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| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 3 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 4 | Curcumin inhibits cellular cholesterol accumulation by regulating SREBP-1/caveolin-1 signaling pathway in vascular smooth muscle cells显示文摘目的: 在 anti-atherosclerosis.Methods 上调查保护的效果和杂种狗枯茗的可能的机制: 从 apoE 大美人拿的动脉粥样硬化患者损害的词法变化(apoE^(-/-))mice 被染色的 hematoxylin 曙红决定。细胞内部的类脂化合物微滴和类脂化合物层次是由油红O染色的 assayed , caveolin-1 的 HPLC.The 蛋白质表示被西方的 blotting.Translocation 和甾醇反应元素绑定 protein-1 ( SREBP-1 )的表示确定被免疫荧光 analysis.Results 间接地检测: 20 mg ·k g~的管理( -1)·d^(-1)curcumin 到apoE^(为 4 个月的 -/-)mice 导致了动脉粥样硬化患者损害的50%减小并且作为与模型组相比在 caveolin-1 表示水平产出5褶层增加。有 50 mg ·L ~的老鼠脉管的平滑肌房间( VSMC ) pretreated (为 48 h 的 -1)ox-lipid 密度脂蛋白( ox-LDL )增加了细胞的类脂化合物内容,并且刺激了 SREBP-1 易位,但是减少 装载level.Lipid 的房间在各种各样的集中暴露了到杂种狗枯茗的 caveolin-1 表示( 12.5,25 ,并且 50 亩摩尔 ·L ^( -1))for 不同持续时间( 0,6,12,24 ,和 48 h )显著地减少了细胞的类脂化合物微滴的数字和区域,全部的胆固醇,胆固醇酉旨,并且伴随 caveolin-1 表示的举起的免费胆固醇铺平(近似3褶层);到原子核的从细胞质的 SREBP-1 的易位与暴露于 N-acetyl-Leu-Leu-norleucinal 的 装载models.Lipid 的 VSMC 相比被禁止,一个 SREBP-1 朊酶禁止者, SREBP-1 的显示出的增加的原子易位,减少的 caveolin-1 表示水平,并且在调整细胞的类脂化合物 levels.Conclusion 上面:姜黄色素通过经由 SREBP-1 的原子易位的抑制增加 caveolin-1 表示在有教养的 VSMC 禁止 ox-LDL-induced 胆固醇累积。 | Hao-yu YUAN Shuang-yu KUAN Xing ZHENG Hong-yan LING Yun-Bo YANG Peng-Ke YAN Kai LI Duan-Fang LIAO | 2008 | Acta Pharmacologica Sinica2008，29，5： | 21 |
| 5 | Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer显示文摘渗入肿瘤的 Tregs 的起源,肿瘤免疫力的抑制的批评调停人,是不清楚的。这里,,我们证明在人的乳癌的渗入肿瘤的天真的 CD4 + T 房间和 Tregs 有重叠 TCR 全部剧目几乎别与传播 Tregs 重叠,建议 intratumoral Tregs 主要在 situ 而非从招募的 Tregs 从天真的 T 房间发展。而且,许多天真的 CD4 + T 房间和 Tregs 密切被相关,两个显示的穷人为乳癌病人的预后。天真的 CD4 + T 房间与许多生产 CCL18 巨噬细胞成比例遵守肿瘤片。而且,采纳地转移的人的天真的 CD4 + T 房间以一种 CCL18 依赖的方式渗入人的乳癌 orthotopic 异种皮移植。在在人性化的鼠标的人的乳癌异种皮移植,由将 PITPNM3 的表达式击倒堵住天真的 CD4 + T 房间的招募进肿瘤, CCL18 受体,显著地减少 intratumoral Tregs 并且禁止肿瘤前进。这些调查结果建议那个乳房渗入肿瘤的 Tregs 从在 situ 区分进 Tregs 的传播天真的 CD4 + T 房间的趋化性产生。由防碍 CCL18 的 PITPNM3 识别禁止天真的 CD4 + T 房间招募进肿瘤可以是为 anticancer 免疫疗法的吸引人的策略。 | Shicheng Su Jianyou Liao Jiang Liu Di Huang Chonghua He Fei Chen LinBing Yang Wei Wu Jianing Chen Ling Lin Yunjie Zeng Nengtai Ouyang Xiuying Cui Herui Yao Fengxi Su Jian-dong Huang Judy Lieberman Qiang Liu Erwei Song | 2017 | Cell Research2017，27，4： | 19 |
| 6 | Citrate versus unfractionated heparin for anticoagulation in continuous renal replacement therapy显示文摘 | LIAO Yu-jie ZHANG Ling ZENG Xiao-xi FU Ping | 2013 | Chinese Medical Journal2013，，7： | 18 |
| 7 | Dihydromyricetin improves type 2 diabetes- induced cognitive impairment via suppressing oxidative stress and enhancing brain-derived neurotrophic factor-mediated neuroprotection in mice显示文摘类型 2 糖尿病 mellitus (T2DM ) 导致认知缺陷(CI ) ,但是为在 T2DM 的认知机能障碍没有有效 pharmacotherapies 或药。Dihydromyricetin (DHM ) 是从白蔹属 grossedentata 的叶子提取的自然 flavonoid 混合物并且包括抗氧化剂和反糖尿病有各种各样的药理学效果。因此,我们在 T2DM 老鼠模型和它的可能的机制在 CI 上调查了 DHM 的效果。导致 T2DM,老鼠用高糖、高脂肪的饮食被喂 8 个星期,由低剂量 streptozotocin (STZ ) 列在后面管理。在 T2DM 鼠标模型的成功的正式就职以后,鼠标与相等的体积分别地被对待盐(T2DM 组) , 125 mg/kg/d DHM (L-DHM 组) ,或 250 mg/kg/d DHM (H-DHM 组) 。在 16 星期 DHM 管理以后,身体重量(BW ) , fasting 血葡萄糖,血类脂化合物, intraperitoneal 葡萄糖忍耐(IPGT ) ,和认知功能是坚定的。然后,在在马头鱼尾的怪兽的氧化压力标记和导出大脑的神经营养的因素(BDNF ) 的表情的改变被调查。我们的调查结果证明 DHM 能显著地改善在 T2DM 老鼠的 CI 和反向的异常葡萄糖和类脂化合物新陈代谢,通过调停 BDNF 的 neuroprotection 的氧化应力和改进的抑制可能。在结论,我们的结果建议 DHM 是导致 T2DM 的认知机能障碍的处理的一个有希望的候选人。 | Hongyan Ling Zemei Zhu Jihua Yang Jianqin He Sisi Yang Di Wu Shuidong Feng Duanfang Liao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，3： | 14 |
| 8 | Ciliary parathyroid hormone signaling activates transforming growth factor-βto maintain intervertebral disc homeostasis during aging显示文摘Degenerative disc disease(DDD) is associated with intervertebral disc degeneration of spinal instability. Here, we report that the cilia of nucleus pulposus(NP) cells mediate mechanotransduction to maintain anabolic activity in the discs. We found that mechanical stress promotes transport of parathyroid hormone 1 receptor(PTH1 R) to the cilia and enhances parathyroid hormone(PTH) signaling in NP cells. PTH induces transcription of integrin α_vβ_6 to activate the transforming growth factor(TGF)-β-connective tissue growth factor(CCN2)-matrix proteins signaling cascade. Intermittent injection of PTH(iPTH) effectively attenuates disc degeneration of aged mice by direct signaling through NP cells, specifically improving intervertebral disc height and volume by increasing levels of TGF-β activity, CCN2, and aggrecan. PTH1 R is expressed in both mouse and human NP cells. Importantly,knockout PTH1 R or cilia in the NP cells results in significant disc degeneration and blunts the effect of PTH on attenuation of aged discs. Thus, mechanical stress-induced transport of PTH1 R to the cilia enhances PTH signaling, which helps maintain intervertebral disc homeostasis, particularly during aging, indicating therapeutic potential of iPTH for DDD. | Liwei Zheng Yong Cao Shuangfei Ni Huabin Qi Zemin Ling Xin Xu Xuenong Zou Tianding Wu Ruoxian Deng Bo Hu Bo Gao Hao Chen Yusheng Li Jianxi Zhu Francis Tintani Shadpour Demehri Amit Jain Khaled M.Kebaish Shenghui Liao Cheryle A.Séguin Janet L.Crane Mei Wan Hongbin Lu Paul D.Sponseller Lee H.RileyIII Xuedong Zhou Jianzhong Hu Xu Cao | 2018 | Bone Research2018，6，3： | 13 |
| 9 | Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia显示文摘Objective To investigate the association between total homocysteine(tHcy)level in plasma and methylenetetrahydrofblate reductase(MTHFR)C677T and A1298C genetic polymorphisms in a Chinese Han nationality population with type 2 diabetes mellitus(T2DM)accompanied by dyslipidemia.Methods This case-control study enrolled T2DM patients with dyslipidemia and without dyslipidemia respectively.Sanger dideoxy-mediated chain-termination method was used to detect the gene polymorphisms of MTHFR C677T and A1298C.Plasma tHcy and lipid levels were measured as well.The genotype frequency and allele frequency between the dyslipidemia and non-dyslipidemia groups were compared by using Chi-square test.Plasma tHcy level ofT2DM patients who carried the different genotypes was compared by Student's t test.Results Finally,82 T2DM patients with dyslipidemia and 94 ones without dyslipidemia were included in this study.There was a significant correlation between tHcy level and MTHFR C677T gene polymorphism inT2DM patients(t=2.27,P=0.02).Moreover,the plasma tHcy level in the dyslipidemia patients who carried MTHFR 677TT genotype was significantly higher than that in those with CT+CC genotype(13.62+6.97 vs.10.95+3.62pmol/L,t=2.2O,P=0.03);while for patients without dyslipidemia,comparison of the tHcy level between those who carried the above two alleles showed no significantly difference(13.34±6.03 vs.12.04±5.09μmol/L,t=1.08,P=0.29).Conclusion MTHFR 677TT genotype might associate with higher tHcy level in T2DM patients with dyslipidemia. | Ying Yin Rui Li Xiaoli Li Kunrong Wu Ling Li Yuedong Xu Lin Liao Rui Yang Yan Li | 2020 | Chinese Medical Sciences Journal2020，35，1： | 10 |
| 10 | Effect of Heat Treatment on Cu Distribution,Antibacterial Performance and Cytotoxicity of Ti-6Al-4V-5Cu Alloy显示文摘A copper-bearing Ti-6AI-4V-5Cu alloy was processed and subjected to different heat treatments to explore the relationship among microstructure,antibacterial performance,and cytocompatibility.Characterization of microstructure revealed that the solution treated alloy consisted of α phase,α' phase and(3 phase,while besides these phases,the aged alloy also contained the precipitations of intermetallic Ti_2Cu compound.The solution treated alloy showed better antibacterial performance with increasing the solution temperature.The Cu ions released from Ti—6AI—4V—5Cu alloy could effectively inhibit the formation of bacterial biofilm on the surface of alloy,and do not induce any cytotoxicity.The optimal heat treatment for Ti-6Al-4V-5Cu alloy was solution treated at 930 C,at which it could exhibit both promising antibacterial performance and no cytotoxicity. | Zheng Ma Ling Ren Rui Liu Ke Yang Yu Zhang Zhenhua Liao Weiqiang Liu Min Qi R.D.K.Misra | 2015 | Journal of Materials Science & Technology2015，31，7： | 10 |
| 11 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
| 12 | Monoterpene Glycosides from the Roots of Paeonia lactiflora显示文摘A new monoterpene glycoside,together with nine known ones,3-O-methylpaeoniflorin,mudanpioside J,paeoniflorin,benzoylpaeoniflorin,oxypaeoniflorin,benzoyloxypaeoniflorin,oxybenzoylpaeoniflorin,albiflorin and lactiflorin,was isolated from the roots of Paeonia lactiflora Pall.. The structure of the new compound was elucidated as galloylalbiflorin by the spectro-scopic evidence including ESI-MS,1D-and 2D-NMR spectra. | Xiao Ling WANG Wei JIAO Xun LIAO Shu Ling PENG Li Sheng DING | 2006 | Chinese Chemical Letters2006，17，7： | 7 |
| 13 | Association of urinary albumin excretion with central foveal thickness and intravitreal conbercept treatment frequency in patients with diabetic macular edema显示文摘AIM: To investigate the effect of albuminuria on diabetic macular edema(DME) and the possible association between baseline urinary albumin excretion(UAE) and intravitreal conbercept(IVC) treatment frequency in DME patients. METHODS: In this hospital-based retrospective study, a total of 350 in-patients with type 2 diabetes mellitus were recruited and their clinical records were reviewed. Thereafter, 52 patients identified with severe non-proliferative diabetic retinopathy(NPDR) combined with albuminuria were divided into the microalbuminuria(UAE 30-300 mg/24 h) and macroalbuminuria(UAE>300 mg/24 h) groups, which were compared and analyzed by both independent sample t-test and Chi-square test. Correlations between the systemic variables and the central foveal thickness(CFT) were evaluated using Spearman’s correlation and linear regression analyses. Of the 52 patients with centerinvolved DME, 43 received an initial combined injection of conbercept(0.5 mg/0.05 mL) and triamcinolone acetonide(1 mg/0.05 mL), followed by an IVC injection, as needed. The relationship between baseline UAE and number of IVCinjections during the first year of treatment was analyzed using Spearman’s partial correlation.RESULTS: Of 350 patients, a higher incidence of DME was observed in severe non-proliferative retinopathy(NPDR) patients than that observed in other groups. By dividing the 52 patients with severe NPDR into the micro-and macro-albuminuria subgroups, significant differences in CFT, systolic blood pressure, total cholesterol and serum creatinine levels, and UAE were revealed. Furthermore, a positive liner correlation between the UAE and CFT was found. Finally, the partial correlation coefficient adjusted for either the CFT or UAE indicated that both parameters directly correlated with the number of IVC injections administered during the 12 mo of follow-up. CONCLUSION: Generally, macular edema occurred in patients with severe NPDR, for whom the UAE is an independent risk predictor of DME. The baseline UAE and CFT predicted the treatment frequency of IVC injections administered in the first year for eyes with DME. | Zi-Yao Liu Xiao-Jie Ma Ding-Ying Liao Xin-Di Liu Ling Bai Jing Yao Min Xu Yu-Ping Zheng | 2019 | International Journal of Ophthalmology(English edition)2019，12，10： | 6 |
| 14 | Coagulation factors VII, IX and X are effective antibacterial proteins against drug-resistant Gram-negative bacteria显示文摘Infections caused by drug-resistant“superbugs”pose an urgent public health threat due to the lack of effective drugs;however, certain mammalian proteins with intrinsic antibacterial activity might be underappreciated. Here, we reveal an antibacterial property against Gram-negative bacteria for factors VII, IX and X, three proteins with well-established roles in initiation of the coagulation cascade. These factors exert antibacterial function via their light chains (LCs). Unlike many antibacterial agents that target cell metabolism or the cytoplasmic membrane, the LCs act by hydrolyzing the major components of bacterial outer membrane, lipopolysaccharides, which are crucial for the survival of Gram-negative bacteria. The LC of factor VII exhibits in vitro efficacy towards all Gram-negative bacteria tested, including extensively drug-resistant (XDR) pathogens, at nanomolar concentrations. It is also highly effective in combating XDR Pseudomonas aeruginosa and Acinetobacter baumannii infections in vivo. Through decoding a unique mechanism whereby factors VII, IX and X behave as antimicrobial proteins, this study advances our understanding of the coagulation system in host defense, and suggests that these factors may participate in the pathogenesis of coagulation disorder-related diseases such as sepsis via their dual functions in blood coagulation and resistance to infection. Furthermore, this study may offer new strategies for combating Gram-negative “superbugs”. | Jinwu Chen Xiaojie Li Ling Li Ting Zhang Qing Zhang Fangming Wu Diyue Wang Hongze Hu Changlin Tian Dongsheng Liao Liang Zhao Danxia Song Yongyun Zhao Chuanfang Wu Xu Song | 2019 | Cell Research2019，29，9： | 6 |
| 15 | Hypoxia inducible factor-1alpha mediates protection of DL-3-n-butylphthalide in brain microvascular endothelial cells against oxygen glucose deprivation-induced injury显示文摘Studies have demonstrated that DL-3-n-butylphthalide can significantly alleviate oxygen glucose deprivation-induced injury of human umbilical vein endothelial cells at least partly associated with its enhancement on oxygen glucose deprivation-induced hypoxia inducible factor-1α expression.In this study,we hypothesized that DL-3-n-butylphthalide can protect against oxygen glucose deprivation-induced injury of newborn rat brain microvascular endothelial cells by means of upregulating hypoxia inducible factor-1α expression.MTT assay and Hoechst staining results showed that DL-3-n-butylphthalide protected brain microvascular endothelial cells against oxygen glucose deprivation-induced injury in a dose-dependent manner.Western blot and immunofluorescent staining results further confirmed that the protective effect was related to upregulation of hypoxia inducible factor-1α.Real-time RT-PCR reaction results showed that DL-3-n-butylphthalide reduced apoptosis by inhibiting downregulation of pro-apoptotic gene caspase-3 mRNA expression and upregulation of apoptosis-executive protease bcl-2 mRNA expression;however,DL-3-n-butylphthalide had no protective effects on brain microvascular endothelial cells after knockdown of hypoxia inducible factor-1α by small interfering RNA.These findings suggest that DL-3-n-butylphthalide can protect brain microvascular endothelial cells against oxygen glucose deprivation-induced injury by upregulating bcl-2 expression and downregulating caspase-3 expression though hypoxia inducible factor-1α pathway. | Weihong Yang Ling Li Ruxun Huang Zhong Pei Songjie Liao Jinsheng Zeng | 2012 | Neural Regeneration Research2012，7，12： | 6 |
| 16 | Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects显示文摘β-Catenin plays a critical role in cartilage formation and development. To further understand the role of β-catenin in osteoarthritis(OA) development in temporomandibular joint(TMJ), we have generated β-catenin conditional activation mice(β-cat(ex3)^(Agc1CreER))by breeding Agc1-CreER mice with β-catenin^(flox(ex3)/+)mice. Results of histologic analysis showed the progressive TMJ defects in 3-and 6-month-old β-cat(ex3)^(Agc1CreER)mice(tamoxifen induction was performed at 2 weeks of age), including decreased chondrocyte numbers in the superficial layer associated with less Alcian blue staining, increased numbers of hypertrophic chondrocytes in deep layers, and rough articular surface. Compared to the TMJ phenotype of β-cat(ex3)^(Col2CreER)mice, β-cat(ex3)^(Agc1CreER)mice showed much severe morphological defects in the superficial layer of TMJ. This may reflect that Agc1-CreER mice could efficiently target cells in the superficial layer of TMJ. Results of immunostaining showed significantly increased expression of MMP13, Col-X, Adamts4,and Adamts5 in TMJ of β-cat(ex3)^(Agc1CreER)mice. Results of proliferating cell nuclear antigen(PCNA), Ki67, and terminal deoxinucleotidyl transferase-mediated d UTP-fluorescein nick end labeling(TUNEL) staining further demonstrated that cell proliferation was decreased and cell apoptosis was increased in condylar cartilage of β-cat(ex3)^(Agc1CreER)mice. Our findings indicate that abnormal upregulation of β-catenin in TMJ leads to defects assembling to OA-like phenotype, further demonstrating that β-catenin plays a critical role in TMJ pathogenesis. | Tianqian Hui Yachuan Zhou Tingyu Wang Jun Li Shanxing Zhang Lifan Liao Jianhong Gu Ling Ye Lan Zhao Di Chen | 2018 | International Journal of Oral Science2018，10，2： | 6 |
| 17 | Antibacterial Properties of Ti-6Al-4V-xCu Alloys显示文摘Surgical implant-associated bacterial infection is becoming a serious clinical problem.A series of copper-bearing titanium alloy,Ti—6AI—4V—xCu(x = 1,3,5 wt%),were fabricated in the present study in order to reduce the hazard of the bacterial infection problem by means of the strong antibacterial ability of Cu element.The metallography,X-ray diffraction,antibacterial ability,corrosion resistance and cytotoxicity of Ti—6AI—4V—xCu alloys were preliminarily studied with comparison to the commercial medical Ti—6AI—4V alloy.The Ti—6AI—4V—xCu alloys showed obvious antibacterial abilities with good corrosion resistance and cytocompatibility,and the antibacterial role was enhanced with increasing Cu content,which has significant potential for clinical applications as surgical implant materials. | Ling Ren Zheng Ma Mei Li Yu Zhang Weiqiang Liu Zhenhua Liao Ke Yang | 2014 | Journal of Materials Science & Technology2014，30，7： | 5 |
| 18 | Formulation of dried lignans nanosuspension with high redispersibility to enhance stability,dissolution,and oral bioavailability显示文摘Herpetospermum caudigerum lignans(HTL), one of the potential drugs with anti-hepatitis B virus and hepatoprotective effects, has limited clinical applications because of poor aqueous solubility and low bioavailability. Both herpetrione(HPE) and herpetin(HPN) are the most abundant ingredients in HTL and exhibit weak acidity. The purpose of the present study was to produce dried preparations of HTL(composed of HPE and HPN) nanosuspensions(HTL-NS) with high redispersibility using lyophilization technology. The HTL-NS was prepared by utilizing precipitation-combined homogenization technology based on acid-base neutralization reactions, and critical formulation and process parameters affecting the characteristics of HTL-NS were optimized. The resultant products were characterized by particle size analysis, SEM, XRD, stability, solubility, dissolution and in vivo bioavailability. HTL-NS showed near-spherical-shaped morphology and the size was 243 nm with a narrow PDI value of 0.187. The dried preparations with a relatively large particle size of 286 nm and a PDI of 0.215 were achieved by using 4%(W/V) mannitol as cryoprotectants, and had a better stability at 4 or 25 oC for 2 months, compared to HTL-NS. In the in vitro test, the dried preparations showed markedly increased solubility and dissolution velocity. Besides, in the in vivo evaluation, it exhibited significant increases in AUC0–t, C_(max), MRT and a decrease in T_(max), compared to the raw drug.. In conclusion, our results provide a basis for the development of a drug delivery system for poorly water-soluble ingredients with p H-dependent solubility. | SHEN Gang CHENG Ling WANG Li-Qiang ZHANG Li-Hong SHEN Bao-De LIAO Wei-Bo LI Juan-Juan ZHENG Juan XU Rong YUAN Hai-Long | 2016 | Chinese Journal of Natural Medicines2016，14，10： | 5 |
| 19 | Angiopoletin-1 protects myocardial endothelial cell function blunted by angiopoietin-2 and high glucose condition显示文摘 | Qin-hui TUO Guo-zuo XIONG Heng ZENG Hei-di YU Shao-wei SUN Hong-yan LING Bing-yang ZHU Duan-fang LIAO Jian-xiong CHEN | 2011 | Acta Pharmacologica Sinica2011，32，1： | 4 |
| 20 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
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