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| 1 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 2 | Functional genomic studies on an immune-and antiviral-related gene of MyD88 in orange-spotted grouper,Epinephelus coioides显示文摘Myeloid differentiation factor 88(MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB(NF-κB) activation.In this study,a new MyD88 gene(designated as Og-MyD88) was cloned from orange-spotted grouper,Epinephelus coioides,based on the expressed sequence tag(EST) obtained following Roche 454 GS-FLX sequencing.The full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca chuatsi.The deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R(TIR) domain at the carboxyl terminus,as well as three highly conserved motifs(Box1,Box2 and Box3) within the C-terminal TIR domain.In healthy fish,Og-MyD88 was found to be strongly expressed in immune-related tissues,including the spleen,head kidney,kidney,liver,skin and intestine,with lower expression in heart,stomach,brain and muscle.Transcripts of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus(SGIV),a highly lethal viral pathogen to grouper fish.Furthermore,the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-κB activity in HEK-293 cells.Overexpressed Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen(GS) cells.These results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates. | YAN Yang CUI HuaChun WEI JingGuang HUANG YouHua HUANG XiaoHong QIN QiWei | 2012 | Chinese Science Bulletin2012，57，25： | 2 |
| 3 | Optical properties of NAEC-PMMA nonlinear polymeric thin film显示文摘 | Zhihui Qin Changshui Fang Qiwei Pan Qingtian Gu Feng Chen Feng Li Jinzhong Yu | 2002 | Journal of Materials Science2002，，22： | 1 |
| 4 | Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H 2 SO 4 pretreatments in Miscanthus显示文摘 | Fengcheng Li Shuangfeng Ren Wei Zhang Zhengdan Xu Guosheng Xie Yan Chen Yuanyuan Tu Qing Li Shiguang Zhou Yu Li Fen Tu Lin Liu Yanting Wang Jianxiong Jiang Jingping Qin Shizhong Li Qiwei Li Hai-Chun Jing Fasong Zhou Neal Gutterson Liangcai Peng | 2012 | Bioresource Technology2012，，： | 1 |
| 5 | Electron microscopic observations of a marine fish iridovirus isolated from brown-spotted grouper,Epinepheous tauvina显示文摘 | QIN Qiwei LAM T J SIN Y M | 2001 | J Viral Methods2001，98，1： | 1 |
| 6 | Characterization of a novel ranavirus isolated from brown-spotted grouper,Epinephelus tauvina显示文摘 | QIN Qiwei CHANG S F NGOH G H | 2003 | Dis Aquat Organ2003，53，1： | 1 |
| 7 | Functional genomics analysis of Singapore grouper iridovirus:complete sequence determination and proteomic analysis显示文摘 | SONG W J QIN Qiwei QIU J | 2004 | J Virol2004，78，12： | 1 |
| 8 | LAMP for the rapid diagnosis of iridovirus in aquaculture显示文摘Iridoviruses are DNA virus and have caused huge economic losses in the aquaculture industry.The aim of this study was to establish a colorimetric loop-mediated isothermal amplification(LAMP)protocol for the on-site detection of Singapore grouper iridovirus(SGIV).The SGIV-VP61 gene was chosen as the target gene to develop a colorimetric LAMP assay.The optimized condition of the colorimetric LAMP assay was incubation at 63℃ for 1 h.Samples infected with SGIV could be detected with the color change from yellow into pink.The sensitivity of the developed assay is 5.66 copies/μL of the viral DNA template.This sensitivity was about 1000 times higher than that of conventional PCR while it was slightly lower than the one-step semi-nested PCR assay.A total of 60 DNA samples extracted from the fin tissue of the SGIV-infected Asian seabass were examined for SGIV by colorimetric LAMP,semi-nested PCR and conventional PCR.The results of the colorimetric LAMP assay showed 94.87%agreement with the semi-nested PCR.In addition,the DNA extraction method using NaOH showed a better performance in the colorimetric LAMP assay.Taken together,the colorimetric LAMP established was a sensitive,rapid and specific method for the detection of SGIV.SGIV was not detected in samples randomly taken from a genetically improved line of the Asian seabass.However,some seabass obtained from the local markets were found to contain SGIV.Thus,the LAMP assay has the potential application in the diagnosis of iridovirus diseases in the aquaculture industry. | Yepin Yu Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Qiwei Qin Gen Hua Yue | 2022 | Aquaculture and Fisheries2022，7，2： | 0 |
| 9 | Stable perovskite solar cells with 23.12% efficiency and area over 1 cm^(2) by an all-in-one strategy显示文摘To realize the commercialization of perovskite solar cells(PSCs), it is required to overcome the remaining challenges in device enlargement and operational stability. Here, we report an all-in-one strategy by integrating the oxidation of hole-transport material(HTM) with the formation of the passivation layer, which simultaneously solved the stability issues caused by HTM oxidation and realized the uniform defects in passivation over a large area. The resulting devices achieved a certified PCE of23.12% on average with an aperture area of 1.04 cm^(2) and are reproducible with high operational stability because of the exclusion of air exposure, hygroscopic Li-TFSI, and the lithium-based wastes, maintaining ca. 90% of their initial PCEs after operation at the maximum power point under continuous 1 sun illumination for 1,600 h. Our strategy simplifies the fabrication process of PSCs, which is compatible with commercial-scale methods, offering facile access to efficient and stable large-area PSCs. | Hongzhen Su Xuesong Lin Yanbo Wang Xiao Liu Zhenzhen Qin Qiwei Shi Qifeng Han Yiqiang Zhang Liyuan Han | 2022 | Science China Chemistry2022，65，7： | 0 |
| 10 | An optimization of ultra-sonication-assisted extraction from flowers of Apocynum venetum in targeting to amount of free amino acids determined by UPLC-MS/MS显示文摘Amino acids are naturally occurring compounds in many edible or medicinal plants,which possess a variety of nutraceutical effects in human.Here,a method of ultrasound-assisted extraction was optimized using Box-Behnken design of response surface methodology in maximizing the yield of free amino acids deriving from flowers of Apocynum venetum L.Under the optimal condition of ultrasound-assisted extraction,i.e.187.5 W of ultrasonic power,31.93 min of extraction time,and 0.47 mg/ml of solid-liquid ratio,the experimental yield of extractive was 287.17±0.205 mg/g,which was in close agreement with the value,as predicted by the established model.In addition,a hydrophilic interaction ultra-high performance liquid chromatography coupled with QqQ-MS/MS method was developed and validated for simultaneous quantification of 20 types of amino acids without derivatization contained in A.venetum flowers.The analytical method was validated by matrix effect,linearity,limit of detection,limit of quantification,precision,repeatability,stability,and recovery.The analysis results showed that A.venetum flower was rich in free amino acids of~3%of total dried weight,and which contained leucine(13.7 g/mg),isoleucine(7.9 g/mg),and lysine(2.2 g/mg)as the most abundant amino acids.Thus,A.venetum flower could provide beneficial nutrient values for human health,e.g.adult weight maintenance or glucose homeostasis. | Yan Jin Caroline Yang Wang Weihui Hu Yun Huang Miranda Li Xu Huaiyou Wang Xiangpeng Kong Yicun Chen Tina Tingxia Dong Qiwei Qin Karl Wah Keung Tsim | 2019 | Food Quality and Safety2019，3，1： | 0 |
| 11 | Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea显示文摘DNA methylation has been proposed to regulate plant stress resistance.However,the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood.Here,we present single-base maps of the DNA methylomes of mulberry(Morus notabilis)leaves that were subjected to a mock treatment or inoculation with Botrytis cinerea.Compared with the former,the latter showed decreased mCG and mCHG levels and increased mCHH levels.DNA methylation inhibitors reduced resistance gene methylation levels and enhanced mulberry resistance,suggesting that the hypomethylation of resistance genes affects mulberry resistance to B.cinerea.Virus-induced gene silencing of MnMET1 enhanced the expression of mulberry-resistance genes,thereby increasing the plant’s resistance to B.cinerea.We also found that MITEs play a dominant role in controlling DNA methylation levels.MITEs appear to be the main sources of 24-nt siRNAs that regulate gene expression through the RNA-directed DNA methylation pathway. | Youchao Xin Bi Ma Qiwei Zeng Wenmin He Meiling Qin Ningjia He | 2021 | Horticulture Research2021，8，1： | 0 |
| 12 | Slc43a2^(+)T cell metastasis from spleen to brain in RGNNV infected teleost显示文摘The origin of T cells in the teleost's brain is unclear.While viewing the central nervous system(CNS)as immune privileged has been widely accepted,previous studies suggest that T cells residing in the thymus but not in the spleen of the teleost play an essential role in communicating with the peripheral organs.Here,we identified nine T cell subpopulations in the thymus and spleen of orange-spotted grouper(Epinephelus coioices)through single-cell RNA-sequencing analysis.After viral CNS infection with red-spotted grouper nervous necrosis virus(RGNNV),the number of slc43a2^(+)T cells synchronously increased in the spleen and brain.During the infection tests in asplenic zebrafish(tlx1^▲zebrafish model),no increase in the number of slc43a2^(+)T cells was observed in the brain.Single-cell transcriptomic analysis indicated that slc43a2^(+)T cells mature and functionally differentiate within the spleen and then migrate into the brain to trigger an immune response.This study suggests a novel route for T cell migration from the spleen to the brain during viral infection in fish. | Qing Wang Yali Liu Minlin Zhang Min Yang Jiantao Liang Xiaoling Zuo Shaowen Wang Xianze Jia Huihong Zhao Han Jiang Qiang Lin Qiwei Qin | 2024 | Science China(Life Sciences)2024，67，4： | 0 |
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