| 1 | 测定卵裂球X染色体连锁基因剂量鉴别小鼠胚胎性别显示文摘将8-细胞小鼠胚胎除丢透明带,从中取出一个卵裂球,然后测定其与X染色体连锁的次黄嘌呤磷酸核糖基转移酶(Hypoxanthinephosphoribosyl transferase,HPRT)和与常染色体连锁的腺嘌呤磷酸核糖基转移酶(Adenine phosphoribosyl transferase,APRT)的活性,由于在8-细胞阶段,雌性胚胎中的两条X染色体均有活性,因此,可根据HPRT活性在基因剂量上的二倍差异,得到HPRT与APRT比率的双态分布图,而鉴别出雄性和雌性胚胎(?)将几批用这种方法鉴别了性别的胚胎移植到假孕受体小鼠子宫内。结果,其中八分之七胚胎的发育与对照组去了透明带的完整胚胎的发育相一致。通过测是X连锁基因剂量鉴别胚胎性别不仅准确,而且快速,要鉴别性别的胚胎移植前不需低温保存等待鉴别结果。 | M.Monk 黄国清 | 1989 | 国外畜牧科技1989，16，6： | 2 |
| 2 | Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets显示文摘In silico genome mining provides easy access to secondary metabolite biosynthetic gene clusters(BGCs)encoding the biosynthesis of many bioactive compounds,which are the basis for many important drugs used in human medicine.However,the association between BGCs and other functions encoded in the genomes of producers have remained elusive.Here,we present a systems biology workflow that integrates genome mining with a detailed pangenome analysis for detecting genes associated with a particular BGC.We analyzed 3,889 enterobacterial genomes and found 13,266 BGCs,represented by 252 distinct BGC families and 347 additional singletons.A pangenome analysis revealed 88 genes putatively associated with a specific BGC coding for the colon cancer-related colibactin that code for diverse metabolic and regulatory functions.The presented workflow opens up the possibility to discover novel secondary metabolites,better understand their physiological roles,and provides a guide to identify and analyze BGC associated gene sets. | Omkar S.Mohite Colton J.Lloyd Jonathan M.Monk Tilmann Weber Bernhard O.Palsson | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 0 |
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