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| 1 | PI3K/Akt signaling transduction pathway is involved in rat vascular smooth muscle cell proliferation induced by apelin-13显示文摘脉管的光滑的肌肉房间(VSMC ) 被将生物的活组织移植于培养基中培养方法从男 SpragueDawley 老鼠的胸的主动脉准备经由 3 kinase (PI3K )/Akt 发信号 transduction 小径由 apelin-13 导致了的 phosphoinositide 观察 VSMC 增长。PI3K, phospho-PI3K, phospho-Akt, ERK1/2, phospho-ERK1/2 和 cyclin D1 的表示被西方的污点分析检测。结果证明 apelin-13 在剂量依赖者和时间依赖者举止支持了 phospho-PI3K 和 phospho-Akt 的表示。PI3K 禁止者 LY294002 显著地减少了 phospho-PI3K, phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 导致了。Akt 禁止者 1701-1 显著地减少了 phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 刺激了。MTT 试金结果证明 PI3K 禁止者 LY294002 和 Akt 禁止者 1701-1 显著地禁止了 apelin-13 导致的 VSMC 增长。Apelin-13 通过表明 transduction 小径的 PI3K/Akt 支持了 VSMC 增长。 | Changhui Liu Tao Su Fang Li Lanfang Li Xuping Qin Weinan Pan Fen Feng Feng Chen Duanfang Liao Linxi Chen | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 41 |
| 2 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 3 | The localization of type 2 diabetes susceptibility gene loci in northern Chinese Han families显示文摘We conducted a genome-wide scan, in which 358 well distributed fluorescent dye-labeled microsatellite marker sets were applied in 32 Chinese Han type 2 diabetes families from Northern China to search for the susceptibility gene loci. The data collected from screening all the chromosomes of genome were genotyped by using genescan and genotyping software, then, parametric and non-parametric multipoint test, and affected sib-pair analysis as well, were used to analyze the data. We identified some susceptibility gene loci residing in chromosomes 1,12,18,20, respectively, or precisely, located around D1S214, D1S207, D1S218, D1S235, D12S336, D18S61 and D20S118. The comparison of this result with those from other regions and races reflected the complexity and heterogeneity of type 2 diabetes. | ZHAO Jinying WANG Heng XIONG Momiao HUANG Wei ZUO Jin CHEN Zhu QIANG Boqin SUN Qi LI Yuxiu LIU Qiuying DU Weinan CHEN Jialun DING Wei YUAN Wentao ZHAO Yang XU Hongyan JIN Li FANG Fude | 2000 | Chinese Science Bulletin2000，45，19： | 8 |
| 4 | Highly luminescent and stable CsPbBr3 perovskite quantum dots modified by phosphine ligands显示文摘All-inorganic cesium lead halide perovskite quantum dots (QDs) have been a promising candidate for optoelectronic devices in recent years,such as light-emitting diodes,photodetectors and solar cells,owing to their superb optoelectronic properties.Still,the stability issue of nanocrystals is a bottleneck for their practical application.Herein,we report a facile method for the synthesis of a series of phosphine ligand modified CsPbBr3 QDs with high PL intensity.By introducing organic phosphine ligands,the tolerance of CsPbBr3 QDs to ethanol,water and UV light was dramatically improved.Moreover,the phosphine ligand modified QD films deposited on the glass subtracts exhibit superior PL intensity and optical stability to those of pristine QD based films. | Yan Li Xiaoyan Wang Weinan Xue Wei Wang Wei Zhu Lianjing Zhao | 2019 | Nano Research2019，12，4： | 7 |
| 5 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 6 | Signal pathways of melanoma and targeted therapy显示文摘Melanoma is the most lethal skin cancer that originates from the malignant transformation of melanocytes.Although melanoma has long bee n regarded as a cancerous malig nancy with few therapeutic opti ons,in creased biological understandi ng and unprecedented innovations in therapies targeting mutated driver genes and immune checkpoints have substantially improved the prog nosis of patients.However,the low response rate and inevitable occurre nee of resistance to curre ntly available targeted therapies have posed the obstacle in the path of melanoma management to obtain further amelioration.Therefore,it is necessary to understand the mechanisms underlying melanoma pathogenesis more comprehensively,which might lead to more substantial progress in therapeutic approaches and expand clinical options for melanoma therapy.In this review,we firstly make a brief introduction to melanoma epidemiology,clinical subtypes,risk factors,and current therapies.Then,the signal pathways orchestrati ng mela noma pathogenesis,in eluding gen etic mutatio ns,key tran scripti onal regulators,epige netic dysregulati ons,metabolic reprogramming,crucial metastasis-related signals,tumor-promoting inflammatory pathways,and pro-angiogenic factors,have been systemically reviewed and discussed.Subsequently,we outline current progresses in therapies targeting mutated driver genes and immune checkpoi nts,as well as the mecha nisms underlying the treatment resista nee.Finally,the prospects and challe nges in the developme nt of mela noma therapy,especially immu no therapy and related ongoing din ical trials,are summarized and discussed. | Weinan Guo Huina Wan Chunying Li | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 4 |
| 7 | High thermal stability Cu_(2)O@OZrP micro-nano hybrids for melt-spun excellent antibacterial activity polyester fibers显示文摘Public safety incidents caused by bacterial infections have attracted widespread attention towards antibacterial textiles(fibers,fabrics,etc.).Nevertheless,it is still challenging to efficiently load inorganic nano-antibacterial materials in polymer fibers.In this work,zirconium phosphate(ZrP,layered micro-nano materials)was utilized as a micro-nano carrier.The octadecyl triphenyl phosphonium bromide(OTP)was intercalated between the ZrP sheets by the ion exchange method to improve the carrier-polymer compatibility and the antibacterial performance.Through in-situ chemical reduction,the ultra-small nano-sized cuprous oxide(Cu_(2)O<5 nm)was loaded on the outer surface of ZrP to realize the uniform and stable dispersion of the Cu_(2)O on the carrier and improve the antibacterial performance.The ZrP nanosheets loaded with Cu_(2)O and OTP(Cu_(2)O@OZrP)had excellent antibacterial properties,and the antibacterial rate against E.coli,S.aureus and C.albicans was more than 99%.The intercalation amount of OTP in Cu_(2)O@OZrP can reach 16%,and the thermal stability was excellent and a significant increase in the Zeta potential.Indeed,the decomposition temperature was greater than 350℃,which was suitable for high-temperature melt processing of polymers.Consequently,we prepared PET/Cu_(2)O@OZrP fibers using polyethylene glycol terephthalate(PET),which accounts for 70%of the total chemical fibers,as the fiber matrix.PET/Cu_(2)O@OZrP fibers exhibited excellent mechanical property and antibacterial performance when the content of Cu_(2)O@OZrP was only 0.2%.The antibacterial rate against five types of bacteria including super bacteria(MRSA,VRE)was more than 99%. | Jialiang Zhou Yaping Wang Weinan Pan Hengxue Xiang Peng Li Zhe Zhou Meifang Zhu | 2021 | Journal of Materials Science & Technology2021，，22： | 4 |
| 8 | Heterogeneous Multiscale Methods: A Review显示文摘This paper gives a systematic introduction to HMM,the heterogeneous multiscale methods,including the fundamental design principles behind the HMM philosophy and the main obstacles that have to be overcome when using HMM for a particular problem.This is illustrated by examples from several application areas,including complex fluids,micro-fluidics,solids,interface problems,stochastic problems,and statistically self-similar problems.Emphasis is given to the technical tools,such as the various constrained molecular dynamics,that have been developed,in order to apply HMM to these problems.Examples of mathematical results on the error analysis of HMM are presented.The review ends with a discussion on some of the problems that have to be solved in order to make HMM a more powerful tool. | Weinan E Bjorn Engquist Xiantao Li Weiqing Ren Eric Vanden-Eijnden | 2007 | Communications in Computational Physics2007，2，3： | 4 |
| 9 | Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var.Hangzhouwanensis显示文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity. | Yan Li Lingxin Kong Jufang Shen Qing Wang Qian Liu Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 4 |
| 10 | Different responses of soil respiration and its components to nitrogen and phosphorus addition in a subtropical secondary forest显示文摘Background:Nitrogen(N)and phosphorus(P)deposition have largely affected soil respiration(Rs)in forest ecosystems.However,few studies have explored how N and P individually or in combination to influence Rs and its components(autotrophic respiration,Ra;heterotrophic respiration,Rh),especially in highly P-limited subtropical forests.To address this question,we conducted a field manipulation experiment with N and/or P addition in a 50-year-old subtropical secondary forest.Results:We found that N addition on average reduced Rs,Ra,and Rh by 15.2%,15%,and 11.7%,respectively during 2-year field study.P addition had an inconsistent effect on Ra,with Ra increasing by 50.5%in the first year but reducing by 26.6%in the second year.Moreover,P addition on average decreased Rh by 8.9%–30.9%and Rs by 6.7%–15.6%across 2 years.In contrast,N and P co-addition on average increased Rs,Ra,and Rh by 1.9%,7.9%,and 2.1%during the experimental period.Though Rs and Rh were significantly correlated with soil temperature,their temperature sensitivities were not significantly changed by fertilization.Ra was predominantly regulated by soil nitrogen availability(NH4+and NO3−),soil dissolved organic carbon(DOC),and enzyme activities,while the variation in Rh was mainly attributable to changes in soil microbial community composition and soilβ-D-Cellubiosidase(CB)andβ-Xylosidase(XYL)activities.Conclusion:Our findings highlight the contrasting responses of Rs and its components to N or P addition against N and P co-addition,which should be differentially considered in biogeochemical models in order to improve prediction of forest carbon dynamics in the context of N and P enrichment in terrestrial ecosystems. | Junjun Zhang Yong Li Jinsong Wang Weinan Chen Dashuan Tian Shuli Niu | 2021 | Forest Ecosystems2021，8，3： | 3 |
| 11 | CypA Regulates AIP4-Mediated M1 Ubiquitination of Influenza A Virus显示文摘Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV. | Madina Mahesutihan Weinan Zheng Liang Cui Yun Li Pengtao Jiao Wenxian Yang Wei Liu Jing Li Wenhui Fan Limin Yang Wenjun Liu Lei Sun | 2018 | Virologica Sinica2018，33，5： | 3 |
| 12 | Injectable silk/hydroxyapatite nanocomposite hydrogels with vascularization capacity for bone regeneration显示文摘Localized and sustained osteogenic-angiogenic stimulation to bone defects is critical for effective bone repair.Here,desferrioxamine(DFO)was loaded on silk fibroin nanofibers and blended with hydroxyapatite nanorods(HA),forming injectable DFO-loaded silk fibroin-HA nanocomposite hydrogels.The composite hydrogels remained homogeneous distribution of HA with high ratio(60%)and also higher stiffness than that of pure silk fibroin nanofiber hydrogels,which provided stable osteogenic stimulation niches for tissue regeneration.Without the scarify of injectability,the hydrogels achieved slow delivery of DFO for above 60 days,resulting in suitable angiogenesis in vitro and in vivo and better osteogenesis than DFO-free systems.Compared to previous injectable silk fibroin-HA hydrogels,the introduction of vascularization capacity further stimulated the osteogenic differentiation of stem cells and accelerated new bone formation.Quicker and better bone healing were detected at defect sites after the injection of DFO-loaded nanocomposite hydrogels,indicating the effective synergistic effect of osteogenic and angiogenic cues.This work provides a simple and effective strategy of introducing angiogenic cues to bone matrices.We believe that the injectable nanocomposite hydrogels are suitable for the regeneration of bone tissues. | Keke Wang Weinan Cheng Zhaozhao Ding Gang Xu Xin Zheng Meirong Li Guozhong Lu Qiang Lu | 2021 | Journal of Materials Science & Technology2021，，4： | 2 |
| 13 | Tanshinone IIa antagonizes spinal ischemia/reperfusion injury by interfering with upstream glutamic acid factors显示文摘Based on the hypothesis that upstream factor inhibition results in better treatment effects than downstream factor inhibition, the present study interfered with glutamic acid (Glu)-released upstream factors, such as Glu transporter function and Na+-K+-adenosine triphosphatases (ATPase) activity relativly. Rats with spinal cord ischemia/reperfusion injury received intraperitoneal injections of tanshinone IIa and Glu uptake and Na+-K+-ATPase activity were increased. Results showed that tanshinone IIa influenced Glu-released upstream factors following spinal ischemia/reperfusion injury and protected against spinal ischemia/reperfusion injury. | Li Zhang Xiang Lin Weinan Liu Jianhua Lin | 2010 | Neural Regeneration Research2010，5，20： | 2 |
| 14 | A fast recursive algorithm for two-dimensional thresholding 显示文摘 | Gong Jian Li Liyuan Chen Weinan | 1996 | Signal Processing1996，2，： | 1 |
| 15 | External field assisted hydrogen evolution reaction显示文摘As a clean,efficient,and sustainable energy,hydrogen is expected to replace traditional fossil energy.A series of studies focusing on morphology regulation,surface modification,and structural reconstruction have been devoted to improving the intrinsic catalytic activity of non-noble metal catalysts.However,complex system structure design and the mutual interference of various chemical components would hinder the further improvement of hydrogen evolution performance.In recent years,external field assisted hydrogen evolution reaction(HER)has become a new research hotspot.Herein,we systematically summarize the promoting effects of various external fields on catalytic hydrogen production from the aspects of system design and catalytic mechanism,including electric field,thermal field,optical field,magnetic field,and acoustic field.Ultimately,we discuss the key challenges facing this external field regulation strategy and put forward the prospect of future research topics.We sincerely expect that this review could not only provide a new insight into the basic mechanism of external-assisted catalysis,but also promote further research on improving HER performance from a more diverse and comprehensive perspective. | Jingwen Li Weinan Yin Junan Pan Yingbo Zhang Fengshun Wang Longlu Wang Qiang Zhao | 2023 | Nano Research2023，16，7： | 1 |
| 16 | Fast recursive algorithm for two-dimensional thresholding显示文摘 | Gong Jian Li Liyuan Chen Weinan | 1998 | Pattern Recognition1998，31，3： | 1 |
| 17 | Effects of amendment of biochar-manure compost in conjunction with pyroligneous solution on soil quality and wheat yield of a salt-stressed cropland from Central China Great Plain显示文摘 | Muhammad Siddique Lashari Yuming Liu Lianqing Li Weinan Pan Jiaying Fu Genxing Pan Jufeng Zheng Jinwei Zheng Xuhui Zhang Xinyan Yu | 2012 | Field Crops Research2012，，： | 1 |
| 18 | Fast recursive algorithm for two-dimensional thresholding显示文摘 | GONG Jian LI liyuan CHEN Weinan | 1998 | Pattern Recognition1998，31，3： | 1 |
| 19 | Transdermal enhancement effect and mechanism of iontophoresis for non-steroidal anti-inflammatory drugs显示文摘 | Zuo Jing Du Lina Li Miao Liu Boming Zhu Weinan Jin Yiguang | 2014 | 2014 (1-2)2014，，1： | 1 |
| 20 | Corner detection and interpretation on planar curves using fuzzy reasoning显示文摘 | Li Liyuan Chen Weinan | 1999 | IEEE Transactions on Pattern Analysis and Machine Intelligence1999，21，11： | 1 |
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