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| 1 | Carbon storage in evergreen broad-leaf forests in mid-subtropical region of China at four succession stages显示文摘To better understand the effect of forest succession on carbon sequestration, we investigated carbon stock and allocation of evergreen broadleaf forest, a major zonal forest in subtropical China.We sought to quantify the carbon sequestration potential.We sampled four forest types,shrub(SR), pine(Pinus massoniana) forest(PF), pine and broadleaf mixed forest(MF) and evergreen broadleaf forest(BF).A regression equation was constructed using tree height and diameter at breast height(DBH) and elements of total tree biomass.The equation was subsequently utilized to estimate tree carbon storage.The carbon storage of understory, litter, and soil was also estimated.Carbon storage in biomass increased significantly from the early succession stage SR(6.21 t ha-1) to the late stage BF(134.87 t ha-1).The biomass carbon stock of forest layers generally increased with succession except for the understory.The soil organic carbon storage for the total profile increased with forest succession, from 51.16 to 90.49 t ha-1, but the contribution of SOC to the carbon stock of the forest ecosystem declined from 89.18% to 40.15%.The carbon stock at ecosystem scale increased significantly with succession from SR(57.37 t ha-1), to PF(154.20 t ha-1), to MF(170.96 t ha-1)and to BF(225.36 t ha-1), with carbon stock of BF3.93 times that of SR.The forests in our study have great potential for increasing carbon sequestration, and large areas of secondary or degraded evergreen broadleaf forests in the subtropical zone of China could be a great carbon sink in future. | ZENG Zhangquan WANG Silong ZHANG Canming GONG Chao HU Qing | 2013 | Journal of Forestry Research2013，24，4： | 17 |
| 2 | Comparisons of carbon storages in Cunninghamia lanceolata and Michelia macclurei plantations during a 22-year period in southern China显示文摘Tree species composition was important for carbon storage within the same climate range.To quantify the dynamics of ecosystem carbon allocation as affected by different tree species,we measured the above-and below-ground biomass accumulation in 22 years,as well as the tissue carbon concentrations of trees in Cunninghamia lanceolata plantation and Michelia macclurei plantation.Results indicated that M.macclurei plantation significantly stored more carbon(174.8 tons/hm2) than C.lanceolata plantation(154.3 tons/hm2).Most of the carbon was found in the soil pool(57.1% in M.macclurei plantation,55.2% in C.lanceolata plantation).Tree and soil component of M.macclurei plantation possessed significantly higher carbon storage than that of C.lanceolata plantation(p < 0.05).No significant difference was found in the carbon storage of understory and forest floor.These results suggest that the broadleaved species(M.macclurei) possesses greater carbon sequestration potential than the coniferous species(C.lanceolata) in southern China. | NIU Dong WANG Silong OUYANG Zhiyun | 2009 | Journal of Environmental Sciences2009，21，6： | 15 |
| 3 | Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9显示文摘CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic T0 plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 sn RNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67%. Stable knockout transformants for maize phytoene synthase gene(PSY1) were obtained. Mutations occurred in germ cells can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize. | Jinjie Zhu Ning Song Silong Sun Weilong Yang Haiming Zhao Weibin Song Jinsheng Lai | 2016 | Journal of Genetics and Genomics2016，43，1： | 12 |
| 4 | Nitrogen Deposition and Its Spatial Pattern in Main Forest Ecosystems along North-South Transect of Eastern China显示文摘A continuous three-year observation(from May 2008 to April 2011)was conducted to characterize the spatial variation of dissolved inorganic nitrogen(DIN)deposition at eight main forest ecosystems along the north-south transect of eastern China(NSTEC).The results show that both throughfall DIN deposition and bulk DIN deposition increase from north to south along the NSTEC.Throughfall DIN deposition varies greatly from 2.7 kg N/(ha·yr)to 33.0 kg N/(ha·yr),with an average of 10.6 kg N/(ha·yr),and bulk DIN deposition ranges from 4.1 kg N/(ha·yr)to 25.4 kg N/(ha·yr),with an average of 9.8 kg N/(ha·yr).NH4+-N is the dominant form of DIN deposition at most sampling sites.Additionally,the spatial variation of DIN deposition is controlled mainly by precipitation.Moreover,in the northern part of the NSTEC,bulk DIN deposition is 17%higher than throughfall DIN deposition,whereas the trend is opposite in the southern part of the NSTEC.The results demonstrate that DIN deposition would likely threaten the forest ecosystems along the NSTEC,compared with the critical loads(CL)of N deposition,and DIN deposition in this region is mostly controlled by agricultural activities rather than industrial activities or transportation. | ZHAN Xiaoyun YU Guirui HE Nianpeng FANG Huajun JIA Bingrui ZHOU Mei WANG Chuankuan ZHANG Junhui ZHAO Guangdong WANG Silong LIU Yunfen YAN Junhua | 2014 | Chinese Geographical Science2014，24，2： | 10 |
| 5 | Wheat genomic study for genetic improvement of traits in China显示文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world. | Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong | 2022 | Science China(Life Sciences)2022，65，9： | 9 |
| 6 | Stability of soil organic carbon changes in successive rotations of Chinese fir(Cunninghamia lanceolata(Lamb.) Hook) plantations显示文摘The importance of soil organic carbon(SOC) under forests in the global carbon cycle depends on the stability of the soil carbon and its availability to soil microbial biomass.We investigated the effects of successive rotations of Chinese fir(Cunninghamia lanceolata(Lamb.) Hook) plantations on the stability of SOC and its availability to microbes by adopting the two-step hydrolysis with H2SO4 and density fractionation.The results showed that successive rotations of Chinese fir decreased the quantity of total SOC,recalcitrant fraction,and carbohydrates in Labile Pool I(LP Ⅰ),and microbial properties evidently,especially at 0-10 cm horizon.However,cellulose included in Labile Pool II(LP Ⅱ) and the cellulose/total carbohydrates ratio increased in successive rotations of Chinese fir.The non-cellulose of carbohydrates included in LP I maybe highly available to soil microbial biomass.Hence the availability of SOC to microbial biomass declined over the successive rotations.Although there was no significant change in recalcitrance of SOC over the successive rotations of Chinese fir,the percentage of heavy fraction to total SOC increased,suggesting that the degree of physical protection for SOC increased and SOC became more stable over the successive rotations.The degradation of SOC quality in successive rotation soils may be attributed to worse environmental conditions resulted from disturbance that related to 'slash and burn' site preparation.Being highly correlated with soil microbial properties,the cellulose/total carbohydrates ratio as an effective indicator of changes in availability of SOC to microbial biomass brought by management practices in forest soils. | ZHANG Jian WANG Silong FENG Zongwei WANG Qingkui | 2009 | Journal of Environmental Sciences2009，21，3： | 7 |
| 7 | Defective Kernel 39 encodes a PPR protein required for seed development in maize显示文摘RNA editing is a posttranscriptional process that is important in mitochondria and plastids of higher plants. All RNA editing-specific trans-factors reported so far belong to PLS-class of pentatricopeptide repeat(PPR)proteins. Here, we report the map-based cloning and molecular characterization of a defective kernel mutant dek39 in maize. Loss of Dek39 function leads to delayed embryogenesis and endosperm development, reduced kernel size, and seedling lethality. Dek39 encodes an E subclass PPR protein that targets to both mitochondria and chloroplasts, and is involved in RNA editing in mitochondrial NADH dehydrogenase3(nad3) at nad3-247 and nad3-275. C-to-U editing of nad3-275 is not conserved and even lost in Arabidopsis, consistent with the idea that no close DEK39 homologs are present in Arabidopsis. However, the amino acids generated by editing nad3-247 and nad3-275 are highly conserved in many other plant species, and the reductions of editing at these two sites decrease the activity of mitochondria NADH dehydrogenase complex I,indicating that the alteration of amino acid sequence is necessary for Nad3 function. Our results indicate that Dek39 encodes an E sub-class PPR protein that is involved in RNA editing of multiple sites and is necessary for seed development of maize. | Xiaojie Li Wei Gu Silong Sun Zongliang Chen Jing Chen Weibin Song Haiming Zhao Jinsheng Lai | 2018 | Journal of Integrative Plant Biology2018，60，1： | 6 |
| 8 | Soil microbial activity and nutrients of evergreen broad-leaf forests in mid-subtropical region of China显示文摘To better understand the effects of forest succession on soil microbial activity, a comparison of soil microbial properties and nutrients was conducted between three forest types representing a natural forest succession chronosequence. The study compared a pine(Pinus massoniana) forest(P_F), a pine and broadleaf mixed forest(M_F) and an evergreen broadleaf forest(B_F), in the Yingzuijie Biosphere Reserve, Hunan Province, China.Results showed that soil nutrients in the M_Fand BFplots were higher than in the P_Fplots. The range in microbial biomass carbon followed a similar pattern with B_Fhaving the greatest values, 522–1022 mg kg^-1, followed by M_F368–569 mg kg^-1, and finally, P_F193–449 mg kg^-1. Soil nutrients were more strongly correlated with microbial biomass carbon than basal respiration or metabolic quotient. Overall, forest succession in the study site improved soil microbial properties and soil fertility, which in turn can increase primary productivity and carbon sequestration. | Zhangquan Zeng Silong Wang Canming Zhang Hong Tang Xiquan Li Zijian Wu Jia Luo | 2015 | Journal of Forestry Research2015，26，3： | 5 |
| 9 | N concentration of old leaves and twigs is more sensitive to stand density than that of young ones in Chinese fir plantations: a case study in subtropical China显示文摘Young leaves are conventionally used in the analysis to study the nutrient status of evergreen plants and their responses to environmental changes, but the role of old leaves remains poorly understood. We selected two stand types in 31-year-old Chinese fir(Cunninghamia lanceolata) plantations with similar soil conditions but different stand densities, to test the hypothesis that nitrogen(N) concentration of old leaves and twigs is more sensitive to stand density than that of young ones. Leaves and twigs were sampled and sorted into young(one-year-old) and old(two-and three-year-old) groups. Significant differences in N concentration and carbon: nitrogen ratio between the low-density stand and high-density stand were only found in the old leaves and twigs but not in the young ones.Although the N resorption efficiency did not vary significantly with stand density, the annual N resorption rates were increased in old leaves and relatively young twigs at high stand density. These results show the potential use of old tissues in the nutrient analysis in Chinese fir plantations. Testing the generality of these results could improve the use of foliar analysis as an indicator of nutrient status and environmental changes in evergreen tree species. | Yuebao Di Xiaoli Fu Huimin Wang Wenhua Li Silong Wang | 2018 | Journal of Forestry Research2018，29，1： | 5 |
| 10 | fMRI time series analysis based on stationary wavelet and spectrum analysis显示文摘The low signal to noise ratio (SNR) of functional MRI (fMRI) prefers more sensitive data analysis methods. Based on stationary wavelet transform and spectrum analysis, a new method with high detective sensitivity was developed for analyzing fMRI time series, which does not require any prior assumption of the characteristics of noises. In the proposed method, every component of fMRI time series in the different time-frequency scales of stationary wavelet transform was discerned by the spectrum analysis, then the components from noises were removed using the stationary wavelet transform, finally the components of real brain activation were detected by cross-correlation analysis. The results obtained from both simulated and in vivo visual experiments illustrated that the proposed method has much higher sensitivity than the traditional cross-correlation method. | ZHI Lianhe ZHAO Xia SHAN Baoci PENG Silong YAN Qiang YUAN Xiuli TANG Xiaowei | 2006 | Progress in Natural Science:Materials International2006，16，11： | 3 |
| 11 | Fresh carbon and nitrogen inputs alter organic carbon mineralization and microbial community in forest deep soil layers显示文摘 | Qingkui Wang Yanping Wang Silong Wang Tongxin He Li Liu | 2014 | Soil Biology and Biochemistry2014，，： | 2 |
| 12 | Can Fluorine-18 Fluoroestradiol Positron Emission Tomography–Computed Tomography Demonstrate the Heterogeneity of Breast Cancer In Vivo?显示文摘 | Zhongyi Yang Yifei Sun Yongping Zhang Jing Xue Mingwei Wang Wei Shi Beiling Zhu Silong Hu Zhifeng Yao Herong Pan Yingjian Zhang | 2013 | Clinical Breast Cancer2013，，： | 2 |
| 13 | Carbon input manipulation affects soil respiration and microbial community composition in a subtropical coniferous forest显示文摘 | Qingkui Wang Tongxin He Silong Wang Li Liu | 2013 | Agricultural and Forest Meteorology2013，，： | 2 |
| 14 | Thermal behavior of an isolator with mode transition inducing back-pressure of a dual-mode scramjet显示文摘Combustion mode transition is a valuable and challenging research area in dual-mode scramjet engines.The thermal behavior of an isolator with mode transition inducing backpressure is investigated by direct-connect dual-mode scramjet experiments and theoretical analysis.Combustion experiments are conducted under the incoming airflow conditions of total temperature1270 K and Mach 2.A small increment of the fuel equivalence ratio is scheduled to trigger mode transition.Correspondingly,the variation of the coolant flow rate is very small.Based on the measured wall pressures,the heat-transfer model can quantify the thermal state variation of the engine with active cooling.Compared with the combustor,mode transition has a greater effect on the isolator thermal behavior,and it significantly changes the isolator heat-flux and wall temperature.To further study the isolator thermal behavior from flight Mach 4 to Mach 7,a theoretical analysis is carried out.Around the critical point of combustion mode transition,sudden changes of the isolator flowfield and thermal state are discussed. | Yang Qingchun Bao Wen Chetehouna Khaled Zhang Silong Gascoin Nicolas | 2017 | Chinese Journal of Aeronautics2017，30，2： | 2 |
| 15 | Real-Time H.265/HEVC Intra Encoding with a Configurable Architecture on FPGA Platform显示文摘This paper proposes a flexible design scheme for H.265/HEVC encoding based on FPGA, which allows an easy incorporation of a variety of algorithms applied to different scenarios. In particular, we present an H.265/HEVC intra encoder as an instantiation of our proposed scheme. The key idea is to develop an encoder system by configuring basic Processing elements(PEs) of fundamental algorithms. Our intra encoder using the flexible framework is structured with fourstage CTU based pipeline. Pixel-level PEs are designed to unify the intra prediction of 35 modes and a multiscale compatible transform array is proposed to process variable size transform. 32 PEs are paralleled for intra mode decision to support 35 combinations of modes and partitions. In the reconstruction stage, 16 PEs are paralleled for intra prediction and a 16 × 16 multiplier array is configured for transforms of variable sizes with a constant 16 pixels/cycle throughput. Implementation results show that our proposed architecture costs about63 K Lookup tables and 62 KB on-chip memories on Xilinx Kintex-7 platform with the maximum working frequency at 175 MHz, which is sufficient for real-time encoding of 1920 × 1080 @60 fps video at 160 MHz. The flexibility and extension capability of our framework provides a great potential for future FPGA solutions serving for different purposes. | DING Dandan WANG Silong LIU Zoe YUAN Qingshu | 2019 | Chinese Journal of Electronics2019，28，5： | 2 |
| 16 | 18F-FLT PET/CT imaging is not competent for the pretreatment evaluation of metastatic gastric cancer: a comparison with 18F-FDG PET/CT imaging显示文摘 | Min Zhou Chenchen Wang Silong Hu Yongping Zhang Zhifeng Yao Jin Li Weijian Guo Yingjian Zhang | 2013 | Nuclear Medicine Communications2013，，7： | 2 |
| 17 | Two Ultraviolet Radiation Datasets that Cover China显示文摘Ultraviolet(UV) radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization's measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. | Hui LIU Bo HU Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU | 2017 | Advances in Atmospheric Sciences2017，34，7： | 2 |
| 18 | Design and experiment of vertical pneumatic fertilization system with spiral Geneva mechanism显示文摘In the traditional fertilization method,a large amount of fertilizer is applied,which causes congestion easily.It is not conducive to the sustainable development of agriculture.In this work,a vertical pneumatic fertilization system with a spiral Geneva mechanism was designed according to the operational requirements of variable-rate and smooth fertilization.The uniformity in discharging fertilizer with different spiral angles was simulated and analyzed by extended discrete element method(EDEM)based simulation software,from where the spiral angle of the fertilizer discharge wheel was determined to be 45°.The fertilization system includes a fertilizer apparatus with a spiral Geneva mechanism,whose optimal performance parameters were obtained from the bench experiment.The accuracy and uniformity of the fertilizer application system were taken as the evaluation indicators.The linear relationships of the discharged amount of fertilizer with the rotational speed and the opening of the fertilizer discharging wheel were verified by both static blowing fertilization and field experiments.The static blowing experimental results show that the discharged amount of fertilizer has a high linear correlation and accuracy with both the opening and rotational speed,and the highest correlation occurs with the rotational speed.The rotational speed and opening have significant effects on the discharged amount of fertilizer and the average coefficient of variation.The effect of the rotational speed was the most significant.According to the model obtained by multiple regression fittings,the optimal parameters were determined when the average coefficient of variation was small,the rotational speed was 15.9 r/min and the opening was 34.4 mm.Therefore,the purpose of precise fertilization can be achieved by adjusting the opening or rotational speed in a way to get the exact amount of fertilizer discharged as required by the corresponding crops.The field experiment showed that the variation coefficient of each fertilizer discharged decreases first and then increases with an increasing opening under different rotational speeds,which is consistent with the theoretical value.When the opening was 40 mm or 50 mm,the variation coefficient reached the minimum value,which is far less than the qualified index of 7.8%.In the static blowing experiment and the dynamic field experiment,the stability of the fertilizer discharging device can be significantly enhanced with the utilization of the pneumatic conveying fertilizer.This study can provide a theoretical reference for parameter selection and optimization of vertical spiral fertilization systems. | Haibo Chen Jingfu Zheng Shaojie Lu Shan Zeng Silong Wei | 2021 | International Journal of Agricultural and Biological Engineering2021，14，4： | 2 |
| 19 | Thermodynamic analysis for a chemically recuperated scramjet显示文摘Endothermic hydrocarbon fuel is regarded as an optimal fuel for a scramjet with regenerative cooling,which provides extra cooling through endothermic chemical conversion to avoid the severly limited cooling capacity when conventional fuels are adopted for cooling.Although endothermic cooling is proposed from the view point that the heat sink of a conventional fuel is insufficient,the heat-absorbing through endothermic chemical reaction is actually a chemical recuperation process because the wasted heat dissipated from the engine thermal structure is recovered through the endothermic chemical reaction.Therefore,the working process of a scramjet with endothermic hydrocarbon fuel cooling is a chemical recuperative cycle.To analyze the chemical recuperative cycle of a chemically recuperated scramjet engine,we defined physical and chemical recuperation effectivenesses and heating value increment rate,and derived engine performance parameters with chemical recuperation.The heat value benefits from both physical and chemical recuperations,and it increases with the increase in recuperation effectiveness.The scramjet performance parameters also increase with the increase in chemical recuperation effectiveness.The increase in chemical recuperation effectiveness improves both the performances of the fuel cooling system and the combustion system.The results of analysis prove that the existence of a chemical recuperation process greatly improves the performance of the whole scramjet. | QIN Jiang BAO Wen ZHANG SiLong SONG YuFei YU DaRen | 2012 | Science China(Technological Sciences)2012，55，11： | 2 |
| 20 | A sequence-based genetic linkage map as a reference for Brassica rapa pseudochromosome assembly显示文摘 | Wang Yan Sun Silong Liu Bo | | 0，，： | 1 |
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