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| 1 | Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury显示文摘The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other countries.Here we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,China.All 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 count.The viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease severity.ALB,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung injury.Age,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease severity.Moreover,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung injury.Our results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection. | Yingxia Liu Yang Yang Cong Zhang Fengming Huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu | 2020 | Science China(Life Sciences)2020，63，3： | 157 |
| 2 | Preface显示文摘This special issue of Acta Mathematica Scientia is dedicated to the celebration of the 70th birthday of Constantine M. Dafermos. Costas is the Alumni-Alumnae University Professor of Brown University, a Fellow of the American Academy of Arts and Sciences, | Chen, Gui-Qiang G. Hsiao, Ling Huang, Feimin Tzavaras, Athanasios Wang, Zhen | 2012 | Acta Mathematica Scientia2012，32，1： | 72 |
| 3 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 4 | Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis显示文摘Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method. | SUN YUE WEI ZHANG FENG LING LI YING LI GUO TIAN LEI LIU HAI HUANG | 2000 | Cell Research2000，10，4： | 53 |
| 5 | Persistence and clearance of viral RNA in 2019 novel coronavirus disease rehabilitation patients显示文摘Background:A patient’s infectivity is determined by the presence of the virus in different body fluids,secretions,and excreta.The persistence and clearance of viral RNA from different specimens of patients with 2019 novel coronavirus disease(COVID-19)remain unclear.This study analyzed the clearance time and factors influencing 2019 novel coronavirus(2019-nCoV)RNA in different samples from patients with COVID-19,providing further evidence to improve the management of patients during convalescence.Methods:The clinical data and laboratory test results of convalescent patients with COVID-19 who were admitted to from January 20,2020 to February 10,2020 were collected retrospectively.The reverse transcription polymerase chain reaction(RT-PCR)results for patients’oropharyngeal swab,stool,urine,and serum samples were collected and analyzed.Convalescent patients refer to recovered non-febrile patients without respiratory symptoms who had two successive(minimum 24 h sampling interval)negative RT-PCR results for viral RNA from oropharyngeal swabs.The effects of cluster of differentiation 4(CD4)+T lymphocytes,inflammatory indicators,and glucocorticoid treatment on viral nucleic acid clearance were analyzed.Results:In the 292 confirmed cases,66 patients recovered after treatment and were included in our study.In total,28(42.4%)women and 38 men(57.6%)with a median age of 44.0(34.0-62.0)years were analyzed.After in-hospital treatment,patients’inflammatory indicators decreased with improved clinical condition.The median time from the onset of symptoms to first negative RT-PCR results for oropharyngeal swabs in convalescent patients was 9.5(6.0-11.0)days.By February 10,2020,11 convalescent patients(16.7%)still tested positive for viral RNA from stool specimens and the other 55 patients’stool specimens were negative for 2019-nCoV following a median duration of 11.0(9.0-16.0)days after symptom onset.Among these 55 patients,43 had a longer duration until stool specimens were negative for viral RNA than for throat swabs,with a median delay of 2.0(1.0-4.0)days.Results for only four(6.9%)urine samples were positive for viral nucleic acid out of 58 cases;viral RNA was still present in three patients’urine specimens after throat swabs were negative.Using a multiple linear regression model(F=2.669,P=0.044,and adjusted R2=0.122),the analysis showed that the CD4+T lymphocyte count may help predict the duration of viral RNA detection in patients’stools(t=-2.699,P=0.010).The duration of viral RNA detection from oropharyngeal swabs and fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(15 days vs.8.0 days,respectively;t=2.550,P=0.013)and the duration of viral RNA detection in fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(20 days vs.11 days,respectively;t=4.631,P<0.001).There was no statistically significant difference in inflammatory indicators between patients with positive fecal viral RNA test results and those with negative results(P>0.05).Conclusions:In brief,as the clearance of viral RNA in patients’stools was delayed compared to that in oropharyngeal swabs,it is important to identify viral RNA in feces during convalescence.Because of the delayed clearance of viral RNA in the glucocorticoid treatment group,glucocorticoids are not recommended in the treatment of COVID-19,especially for mild disease.The duration of RNA detection may relate to host cell immunity. | Yun Ling Shui-Bao Xu Yi-Xiao Lin Di Tian Zhao-Qin Zhu Fa-Hui Dai Fan Wu Zhi-Gang Song Wei Huang Jun Chen Bi-Jie Hu Sheng Wang En-Qiang Mao Lei Zhu Wen-Hong Zhang Hong-Zhou Lu | 2020 | Chinese Medical Journal2020，，9： | 58 |
| 6 | Incidence trend of nasopharyngeal carcinoma from 1987 to 2011 in Sihui County,Guangdong Province,South China:an age-period-cohort analysis显示文摘Introduction:In the past several decades,declining incidences of nasopharyngeal carcinoma(NPC) have been observed in Chinese populations in Hong Kong,Taiwan,Los Angeles,and Singapore.A previous study indicated that the incidence of NPC in Sihui County,South China remained stable until 2002,but whether age,diagnosis period,and birth cohort affect the incidence of NPC remains unknown.Methods:Age-standardized rates(ASRs) of NPC incidence based on the world standard population were examined in both males and females in Sihui County from 1987 to 2011.Joinpoint regression analysis was conducted to quantify the changes in incidence trends.A Poisson regression age-period-cohort model was used to assess the effects of age,diagnosis period,and birth cohort on the risk of NPC.Results:The ASRs of NPC incidence during the study period were 30.29/100,000 for males and 13.09/100,000 for females.The incidence of NPC remained stable at a non-significant average annual percent change of 0.2%for males and-1.6%for females throughout the entire period.A significantly increased estimated annual percent change of 6.8%(95%confidence interval,0.1%-14.0%) was observed from 2003 to 2009 for males.The relative risk of NPC increased with advancing age up to 50-59 and decreased at ages >60 years.The period effect curves on NPC were nearly flat for males and females.The birth cohort effect curve for males showed an increase from the1922 cohort to the 1957 cohort and a decrease thereafter.In females,there was an undulating increase in the relative risk from the 1922 cohort to the 1972 cohort.Conclusions:The incidence trends for NPC remained generally stable in Sihui from 1987 to 2011,with an increase from 2003 to 2009.The relative risks of NPC increased in younger females. | Li-Fang Zhang Yan-Hua Li Shang-Hang Xie Wei Ling Sui-Hong Chen Qing Liu Qi-Hong Huang Su-Mei Cao | 2015 | Chinese Journal of Cancer2015，34，8： | 47 |
| 7 | Quantitative characterization of reservoir space in the Lower Silurian Longmaxi Shale,southern Sichuan,China显示文摘Based on the drilling data of the Upper Ordovician Wufeng Shale and the Lower Silurian Longmaxi Shale in southern Sichuan Basin,the construction of matrix pores and the development condition of fractures in a marine organic-rich shale are quantitatively evaluated through the establishment of the reservoir petrophysical models and porosity mathematical models.Our studies show that there are four major characteristics of the Longmaxi Shale confirmed by the quantitative characterization:(1)the pore volume of per unit mass is the highest in organic matter,followed in clay minerals,finally in brittle minerals;(2)the porosity of the effective shale reservoir is moderate and equal to that of the Barnett Shale,and the main parts of the shale reservoir spaces are interlayer pores of clay minerals and organic pores;(3)the porosity of the organic-rich shale is closely related to TOC and brittle mineral/clay mineral ratio,and mainly increases with TOC and clay mineral content;(4)fractures are developed in this black shale,and are mainly micro ones and medium-large ones.In the Longmaxi Shale,the fracture density increases from top to bottom,reflecting the characteristics with high brittle mineral content,high Young’s modulus,low Poisson's ratio and high brittleness at its bottom. | WANG YuMan DONG DaZhong YANG Hua HE Ling WANG ShiQian HUANG JinLiang PU BoLing WANG ShuFang | 2014 | Science China Earth Sciences2014，57，2： | 36 |
| 8 | Phase II clinical trial using camrelizumab combined with apatinib and chemotherapy as the first-line treatment of advanced esophageal squamous cell carcinoma显示文摘Background:Effective therapeutic options are limited for patients with advanced esophageal squamous cell carcinoma(ESCC).The incorporation of an immune checkpoint inhibitor and a molecular anti-angiogenic agent into the commonly adopted chemotherapy may produce synergistic effects.Therefore,we aimed to investigate the efficacy and safety of camrelizumab plus apatinib combined with chemotherapy as the first-line treatment of advanced ESCC.Methods:In this single-arm prospective phase II trial,patients with unresectable locally advanced or recurrent/metastatic ESCC received camrelizumab 200 mg,liposomal paclitaxel 150 mg/m2,and nedaplatin 50 mg/m2 on day 1,and apatinib 250 mg on days 1-14.The treatments were repeated every 14 days for up to 9 cycles,followed by maintenance therapy with camrelizumab and apatinib.The primary endpoint was objective response rate(ORR)according to the Response Evaluation Criteria in Solid Tumors(version 1.1).Secondary endpoints included disease control rate(DCR),progression-free survival(PFS),overall survival(OS),and safety.Results:We enrolled 30 patients between August 7,2018 and February 23,2019.The median follow-up was 24.98 months(95%confidence interval[CI]:23.05-26.16 months).The centrally assessed ORR was 80.0%(95%CI:61.4%-92.3%),with a median duration of response of 9.77 months(range:1.54 to 24.82+months).The DCR reached 96.7%(95%CI:82.8%-99.9%).The median PFS was 6.85 months(95%CI:4.46-14.20 months),and the median OS was 19.43 months(95%CI:9.93 months–not reached).The most common grade 3-4 treatmentrelated adverse events(AEs)were leukopenia(83.3%),neutropenia(60.0%),and increased aspartate aminotransferase level(26.7%).Treatment-related serious AEs included febrile neutropenia,leukopenia,and anorexia in one patient(3.3%),and single cases of increased blood bilirubin level(3.3%)and toxic epidermal necrolysis(3.3%).No treatment-related deaths occurred.Conclusions:Camrelizumab plus apatinib combined with liposomal paclitaxel and nedaplatin as first-line treatment demonstrated feasible anti-tumor activity and manageable safety in patients with advanced ESCC.Randomized trials to evaluate this new combination strategy are warranted.Trial registration:This trial was registered on July 27,2018,at ClinicalTrials.gov(identifier:NCT03603756). | Bo Zhang Ling Qi Xi Wang Jianping Xu Yun Liu Lan Mu Xingyuan Wang Lidan Bai Jing Huang | 2020 | Cancer Communications2020，40，12： | 38 |
| 9 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 10 | Anti-tumor activities and apoptosis-regulated mechanisms of bufalin on the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice显示文摘AIM: To investigate anti-tumor activities and apoptosis-regulated mechanisms of bufalin in the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice.METHODS: BEL-7402 cells of human hepatocellular carcinoma were inoculated to form subcutaneous tumors, and were implanted into the liver to establish orthotopic transplantation tumor models of human hepatocellular carcinoma in nude mice. Seventy-five animals were randomized divided into five groups (n = 15). Bufalin was injected intraperitoneally into three groups at doses of 1.5 mg/kg (BF1), 1 mg/kg (BF2) and 0.5 mg/kg (BF3) for d 15-24, respectively. The NS group was injected an equal volume of saline as above and adriamycin was injected intraperitoneally into the ADM group at a dose of 8.0 mg/kg for d 15. Ten mice in each group were killed at d 25 and the survival time in each group was calculated. We also observed the morphologic alterations in the myocardium, brain, liver, kidney and tumor tissues by pathology and electron microscopy, measured the apoptotic rate by TUNEL staining method, and detected the expression of apoptosis-regulated genes bcl-2 and bax by immunohistochemical staining and RT-PCR in tumor tissues. RESULTS: The tumor volumes in each group of bufalin were reduced significantly (35.21 ± 12.51 vs 170.39 ± 25.29; 49.83 ± 11.46 vs 170.39 ± 25.29; 83.99 ± 24.63 vs 170.39 ± 25.29, P < 0.01, respectively), and the survival times were prolonged in group BF1-2 (31.8 ± 4.2 vs 23.4 ± 2.1 and 29.4 ± 3.4 vs 23.4 ± 2.1, P < 0.05, respectively), and necrosis was mainly in severe or moderate degree in group BF1-2. No morphologicalchanges were detected in the myocardium, brain, liver and kidney tissues. Apoptotic characteristics could be seen in group BF1-2. The positive rates of bcl-2 and bax protein expression of each group by immunohistochemical staining were 10.0%, 10.0%, 20.0%, 10.0% and 20.0%; 90.0%, 80.0%, 80.0%, 40.0% and 30.0%, respectively. Loss of expression of bcl-2 mRNA in each group was to be found and the density of bax mRNA was increased progressively with increase of dose of bufalin by RT-PCR. CONCLUSION: Bufalin has significant anti-tumor activities in the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice with no marked toxicity and was able to induce apoptosis of transplanted tumor cells. This apoptosis may be mediated mainly via up-regulating the expression of apoptosis-regulated gene bax, which may be involved in its anti-tumor mechanism of bufalin. | Ke-Qi Han Guang Huang Wei Gu Yong-Hua Su Xue-Qiang Huang Chang-Quan Ling | 2007 | World Journal of Gastroenterology2007，13，24： | 32 |
| 11 | Inhibitory effect of recombinant adenovirus carrying melittin gene on hepatocellular carcinoma显示文摘 | Ling CQ Li B Zhang C Zhu DZ Huang XQ Gu W Li SX | 2005 | 第二军医大学学报2005，26，10： | 30 |
| 12 | Predicting lifetime risk for developing atherosclerotic cardiovascular disease in Chinese population:the China-PAR project显示文摘Evidence on the lifetime risk for atherosclerotic cardiovascular disease(ASCVD) is insufficient;yet,estimating an individual's lifetime risk allows for a comprehensive assessment of ASCVD burden.We developed and validated lifetime risk prediction equations for ASCVD using four large and ongoing prospective cohorts of Chinese,the China-PAR project(Prediction for ASCVD Risk in China).Sexspecific equations were developed using two cohorts(as the derivation cohort) of 21,320 participants.Two other independent cohorts with 14,123 and 70,838 participants were used for their external validation,respectively.We evaluated both calibration and discrimination measures for model performance.Furthermore,we estimated ASCVD-free years lost or excess absolute risk attributable to high 10-year risk(!10.0%) and/or high lifetime risk(!32.8%).After 12.3 years' follow-up of the derivation cohort,1048 ASCVD events and 1304 non-ASCVD deaths were identified.Our sex-specific equations had good internal validation,with discriminant C statistics of 0.776(95% confidence interval [CI]:0.757–0.794) and 0.801(95% CI:0.778–0.825),and calibration χ~2 of 9.2(P = 0.418) and 5.6(P = 0.777) for men and women,respectively.Good external validation was also demonstrated with predicted rates closely matched to the observed ones.Compared with men having both low 10-year and low lifetime risk,men would develop ASCVD 3.0,4.6 and 8.6 years earlier if they had high 10-year risk alone,high lifetime risk alone,or both high 10-year and high lifetime risk at the index age of 35 years,respectively.We developed wellperformed lifetime risk prediction equations that will help to identify those with the greatest potential to avert ASCVD burden after implementation of innovative clinical and public health interventions in China. | Fangchao Liu Jianxin Li Jichun Chen Dongsheng Hu Ying Li Jianfeng Huang Xiaoqing Liu Xueli Yang Jie Cao Chong Shen Ling Yu Zhendong Liu Xianping Wu Liancheng Zhao Xigui Wu Dongfeng Gu Xiangfeng Lu | 2018 | Science Bulletin2018，63，12： | 30 |
| 13 | Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin显示文摘LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里,我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响, Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽,我们观察到 E-cadherin 的表示,与肿瘤转移联系的一个必要粘附分子,被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而,抑制被雌激素剥夺废除,显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对,建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。 | Yuan Guang Meng Wei Dong Han Ya Li Zhao Ke Huang Yi Ling Si Zhi Qiang Wu Yi Ming Mu | 2007 | Cell Research2007，17，10： | 27 |
| 14 | Requirement and Functional Redundancy of Ib Subgroup bHLH Proteins for Iron Deficiency Responses and Uptake in Arabidopsis thaliana显示文摘在 Arabidopsis 的 bHLH 基因家庭的 Ib 亚群包含四个成员(AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 ) 。AtbHLH38 和 AtbHLH39 以前被证实与象 FER 一样铁缺乏交往导致的抄写因素(合适) ,直接在 ferricchelate reductase FRO2 和高亲密关系的铁的铁 transporter IRT1 的表示的激活工作。在这个工作,我们在铁缺乏回答和举起的规定描绘了 AtbHLH100 和 AtbHLH101 的功能。酵母二混血儿的分析和双分子的荧光互补试金证明 AtbHLH100 和 AtbHLH101 能交往与合适。AtbHLH100 或 AtbHLH101 与的双表示装入激活的酵母房间 FRO2 和 IRT1 的倡导者驾驶的 GUS 表示。工厂 overexpressing 与 AtbHLH101 结合了在根显示出 FRO2 和 IRT1 的组成的表示,并且在射击积累了更多的铁。进一步, AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 的单个、双、三倍的猛烈异种被产生并且描绘。在根的 FRO2 和 IRT1 表示和在射击的铁内容更急速地比双的可得到的其它和四基因的三倍的异种的在 AtbHLH39, AtbHLH100,和 AtbHLH101 的三倍的猛烈异种被减少。象在在铁缺乏下面的多重猛烈异种的 FRO2 和 IRT1 的表示一样的生理的回答的比较表明 AtbHLH100, AtbHLH38, AtbHLH101,和 AtbHLH39 在铁缺乏回答和举起起了逐渐地增加的重要作用。一起拿所有,我们断定四 Ib 亚群 bHLH 蛋白质在 Arabidopsis 为铁缺乏回答和举起的激活与微分意义被要求并且拥有冗余的功能。 | Ning Wang Yan Cui Yi Liu Huajie Fan Juan Du Zongan Huang Youxi Yuan Huilan Wu Hong-Qing Ling | 2013 | Molecular Plant2013，6，2： | 26 |
| 15 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 16 | The structural and accessory proteins M,ORF 4a,ORF 4b,and ORF 5 of Middle East respiratory syndrome coronavirus(MERS-CoV)are potent interferon antagonists显示文摘The newly emerged Middle East respiratory syndrome coronavirus(MERS-CoV)is a highly pathogenic respira-tory virus with pathogenic mechanisms that may be driven by innate immune pathways.The goal of this study is to characterize the expression of the structural(S,E,M,N)and accessory(ORF 3,ORF 4a,ORF 4b,ORF 5)proteins of MERS-CoV and to determine whether any of these pro-teins acts as an interferon antagonist.Individual structural and accessory protein-coding plasmids with an N-terminal HA tag were constructed and transiently transfected into cells,and their native expression and subcellular localiza-tion were assessed using Wes tern blotting and indirect immunofl uorescence.While ORF 4b demonstrated majorly nuclear localization,all of the other proteins demonstrated cytoplasmic localization.In addition,for the fi rst time,our experiments revealed that the M,ORF 4a,ORF 4b,and ORF 5 proteins are potent interferon antagonists.Further exami-nation revealed that the ORF 4a protein of MERS-CoV has the most potential to counteract the antiviral effects of IFN via the inhibition of both the interferon production(IFN-βpromoter activity,IRF-3/7 and NF-κB activation)and ISRE promoter element signaling pathways.Together,our re-sults provide new insights into the function and pathogenic role of the structural and accessory proteins of MERS-CoV. | Yang Yang Ling Zhang Heyuan Geng Yao Deng Baoying Huang Yin Guo Zhengdong Zhao Wenjie Tan | 2013 | Protein & Cell2013，4，12： | 25 |
| 17 | Heavy metal concentrations of agricultural soils and vegetables from Dongguan, Guangdong显示文摘118 农业土壤和 43 件蔬菜样品的一个总数从 Dongguan 城市被收集,广东,中国。空间分发,来源,累积特征和在农业土壤和蔬菜的重金属的潜在的风险被三条不同途径在细节描绘,包括在土壤和蔬菜的八个金属元素的全部的内容,在在学习的土壤的重金属的 GIS 地图和 multivariate 分析。结果证明在农业土壤有象 Cu , Zn , Ni , Pb , Cd 和 Hg 那样的重金属的更高的累积,并且 Pb 的内容( 65.38 mg kg ? 2 )并且 Hg ( 0.24 mg kg ? 2 )分别地是在广东省的土壤的相应重金属的背景内容的 1.82 和 2.82 次。有在附近的 3.4% Cu, 5.9% Ni, 1.7% Cd 和 28% Hg 在所有从有的所有调查地点收集了土壤样品溢出为为土壤标准的中国环境质量的重金属的内容(GB15618-1995,等级 II ) 。在土壤的多金属的污染特征被 Hg 主要反映。到在土壤的八个金属元素有不同来源, Cu, Zn, Ni, Cr 并且这是主要源于父母材料,并且 Pb, Hg 和 Cd 被人为的活动影响。空间分发表演 Cu, Zn, Ni, Cr, Pb 并且农业土壤的 Hg 内容在西方高并且在东方,和 Cd 内容低在西北高,东南并且在在 Dongguan 的西南低。Ni 的蔬菜样品的比率, Pb 并且作为比最大值高的集中,在食物(GB2762-2005 ) 的沾染物的层次分别地是 4.7% , 16.3% 和 48.8% 。在蔬菜的重金属的简历集中因素(BCF ) 的顺序是 Cd > Zn > Cu > 作为 > Ni > Hg > Cr > Pb。在 Dongguan 城市里从农业土壤和蔬菜为食物安全和人健康集中于重金属的潜在的风险是必要的,广东省。 | CAI Limei HUANG Lanchun ZHOU Yongzhang XU Zhencheng PENG Xiaochun YAO Ling'ai ZHOU Yang PENG Ping'an | 2010 | Journal of Geographical Sciences2010，20，1： | 23 |
| 18 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 19 | Cell–cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models显示文摘Mesenchymal stem cells(MSCs),which are pluripotent cells with immunomodulatory properties,have been considered good candidates for the therapy of several immune disorders,such as inflammatory bowel diseases,concanavalin A-induced liver injury,and graft-versus-host disease.The embryo is a natural allograft to the maternal immune system.A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation,followed by the switch to a tolerant immune microenvironment in both the uterus and the system.Excessive infiltration of immune cells and serious inflammatory responses are triggers for embryo rejection,which results in miscarriage.Here,we demonstrated that adoptive transfer of MSCs could prevent fetal loss in a lipopolysaccharide(LPS)-induced abortion model and immune response-mediated spontaneous abortion model.The immunosuppressive MSCs alleviated excessive inflammation by inhibiting CD4+T cell proliferation and promoting the decidual macrophage switch to M2 in a tumor necrosis factor-stimulated gene-6(TSG-6)-dependent manner.Cell-tocell contact with proinflammatory macrophages increased the TSG-6 production by the MSCs,thereby enhancing the suppressive regulation of T cells and macrophages.Moreover,proinflammatory macrophages in contact with the MSCs upregulated the expression of CD200 on the stem cells and facilitated the reprogramming of macrophages towards an anti-inflammatory skew through the interaction of CD200 with CD200R on proinflammatory macrophages.Therefore,the results demonstrate that a TSG-6-mediated paracrine effect,reinforced by cell-to-cell contact between MSCs and proinflammatory macrophages,is involved in the mechanism of MSC-mediated abortion relief through the induction of immune tolerance.Our study also indicates the potential application of MSCs in clinical recurrent miscarriages. | Yanhong Li Di Zhang Ling Xu Lin Dong Ji Zheng Yikong Lin Jiefang Huang Yanyun Zhang Yu Tao Xingxing Zang Dajin Li Meirong Du | 2019 | Cellular & Molecular Immunology2019，16，12： | 23 |
| 20 | Whole-Genome Sequencing and Analysis of the Chinese Herbal Plant Panax notoginseng显示文摘 | Wei Chen Ling Kui Guanghui Zhang Shusheng Zhu Jing Zhang Xiao Wang Min Yang Huichuan Huang Yixiang Liu Yong Wang Yahe Li Lipin Zeng Wen Wang Xiahong He Yang Dong Shengchao Yang | 2017 | Molecular Plant2017，10，6： | 21 |
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