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| 1 | Chemical kinetics study of hydrocarbon regeneration from organic matter in carbonate source rocks and its significance显示文摘In the comparison research of hydrocarbon regeneration, a low maturity carbonate source rock is heated to different temperatures in a gold tube to obtain a series of samples with different maturities. Then, the heated samples, before and after extraction, are subjected to Rock-Eval pyrolysis through a thermal simulation of hydrocarbon regeneration in order to inspect pyrolysis characteristics and probe into the characteristics of the chemical kinetics of each sample. The results indicate that, whether hy- drocarbon regeneration peak is delayed or advanced, the potential of hydrocarbon regeneration is closely related to the expulsion amount and breakdown maturity of primary hydrocarbon generation. After extraction, the average activation energy of artificially maturated samples increases with the in- creasing maturity, but the chemical kinetic properties of un-extracted samples decrease. The calibrated chemical kinetic models that describe extracted and un-extracted samples are applied to the Bohai Bay and the Songliao Basin, and the results indicate that the combination of the two models can explain some contradictory conclusions previously reported. These results also facilitate the quantitative evaluation of the amount of hydrocarbon regeneration by the chemical kinetic method. | LU ShuangFang ZHONG NingNing XUE HaiTao PAN ChangChun LI JiJun LI HongTao | 2007 | Science China Earth Sciences2007，50，4： | 8 |
| 2 | MIKC^(C)-type MADS-box genes in Rosa chinensis: the remarkable expansion of ABCDE model genes and their roles in floral organogenesis显示文摘MIKC^(C)-type MADS-box(MIKC^(C))genes encode transcription factors that have crucial roles in controlling floral organogenesis and flowering time in plants.Although this gene family has been well characterized in many plant species,its evolutionary and comprehensive functional analysis in rose is lacking.In this study,58 non-redundant MIKC^(C)uni-transcripts were extensively identified from rose transcriptomes.Phylogenetic analysis placed these genes into 12 clades with their Arabidopsis and strawberry counterparts,and revealed that ABCDE model(including AP1/FUL,AP3/PI,AG,and SEP clades),and SOC1 and AGL6 clade genes have remarkably expanded in Rosa chinensis,whereas genes from the FLC and AGL17 clades were undetectable.Sequence alignments suggest that the AP3/PI clade may contribute to more specific functions in rose due to a high variation of amino acid residues within its MADS-box domains.A comparative analysis of gene expression in specific floral organ differentiation stages and floral organs between R.chinensis cv.Old Blush and the closely related mutant genotype R.chinensis cv.Viridiflora(floral organs mutated into leaf-like structures)further revealed the roles of ABCDE model genes during floral organogenesis in rose.Analysis of co-expression networks provided an overview of the regulatory mechanisms of rose MIKC^(C)genes and shed light on both the prominent roles of AP3/PI clade genes in floral organogenesis and the roles of RcAGL19,RcAGL24,and RcSOC1 in regulating floral transition in rose.Our analyses provide an overall insight of MIKC^(C)genes in rose and their potential roles in floral organogenesis. | Jinyi Liu Xiaodong Fu Yuwei Dong Jun Lu Min Ren Ningning Zhou Changquan Wang | 2018 | Horticulture Research2018，5，1： | 7 |
| 3 | Q-switching of waveguide lasers based on graphene/WS_2 van der Waals heterostructure显示文摘We report on the operation of passively Q-switched waveguide lasers at 1 μm wavelength based on a graphene∕WS_2 heterostructure as a saturable absorber(SA). The gain medium is a crystalline Nd:YVO_4 cladding waveguide produced by femtosecond laser writing. The nanosecond waveguide laser operation at 1064 nm has been realized with the maximum average output power of 275 m W and slope efficiency of 37%. In comparison with the systems based on single WS_2 or graphene SA, the lasing Q-switched by a graphene∕WS_2 heterostructure SA possesses advantages of a higher pulse energy and enhanced slope efficiency, indicating the promisingapplications of van der Waals heterostructures for ultrafast photonic devices. | ZIQI LI CHEN CHENG NINGNING DONG CAROLINA ROMERO QINGMING LU JUN WANG JAVIER RODRíGUEZ VáZQUEZ DE ALDANA YANG TAN FENG CHEN | 2017 | Photonics Research2017，5，5： | 7 |
| 4 | Defect inspection of flip chip package using SAM technology and fuzzy C-means algorithm显示文摘Solder bumps are widely used in surface mount components, which provide electrical and mechanical connection between the chip/package and the substrate. As the solder bump getting smaller in dimension and pitch, it becomes more difficult to inspect the solder defects hidden in the IC package. In this paper, an intelligent inspection method using the scanning acoustic microscopy(SAM) and the fuzzy C-means(FCM) algorithm was investigated. A flip chip package of FA10 was chosen as the test sample. The SAM tests of FA10 were carried out in C-scan mode. The sub-image of every solder bump was segmented from the SAM image. The statistical features were then calculated and adopted for clustering of solder bumps using the FCM algorithm. The recognition results of FCM reached a high accuracy of 94.3%. The intelligent system is effective for defect inspection in high density packages. | LU XiangNing LIU Fan HE ZhenZhi LI LiYi HU NingNing SU Lei | 2018 | Science China(Technological Sciences)2018，61，9： | 4 |
| 5 | Hybrid speciation via inheritance of alternate alleles of parental isolating genes显示文摘It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of speciation.HHS will likely increase in frequency as ecological and geographical barriers between species are continuing to be disrupted by human activities.HHS requires the establishment of reproductive isolation between a hybrid and its parents,but the underlying genes and genetic mechanisms remain largely unknown.In this study,we reveal by integrated approaches that reproductive isolation originates in one homoploid hybrid plant species through the inheritance of altemate alleles at genes that determine parental premating isolation.The parent species of this hybrid species are reproductively isolated by differences in flowering time and survivorship on soils containing high concentrations of iron.We found that the hybrid species inherits alleles of parental isolating major genes related to flowering time from one parent and alleles of major genes related to iron tolerance from the other parent.In this way,it became reproductively isolated from one parent by the difference in flowering time and from the other by habitat adaptation(iron tolerance).These findings and further modeling results suggest that HHS may occur relatively easily via the inheritance of alternate parental premating isolating genes and barriers. | Zefu Wang Yuanzhong Jiang Hao Bi Zhiqiang Lu Yazhen Ma Xiaoyue Yang Ningning Chen Bin Tian Bingbing Liu Xingxing Mao Tao Ma Stephen P.DiFazio Quanjun Hu Richard J.Abbott Jianquan Liu | 2021 | Molecular Plant2021，14，2： | 4 |
| 6 | Physiological levels of ATP negatively regulate proteasome function显示文摘由 ubiquitin-proteasome 系统的细胞内部的蛋白质降级是 ATP 依赖者,和最佳的 ATP 集中激活 proteasome 在 vitro 的功能是 ~ 100 渭 M。细胞内部的 ATP 层次在在这个范围以内的水平通常在低 millimolar 范围,而是 ATP 被显示在 vitro 禁止 proteasome peptidase 活动。这里,我们报导支持在生理的层次的细胞内部的 ATP 双向地调整的一个假设的新证据 26S proteasome 在房间的解朊的功能。首先,我们证实 ATP 在 vitro 在 26S proteasome 上施加了双向规定,与最佳的 ATP 集中(在 50 和 100 渭 M 之间) 刺激 proteasome 像糜蛋白酶的活动。第二,我们发现操作细胞内部的 ATP 层次也在有教养的房间在 proteasome 特定的蛋白质底层的层次导致了双向变化。最后,测量增加提高的细胞内部的 ATP,当减少的细胞内部的 ATP 稀释了导致房间死亡的 proteasome 抑制的能力时。这些数据强烈建议在生理的集中范围以内的内长的 ATP 能在 proteasome 活动施加否定影响,允许房间到很快,在应力下面的 ATP 减小上的 upregulate proteasome 活动调节。 | Hongbiao Huang Xiaoyan Zhang Shujue Li Ningning Liu Wen Elan Emily McDowell Ping Zhou Canguo Zhao Haiping Guo Change Zhang Changshan Yang Guangmei Wen Xiaoxian Dong Li Lu Ningfang Ma Weihua Dong Q Ping Dou Xuejun Wang Jinbao Liu | 2010 | Cell Research2010，20，12： | 4 |
| 7 | Regulation of Leaf Longevity by DML3-Mediated DNA Demethylation显示文摘Leaf senescence is driven by the expression of senescence-associated genes(SAGs).Development-specific genes often undergo DNA demethylation in their promoter and other regions,which regulates gene expression.Whether and how DNA demethylation regulates the expression of SAGs and thus leaf senescence remain elusive.Whole-genome bisulfite sequencing(WGBS)analyses of wild-type(WT)and demeter-like 3(dml3)Arabidopsis leaves at three developmental stages revealed hypermethylation during leaf senescence in dml3 compared with WT,and 20556 differentially methylated regions(DMRs)were identified by comparing the methylomes of dml3 and WT in the CG,CHG,and CHH contexts.Furthermore,we identified that 335 DMR-associated genes(DMGs),such as NAC016 and SEN1,are upregulated during leaf senescence,and found an inverse correlation between the DNA methylation levels(especially in the promoter regions)and the transcript abundances of the related SAGs in WT.In contrast,in dml3 the promoters of SAGs were hypermethylated and their transcript levels were remarkably reduced,and leaf senescence was significantly delayed.Collectively,our study unraveled a novel epigenetic regulatory mechanism underlying leaf senescence in which DML3 is expressed at the onset of and during senescence to demethylate promoter,gene body or 3'UTR regions to activate a set of SAGs. | Lu Yuan Dan Wang Liwen Cao Ningning Yu Ke Liu Yongfeng Guo Susheng Gan Liping Chen | 2020 | Molecular Plant2020，13，8： | 3 |
| 8 | Integrated single-cell RNA sequencing analysis reveals distinct cellular and transcriptional modules associated with survival in lung cancer显示文摘Lung adenocarcinoma(LUAD)and squamous carcinoma(LUSC)are two major subtypes of non-small cell lung cancer with distinct pathologic features and treatment paradigms.The heterogeneity can be attributed to genetic,transcriptional,and epigenetic parameters.Here,we established a multi-omics atlas,integrating 52 single-cell RNA sequencing and 2342 public bulk RNA sequencing.We investigated their differences in genetic amplification,cellular compositions,and expression modules.We revealed that LUAD and LUSC contained amplifications occurring selectively in subclusters of AT2 and basal cells,and had distinct cellular composition modules associated with poor survival of lung cancer.Malignant and stage-specific gene analyses further uncovered critical transcription factors and genes in tumor progression.Moreover,we identified subclusters with proliferating and differentiating properties in AT2 and basal cells.Overexpression assays of ten genes,including sub-cluster markers AQP5 and KPNA2,further indicated their functional roles,providing potential targets for early diagnosis and treatment in lung cancer. | Li Zhang Yiming Zhang Chengdi Wang Ying Yang Yinyun Ni Zhoufeng Wang Tingting Song Menglin Yao Zhiqiang Liu Ningning Chao Yongfeng Yang Jun Shao Zhidan Li Ran Zhou Li Chen Dan Zhang Yuancun Zhao Wei Liu Yupeng Li Ping He Jing-wen Lin Yuan Wang Kang Zhang Lu Chen Weimin Li | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 2 |
| 9 | Synergistic Effects of External Electric Field and Solvent Vapor Annealing with Different Polarities to Enhance β-Phase and Carrier Mobility of the Poly(9,9-dioctylfluorene)Films显示文摘In this work,the synergistice efects of external electric field(EEF)and solvent vapor annealing to enhanceβ-phase and carrier mobility of poly(9,9-dioctylfuorene)(PFO)films were investigated.It is found that EEF can promote the PFOβ-phase conformation transition and orientate the PFO chains along the EEF direction with the as-sistance of polar solvent vapor annealing.PFO chain orderness is closely related to the solvent polarity.In particular,the B-phase content in the annealed film of strong polar chloroform vapor increases from 18.7%to 34.9%after EEF treatment.Meanwhile a characteristic needle-like crystal is formed in the flm,as a result,the hole mobility is en-hanced by an order of magnitude.The mechanism can be attributed to the fast polarization of solvent dipole under the action of EEF,thus forming a driving force that greatly facilitates the orientation of PFO dipole unit.Research also reveals that EEF driving of the PFO chains does not occur with an insoluble solvent vapor since the solvent molecules cannot swell the film,thus there is insufficient free volume for PFO chains to adjust their conformation.This research enriches the understanding of the relationship between solvent vapor annealing and EEF in orientation polymers,and this method is simple and controlled,and capable of integrating into large-area thin film process,which provides new insights to manufacture low-cost and highly ordered polymer films,and is of great significance to enhance carrier mobility and efficiency of photoelectric devices based on polymer condensed matter physics. | MATengning SONG Ningning QIU Jing ZHANG Hao LU Dan | 2020 | Chemical Research in Chinese Universities2020，36，6： | 2 |
| 10 | Nonvolatile waveguide transmission tuning with electrically-driven ultra-small GST phase-change material显示文摘Low-power reconfigurable optical circuits are highly demanded to satisfy a variety of different applications. Conventional electro-optic and thermo-optic refractive index tuning methods in silicon photonics are not suitable for reconfiguration of optical circuits due to their high static power consumption and volatility. We propose and demonstrate a nonvolatile tuning method by utilizing the reversible phase change property of GST integrated on top of the silicon waveguide. The phase change is enabled by applying electrical pulses to the lm-sized GST active region in a sandwich structure. The experimental results show that the optical transmission of the silicon waveguide can be tuned by controlling the phase state of GST. | Hanyu Zhang Linjie Zhou Jian Xu Ningning Wang Hao Hu Liangjun Lu B. M. A. Rahman Jianping Chen | 2019 | Science Bulletin2019，64，11： | 2 |
| 11 | Comparisons of stable isotopic fractionation in winter and summer at Baishui Glacier No.1,Mt.Yulong,China显示文摘Based on the data of δ18O in surface snow, snow pits, meltwater and the glacier-fed river water at Baishui Glacier No.1, Mt. Yulong, the isotopic fractionation behaviors in the typical monsoonal temperate glacier system in winter and summer were compared. The results indicate that the isotopic fractionation degree in summer is greater than that in winter, suggesting that the snow/ice melting is more intense in summer. Moreover, whenever it is in winter or summer, from surface snow to meltwater, and to glacier-fed river water, the gradient of δ18O with altitude gradually increases. This shows that the degree of isotopic fractionation gradually strengthens when surface snow is being converted into meltwater and finally into glacial river water, which suggests that the influence of post-depositional processes on δ18O gradient in the monsoonal temperate glacier region differs spatially. | PANG Hongxi HE Yuanqing LU Aigang ZHAO Jingdong NING Baoying YUAN Lingling SONG Bo ZHANG Ningning | 2006 | Journal of Geographical Sciences2006，16，3： | 2 |
| 12 | Down-staging depth score could be a survival predictor for locally advanced gastric cancer patients after preoperative chemoradiotherapy显示文摘Objective:The predictive effect of preoperative chemoradiotherapy(CRT)is low and difficult in guiding individualized treatment.We examined a surrogate endpoint for long-term outcomes in locally advanced gastric cancer patients after preoperative CRT.Methods:From April 2012 to April 2019,95 patients with locally advanced gastric cancer who received preoperative concurrent CRT and who were enrolled in three prospective studies were included.All patients were stage T_(3/4) N_(+).Local control,distant metastasis-free survival(DMFS),disease-free survival(DFS)and overall survival(OS)were evaluated.Clinicopathological factors related to long-term prognosis were analyzed using univariate and multivariate analyses.The down-staging depth score(DDS),which is a novel method of evaluating CRT response,was used to predict long-term outcomes.Results:The median follow-up period for survivors was 30 months.The area under the curve(AUC)of the receiver operating characteristic(ROC)curve predicted by the DDS was 0.728,which was better than the pathological complete response(pCR),histological response and ypN0.Decision curve analysis further affirmed that DDS had the largest net benefit.The DDS cut-off value was 4.pCR and ypN0 were associated with OS(P=0.026 and 0.049).Surgery and DDS are correlated with DMFS,DFS and OS(surgery:P=0.001,<0.001 and<0.001,respectively;and DDS:P=0.009,0.013 and 0.032,respectively).Multivariate analysis showed that DDS was an independent prognostic factor of DFS(P=0.021).Conclusions:DDS is a simple,short-term indicator that was a better surrogate endpoint than pCR,histological response and ypN0 for DFS. | Ning Li Xin Wang Yuan Tang Dongbin Zhao Yihebali Chi Lin Yang Liming Jiang Jun Jiang Jinming Shi Wenyang Liu Hua Ren Hui Fang Yu Tang Bo Chen Ningning Lu Hao Jing Shunan Qi Shulian Wang Yueping Liu Yongwen Song Yexiong Li Jing Jin | 2021 | Chinese Journal of Cancer Research2021，33，4： | 1 |
| 13 | Flexible multilayer lead-free film capacitor with high energy storage performances via heterostructure engineering显示文摘The immense potential of flexible energy storage materials applied in wearable electronic devices hasstimulated a lot of science researches on manufacturing technology and performance optimization.Herein, an all-inorganic flexible ferroelectric film with multilayer heterostructure is prepared based onMn doped Bi0.5Na0.5TiO_(3)eBiNi0.5Zr0.5O_(3) (Mn:BNT-BNZ) and Bi0.5Na0.5TiO_(3)eBiZn0.5Zr0.5O_(3) (BNT-BZZ)relaxor ferroelectrics. A win-win situation of breakdown strength and polarization is achieved in theMn:BNT-BNZ/BNT-BZZ multilayer film with the stacking period N - 3, of which energy density and ef-ficiency reach 80.4 J/cm3 and 62.0% respectively. It is proposed that the excellent energy storage per-formances are attributed to the synergistic effect of the electric field amplification effect, interfaceblocking effect and the polarization coupling effect based on the multilayer heterostructure. Moreover,the flexible ferroelectric film exhibits outstanding temperature (25e205 - C), frequency (0.5e5 kHz)stability and antifatigue property (1 108 cycles), and can well maintain stable performance at differenttensile/compressive bending radii (10e5 mm) and even after 104 bending cycles with a fixed bendingradius of 3 mm. This work opens up a promising route to the development of flexible energy storagematerials. | Ningning Sun Jinhua Du Ye Zhao Chunxiao Lu Pei Han Yong Li Qiwei Zhang Xihong Hao | 2022 | Journal of Materiomics2022，8，4： | 1 |
| 14 | Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition显示文摘 | Li Lu Aiping Qin Hongbiao Huang Ping Zhou Chuanyin Zhang Ningning Liu Shujue Li Guanmei Wen Change Zhang Weihua Dong Xuejun Wang Q. Ping Dou Jinbao Liu | 2011 | European Journal of Pharmacology2011，，2： | 1 |
| 15 | A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism显示文摘Calciphylaxis is a rare disease characterized histologically by microvessel calcification and microthrombosis,with high mortality and no proven therapy.Here,we reported a severe uremic calciphylaxis patient with progressive skin ischemia,large areas of painful malodorous ulcers,and mummified legs.Because of the worsening symptoms and signs refractory to conventional therapies,treatment with human amnion-derived mesenchymal stem cells(hAMSCs)was approved.Preclinical release inspections of hAMSCs,efficacy,and safety assessment,including cytokine secretory ability,immunocompetence,tumorigenicity,and genetics analysis in vitro,were introduced.We further performed acute and long-term hAMSC toxicity evaluations in C57BL/6 mice and rats,abnormal immune response tests in C57BL/6 mice,and tumorigenicity tests in neonatal Balbc-nu nude mice.After the preclinical research,the patient was treated with hAMSCs by intravenous and local intramuscular injection and external supernatant application to the ulcers.When followed up to 15 months,the blood-based markers of bone and mineral metabolism improved,with skin soft tissue regeneration and a more favorable profile of peripheral blood mononuclear cells.Skin biopsy after 1-month treatment showed vascular regeneration with mature noncalcified vessels within the dermis,and 20 months later,the re-epithelialization restored the integrity of the damaged site.No infusion or local treatment-related adverse events occurred.Thus,this novel long-term intravenous combined with local treatment with hAMSCs warrants further investigation as a potential regenerative treatment for uremic calciphylaxis due to effects of inhibiting vascular calcification,stimulating angiogenesis and myogenesis,anti-inflammatory and immune modulation,multidifferentiation,re-epithelialization,and restoration of integrity. | Lianju Qin Jing Zhang Yujie Xiao Kang Liu Yugui Cui Fangyan Xu Wenkai Ren Yanggang Yuan Chunyan Jiang Song Ning Xiaoxue Ye Ming Zeng Hanyang Qian Anning Bian Fan Li Guang Yang Shaowen Tang Zhihong Zhang Juncheng Dai Jing Guo Qiang Wang Bin Sun Yifei Ge Chun Ouyang Xueqiang Xu Jing Wang Yaoyu Huang Hongqing Cui Jing Zhou Meilian Wang Zhonglan Su Yan Lu Di Wu Jingping Shi Wei Liu Li Dong Yinbing Pan Baiqiao Zhao Ying Cui Xueyan Gao Zhanhui Gao Xiang Ma Aiqin Chen Jie Wang Meng Cao Qian Cui Li Chen Feng Chen Youjia Yu Qiang Ji Zhiwei Zhang Mufeng Gu Xiaojun Zhuang Xiaolin Lv Hui Wang Yanyan Pan Ling Wang Xianrong Xu Jing Zhao Xiuqin Wang Cuiping Liu Ningxia Liang Changying Xing Jiayin Liu Ningning Wang | 2022 | Journal of Molecular Cell Biology2022，14，2： | 1 |
| 16 | Structural insights into the membrane microdomain organization by SPFH family proteins显示文摘The lateral segregation of membrane constituents into functional microdomains,conceptually known as lipid raft,is a universal organization principle for cellular membranes in both prokaryotes and eukaryotes.The widespread Stomatin,Prohibitin,Flotillin,and HflK/C(SPFH)family proteins are enriched in functional membrane microdomains at various subcellular locations,and therefore were hypothesized to play a scaffolding role in microdomain formation.In addition,many SPFH proteins are also implicated in highly specific processes occurring on the membrane.However,none of these functions is understood at the molecular level.Here we report the structure of a supramolecular complex that is isolated from bacterial membrane microdomains and contains two SPFH proteins(HflK and HflC)and a membrane-anchored AAA+protease FtsH.HflK and HflC form a circular 24-mer assembly,featuring a laterally segregated membrane microdomain(20 nm in diameter)bordered by transmembrane domains of HflK/C and a completely sealed periplasmic vault.Four FtsH hexamers are embedded inside this microdomain through interactions with the inner surface of the vault.These observations provide a mechanistic explanation for the role of HflK/C and their mitochondrial homologs prohibitins in regulating membrane-bound AAA+proteases,and suggest a general model for the organization and functionalization of membrane microdomains by SPFH proteins. | Chengying Ma Chengkun Wang Dingyi Luo Lu Yan Wenxian Yang Ningning Li Ning Gao | 2022 | Cell Research2022，32，2： | 0 |
| 17 | Electricity Pricing with Continuous-time Commodity Model and Non-monotonic Load Curve显示文摘Under the 'carbon peak and carbon neutrality'background,clean energy enters the market on a large scale,so it is very important to study the pricing theory of electricity.The bright spot of this paper is that the load curve is more practical.In the original version of electricity pricing theory based on continuous time commodity model,the load curve is supposed to be a monotonic function with time for simplicity of derivation,which is not realistic.To overcome this defect,load curves are supposed to be in shapes of a unimodal model and multimodal cases in this paper,which are more in conformity with the actual load curve in real-world power markets.Two study cases are used to demonstrate the rationality of the proposed models.These results together with the authors'previous work provide a complete pricing scheme for continuous-time electricity commodity model. | Lijia Han Yue Li Haoyong Chen Ningning Lu | 2024 | CSEE Journal of Power and Energy Systems2024，10，1： | 0 |
| 18 | Molecular Cloning, Characterization and Sequence Analysis of KCNQ4 in Large Odorous Frog, Odorrana graminea显示文摘Acoustic communication is essential for anuran survival and reproduction, and masking background noise can affect the effective acoustic communication. The larger odorous frog(Odorrana graminea) inhabits noise montane streams, and it has shown an ultrasound communication adaptation. However, the molecular mechanism underlying their ultrasonic hearing adaptation remains unknown. To characterize and investigate the molecular characteristics and evolution of the high-frequency hearing-sensitive gene(KCNQ4) in O. graminea, termed as OgKCNQ4, the rapid amplification of cDNA ends(RACE) was performed to amplify the cDNA of OgKCNQ4. Different bioinformatics analyses were used to investigate the molecular characteristics. Multiple nucleotide and amino acid sequence alignment were conducted, and phylogenies were reconstructed under the maximum likelihood and Bayesian approaches. The full-length cDNA of OgKCNQ4 was 2065 bp, and the open reading frame(ORF) was 2046 bp encoding for a putative protein with 681 amino acids. The relative molecular weight of OgKCNQ4 was 76.453 kD and the putative PI was 9.69. Secondary structure prediction analyses suggested 42.29% alpha helixes and 43.76% random coils in OgKCNQ4. Gene homology and Phylogenetic analyses revealed the closest relationship between OgKCNQ4 and KCNQ4 of Nanorana parkeri with 96.9% similarity and 95.0% identity. We first determined the full-length cDNA of OgKCNQ4 and the results here could provide foundations for further study on the evolution of KCNQ4 and its relationship to ultrasonic communication in amphibians. | Ningning LU Ziwen GU Zhuo CHEN Xiaohong CHEN | 2019 | Asian Herpetological Research2019，10，4： | 0 |
| 19 | FDI in BRICs显示文摘 | Lu Ningning | 2009 | 南北桥2009，，10： | 0 |
| 20 | The most tenuous group query显示文摘Rtecently a lot of works have been investigating to find the tenuous groups,i.e.,groups with few social interactions and weak relationships among members,for reviewer selection and psycho-educational group formation.However,the metrics(e.g.,k-triangle,k-line,and k-tenuity)used to measure the tenuity,require a suitable k value to be specified which is difficult for users without background knowledge.Thus,in this paper we formulate the most tenuous group(MTG)query in terms of the group distance and average group distance of a group measuring the tenuity to eliminate the influence of parameter k on the tenuity of the group.To address the MTG problem,we first propose an exact algorithm,namely MTGVDIS,which takes priority to selecting those vertices whose vertex distance is large,to generate the result group,and also utilizes effective filtering and pruning strategies.Since MTGVDIS is not fast enough,we design an efficient exact algorithm,called MTG-VDGE,which exploits the degree metric to sort the vertexes and proposes a new combination order,namely degree and reverse based branch and bound(DRBB).MTG-VDGE gives priority to those vertices with small degree.For a large p,we further develop an approximation algorithm,namely MTG-VDLT,which discards candidate attendees with high degree to reduce the number of vertices to be considered.The experimental results on real datasets manifest that the proposed algorithms outperform existing approaches on both efficiency and group tenuity. | Na LI Huaijie ZHU Wenhao LU Ningning CUI Wei LIU Jian YIN Jianliang XU Wang-Chien LEE | 2023 | Frontiers of Computer Science2023，17，2： | 0 |
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