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| 1 | Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs显示文摘 | Qiwei Shan Yanpeng Wang Kunling Chen Zhen Liang Jun Li Yi Zhang Kang Zhang Jinxing Liu Daniel F. Voytas Xuelian Zheng Yong Zhang Caixia Gao | 2013 | Molecular Plant2013，6，4： | 37 |
| 2 | Desmoglein 2(DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia显示文摘Human adenovirus type 55(HAdV-B55) is a re-emergent acute respiratory disease pathogen that causes adult communityacquired pneumonia(CAP). Previous studies have shown that the receptor of HAdV-B14, which genome is highly similar with HAdV-B55, is human Desmoglein 2(DSG2). However, whether the receptor of HAdV-B55 is DSG2 is undetermined because there are three amino acid mutations in the fiber gene between HAdV-B14 and HAdV-B55. Here, firstly we found the 3T3 cells, a mouse embryo fibroblast rodent cell line which does not express human DSG2, were able to be infected by HAdV-B55 after transfected with pcDNA3.1-DSG2, while normal 3T3 cells were still unsusceptible to HAdV-B55 infection. Next, A549 cells with h DSG2 knock-down by siRNA were hard to be infected by HAdV-B3/-B14/-B55, while the control siRNA group was still able to be infected by all these types of HAdVs. Finally, immunofluorescence confocal microscopy indicated visually that Cy3-conjugated HAdV-B55 viruses entered A549 cells by binding to DSG2 protein.Therefore, DSG2 is a major receptor of HAdV-B55 causing adult CAP. Our finding is important for better understanding of interactions between adenoviruses and host cells and may shed light on the development of new drugs that can interfere with these processes as well as for the development of potent prophylactic vaccines. | Jing Zhang Kui Ma Xiangyu Wang Yinbo Jiang Shan Zhao Junxian Ou Wendong Lan Wenyi Guan Xiaowei Wu Heping Zheng Bin Yang Chengsong Wan Wei Zhao Jianguo Wu Qiwei Zhang | 2021 | Virologica Sinica2021，36，6： | 4 |
| 3 | A gene that underwent adaptive evolution, LAC2 (LACCASE), in Populus euphratica improves drought tolerance by improving water transport capacity显示文摘Drought severely limits plant development and growth;accordingly,plants have evolved strategies to prevent water loss and adapt to water deficit conditions.However,experimental cases that corroborate these evolutionary processes are limited.The LACCASEs(LACs)family is involved in various plant development and growth processes.Here,we performed an evolutionary analysis of LACs from Populus euphratica and characterized the functions of LACs in Arabidopsis and poplar.The results showed that in PeuLACs,multiple gene duplications led to apparent functional redundancy as the result of various selective pressures.Among them,PeuLAC2 underwent strong positive selection.Heterologous expression analyses showed that the overexpression of PeuLAC2 alters the xylem structure of plants,including thickening the secondary cell wall(SCW)and increasing the fiber cell length and stem tensile strength.Altogether,these changes improve the water transport capacity of plants.The analysis of the physiological experimental results showed that PeuLAC2-OE lines exhibited a stronger antioxidant response and greater drought tolerance than WT.Three genes screened by transcriptome analysis,NAC025,BG1,and UGT,that are associated with SCW synthesis and drought stress were all upregulated in the PeuLAC2-OE lines,implying that the overexpression of PeuLAC2 thickened the SCW,improved the water transport capacity of the plant,and further enhanced its drought tolerance.Our study highlights that genes that have undergone adaptive evolution may participate in the development of adaptive traits in P.euphratica and that PeuLAC2 could be a candidate gene for molecular genetic breeding in trees. | Zhimin Niu Guiting Li Hongyin Hu Jiaojiao Lv Qiwei Zheng Jianquan Liu Dongshi Wan | 2021 | Horticulture Research2021，8，1： | 2 |
| 4 | The C_(6)D_(6)detector system on the Back-n beam line of CSNS显示文摘Introduction The neutron capture cross sections are very important in the field of nuclear device design and basic physics research.Hydrogen-free liquid scintillator such as C_(6)D_(6)detectors are widely used in the neutron capture cross-sectional measurements for the low neutron sensitivity and fast time response.The Back-n white neutron source at China Spallation Neutron Source is the first spallation white neutron source in China,and it is suitable for neutron capture cross-sectional measurement.Materials and methods A C_(6)D_(6)detector system was built in the Back-n experimental station.The pulse height weighting technique was used to determine the system’s detection efficiency.The response to gamma rays of the C_(6)D_(6)detector was measured,and the energy resolution function was determined.Monte Carlo simulation with Geant4 code was carried out to get the weighting function of this C_(6)D_(6)detector system.Additionally,the systematic uncertainty of the weighting function was also determined.Conclusion According to the experimental and simulation results,this C_(6)D_(6)detector system can be used to measure neutron capture cross section. | Jie Ren Xichao Ruan Jie Bao Guangyuan Luan Wei Jiang Qi An Huaiyong Bai Ping Cao Qiping Chen Yonghao Chen Pinjing Cheng Zengqi Cui Ruirui Fan Changqing Feng Minhao Gu Fengqin Guo Changcai Han Zijie Han Guozhu He Yongcheng He Yuefeng He Hanxiong Huang Weiling Huang Xiru Huang Xiaolu Ji Xuyang Ji Haoyu Jiang Hantao Jing Ling Kang Mingtao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Rong Liu Shubin Liu Xingyan Liu Yinglin Ma Changjun Ning Binbin Qi Zhaohui Song Hong Sun Xiaoyang Sun Zhijia Sun Zhixin Tan Hongqing Tang Jingyu Tang Pengcheng Wang Qi Wang Taofeng Wang Yanfeng Wang Zhaohui Wang Zheng Wang Jie Wen Zhongwei Wen Qingbiao Wu Xiaoguang Wu Xuan Wu Likun Xie Yiwei Yang Han Yi Li Yu Tao Yu Yongji Yu Guohui Zhang Jing Zhang Linhao Zhang Liying Zhang Qingmin Zhang Qiwei Zhang Xianpeng Zhang Yuliang Zhang Zhiyong Zhang Yingtan Zhao Liang Zhou Zuying Zhou Danyang Zhu Kejun Zhu Peng Zhu | 2019 | Radiation Detection Technology and Methods2019，3，3： | 1 |
| 5 | Identification and characterization of a novel subgenotype of bovine viral diarrhea virus isolated from dairy cattle in Northwestern China显示文摘 | Xiaowei Gong Xiaoan Cao Fuying Zheng Qiwei Chen Jizhang Zhou Hong Yin Lihong Liu Xuepeng Cai | 2013 | Virus Genes2013，，2： | 1 |
| 6 | Investigating the function of Akt by tet-off inducible expression system显示文摘A tet-off inducible cell line named BBT derived from BA/F3β cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BBT cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis. | Qiwei Zhai Hongbin Ji Zhongcheng Zheng Lanying Sun Xinyuan Liu | 2001 | Chinese Science Bulletin2001，46，3： | 1 |
| 7 | Online monitoring of volatile organic compound production and emission during sewage sludge composting显示文摘 | Yujun Shen Tong-Bin Chen Ding Gao Guodi Zheng Hongtao Liu Qiwei Yang | 2012 | Bioresource Technology2012，，： | 1 |
| 8 | Nmnat2 delays axon degeneration in superior cervical ganglia dependent on its NAD synthesis activity显示文摘 | Tingting Yan Yan Feng Jin Zheng Xinjian Ge Yi Zhang Dongmei Wu Jian Zhao Qiwei Zhai | 2009 | Neurochemistry International2009，，1： | 1 |
| 9 | AHA1 upregulates IDH1 and metabolic activity to promote growth and metastasis and predicts prognosis in osteosarcoma显示文摘Osteosarcoma(OS)is the most common primary malignant bone tumor in children and adolescents.Although activator of HSP90 ATPase activity 1(AHA1)is reported to be a potential oncogene,its role in osteosarcoma progression remains largely unclear.Since metabolism reprogramming is involved in tumorigenesis and cancer metastasis,the relationship between AHA1 and cancer metabolism is unknown.In this study,we found that AHA1 is significantly overexpressed in osteosarcoma and related to the prognosis of osteosarcoma patients.AHA1 promotes the growth and metastasis of osteosarcoma both in vitro and in vivo.Mechanistically,AHA1 upregulates the metabolic activity to meet cellular bioenergetic needs in osteosarcoma.Notably,we identifed that isocitrate dehydrogenase 1(IDH1)is a novel client protein of Hsp90 AHA1.Furthermore,the IDH1 protein level was positively correlated with AHA1 in osteosarcoma.And IDH1 overexpression could partially reverse the effect of AHA1 knockdown on cell growth and migration of osteosarcoma.Moreover,high IDH1 level was also associated with poor prognosis of osteosarcoma patients.This study demonstrates that AHA1 positively regulates IDH1 and metabolic activity to promote osteosarcoma growth and metastasis,which provides novel prognostic biomarkers and promising therapeutic targets for osteosarcoma patients. | Diwei Zheng Weihai Liu Wenlin Xie Guanyu Huang Qiwei Jiang Yang Yang Jiarong Huang Zihao Xing Mengling Yuan Mengning Wei Yao Li Junqiang Yin Jingnan Shen Zhi Shi | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 1 |
| 10 | Interleukin-2 mediates the peripheral analgesia of interferon alpha显示文摘Interferon-α (IFN-α) and interleukin-2 (IL-2) are crucial cytokines in immune system. They also play an important role in nerve system. It has been reported that IL-2 can induce the central analgesia. It is demonstrated that IFN-α also can induce the peripheral analgesia, which can be blocked by naloxone as IL-2. Furthermore, the analgesic effect of IFN-α is reversible by monoclonal anti-IL-2 antibody. In vitro experiments show that IFN-α significantly increases the production of IL-2 in a dose dependent manner. These data suggest that IL-2 mediates the peripheral analgesia of IFN-α. | Li Li Qiwei Zhai Wei Tang Tong Wu Zhongcheng Zheng Xinyuan Liu | 1999 | Chinese Science Bulletin1999，44，3： | 0 |
| 11 | Integrated global analysis in spider flowers illuminates features underlying the evolution and maintenance of C_(4) photosynthesis显示文摘The carbon concentrating mechanism—C_(4) photosynthesis—represents a classic example of convergent evolution,but how this important trait originated and evolved remains largely enigmatic.The spider flower Gynandropsis gynandra is a valuable leafy vegetable crop and medicinal plant that has also been recognized as a C_(4) model species.Here we present a high-quality chromosome-scale annotated genome assembly of G.gynandra through a combination of Oxford Nanopore Technology(ONT),HiFi and Hi-C technology.The 17 super-scaffolds cover 98.66% of the estimated genome(997.61 Mb),with a contig N50 of 11.43 Mb and a scaffold N50 of 51.02 Mb.Repetitive elements occupy up to 71.91% of its genome,and over half are long terminal repeat retrotransposons(LTR-RTs)derived from recent bursts,contributing to genome size expansion.Strikingly,LTR-RT explosion also played a critical role in C_(4) evolution by altering expression features of photosynthesis-associated genes via preferential insertion in promoters.Integrated multiomics analyses of G.gynandra and the ornamental horticulture C3 relative Tarenaya hassleriana reveal that species-specific whole-genome duplication,gene family expansion,recent LTR–RT amplification,and more recent tandem duplication events have all facilitated the evolution of C_(4) photosynthesis,revealing uniqueness of C_(4) evolution in the Cleome genus.Moreover,high leaf vein density and heat stress resilience are associated with shifted gene expression patterns.The mode of C_(3)-to-C_(4) transition found here yields new insights into evolutionary convergence of a complex plant trait.The availability of this reference-grade genomic resource makes G.gynandra an ideal model system facilitating efforts toward C4-aimed crop engineering. | Wei Zhao Jun Li Xingchao Sun Qiwei Zheng Jing Liu Wei Hua Jun Liu | 2023 | Horticulture Research2023，10，8： | 0 |
| 12 | Rhodium-Catalyzed Asymmetric Transfer Hydrogenation of Heterocyclic Diaryl Ketones:Facile Access to Key Intermediate of Baloxavir显示文摘Transition metal-catalyzed asymmetric transfer hydrogenation has been proven to be a powerful approach for the synthesis of chiral alcohols.Herein,a highly efficient and enantioselective transfer hydrogenation of dibenzoheptaheterocyclic ketones catalyzed by an arene-tethered TsDPEN-based Rh(ll)catalyst has been successfully developed,and a variety of dibenzoheptaheterocyclic ketones were reduced by a 1/1 mixture of formic acid and DBU(1,8-diazabicyclo[5.4.0]undec-7-ene)with high yields and enantioselectivities.With this method,the asymmetric reduction of 7,8-difluorodibenzo[b,e]thiepin-11(6H)-one has been realized,providing the key intermediate of baloxavir marboxil with>99% yield and>99% ee at a substrate/catalyst molar ratio of 1000. | Li Wang Renwei Xiao Jingyuan Song Long-Sheng Zheng Qiwei Lang Gen-Qiang Chen Xumu Zhang | 2024 | Chinese Journal of Chemistry2024，42，1： | 0 |
| 13 | Co-expression of Clostridium perfringens α and ε Toxins and Their Immunogenicity显示文摘The gene CPA and ETX encoding α and ε toxins were amplified from the standard strain C60-2 of type D Clostridium perfingens by PCR,and then they were inserted into pet-Duet-1 vecto,respectively for the construction of co-expression plasmid p ETDuet-1-CPA-ETX. The co-expression plasmid was transformed into BL21( DE3) competent cells. α and ε toxins proteins were induced by IPTG before analysis by SDS-PAGE. As a result,both of α and ε toxins were detected at 43 and 34 KU by western-blot. Mice immunized with the co-expressed α and ε toxins proteins produced high titers of neutralizing antibodies in the serum,which protected the mice against the attack of type D C. perfringens culture filtrate. In addition,mice immunized with the produced co-expressed α and ε toxins proteins showed significantly higher surviving rate than with ε toxins protein alone when infected with culture filtrate of type D C. perfringens. These results indicated that co-expression of α and ε toxins proteins could be used as a new method to prepare vaccines against the pathogens of multiple serotypes. | Han Guangwei Li Zhaocai Gong Xiaowei Chen Qiwei Cao Xiaoan Zheng Fuying Zhang Xiaoying Zhou Jizhang | 2015 | Animal Husbandry and Feed Science2015，7，2： | 0 |
| 14 | Asyncflow: A visual programming tool for game artificial intelligence显示文摘Visual programming tools are widely applied in the game industry to assist game designers in developing game artificial intelligence(game AI)and gameplay.However,testing multiple game engines is a time-consuming operation,which degrades development efficiency.To provide an asynchronous platform for game designers,this paper introduces Asyncflow,an open-source visual programming solution.It consists of a flowchart maker for game logic explanation and a runtime framework integrating an asynchronous mechanism based on an event-driven architecture.Asyncflow supports multiple programming languages and can be easily embedded in various game engines to run flowcharts created by game designers.©2021 The Authors. | Zhipeng Hu Changjie Fan Qiwei Zheng Wei Wu Bai Liu | 2021 | Visual Informatics2021，5，4： | 0 |
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