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| 1 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Single-cell transcriptomic landscape of human blood cells显示文摘High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis.However,an elaborate and comprehensive transcriptome reference of the whole blood system is lacking.Here,we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types,including hematopoietic stem cells,progenitors and mature blood cells derived from 21 healthy donors.With high sequencing depth and coverage,we constructed a single-cell transcriptional atlas of blood cells(ABC) on the basis of both protein-coding genes and long noncoding RNAs(lncRNAs),and showed a high consistence between them.Notably,putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified.While common transcription factor regulatory networks were activated in neutrophils and monocytes,lymphoid cells dramatically changed their regulatory networks during differentiation.Furthermore,we showed a subset of nucleated erythrocytes actively expressing immune signals,suggesting the existence of erythroid precursors with immune functions.Finally,a web portal offering transcriptome browsing and blood cell type prediction has been established.Thus,our work provides a transcriptional map of human blood cells at single-cell resolution,thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis. | Xiaowei Xie Mengyao Liu Yawen Zhang Bingrui Wang Caiying Zhu Chenchen Wang Qing Li Yingying Huo Jiaojiao Guo Changlu Xu Linping Hu Aiming Pang Shihui Ma Una Wang Wenbin Cao Shulian Chen Qiuling Li Sudong Zhang Xueying Zhao Wen Zhou Hongbo Luo Guoguang Zheng Erlie Jiang Sizhou Feng Lixiang Chen Lihong Shi Hui Cheng Sha Hao Ping Zhu Tao Cheng | 2021 | National Science Review2021，8，3： | 3 |
| 3 | Multifunctional dendrimer-entrapped gold nanoparticles for dual mode CT/MR imaging applications显示文摘 | Shihui Wen Kangan Li Hongdong Cai Qian Chen Mingwu Shen Yunpeng Huang Chen Peng Wenxiu Hou Meifang Zhu Guixiang Zhang Xiangyang Shi | 2013 | Biomaterials2013，，5： | 1 |
| 4 | Fast MRI Reconstruction via Edge Attention显示文摘Fast and accurate MRI reconstruction is a key concern in modern clinical practice.Recently,numerous Deep-Learning methods have been proposed for MRI reconstruction,however,they usually fail to reconstruct sharp details from the subsampled k-space data.To solve this problem,we propose a lightweight and accurate Edge Attention MRI Reconstruction Network(EAMRI)to reconstruct images with edge guidance.Specifically,we design an efficient Edge Prediction Network to directly predict accurate edges from the blurred image.Meanwhile,we propose a novel Edge Attention Module(EAM)to guide the image reconstruction utilizing the extracted edge priors,as inspired by the popular self-attention mechanism.EAM first projects the input image and edges into Q_(image),K_(edge),and V_(image),respectively.Then EAM pairs the Q_(image)with K_(edge)along the channel dimension,such that 1)it can search globally for the high-frequency image features that are activated by the edge priors;2)the overall computation burdens are largely reduced compared with the traditional spatial-wise attention.With the help of EAM,the predicted edge priors can effectively guide the model to reconstruct high-quality MR images with accurate edges.Extensive experiments show that our proposed EAMRI outperforms other methods with fewer parameters and can recover more accurate edges. | Hanhui Yang Juncheng Li Lok Ming Lui Shihui Ying Jun Shi Tieyong Zeng | 2023 | Communications in Computational Physics2023，33，5： | 0 |
| 5 | Efficient Pd-Catalyzed C-H Oxidative Bromination of Arenes with Dimethyl Sulfoxide and Hydrobromic Acid显示文摘of main observation and conclusion We have developed an efficient Pd-catalyzed directed C-H bromination protocol,in which dimethyl sulfoxide(DMSO)is employed as oxidant with hydrobromic add aqueous solution(HBr(aq))as bromide source.The DMSO/HBr(aq)system,which is novelly and efficiently utilized in transition-metal catalyzed C-H activation,illustrates its practicability by the operational simplicity,inexpensive and readily available starting materials,and high bromide-atom economy. | Yizhi Yuan Yujie Liang Shihui Shi Yu-Feng Liang Ning Jiao | 2020 | Chinese Journal of Chemistry2020，38，11： | 0 |
| 6 | A large-scale screening of metal-organic frameworks for iodine capture combining molecular simulation and machine learning显示文摘We performed large-scale molecular simulation to screen and identify metal-organic framework materials for gaseous iodine capture,as part of our ongoing effort in addressing management and handling issues of various radionuclides in the grand scheme of spent nuclear fuel reprocessing.Starting from the computation-ready experimental(CoRE)metal-organic frameworks(MOFs)database,grand canonical Monte Carlo simulation was employed to predict the iodine uptake values of the MOFs.A ranking list of MOFs based on their iodine uptake capabilities was generated,with the Top 10 candidates identified and their respective adsorption sites visualized.Subsequently,machine learning was used to establish structure-property relationships to correlate MOFs’various structural and chemical features with their corresponding performances in iodine capture,yielding interpretable common features and design rules for viable MOF adsorbents.The research strategy and framework of the present study could aid the development of high-performing MOF adsorbents for capture and recovery of radioactive iodine,and moreover,other volatile environmentally hazardous species. | Min Cheng Zhiyuan Zhang Shihui Wang Kexin Bi Kong-qiu Hu Zhongde Dai Yiyang Dai Chong Liu Li Zhou Xu Ji Wei-qun Shi | 2023 | Frontiers of Environmental Science & Engineering2023，17，12： | 0 |
| 7 | A high-quality,phased genome assembly of broomcorn millet reveals the features of its subgenome evolution and 3D chromatin organization显示文摘Dear Editor,Grasses of the genus Panicumgrowin natural and agricultural ecosystems worldwide and include about 450 species distributed throughout tropical and temperate regions.Most Panicumgrasses remain unexploited,with the exceptions of broomcorn millet(P.miliaceum)(Shi et al.,2019;Zou et al.,2019),switchgrass(P.virgatum)(Lovell et al.,2021),Hall’s panicgrass(P.hallii)(Lovell et al.,2018),and a few other species that have been successfully domesticated into staple,forage,and energy crops.Broomcorn millet(2n=4x=36)is probably one of the earliest domesticated grain crops,originating in North China around 10000 years ago. | Zhiheng Wang Shihui Huang Zhengyue Yang Jinsheng Lai Xiang Gao Junpeng Shi | 2023 | Plant Communications2023，4，3： | 0 |
| 8 | Atom Recombination of Difluorocarbene Enables 3-Fluorinated Oxindoles from 2-Aminoarylketones显示文摘Contrary to the traditional implementation as a difluoromethyl group and recently disclosed role of C1 synthons in synthetic organic chemistry,difluorocarbene(:CF_(2))is reported herein to proceed in unprecedented atom recombination as both a C1 synthon and F1 reagent simultaneously to render valuable 3-fluorinated oxindoles from 2-aminoarylketones.The reaction does not require catalyst and features a broad range of substrates with good functional group compatibility and ease of execution.This transformation could be employed to the quick-constructions of certain bioactive molecule derivatives.The mechanistic experiments and density functional theory(DFT)calculations indicate that this atom recombination reaction of:CF_(2) for the synthesis of 3-fluorinated oxindoles may involve a rearrangement process of epoxide intermediates. | Guan Zhang Qianqian Shi Mengyuan Hou Kai Yang Shihui Wang Shuai Wang Wangyang Li Chaokun Li Jian Qiu Hetao Xu Lu Zhou Cece Wang Shi-Jun Li Yu Lan Qiuling Song | 2022 | CCS Chemistry2022，4，5： | 0 |
| 9 | Inhibition of aryl hydrocarbon receptor signaling promotes the terminal differentiation of human erythroblasts显示文摘The aryl hydrocarbon receptor(AHR)plays an important role during mammalian embryo development.Inhibition of AHR signaling promotes the development of hematopoietic stem/progenitor cells.AHR also regulates the functional maturation of blood cells,such as T cells and megakaryocytes.However,little is known about the role of AHR modulation during the development of erythroid cells.In this study,we used the AHR antagonist StemRegenin 1(SR1)and the AHR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin during different stages of human erythropoiesis to elucidate the function of AHR.We found that antagonizing AHR signaling improved the production of human embryonic stem cell derived erythrocytes and enhanced erythroid terminal differentiation.RNA sequencing showed that SR1 treatment of proerythroblasts upregulated the expression of erythrocyte differentiation-related genes and downregulated actin organization-associated genes.We found that SR1 accelerated F-actin remodeling in terminally differentiated erythrocytes,favoring their maturation of the cytoskeleton and enucleation.We demonstrated that the effects of AHR inhibition on erythroid maturation were associated with F-actin remodeling.Our findings help uncover the mechanism for AHRmediated human erythroid cell differentiation.We also provide a new approach toward the large-scale production of functionally mature human pluripotent stem cell-derived erythrocytes for use in translational applications. | Yijin Chen Yong Dong Xulin Lu Wanjing Li Yimeng Zhang Bin Mao Xu Pan Xiaohong Li Ya Zhou Quanming An Fangxin Xie Shihui Wang Yuan Xue Xinping Cai Mowen Lai Qiongxiu Zhou Yan Yan Ruohan Fu Hong Wang Tatsutoshi Nakahata Xiuli An Lihong Shi Yonggang Zhang Feng Ma | 2022 | Journal of Molecular Cell Biology2022，14，2： | 0 |
| 10 | Advances in regenerative medicine applications of tetrahedral framework nucleic acid-based nanomaterials: an expert consensus recommendation显示文摘With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids(t FNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, t FNAs have been widely applied in the biomedical field as threedimensional DNA nanomaterials. Surprisingly, t FNAs exhibit positive effects on cellular biological behaviors and tissue regeneration,which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity,t FNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization,intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic t FNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic t FNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone,cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases. | Yunfeng Lin Qian Li Lihua Wang Quanyi Guo Shuyun Liu Shihui Zhu Yu Sun Yujiang Fan Yong Sun Haihang Li Xudong Tian Delun Luo Sirong Shi | 2022 | International Journal of Oral Science2022，14，4： | 0 |
| 11 | Lateral damage mechanism of the double-track tunnel in the mountainous layered soft rock and NPR anchor cable control显示文摘Due to complex geological formations,lateral damage often occurs during excavation in mountainous layered soft rock double-track tunnels.This paper discusses the stresses and the damage characteristics of the surrounding rock under overload in a mountainous layered soft rock double-track tunnel through indoor model experiments to provide a basis for the effective control of lateral damage.The experiments show that the conventional support method cannot effectively control the lateral damage due to interlayer sliding.Therefore,the negative Poisson’s ratio(NPR)anchor/cable control method is proposed.And the scientificity and feasibility of the NPR anchor/cable control technology are proved by the field application and monitoring data of the Minxian Tunnel and Changning Tunnel.It is further demonstrated that high preload is the most effective way to control the lateral damage in layered soft rock tunnels. | Qiru Sui Manchao He Tingting Shi Shihui Pang Jinyang Xiang Zhigang Tao Dingjun Qu | 2023 | Underground Space2023，，5： | 0 |
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