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| 1 | Individualized prediction of perineural invasion in colorectal cancer: development and validation of a radiomics prediction model显示文摘Objective: To develop and validate a radiomics prediction model for individualized prediction of perineural invasion(PNI) in colorectal cancer(CRC).Methods: After computed tomography(CT) radiomics features extraction, a radiomics signature was constructed in derivation cohort(346 CRC patients). A prediction model was developed to integrate the radiomics signature and clinical candidate predictors [age, sex, tumor location, and carcinoembryonic antigen(CEA) level]. Apparent prediction performance was assessed. After internal validation, independent temporal validation(separate from the cohort used to build the model) was then conducted in 217 CRC patients. The final model was converted to an easy-to-use nomogram.Results: The developed radiomics nomogram that integrated the radiomics signature and CEA level showed good calibration and discrimination performance [Harrell's concordance index(c-index): 0.817; 95% confidence interval(95% CI): 0.811–0.823]. Application of the nomogram in validation cohort gave a comparable calibration and discrimination(c-index: 0.803; 95% CI: 0.794–0.812).Conclusions: Integrating the radiomics signature and CEA level into a radiomics prediction model enables easy and effective risk assessment of PNI in CRC. This stratification of patients according to their PNI status may provide a basis for individualized auxiliary treatment. | Yanqi Huang Lan He Di Dong Caiyun Yang Cuishan Liang Xin Chen Zelan Ma Xiaomei Huang Su Yao Changhong Liang Jie Tian Zaiyi Liu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 23 |
| 2 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 3 | Migration of Depocenters and Accumulation Centers and its Indication of Subsidence Centers in the Mesozoic Ordos Basin显示文摘与岩性学和岩相在联合基于结构归属的综合学习和原来的盆的特征,中生代 Ordos 盆的沉积起源分析和厚度分发, depocenters 从东南逆时针方向移居了到北方然后到西南从,这被表明中间迟了对早白垩纪三叠纪。有不统一了并且除了在早白垩纪前的几个小孤立的累积中心的大规模累积中心。相对厚的阶层的带很好在几个阶段在西方的盆被开发的原因是这个区域在原来的 Ordos 盆的西方边界附近,有丰富的沉积供应,水动力学效果是强壮的。因此,他们代表本地累积中心。直到早白垩纪, depocenters,累积中心和沉淀,中心在 Ordos 盆的西南部分作为一个实体被迭加。直到中间的侏罗记的结束,仍然在那里出现了在到黄河的西方的剩余盆的一个 paleogeographic 和 paleostructural higher-in-west 和 lower-in-east 框架。从对中间的侏罗记晚三叠纪的中间的 Ordos 盆的 depocenters 一致地被迭加。在 depocenters 和他们的邻居的中生代和古生代的阶层的相对高的热成熟在这些区域建议活跃深效果。通常,在有高热的进化区域的几个时期和他们的一致性的 depocenters 的重叠揭示沉淀进程的控制。因此, depocenters 可以代表沉淀中心的位置。沉淀中心(或 depocenters ) 位于大规模 cratonic Ordos 盆的南方。这与低岬的曲折沉淀被联系,源于强壮的集中和与 Qinling 造山运动同时期的 orogenic 活动。 | ZHAO Junfeng LIU Chiyang WANG Xiaomei ZHANG Can | 2009 | Acta Geologica Sinica(English Edition)2009，83，2： | 12 |
| 4 | Aluminium is essential for root growth and development of tea plants(Camellia sinensis)^∞显示文摘On acid soils,the trivalent aluminium ion(Al3+)predominates and is very rhizotoxic to most plant species.For some native plant species adapted to acid soils including tea(Camellia sinensis),Al3+has been regarded as a beneficial mineral element.In this study,we discovered that Al3+is actually essential for tea root growth and development in all the tested varieties.Aluminum ion promoted new root growth in five representative tea varieties with dose-dependent responses to Al3+availability.In the absence of Al3+,the tea plants failed to generate new roots,and the root tips were damaged within 1 d of Al deprivation.Struc-tural analysis of root tips demonstrated that Al was required for root meristem development and activity.In situ morin@staining of Al3+in roots revealed that Al mainly localized to nuclei in root meristem cells,but then gradually moved to the cytosol when Al3+was subsequently withdrawn.This movement of Al3+from nuclei to cytosols was accompanied by exacerbated DNA damage,which suggests that the nuclear-targeted Al primarily acts to maintain DNA integrity.Taken together,these results provide novel evidence that Al3+is essential for root growth in tea plants through maintenance of DNA integrity in meristematic cells. | Lili Sun Mengshi Zhang Xiaomei Liu Qianzhuo Mao Chen Shi Leon VKochian Hong Liao | 2020 | Journal of Integrative Plant Biology2020，62，7： | 12 |
| 5 | The development of MOFs-based nanomaterials in heterogeneous organocatalysis显示文摘Metal-organic framework(MOF) is a class of inorganic-organic hybrid material assembled periodically with metal ions and organic ligands. MOFs have always been the focuses in a variety of frontier fields owing to the advantageous properties, such as large BET surface areas, tunable porosity and easyfunctionalized surface structure. Among the various application areas, catalysis is one of the earliest application fields of MOFs-based materials and is one of the fastest-growing topics. In this review, the main roles of MOFs in heterogeneous organocatalysis have been systematically summarized, including used as support materials(or hosts), independent catalysts, and sacrificial templates. Moreover, the application prospects of MOFs in photocatalysis and electrocatalysis frontiers were also mentioned.Finally, the key issues that should be conquered in future were briefly sketched in the final parts of each item. We hope our perspectives could be beneficial for the readers to better understand these topics and issues, and could also provide a direction for the future exploration of some novel types of MOFs-based nanocatalysts with stable structures and functions for heterogeneous catalysis. | Xiaomei Liu Bing Tang Jilan Long Wei Zhang Xiaohong Liu Zakaria Mirza | 2018 | Science Bulletin2018，63，8： | 10 |
| 6 | Vertical response of microbial community and degrading genes to petroleum hydrocarbon contamination in saline alkaline soil显示文摘A column microcosm was conducted by amending crude oil into Dagang Oilfield soil to simulate the bioremediation process. The dynamic change of microbial communities and metabolic genes in vertical depth soil from 0 to 80 cm were characterized to evaluate the petroleum degradation potential of indigenous microorganism. The influence of environmental variables on the microbial responds to petroleum contamination were analyzed. Degradation extent of 42.45% of n-alkanes(C8–C40) and 34.61% of 16ΣPAH were reached after 22 weeks. Relative abundance of alkB, nah, and phe gene showed about 10-fold increment in different depth of soil layers. Result of HTS profiles demonstrated that Pseudomonas, Marinobacter and Lactococcus were the major petroleum-degrading bacteria in0–30 and 30–60 cm depth of soils. Fusarium and Aspergillus were the dominant oil-degrading fungi in the 0–60 cm depth of soils. In 60–80 cm deep soil, anaerobic bacteria such as Bacteroidetes, Lactococcus, and Alcanivorax played important roles in petroleum degradation.Redundancy analysis(RDA) and correlation analysis demonstrated that petroleum hydrocarbons(PHs) as well as soil salinity, clay content, and anaerobic conditions were the dominant effect factors on microbial community compositions in 0–30, 30–60, and 60–80 cm depth of soils, respectively. | Qinglong Liu Jingchun Tang Xiaomei Liu Benru Song Meinan Zhen Nicholas J.Ashbolt | 2019 | Journal of Environmental Sciences2019，31，7： | 9 |
| 7 | Modified Mechanism of Eutectic silicon in Al_2O_3/Al-Si Alloy显示文摘Al2O3/Al-Si alloy composite was manufactured by squeeze casting. The morphology of the eutectic silicon in the composite was observed by scanning electronic microscope (SEM), and the modified mechanism of eutectic silicon in the composite was approached. The alumina fiber in the composite can trigger twin during the growth of Al-Si eutectic and lead to the modification of eutectic silicon near the fiber. | Zheng Liu, Xiaomei Liu, Yinglu Zhu, Cigao Chen Southern Institute of Metallurgy, Ganzhou 341000, China | 2001 | Rare Metals2001，20，3： | 9 |
| 8 | Mild moxibustion at Tianshu (ST 25) decreases expression of prokineticin-1 and prokineticin receptor-1 in colon tissue of rats with chronic visceral hyperalgesia显示文摘Prokineticin-1 and prokineticin receptor-1 play important roles in visceral hypersensitivity and in-flammatory pain. Visceral hypersensitivity is closely associated with irritable bowel syndrome. Mild moxibustion can relieve chronic visceral hyperalgesia in rats with irritable bowel syndrome. We hypothesized that prokineticin-1 and prokineticin receptor-1 is the key target in the mechanism. This study established chronic visceral hyperalgesia rat models by colorectal distention. Protein and mRNA expression of prokineticin-1 and prokineticin receptor-1 were determined by immunohisto-chemical method and fluorescence quantitative-PCR, respectively, and were found to be signifi-cantly increased in visceral hyperalgesic rats. Mild moxibustion at Tianshu (ST 25) decreased prokineticin-1 and prokineticin receptor-1 expression in chronic visceral hyperalgesia rats and lessen the chronic visceral hyperalgesia in rats with irritable bowel syndrome at different levels of colorectal distention pressure. | Luyi Wu Chunhui Bao Linbao Ge Cili Zhou Huirong Liu Li Qi Tao Yi Huangan Wu Xiaomei Wang | 2011 | Neural Regeneration Research2011，6，33： | 9 |
| 9 | Analgesic action of suspended moxibustion in rats with chronic visceral hyperalgesia correlates with enkephalins in the spinal cord显示文摘Rats that modeled chronic visceral hyperalgesia received suspended moxibustion at bilateral Tianshu (ST25) and Shangjuxu (ST37) once daily over a period of 7 days. Results show that suspended moxibustion significantly depressed abdominal withdrawal reflex scores and increased enkephalin concentration in the spinal cord. The experimental findings suggest that spinal enkephalins contributed to the analgesic effect of suspended moxibustion in rats with chronic visceral hyperalgesia. | Tao Yi Li Qi Huangan Wu Xiaopeng Ma Huirong Liu Xiaomei Wang | 2012 | Neural Regeneration Research2012，7，3： | 9 |
| 10 | Generation of ready to use (RTU) products over China based on Landsat series data显示文摘Earth observation community has entered into the era of big data.Family of Landsat sensors have collected massive medium resolution satellite images,which are valuable for long-term land surface monitoring.In order to significantly reduce the magnitude of data processing for remote sensing data users,Landsat-based Ready to Use(RTU)products have been produced.Main RTU products,including orthorectified products,land surface reflectance,land surface temperature,large-area mosaic image,and standard image map products,are described.The resulting Landsat RTU products are hosted on the RSGS earth observation data sharing web site for free download(http://gffzz959b1a8e74bf485dhwvqbvq9wp5wv69pp.ffgz.tsg.suse.edu.cn/rtu/).These new products will provide consistent,standardized,multi-decadal image data for robust land cover change detection and monitoring across the Earth sciences.In the coming years,CASEarth DataBank system will be constructed,which is an intelligent data service platform for providing not only the RTU products from multi-source satellite data,but also big earth data analysis methods. | Guojin He Zhaoming Zhang Weili Jiao Tengfei Long Yan Peng Guizhou Wang Ranyu Yin Wei Wang Xiaomei Zhang Huichan Liu Bo Cheng Bo Xiang | 2018 | Big Earth Data2018，2，1： | 8 |
| 11 | Overexpression of C-terminal fragment of glutamate receptor 6 prevents neuronal injury in kainate-induced seizure via disassembly of Glu R6-PSD95-MLK3 signaling module显示文摘Our previous study showed that when glutamate receptor(Glu R)6 C terminus-containing peptide conjugated with the human immunodeficiency virus Tat protein(Glu R6)-9c is delivered into hippocampal neurons in a brain ischemic model, the activation of mixed lineage kinase 3(MLK3) and c-Jun NH2-terminal kinase(JNK) is inhibited via Glu R6-postsynaptic density protein 95(PSD95). In the present study, we investigated whether the recombinant adenovirus(Ad) carrying Glu R6 c could suppress the assembly of the Glu R6-PSD95-MLK3 signaling module and decrease neuronal cell death induced by kainate in hippocampal CA1 subregion. A seizure model in Sprague-Dawley rats was induced by intraperitoneal injections of kainate. The effect of AdGlur6-9c on the phosphorylation of JNK, MLK3 and mitogen-activated kinase kinase 7(MKK7) was observed with western immunoblots and immunohistochemistry. Our findings revealed that overexpression of Glu R6 c inhibited the interaction of Glu R6 with PSD95 and prevented the kainate-induced activation of JNK, MLK3 and MKK7. Furthermore, kainate-mediated neuronal cell death was significantly suppressed by Glu R6 c. Taken together, Glu R6 may play a pivotal role in neuronal cell death. | Jie Mou Xiaomei Liu Dongsheng Pei | 2014 | Neural Regeneration Research2014，9，23： | 6 |
| 12 | Electroacupuncture at Tianshu (ST 25) for diarrhea-predominant irritable bowel syndrome using positron emission tomography Changes in visceral sensation center显示文摘Previous studies have demonstrated that electroacupuncture therapy is effective in the treatment of irritable bowel syndrome. However, the precise mechanism of this therapy is unknown. The present study served to investigate the effects of electroacupuncture therapy on treatment of patients with diarrhea-predominant irritable bowel syndrome (IBS). We compared brain activation maps based on the changes of cerebral glucose metabolism obtained by 18-fluorodeoxyglucose positron emission tomography scanning under three conditions: resting, rectal balloon distension and rectal balloon distension plus electroacupuncture. Under the resting condition, compared with healthy controls, IBS patients displayed an increasing regional cerebral metabolic rate of glucose over a wide range: bilateral superior temporal gyrus, right middle occipital gyrus, superior frontal gyrus and bilateral middle frontal gyrus. However, there was no significant activity in the visceral pain center. Compared with the resting condition, under the rectal balloon distension condition, patients with IBS had a greater regional cerebral metabolic rate of glucose in the prefrontal cortex, left anterior cingulate cortex, postcentral gyrus, precentral gyrus and temporal gyrus. Under the rectal balloon distension plus electroacupuncture condition, stimulation by electroacupuncture at Tianshu (ST 25) manifested a decreased regional cerebral metabolic rate of glucose in the left cingulate gyrus, right insula, right caudate nucleus, fusiform gyrus and hippocampal gyrus. Electroacupuncture therapy relieved abdominal pain, distension or discomfort by decreasing glucose metabolism in the brain. | Huirong Liu Li Qi Xiaolong Wang Yihui Guan Chuantao Zuo Linying Tan Lingsong Yuan Xiaopeng Ma Xiaomei Wang Enhua Zhou Huangan Wu | 2010 | Neural Regeneration Research2010，5，16： | 6 |
| 13 | Progressive loss of striatal dopamine terminals in MPTP-induced acute parkinsonism in cynomolgus monkeys using vesicular monoamine transporter type 2 PET imaging([^(18)F]AV-133)显示文摘The 1-methyl-4-phenyl-1,2,3,4-tetrahydropyridine(MPTP)-induced parkinsonism model, particularly in non-human primates, remains the gold-standard for studying the pathogenesis and assessing novel therapies for Parkinson's disease. However, whether the loss of dopaminergic neurons in this model is progressive remains controversial, mostly due to the lack of objective in vivo assessment of changes in the integrity of these neurons. In the present study, parkinsonism was induced in cynomolgus monkeys by intravenous administration of MPTP(0.2 mg/kg) for 15 days; stable parkinsonism developed over 90 days, when the symptoms were stable. Noninvasive positron emission tomographic neuroimaging of vesicular monoamine transporter 2 with 9-[18F]fluoropropyl-(+)-dihydrotetrabenazine([18F]AV-133) was used before, and 15 and 90 days after the beginning of acute MPTP treatment. The imaging showed evident progressive loss of striatal uptake of [18F]AV-133. The dopaminergic denervation severity had a significant linear correlation with the clinical rating scores and the bradykinesia subscores. Thesefindings demonstrated that [18F]AV-133 PET imaging is a useful tool to noninvasively evaluate the evolution of monoaminergic terminal loss in a monkey model of MPTP-induced parkinsonism. | Yajing Liu Feng Yue Rongping Tang Guoxian Tao Xiaomei Pan Lin Zhu Hank F.Kung Piu Chan | 2014 | Neuroscience Bulletin2014，30，3： | 6 |
| 14 | Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma显示文摘Background:We previously showed that the expression of follistatin-like protein 1(FSTL1)was significantly down-regulated in metastatic clear-cell renal cell carcinoma(ccRCC).In this study,we aimed to characterize the role of FSTL1 in the development of ccRCC.Methods:The effects of FSTL1 on cell activity and cell cycle were investigated in ccRCC cell lines with altered FSTL1 expression.Gene expression microarray assays were performed to identify the major signaling pathways affected by FSTL1 knockdown.The expression of FSTL1 in ccRCC and its effect on postoperative prognosis were estimated in a cohort with 89 patients.Results:FSTL1 knockdown promoted anchorage-independent growth,migration,invasion,and cell cycle of ccRCC cell lines,whereas FSTL1 overexpression attenuated cell migration.FSTL1 knockdown up-regulated nuclear factor-κB(NF-κB)and hypoxia-inducible factor(HIF)signaling pathways,increased epithelial-to-mesenchymal transition,up-regulated interleukin-6 expression,and promoted tumor necrosis factor-α-induced degradation of NF-κB inhibitor(IκBα)in ccRCC cell lines.FSTL1 immunostaining was selectively positive in epithelial cytoplasm in the loop of Henle,and positive rate of FSTL1 was significantly lower in ccRCC tissues than in adjacent renal tissues(P<0.001).The mul-tivariate Cox regression analysis showed that the intratumoral FSTL1 expression conferred a favorable independent prognosis with a hazard ratio of 0.325(95%confidence interval 0.118-0.894).HIF-2αexpression was negatively cor-related with FSTL1 expression in ccRCC specimens(r=−0.229,P=0.044).Intratumoral expression of HIF-2α,rather than HIF-1α,significantly predicted an unfavorable prognosis in ccRCC(log-rank,P=0.038).Conclusions:FSTL1 plays a tumor suppression role possibly via repressing the NF-κB and HIF-2αsignaling pathways.To increase FSTL1 expression might be a candidate therapeutic strategy for metastatic ccRCC. | Yan Liu Xiaojie Tan Wenbin Liu Xi Chen Xiaomei Hou Dan Shen Yibo Ding Jianhua Yin Ling Wang Hongwei Zhang Yongwei Yu Jianguo Hou Timothy C.Thompson Guangwen Cao | 2018 | Cancer Communications2018，38，1： | 5 |
| 15 | Proto-Tethys ophiolitic mélange in SW Yunnan: Constraints from zircon U-Pb geochronology and geochemistry显示文摘An early Paleozoic Proto-Tethys ocean in western Yunnan has long been postulated although no robust geological evidence has been identified.Here we investigated the recently-identified Mayidui and Wanhe ophiolitic mélanges in SW Yunnan,which occurs in a N-S trending belt east of the late Paleozoic Changning-Menglian suture zone.The ophiolites consist mainly of meta-basalts(amphibole schists),meta-(cumulate)gabbros and gabbroic diorites,and meta-chert-shale,representing ancient oceanic crust and pelagic and hemipelagic sediments,respectively.Six samples of gabbros and gabbroic diorites from 3 profiles(Mayidui,Kongjiao and Yinchanghe)yielded zircon U-Pb ages between 462±6 Ma and 447±9 Ma,constraining the formation of the Mayidui and Wanhe ophiolites to Middle Ordovician.Gabbros from the Mayidui and Kongjiao profiles share similar geochemical characteristics with affinities to tholeiitic series,and are characterized by depleted to slightly enriched LREEs relative to HREEs with(La/Sm)N=0.69-1.87,(La/Yb)N=0.66-4.72.These,along with their predominantly positive wholerock eNd(t)and zircon eHf(t)values,indicate a MORB-like magma source.By contrast,the meta-mafic rocks from the Yinchanghe profile show significantly enriched LREEs((La/Sm)N=0.97-3.33,(La/Yb)N=1.19-14.93),as well as positive whole-rock eNd(t)and positive to negative zircon eHf(t)values,indicating an E-MORB-type mantle source.These geochemical features are consistent with an intra-oceanic setting for the formation of the Mayidui-Wanhe ophiolites.Our data,integrated with available geological evidence,provide robust constraints on the timing and nature of the Mayidui-Wanhe ophiolitic mélange,and suggest that the ophiolites represent remnants of the Proto-Tethys Ocean,which opened through separation of the Indochina and Simao blocks from the northern margin of Gondwana before the Early Cambrian,and evolved through to the Silurian. | Guichun Liu Zaibo Sun Jianwei Zi M.Santosh Tianyu Zhao Qinglai Feng Guangyan Chen Xiaomei Nie Jing Li Shitao Zhang | 2021 | Geoscience Frontiers2021，12，5： | 5 |
| 16 | Refining mechanism of yttrium on primary α-Al in semi-solid Al alloy显示文摘Semi-solid Al7Si0.3Mg alloy slurry, which is grain-refined by yttrium, is manufactured by low temperature pouring. The effects of grain-refined by yttrium on the morphology and the grain size of the primary α-Al in semi-solid Al7Si0.3Mg alloy are researched. The results indicate that semi-solid Al7Si0.3Mg alloy with particle-like and rosette-like primary α-Al can be prepared by low temperature pouring from liquid Al7Si0.3Mg alloy grain-refined by yttrium. The grain size and particle morphology of primary α-Al in Al7Si0.3Mg alloy are markedly improved by 0.5 wt pct Y. The refining mechanism of Y on the morphology and the grain size of the primary α-Al in semi-solid Al7Si0.3Mg alloy are delved. | Zheng LIU Yongmei HU Xiaomei LIU | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，4： | 5 |
| 17 | IL-17-triggered downregulation of miR-497 results in high HIF-1α expression and consequent IL-1β and IL-6 production by astrocytes in EAE mice显示文摘Interleukin 17(IL-17)is increasingly recognized as a key factor that contributes to the pathogenesis of multiple sclerosis(MS)and its experimental mouse autoimmune encephalomyelitis(EAE)model.However,the roles and regulatory mechanisms of IL-17-induced pro-inflammatory cytokine production in EAE mice remain largely unclear.In this study,the expression of IL-17,hypoxia inducible factor-1α(HIF-1α),IL-1β,IL-6 and microRNA-497(miR-497),as well as their intrinsic associations,was investigated using EAE model mice and cultured astrocytes exposed to IL-17 in vitro.We observed markedly increased production of IL-17,HIF-1α,IL-1βand IL-6 in the brain tissues of EAE mice,while the expression and secretion of HIF-1α,IL-1βand IL-6 were also significantly increased when cultured primary astrocytes from mice were stimulated with IL-17.Meanwhile,the expression of miR-497 was downregulated both in vivo and in vitro.Subsequent in vitro experiments revealed that IL-17 induced the production of IL-1βand IL-6 in astrocytes through the upregulation of HIF-1αas a transcriptional factor,indicating that IL-17-mediated downregulation of miR-497 enhanced HIF-1αexpression.Furthermore,astrocytespecific knockdown of IL-17RA and HIF-1αor astrocyte-specific overexpression of miR-497 by infection with different lentiviral vectors containing an astrocyte-specific promotor markedly decreased IL-1βand IL-6 production in brain tissues and alleviated the pathological changes and score of EAE mice.Collectively,these findings indicate that decreased miR-497 expression is responsible for IL-17-triggered high HIF-1αexpression and consequent IL-1βand IL-6 production by astrocytes in EAE mice. | Kai Shan Rongrong Pang Chenhui Zhao Xiaomei Liu Wenxing Gao Jing Zhang Dan Zhao Yingwei Wang Wen Qiu | 2017 | Cellular & Molecular Immunology2017，14，11： | 5 |
| 18 | Pyrrolidine dithiocarbamate protects pancreatic β-cells from oxidative damage through regulation of FoxO1 activity in type 2 diabetes rats显示文摘Pyrrolidine dithiocarbamate (PDTC ) 罐头降低血葡萄糖水平并且在糖尿病的 rats.However 改进胰岛素敏感,在糖尿病的老鼠位于 PDTC 治疗的这效果下面的机制仍然保持不明确。在这研究,我们 PDTC 与 experimentaltype 在老鼠对胰腺的小岛房间的氧化损坏由授与保护的 evaluatedthe 机制 2 糖尿病 mellitus (DM ) 。在老鼠的 DM 被伴有单个 intraperitoneal (i.p ) 的长期的高脂肪的饮食得到 streptozotocin 的低剂量的注射。在 PDTC (50 mg/kg/day i.p ) 的一个 7 天的政府以后,血 glucoselevels 被测量,胰腺的纸巾为各种各样的生物化学、酶的 activitiesusing immunohistochemistry, immunofluorescence,和西方的污点技术的决心是镇定的。apoptotic 的百分比胰腺的小岛房间被流动 cytometry 检测。结果证明糖尿病的老鼠提高了血葡萄糖层次和 insulinresistance,随 malondialdehyde 内容, nitrotyrosine 生产,和可诱导的氮的 oxidesynthase 表示的增加伴随了。在 superoxide dismutase 和谷胱甘肽 peroxidase 活动的减少也在 DM 老鼠被观察,与提高的房间 apoptosis 达到顶点。PDTC 处理显著地减少了氧化损坏和房间 apoptosis,并且也通过下面调整的 FoxO1 acetylation 和建议的起来调整的原子 PDX-1 level.These 数据增加了胰岛素生产那 PDTC 能保护小岛房间免受氧化损坏的伤害并且在一个 DM 老鼠模型通过 regulationof PDX-1 和 FoxO1 改进胰岛素生产。 | Haiyan Ding Tienian Zhu Xiaomei Yin Jiankun Liu Lizhong Zhang Michel Bemier Ruijing Zhao | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 4 |
| 19 | Aquaculture area extraction and vulnerability assessment in Sanduao based on richer convolutional features network model显示文摘Sanduao is an important sea-breeding bay in Fujian,South China and holds a high economic status in aquaculture.Quickly and accurately obtaining information including the distribution area,quantity,and aquaculture area is important for breeding area planning,production value estimation,ecological survey,and storm surge prevention.However,as the aquaculture area expands,the seawater background becomes increasingly complex and spectral characteristics differ dramatically,making it difficult to determine the aquaculture area.In this study,we used a high-resolution remote-sensing satellite GF-2 image to introduce a deep-learning Richer Convolutional Features(RCF)network model to extract the aquaculture area.Then we used the density of aquaculture as an assessment index to assess the vulnerability of aquaculture areas in Sanduao.The results demonstrate that this method does not require land and water separation of the area in advance,and good extraction can be achieved in the areas with more sediment and waves,with an extraction accuracy>93%,which is suitable for large-scale aquaculture area extraction.Vulnerability assessment results indicate that the density of aquaculture in the eastern part of Sanduao is considerably high,reaching a higher vulnerability level than other parts. | LIU Yueming YANG Xiaomei WANG Zhihua LU Chen LI Zhi YANG Fengshuo | 2019 | Journal of Oceanology and Limnology2019，37，6： | 4 |
| 20 | Effects of electroacupuncture on c-Fos expression in the spinal cord and brain of rats with chronic visceral hypersensitivity显示文摘BACKGROUND:Visceral hypersensitivity is the main cause of irritable bowel syndrome.c-Fos is a marker of visceral hypersensitivity in the central nervous system.Electroacupuncture can relieve chronic visceral hypersensitivity in rats,but the mechanism is still unknown. OBJECTIVE:To identify c-Fos expression in the spinal cord and cerebral cortex of rats with chronic visceral hypersensitivity,and to test the effects of electroacupuncture on pain sensitivity in rats with chronic visceral hypersensitivity. DESIGN,TIME AND SETTING:A randomized controlled animal experiment was performed at the Animal Experimental Center,Shanghai University of Traditional Chinese Medicine,from January to April,2007. MATERIALS:A total of 24 neonatal,male,Sprague Dawley rats,aged five days old,were equally and randomly assigned into a normal group,a model group,and an electroacupuncture group. Rabbit anti-rat c-Fos antibody and Evision secondary antibody kits(Sigma,USA),diaminobenzidine kit(Dako,Denmark),and an LD202H electroacupuncture apparatus(Huawei,Beijing,China) were used in this study. METHODS:Neonatal rats from the model and electroacupuncture groups were used to establish rat models of chronic visceral hypersensitivity by the saccule stimulation method.After model establishment,0.25 mm diameter electric needles were inserted into Tianshu(ST 25) and Shangjuxu(ST37) at a depth of approximately 0.5 cm,with an square wave(alternating current frequency at 100/20 Hz,amplitude ranged 0.2-0.6 ms,intensity at 1 mA) once for 20 minutes,once a day,for seven days.Rats in the normal and model groups were not treated. MAIN OUTCOME MEASURES:Following 7 days of treatment,c-Fos expression in the spinal cord and cerebral cortex was detected by immunohistochemistry.After the first electroacupuncture treatment,abdominal withdrawal reflex scores were investigated to evaluate the pain threshold for chronic visceral hypersensitivity in rats. RESULTS:Visceral hypersensitivity increased c-Fos staining(P<0.05),and electroacupuncture significantly decreased the number of these cells to near normal levels(P>0.05).Abdominal withdrawal reflex scores were significantly lower in the electroacupuncture and normal groups than in the model group(P<0.05) and were similar between the electroacupuncture and normal groups (P>0.05). CONCLUSION:Electroacupuncture decreases c-Fos expression in the spinal cord and cerebral cortex and increases pain threshold in a chronic visceral hypersensitivity model in rats. | Xiaomei Wang Huirong Liu Guanghong Ding Yunfei Chen Huangan Wu Na Li Enhua Zhou Xiudi Qin Lingsong Yuan | 2009 | Neural Regeneration Research2009，4，5： | 4 |
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