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| 1 | Laparoscopic Partial Nephrectomy With Segmental Renal Artery Clamping:Technique and Clinical Outcomes显示文摘Background:Warm ischemic injury is one of the most important factors affecting renal function in partial nephrectomy(PN).The technique of segmental renal artery clamping emerges as an alternative to conventional renal artery clamping for renal hilar control.Objective:To evaluate the feasibility and efficiency of laparoscopic PN(LPN) with segmental renal artery clamping in comparison with the conventional technique.Design,setting,and participants:A total of 75 patients underwent LPN from June 2007 to November 2009.All patients had T1a or T1b tumor in one kidney and a normal contralateral kidney.Thirty-seven patients underwent surgeries with main renal artery clamping,and 38 underwent surgeries with segmental artery clamping.Intervention:All procedures were performed by the same laparoscopic surgeon.Measurements:Blood loss,operation time,warm ischemia(WI) time,and complications affected renal function before and after operation were recorded.Results and limitations:All LPNs were completed without conversion to open surgery or nephrectomy.The novel technique slightly increased WI time(p < 0.001) and intraoperative blood loss(p = 0.006),while it provided better postoperative affected renal function(p < 0.001) compared with the conventional technique.The total complication rate was 12%.Among the 38 cases where segmental renal artery clamping was performed,7 had to convert to the conventional method.Tumor size and location influenced the number of clamped segmental arteries.Long-term postoperative renal function is still awaited.Conclusions:LPN with segmental artery clamping is safe and feasible in clinical practice.It minimizes the intraoperative WI injury and improves early postoperative affected renal function compared with main renal artery clamping.(C) 2010 European Association of Urology.Published by Elsevier B.V.All rights reserved. | Shao,P.F. Qin,C. Yin,C.J. Meng,X.X. Ju,X.B. Li,J. Lü,Q.A. Zhang,W. Xu,Z.Q.Shao,Pengfei Qin,Chao Yin,Changjun Meng,Xiaoxin Ju,Xiaobing Li,Jie Lü,Qiang Zhang,Wei Xu,Zhengquan] Nanjing Med Univ,Affiliated Hosp 1,Dept Urol,Nanjing,Peoples R China.Guangzhou Rd 300,Nanjing 210029,Peoples R China. | 2011 | 南京医科大学学报(自然科学版)2011，31，7： | 123 |
| 2 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 3 | Heavy metal pollution in Tianjin Bohai Bay,China显示文摘The contamination levels and distribution characters of heavy metals in coastal waters and sediments from Tianjin Bohai Bay,China were examined.Pb and Zn were found as the main heavy metal pollutants in the coastal waters of the bay.High levels of Pb and Zn appeared especially near the estuary,indicating that river discharge was the main pollution source.Moreover,atmospheric deposition resulted in Pb contamination in the middle of the embayment.Analysis of data for the period 1987-2004 indicated that Pb ... | MENG Wei QIN Yanwen ZHENG Binghui | 2008 | Journal of Environmental Sciences2008，20，7： | 41 |
| 4 | Evidence chain-based causality identification in herb-induced liver injury: exemplification of a well-known liver-restorative herb Polygonum multiflorum显示文摘草药的药最近在美国作为导致药的肝损害(DILI ) 的第二个很普通的原因被认出了。然而,识别一些植物的 DILI 诱发性的可靠方法例如 Heshouwu (蓼 multiflorum 的弄干的根) ,留下缺乏。在这研究,有肝机能障碍和 Heshouwu DILI 的 147 个报导文学的案例的 12 307 个住院病人的一个总数被屏蔽。一个一般算法仅仅显示了那 22.5%(9/40 ) 并且(45/147 ) 分别地, 30.6% 所有住院和文学案例报告表明 Heshouwu 的 DILI 诱发性的高概率。由对比,(19/20 )95% 生药学,植物化学,和 metabolomic 有希望地调查的所有情况测试展出高度可能的诱发性,包括不正确地以前被归因的一个病人和从由 pharmacognostic 证据的 Heshouwu 诱发性被排除的一个案例。污染也是的毒素(重金属,杀虫剂,和 mycotoxins ) 从 Heshouwu DILI 诱发性排除了。为 Heshouwu DILI 诊断地址安全的这些屏蔽方法的客观性担心考虑包含芪的草药的药和饮食的补充。 | Jiabo Wang Zhijie Ma Ming Niu Yun Zhu Qingsheng Liang Yanling Zhao Jingyuan Song Zhaofang Bai Yaming Zhang Ping Zhang Na Li Yakun Meng Qi Li Lushan Qin Guangju Teng Junling Cao Baosen Li Shilin Chen Yonggang Li Zhengsheng Zou Honghao Zhou Xiaohe Xiao | 2015 | Frontiers of Medicine2015，9，4： | 47 |
| 5 | Clean Coal Technologies in China: Current Status and Future Perspectives显示文摘 | Shiyan Chang Jiankun Zhuo Shuo Meng Shiyue Qin Qiang Yao | 2016 | Engineering2016，2，4： | 43 |
| 6 | Cold adaptation in pigs depends on UCP3 in beige adipocytes显示文摘 | Jun Lin Chunwei Cao Cong Tao Rongcai Ye Meng Dong Qiantao Zheng Chao Wang Xiaoxiao Jiang Guosong Qin Changguo Yan Kui Li John R. Speakman Yanfang Wang Wanzhu Jin Jianguo Zhao | 2017 | Journal of Molecular Cell Biology2017，9，5： | 20 |
| 7 | Thiolactone copolymer donor gifts organic solar cells a 16.72% efficiency显示文摘Since 1995,bulk-heterojunction organic solar cells consisting of one or two organic donors and one or two organic acceptors have been fighting for high power conversion efficiencies(PCEs)and good stability[1].Until recent years,this next-generation photovoltaic technology starts to offer decent PCEs,shedding the light on commercialization,and attracting great attention again[2-14].Compared w让h the continuously emerging highperformance nonfullerene acceptors,high-performance donors are rare. | Ji Xiong Ke Jin Yufan Jiang Jianqiang Qin Tan Wang Jinfeng Liu Qishi Liu Haili Peng Xiongfeng Li Anxin Sun Xianyi Meng Lixiu Zhang Ling Liu Wenting Li Zhimin Fang Xue Jia Zuo Xiao Yaqing Feng Xiaotao Zhang Kuan Sun Shangfeng Yang Shengwei Shi Liming Ding | 2019 | Science Bulletin2019，64，21： | 17 |
| 8 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 9 | Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy显示文摘Bone marrow mesenchymal stem cells can differentiate into neurons and astrocytes after transplantation in the spinal cord of rats with ischemia/reperfusion injury. Although bone marrow mesenchymal stem cells are known to protect against spinal cord ischemia/reperfusion injury through anti-apoptotic effects, the precise mechanisms remain unclear. In the present study, bone marrow mesenchymal stem cells were cultured and proliferated, then transplanted into rats with ischemia/reperfusion injury via retro-orbital injection. Immunohistochemistry and immunofluorescence with subsequent quantification revealed that the expression of the axonal regeneration marker, growth associated protein-43, and the neuronal marker, microtubule-associated protein 2, significantly increased in rats with bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Furthermore, the expression of the autophagy marker, microtubule-associated protein light chain 3B, and Beclin 1, was significantly reduced in rats with the bone marrow mesenchymal stem cell transplantation compared with those in rats with spinal cord ischemia/reperfusion injury. Western blot analysis showed that the expression of growth associated protein-43 and neurofilament-H increased but light chain 3B and Beclin 1 decreased in rats with the bone marrow mesenchymal stem cell transplantation. Our results therefore suggest that bone marrow mesenchymal stem cell transplantation promotes neurite growth and regeneration and prevents autophagy. These responses may likely be mechanisms underlying the protective effect of bone marrow mesenchymal stem cells against spinal cord ischemia/reperfusion injury. | Fei Yin Chunyang Meng Rifeng Lu Lei Li Ying Zhang Hao Chen Yonggang Qin Li Guo | 2014 | Neural Regeneration Research2014，9，18： | 16 |
| 10 | Targets and molecular mechanisms of triptolide in cancer therapy显示文摘Triptolide(TPL/TL) is a natural drug with novel anticancer effects. Preclinical studies indicated that TPL inhibits cell proliferation, induces cell apoptosis, inhibits tumor metastasis and enhances the effect of other therapeutic methods in various cancer cell lines. Multiple molecules and signaling pathways, such as caspases, heat-shock proteins, NF-κB, and deoxyribonucleic acid(DNA) repair-associated factors, are associated with the anti-cancer effect. TPL also improves chemoradiosensitivity in cancer therapy. Phase I trials indicate the potential clinical value of TPL use. However, further trials with larger sample sizes are needed to confirm these results. | Cuicui Meng Hongcheng Zhu Hongmei Song Zhongming Wang Guanhong Huang Defan Li Zhaoming Ma Jianhua Ma Qin Qin Xinchen Sun Jianxin Ma | 2014 | Chinese Journal of Cancer Research2014，26，5： | 16 |
| 11 | Thorombolytic therapy with rescue percutaneous coronary intervention versus primary percutaneous coronary intervention in patients with acute myocardial infarction: a multicenter randomized clinical trial显示文摘背景尽管有营救的 thrombolytic 治疗经皮的冠的干预(一种总线标准)是为圣片断举起的普通治疗策略尖锐心肌的梗塞( STEMI ),欠缺的数据在它相对主要一种总线标准,和比较的功效上是可得到的因此是这 study.Methods 的目的这 multicenter ,开标签,使随机化,平行试用与 STEMI 在病人(年龄 70 年)上在 12 所医院里被进行在 12 以内介绍了症状发作的小时(吝啬的间隔> 3 个小时)。病人们被使随机化到三个组:主要一种总线标准组(n=101 ) ;recombinant staphylokinase (r-Sak ) 组(n=104 ) ;并且 recombinant 织物类型 plasminogen 使活跃之物(rt-PA ) 组(n=106 ) 。为分配到 thrombolytic 治疗手臂的所有病人,冠的 angiography 在药治疗以后在 90 分钟被执行证实梗塞相关的动脉(IRA ) 明显;营救一种总线标准与 TIMI 流动等级 2 在情况中被执行。赤裸金属的 stent 培植为所有病人被计划。 113.4 的一般水准纪录开始 thrombolytic 治疗( door-to-needle 时间)和 141.2 分钟在 IRA (到汽球时间的门)执行第一汽球通货膨胀的在它要求了的随机化以后的结果 IRA 明显( TIMI 流动等级 2 或 3 )和 TIMI 流动等级的 .Rates 3 是显著地更低的在在 90 点的 thrombolysis 组在过程的结束比在主要一种总线标准组在药治疗以后纪录(70.5%对98.0%, P < 0.0001 ,和53.0%对85.9%, P < 0.0001 ,分别地)。有 stenting 的营救一种总线标准在 thrombolytic 治疗手臂在 117 个病人(55.7%) 被执行。明显和 3 仍然是的 TIMI 流动等级的率显著地比在主要一种总线标准组在营救一种总线标准降低(88.9% 对 97.9% , P=0.0222,并且 68.4% 对 85.0% , P=0.0190,分别地) 。在 30 天治疗以后,死亡率在比在主要一种总线标准组与营救一种总线标准组相结合的 thrombolysis 是显著地更高的(7.1% 对 0, P=0.0034 ) 。 death/Ml 和流血复杂并发症的率比在主要一种总线标准组在与营救一种总线标准组一起的 thrombolysis 是显著地更高的(10.0%对1.0%, P=0.0380 ,并且28.10%对8.91%, P=0.0001 ,分别地)有营救一种总线标准的 .Conclusions Thrombolytic 治疗与冠的明显和 TIMI 流动等级的显著地更低的率被联系 3 ,但是与在 30 天的死亡, death/Ml 和出血性的复杂并发症的显著地更高的率,作为与在这组中国 S | GAO Run-lin HAN Ya-ling YANG Xin-chun MAO Jie-ming FANG Wei-yi WANG Lei SHEN Wei-feng LI Zhan-quan JIA Guo-liang LU Shu-zheng WEI Meng ZENG Ding-yin CHEN Ji-lin QIN Xue-wen XU Bo DU Chang-hui | 2010 | Chinese Medical Journal2010，，11： | 15 |
| 12 | Clinical significance of multislice spiral CT scans in hepatic veins occlusion in Budd-Chiari syndrome显示文摘有肝的静脉吸藏(HVBCS ) 的背景 Budd-Chiari 症候群能导致严重的门高血压和肝损坏。我们回顾地分析了 HVBCS 的肝的 CT 特征并且在它的 diagnosis.Methods 评估了 CT 考试和 CT angiography (CTA ) 的 triphasic 改进的实用性有 HVBCS 的 25 个盒子,由数字减法 angiography (DSA ) 证实了,在 DSA 前在一个星期以内收到了 triphasic 改进 CT 扫描。相关的血容器的 CTA 图象与最大的紧张设计被重建,显示的体积并且倾斜与 DSA ,横向的 CT 的察觉率和 CTA 图象相比重新格式化 techniques.Results 因为反常肝的静脉是81.7%( 58/71 )并且95.8%( 68/71 )( 2=7.044 , P=0.008 ),为膜的阻塞是47.4%( 9/19 )并且84.2%( 16/19 )分别地( 2 =5.729 , P=0.017 )因为部分阻塞是88.0%( 22/25 )并且100%( 25/25 )分别地( 2=1.418 , P=0.234 )。为肝的静脉狭窄的察觉率是有每个方法的 100% 。弥漫的肝肿大在尖锐阶段在所有 6 个盒子中被发现并且在长期的阶段的 19 个盒子中的 3 个没有专利 intrahepatic 担保,有三根肝的静脉的严重阻塞。在长期的阶段的另外的 16 个盒子有 hepatatrophia 到与妨碍的肝的静脉有关的不同程度。都在尖锐阶段并且 15 开始在有尾的脑叶和 perihilar 区域在长期的阶段介绍了典型补缀的改进并且与时间扩大了延期。在所有情况中,有萎缩,坏死和拥挤的实质区域表明了更低、以后的改进。在所有部分,它有正常改进,至少一获流出专利肝的静脉,附件肝的静脉或并行的容器被检测。由 multislice 螺线 CT 的结论动态改进 CT 检查能不仅由 CTA 技术改进 HVBCS 的诊断,而且能 noninvasively 提供解剖信息并且揭示损坏到肝的实质。 | MENG Xiao-chun ZHU Kang-shun QIN Jie ZHANG Jian-sheng WANG Xiao-hong ZOU Yan ZHANG Ya-qin SHAN Hong | 2007 | Chinese Medical Journal2007，，2： | 15 |
| 13 | Teleportation of an arbitrary two-qudit state based on the non-maximally four-qudit cluster state显示文摘Two different schemes are presented for quantum teleportation of an arbitrary two-qudit state using a non-maximally four-qudit cluster state as the quantum channel. The first scheme is based on the Bell-basis measurements and the re-ceiver may probabilistically reconstruct the original state by performing proper transformation on her particles and an auxiliary two-level particle; the second scheme is based on the generalized Bell-basis measurements and the probability of successfully teleporting the unknown state depends on those measurements which are adjusted by Alice. A comparison of the two schemes shows that the latter has a smaller probability than that of the former and contrary to the former, the channel information and auxiliary qubit are not necessary for the receiver in the latter. | TIAN DongPing TAO YingJuan QIN Meng | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，10： | 14 |
| 14 | Differences in HER2 over-expression between proximal and distal gastric cancers in the Chinese population显示文摘AIM: To investigate HER2 expression and its correlation with clinicopathological variables between proximal and distal gastric cancers (GC) in the Chinese population. METHODS: Immunostaining of HER2 was performed and scored on a scale of 0-3 in 957 consecutive GC cases, according to the revised scoring criteria of HercepTest TM as used in the ToGA trial. Correlations between HER2 expression and clinicopathologic variables of proximal (n = 513) and distal (n = 444) GC were investigated. RESULTS: Our results showed that HER2 expression was significantly higher in the proximal than in distal GC (P < 0.05). Overall, HER2 expression was significantly higher in male patients (P < 0.01), the Lauren intestinal type (P < 0.001), low-grade (P < 0.001) and pM1 (P < 0.01) diseases, respectively. There was a significant difference in HER2 expression among some pTNM stages (P < 0.05). In contrast, HER2 expression in the distal GC was significantly higher in male patients (P < 0.001), low-grade histology (P < 0.001), the Lauren intestinal type(P < 0.001), and pM1 (P < 0.001). In the proximal GC, however, higher HER2 expression scores were observed only in tumors with low-grade histology (P < 0.001) and the Lauren intestinal type (P < 0.001). CONCLUSION: HER2 over-expression in GC of Chinese patients was significantly more common in proximal than in distal GC, and significantly correlated with the Lauren intestinal type and low-grade histology in both proximal and distal GC, and with pM1 disease and male gender in distal GC. | Xiang-Shan Fan Jie-Yu Chen Chang-Feng Li Yi-Fen Zhang Fan-Qing Meng Hong-Yan Wu An-Ning Feng Qin Huang | 2013 | World Journal of Gastroenterology2013，19，21： | 13 |
| 15 | Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M显示文摘In this work,rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks,a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence,with a stable and high productive mutant strain,it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale. | ZHU Yong GAN Jun-jiang ZHANG Guo-liang YAO Bin ZHU Wen-jie MENG Qin | 2007 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2007，8，9： | 12 |
| 16 | Use of First-phase Insulin Secretion in Early Diagnosis of Thyroid Diabetes and Type 2 Diabetes Mellitus显示文摘 | Li-Heng Meng Yao Huang Jia Zhou Xing-Huan Liang Jing Xian Li Li Ying-Fen Qin | 2017 | Chinese Medical Journal2017，，7： | 12 |
| 17 | Seven-day triple therapy is a better choice for Helicobacter pylori eradication in regions with low antibiotic resistance显示文摘AIM: To investigate whether 7-d triple therapies are still valid in populations with low levels of resistance.METHODS: A total of 1106 Helicobacter pylori(H. pylori)-positive patients were divided into three groups,each of which received one type of 7-d triple therapy. Therapeutic outcomes of the patients were assessed by the 13C-urea breath test at 8 wk after treatment. The susceptibility of H. pylori to antibiotics was determined by an agar-dilution method. Data analysis was performed by χ2 tests.RESULTS: The eradication rates in groups A,B and C were 90.71%(332/366),90.46%(313/346) and 90.87%(189/208),respectively(P = 0.986). The resistance rates were 8.91% for clarithromycin,14.78% for levofloxacin and 0% for amoxicillin. The eradication rate was significantly different between clarithromycin-and levofloxacin-resistant patients(P < 0.05) in group A. Patients whose treatment failed in group A also had a higher clarithromycin resistance rate than did successive patients(P = 0.034). However,levofloxacin resistance had no obvious influence on the eradication rate. Furthermore,three main antibiotics(clarithromycin,levofloxacin and amoxicillin) had lower DID(defined daily dose per 1000 inhabitants per day) in this city.CONCLUSION: Clarithromycin resistance is the main reason for the failure of 7-d triple therapy. In populations with low levels of resistance,a 7-d triple therapy is a viable choice. The choice of therapy should not be influenced by conditions in high antibiotic resistance regions. | Yue-Feng Tong Jun Lv Li-Yuan Ying Fang Xu Bo Qin Ming-Tong Chen Fei Meng Miao-Ying Tu Ning-Min Yang You-Ming Li Jian-Zhong Zhang | 2015 | World Journal of Gastroenterology2015，21，46： | 12 |
| 18 | MicroRNA-34a promoting apoptosis of human lens epithelial cells through down-regulation of B-cell lymphoma-2 and silent information regulator显示文摘·AIM: To investigate the role of micro RNA-34a(mi R-34a) in the induction of apoptosis of human lens epithelial(HLE-B3) cells.· METHODS: The apoptosis of HLE-B3 cells was detected by Annexin V-PE apoptosis detection kit after the treatment with 200 μmol/L H2O2 for 24h and lentiviral mi R-34 a vector transfection. The expression of mi R-34 a in the cells was quantified by quantitative real time polymerase chain reaction(q RT-PCR) in response to H2O2 exposure and the vector transfection. The effects of overexpression of mi R-34 a on the expression of B-cell lymphoma-2(Bcl-2) and silent information regulator 1(SIRT1) was determined by q RT-PCR and Western blot.· RESULTS: The expression of mi R-34 a was up-regulated by the treatment of H2O2 in HLE-B3 cells. The increased expression of mi R-34 a is accompanied with the cell apoptosis. Consistence with the H2O2 exposure,ectopic overexpression of mi R-34 a in HLE-B3 cells promoted cells apoptosis. Importantly the anti-apoptosis factors Bcl-2 and SIRT1 were reduced significantly by up-regulation of mi R-34 a in HLE-B3 cells.·CONCLUSION: Mi R-34 a promotes the apoptosis of HLE-B3 cells by down-regulating Bcl-2 and SIRT1,suggesting that mi R-34 a may involve in the pathogenesis of cataract formation and targeting mi R-34 a may be a potentially therapeutic approach for treatment of cataract. | Qing-Lan Li Hong-Yang Zhang Yong-Jie Qin Qian-Li Meng Xiao-Lei Yao Hai-Ke Guo | 2016 | International Journal of Ophthalmology(English edition)2016，9，11： | 11 |
| 19 | Intestinal lysozyme liberates Nod1 ligands from microbes to direct insulin trafficking in pancreatic beta cells显示文摘Long-range communication between intestinal symbiotic bacteria and extra-intestinal organs can occur through circulating bacterial signal molecules, through neural circuits, or through cytokines or hormones from host cells. Here we report that Nod1 ligands derived from intestinal bacteria act as signal molecules and directly modulate insulin trafficking in pancreatic beta cells. The cytosolic peptidoglycan receptor Nod1 and its downstream adapter Rip2 are required for insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, Nod1 and Rip2 localize to insulin vesicles, recruiting Rab1a to direct insulin trafficking through the cytoplasm. Importantly, intestinal lysozyme liberates Nod1 ligands into the circulation, thus enabling long-range communication between intestinal microbes and islets. The intestine-islet crosstalk bridged by Nod1 ligands modulates host glucose tolerance. Our study defines a new type of inter-organ communication based on circulating bacterial signal molecules, which has broad implications for understanding the mutualistic relationship between microbes and host. | Qin Zhang Ying Pan Benhua Zeng Xiaojiao Zheng Haifang Wang Xueying Shen Hui Li Qian Jiang Jiaxu Zhao Zhuo-Xian Meng Pingping Li Zhengjun Chen Hong Wei Zhihua Liu | 2019 | Cell Research2019，29，7： | 11 |
| 20 | Recombination and Heterologous Expression of Aliophycocyanin Gene in the Chloroplast of Chlamydomonas reinhardtii显示文摘在转基因的工厂的原子核的多基因的异构的表示要求单个基因和随后的回交的介绍重新组成多子单元蛋白质或新陈代谢的小径。以便在一个单个转变事件完成多基因的表示,我们把 allophycocyanin 基因(apcA 和一块印刷电路板) 的大、小的子单元插入了到 Chlamydomonas reinhardtii 叶绿体表示向量,导致 papc-S。构造向量然后被介绍进 C 的叶绿体。由微粒子的轰炸的 reinhardtii。聚合酶链反应和南部的污点分析表明二基因有集成于叶绿体染色体。西方的污点和连接酶的免疫吸着剂试金证明从原核生物的 cyanobacteria 的二基因能正确地在 C 的叶绿体被表示。reinhardtii。在变换蚂蚁的表示外国蛋白质说明了大约全部的可溶性蛋白质的 2%-3% 。这些调查结果在转基因的 C 铺平道路到多子单元蛋白质或新陈代谢的小径的体质。在一个单个转变事件的 reinhardtii 叶绿体。 | Zhong-Liang SU Kai-Xian QIAN Cong-Ping TAN Chun-Xiao MENG Song QIN | 2005 | Acta Biochimica et Biophysica Sinica2005，37，10： | 10 |
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