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| 1 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 2 | Design and experiment on intelligent fuzzy monitoring system for corn planters显示文摘When sowing summer corn without tillage,it is necessary to ensure that the furrow opener is free from straw congestion and that the spacing of the sowing can be adjusted according to the breeds of corn and the preset seeding rate per acre.On the basis of the structural features of newly developed no-tillage corn fertilizers,an intelligent fuzzy monitoring system for corn planters was developed in this study.The system facilitates automatic control of the spacing adjustment and the status monitor for the fertilizer tank,seed tank,and seeding orifice.According to the preset number of rows,line spacing,number of plants per acre,and seed germination rate,the control rate can be calculated through designing in surveillance software.The control rate is output to the fuzzy controller through the digital output module of the CAN bus.Fuzzy control is applied to the DC motor for stepless adjustment of the spacing.A system for video surveillance of the working status of a planter is developed for displaying a real-time video image of the planter operation and achieving an anti-congestion status monitoring of a no-tillage planting operation in a dusty environment.Through field trials,the detection accuracy was 91.4%.The seed-clogging fault-alarm accuracy was 96.0%.The entire system remained stable and reliable. | Du Ruicheng Gong Bingcai Liu Ningning Wang Chenchen Yang Zidong Ma Mingjian | 2013 | International Journal of Agricultural and Biological Engineering2013，6，3： | 16 |
| 3 | Nucleic acids and analogs for bone regeneration显示文摘With the incidence of different bone diseases increasing, effective therapies are needed that coordinate a combination of various technologies and biological materials. Bone tissue engineering has also been considered as a promising strategy to repair various bone defects. Therefore, different biological materials that can promote stem cell proliferation, migration, and osteoblastic differentiation to accelerate bone tissue regeneration and repair have also become the focus of research in multiple fields. Stem cell therapy, biomaterial scaffolds, and biological growth factors have shown potential for bone tissue engineering; however, off-target effects and cytotoxicity have limited their clinical use. The application of nucleic acids(deoxyribonucleic acid or ribonucleic acid)and nucleic acid analogs(peptide nucleic acids or locked nucleic acids), which are designed based on foreign genes or with special structures, can be taken up by target cells to exert different effects such as modulating protein expression, replacing a missing gene, or targeting specific gens or proteins. Due to some drawbacks, nucleic acids and nucleic acid analogs are combined with various delivery systems to exert enhanced effects, but current studies of these molecules have not yet satisfied clinical requirements. In-depth studies of nucleic acid or nucleic acid analog delivery systems have been performed, with a particular focus on bone tissue regeneration and repair. In this review, we mainly introduce delivery systems for nucleic acids and nucleic acid analogs and their applications in bone repair and regeneration. At the same time, the application of conventional scaffold materials for the delivery of nucleic acids and nucleic acid analogs is also discussed. | Yuxin Zhang Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Xueping Xie Xiawei Wei Yunfeng Lin | 2018 | Bone Research2018，6，4： | 3 |
| 4 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 5 | Multi?targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence显示文摘Biofilm formation is responsible for numerous chronic infections and represents a serious health challenge.Bacteria and the extracellular polysaccharides(EPS)cause biofilms to become adherent,toxic,resistant to antibiotics,and ultimately difficult to remove.Inhibition of EPS synthesis can prevent the formation of bacterial biofilms,reduce their robustness,and promote removal.Here,we have developed a framework nucleic acid delivery system with a tetrahedral configuration.It can easily access bacterial cells and functions by delivering antisense oligonucleotides that target specific genes.We designed antisense oligonucleotide sequences with multiple targets based on conserved regions of the VicK protein-binding site.Once delivered to bacterial cells,they significantly decreased EPS synthesis and biofilm thickness.Compared to existing approaches,this system is highly efficacious because it simultaneously reduces the expression of all targeted genes(gtfBCD,gbpB,ftf).We demonstrate a novel nucleic acid-based nanomaterial with multi-targeted inhibition that has great potential for the treatment of chronic infections caused by biofilms. | Yuxin Zhang Xueping Xie Wenjuan Ma Yuxi Zhan Chenchen Mao Xiaoru Shao Yunfeng Lin | 2020 | Nano-Micro Letters2020，12，6： | 3 |
| 6 | The biological applications of DNA nanomaterials:current challenges and future directions显示文摘DNA,a genetic material,has been employed in different scientific directions for various biological applications as driven by DNA nanotechnology in the past decades,including tissue regeneration,disease prevention,inflammation inhibition,bioimaging,biosensing,diagnosis,antitumor drug delivery,and therapeutics.With the rapid progress in DNA nanotechnology,multitudinous DNA nanomaterials have been designed with different shape and size based on the classic Watson–Crick base-pairing for molecular self-assembly.Some DNA materials could functionally change cell biological behaviors,such as cell migration,cell proliferation,cell differentiation,autophagy,and anti-inflammatory effects.Some single-stranded DNAs(ssDNAs)or RNAs with secondary structures via self-pairing,named aptamer,possess the ability of targeting,which are selected by systematic evolution of ligands by exponential enrichment(SELEX)and applied for tumor targeted diagnosis and treatment.Some DNA nanomaterials with three-dimensional(3D)nanostructures and stable structures are investigated as drug carrier systems to delivery multiple antitumor medicine or gene therapeutic agents.While the functional DNA nanostructures have promoted the development of the DNA nanotechnology with innovative designs and preparation strategies,and also proved with great potential in the biological and medical use,there is still a long way to go for the eventual application of DNA materials in real life.Here in this review,we conducted a comprehensive survey of the structural development history of various DNA nanomaterials,introduced the principles of different DNA nanomaterials,summarized their biological applications in different fields,and discussed the current challenges and further directions that could help to achieve their applications in the future. | Wenjuan Ma Yuxi Zhan Yuxin Zhang Chenchen Mao Xueping Xie Yunfeng Lin | 2021 | Signal Transduction and Targeted Therapy2021，6，11： | 2 |
| 7 | Challenges and opportunities for managing pediatric central nervous system tumors in China显示文摘Central nervous system(CNS)tumors represent the most deadly cancer in pediatric age group.In China,thousands of children are diagnosed with CNS tumors every year.Despite the improving socioeconomic status and availability of medical expertise within the country,unique challenges remain for the delivery of pediatric neuro-oncology service.In this review,we discuss the existing hurdles for improving the outcome of children with CNS tumors in China.Need for precise disease burden estimation,lack of intra-and inter-hospital collaborative networks,high probability of treatment abandonment,along with financial toxicities from treatment represent the key challenges that Chinese healthcare providers encounter.The tremendous opportunities for advancing the status of pediatric neuro-oncology care in and beyond the country are explored. | Anthony Pak-Yin Liu Daniel CMoreira Chenchen Sun Lisa Krull Yijin Gao Bo Yang Chenran Zhang Kejun He Xiaojun Yuan Godfrey Chi-Fung Chan Xiaofei Sun Xiaoli Ma Ibrahim AQaddoumi | 2020 | Pediatric Investigation2020，4，3： | 2 |
| 8 | How aerosol direct effects influence the source contributions to PM2.5 concentrations over Southern Hebei, China in severe winter haze episodes显示文摘 | Litao Wang Joshua S. Fu Wei Wei Zhe Wei Chenchen Meng Simeng Ma Jiandong Wang | 2018 | Frontiers of Environmental Science & Engineering2018，12，3： | 2 |
| 9 | CDKN2A/p16 Inactivation Mechanisms and Their Relationship to Smoke Exposure and Molecular Features in Non–Small-Cell Lung Cancer显示文摘 | Kit W. Tam Wei Zhang Junichi Soh Victor Stastny Min Chen Han Sun Kelsie Thu Jonathan J. Rios Chenchen Yang Crystal N. Marconett Suhaida. A. Selamat Ite A. Laird-Offringa Ayumu Taguchi Samir Hanash David Shames Xiaotu Ma Michael Q. Zhang Wan L. Lam Adi Gaz | 2013 | Journal of Thoracic Oncology2013，，11： | 1 |
| 10 | Phthalimide and Naphthalimide end-Capped Diketopyrrolopyrrole for Organic Photovoltaic Applications显示文摘二个小分子作为终端作为中央强壮的领受人单位和 phthalimide/naphthalimide 与一个 DPP 核心说出 PI-DPP 和 NI-DPP 弱领受人被设计并且综合。热行为,光、电气化学的性质,以及这二材料的光电的表演上的终端 phthalimide/naphthalimide 单位的效果系统地被学习。周期的 voltammetry 表明两个分子的最低没有住的分子的 orbitals (LUMO )(^-3.6 eV ) 对普通电子施主(P3HT ) 和领受人(PCBM ) 中间。当与 PCBM 相配时,当与 P3HT 相配时,在在 DPP 和 PCBM 之间,以及在 P3HT 和 DPP 之间的足够的驱动力应该为激子分离被创造的地方,这显示 PI-DPP 和 NI-DPP 可以特别地用作电子施主,并且作为电子领受人。用作电子施主材料, PI-DPP 和 NI-DPP 设备由分别地与 PCBM 相配展出了 0.90% 和 0.76% 的低力量变换效率(PCE ) 。并且 NI-DPP 的潜力的初步的评估作为施主材料,和 P3HT 用 P3HT 作为电子领受人材料被执行: NI-DPP 设备显示出0.6%的 PCE ,与开的电路电压( V 0.7 V 的 OC ),短路电流密度( J 1.91 mAcm -2,和45%的一个fill 因素( FF )的 SC )。 | Ming Chen Chenchen Du Xiaolei Ren Maoheng Yi Jinduo Yi Chufeng Chen Feng Liu Minjie Li Changqi Ma Hongyu Wang | 2017 | Chinese Journal of Chemistry2017，35，9： | 1 |
| 11 | Construction of Porcine Growth Hormone Eukaryotic Expression Vector and Its Transfection Mediated by Cationic Liposome in Mice显示文摘 | Chenchen Peng Lihong Wang Zizhu Chen Lei Ma Yan Wei Zhangfu Long | 2011 | Animal Biotechnology2011，，4： | 1 |
| 12 | Inhibition of autophagy by 3?MA enhances endoplasmic reticulum stress?inducedapoptosis in human nasopharyngeal carcinoma cells显示文摘 | Lele Song Hao Liu Linyan Ma Xudng Zhang Zhiwen Jiang Chenchen Jiang | 2013 | Oncology Letters2013，，4： | 1 |
| 13 | Generation and application of replication-competent Venus-expressing H5N1,H7N9,and H9N2 influenza A viruses显示文摘The generation and application of replication-competent influenza A virus(IAV) expressing a reporter gene represent a valuable tool to elucidate the mechanism of viral pathogenesis and establish new countermeasures to combat the threat of influenza. Here, replication-competent IAVs with a neuraminidase(NA) segment harboring a fluorescent reporter protein, Venus, were generated in the background of H5N1, H7N9, and H9N2 influenza viruses, the three subtypes of viruses with imminent pandemic potential. All three reporter viruses maintained virion morphology, replicated with similar or slightly reduced titers relative to their parental viruses, and stably expressed the fluorescent signal for at least two passages in embryonated chicken eggs. As a proof of concept, we demonstrated that these reporter viruses,used in combination with a high-content imaging system, can serve as a convenient and rapid tool for the screening of antivirals and host factors involved in the virus life cycle. Moreover, the reporter viruses demonstrated similar growth properties and tissue tropism as their parental viruses in mice, among which the H7N9 NA-Venus virus could potentially be used in ex vivo studies to better understand H7N9 pathogenesis or to develop novel therapeutics. | guangwen wang jie zhang fandi kong qibing li jinliang wang shujie ma yuhui zhao libin liang junping li nan sun lizheng guan yuan zhou chenchen zhou shanyu huang zhigao bu li jiang hualan chen chengjun li | 2018 | Science Bulletin2018，63，3： | 1 |
| 14 | Bio-template Synthesis of Spirulina/a-Fe2O3 Composite with Improved Surface Wettability显示文摘 | MENG Qingling XIE Chenchen DING Ran CAO Liang MA Ke LI Li WENG Zhankun WANG Zuobin | 2018 | Chemical Research in Chinese Universities2018，34，6： | 0 |
| 15 | NLRP3 inflammasome activation mechanism and its role in autoimmune liver disease显示文摘The NLRP3 inflammasome is a multiprotein binding compound comprising NLRP3,connector protein ASC,and effector protein pro-caspase-1.When the NLRP3 inflammasome senses a danger signal from the host or pathogen,activated caspase-1 cleaves the precursors of interleukin(IL)-1βand IL-18 into mature proinflammatory cytokines,simultaneously causing lysis via the pore-forming protein gasdermin D.This induction of cell inflammatory pyroptosis suggests that it is a key process in the innate immune response to pathogens or cellular stress.Recent studies have shown that NLRP3 inflammasome also plays an important role in regulating autoimmune liver diseases,including autoimmune hepatitis,primary biliary cholangitis,and primary sclerosclerotic cholangitis.In this review,we summarize the structure,activation and modulation of the NLRP3 inflammasome,highlight the progress in research on the role of NLRP3 inflammasome in the occurrence and development of autoimmune liver diseases,and discuss potential strategies for targeting the NLRP3 inflammasome in the treatment of autoimmune liver diseases. | Yanling Guan Yiyue Gu Hao Li Bo Liang Chenchen Han Yu Zhang Qian Liu Wei Wei Yang Ma | 2022 | Acta Biochimica et Biophysica Sinica2022，54，11： | 0 |
| 16 | Advances in CIGS thin film solar cells with emphasis on the alkali element post-deposition treatment显示文摘In the past tens of years,the power conversion efficiency of Cu(In,Ga)Se2(CIGS)has continuously improved and been one of the fastest growing photovoltaic technologies that can also help us achieve the goal of carbon emissions reduction.Among several key advances,the alkali element post-deposition treatment(AlK PDT)is regarded as the most important finding in the last 10 years,which has led to the improvement of CIGS solar cell efficiency from 20.4%to 23.35%.A profound understanding of the influence of alkali element on the chemical and electrical properties of the CIGS absorber along with the underlying mechanisms is of great importance.In this review,we summarize the strategies of the alkali element doping in CIGS solar cell,the problems to be noted in the PDT process,the effects on the CdS buffer layer,the effects of different alkali elements on the structure and morphology of the CIGS absorber layer,and retrospect the progress in the CIGS solar cell with emphasis on the alkali element post deposition treatment. | Chenchen Zhao Shen Yu Wei Tang Xinye Yuan Hongfei Zhou Tongqing Qi Xue Zheng De Ning Ming Ma Junyi Zhu Jie Zhang Chunlei Yang Weimin Li | 2023 | Materials Reports(Energy)2023，3，3： | 0 |
| 17 | Molecular confined synthesis of magnetic CoO_(x)/Co/C hybrid catalyst for photocatalytic water oxidation and CO_(2)reduction显示文摘Photosynthesis[6CO_(2)+12H_(2)O→(CH_(2)O)+6O_(2)+6H_(2)O]in nature contains a light reaction process for oxygen evolution and a dark reaction process for carbon dioxide(CO_(2))reduction to carbohydrates,which is of great significance for the survival of living matter.Therefore,for simulating photosynthesis,it is desirable to design and fabricate a bifunctional catalyst for promoting photocatalytic water oxidation and CO_(2)reduction performances.Herein,a molecular confined synthesis strategy is reasonably proposed and applied,that is the bifunctional CoO_(x)/Co/C-T(T=700,800 and 900℃)photocatalysts prepared by the pyrolysis of molecular Co-EDTA under N_(2) and air atmosphere in turn.Among the prepared photocatalysts,the CoOx/Co/C-800 shows the best photocatalytic water oxidation activity with an oxygen yield of 51.2%.In addition,for CO_(2)reduction reaction,the CO evolution rate of 12.6μmol/h and selectivity of 75%can be achieved over this catalyst.The improved photocatalytic activities are attributed to the rapid electron transfer between the photosensitizer and the catalyst,which is strongly supported by the current densityvoltage G-V,steady-state and time-resolved photoluminescence spectra(PL).Overall,this work provides a reference for the preparation and optimization of photocatalysts with the capacity for water oxidation and CO_(2)reduction reactions. | Rui Li Chenchen Zhang Kejia You Bonan Li Wei Bu Xiangyu Meng Baochun Ma Yong Ding | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
| 18 | RFCNet:Remote Sensing Image Super-Resolution Using Residual Feature Calibration Network显示文摘In the field of single remote sensing image Super-Resolution(SR),deep Convolutional Neural Networks(CNNs)have achieved top performance.To further enhance convolutional module performance in processing remote sensing images,we construct an efficient residual feature calibration block to generate expressive features.After harvesting residual features,we first divide them into two parts along the channel dimension.One part flows to the Self-Calibrated Convolution(SCC)to be further refined,and the other part is rescaled by the proposed Two-Path Channel Attention(TPCA)mechanism.SCC corrects local features according to their expressions under the deep receptive field,so that the features can be refined without increasing the number of calculations.The proposed TPCA uses the means and variances of feature maps to obtain accurate channel attention vectors.Moreover,a region-level nonlocal operation is introduced to capture long-distance spatial contextual information by exploring pixel dependencies at the region level.Extensive experiments demonstrate that the proposed residual feature calibration network is superior to other SR methods in terms of quantitative metrics and visual quality. | Yuan Xue Liangliang Li Zheyuan Wang Chenchen Jiang Minqin Liu Jiawen Wang Kaipeng Sun Hongbing Ma | 2023 | Tsinghua Science and Technology2023，28，3： | 0 |
| 19 | Change Features of GPS/MET Precipitable Water Vapor in Different Precipitation in Lianyungang显示文摘Based on the GPS/MET water vapor monitoring data and conventional meteorological data at Lianyungang station from April to July,2015,the relationship between precipitable water vapor and real precipitation was studied. According to different precipitation,change trends of precipitable water vapor in convective precipitation and steady precipitation were analyzed. Results showed that necessary condition of precipitation generation was high precipitable water vapor value in the air. Precipitable water vapor change presented wave-shape and phased characters. In convection precipitation,precipitable water vapor changed frequently and had larger change amplitude,while its change was slow in steady precipitation. The appearing time of the maximum values of rainfall intensity and precipitable water vapor was not necessarily consistent,but it was known that severe rainfall usually began at the high-value stage of precipitable water vapor,and high-value stage of precipitable water vapor often corresponded to higher precipitation probability. In addition,precipitable water vapor showed different characteristics in the above two different precipitation,and these results could provide a reference for precipitation forecast. | Hao Ling Zhao Liang Zhu Minhua Bai Xue Ma Chenchen | 2015 | Meteorological and Environmental Research2015，6，7： | 0 |
| 20 | Variation in Deformation Behaviors Along the Transverse Direction During the Warm Rolling of a 1480-mm-Wide AZ31B Plate显示文摘A decrease in the weight of aerospace vehicles,large ships,weapons,and high-speed trains will increase the demand for wide-width magnesium alloy plates and their composite parts to replace steel and plastic.An investigation was conducted to study the variation in deformation behaviors along the transverse direction during the warm rolling of a 1480-mm-wide AZ31B plate.A uniaxial thermal compression test with a 59%reduction was performed at different positions on a 13.7-mm-thick rolled plate along the width direction at a temperature of 220℃ and 270℃ and strain rate of 15 s^(−1).At the same time,the 13.7-mm-thick plate was rolled in a single pass to 5.6 mm on a mill with a 1725-mm-wide roll to confirm the thermal deformation behavior and the dynamic recrystallization(DRX).The results show that the main texture type does not change and the grain size does not have a clear deflection when the magnesium alloy plate reaches a certain value under rolling accumulative reduction.The grain size of a 13.7-mm-thick plate increases with a decrease in the distance to the center layer in the thickness direction.In the width direction,the edge(R6)first decreases and then increases toward the symmetric plane(R1).The critical stress required for dynamic recrystallization in the transition zone R3 of the rolled plate width is minimum,and the average grain size is minimum owing to the relatively complete recrystallization. | Chenchen Zhi Lifeng Ma Weitao Jia Pengtao Liu Qichi Le Zhiquan Huang Tingzhuang Han | 2021 | Chinese Journal of Mechanical Engineering2021，34，5： | 0 |
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