA-93,and gatifloxacin(GAT),was rapidly fabricated via photocuring and by using a 3D-printed mold.This composite displayed an outstanding and controllable GAT release behavior in vitro,a high light transmittance,and a moderate refractive index.Also,it demonstrated improved strain stress and elongation compared with the reference commercial acrylic IOL material.In vivo tests demonstrated satisfying released drug concentration at the early treatment stage.In vitro and in vivo studies further confirmed the remarkable bacterial inhibition and prevention of POE by the proposed IOL,which also displayed good biocompatibility.These findings suggested that the GAT-loaded IOL could be a promising implant to prevent and cure POE,also the proposed methods could inspire more designs for various medical applications. | Mengna Li Jing-Wei Xu Jiayong Li Wei Wang Chenqi Luo Haijie Han Zhi-Kang Xu Ke Yao | 2023 | Bioactive Materials2023，，2： | 2 |
| 3 | Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness显示文摘Tonic signaling of chimeric antigen receptor(CAR),i.e.,the spontaneous CAR activation in the absence of tumor antigen stimulation,is considered to be a pivotal event controlling CAR-T efficacy.However,the molecular mechanism underlying the spontaneous CAR signals remains elusive.Here,we unveil that positively charged patches(PCPs)on the surface of the CAR antigenbinding domain mediate CAR clustering and result in CAR tonic signaling.For CARs with high tonic signaling(e.g.,GD2.CAR and CSPG4.CAR),reducing PCPs on CARs or boosting ionic strength in the culture medium during ex vivo CAR-T cell expansion minimizes spontaneous CAR activation and alleviates CAR-T cell exhaustion.In contrast,introducing PCPs into the CAR with weak tonic signaling,such as CD19.CAR,results in improved in vivo persistence and superior antitumor function.These results demonstrate that CAR tonic signaling is induced and maintained by PCP-mediated CAR clustering.Notably,the mutations we generated to alter the PCPs maintain the antigen-binding affinity and specificity of the CAR.Therefore,our findings suggest that the rational tuning of PCPs to optimize tonic signaling and in vivo fitness of CAR-T cells is a promising design strategy for the nextgeneration CAR. | Jian Chen Shizhen Qiu Wentao Li Kun Wang Yu Zhang Han Yang Baichuan Liu Guangfei Li Li Li Min Chen Junjie Lan Jiahua Niu Peijie He Lei Cheng Gaofeng Fan Xin Liu Xianmin Song Chenqi Xu Haitao Wu Haopeng Wang | 2023 | Cell Research2023，33，5： | 2 |
| 4 | A new oil spill detection algorithm based on Dempster-Shafer evidence theory显示文摘Features of oil spills and look-alikes in polarimetric synthetic aperture radar(SAR)images always play an important role in oil spill detection.Many oil spill detection algorithms have been implemented based on these features.Although environmental factors such as wind speed are important to distinguish oil spills and look-alikes,some oil spill detection algorithms do not consider the environmental factors.To distinguish oil spills and look-alikes more accurately based on environmental factors and image features,a new oil spill detection algorithm based on Dempster-Shafer evidence theory was proposed.The process of oil spill detection taking account of environmental factors was modeled using the subjective Bayesian model.The Faster-region convolutional neural networks(RCNN)model was used for oil spill detection based on the convolution features.The detection results of the two models were fused at decision level using Dempster-Shafer evidence theory.The establishment and test of the proposed algorithm were completed based on our oil spill and look-alike sample database that contains 1798 image samples and environmental information records related to the image samples.The analysis and evaluation of the proposed algorithm shows a good ability to detect oil spills at a higher detection rate,with an identifi cation rate greater than 75%and a false alarm rate lower than 19%from experiments.A total of 12 oil spill SAR images were collected for the validation and evaluation of the proposed algorithm.The evaluation result shows that the proposed algorithm has a good performance on detecting oil spills with an overall detection rate greater than 70%. | Tianlong ZHANG Jie GUO Chenqi XU Xi ZHANG Chuanyuan WANG Baoquan LI | 2022 | Journal of Oceanology and Limnology2022，40，2： | 1 |
| 5 | Structural understanding of T cell receptor triggering显示文摘The T cell receptor(TCR)is one of the most complicated receptors in mammalian cells,and its triggering mechanism remains mysterious.As an octamer complex,TCR comprises an antigen-binding subunit(TCRαβ)and three CD3 signaling subunits(CD3ζζ,CD3δε,and CD3γε).Engagement of TCRαβwith an antigen peptide presented on the MHC leads to tyrosine phosphorylation of the immunoreceptor tyrosine-based activation motif(ITAM)in CD3 cytoplasmic domains(CDs),thus translating extracellular binding kinetics to intracellular signaling events.Whether conformational change plays an important role in the transmembrane signal transduction of TCR is under debate.Attracted by the complexity and functional importance of TCR,many groups have been studying TCR structure and triggering for decades using diverse biochemical and biophysical tools.Here,we synthesize these structural studies and discuss the relevance of the conformational change model in TCR triggering. | Xinyi Xu Hua Li Chenqi Xu | 2020 | Cellular & Molecular Immunology2020，17，3： | 1 |
| 6 | Phase and structure modulating of bimetallic Cu/In nanoparticles realizes efficient electrosynthesis of syngas with wide CO/H_(2) ratios显示文摘Syngas(CO+H_(2))is the incredibly important feedstock for producing synthetic fuels and various value-added chemicals.CO_(2) electrochemical reduction to syngas is an environmental-friendly and sustainable approach,but still challenging to produce tunable syngas with a wide ratio of CO+H_(2).Herein,by modulating the structure and phase,we have successfully obtained a series of copper-indium(Cu-ln)catalysts,which are efficient for producing syngas with tunable CO+H_(2) ratios.A series of Culn bimetallic catalysts with different structures from hollow sphere to two-layer hollow sphere and different phases from CuO to CU_(2)O are developed.We find that the CO and H2 are the only gaseous products,in which the CCD/H_(2) ratios can be readily tuned from 1.2±0.1 to 9.0±1.5 by simply controlling the thermal annealing temperature.It also exhibits high durability during a 10-h test.The unique performance is attributed to the modulated In enrichment on the Cu surfaces during the CO_(2) reduction reaction,which causes the differences in binding energies for key reaction intermediates,thus resulting in the tunable composition of syngas.The present work emphasizes a simple yet efficient phase and structure modulating strategy for designing potential electrocatalysts for producing syngas with widely tunable CO+H_(2) ratios. | Chenqi Shen Pengtang Wang Leigang Li Xiaoqing Huang Qi Shao | 2022 | Nano Research2022，15，1： | 0 |
| 7 | Environmental heterogeneity regulates species-area relationships through the spatial distribution of species显示文摘Species-area relationships(SARs),also known as species-area curves,are fundamental scaling tools for biodiversity research.Sampling design and taxonomic groups affect the widely cited forms of species-area curves.However,the influence of sampling design and related environmental heterogeneity on SAR curves is rarely considered.Here,we investigated the SAR among different plant life forms(herbaceous plants,shrubs,and trees)in a 25.2-ha ForestGEO plot,the Wanglang Plot,in Sichuan,southwestern China,using a non-contiguous quadrat sampling method and power-law model.We compared the estimated parameters(the intercept c and the slope z)of the power-law models among different plant life forms,tested whether the SAR curve forms varied with sampling starting location,and assessed the effect of environmental heterogeneity accumulating with sampling area on curve variation.We found a wider range of variations in the SARs.The estimated c,z-values of power SAR were higher for the herbaceous plants than for the woody plants.A wider variation of SARs for the herbaceous plants than those for the woody plants.The selection of sampling starting location affected the SAR curve forms because of the roles of soil and topographic heterogeneity.We concluded that environmental heterogeneity regulates SAR curves sampled from different starting locations through spatial distribution of plant life forms.Thus,we recommend considering the design of sampling starting location when constructing SAR curves,especially in a heterogeneous habitat with unrandom distribution patterns of species. | Chenqi He Leqi Fang Xinyu Xiong Fan Fan Yangang Li Luoshu He Xiaoli Shen Sheng Li Chengjun Ji Jiangling Zhu | 2022 | Forest Ecosystems2022，9，3： | 0 |
| 8 | 表观遗传调节因子Uhrf1通过Akt-mTOR信号通路调控iNKT细胞的存活和分化显示文摘表观遗传调节因子Uhrf1(也被称作Np95)是一个DNA甲基化和组蛋白泛素化的调控因子,在胚胎的发育及肿瘤的发生中扮演重要角色。 | yu cui xufeng chen jiali zhang xiang sun haifeng liu li bai chenqi xu 刘小龙 | 2017 | 科学新闻2017，0，4： | 0 |
| 9 | Regulation of EGFR nanocluster formation by ionic protein-lipid interaction显示文摘表皮的生长因素受体(EGFR ) 的反常激活强烈与许多人的癌症被联系,但是内在的分子的机制充分没被理解。由使用直接随机的光重建显微镜学(dSTORM ) ,我们发现 EGFR 蛋白质在正常的肺的房间膜形成 nanoclusters 簇的上皮的房间和肺癌症房间,而是数字和尺寸显著地在肺癌症房间增加。EGFR 簇的形成被在 anionic 类脂化合物 phosphatidylinositol-4,5-bisphosphate (PIP2 ) 之间的离子的相互作用在血浆膜和 juxtamembrane (JM ) 调停 EGFR 的区域。由 PIP2 弄空或 JM 区域变化聚类的 EGFR 的混乱损害 EGFR 激活并且下游的发信号。而且,在组成地活跃的 EGFR 异种的 JM 区域变化稀释它房间转变的能力。一起,我们的调查结果在 EGFR 发信号和功能加亮 anionic phospholipids 的关键角色,并且揭示新奇机制在癌症解释 EGFR 的异常激活。 | Ye Wang Jing Gao Xingdong Guo Ti Tong Xiaoshan Shi Lunyi Li Miao Qi Yajie Wang Mingjun Cai Junguang Jiang Chenqi Xu Hongbin Ji Hongda Wang | 2014 | Cell Research2014，24，8： | 0 |
| 10 | Intrinsic-trap-regulating growth of clean graphene on high-entropy alloy substrate显示文摘A facile way to grow few-layer graphene on high-entropy alloy sheets is presented in this work.We systematically investigate the growth mechanism of graphene using the unique properties of FeCoNiCu_(0.25)high-entropy alloys.The intrinsic-trap-regulating growth mechanism derives from the synergistic effect of the multi-metal atoms and sluggish diffusion of high-entropy alloy.As a result,as-obtained few-layer of graphene has the characteristics of wide coverage,large size,good continuity,and high crystallinity with less amorphous carbon and extra wrinkles.Factors such as the Cu content,annealing time,growth temperature,growth time,carbon source flow rate,hydrogen flow rate and heat treatment method play a key role in the growth of high-quality graphene,and the best growth parameters have been explored.Besides,increasing alloy entropy is found to be responsible for the formation of high-quality graphene. | Xiaofei Hu Jiongzhao Li Zhe Wang Xudong Qian Chenqi Zhu Xiaogang Peng | 2022 | Nano Research2022，15，5： | 0 |
| 11 | A special collection of reviews on frontiers in immunology显示文摘During this challenging period in human history when SARS-CoV-2 has infected more than 20 million people without any sign of an end in sight,we have never been so in need of a healthy immune system to shield ourselves against this highly infectious virus.The reality is that we are still lacking in immunological knowledge to quickly develop therapeutics,either a drug or a vaccine,to slow down the spread of the virus.However,past immunological research has successfully rewarded human society with various immunotherapies,such as the immune checkpoint blockade therapies that have treated numerous cancer patients.The immune system,which intersects with virtually every system in the human body,lies at the center of health and disease.The complexity of the human immune system reflects an ancient Chinese philosophy known as“Yin and Yang”.Immune function needs to be robust yet restrained to efficiently clean up external threats without causing self-damage.When there are“positive”types of immune cells,“negative”types of immune cells must exist simultaneously.At a molecular level,stimulatory and inhibitory molecules reciprocally interplay to elicit well-regulated immune responses.To understand the dynamic and complicated immune responses,immunologists have stepped out of their comfort zones to absorb knowledge from other fields and to apply the newest technologies in their research.Many new branches of immunology have emerged in recent years,including but not limited to,cancer immunology,immunometabolism,microbiota and immunity,neuroimmunology. | Chenqi Xu Hua-Bing Li Richard A.Flavell | 2020 | Cell Research2020，30，10： | 0 |
| 12 | Behavioral and Physiological Responses of Sea Slug (Onchidium reevesii) to Low-Frequency Noise显示文摘A large amount of marine noise pollution from coastal industrial construction and the water transportation industry affects the growth and development of marine life,which is a big issue threatening marine organisms.However,most studies have focused on vertebrates,such as marine mammals and fishes,and little is known about the effects of noise on invertebrates.Therefore,the impacts of low-frequency noise(100,300,and 500 Hz)on behavioral and physiological responsesof the sea slug(Onchidium reevesii)were investigated.Under laboratory conditions,sea slugs were stimulated with low-frequency noise at 100,300,and 500 Hz for 1 h.Then,hemolymph enzyme activities(glucose,albumin,triglycerides,superoxide dismutase,catalase,and malondialdehyde)were measured,and mRNA expression of the hsp70 gene was detected in hemolymph and the nervous system by quantitative realtime polymerase chain reaction(qRT-PCR)analysis,while expression of the hsp70 protein was analyzed by immunohistochemistry.The results demonstrated that sea slugs were away from the stimulus source under the influence of low-frequency noise compared to the control group.Enzyme activities,as well as hsp70 gene mRNA and protein expression levels,were significantly higher in the noiseexposed groups than those in the control group.Overall,these changes indicate that low-frequency noise caused oxidative stress in sea slugs in vivo,and the oxidative damage gradually increased when the noise frequency was increased from 100 to 500 Hz. | TU Zhihan LI Chenqi JIA Jingjing SHEN Heding | 2022 | Journal of Ocean University of China2022，21，6： | 0 |
| 13 | Temperature-modulated inversion and switching of chiroptical responses in dynamic side-by-side oligomers of gold nanorods显示文摘Herein,a new strategy is proposed for achieving dynamic chiral controls in self-assembly systems of plasmonic nanorods based on temperature-modulation.Via enlarging Au{100}side facets of Au nanorod(AuNR)building block and changing surface ligand from often-used cetyltrimethylammonium bromide(CTAB)to cetylpyridinium chloride(CPC),inversion of chiroptical signal in side-by-side(SS)oligomers is realized.Under the guide of chiral cysteine(Cys),Au{100}side facet-linked SS rods twist in the opposite direction compared with Au{110}side facet-linked counterparts.At high CPC concentration,by controlling the incubation temperature of chiral Cys,the dominant twist mode can be regulated.Finite-difference time-domain(FDTD)simulations indicate the key role of the twisting dihedral angle of the oligomers in driving chiral signal inversion.At low CPC concentration,a temperature-sensitive chiral switching is observed owing to the conformation change of the CPC ligand layer.The temperature-modulated chiral responses are based on the interactions of chiral molecules,achiral surface ligands,and exposed facets of the building block.The rich dynamic tunability of chiroptical responses of plasmonic assemblies may find applications in stimulus-responsive nanodevices. | Hanbo Li Dejing Meng Chenqi Zhang Yinglu Ji Xinshuang Gao Zhijian Hu Xiaochun Wu | 2023 | Nano Research2023，16，12： | 0 |
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