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| 1 | Formability,microstructure evolution and mechanical properties of wire arc additively manufactured AZ80M magnesium alloy using gas tungsten arc welding显示文摘Wire arc additive manufacturing(WAAM)technology has been used to fabricate the multi-layer single-pass deposited wall of AZ80M magnesium(Mg)alloy by gas tungsten arc welding.The formability,thermal cycles,microstructural evolution and mechanical properties of the WAAM AZ80M Mg alloy were investigated.The results show that there was significant difference in the temperature variation and the geometries between the original several layers and the subsequent deposited layers.Owing to the arc energy input,the interpass temperature rised rapidly and then stabilized at 150℃.As a result,the width of the deposited wall increased and then kept stable.There were obvious differences in the microstructure of the WAAM AZ80M Mg alloy among the top zone,intermediate zone and bottom zone of deposited wall.During the arc deposition process,theβphase of the WAAM AZ80M Mg alloy redissolved due to the cyclic heat accumulation,and then precipitated in the grain boundary.The cyclic heat accumulation also led to weakening of dendrite segregation.From the substrate to the top zone,the hardness of the deposited wall decreased gradually,and the intermediate zone which was the main body of deposited wall had relatively uniform hardness.The tensile properties of the WAAM AZ80M Mg alloy were different between the vertical direction and the horizontal direction.And the maximum ultimate tensile strength of the WAAM AZ80M Mg alloy was 308 MPa which was close to that of the as-extruded AZ80M Mg alloy. | Yangyang Guo Gaofeng Quan Yinglong Jiang Lingbao Ren Lingling Fan Houhong Pan | 2021 | Journal of Magnesium and Alloys2021，9，1： | 10 |
| 2 | Molecular insights into the heterogeneity of telomere reprogramming in induced pluripotent stem cells显示文摘有各种各样的长度的 telomeres 的返老还童在导致的 pluripotent 干细胞(iPSCs ) 被发现了。telomere 长度规定的机制在 iPSCs 的正式就职和增长期间留下逃犯。我们证明 telomere 动力学在 reprogramming 和经过期间在鼠标 iPSCs 是可变的,并且建议这些差别多半源于多重潜在的因素,包括包括外长的 reprogramming 因素的表示的 telomerase 机械, telomerase 独立的机制和同种细胞的影响。用一个基因模型 telomerase 缺乏(为 iPSCs 的推导和段落的 Terc −/− 和 Terc +/−) 房间,我们发现 telomerase 在 iPSCs 的 reprogramming 和自强起一个关键作用。进一步,当由再结合的延伸和维护的其他的小径也被要求时, telomeres 的 telomerase 维护为真 pluripotency 的正式就职是必要的,然而并非足够。一起, telomere 生物学的几个方面可以在 iPSCs 说明可变 telomere 动力学。尤其是,采用在 iPSC reprogramming 期间维持 telomeres 的机制很类似于胚胎的干细胞的那些。这些调查结果可以也联系到这些机制能为在由体的房间的原子 reprogramming 以后的 telomere 异质负责的克隆的地原子转移。 | Fang Wang Yu Yin Xiaoying Ye Kai Liu Haiying Zhu Lingling Wang Maria Chiourea Maja Okuka Guangzhen Ji Jiameng Dan Bingfeng Zuo Minshu Li Qian Zhang Na Liu Lingyi Chen Xinghua Pan Sarantis Gagos David L Keefe Lin Liu | 2012 | Cell Research2012，22，4： | 7 |
| 3 | Trace bis-(3-sulfopropyl)-disulfide enhanced electrodeposited copper foils显示文摘Smooth and strong copper foils are highly required for the development of printed circuit boards and Li-ion batteries.Adding additives is an efficient and low-cost method to enhance electrodeposited copper foils.Here we chose Bis-(3-sulfopropyl)-disulfide(SPS)as the additive and investigated its concentration on the surface roughness and tensile strength of the electrodeposited copper foils.A copper foil with the smoothest surface(Rz=2.1μm)and the highest tensile strength(~338 MPa)was prepared with1.5 mg/L SPS.The contact angle on the copper foil decreased with the reduction of surface roughness.The enhancing mechanism of the SPS was investigated by scanning electron microscopy and X-ray diffraction.The refined grain and enhanced(220)texture were considered to be the radical reason for the smooth and strong copper foils.The electrochemical results prove the suppressing effect is related to the SPS adsorption. | Lingling Liu Yeqiang Bu Yue Sun Jianfeng Pan Jiabin Liu Jien Ma Lin Qiu Youtong Fang | 2021 | Journal of Materials Science & Technology2021，，15： | 6 |
| 4 | Recombinant adenovirus-p53(Gendicine) sensitizes a pancreatic carcinoma cell line to radiation显示文摘Objective:In this study,we examine the effects of recombinant adenovirus-p53(rAd-p53)on the pancreatic carcinoma cell line SW1990.Specifically,we determine if expression of rAd-p53 sensitizes these cells to radiation.Methods:Following transfection of SW1990 cells with rAd-p53,we measured expression of P53,P21 and Bax by immunocytochemistry.Both transfected and control cell lines were irradiated with a range of doses,and the survival fractions(SF)were calculated.Dose survival curves were constructed and modeled for comparison.Results:Transfection of SW1990 cells with rAd-p53 resulted in increased expression of P53,P21 and Bax in a time-dependent manner.At 96 h after transfection,89.92%of cells expressed P53,56.8%expressed P21,and 76.50%expressed Bax.The SF following radiation was lower in the rAd-p53 transfected cells compared to the control cells,suggesting that rAd-p53 sensitizes SW1990 cells to radiation(D0for the experimental and control groups was 2.199 and 2.462,respectively).Conclusions:Use of the adenoviral vector is an effective means of transfecting SW1990 cells with wild-type P53,and this sensitizes the cell line to irradiation.This work suggests that combining rAd-p53with radiation therapy in pancreatic cancer may be therapeutically beneficial. | Jinluan Li Jianji Pan Xianggao Zhu Ying Su Lingling Bao Sufang Qiu Changyan Zou Yong Cai Junxin Wu Ivan W.K.Tham | 2013 | Chinese Journal of Cancer Research2013，25，6： | 6 |
| 5 | Recycling Isolation of Plant DNA,A Novel Method显示文摘DNA is one of the most basic and essential genetic materials in the field of molecular biology.To date,isolation of sufficient and good-quality DNA is still a challenge for many plant species,though various DNA extraction methods have been published.In the present paper,a recycling DNA extraction method was proposed.The key step of this method was that a single plant tissue sample was recycled for DNA extraction for up to four times,and correspondingly four DNA precipitations(termed as the 1st,2nd,3rd and 4th DNA sample, respectively) were conducted.This recycling step was integrated into the conventional CTAB DNA extraction method to establish a recycling CTAB method.This modified CTAB method was tested in eight plant species,wheat,sorghum,barley,corn,rice,Brachypodium distachyon,Miscanthus sinensis and tung tree.The results showed that high-yield and good-quality DNA samples could be obtained by using this new method in all the eight plant species.The DNA samples were good templates for PCR amplification of both ISSR and SSR markers.The recycling method can be used in multiple plant species and can be integrated with multiple conventional DNA isolation methods,and thus is an effective and universal DNA isolation method. | Lingling Zhang Bo Wang Lei Pan Junhua Peng | 2013 | Journal of Genetics and Genomics2013，40，1： | 5 |
| 6 | Effect of heat treatment on the microstructure and mechanical properties of AZ80M magnesium alloy fabricated by wire arc additive manufacturing显示文摘To maximize the benefits of wire arc additive manufacturing(WAAM)processes,the effect of post-deposition heat treatment on the microstructure and mechanical properties of WAAM AZ80M magnesium(Mg)alloy was investigated.Three different heat treatment procedures(T4,T5 and T6)were performed.According to the results,after T4 heat treatment,the microsegregation of alloying elements was improved with the eutectic structure dissolved.Samples after T5 heat treatment inherited the net-like distribution of secondary phases similar to the as-deposited sample,where the eutectic structure covering the interdendritic regions and theβ-phase precipitated around the eutectic structure.After T6 heat treatment,the tinyβ-phases re-precipitated from the matrix and distributed in inner and outer of the grains.The hardness distribution of the samples went through T4 and T6 heat treatment was more uniform in comparison to that of T5 heat treated samples.The tensile test showed that the T6 heat treatment improved the strength and ductility,and the anisotropy between horizontal and vertical can be eliminated.Moreover,T4 treated samples exhibited highest ductility. | Yangyang Guo Gaofeng Quan Mert Celikin Lingbao Ren Yuhang Zhan Lingling Fan Houhong Pan | 2022 | Journal of Magnesium and Alloys2022，10，7： | 5 |
| 7 | 冷冻干燥更适合土壤活性氮气体(HONO和NO)排放的研究?显示文摘土壤活性氮气体(HONO和NO)排放是全球氮循环的关键过程,对大气化学具有重要意义。本研究采集了农田,草地和森林三种不同土地利用类型的土壤样品,分别对样品进行了风干,冻干和烘干三种处理,并与未处理的新鲜土壤进行比较研究。结果表明,三种类型土壤的冻干土与新鲜土样品的p H值差异最小。总的来说,三种干燥处理都能提高新鲜土壤NH4+-N, NO3--N和溶解态有机N的含量。与新鲜土壤相比,冻干土壤HONO和NO的最大排放通量及完整干湿交替循环的总排放量没有显著增加,而风干和烘干土壤显著增加。因此,冷冻干燥方法可能更适合涉及土壤活性氮气体排放通量的研究。这一发现的重要启示是,我们需要慎重评价以往基于不同干燥方法得到的有关陆地–大气界面活性氮交换过程和生物地球化学氮循环机制的认识。 | WU Dianming DENG Lingling LIU Yanzhuo XI Di ZOU Huilan WANG Ruhai SHA Zhimin PAN Yuepeng HOU Lijun LIU Min | 2020 | Atmospheric and Oceanic Science Letters2020，13，3： | 3 |
| 8 | Recycling Isolation of Plant DNA, A Novel Method显示文摘 | Lingling Zhang Bo Wang Lei Pan Junhua Peng | 2012 | Journal of Genetics and Genomics2012，，： | 2 |
| 9 | Trans-cinnamaldehyde promotes nitric oxide release via the protein kinase-B/v-Akt murine thymoma viral oncogene-endothelial nitric oxide synthase pathway to alleviate hypertension in SHR.Cg-Lepr^(cp)/NDmcr rats显示文摘OBJECTIVE:To evaluate whether endothelial dysfunction and hypertension are prevented by trans-cinnamaldehyde(t CA) through the activation of endothelial nitric oxide synthase(e NOS).METHODS:Human umbilical vein endothelial cells(HUVECs) were cultured in vitro and stimulated with t CA to determine cell viability using the methyl thiazolyl tetrazolium assay.The effect of t CA on nitric oxide(NO) production was determined by diaminofluorescein-dyes in the absence or presence of inhibitors of e NOS,AMPK,PKA,and AKT.The effect of t CA on blood pressure was determined by the tail-cuff method in obesity spontaneous hypertension(SHR.Cg-Leprcp/NDmcr) rats.The phosphorylation of e NOS and protein expression of the insulin-signaling pathway(Ins R-IRS1-PI3 K-AKT)were measured by western blot.RESULTS:t CA at concentrations less than 100 μM did not affect cell viability in cultured HUVECs.Stimulation with t CA promoted NO release in a time-dependent manner compared with the control group.t CA-treated HUVECs also significantly increased AKT-Ser473 and e NOS-Ser1177 phosphorylation.In SHR-CP rats,treatment with t CA at a dose of 40 mg/kg/day for 6 weeks markedly reduced the systolic blood pressure and diastolic blood pressure,increased the phosphorylation of AKT and e NOS,and increased urinary nitric oxidation.CONCLUSION:t CA attenuated endothelial dysfunction and reduced blood pressure in SHR-CP rats.The underlying mechanisms may involve the increase in AKT and e NOS phosphorylation and the release of e NOS-derived NO. | Zhang Lu Wu Lili Liu Ximing Yoshitomi Hisae Ikeda Katsumi Negishi Hiroko Pan Yajing Sun Wen Qin Lingling Li Juan-E Xu Tunhai Liu Tonghua Gao Ming | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 2 |
| 10 | Usefulness of 18 F-FDG PET, combined FDG-PET/CT and EUS in diagnosing primary pancreatic carcinoma: A meta-analysis显示文摘 | Shuang Tang Gang Huang Jianjun Liu Tao Liu Lyndal Treven Saoli Song Chenpeng Zhang Lingling Pan Ting Zhang | 2009 | European Journal of Radiology2009，，1： | 1 |
| 11 | FDG-PET and other imaging modalities for the evaluation of breast cancer recurrence and metastases: a meta-analysis显示文摘 | LingLing Pan Yuan Han XiaoGuang Sun JianJun Liu Huang Gang | 2010 | Journal of Cancer Research and Clinical Oncology2010，，7： | 1 |
| 12 | Seismic evidence for the existence of an entrained mantle flow coupling the northward advancing Indian plate under Tibet显示文摘The Tibetan Plateau, known as 'the roof of the world' and 'the third pole of the earth', is a product of the collision between India and Asia during the last ~50 Ma.The regional tectonics–in particular, growth and expansion of the plateau–has been attributed primarily to deformation within the lithosphere.The role and pattern of the underlying asthenospheric flow, however, remain mostly unaddressed.In light of recent seismic tomographic images and published seismic anisotropic descriptions of the upper mantle, here we propose that an entrained mantle flow is likely to exist under Tibet, induced by the northward advancing Indian plate.The direction of mantle flow is characterized by a gradual rotation from northward in the south to eastward in the north as a result of deflection by the deep root of the Tarim block.The presence of an underlying mantle flow is not only able to account for the west-east oriented fast-axis of seismic anisotropy in northern Tibet, but can also adequately explain the sporadic null splitting in southern Tibet.Specifically, the null splitting results, at least in part, from upwellings of asthenospheric flow through tears of the underthrusting Indian plate that have been revealed by various seismic observations.The mantle flow may in turn promote the block extrusion under Tibet that has been observed in GPS measurements; hot asthenospheric upwellings may also lead to widespread post-collisional magmatism in southern Tibet. | Yu Zou XiaoBo Tian YouQiang Yu Fa-Bin Pan LingLing Wang XiaoBo He | 2019 | Earth and Planetary Physics2019，3，1： | 1 |
| 13 | Glimepiride induces proliferation and differentiation of rat osteoblasts via the PI3-kinase/Akt pathway显示文摘 | Pan Ma Bin Gu Junli Ma Lingling E Xia Wu Junkai Cao Hongchen Liu | 2010 | Metabolism2010，，3： | 1 |
| 14 | Zn (11) - PEG 300 globules as soft template for the synthesis of hexagonal ZnO micro- nuts by the hydrothermal reaction method 显示文摘 | SHI XIXI PAN LINGLING CHEN SHUOPING | 2009 | Langmuir2009，25，10： | 1 |
| 15 | Prognostic significance of SUV on PET/CT in patients with esophageal cancer: a systematic review and meta-analysis显示文摘 | LingLing Pan Ping Gu Gang Huang HuiPing Xue ShuQi Wu | 2009 | European Journal of Gastroenterology & Hepatology2009，，9： | 1 |
| 16 | Lightweight structure of engine hood显示文摘 | Wang Hongyan Gao Weimin Pan Lingling | 2006 | Journal of Tongji University:Natural Science2006，34，8： | 1 |
| 17 | Inhibition of cytoplasmic GSK-3β increases cisplatin resistance through activation of Wnt/β-catenin signaling in A549/DDP cells显示文摘 | Yuan Gao Zan Liu Xiuwei Zhang Jiani He Yongqi Pan Fengxia Hao Lingling Xie Qingchang Li Xueshan Qiu Enhua Wang | 2013 | Cancer Letters2013，，1： | 1 |
| 18 | The SU- Vmax(maximum standardized uptake value for F-18 fluo- rodeoxyglucose) and serum squamous cell carcinoma anti- gen(SCC-ag) function as prognostic biomarkers in patients with primary cervical cancer显示文摘 | Lingling Pan Jingyi Cheng Min Zhou | 2012 | Journal of Cancer Research and Clinical Oncology2012，，2： | 1 |
| 19 | Mailuoning suppresses H_2O_2-induced cortical neuronal injury and correlates with increased catalase activity and inhibited oxidative stress function显示文摘BACKGROUND:Mailuoning,a Chinese herb,has been widely used in China to treat acute ischemic stroke,and the major component exhibits anti-oxidative effects.However,the precise anti-oxidation pathway remains uncertain.OBJECTIVE:To validate the protective effects of Mailuoning on H2O2-induced primary cortical neuron injury in embryonic mice.DESIGN,TIME AND SETTING:Comparative observation and in vitro experiments were performed at the Jiangsu Key Laboratory for Molecular Medicine from January 2008 to September 2009.MATERIALS:Mailuoning(Nanjing Jinling Medical Company,China),reactive oxygen species(ROS) kit(Beyotime Biotechnology,China),superoxide dismutase(SOD),Cu/Zn SOD kit,malondialdehyde(MDA) kits(Nanjing Jiancheng,China),mitochondrial membrane potential(GMS10013.1,GENMED,USA) and catalase activity assay kit(Beyotime Biotechnology,China) were utilized for the present study.METHODS:Mouse embryonic cortical neurons were isolated and cultured with culture medium containing H2O2(80 μmol/L) and/or Mailuoning(1.25 μg/mL) for 24 hours.MAIN OUTCOME MEASURES:Neuronal viability and death were detected by methyl thiazolyl tetrazdium and flow cytometry;ROS production was determined by flow cytometry;mitochondrial membrane potential was detected using fluorescent staining;SOD activity was detected using a modified nitroblue tetrazolium method;Cu/Zn SOD and catalase activity was detected by spectrophotometry;and MDA was determined using the lipid peroxidation method.RESULTS:H2O2 increased ROS production and MDA concentration(P < 0.05),and decreased mitochondrial membrane potential,SOD,Cu/Zn SOD and catalase activity(P < 0.05);the number of surviving neurons(P < 0.05) was also reduced.Mailuoning reversed these changes.CONCLUSION:Mailuoning protects H2O2-induced injury in cortical cells by inhibiting ROS and MDA,increasing depolarization of mitochondrial membrane,and enhancing SOD and catalase activity. | Xiaofan Pan Siyuan Huang Lingling Li Yun Xu | 2010 | Neural Regeneration Research2010，5，8： | 1 |
| 20 | Eosinophils promote pulmonary matrix destruction and emphvsema via Cathepsin L显示文摘Patients with chronic obstructive pulmonary disease(COPD)who exhibit elevated blood eosinophil levels often experience worsened lung function and more severe emphysema.This implies the potential involvement of eosinophils in the development of emphysema.However,the precise mechanisms underlying the development of eosinophil-mediated emphysema remain unclear.In this study,we employed single-cell RNA sequencing to identify eosinophil subgroups in mouse models of asthma and emphysema,followed by functional analyses of these subgroups.Assessment of accumulated eosinophils unveiled distinct transcriptomes in the lungs of mice with elastase-induced emphysema and ovalbumin-induced asthma.Depletion of eosinophils through the use of anti-interleukin-5 antibodies ameliorated elastase-induced emphysema.A particularly noteworthy discovery is that eosinophil-derived cathepsin L contributed to the degradation of the extracellular matrix,thereby leading to emphysema in pulmonary tissue Inhibition of cathepsin L resulted in a reduction of elastase-induced emphysema in a mouse model.Importantly,eosinophil levels correlated positively with serum cathepsin L levels,which were higher in emphysema patients than those without emphysema.Expression of cathepsin L in eosinophils demonstrated a direct association with lung emphysema in COPD patients.Collectively,these findings underscore the significant role of eosinophil-derived cathepsin L in extracellular matrix degradation and remodeling,and its relevance to emphysema in coPD patients.Consequently,targeting eosinophil-derived cathepsin L could potentially offer a therapeutic avenue for emphysema patients.Further investigations are warranted to explore therapeutic strategies targeting cathepsin L in emphysema patients. | Xia Xu Tao Yu Lingling Dong Rainer Glauben Siyuan Wu Ronghua Huang Shiwei Qumu Chenli Chang Jing Guo Lin Pan Ting Yang Xin Lin Ke Huang Zhihua Chen Chen Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 1 |
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