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| 1 | GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity显示文摘Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases. | Qin Chen Peiliang Shi Yufang Wang Dayuan Zou Xiuwen Wu Dingyu Wang Qiongyuan Hu Yujie Zou Zan Huang Jianan Ren Zhaoyu Lin Xiang Gao | 2019 | Journal of Molecular Cell Biology2019，11，6： | 22 |
| 2 | Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation显示文摘Disrupted mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) generation are often associated with macrophage pyroptosis. It remains unclear how these forms of mitochondrial dysfunction relate to inflammasome activation and gasdermin-D (Gsdmd) cleavage, two central steps of the pyroptotic process. Here, we also found MMP collapse and ROS generation induced by Nlrp3 inflammasome activation as previous studies reported. The elimination of ROS alleviated the cleavage of Gsdmd, suggesting that Gsdmd cleavage occurs downstream of ROS release. Consistent with this result, hydrogen peroxide treatment augmented the cleavage of Gsdmd by caspase-1. Indeed, four amino acid residues of Gsdmd were oxidized under oxidative stress in macrophages. The efficiency of Gsdmd cleavage by inflammatory caspase-1 was dramatically reduced when oxidative modification was blocked by mutation of these amino acid residues. These results demonstrate that Gsdmd oxidation serves as a de novo mechanism by which mitochondrial ROS promote Nlrp3 inflammasome-dependent pyroptotic cell death. | Yufang Wang Peiliang Shi Qin Chen Zan Huang Dayuan Zou Jingzi Zhang Xiang Gao Zhaoyu Lin | 2019 | Journal of Molecular Cell Biology2019，11，12： | 23 |
| 3 | The ChinaMAP analytics of deep whole genome sequences in 10,588 individuals显示文摘Metabolic diseases are the most common and rapidly growing health issues worldwide.The massive population-based human genetics is crucial for the precise prevention and intervention of metabolic disorders.The China Metabolic Analytics Project(ChinaMAP)is based on cohort studies across diverse regions and ethnic groups with metabolic phenotypic data in China.Here,we describe the centralized analysis of the deep whole genome sequencing data and the genetic bases of metabolic traits in 10,588 individuals from the ChinaMAP.The frequency spectrum of variants,population structure,pathogenic variants and novel genomic characteristics were analyzed.The individual genetic evaluations of Mendelian diseases,nutrition and drug metabolism,and traits of blood glucose and BMI were integrated.Our study establishes a large-scale and deep resource for the genetics of East Asians and provides opportunities for novel genetic discoveries of metabolic characteristics and disorders. | Yanan Cao Lin Li Min Xu Zhimin Feng Xiaohui Sun Jieli Lu Yu Xu Peina Du Tiange Wang Ruying Hu Zhen Ye Lixin Shi Xulei Tang Li Yan Zhengnan Gao Gang Chen Yinfei Zhang Lulu Chen Guang Ning Yufang Bi Weiqing Wang The ChinaMAP Consortium | 2020 | Cell Research2020，30，9： | 20 |
| 4 | Effects of arbuscular mycorrhizal fungi inoculation on arsenic accumulation by tobacco (Nicotiana tabacum L.)显示文摘A pot experiment was conducted to study the effects of arbuscular mycorrhizal(AM) fungi(from contaminated or uncontaminated soils) on arsenic(As) uptake of tobacco(Nicotiana tabacum L.) in As-contaminated soil.Mycorrhizal colonization rate,dry weight,As and P uptake by plants,concentrations of water-extractable As and As fractions were determined.A low mycorrhizal colonization rate(<25%) was detected.Our research indicated that AM fungi isolated from polluted soils were no more effective than those from unpolluted soils when grown in symbiosis with tobacco.No significant differences were observed in roots and stalks dry weights among all treatments.Leaves and total plant dry weights were much higher in Glomus versiforme treatment than that in control treatment.As contents in roots and stalks from mycorrhizal treatments were much lower than that from control treatment.Total plant As content exhibited the same trend.P concentrations in tobacco were not affected by colonization,nor were stalks,leaves and total plant P contents.Roots P contents were remarkably lower in HN treatments than in other treatments.Meanwhile,decreased soil pH and lower water-extractable As concentrations and higher levels of As fraction bound to well-crystallized hydrous oxides of Fe and Al were found in mycorrhizal treatments than in controls.The protective effect of mycorrhiza against plant As uptake may be associated with changes in As solubility mediated by changing soil pH.These results indicated that under As stress,proper mechanisms employed by AM fungi can protect tobacco against As uptake.Results confirmed that AM fungi can play an important role in food quality and safety. | HUA Jianfeng LIN Xiangui YIN Rui JIANG Qian SHAO Yufang | 2009 | Journal of Environmental Sciences2009，21，9： | 19 |
| 5 | Serum uric acid and risk of incident diabetes in middle-aged and elderly Chinese adults: prospective cohort study显示文摘The association between serum uric acid and the risk of incident diabetes in Chinese adults remains unknown.This study aimed to investigate this association in a community-dwelling population aged≥40 years in Shanghai,China.Oral glucose tole3rance test was conducted during baseline and follow-up visits.Relative risk regression was utilized to examine the associations between baseline gender-specific serum uric acid levels and incident diabetes risk.A total of 613(10.3%)incident diabetes cases were identified during the follow-up visit after 4.5 years.Fasting plasma glucose,postload glucose,and glycated hemoglobin A1c during the follow-up visit progressively increased across the sex-specific quartiles of serum uric acid(all Ps<0.05).The incidence rate of diabetes increased across the quartiles of serum uric acid(7.43%,8.77%,11.47%,and 13.43%).Multivariate adjusted regression analysis revealed that individuals in the highest quartile had 1.36-fold increased risk of diabetes compared with those in the lowest quartile of serum uric acid(odds ratio(95%confidence interval)=1.36(1.06−1.73)).Stratified analysis indicated that the association was only observed in women.Accordingly,serum uric acid was associated with the increased risk of incident diabetes among middle-aged and elderly Chinese women. | Di Cheng Chunyan Hu Rui Du Hongyan Qi Lin Lin Xueyan Wu Lina Ma Kui Peng Mian Li Min Xu Yu Xu Yufang Bi Weiqing Wang Yuhong Chen Jieli Lu | 2020 | Frontiers of Medicine2020，14，6： | 3 |
| 6 | Molecular identification of Echinococcus species from eastern and southern Qinghai, China, based on the mitochondrial cox1 gene显示文摘 | Junying Ma Hu Wang Gonghua Lin Philip S. Craig Akira Ito Zhenyuan Cai Tongzuo Zhang Xiumin Han Xiao Ma Jingxiao Zhang Yufang Liu Yanmei Zhao Yongshun Wang | 2012 | Parasitology Research2012，，1： | 2 |
| 7 | The ChinaMAP reference panel for the accurate genotypeimputation in Chinese populations显示文摘Dear Editor,The genotype imputation is an efficient and pivotal approach to estimate the unobserved genotypes in the genomic data from the single nucleotide polymorphism(SNP)genotyping arrays or whole-genome sequencing(WGS).The fine mapping of variants in the genome-wide association study(GWAS)could be critically improved by the imputation with an optimal reference panel based on the population-specific haplotypes. | Lin Li Peide Huang Xiaohui Sun Siyu Wang Min Xu Sha Liu Zhimin Feng Qing Zhang Xiaoji Wang Xiaole Zheng Mengyao Dai Yufang Bi Guang Ning Yanan Cao Weiqing Wang | 2021 | Cell Research2021，31，12： | 2 |
| 8 | Capsid destabilization and epitope alterations of human papillomavirus 18 in the presence of thimerosal显示文摘Thimerosal has been widely used as a preservative in drug and vaccine products for decades.Due to the strong propensity to modify thiols in proteins,conformational changes could occur due to covalent bond formation between ethylmercury(a degradant of thimerosal)and thiols.Such a conformational change could lead to partial or even complete loss of desirable protein function.This study aims to investigate the effects of thimerosal on the capsid stability and antigenicity of recombinant human papillomavirus(HPV)18 virus-like particles(VLPs).Dramatic destabilization of the recombinant viral capsid upon thimerosal treatment was observed.Such a negative effect on the thermal stability of VLPs preserved with thimerosal was shown to be dependent on the thimerosal concentration.Two highly neutralizing antibodies,13H12 and 3C3,were found to be the most sensitive to thimerosal treatment.The kinetics of antigenicity loss,when monitored with 13H12 or 3C3 as probes,yielded two distinctly different sets of kinetic parameters,while the data from both monoclonal antibodies(mAbs)followed a biphasic exponential decay model.The potential effect of thimerosal on protein function,particularly for thiolcontaining proteinaceous active components,needs to be comprehensively characterized during formulation development when a preservative is necessary. | Xiaofen Huang Yike Li Meifeng Nie Mingxi Yue Yufang Li Zhijie Lin Huirong Pan Mujin Fang Ting Wu Shaowei Li Jun Zhang Ningshao Xia Qinjian Zhao | 2021 | Journal of Pharmaceutical Analysis2021，11，5： | 1 |
| 9 | Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors显示文摘 | Bing Yu Zhi-Ying He Pu You Qing-Wang Han Dao Xiang Fei Chen Min-Jun Wang Chang-Cheng Liu Xi-Wen Lin Uyunbilig Borjigin Xiao-Yuan Zi Jian-Xiu Li Hai-Ying Zhu Wen-Lin Li Chun-Sheng Han Kirk J. Wangensteen Yufang Shi Li-Jian Hui Xin Wang Yi-Ping Hu | 2013 | Cell Stem Cell2013，，： | 1 |
| 10 | Length of the ORF, position of the first AUG and the Kozak motif are important factors in potential dual-coding transcripts显示文摘一个单个哺乳动物的抄本通常编码一蛋白质,但是 GNAS (G 蛋白质伪 - 子单元) 的抄本包含二个读物框架并且生产二在结构上无关的蛋白质, XL 伪 s 和 ALEX。没有其它证实象 GNAS 一样双编码的抄本迄今为止被报导了,尽管许多如此的候选人基因被生物信息学分析预言了。在这研究,我们构造了一系列向量测试二个蛋白质产品怎么在 vitro 从一个单个抄本被翻译。ORF (开的读框架) 的长度,第一 8 月的位置和 Kozak 主题被发现是重要因素。这些因素,以及 55-bp NMD (调停胡说八道的 mRNA 腐烂) 规则,为候选人在生物信息学搜索被使用双编码的抄本。分别地, 1307, 750 和 474 个 two-ORF-containing 抄本的一个总数分别地, 170, 89 和 70 哪个被发现是潜在的双编码的抄本在人,老鼠和老鼠被发现。大多数抄本在种类之中显示出低保存。有趣地,双编码的抄本显著地从锌手指蛋白质家庭为抄本被充实,它通常是涉及抄写过程的规定的 DNA 有约束力的蛋白质。 | Heng Xu Ping Wang Yujie Fu Yufang Zheng Quan Tang Lizhen Si Jin You Zhenguo Zhang Yufei Zhu Li Zhou Zejun Wei Bin Lin Landian Hu Xiangyin Kong | 2010 | Cell Research2010，20，4： | 1 |
| 11 | Age-related cardiac muscle sarcopenia:Combining experimental and mathematical modeling to identify mechanisms显示文摘 | Jing Lin Elizabeth F Lopez Jin Yufang | 2008 | Experimental Gerontology2008，43，4： | 1 |
| 12 | Preparation, struc- ture and properties of nitrile-butadiene rubber-organoelay nano-composites by reactive mixing intercalation method 显示文摘 | Liu Lin Jia Deming Luo Yufang | 2006 | Ap- plied Polymer Science2006，100，3： | 1 |
| 13 | Tolerance to freezing stress in cyanobacteria, Nostoc commune and some cyanobacteria with various tolerances to drying stress显示文摘 | Lin Yufang Hirai M Kashino Y | 2004 | National Institute of Polar Research2004，17，： | 1 |
| 14 | Tolerance tofreezing stress in cyanobacteria, Nostoc commune and some cyanobacteria with various tolerances to drying stress 显示文摘 | LIN Yufang HIRAI M KASHINO Y | 2004 | National Institute of Polar Research2004，17，： | 1 |
| 15 | In vitro selection of glyphosate-tolerant variants from long-term callus cultures of Zoysia matrella [L.] Merr显示文摘 | Shu Chen Mingliang Chai Yufang Jia Zhongshan Gao Li Zhang Minxia Gu Wendan Lin Liping Wang | 2012 | Plant Cell Tissue and Organ Culture (PCTOC)2012，，2： | 1 |
| 16 | Studies on Fe80B20 and Fe82B18 -xSix amorphous alloys显示文摘 | Zheng Yufang Lin Deming Yu Zhengfang | 1985 | ACTA Scientarum Naturalium Universitatis Sunyatseni1985，，1： | 1 |
| 17 | Beneficial effects of benzodiazepine diazepam on chronic stress-induced impairment of hippocampal structural plasticity and depression-like behavior in mice显示文摘 | Yunan Zhao Zhongli Wang Jianguo Dai Lin Chen Yufang Huang Zhen Zhan | 2011 | Behavioural Brain Research2011，，2： | 1 |
| 18 | Fast Zn^(2+)mobility enabled by sucrose modified Zn^(2+)solvation structure for dendrite-free aqueous zinc battery显示文摘Aqueous zinc battery has been regarded as one of the most promising energy storage systems due to its low cost and environmental benignity.However,the safety concern on Zn anodes caused by uncontrolled Zn dendrite growth in aqueous electrolyte hinders their application.Herein,sucrose with multi-hydroxyl groups has been introduced into aqueous electrolyte to modify Zn^(2+)solvation environment and create a protection layer on Zn anode,thus effectively retarding the growth of zinc dendrites.Atomistic simulations and experiments confirm that sucrose molecules can enter into the solvation sheath of Zn^(2+),and the as-formed unique solvation structure enhances the mobility of Zn^(2+).Such fast Zn^(2+)kinetics in sucrose-modified electrolyte can successfully suppress the dendrite growth.With this sucrose-modified aqueous electrolyte,Zn/Zn symmetric cells present more stable cycle performance than those using pure aqueous electrolyte;Zn/C cells also deliver an impressive higher energy density of 129.7 Wh·kg^(−1)and improved stability,suggesting a great potential application of sucrose-modified electrolytes for future Zn batteries. | Yufang Cao Xiaohui Tang Linge Li Haifeng Tu Yuzhen Hu Yingying Yu Shuang Cheng Hongzhen Lin Liwen Zhang Jiangtao Di Yongyi Zhang Meinan Liu | 2023 | Nano Research2023，16，3： | 0 |
| 19 | MAPK4 facilitates angiogenesis by inhibiting the ERK pathway in non-small cell lung cancer显示文摘Background:Angiogenesis plays an important role in the occurrence and development of non-small cell lung cancer(NSCLC).The atypical mitogen-activated protein kinase 4(MAPK4)has been shown to be involved in the pathogenesis of various diseases.However,the potential role of MAPK4 in the tumor angiogenesis of NSCLC remains unclear.Methods:Adult male C57BL/6 wild-type mice were randomly divided into the control group and p-siMAPK4 intervention group,respectively.The cell proliferation was analyzed with flow cytometry and immunofluorescence staining.The vascular density in tumor mass was analyzed by immuno-fluorescence staining.The expressions of MAPK4 and related signaling molecules were detected by western blot analysis and immunofluorescence staining,and so on.Results:We found that the expression of MAPK4,which was dominantly expressed in local endothelial cells(ECs),was correlated with tumor angiogenesis of NSCLC.Furthermore,MAPK4 silencing inhibited the proliferation and migration abilities of human umbilical vein ECs(HUVECs).QKHZC-2020-4Y156,QKH-JC-2018-1428,QKH-RC-2019-5612;Collaborative Innovation Center of Chinese Ministry of Education,Grant/Award Number:2020-39;Program for Science and Technology Joint Fund Project in Zunyi Science and Technology Bureau and Zunyi Medical University,Grant/Award Numbers:ZSKH-RPT-2020-6,ZSKH-HZ-2021-193;Graduate Research Fund of Zunyi Medical University,Grant/Award Number:2023-ZYK-171 Global gene analysis showed that MAPK4 silencing altered the expression of multiple genes related to cell cycle and angiogenesis pathways,and that MAPK4 silencing increased transduction of the extracellular regulated protein kinases 1/2(ERK1/2)pathway but not Akt and c-Jun n-terminal kinase pathways.Further analysis showed that MAPK4 silencing inhibited the proliferation and migration abilities of HUVECs cultured in tumor cell supernatant,which was accompanied with increased transduction of the ERK1/2 pathway.Clinical data analysis suggested that the higher expression of MAPK4 and CD34 were associated with poor prognosis of patients with NSCLC.Targeted silencing of MAPK4 in ECs using small interfering RNA driven by the CD34 promoter effectively inhibited tumor angiogenesis and growth of NSCLC in vivo.Conclusion:Our results reveal that MAPK4 plays an important role in the angiogenesis and development of NSCLC.MAPK4 may thus represent a new target for NSCLC. | Jing Chen Jing Yang Yufang Liu Xu Zhao Juanjuan Zhao Lin Tang Mengmeng Guo Ya Zhou Chao Chen Dongmei Li Zhenke Wen Guiyou Liang Lin Xu | 2024 | Cancer Innovation2024，3，3： | 0 |
| 20 | Cardiopulmonary prognosis of prophylactic endotracheal intubation in patients with upper gastrointestinal bleeding undergoing endoscopy显示文摘BACKGROUND:It is controversial whether prophylactic endotracheal intubation(PEI)protects the airway before endoscopy in critically ill patients with upper gastrointestinal bleeding(UGIB).The study aimed to explore the predictive value of PEI for cardiopulmonary outcomes and identify high-risk patients with UGIB undergoing endoscopy.METHODS:Patients undergoing endoscopy for UGIB were retrospectively enrolled in the eICU Collaborative Research Database(eICU-CRD).The composite cardiopulmonary outcomes included aspiration,pneumonia,pulmonary edema,shock or hypotension,cardiac arrest,myocardial infarction,and arrhythmia.The incidence of cardiopulmonary outcomes within 48 h after endoscopy was compared between the PEI and non-PEI groups.Logistic regression analyses and propensity score matching analyses were performed to estimate effects of PEI on cardiopulmonary outcomes.Moreover,restricted cubic spline plots were used to assess for any threshold effects in the association between baseline variables and risk of cardiopulmonary outcomes(yes/no)in the PEI group.RESULTS:A total of 946 patients were divided into the PEI group(108/946,11.4%)and the non-PEI group(838/946,88.6%).After propensity score matching,the PEI group(n=50)had a higher incidence of cardiopulmonary outcomes(58.0%vs.30.3%,P=0.001).PEI was a risk factor for cardiopulmonary outcomes after adjusting for confounders(odds ratio[OR]3.176,95%confidence interval[95%CI]1.567-6.438,P=0.001).The subgroup analysis indicated the similar results.A shock index>0.77 was a predictor for cardiopulmonary outcomes in patients undergoing PEI(P=0.015).The probability of cardiopulmonary outcomes in the PEI group depended on the Charlson Comorbidity Index(OR 1.465,95%CI 1.079-1.989,P=0.014)and shock index>0.77(compared with shock index≤0.77[OR 2.981,95%CI 1.186-7.492,P=0.020,AUC=0.764]).CONCLUSION:PEI may be associated with cardiopulmonary outcomes in elderly and critically ill patients with UGIB undergoing endoscopy.Furthermore,a shock index greater than 0.77 could be used as a predictor of a worse prognosis in patients undergoing PEI. | Yufang Lin Fei’er Song Weiyue Zeng Yichi Han Xiujuan Chen Xuanhui Chen Yu Ouyang Xueke Zhou Guoxiang Zou Ruirui Wang Huixian Li Xin Li | 2023 | World Journal of Emergency Medicine2023，14，5： | 0 |
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