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| 1 | The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)显示文摘Hybridization can combine the genomes of different strains or species, which leads to changes of genotype and phenotype in the hybrids. In this study, we aimed to investigate the genetic variations of hybrids(WR-F1 and WR-F2) derived from the intraspecific hybridization of white crucian carp(Carassius auratus cuvieri, WCC, ♀) and red crucian carp(Carassius auratus red var., RCC, ♂). Here, we compared the orthologous genes in the liver transcriptomes of hybrids with those of WCC and RCC, and classified the orthologous genes into eight gene patterns within three categories(chimera, mutant, and biparental origin genes).The results revealed 19.04%, 4.17% chimeric genes and 6.90%, 5.05% mutations of orthologous genes in WR-F1 and WR-F2 respectively. Seventeen of twenty-three characterized genes(77%) were confirmed to be the chimeras at the genomic DNA level.The GO classification discovered that some chimeric and mutant genes were related to metabolic process, immune system and developmental process in WR-F1. Our results provide the new evidence that hybridization can combine the parental genomes,leading to changes in the genotype of the resultant hybrids. This is the first report on the formation of chimeric genes from fish intraspecific hybridization, which is potentially interesting from the context of both evolution and the genetic breeding of fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Xiujuan Xu Fangzhou Hu Jing Wang Jun Xiao Shi Wang Wuhui Li Min Tao Qinbo Qin Rurong Zhao Zhanzhou Yao Shaojun Liu | 2018 | Science China(Life Sciences)2018，61，9： | 6 |
| 2 | Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny显示文摘The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with heterosis.Gene knockout technique provides a safe and acceptant way for fish breeding.Achieving gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization breeding.Tyrosinase(TYR) is a key enzyme in melanin synthesis.Depletion of tyr in zebrafish and mice results in mosaic pigmentation or total albinism.Here,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant WCC.Both the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,i.e.tyrp1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin synthesis.These results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical fish.Furthermore,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min Tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu | 2019 | Science China(Life Sciences)2019，62，9： | 4 |
| 3 | Effect of casting speed on surface quality of horizontal direct chill casting 7075 aluminum alloy ingot显示文摘7075 aluminum alloy ingot with the diameter of 100 mm has been produced by hor-izontal direct chill casting in different casting speed. The effect of the casting speed on the ingot surface and subsurface layer was studied by surface observation and subsurface structure analysis. It was found that increasing the casting speed results in the adding of segregation knots in the ingot surface. The thickness of the dendrite microstructure layer in the subsurface reduces with increasing the casting speed. And the elements of Zn,Cu and Mg enrich in the coarse dendrite microstructure layer of the ingot. | Qingfeng ZHU Zhihao ZHAO Jianzhong CUI Xiangjie WANG Ke QIN | 2011 | Acta Metallurgica Sinica(English Letters)2011，24，5： | 3 |
| 4 | Hot-top direct chill casting assisted by a twin-cooling field:Improving the ingot quality of a large-size 2024 Al alloy显示文摘Microstructure inhomogeneity and negative segregation have long been challenges for large-size alloy ingots,directly affecting the downstream processing and final performance of products.Here,we used2024 aluminum alloy as a model alloy to propose a technique,named double-cooling field casting,i.e.,one 2024 Al alloy rod(Φ20 mm)at room temperature was introduced into the melt along the central axis of the hot-top with the protection of a thermal-insulation tube during the direct chill(DC)casting process of aΦ300 mm 2024 Al alloy ingot.The results show that the introduction of the same alloy solid insert has a remarkable influence on refining grains in the center region of the ingot,reducing negative centerline segregation and decreasing the depth of the center part of the sump.With the application of the 2024 Al insert,the mean size of equiaxed grains at the center part of the ingot decreased from1204±132μm to 721±69μm.The relative deviation of the Cu and Mg main solutes reduced from-0.062 and-0.054 to-0.03 and-0.024,respectively,and the sump depth decreased from 280 mm to242 mm.Moreover,the shape of the solidification front was changed from‘V’-shaped to‘W’-shaped.The ingot quality was thus improved,mainly arising from the dissolution of the cold 2024 Al insert at a proper position of the hot-top counteracting some latent heat of solidification of the ingot,dissipating the heat of the central part of the hot-top by conducting the 2024 Al insert to the outside,and providing extra-nuclei from the unmoltenα-Al particles of the insert. | Cheng Zhu Zhihao Zhao Qingfeng Zhu Gaosong Wang Yubo Zuo Qiangqiang Li Gaowu Qin | 2022 | Journal of Materials Science & Technology2022，，17： | 1 |
| 5 | A joint call for actions to advance taxonomy in China显示文摘Taxonomy plays an important role in understanding the origin, evolution, and ecological functionality of biodiversity. There are large number of unknown species yet to be described by taxonomists, which together with their ecosystem services cannot be effectively protected prior to description. Despite this, taxonomy has been increasingly underrated insufficient funds and permanent positions to retain young talents. Further, the impact factordriven evaluation systems in China exacerbate this downward trend, so alternative evaluation metrics are urgently necessary. When the current generation of outstanding taxonomists retires,there will be too few remaining taxonomists left to train the next generation. In light of these challenges, all co-authors worked together on this paper to analyze the current situation of taxonomy and put out a joint call for immediate actions to advance taxonomy in China. | Chaodong Zhu Arong Luo Ming Bai Michael COrr Zhonge Hou Siqin Ge Jun Chen Yibo Hu Xuming Zhou Gexia Qiao Hongzhi Kong Limin Lu Xiaohua Jin Lei Cai Xinli Wei Ruilin Zhao Wei Miao Qingfeng Wang Zhongli Sha Qiang Lin Meng Qu Jianping Jiang Jiatang Li Jing Che Xuelong Jiang Xiaoyong Chen Lianming Gao Zongxin Ren Chunlei Xiang Shixiao Luo Donghui Wu Dong Liu Yanqiong Peng Tao Su Chenyang Cai Tianqi Zhu Wanzhi Cai Xingyue Liu Hu Li Huaijun Xue Zhen Ye Xuexin Chen Pu Tang Shujun Wei Hong Pang Qiang Xie Feng Zhang Feng Zhang Xianjin Peng Aibing Zhang Taiping Gao Changfa Zhou Chen Shao Libin Ma Zhaoming Wei Yunxia Luan Ziwei Yin Wu Dai Cong Wei Xiaolei Huang Jingxian Liu Xiangsheng Chen Tianci Yi Zhisheng Zhang Zhulidezi Aishan Qin Li Hongying Hu | 2022 | Zoological Systematics2022，47，3： | 1 |
| 6 | Surface modification of steels and magnesium alloy by high current pulsed electron beam显示文摘 | Shengzhi Hao Bo Gao Aimin Wu Jianxin Zou Ying Qin Chuang Dong Jian An Qingfeng Guan | 2005 | Nuclear Inst and Methods in Physics Research B2005，，3： | 1 |
| 7 | Temperature–stress fields and related phenomena induced by a high current pulsed electron beam显示文摘 | Ying Qin Jianxin Zou Chuang Dong Xiaogang Wang Aimin Wu Yue Liu Shengzhi Hao Qingfeng Guan | 2004 | Nuclear Inst and Methods in Physics Research B2004，，4： | 1 |
| 8 | Surface modification of steels and magnesium alloy by high current pulsed electron beam显示文摘 | Hao Shengzhi Gao Bo Wu Aimin Zou Jianxin Qin Yin Dong Chuang An Jian Guan Qingfeng | 2005 | Nuclear Instruments and Methods in Physics Research B2005，240，： | 1 |
| 9 | Geochemistry of rare earth elements and yttrium in Late Permian coals from the Zhongliangshan coalfield, southwestern China显示文摘Rare earth elements and yttrium(REY)in coal deposits are considered promising alternative sources for these resources owing to their increasing global demand.This paper reports the geochemical characteristics of REY in the Late Permian coals from an underground K1a seam section of the Zhongliangshan coalfield in Chongqing,southwestern China.The mineralogy,degree of enrichment,distribution patterns,modes of occurrence,and sediment origin of REY were investigated.Compared with the average of world coals,the concentration of REY in the K1a coals were normal,dominated by light REY(LREY),with less medium and heavy REY(MREY,HREY).The fractionation degree of the MREY and HREY are higher than that of LREY in most K1a coal samples,deduced from the mixed enrichment type of REY,mainly including M-H-type,and a few L-M type and H-type.In addition,the combination of anomalies of Ce,Eu,Gd,and Al_(2)O_(3)/TiO_(2) parameters,the terrigenous materials in the K1a coal were derived from the felsic-intermediate rocks at the top of the Emeishan basalt sequence,and the samples were affected by seawater intrusion during early peat accumulation.Although the minerals primarily consist of kaolinite,illite,pyrite,and small amounts of quartz,calcite and anatase,REY are correlated with ash yield,SiO2,and Al_(2)O_(3),revealing that the REY mainly occur in aluminosilicate minerals,especially kaolinite and illite.Meanwhile,REY positively relate to P_(2)O_(5) and Zr,which may exist in phosphate-containing minerals or zircon.Furthermore,most samples in the K1a coal or ash do not reach the cut-off grade for the beneficial recovery of REY.With the exception of central Guizhou,southwestern Chongqing,and the junction of western Guizhou and northeastern Yunnan,the REY content in coals from southwestern China are high,and its by-products are suitable as potential REY sources. | Qingfeng LU Shenjun QIN Hongyang BAI Wenfeng WANG De’e QI Xin HE Bofei ZHANG | 2023 | Frontiers of Earth Science2023，17，1： | 0 |
| 10 | Elementary discussion on construction technology of foundation treatment for water conservancy and hydropower projects显示文摘Human life is inseparable from water resources,and human development is inseparable from electric energy.Water conservancy and hydropower project plays a very important role in all stages of human development,whether it is power generation,irrigation,flood control or drainage,which can not be separated from the construction of water and hydropower projects.This paper briefly discusses and analyzes the basic treatment construction techniques for water conservancy and hydropower projects,giving full play to the key role of water conservancy and hydropower projects in the development of national economy. | Linjun Shao Qingfeng Wang Guiyun Qin Rongqiang Liu | 2018 | Smart Construction Research2018，2，1： | 0 |
| 11 | A novel protein complex that regulates active DNA demethylation in Arabidopsis显示文摘Active DNA demethylation is critical for altering DNA methylation patterns and regulating gene expression.The 5-methylcytosine DNA glycosylase/lyase ROS1 initiates a base-excision repair pathway for active DNA demethylation and is required for the prevention of DNA hypermethylation at 1000 s of genomic regions in Arabidopsis.How ROS1 is regulated and targeted to specific genomic regions is not well understood.Here,we report the discovery of an Arabidopsis protein complex that contains ROS1,regulates ROS1 gene expression,and likely targets the ROS1 protein to specific genomic regions.ROS1 physically interacts with a WD40 domain protein(RWD40),which in turn interacts with a methyl-DNA binding protein(RMB1)as well as with a zinc finger and homeobox domain protein(RHD1).RMB1 binds to DNA that is methylated in any sequence context,and this binding is necessary for its function in vivo.Loss-of-function mutations in RWD40,RMB1,or RHD1 cause DNA hypermethylation at several tested genomic regions independently of the known ROS1 regulator IDM1.Because the hypermethylated genomic regions include the DNA methylation monitoring sequence in the ROS1 promoter,plants mutated in RWD40,RMB1,or RHD1 show increased ROS1 expression.Importantly,ROS1 binding to the ROS1 promoter requires RWD40,RMB1,and RHD1,suggesting that this complex dictates ROS1 targeting to this locus.Our results demonstrate that ROS1 forms a protein complex with RWD40,RMB1,and RHD1,and that this novel complex regulates active DNA demethylation at several endogenous loci in Arabidopsis. | Pan Liu Wen-Feng Nie Xiansong Xiong Yuhua Wang Yuwei Jiang Pei Huang Xueqiang Lin Guochen Qin Huan Huang Qingfeng Niu Jiamu Du Zhaobo Lang Rosa Lozano-Duran Jian-Kang Zhu | 2021 | Journal of Integrative Plant Biology2021，63，4： | 0 |
| 12 | Elementary discussion on construction technology of foundation treatment for water conservancy and hydropower projects显示文摘Human life is inseparable from water resources,and human development is inseparable from electric energy.Water conservancy and hydropower project plays a very important role in all stages of human development,whether it is power generation,irrigation,flood control or drainage,which can not be separated from the construction of water and hydropower projects.This paper briefly discusses and analyzes the basic treatment construction techniques for water conservancy and hydropower projects,giving full play to the key role of water conservancy and hydropower projects in the development of national economy. | Linjun Shao Qingfeng Wang Guiyun Qin Rongqiang Liu | 2018 | Smart Construction Research2018，2，3： | 0 |
| 13 | Comparative Analysis of Forest Conservation and Sustainable Forest Management in China and Germany显示文摘Forestry development in Germany and China does not seem to be comparable due to differences in nature,politics,economy,society,culture and demographic situation,but the comparative study on these still has great important theoretical and practical significance.Taking into account the significant differences between the two countries,a comparative analysis was carried out through following aspects from(1)forestry policies,strategies and programs,(2)legal regulatory,administrative and socio-economic framework conditions,(3)tools and provisions and other aspects of bilateral forest protection and sustainable forest management.Researches on forest protection and sustainable forest management framework were carried out by combination of forestry development and historical review discussion in order to learn from the German experiences to promote the development of China's forestry. | Ying ZHANG Qingfeng QIN Jieyu FAN Ke CHEN | 2013 | Asian Agricultural Research2013，5，10： | 0 |
| 14 | Design and Research of 324MHz Waveguide Window显示文摘Waveguide windows play a role in isolating the ultra-high vacuum from the atmosphere or the inflated state in high-power microwave systems and high-power irradiation accelerators.In addition,in the process of transmitting RF electromagnetic waves,as much power as possible,needs to be fed into the cavity,so insertion loss and reflection loss need to be controlled in the smallest range as possible.When designing the waveguide window,it is mainly to design the structure of the window and select appropriate ceramic sheet parameters to reduce the probability of the waveguide window being damaged by ignition,so that the waveguide window can transmit enough high power and ensure that the accelerator can be in a long-term stable working state,so as to improve the performance of the waveguide window. | QIN Cheng WANG Xiulong ZHU Zhibin WU Qingfeng YANG Yu | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
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