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| 1 | p53 upregulated by HIF-la promotes hypoxiainduced G2/M arrest and renal fibrosis in vitro and in vivo显示文摘Hypoxia plays an important role in the genesis and progression of renal fibrosis.The underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis. | Limin Liu Peng Zhang Ming Bai Lijie He Lei Zhang Ting Liu Zhen Yang Menglu Duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun | 2019 | Journal of Molecular Cell Biology2019，11，5： | 18 |
| 2 | Effect of methamidophos on soil fungi community in microcosms by plate count,DGGE and clone library analysis显示文摘Methamidophos 广泛地被使用在北中国的杀虫剂。在黑色的真菌的社区玷污的土壤上的 methamidophos 的潜在的影响被板计数, 28S rDNA-PCR-DGGE,和克隆图书馆分析估计。三个 methamidophos 层次(50,150,和 250 mg/kg ) 在土壤缩影被测试。从板计数的结果在一个 60-d 缩影实验期间证明 methamidophos ( 250 mg/kg )的高集中能显著地刺激采指纹显示出的模式的真菌的 populations.DGGE (使中毒的坡度胶化电气泳动)在 methamidophos.Shannon 差异索引的压力下面的 culturable 和全部的真菌社区的回答之间的重要差别从 DGGE 计算了侧面显示在有 methamidophos 处理的所有缩影的 culturable 真菌在 1 星期孵化以后增加了。然而,全部的真菌的差异索引在第一个星期内减少了,作为与 culturable 真菌的刺激相比。然而,在第 8 星期, methamidophos 对待的所有缩影类似于在由香农建议差异为 culturable 和总数索引真菌的社区结构的控制缩影。相反,在 1 个星期以后, culturable 和 unculturable 的真菌的结构被聚类分析两个都在 methamidophos 的压力下面扰乱到不同程度。克隆定序分析显示了刺激病原并且 unculturable 由 methamidophos 处理的真菌的人口,植物病爆发的 suggetsing 潜力风险。 | LI Xinyu ZHANG Huiwen WU Minna ZHANG Yan ZHANG Chenggang | 2008 | Journal of Environmental Sciences2008，20，5： | 11 |
| 3 | Ultrasound-Guided Transmuscular Quadratus Lumbar Block Reduces Opioid Consumption after Laparoscopic Partial Nephrectomy显示文摘Objectives Transmuscular quadratus lumborum block(TQLB)may provide postoperative analgesia in patients undergoing intraperitoneal surgeries.The purpose of this study was to examine the potential efficacy of TQLB among patients undergoing retroperitoneal procedures,such as the laparoscopic partial nephrectomy(LPN).Methods This prospective,randomized,controlled study was conducted from August 2017 to November 2018 at Peking Union Medical College Hospital(Beijing,China).Patients who were scheduled for a LPN,aged 18・70 years old with an ASA physical status score of I-II were randomly assigned to receive either TQLB with 0.6 ml/kg of 0.5%ropivacaine plus general anesthesia(TQLB group)or general anesthesia alone(control group).Patient-controlled intravenous analgesia with morphine was initiated immediately upon surgery completion.The primary outcome was the cumulative consumption of morphine within 8 h after surgery.The secondary outcome included postoperative consump廿ons of morphine at other time points,pain score at rest and during activity,postoperative nausa and vomitting(PONV),and recovery related parameters.Results Totally 30 patients per group were recruited in the study.The 8 h consumption of morphine was lower in theTQLB group than in the control group(median,0.023 mg/kg vs.0.068 mg/kg,U=207.5,P<0.001).No significant differences were observed in postoperative pain scores between the two groups.Patients in the TQLB group had fewer episodes of PONV(20%vs.47%,χ2=4.&P=0.028)in the first 24 h after surgery and higher scores for quality of recovery(mean,13&6 vs.131.9,t=-2.164,P=0.035)120 h after surgery than the controls.Conclusions TQLB resulted in an opioid-sparing effect during the early postoperative period following LPN,as well as a lower incidence of PONV and improved quality of recovery. | Xulei Cui Xu Li Minna Li Yuelun Zhang Yi Xie Weigang Yan Yushi Zhang Zhigang Ji Yuguang Huang | 2020 | Chinese Medical Sciences Journal2020，35，4： | 10 |
| 4 | Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils显示文摘Acetochlor is an increasingly used herbicide on corn in North China. Currently, the effect of acetochlor on soil ammonia-oxidizing bacteria (AOB) communities is not well documented. Here, we studied the diversity and community composition of AOB in soil amended with three concentrations of acetochlor (50, 150, 250 mg/kg) and the control (0 mg acetochlor/kg soil) in a microcosm experiment by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and the phylogenetic analysis of excised DGGE bands. DGGE profiles showed that acetochlor had a stimulating effect on AOB at the early stage after acetochlor amended, and the order of intensity and duration is medium-acetochlor amended samples (AM) > low-acetochlor amended samples (AL) > high-acetochlor amended samples (AH). At the end of 60 d microcosm, acetochlor had a negative effect on the diversity of AOB. Cluster analysis of DGGE profiles showed that acetochlor had a greater effect on the community structure of AOB on day 60 than on day 1. The phylogenetic analysis revealed that all the sequences of excised DGGE bands were closely related to members of the genus Nitrosospira and formed two separate subclusters designated as subcluster 1 and subcluster 2 affiliated respectively with clusters 3 and 4 in Nitrosospira as defined by Stephen. Some dominant AOB had a change from subcluster 2 to subcluster 1 with the incubation. The results showed that acetochlor had an effect on the AOB on a long-term basis and the chronic effect of acetochlor should be paid more attention in future. | Xinyu LI Chenggang ZHANG | 2008 | Journal of Environmental Sciences2008，20，9： | 7 |
| 5 | Soil fungistasis and its relations to soil microbial composition and diversity:A case study of a series of soils with different fungistasis显示文摘Fungistasis is one of the important approaches to control soil-borne plant pathogens.Some hypotheses about the mechanisms for soil fungistasis had been established,which mainly focused on the soil bacterial community composition,structure,diversity as well as function.In this study,the bacterial community composition and diversity of a series of soils treated by autoclaving,which coming from the same original soil sample and showing gradient fungistasis to the target soil-borne pathogen fungi Fusarium gr... | Wu Minna Zhang Huiwen Ll Xinyu Zhang Yan Su Zhencheng Zhang Chenggang | 2008 | Journal of Environmental Sciences2008，20，7： | 7 |
| 6 | The relative and combined effects of herbivore assemblage and soil nitrogen on plant diversity显示文摘Plant diversity can be affected by both herbivore grazing and soil resources. However, it is unclear if the joint effects of herbivores and soil resources might vary with components of plant diversity. Here, we evaluated the relative and combined effects of herbivore assemblage and soil nitrogen(N) quantity and heterogeneity on the α and β components of plant diversity in a grassland that was subjected to four years of grazing under differing herbivore assemblages(no grazing, cattle grazing, sheep grazing, and mixed grazing). We found that herbivore assemblage combined with soil N quantity explained 41% of the variation in plant α-diversity, while herbivore assemblage combined with soil N heterogeneity explained 15% of the variation in plant β-diversity. The independent effects of herbivore assemblage explained more than those of soil N for both α-and β-diversity(α-diversity: 12% vs. 4%;β-diversity: 18% vs. 16%). We concluded that the effects of herbivores are stronger than those of soil N,and that grazing-induced changes in soil resources are important drivers of plant diversity change, especially α-diversity.Therefore, we suggest that managing herbivore species by accounting for the effects that their grazing can have on soil resources may be significant for plant diversity maintenance. | Minna Zhang Bai Liu Guangyin Li Yingying Kuang Xiuquan Yue Shicheng Jiang Jushan Liu Ling Wang | 2022 | Science China(Life Sciences)2022，65，4： | 1 |
| 7 | hsa_circ_0056856 in the serum serves as a potential novel biomarker for disease activity in psoriasis显示文摘To the Editor:Psoriasis is a chronic immune-related inflammatory skin disease.The clinical severity of psoriasis varies greatly,and recurrence occurs frequently.[1]Many attempts have been made to identify biomarkers for psoriasis;however,there are currently no specific markers that can accurately monitor disease severity and treatment efficacy.Circular RNAs(circRNAs)are a novel class of endogenous noncoding RNAs that are highly stable and resistant to RNase R since they can form covalently closed loops without 50 caps and 30 tails.[2]It has been reported that circRNAs could exist in body fluids,such as serum and plasma,and could serve as molecular biomarkers in the diagnosis and prognosis of several diseases.[3]In this study,we detected the alteration of circRNAs and investigated the potential value of hsa_circ_0056856 as a biomarker for disease severity in serum samples from psoriasis patients and healthy controls(HCs). | Mingwang Zhang Minna Han Jian Li Kang Tao Xin Yang Haihao Zheng Rongya Yang Zhifang Zhai | 2022 | Chinese Medical Journal2022，，14： | 1 |
| 8 | Large-scale estimates of gross primary production on the Qinghai-Tibet plateau based on remote sensing data显示文摘Vegetation gross primary production(GPP)is an important variable for the carbon cycle on the Qinghai-Tibetan Plateau(QTP).Based on the measurements from 12 eddy covariance flux sites,we validated a light use efficiency model(i.e.EC-LUE)to evaluate the spatial-temporal patterns of GPP and the effect of environmental variables on QTP.In general,EC-LUE model performed well in predicting GPP at different time scale over QTP.Annual GPP over the entire QTP ranged from 575 to 703 Tg C,and showed a significantly increasing trend from 1982 to 2013.However,there were large spatial heterogeneities in long-term trends of GPP.Throughout the entire QTP,air temperature increase had a greater influence than solar radiation and precipitation(PREC)changes on productivity.Moreover,our results highlight the large uncertainties of previous GPP estimates due to insufficient parameterization and validations.When compared with GPP estimates of the EC-LUE model,most Coupled Model Intercomparison Project(CMIP5)GPP products overestimate the magnitude and increasing trends of regional GPP,which potentially impact the feedback of ecosystems to regional climate changes. | Minna Ma Wenping Yuan Jie Dong Fawei Zhang Wenwen Cai Hongqin Li | 2018 | International Journal of Digital Earth2018，11，11： | 0 |
| 9 | Prevalence and determinations of physical inactivity among public hospital employees in Shanghai, China: a cross-sectional study显示文摘这研究试图在上海在医院雇员之中探索物理迟钝的流行和决心,中国。19 ~ 68 年的 4612 个雇员的代表性的研究在 2011 从上海医院的不同的班通过成层的簇采样被进行。全部的物理活动根据全球物理活动问询表用新陈代谢的等价物被评估。在参加者之中, 38.5% , 32.3% ,和 64.6% 雇员在工作是不活跃的,变换,并且分别地花空闲时间。多达 41.8% 男人和 37.8% 女人(P = 0.012 ) 身体上是不活跃的。当年龄和教育水平被调整时,男医生和医药技术员在后勤比男工人显示出物理迟钝的一个更高的百分比(P = 0.001 ) 。在女性之中,雇员在二等车和三等车医院表演正在工作物理迟钝的一个更高的比例比在社区护理正在工作的那些集中。逻辑回归分析证明物理迟钝在工作与物理活动的紧张联系了的空闲时间的机会比率(ORs ) 为男人是 2.259, 2.897,和 4.266 (P P P < 0.001 ) 。医院雇员,特别地医生和医药技术员,在上海比另外的居民显示出物理迟钝的一个更高的比例。在工作的活动的时间和紧张并且变换与空闲时间活动被联系。 | Xinjian Li Minna Cheng Hao Zhang Ting Ke Yisheng Chen | 2015 | Frontiers of Medicine2015，9，1： | 0 |
| 10 | Effects of guanidinium cations on structural,optoelectronic and photovoltaic properties of perovskites显示文摘Guanidinium(GA)cations are intentionally introduced in MAPbI_(3) perovskite by considering its potential capability of stabilizing the material through plenty of hydrogen bonds and mitigating hysteresis because of the zero dipole moment.The configurations of GA cation in film and its effects on structural,optoelectronic and photovoltaic properties of perovskite have been comprehensively studied by systematically modulating the GA ratio.It has been demonstrated that moderate GA cations can effectively passivate the defect surrounding perovskite grains,yielding an enhanced efficiency as high as~19,2%in a p-i-n type planar solar cells with the GA ratio of 15%.Further increasing the GA ratio deteriorates device performance,as extra GA cations hinder grain growth and thus reduce the grain size,which facilitates the defect generation around the enhanced interface.Moreover,a new two-dimensional(2 D)layered perovskite phase that features alternating GA and MA cations in the interlayer space(ACI)appears ultimately,while the ACI phase typically suffers from slow charge transportation across the parallel PbI2 octahedral layers separated by large A-site cations. | Yi Ding Yan Wu Ying Tian Yuzeng Xu Minna Hou Bo Zhou Jingshan Luo Guofu Hou Ying Zhao Xiaodan Zhang | 2021 | Journal of Energy Chemistry2021，30，7： | 0 |
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