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| 1 | Lamin-like Proteins Negatively Regulate Plant Immunity through NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 and NONEXPRESSOR OF PR 3ENES1 in Arabidopsis thaliana显示文摘 | Tongtong Guo Xuegao Mao Hui Zhang Yu Zhang Mengdi Fu Zhenfei Sun Peng Kuai Yonggen Lou Yuda Fang | 2017 | Molecular Plant2017，10，10： | 3 |
| 2 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 3 | In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica显示文摘 | Zhenfei Peng Min Liu Zhexiang Fang Qiqing Zhang | 2012 | International Journal of Biological Macromolecules2012，，5： | 1 |
| 4 | In vitro antiprolifeative effect of a water-soluble Laminaria japonica polysaccharide on human melanoma cell line A375显示文摘 | Zhenfei PENG Min LIU Zhexiang FANG | 2013 | Food and Chemical Toxicology2013，58，： | 1 |
| 5 | Composition and cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica)显示文摘 | Peng Zhenfei Liu Min Fang Zhexiang | 2012 | Carbohydrate Polymers2012，89，4： | 1 |
| 6 | In vitro selection of salinity tolerant variants from triploid bermudagrass (Cynodon transvaalensis?×?C. dactylon) and their physiological responses to salt and drought stress显示文摘 | Shaoyun Lu Xinxiang Peng Zhenfei Guo Gengyun Zhang Zhongcheng Wang Congying Wang Chaoshu Pang Zhen Fan Jihua Wang | 2007 | Plant Cell Reports2007，，8： | 1 |
| 7 | in vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica显示文摘 | PENG Zhenfei LIU Min FANG Zhexiang | 2012 | Int J Biol Macromol2012，50，： | 1 |
| 8 | Inactivated SARS-CoV-2 induces acute respiratory distress syndrome in human A CE2-transgenic mice显示文摘The development of animal models for COVID-19 is essential for basic research and drug/vaccine screening.Previously reported COVID-19 animal models need to be established under a high biosafety level condition for the utilization of live SARS-CoV-2,which greatly limits its application in routine research.Here,we gen erate a mouse model of COVID-19 un der a gen eral laboratory condition that captures multiple characteristics of SARS-CoV-2-induced acute respiratory distress syndrome(ARDS)observed in huma ns.Briefly,human ACE2-tra nsge nic(MCE2)mice were in tratracheally in stilled with the formaldehyde-inactivated SARS-CoV-2,resulting in a rapid weight loss and detrimental changes in lung structure and function.The pulmonary pathologic changes were characterized by diffuse alveolar damage with pulmonary consolidation,hemorrhage,necrotic debris,and hyaline membrane formation.The production of fatal cytokines(IL-β,TNF-α,and IL-6)and the infiltration of activated neutrophils,inflammatory monocyte-macrophages,and T cells in the lung were also determined,suggesting the activation of an adaptive immune response.Therapeutic strategies,such as dexamethasone or passive antibody therapy,could effectively ameliorate the disease progression in this model.Therefore,the established mouse model for SARS-CoV-2-induced ARDS in the current study may provide a robust tool for researchers in the standard open laboratory to investigate the pathological mechanisms or develop new therapeutic strategies for COVID-19 and ARDS. | Zhenfei Bi Weiqi Hong Haiying Que Cai He Wenyan Ren Jingyun Yang Tianqi Lu Li Chen Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 9 | A human monoclonal antibody neutralizes SARS-CoV-2 Omicron variants bytargeting the upstream region of spikeprotein HR2 motif显示文摘The continuous emergence of new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants meansthere is a need to explore additional strategies to develop broad-spectrum vaccines or therapeutics for individuals remaining at risk of coronavirus disease 2019(COVID-19).Neutralizing monoclonal antibody(mAb)that binds to theconserved S2 subunit of the SARS-CoV-2 spike(S)protein alone,or in combination with mAb that binds to the receptor-binding domain(RBD)of S protein,might be effective in eliciting protection from infection by a variety of SARS-CoV2 variants.Using high-throughput single-cell immunoglobulin sequencing of B cells from COVID-19-convalescent donors,we identified a high-affinity S2-specific mAb-39,that could inhibit original SARS-CoV-2 strain,Omicron BA.1,BA.2.86,BA.4,BA.5,and EG.5.1 S protein-mediated membrane fusion,leading to the neutralization of these pseudoviralinfections.Moreover,mAb-39 could also improve the neutralizing activity of anti-RBD antibody against the highlyneutralization-resistant Omicron variants.Molecular docking and point mutation analyses revealed that mAb-39 recognized epitopes within the conserved upstream region of the heptad repeat 2(HR2)motif of the S2 subunit.Collectively,these findings demonstrate that targeting the conserved upstream region of the HR2 motif(e.g.,using mAbs)provides anovel strategy for preventing the infection of SARS-CoV-2 and its variants. | Hang Su Jun Zhang Zhenfei Yi Sajid Khan Mian Peng Liang Ye Alan Bao Han Zhang Guangli Suo Qian Li Housheng Zheng Dandan Wu Thomas J.Kipps Lanfeng Wang Zhenghong Lin Suping Zhang | 2024 | hLife2024，2，3： | 0 |
| 10 | A mouse model for SARS-CoV-2-induced acute respiratory distress syndrome显示文摘Dear Editor,The COVID-19 pandemic has covered more than 200 countries and regions around the world since its outbreak in January 2020.To date,the SARS-CoV-2 virus has caused>1.2 million deaths.The mortality rate of COVID-19 is closely concerned with the clinical symptoms of the patients from mild-to-severe disease.Notably,in its most severe form,COVID-19 leads to life-threatening pneumonia and acute respiratory distress syndrome(ARDS),which is mostly accom-panied by a hyperactive immune response called'cytokine storm'and has high death rates from 40 to 50%. | Weiqi Hong Jingyun Yang Zhenfei Bi Cai He Hong Lei Wenhai Yu Yun Yang Changfa Fan Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
| 11 | Clinical Signifi cance of Angiographically Detectable Neovascularity in Patients with Cardiac Myxoma显示文摘Background:Myxomas are the most common primary cardiac tumors.Angiographically detectable neovascularity(ADN)of myxoma is increasingly being reported as a result of the use of coronary angiography(CAG)to detect coronary artery disease.However,the clinical signifi cance of these fi ndings is not fully understood.Methods:We enrolled 59 patients with cardiac myxoma who also underwent CAG between January 2013 and October 2018.Patients were followed up for a mean of 28.9 months(range 1-69 months).The clinical features,echocardiography measurements,pathological examination fi ndings,CAG results,and outcomes during follow-up were compared between patients with ADN and patients without ADN.Results:ADN was found in 25 patients(42.4%).The arteries feeding the ADN included the right coronary artery(n=15),the left circumfl ex coronary artery(n=7),and both arteries(n=3).The patients with ADN had a higher proportion of eosinophils(3.2%vs.2.2%,P=0.03)and higher low-density lipoprotein cholesterol level(2.7 mmol/L vs.2.2 mmol/L,P=0.02).Myxoma pedicles were more likely to be located in the interatrial septum in patients with ADN(96%vs.73.5%,P=0.02).No signifi cant correlation was observed between the groups in clinical manifestations,atrial arrhythmia,myxoma size,cardiac chamber size,left ventricular ejection fraction,and the prevalence of complication with coronary artery disease[16%in the ADN group(n=4)vs.20.6%in the non-ADN group(n=7),P=0.66].However,patients with ADN tended to have a lower incidence of major adverse cardiac and cerebrovascular events on long-term follow-up(0%vs.14.7%,P=0.07).Conclusion:CAG-detected ADN in patients with cardiac myxoma is associated with a borderline lower rate of major adverse cardiac and cerebrovascular events. | Xiaofan Peng Yichao Xiao Yanan Guo Zhaowei Zhu Liyan Liao Xiaobo Liao Xinqun Hu Zhenfei Fang Xuping Li Shenghua Zhou | 2021 | Cardiovascular Innovations and Applications2021，，4： | 0 |
| 12 | A Giant Right Atrial Myxoma with Blood Supply from the Left and Right Coronary Arteries:Once in a Blue Moon显示文摘Cardiac myxomas,the commonest primary benign cardiac tumors,are extremely rare,with an incidence ranging from 0.0017 to 0.19%and only about one-fi fth of them originating from the right chambers of the heart.A 60-year-old woman was admitted because of recurrent attacks of chest tightness and shortness of breath.Transthoracic echocardiography detected a giant mass in the right atrium;myxoma was indicated by[18 F]fl uorodeoxyglucose PET/CT.Preoperative selective coronary angiography was performed to assess the extent and severity of coronary stenosis,and showed a strongly neovascularized right atrial mass supplied by two feeding vessels with multiple branches from the left and right coronary arteries.The myxoma was successfully excised with open heart surgery and the patient was free of myxoma recurrence during the 3-year follow-up. | Yichao Xiao Zhenfei Fang Xinqun Hu Qiming Liu Zhaowei Zhu Na Liu Xiaofan Peng Shenghua Zhou | 2020 | Cardiovascular Innovations and Applications2020，，1： | 0 |
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