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| 1 | Classifying genotype F of hepatitis B virus into F1 and F2 subtypes显示文摘AIM: To explore the propriety of providing hepatitis B virus(HBV) genotypes F and H with two distinct genotypes.METHODS: Eleven HBV isolates of genotype F (HBV/F)were recovered from patients living in San Francisco,Japan, Panama, and Venezuela, and their full-length sequences were determined. Phylogenetic analysis was carried out among them along with HBV isolates previously reported.RESULTS: Seven of them clustered with reported HBV/F isolates in the phylogenetic tree constructed on the entire genomic sequence. The remaining four flocked on another branch along with three HBV isolates formerly reported as genotype H. These seven HBV isolates, including the four in this study and the three reported, had a sequence divergence of 7.3-9.5% from the other HBV/F isolates,and differed by > 13.7% from HBV isolates of the other six genotypes (A-E and G). Based on a marked genomic divergence, falling just short of >8% separating the seven genotypes, these seven HBV/F isolates were classified into F2 subtype and the former seven into F1 subtype provisionally. In a pairwise comparison of the S-gene sequences among the 7 HBV/F2 isolates and against 47HBV/F1 isolates as well as 136 representing the other six genotypes (A-E and G), two clusters separated by distinct genetic distances emerged.CONCLUSION: Based on these analyses, classifying HBV/F isolates into two subtypes (F1 and F2) would be more appropriate than providing them with two distinct genotypes (F and H). | Hideaki Kato Kei Fujiwara Robert G. Gish Hiroshi Sakugawa Hiroshi Yoshizawa Fuminaka Sugauchi Etsuro Orito Ryuzo Ueda Yasuhito Tanaka Takanobu Kato Yuzo Miyakawa Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，40： | 6 |
| 2 | Effect of roselle calyx extract on in vitro viability and biofilm formation ability of oral pathogenic bacteria显示文摘Objective: To investigate the effect of the roselle calyx extract(RCE)(Hibiscus sabdariffa L.) on the in vitro viability and biofilm formation ability of oral pathogenic bacteria. Methods:RCE was prepared by soaking roselle calyx powder with ethyl alcohol for 24 h at room temperature. After centrifugation, the extract was lyophilized. Then, the extract was dissolved in phosphate-buffered saline, the p H was adjusted, and the extract was aseptically filtered. We used Streptococcus mutans, Streptococcus sanguinis, Lactobacillus casei, Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in this study. The antibacterial activity of the RCE was determined by treating the cells of these bacteria with the extract for 10 or 20 min at room temperature. The minimum inhibitory concentration(MIC) and minimum bactericidal concentration was determined using the micro dilution method, and the effect of the RCE on the ability to form biofilm was determined using a polystyrene micro plate assay. In addition, we used the WST-1 assay to determine the cytotoxicity of the RCE on HGF, Ca9-22 and KB cells. Results: The RCE had antibacterial activity against oral bacteria used in this study. In particular, most significant antibacterial activity was observed against Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The MIC and minimum bactericidal concentration were 7.2 mg/m L–28.8 mg/m L and 14.4 to >57.6 mg/m L. The RCE had an inhibitory effect on biofilm formation at the MIC and sub-MIC levels. In addition, the RCE had low cytotoxic effects on HGF, Ca9-22 and KB cells. Conclusions: Thus, our results indicate that the RCE may be used for preventing oral diseases. | Herastuti Sulistyani Mari Fujita Hiroshi Miyakawa Futoshi Nakazawa | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，2： | 3 |
| 3 | Pancreaticobiliary maljunction and carcinogenesis to biliary and pancreatic malignancy显示文摘 | Takahiko Funabiki Toshiki Matsubara Shuichi Miyakawa Shin Ishihara | 2009 | Langenbeck’s Archives of Surgery2009，，1： | 3 |
| 4 | Operative Results and Postoperative Progression of Ossification Among Patients With Ossification of Cervical Posterior Longitudinal Ligament显示文摘 | KIYOSHI HIRABAYASHI JUN MIYAKAWA KAZUHIKO SATOMI TETSUO MARUYAMA KOICHI WAKANO | 1981 | Spine1981，，4： | 2 |
| 5 | A Novel DNA Virus (TTV) Associated with Elevated Transaminase Levels in Posttransfusion Hepatitis of Unknown Etiology显示文摘 | Tsutomu Nishizawa Hiroaki Okamoto Keiko Konishi Hiroshi Yoshizawa Yuzo Miyakawa Makoto Mayumi | 1997 | Biochemical and Biophysical Research Communications1997，，1： | 2 |
| 6 | SPATA17的单核苷酸多态性可能是日本减速分裂阻滞患者的遗传危险因素显示文摘遗传机制已经成为一些男性患者不育的一个病因。近年来,通过人类睾丸组织的基因芯片分析鉴定了10个与人类精子发生相关的新基因。其中一个是精子发生相关17基因(SPATA17)。为了研究是否SPATA17基因缺陷与减数分裂导致的无精症有关,我们进行了突变分析,将18个日本减数分裂导致的无精症患者的SPATA17编码区域进行了测序,以及统计分析,其中包括18个减数分裂阻滞患者,20个唯支持细胞综合征(SCOS)患者和96个健康对照男性。没有SPATA17发生突变。但是,减数分裂阻滞患者组检测到了3个单核苷酸多态性(cSNPs:SNPI—SNP3)。减数分裂阻滞患者与其他患者的SNP1和SNP2基因型与等位基因频率无明显不同。但是,SNP3的等位基因频率在减数分裂阻滞患者组中明显升高(P〈0.05)。这项研究表明,sPATA17在人类精子发生,尤其是减数分裂中可能起关键性作用。 | Toshinobu Miyamoto Akira Tsujimura Yasushi Miyagawa Eitetsu Koh Naoko Sakugawa Hiroe Miyakawa Hisashi Sato Mikio Namiki Akihiko Okuyama Kazuo Sengoku | 2009 | Asian Journal of Andrology2009，11，5： | 2 |
| 7 | Poor Response to Pegylated Interferon and Ribavirin in Older Women Infected with Hepatitis C Virus of Genotype 1b in High Viral Loads显示文摘 | Hitomi Sezaki Fumitaka Suzuki Yusuke Kawamura Hiromi Yatsuji Tetsuya Hosaka Norio Akuta Masahiro Kobayashi Yoshiyuki Suzuki Satoshi Saitoh Yasuji Arase Kenji Ikeda Yuzo Miyakawa Hiromitsu Kumada | 2009 | Digestive Diseases and Sciences2009，，6： | 2 |
| 8 | Association of two polymorphisms of the IL28B gene with viral factors and treatment response in 1,518 patients infected with hepatitis C virus显示文摘 | Mariko Kobayashi Fumitaka Suzuki Norio Akuta Hitomi Sezaki Yoshiyuki Suzuki Tetsuya Hosaka Yusuke Kawamura Masahiro Kobayashi Satoshi Saitoh Yasuji Arase Kenji Ikeda Kazuaki Chayama Yuzo Miyakawa Hiromitsu Kumada | 2012 | Journal of Gastroenterology2012，，5： | 2 |
| 9 | Purification, characterization, and molecular gene cloning of an antifungal protein from Ginkgo biloba seeds显示文摘 | Yoriko Sawano Takuya Miyakawa Hiroshi Yamazaki Masaru Tanokura Ken-ichi Hatano | 2007 | Biological Chemistry2007，，3： | 2 |
| 10 | The revised Japanese clinical diagnostic criteria for chronic pancreatitis显示文摘 | Tooru Shimosegawa Keisho Kataoka Terumi Kamisawa Hiroyuki Miyakawa Hirotaka Ohara Tetsuhide Ito Satoru Naruse Naohiro Sata Koichi Suda Morihisa Hirota Yoshifumi Takeyama Keiko Shiratori Takashi Hatori Makoto Otsuki Yutaka Atomi Kentaro Sugano Masao Tanaka | 2010 | Journal of Gastroenterology2010，，6： | 2 |
| 11 | Guidelines for the management of biliary tract and ampullary carcinomas: surgical treatment显示文摘 | Satoshi Kondo Tadahiro Takada Masaru Miyazaki Shuichi Miyakawa Kazuhiro Tsukada Masato Nagino Junji Furuse Hiroya Saito Toshio Tsuyuguchi Masakazu Yamamoto Masato Kayahara Fumio Kimura Hideyuki Yoshitomi Satoshi Nozawa Masahiro Yoshida Keita Wada Satoshi | 2008 | Journal of Hepato - Biliary - Pancreatic Surgery2008，，1： | 2 |
| 12 | Pancreaticobiliary maljunction and carcinogenesis to biliary and pancreatic malignancy显示文摘 | Takahiko Funabiki Toshiki Matsubara Shuichi Miyakawa Shin Ishihara | 2009 | Langenbeck’s Archives of Surgery2009，，1： | 2 |
| 13 | Detection of antimito chondrial autoant ibodies in immunofluorescent AMA-negative patients with primary biliary cirrhos is using recombinant autoantigens显示文摘 | Miyakawa H Tanaka A Kikuchi K | | 0，，： | 2 |
| 14 | Prevalence of hepatitis C virus variants resistant to NS3 protease inhibitors or the NS5A inhibitor (BMS-790052) in hepatitis patients with genotype 1b显示文摘 | Fumitaka Suzuki Hitomi Sezaki Norio Akuta Yoshiyuki Suzuki Yuya Seko Yusuke Kawamura Tetsuya Hosaka Masahiro Kobayashi Satoshi Saito Yasuji Arase Kenji Ikeda Mariko Kobayashi Rie Mineta Sachiyo Watahiki Yuzo Miyakawa Hiromitsu Kumada | 2012 | Journal of Clinical Virology2012，，4： | 2 |
| 15 | Neurogranin null mutant mice display performance deficits on spatial learning tasks with anxiety related components显示文摘 | Miyakawa T Yared E Pak JH | 2001 | Hippoeampus2001，11，6： | 1 |
| 16 | Detection of antimitochondrial autoantibodies in immunofluurescent AMAM2 negative patients with primary biliary cirrhosis using recombinant autoantigcns显示文摘 | Miyakawa H Tanaka A Kikuchi K | 2001 | Hcpatology2001，34，： | 1 |
| 17 | Krasmutation in tamoxffen-related endometrial polyps 显示文摘 | Hachisuga T Miyakawa T Tsujioka H | 2003 | Cancer2003，98，9： | 1 |
| 18 | Gene up-regulation in resPonse to predator kairomones in the water flea, Daphnia pulex 显示文摘 | Miyakawa H Maki I Naoki S | 2010 | BMC Developmental Biology2010，10，1： | 1 |
| 19 | Diagnosis of biliary tract and ampullary carcinomas显示文摘 | Kazuhiro Tsukada Tadahiro Takada Masaru Miyazaki Shuichi Miyakawa Masato Nagino Satoshi Kondo Junji Furuse Hiroya Saito Toshio Tsuyuguchi Fumio Kimura Hideyuki Yoshitomi Satoshi Nozawa Masahiro Yoshida Keita Wada Hodaka Amano Fumihiko Miura | 2008 | Journal of Hepato - Biliary - Pancreatic Surgery2008，，1： | 1 |
| 20 | The enterotoxic Clostridia显示文摘 | MCCLANE B A UZAL F A MIYAKAWA M E F | 2006 | Prokaryotes2006，4，： | 1 |
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