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| 1 | Wnt/β-catenin signalling:function,biological mechanisms,and therapeutic opportunities显示文摘The Wnt/β-catenin pathway comprises a family of proteins that play critical roles in embryonic development and adult tissue homeostasis.The deregulation of Wnt/β-catenin signalling often leads to various serious diseases,including cancer and non-cancer diseases.Although many articles have reviewed Wnt/β-catenin from various aspects,a systematic review encompassing the origin,composition,function,and clinical trials of the Wnt/β-catenin signalling pathway in tumour and diseases is lacking.In this article,we comprehensively review the Wnt/β-catenin pathway from the above five aspects in combination with the latest research.Finally,we propose challenges and opportunities for the development of small-molecular compounds targeting the Wnt signalling pathway in disease treatment. | Jiaqi Liu Qing Xiao Jiani Xiao Chenxi Niu Yuanyuan Li Xiaojun Zhang Zhengwei Zhou Guang Shu Gang Yin | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 28 |
| 2 | Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains显示文摘在由编码 transposase 定序的小猪用绳子拖的平底渡船向量和助手 plasmidcontaining 的混合物的微注射编码基因进蚕鸡蛋的提高的绿荧光蛋白质(EGFP ) 的介绍以后比较的蚕 Bombyx moriwas 的三不同紧张的转变率。尽管在在在微注射鸡蛋(P=0.1258 ) 的肥沃的蛾的百分比的三紧张之中没有重要差别, G0 的百分比转变了在肥沃的蛾和注射鸡蛋的蛾两个都是显著地不同的(P=0.01368 和 P=0.02398,分别地) 。Nistari 紧张(印度紧张) 的转变率比另外的二紧张, Golden-yellow-cocoon (Vietnamesestrain ) 和 Jiaqiu (中国紧张) 的显著地高,它有类似的率。这些结果显示基于 piggyBac 的系统的转变效率可能与不同基因背景与蚕紧张变化。内长的象 piggyBac 一样元素的存在可能是影响介绍 piggyBac-derivedvectors,和多样的数量的转变效率的一个重要因素,在不同的蚕紧张的激活可能说明重要差别。 | Boxiong ZHONG~1 Jianying LI~1 Jin’e CHEN~(1,2) Jian YE~1 and Songdong YU~1 ~1College of Animal Sciences,Zhejiang University,Hangzhou 310029,China ~2Institute of Sericultural Research,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 17 |
| 3 | Geochemical Characteristics of the Upper Triassic Xujiahe Formation in Sichuan Basin, China and its Significance for Hydrocarbon Accumulation显示文摘The alternative development of coal-bearing hydrocarbon source rocks and low-porosity and low-permeability tight sandstone reservoirs of the Triassic Xujiahe Formation in the Sichuan Basin is favorable for near-source hydrocarbon accumulation. The natural gas composition of the Xujiahe Formation in the Sichuan Basin is dominated by hydrocarbon gases, of which the methane content is80.16%-98.67%. Typically, the C_2^+ content is larger than 5% in main wet gas. The dry gas is mainly distributed in the western and northern regions of the basin. The non-hydrocarbon gases mainly contain nitrogen, carbon dioxide, hydrogen, and helium, with a total content of <2%. The carbon isotope ranges of methane and its homologues in natural gas are: δ^(13)C_1 of-43.8‰ to-29.6‰, δ^(13)C_2 of-35.4‰ to-21.5‰, δ^(13)C_3 of-27.6‰ to-19.8‰,and δ^(13)C_4 of-27.7‰ to-18.8‰. δ^(13)C_3>δ^(13)C_4 occurs in some natural gas with a low evolution degree; such gas is mainly coal-related gas from humic-type source rocks of the Xujiahe Formation. As for the natural gas, δ~2 H_(CH4) values ranged from-195‰ to-161‰,δ~2 H_(C2H6) values ranged from-154‰ to-120‰, and δ~2 H_(C3H8) values ranged from-151‰ to-108‰. The dry coefficient,δ^(13)C and δ~2 H_(CH4) are all positively correlated with the maturity of source rocks. The higher the maturity of source rocks is, the larger the natural gas dry coefficient is and the larger the δ^(13)C and δ~2 H_(CH_4) values are, indicative of the characteristic of near-source accumulation. The δ~2 H_(C2H6) value of natural gas is influenced by paleosalinity to a relatively large extent; the higher the paleosalinity is, the larger the δ~2 H_(C2H6) value is. The Pr/Ph value of the condensate oil ranged from 1.60 to 3.43, illustrating light oxidization-light reduction and partial-oxidization characteristics of the depositional environment of coal-bearing source rocks of the Xujiahe Formation. The natural gas light hydrocarbon(C_5-C_7) from the Xujiahe Formation presented two characteristics: the first was the relatively high aromatic hydrocarbon content(19%-32.1%), which reveals the characteristic of natural gas with humic substances of high-maturity; the second was the low content of aromatic hydrocarbon(0.4%-9.3%),reflecting water-washing during the accumulation of the natural gas. The reported research outcomes indicate a potential mechanism for natural gas accumulation in the Xujiahe Formation, which will further guide natural gas exploration in this region. | XIE Zengye LI Jian LI Zhisheng GUO Jianying LI Jin ZHANG Lu DONG Caiyuan | 2017 | Acta Geologica Sinica(English Edition)2017，91，5： | 14 |
| 4 | Quantitative prediction of mining subsidence and its impact on the environment显示文摘This study is focused on the prediction of mining subsidence and its impact on the environment in the Hongqi mining area. The study was carried out by means of a probability integral model based, in first instance based on field surveys and the analysis of data collected from this area. Isolines of mining subsidence were then drawn and the impact caused by mining subsidence on the environment was analyzed quantitatively by spatial analysis with Geographic Information System (GIS). The results indicate that the subsidence area of the first working-mine can be as large as 2.54 km 2 , the maximum subsidence is 3440 mm which will cause 1524 houses to be relocated. The entire subsidence area of the mine can reach 8.09 km 2 , with a maximum subsidence of 3590 mm. Under these circumstances the value of the loss of ecosystem services will reach 5.371 million Yuan and the cost of relocating buildings will increase to 6.858 million Yuan. | Jianjun Song Chunjian Han Ping Li Junwei Zhang Deyuan Liu Minde Jiang Lin Zheng Jingkai Zhang Jianying Song | 2012 | International Journal of Mining Science and Technology2012，22，1： | 13 |
| 5 | Hybrid hierarchy storage system in MilkyWay-2 supercomputer显示文摘 | Weixia XU Yutong LU Qiong LI Enqiang ZHOU Zhenlong SONG Yong DONG Wei ZHANG Dengping WEI Xiaoming ZHANG Haitao CHEN Jianying XING Yuan YUAN | 2014 | Frontiers of Computer Science2014，8，3： | 11 |
| 6 | 考虑喷口烧蚀影响的高压断路器电弧仿真(英文)显示文摘In order to investigate the influence of nozzle ablation to arc,a 2-D axisymmetric magneto-hydro-dynamics arc model is developed to simulate the arc behavior during the whole interruption process in a puffer-type high voltage circuit breaker,considering the nozzle ablating and the mixing process of Polytetrafluoroethylene(PTFE) vapor with SF6.The results show that the arc radius firstly increases and then decreases as arc current varies according to its sinusoida waveform.At 3.5 ms after arc initiation the nozzle ablation starts,and 2.5 ms later the current nearly reaches its peak value and nozzle ablation develops intensely.Meanwhile the nozzle is blocked by arc at this time and consequently the vapor concentration in nozzle reaches the highest.Then while the current decreasing and open distance increaseing the vapor concentration gradually decreases.Before current zero the extinction peak of arc voltage occurs.It indicates that the PTFE vapor enhances pressure rise in arc quenching chamber and strengthens the arc blocking effect on nozzle;the arc radius is wider and arc temperature is lower when nozzle ablation is considered. | JIANG Xu ZHONG Jianying ZHANG Youpeng ZHANG Gaochao LI Xingwen JIA Shenli | 2013 | 高电压技术2013，39，4： | 11 |
| 7 | 热处理前后非脱气交联聚乙烯的介电性能和机械性能分析(英文)显示文摘In order to investigate the influence of cross-linking byproducts on dielectric and mechanical properties of 110k V cross-linked polyethylene(XLPE)cable insulation,we selected the undegassed XLPE cable insulation which is heated at 70℃in laboratory for different duration in terms of heating time,to prepare XLPE samples with different mass loss ratio.We carried out Fourier transform infrared spectroscopy(FTIR)test,electric breakdown test at 50 Hz,pulsed electro-acoustic(PEA)measurement and tensile test for XLPE samples.In FTIR spectrogram,the characteristic absorption peaks were observed to appear at 1 680 cm-1 and 1 600 cm-1,which can be related to two kinds of cross-linking byproducts includingα-methylstyrene and acetophenone.The intensity of these absorption peaks decreases with the increase of mass loss ratio.It can be proposed that the mass loss of XLPE samples in the process of thermal treatment is caused by volatiliztion ofα-methylstyrene and acetophenone.Therefore,the change of mass loss ratio can reflect the change of content ofα-methylstyrene and acetophenone in the process of thermal treatment.The results of electric breakdown test showed that the reduction ofα-methylstyrene and acetophenone leads to the enhancement of electric breakdown strength at 50 Hz.PEA measurement results showed that heterocharge is produced and accumulated when cross-linking byproducts are present,whereas no heterocharge is found whenα-methylstyrene and acetophenone are absent.The formation of heterocharge is attributed to ionization of cross-linking byproducts under electric stress.Based on these results,it can be suggested that when AC electric field is applied on XLPE samples,the occurrence of ionization of cross-linking byproducts results in the increase of free carriers in XLPE insulation,leading to a lower electric breakdown strength.The results of tensile test showed that tensile strength is proportional to the mass loss ratio of XLPE samples,which suggests that cross-linking byproducts can reduce the tensile strength of XLPE insulation. | LI Huan LI Jianying WANG Qimeng ZHOU Yunjie LI Honglei | 2015 | 高电压技术2015，41，4： | 11 |
| 8 | Effects of short-term low temperatures on photosystem Ⅱ function of samara and leaf of Siberian maple (Acer ginnala) and subsequent recovery显示文摘Samara is the reproductive organ (seed) for many tree species in arid land in northwestern China. It is ecologically important in population development due to its dispersal function. However, information on its photosynthesis and effect of environmental stresses on its photosynthesis is still very limited. In the present study, responses of photosystem II (PSII) activity in samara and leaf of Siberian maple to short-term chilling/freezing and subsequent recovery potential were comparatively investigated by using polyphasic fluorescence test. The samara had more efficient photosynthesis (Fv/Fm and PIABS) and more efficient electron transport (φEo) but lower energy dissipation (DIo/RC) than leaf. Generally, the PSII performance and the electron transport for both samara and leaf were inhibited under low temperature stress, accompanied by an increase of energy dissipation in PSII reaction centers (RCs). PSII of both samara and leaf was not markedly affected by chilling and could acclimate to chilling stress. Short-term freezing could completely inhibit PSII activity in both samara and leaf, indicated by the drop of values of Fv/Fm, PIABS, φEo to zero. PSII functional parameters of short-term dark frozen samara could be largely recovered whereas those of frozen leaf could not be recovered. The higher tolerance of samara to short-term low temperature stress than leaf is of great ecological significance for seed development, population establishment of Siberian maple. | XiangLiang PAN1, DaoYong ZHANG1,2, Xi CHEN1, Li LI1, GuiJin MU1, LanHai LI1, AnMing BAO1, Jing LIU2, HuaiSong ZHU1, WenJuan SONG1, JianYing YANG1, JunYong AI1 1 Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China 2 State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550000, China | 2009 | Journal of Arid Land2009，1，1： | 10 |
| 9 | Personalized targeted therapy for esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma continues to heavily burden clinicians worldwide. Researchers have discovered the genomic landscape of esophageal squamous cell carcinoma, which holds promise for an era of personalized oncology care. One of the most pressing problems facing this issue is to improve the understanding of the newly available genomic data, and identify the driver-gene mutations, pathways, and networks. The emergence of a legion of novel targeted agents has generated much hope and hype regarding more potent treatment regimens, but the accuracy of drug selection is still arguable. Other problems, such as cancer heterogeneity, drug resistance, exceptional responders, and side effects, have to be surmounted. Evolving topics in personalized oncology, such as interpretation of genomics data, issues in targeted therapy, research approaches for targeted therapy, and future perspectives, will be discussed in this editorial. | Xiaozheng Kang Keneng Chen Yicheng Li Jianying Li Thomas A D'Amico Xiaoxin Chen | 2015 | World Journal of Gastroenterology2015，21，25： | 10 |
| 10 | Treatment of multiple sclerosis by transplantation of neural stem cells derived from induced pluripotent stem cells显示文摘Multiple sclerosis(MS) is an autoimmune disease of the central nervous system(CNS), with focal T lymphocytic infiltration and damage of myelin and axons. The underlying mechanism of pathogenesis remains unclear and there are currently no effective treatments. The development of neural stem cell(NSC) transplantation provides a promising strategy to treat neurodegenerative disease. However, the limited availability of NSCs prevents their application in neural disease therapy. In this study, we generated NSCs from induced pluripotent stem cells(iPSCs) and transplanted these cells into mice with experimental autoimmune encephalomyelitis(EAE), a model of MS. The results showed that transplantation of iPSC-derived NSCs dramatically reduced T cell infiltration and ameliorated white matter damage in the treated EAE mice. Correspondingly, the disease symptom score was greatly decreased, and motor ability was dramatically rescued in the iPSC-NSC-treated EAE mice, indicating the effectiveness of using iPSC-NSCs to treat MS. Our study provides pre-clinical evidence to support the feasibility of treating MS by transplantation of iPSC-derived NSCs. | Chao Zhang Jiani Cao Xiaoyan Li Haoyu Xu Weixu Wang Libin Wang Xiaoyang Zhao Wei Li Jianwei Jiao Baoyang Hu Qi Zhou Tongbiao Zhao | 2016 | Science China(Life Sciences)2016，59，9： | 9 |
| 11 | Speciation analysis of metals (Tl,Cd and Pb) in Tl-containing pyrite and its cinder from Yunfu Mine,China,by ICP-MS with sequential extraction显示文摘The speciation of heavy metals such as thallium,cadmium and lead existing in pyrite and pyrite cinder was analyzed by ICP-MS with a sequential extraction procedure.The distribution patterns of these metals including exchangeable,reducible,oxidizable and residual fractions were obtained.Tl,Cd and Pb in pyrite and pyrite cinder samples from each extraction step were determined by inductively coupled plasma mass spectrometry(ICP-MS).Under the optimized instrumental conditions,detection limits of Tl,Cd and Pb in different matrices were within the range of 0.006-0.07 μg/L,and the relative standard deviations ranged from 0.8% to 1.2%.The accuracy of Tl,Cd and Pb determination was checked by analyzing two certified reference materials.The results demonstrate that trace Tl,Cd and Pb in the samples can be accurately determined.The sequential extraction results revealed that the percent contents of Tl,Cd and Pb in exchangeable,reducible and oxidizable fractions in pyrite cinder are different from those in pyrite and in pyrite cinder.Tl,Cd and Pb mostly are distributed in residual fraction.Therefore,the mobility of metals in pyrite is higher than that in pyrite cinder.Although distributions of Tl,Cd and Pb in the non-residual fraction are not dominant in pyrite cinder,the total concentrations of them could not be ignored.Consequently,attention must be paid to the risk of potential pollution by pyrite cinder. | WANG Chunlin CHEN Yongheng PAN Jianyang ZHANG Ping QI Jianying LIU Juan LI Xiangping WANG Jin | 2010 | Chinese Journal Of Geochemistry2010，29，1： | 9 |
| 12 | Sintilimab versus docetaxel as second-line treatment in advanced or metastatic squamous non-small-cell lung cancer:an open-label,randomized controlled phase 3 trial(ORIENT-3)显示文摘Background:Treatment options for Chinese patients with locally advanced or metastatic squamous-cell non-small-cell lung cancer(sqNSCLC)after failure of first-line chemotherapy are limited.This study(ORIENT-3)aimed to evaluate the efficacy and safety of sintilimab versus docetaxel as second-line treatment in patients with locally advanced or metastatic sqNSCLC.Methods:ORIENT-3 was an open-label,multicenter,randomized controlled phase 3 trial that recruited patients with stage IIIB/IIIC/IV sqNSCLC after failure with first-line platinum-based chemotherapy.Patients were randomized in a 1:1 ratio to receive either 200 mg of sintilimab or 75 mg/m^(2) of docetaxel intravenously every 3 weeks,stratified by the Eastern Cooperative Oncology Group performance status.The primary endpoint was overall survival(OS)in the full analysis set(FAS).Secondary endpoints included progression-free survival(PFS),objective response rate(ORR),disease control rate(DCR),duration of response(DoR)and safety.Results:Between August 25,2017,and November 7,2018,290 patients were randomized.For FAS,10 patients fromthe docetaxel armwere excluded.Themedian OS was 11.79(n=145;95%confidence interval[CI],10.28-15.57)months with sintilimab versus 8.25(n=135;95%CI,6.47-9.82)months with docetaxel(hazard ratio[HR]:0.74;95%CI,0.56-0.96;P=0.025).Sintilimab treatment significantly prolonged PFS(median 4.30 vs.2.79 months;HR:0.52;95%CI,0.39-0.68;P<0.001)and showed higher ORR(25.50%vs.2.20%,P<0.001)and DCR(65.50%vs.37.80%,P<0.001)than the docetaxel arm.The median DoRwas 12.45(95%CI,4.86-25.33)months in the sintilimab arm and 4.14(95%CI,1.41-7.23)months in the docetaxel arm(P=0.045).Treatment-related adverse events of grade≥3were reported in 26(18.1%)patients in the sintilimab arm and 47(36.2%)patients in the docetaxel arm.Exploratory biomarker analysis showed potential predictive values of expression levels of two transcription factors,including OVOL2(HR:0.35;P<0.001)and CTCF(HR:3.50;P<0.001),for sintilimab treatment.Conclusions:Compared with docetaxel,sintilimab significantly improved the OS,PFS,and ORR of Chinese patients with previously treated locally advanced or metastatic sqNSCLC. | Yuankai Shi Lin Wu Xinmin Yu Puyuan Xing Yan Wang Jianying Zhou Airong Wang Jianhua Shi Yi Hu Ziping Wang Guangyu An Yong Fang Sanyuan Sun Caicun Zhou Changli Wang Feng Ye Xingya Li Junye Wang Mengzhao Wang Yunpeng Liu Yanqiu Zhao Ying Yuan Jifeng Feng Zhendong Chen Jindong Shi Tao Sun Gang Wu Yongqian Shu Qisen Guo Yi Zhang Yong Song Shucai Zhang Yuan Chen Wei Li Hongrui Niu Wenwei Hu Lijun Wang Jianan Huang Yang Zhang Ying Cheng Zhengdong Wu Bo Peng Jiya Sun Christoph Mancao Yanqi Wang Luyao Sun | 2022 | Cancer Communications2022，42，12： | 8 |
| 13 | Simultaneous attack of a stationary target using multiple missiles: a consensus-based approach显示文摘This paper considers the simultaneous attack problem of multiple missiles against a stationary target. Built upon the classic proportional navigation structure, we propose a consensus-based approach to design the cooperative guidance law. Specifically, we present time-varying navigation ratios for the missiles,which exchange the time-to-go estimates between neighboring missiles via a communication network. For the cases where the communication topology is undirected or in the leader–follower structure with a missile acting as the leader whose navigation ratio cannot be tuned, we show that the proposed cooperative guidance law can solve the simultaneous attack problem. The effectiveness of the theoretical results is finally illustrated by numerical simulations. | Jialing ZHOU Jianying YANG Zhongkui LI | 2017 | Science China(Information Sciences)2017，60，7： | 8 |
| 14 | Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys显示文摘 | Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang | 2015 | Cell Research2015，25，2： | 8 |
| 15 | Collinear holographic data storage technologies显示文摘In the era of information explosion,the demand of data storage is increased dramatically.Holographic data storage technology is one of the most promising next-generation data storage technologies due to its high storage density,fast data transfer rate,long data life time and less energy consumption.Collinear holographic data storage technology is the typical solution of the holographic data storage technology which owns a more compact,compatible and practical system.This paper gives a brief review of holographic data storage,introduces collinear holographic data storage technology and discusses phase modulation technology being used in the holographic data storage system to achieve higher storage density and higher data transfer rate. | Xiao Lin Jinpeng Liu Jianying Hao Kun Wang Yuanying Zhang Hui Li Hideyoshi Horimai Xiaodi Tan | 2020 | Opto-Electronic Advances2020，3，3： | 8 |
| 16 | Precise localization of small pulmonary nodules using Pre-VATS with Xper-CT in combination with real-time fluoroscopy-guided coil:report of 15 patients显示文摘Purpose: This study aimed to evaluate the value of precise localization of nodules using pre-video-assisted thoracic surgery(VATS) Xper–CT in combination with real-time fluoroscopy-guided coil in the resection of pulmonary nodules using VATS. Materials and Methods: Precise localization of nodules using Xper-CT in combination with real-time fluoroscopy-guided coil and wedge resection using VATS were conducted on 15 patients with 17 small pulmonary nodules(diameter 0.5–1.5 cm) from April 2015 to January 2016. The value of localization was evaluated in terms of procedure time, type of coils, associated complications of localization, and VATS success rate. Results: The success rate of coil localization was found to be 100% in the primary stage(as shown by the CT scan), and the average procedure time was 30–45 min(35.6 ± 3.05 min). No deaths or major complications occurred. Minor complications included five incidents of pneumothorax(the morbidity was 29.4%, 5/17; no patient required chest tube drainage). The dislocation of coil was found in one patient. The results of pathological examination of 17 small pulmonary nodules revealed 11 primary lung cancers, 1 mesenchymal tumor, 3 nonspecific chronic inflammations, 1 hamartoma, and 1 tuberculosis. Two patients with primary lung cancer underwent lobectomy with mediastinal lymph node dissection. Conclusion: The preoperative precise localization of small pulmonary nodules using Xper-CT-guided coil is an effective and safe technique. It helps in the resection of nodules using VATS. It increases the rate of lung wedge resection with few complications and allows for proper diagnosis with a low thoracotomy conversion rate. | Jiemin Cheng Changyu Li Liangwen Wang Jiting Liang Zhiping Yan Jiani Hu Huibing Shi | 2018 | Journal of Interventional Medicine2018，1，2： | 8 |
| 17 | Cooperative Guidance Law Design for Simultaneous Attack with Multiple Missiles Against a Maneuvering Target显示文摘这份报纸对一个调遣的目标考虑多重导弹的同时的攻击问题。与大多数同时的攻击问题在被提出的存在文学不同当导弹 time-to-go 的一个一致问题估计,这份报纸作为导弹 ranges-to-go 的一致问题提出它。基于这策略,新奇分布式的指导法律被建议与未知可操作性的目标解决同时的攻击问题。适应控制方法被介绍估计目标加速的上面的界限。建议指导法律的有效性理论上并且数字地被验证。 | ZHOU Jialing Lü Yuezu LI Zhongkui YANG Jianying | 2018 | Journal of Systems Science & Complexity2018，31，1： | 8 |
| 18 | Inhibiting autophagy enhances sulforaphane-induced apoptosis via targeting NRF2 in esophageal squamous cell carcinoma显示文摘Sulforaphane(SFN), a natural anti-tumor compound from cruciferous vegetables, has been reported to induce protective autophagy to cancer cells, which might impair the anti-tumor efficiency of SFN. However, the accurate function and mechanism of SFN inducing autophagy in cancers are still obscure, especially in esophageal squamous cell carcinoma(ESCC), one of malignancies with high incidence in North China. Here, we mainly explored the potential function of autophagy upon SFN treatment in ESCC and molecular mechanism. We demonstrated that SFN could inhibit cell proliferation and induce apoptosis by activating caspase pathway. Moreover, we found activation of NRF2 pathway by SFN was responsible for the induction of autophagy and also a disadvantage element to the anti-tumor effects of SFN on ESCC, indicating that SFN might induce protective autophagy in ESCC. We, therefore,investigated effects of autophagy inhibition on sensitivity of ESCC cells to SFN and found that chloroquine(CQ) could neutralize the activation of SFN on NRF2 and enhance the activation of SFN on caspase pathway, thus improved the anti-tumor efficiency of SFN on ESCC in vitro and in vivo. Our study provides a preclinical rationale for development of SFN and its analogs to the future treatment of ESCC. | Zhaoming Lu Yandan Ren Li Yang Ang Jia Yi Hu Yu Zhao Wuduo Zhao Bin Yu Wen Zhao Jianying Zhang Guiqin Hou | 2021 | Acta Pharmaceutica Sinica B2021，11，5： | 8 |
| 19 | Reversal effect of bufalin on multidrug resistance in K562/VCR vincristine-resistant leukemia cell line显示文摘OBJECTIVE: To probe insights into the reversal effect of bufalin on vincristine-acquired multidrug resistance(MDR) in human leukemia cell line K562/VCR.METHODS: Proliferative inhibition rate and the reversal index(RI) of bufalin were determined by Methyl thiazolyl tetrazolium assay. The uptake of Adriamycin(ADM) in K562/VCR cells, cell cycle and apoptosis rate were determined by flow cytometry(FCM). Cell morphologic changes were observed with Wright-Giemsa staining. The expression of P-glycoprotein(P-gp), multidrug-associated protein-1(MRP1), Bcl-x L and Bax protein were measured by immunocytochemistry.RESULTS: The human leukemia multidrug resistant K562/VCR cells showed no cross-resistance to bufalin. The RIs of bufalin at concentrations of 0.0002,0.001 and 0.005 μmol/L were 4.85, 6.94 and 14.77,respectively. Preincubation of 0.001 μmol/L bufalin for 2 h could increase intracellular ADM fluorescence intensity to 28.07%(P<0.05) and down-regulate MRP1 expression simultaneously, but no remarkable effect was found on P-gp protein. Cell cycle analysis indicated increased apoptosis rate and apparent decreased G2/M phase proportion after treatment with bufalin. When exposed to 0.01μmol/L bufalin, typical morphological changes of apoptosis could be observed. Down-regulation of Bcl-x L and up-regulation of Bax expression in K562/VCR cells could be detected by immunocytochemistry.CONCLUSION: Bufalin could partly reverse the MDR of K562/VCR cells, with a possible mechanism of down-regulating MRP1 expression and activating apoptosis pathway by altering Bcl-x L/Bax ratio. | Xiaofeng Zhai Jianying Lu Ying Wang Fanfu Fang Bai Li Wei Gu | 2014 | Journal of Traditional Chinese Medicine2014，34，6： | 7 |
| 20 | Effect of Yiqihuoxue prescription on myocardial energy metabolism after myocardial infarction via cross talk of liver kinase B1-depen-dent Notch1 and adenosine 5'-monophosphate-activated protein kinase显示文摘OBJECTIVE: To investigate the effect of Yiqihuoxue prescription(YQHX) from Traditional Chinese Medicine(TCM) on myocardial glucose and lipid metabolism after myocardial infarction via the cross talk between the liver kinase B1(LKB1)-dependent Notch1 and adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK). YQHX was prepared with substances with properties that benefit, to activate blood circulation based on the TCM theory.METHODS: Animal models of myocardial infarction were established by ligating Sprague Dawley rats' left anterior descending coronary arteries. The animals were randomly divided into a myocardial infarction(MI) group, a YQHX group, a perindopril group, a γ-secretase inhibitor, Notch signal inhibitor(DAPT) group, a DAPT+YQHX group and a sham group. The related drugs were administered on the second day after operation, and changes in the relevant indexes were examined on weeks 1 and 4.Changes in cardiac structure and function were examined by echocardiography. The glucose and free fatty acids(FFA) were examined by ELISA. The expression of Notch, LKB1 and AMPK m RNA was examined by a real-time fluorescence quantitative method. The expression of glucose transporter 4(GLUT4), and the expression of total acetyl-Co A carboxylase(ACC) and its phosphorylation were examined by western blotting.RESULTS: Compared with the sham group, the expression of Notch, LKB1 and AMPK m RNA in the MI group was lower. Compared with the MI group, the expression of these m RNAs in the YQHX and perindopril groups was higher, and their expression in the DAPT group was lower. At all time points, the protein expression of GLUT4 and p ACC decreased in the MI group. On week 1, the expression of p ACC protein was higher. In the DAPT group, the expression of p ACC protein decreased. Compared with the YQHX group, the expression of p ACC protein in the DAPT + YQHX group was lower. On week 4,compared with the MI group, the expression of GLUT4 protein in the YQHX group and the perindo-pril group was higher. The expression of GLUT4 protein in the DAPT group decreased. Compared with the YQHX group, the expression of GLUT4 protein in the DAPT+YQHX group was lower. There was no significant difference in the expression of ACC protein between the groups.CONCLUSION: YQHX promoted cross talk between the LKB1-dependent Notch1 and AMPK in myocardial tissue after myocardial infarction. Furthermore,it regulated the glucose and lipid metabolism of cardiomyocytes at different time points, thereby ameliorating the cardiac energy metabolism via different mechanisms and protecting the heart. | Wu Jiangong Chen Xi Guo Shuwen Liu Wenchen Zhang Lu Li Fanghe Wu Jiani Huang Xiaolou Cai Qian Tan Xiaobo Wang Hui | 2017 | Journal of Traditional Chinese Medicine2017，37，3： | 7 |
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