|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization显示文摘Te efcient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine,while the dilemma of metal catalysis,uneasy premodifcation,and limited reaction efciency in traditional bioconjugation has restricted the booming development to some extent.Here,we provide a strategy for metal-free click bioconjugation at diverse levels based on activated alkynes.As a proof-of-concept,the abundant native groups including amine,thiol,and hydroxyl groups can directly react with activated alkynes without any modifcation in the absence of metal catalysis.Trough this strategy,high-efcient modifcation and potential functionalization can be achieved for natural polysaccharide,biocompatible polyethylene glycol(PEG),synthetic polymers,cell penetrating peptide,protein,fast whole-cell mapping,and even quick diferentiation and staining of Gram-positive bacteria,etc.Terefore,current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fuorescence labeling and functional modifcation of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection. | Xianglong Hu Xueqian Zhao Benzhao He Zheng Zhao Zheng Zheng Pengfei Zhang Xiujuan Shi Ryan T.K.Kwok Jacky W.Y.Lam Anjun Qin Ben Zhong Tang | 2018 | Research2018，，1： | 7 |
| 2 | The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)显示文摘Hybridization can combine the genomes of different strains or species, which leads to changes of genotype and phenotype in the hybrids. In this study, we aimed to investigate the genetic variations of hybrids(WR-F1 and WR-F2) derived from the intraspecific hybridization of white crucian carp(Carassius auratus cuvieri, WCC, ♀) and red crucian carp(Carassius auratus red var., RCC, ♂). Here, we compared the orthologous genes in the liver transcriptomes of hybrids with those of WCC and RCC, and classified the orthologous genes into eight gene patterns within three categories(chimera, mutant, and biparental origin genes).The results revealed 19.04%, 4.17% chimeric genes and 6.90%, 5.05% mutations of orthologous genes in WR-F1 and WR-F2 respectively. Seventeen of twenty-three characterized genes(77%) were confirmed to be the chimeras at the genomic DNA level.The GO classification discovered that some chimeric and mutant genes were related to metabolic process, immune system and developmental process in WR-F1. Our results provide the new evidence that hybridization can combine the parental genomes,leading to changes in the genotype of the resultant hybrids. This is the first report on the formation of chimeric genes from fish intraspecific hybridization, which is potentially interesting from the context of both evolution and the genetic breeding of fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Xiujuan Xu Fangzhou Hu Jing Wang Jun Xiao Shi Wang Wuhui Li Min Tao Qinbo Qin Rurong Zhao Zhanzhou Yao Shaojun Liu | 2018 | Science China(Life Sciences)2018，61，9： | 6 |
| 3 | New ancient DNA sequences suggest high genetic diversity for the woolly mammoth (Mammuthus primigenius)显示文摘细胞色素 b 基因(mtDNA ) 的部分 DNA 序列成功地从从 Xiguitu 县(Yakeshi ) 收集的 Mammuthus primigenius 的迟了的更新世石块骨头被检索,内部蒙古自治区域并且从 Zhaodong,在北中国的 Heilongjiang 省的哈尔滨。二古老的 DNA 碎片(109 bp 和 124 bp ) 被可再现的实验与另外的 Elephantidae 税在二个不同实验室并且由种系发生的分析证实一。用在分开或联合的数据集的这些序列和出版数据的种系发生的分析在毛茸茸的猛犸和二头生活大象之中显示不稳定的关系, Elephas 和 Laxodonta。除了过去常尝试 Elephantidae 终端的长独立的进化的短序列征税一,我们建议高 intra 特定的差异在 Mammuthus primigeniuscrossing 存在空间、时间的范围,为 DNA 导致建筑群和分叉的基因背景到目前为止定序恢复。在绝灭的毛茸茸的猛犸的高基因的差异能解释 Elephantidae 税的明显的不稳定性一上分子的种系发生的树和罐头关于未解决的 Elephantidae 三分法调解明显的悖论。 | YANG Shujuan LAI Xulong SHI Suhua CHENG Shenghong ZHOU Xiugao YANG Xiujuan YANG Hong | 2006 | Progress in Natural Science:Materials International2006，16，4： | 5 |
| 4 | A novel receptor-binding domain (RBD)-based mRNA vaccine against SARS-CoV-2显示文摘Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)highlights the need to develop effective and safe vaccines.Similar to SARS-CoV,SARS-CoV-2 recognizes angiotensin-converting enzyme 2(ACE2)as receptor for host cell entry.1,2 SARS-CoV-2 spike(S)protein consists of S1,including receptor-binding domain(RBD),and S2 subunits.3,4 We previously demonstrated that RBDs of SARS-CoV and MERS-CoV serve as important targets for the development of effective vaccines. | Wanbo Tai Xiujuan Zhang Aleksandra Drelich Juan Shi Jason CHsu Larry Luchsinger Christopher D.Hillyer Chien-Te KTseng Shibo Jiang Lanying Du | 2020 | Cell Research2020，30，10： | 5 |
| 5 | Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin显示文摘The interactions between bovine serum albumin(BSA) and gold nanoparticles(AuNPs) ,and the conformational changes of BSA induced by this interaction,were investigated by UV-visible absorption spectroscopy,fluorescence spectroscopy,and Fourier transform infrared in combination with attenuated total reflection spectroscopy(ATR-FTIR) .The critical adsorption density for preventing AuNP aggregation in 0.1 mol/L phosphate buffered saline(pH 7.2) was 23 BSA molecules per gold particle or 3.8×1012 BSA molecules/cm2.BSA bound to the AuNPs with high affinity(binding constant Ks=7.59×108 L/mol) ,and the intrin-sic fluorescence of BSA was quenched by the AuNPs in accordance with the static quenching mechanism.Both fluorescence spectroscopy and ATR-FTIR showed that AuNPs induced conformational changes in BSA,which resulted in it becoming less compact and increased the polarity of the microenvironment around the tryptophan residue Trp-212. | SHI XiuJuan LI Dan XIE Jing WANG Shawn WU ZhaoQiang CHEN Hong | 2012 | Chinese Science Bulletin2012，57，10： | 2 |
| 6 | A novel purification technique for noble gas isotope analyses of authigenic minerals显示文摘高贵、活跃的气体为高贵煤气的同位素分析在煤气的抽取期间被免除地质的样品。活跃气体应该为分析在 inletting 前被移开到集体分光计。准备系统能在很地质的样品上面清洗的正常高贵气体。然而,从油 / 气体地里的沉积岩石的 authigenic 矿物质包含有机物,它不能被正常准备系统清理并且因此影响高贵煤气的分析。我们介绍一个新奇煤气的纯化系统(中华人民共和国专利没有。ZL201320117751.2 ) ,它与不同吸收并且排除包括几可逆纯化泵温度。它能很好清理水蒸气,二氧化碳和器官的气体。云母矿物质经常为 40Ar/39 标明日期的 Ar。不能被正常准备系统与二 SAES NP10 ? getters 清理的一件白云母样品(2082MS ) ,在这研究为一个比较实验成为测试样品。实验与接着关上 / 打开的器官的煤气的移动系统(OGRS ) 被分配进 4 节。当 OGRS 为纯化仅仅与二 NP10 getters 被关上时, 40 因为杂质, Ar 紧张与入口时间在曲线增加, 40Ar/39 关于瑥潲潬楧慣?洠湩牥污杯捩污愠摮朠潥档浥捩污攠楶敤据 e 的 Ar | HE LiYan QIU HuaNing SHI HeSheng ZHU JunZhang BAI XiuJuan YUN JianBing | 2016 | Science China Earth Sciences2016，59，1： | 2 |
| 7 | Development of a real time PCR assay for rapid detection of Vibrio parahaemolyticus from seafood显示文摘A real time PCR assay for the detection of Vibrio para-haemolyticus in seafood samples was developed using a novel specific target and a competitive internal ampli-fication control(IAC).The specificity of this assay was evaluated using 390 bacterial strains including V.parahaemolyticus,and other strains belonging to Vibrio and non-Vibrio species.The real time PCR assay un-ambiguously distinguished V.parahaemolyticus with a detection sensitivity of 4.8 fg per PCR with purified genomic DNA or 1 CFU per reaction by counting V.parahaemolyticus colonies.The assays of avoiding interference demonstrated that,even in the presence of 2.1μg genomic DNA or 107 CFU background bacteria,V.parahaemolyticus could still be accurately detected.In addition,the IAC was used to indicate false-negative results,and lower than 94 copies of IAC per reaction had no influence on the detection limit.Ninety-six sea-food samples were tested,of which 58(60.4%)were positive,including 3 false negative results.Conse-quently,the real time PCR assay is effective for the rapid detection of V.parahaemotyticus contaminants in seafood. | Bin Liu Xiaohua He Wanyi Chen Shuijing Yu Chunlei Shi Xiujuan Zhou Jing Chen Dapeng Wang Xianming Shi | 2012 | Protein & Cell2012，3，3： | 2 |
| 8 | Scutel-larein inhibits cancer cell metastasis in vitro and attenu-ates the development of fibrosarcoma in vivo显示文摘 | Shi Xiujuan Chen Guangfeng Liu Xiaoqiang | 2015 | Int J MolMed2015，，： | 1 |
| 9 | Dynamic tabu search algorithm for solving departure scheduling problem 显示文摘 | WANG LAIJUN SHI ZHONGKE LEI XIUJUAN | 2007 | Journal of southwest jiotong University ( Endlish Edition)2007，15，2： | 1 |
| 10 | Evolution of composite fouling on a vertical stainless steel surface caused by treated sewage显示文摘 | Cheng Zan Lin Shi Wenyan Yang Xiujuan Ma | 2010 | Research article Front Energy Power Eng China 20102010，4，2： | 1 |
| 11 | Impact of long- range transport on aerosol properties at a regional background station in Northern China 显示文摘 | PU Weiwei ZHAO Xiujuan SHI Xuefeng | 2015 | Atmospheric Research2015，153，： | 1 |
| 12 | Low-temperature CO oxidation over supported Pt, Pd catalysts: Particular role of FeO x support for oxygen supply during reactions显示文摘 | Lequan Liu Feng Zhou Liguo Wang Xiujuan Qi Feng Shi Youquan Deng | 2010 | Journal of Catalysis2010，，1： | 1 |
| 13 | Manganese exposure among smelting workers: blood manganese–iron ratio as a novel tool for manganese exposure assessment显示文摘 | Dallas M. Cowan Qiyuan Fan Yan Zou Xiujuan Shi Jian Chen Michael Aschner Frank S. Rosenthal Wei Zheng | 2009 | Biomarkers2009，，1： | 1 |
| 14 | Generalized pigeon-inspired optimization algorithms显示文摘Dear editor,We propose a generalized pigeon-inspired optimization (GPIO) algorithm to enhance the exploitation ability of the original pigeon-inspired optimization (PIO) algorithm. Three variants of the PIO algorithms, including the original PIO,GPIO with ring structure (PIOr), and GPIO with ring structure and simplified landmark operator(PIOrs), are analyzed from a component-wise perspective. | Shi CHENG Xiujuan LEI Hui LU Yong ZHANG Yuhui SHI | 2019 | Science China(Information Sciences)2019，62，7： | 1 |
| 15 | One-step co-doping of ZnO and Zn^(2+)in osteoinductive calcium phosphate ceramics with synergistic antibacterial activity for regenerative repair of infected bone defect显示文摘How to endow bone grafts with long-term antibacterial activity and good bone regenerative ability to achieve the regenerative repair of infected bone defects has been the focus of the clinical treatment of osteomyelitis.The present study introduced a novel one-step route to realizing the co-doping of zinc oxide(ZnO)and zinc ion(Zn^(2+))in biphasic calcium phosphate(BCP)ceramics to utilize their synergistic antibacterial.Compared with the conventional BCP ceramics(BCP-Ca),the ZnO/Zn^(2+)co-doping ones(BCP-Zn)possessed strong antibacterial ability on E.coli and S.aureus as well as stimulated the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)effectively.The synergistic antibacterial mechanism of ZnO and Zn^(2+)was also investigated.BCP-Zn showed excellent osteoinductivity and angiogenesis at three months postoperatively in the canine intramuscular implantation model.Moreover,BCP-Zn exhibited excellent anti-infective ability and bone regenerative repair compared to BCP-Ca and control groups in the infected bone defect model of rat femur.Collectively,these findings suggest that the simultaneous introduction of ZnO/Zn^(2+)could have immense potential to expand the application of osteoinductive BCP ceramics in the regenerative repair of infected bone defects. | Tinghan He Hongxu Chen Puxin Liu Hao Shi Xiujuan Xu Cong Feng Yuyi Wang Xiangfeng Li Ning Lei Yumei Xiao Xiangdong Zhu Jianguo Xu Xingdong Zhang | 2023 | Journal of Materials Science & Technology2023，，32： | 1 |
| 16 | Effects of latitude gradient and seasonal variation on the community structure and biodiversity of commercially important crustaceans in the Yellow Sea and the northern East China Sea显示文摘To evaluate the spatio-temporal variations in the community structure and biodiversity of commercially important crustaceans in the Yellow Sea and the northern East China Sea(NECS),the seasonal and regional changes in species composition,biomass structure,biodiversity and distribution of commercially important species were analyzed using bottom trawl survey data during 2014-2015.The results showed that the latitudinal gradient was obvious in species richness,dominant species and biodiversity.The indices of biodiversity increased with the decreasing latitude.When the sampling sites shifted south by one latitudinal degree,Margalefs richness index(D),Pielou's evenness index(J')and Shannon-Wiener diversity index(H')increased by 0.10.0.03 and 0.09,respectively.The biomass proportion of the cold-temperate species represented by Crangon affinis declined with the decreasing latitude,and the warm-temperate species represented by Ovalipes punctatus and Portunus trituberculatus in creased.Because of the growth regulatio n of crustaceans and the fishing moratorium,the biomass of commercially important crustaceans in the Yellow Sea and NECS was highest in October and August,respectively.Salinity had a more significant influence on H'of commercially important crustaceans than other environmental factors(including zooplankton density,sea bottom temperature and water depth).Overall,the results of this study contribute to a better understanding of community dynamics of crustaceans in the Yellow Sea and NECS,and provide evidence to verify the latitudinal gradient theory in biodiversity. | Qiang Wu Xiujuan Shan Xianshi Jin Yue Jin Fangqun Dai Yongqiang Shi Lisha Guan Tao Zuo Jianqiang Sun | 2020 | Marine Life Science & Technology2020，2，2： | 0 |
| 17 | Evaporation and drying characteristics of the sessile ferrofluid droplet under a horizontal magnetic field显示文摘In this study,the evaporation characteristics and drying patterns of various sessile ferrofluid droplets on certain substrate under horizontal magnetic fields of controlled intensities are reported.The effects of droplet concentration and magnetic field intensity on the duration of each evaporation stage and drying patterns of droplets have been systematically investigated.It turned out that a plateau appears at the initial stage of evaporation in the absence of magnetic field and it was found that the plateau value is positively correlated with the concentration of ferrofluid droplets.Under the external magnetic field,the evaporation time of droplets decreases,the stage of contact line retreat extends,the stage of late pinning mode shortens,and the deposition area of ferrofluid droplet decreases compared to that of without magnetics field.The deposition area increases gradually and becomes more uniform with the increase of magnetic field.The decrease of friction force which is due to the decrease of the number of nanoparticles at the contact line under external magnetic field is the main reason for the observed phenomena.We found that the coffee ring and the uniform deposition inside the droplet will be destroyed when the magnetic field intensity is higher than a critical value.Our work has a significant reference value for the evaporation of sessile magnetic fluid droplets under the applied magnetic field,especially when the drying pattern needs to be precisely controlled,such as in spray or biomedicine. | Zhaonan Liu Jiandong Zhou Yang Li Xin Zhuo Xiujuan Shi Dengwei Jing | 2022 | Fundamental Research2022，2，2： | 0 |
| 18 | HAND2-AS1,PRKAA2 and VLDLR predict the risk of peritoneal metastasis in gastric cancer of different Lauren types based on STEPP analysis显示文摘The peritoneum is the most common site of recurrence of gastric cancer(GC).Early occult peritoneal metastasis is difficult to detect by imaging examination.Stratifying the risk of peritoneal metastasis in patients with different Lauren subtypes is of great clinical value.We performed a univariate Cox regression to identify those genes with prognostic value of overall survival(OS)and peritoneal-specified disease-free survival(psDFS)from the Gene Expression Omnibus database.The candidate genes were screened by the Subpopulation Treatment Effect Pattern Plot(STEPP)method.Propensity score matching(PSM)analysis was used to reduce the interference of confounders on the results.Based on the optimal cut-off values determined by the STEPP method,we found overexpression of three genes(HAND2-AS1,PRKAA2,and VLDLR)was correlated with shorter 1-year psDFS among patients with diffuse-type than that of patients with intestinal-type GC,and it is highly significant.Gene Set Enrichment Analysis(GSEA)potentially suggested that the three genes promote the early occurrence of peritoneal metastasis in patients with diffuse-type GC through glucose metabolism-related pathways.These three genes may be potential biomarkers.They can be used to assess the risk of peritoneal metastases to guide treatment decisions and follow-up strategies. | HUI QU RUICHUAN SHI BOWEN YANG XI CHEN YUJIA SONG LINGZI HE YITING SUN YUJING YANG ZHI LI XIUJUAN QU | 2022 | BIOCELL2022，46，3： | 0 |
| 19 | A pH-response chemotherapy synergistic photothermal therapy for tumor suppression and bone regeneration by mussel-inspired Mg implant显示文摘Primary malignant bone tumors can be life-threatening.Surgical resection of tumor plus chemotherapy is the standard clinical treatment.However,postoperative recovery is hindered due to tumor recurrence caused by residual tumor cells and bone defect caused by resection of tumor tissue.Herein,a multifunctional mussel-inspired film was fabricated on Mg alloy,that is,an inner hydrothermal-treated layer,a middle layer of polydopamine,and an outer layer of doxorubicin.The modified Mg alloy showed excellent photothermal effect and thermal/pH-controlled release of doxorubicin.The synergistic effect of chemotherapy and photothermal therapy enabled the modified Mg alloy to kill bone tumor in vitro and inhibit tumor growth in nude mice.Moreover,because of the controlled release of Mg ions and biocompatibility of polydopamine,the modified Mg alloy supported extracellular matrix mineralization,alkaline phosphatase activity,and bone-related gene expression in C3H10T1/2.Bone implantation model in rats verified that the modified Mg showed excellent osteointegration.These findings prove that the use of mussel-inspired multifunction film on Mg alloy offers a promising strategy for the therapy of primary malignant bone tumor. | Hongwei Shao Shi Cheng Mengyu Yao Xiongfa Ji Hua Zhong DonghuiWang Xiujuan Fan Qian Li Jielong Zhou Yu Zhang Feng Peng | 2021 | Regenerative Biomaterials2021，8，6： | 0 |
| 20 | Elite,transformable haploid inducers in maize显示文摘The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops. | Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli Rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu Yubo Liu Becky Breitinger Ana Poets Jason Nichols Wan Shi David Skibbe Qiudeng Que Timothy Kelliher | 2024 | The Crop Journal2024，12，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员