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| 1 | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry显示文摘The aim of this work was to develop an automated on-line solid phase extraction(SPE)with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples.Samples were extracted with 0.2%formic acid acetonitrile solution,purified by on-line SPE device with HLB column,then separated by XBridge C18 column,using 0.1%formic acid solution and acetonitrile as the mobile phase.Mass spectrometric data was acquired under multiple reaction monitoring(MRM)mode using positive ionization electrospray.Internal standard method was used in the quantification,good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990.The limits of detection were in the range of 0.125–2.00g/kg and the limits of quantitation were in the range of 0.250–5.00g/kg.Recoveries of the method were in range of 78.3%–99.3%,relative standard deviation were lower than 10%.The method was simple,sensitivity,and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish. | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li | 2020 | Food Science and Human Wellness2020，9，4： | 6 |
| 2 | Immobilization of -galactosidase from Cier arietinum (gram chicken bean)and its catalytic actions显示文摘 | Weixia Tu Sufang Sun Shaoli Nu | 1999 | Food Chemistry1999，64，： | 1 |
| 3 | EBV encoded miR-BHRF1-1 potentiates viral lytic replication by downregulating host p53 in nasopharyngeal carcinoma显示文摘 | Zijian Li Xue Chen Lili Li Sufang Liu Lifang Yang Xiaoqian Ma Min Tang Ann M. Bode Zigang Dong Lunquan Sun Ya Cao | 2011 | International Journal of Biochemistry and Cell Biology2011，，2： | 1 |
| 4 | CO_(2)methanation boosted by support-size-dependent strong metal-support interaction and B-O-Ti component显示文摘Strong metal-support interaction(SMSI)has a great impact on the activity and selectivity of heterogeneous catalysts,which was usually adjusted by changing reduction temperature or processing catalyst in different atmosphere.However,few researches concentrate on modulating SMSI through regulating the structure of the support.Herein,we show how changing the surface environment of the anatase TiO_(2)(B–TiO_(2))can be used to modulate the SMSI.The moderate TiOx overlayer makes the Ni metal highly dispersed on the high specific surface area of support,resulting in a substantially enhanced CO_(2)methanation rate.Besides,a novel phenomenon was observed that boron dopants promote the for-mation of the B–O–Ti interface site,enhancing the catalytic performance of CO_(2)hydrogenation.DFT calculations confirm that the B–O–Ti structure facilitates the activation of CO_(2)and further hydrogenation to methane. | Shaoyu Yuan Yushan Yang Zhangyi Xiong Peijing Guo Sufang Sun Zejiang Li Jianlong Du Yongjun Gao | 2024 | Green Energy & Environment2024，9，2： | 0 |
| 5 | Molecular characterization and expression of HSP70, HSF and HSBP genes in Octopus vulgaris during thermal stress显示文摘Temperature is an important environmental factor that affects the growth and survival of Octopus vulgaris, the common octopus. To understand the protective mechanism that O. vulgaris exhibits under heat stress, we used rapid amplification of c DNA ends(RACE) to obtain full-length sequences of three heat stress response related genes:(1) the heat shock protein 70(OvHSP70),(2) the heat shock transcription factor(OvHSF), and(3) the heat shock factor-binding protein(OvHSBP) of O. vulgaris. The OvHSP70, OvHSF, and OvHSBP proteins contained2 222 bp, 2 264 bp, 841 bp that encoded for 635, 458 and 90 amino acids, respectively. The results of multiple sequence alignment showed that the amino acid sequences of OvHSP70 were highly conserved with respect to other species. Similarly, the DNA binding domain, the trimerization domain of OvHSF, and the coiled coil region of OvHSBP also had highly conserved regions. The real-time polymerase chain reaction(PCR) results indicated that OvHSP70 was temperature-dependent and time-dependent, showing a positive response to heat stress. On exposure to 28°C and to 30°C, the m RNA expression levels of OvHSF and OvHSBP were higher than those in the control group at 24°C. The m RNA expression of OvHSBP significantly increased with heat treatment at 26°C, while the m RNA expression of OvHSF decreased. The experimental results indicated that the expression of OvHSP70,OvHSF and OvHSBP were all sensitive to heat stress, which suggests that these three genes may play an important role for O. vulgaris in responding to environmental stress. Thus, this study sets a theoretical foundation for further in-depth studies on the molecular protective mechanisms of the heat response in O. vulgaris. | HONG Jingni MAO Yong NIU Sufang SUN Tiantian SU Yongquan | 2015 | Acta Oceanologica Sinica2015，34，8： | 0 |
| 6 | Breeding and Evaluation of New Iron Yam (Dioscorea opposita Thunb) Varieties显示文摘[Objective] This study was conducted to develop some new iron yam(Dioscorea opposita) varieties with excellent cooking, eating and nutritional qualities.[Method] The fresh weight per plant, drying rate, dry weight per plant, yield, allantoin content, water soluble extract content and disease resistance of seven new iron yam cultivars(No.1, No.2, No.4, No.6, No.8, No.9 and No.10) obtained through spaceflight mutation breeding were measured and compared with control(No.11).Then, the nutrition quality of two elite cultivars No. 6 and No.10 was determined by comparing with the control. [Result] The eight iron yam cultivars ranked in descending order of their fresh weight per plant as follows: No.6(0.255 kg)>No.10(0.254 kg)>No.4(0.242 kg)>No.9(0.237 kg)>No.1(0.233 kg)>No.11(0.206 kg)>No.2(0.191 kg)>No.8(0.157 kg). There was no significant difference in fresh weight per plant between No.6 and No.10, but extremely significant difference between No.6 and the control,No.10 and the control. The eight iron yam cultivars ranked in descending order of their drying rate as follows: No.2(32.641%)>No.10(32.230%)>No.9(28.223%)>No.6(25.174%)>No.8(25.122%)>No.11(25.043%)>No.1(24.291%)>No.4(20.234%). The drying rate of both No.6 and No.10 was higher than that of the control. The eight iron yam cultivars ranked in descending order of their dry weight per plant as follows:No.10(0.082 kg)>No.9(0.067 kg)>No.6(0.064 kg)>No.2(0.062 kg)>No.1(0.056 kg)>No.11(0.052 kg)>No.4(0.049 kg)>No.8(0.039 kg). The dry weight per plant of both No.6and No.10 is higher than that of the control. The eight iron yam cultivars ranked in descending order of their allantoin content as follows No.6(0.484%)>No.4(0.467%)>No.10(0.399%) >No.11(0.386%) >No.9(0.378%) >No.8(0.350%) >No.2(0.342) >No.1(0.325%). The allantoin content of No.6 was significantly higher than that of No.10 and the control. There was significant difference in allantoin content between No.6and No.10, but no significant difference between No 10 and the control. The eight iron yam cultivars ranked in descending order of their extract content as follows No.6(20.49%)>No.2(16.01%)>No.4(15.54%)>No.10(15.35%)>No.1(14.48%)>No.11(14.10%)>No.9(13.88%)>No.8(11.87%). The extract content of No.6 exhibited extremely significant difference with that of No.10 and the control, and the extract content of No.10 was also significantly different from that of the control. The taste of No.10 was dry, soft, floury, sweet and fragrant, better than that of the other seven cultivars.No.6 had the strongest resistance to Gloeosporium pestis and Cylindrosporium dioscoreae. No.10 had a middle resistance to G. pestis and a strong resistance to C. dioscoreae. No.6 and No.10 had higher contents of starch, reducing sugar, protein and ash, lower water content and better nutrition quality than the control.[Conclusion] No.10 can be popularized as a new variety of edible iron yam, and No.6as a new variety of medicinal iron yam. | Sufang DUAN Jianjun LI Meiling REN Jun WANG Guolun JIA Shuwu SUN | 2015 | Agricultural Science & Technology2015，16，10： | 0 |
| 7 | An index system for evaluating children reading promotion programs in public libraries显示文摘Reading program is an important way to cultivate children’s reading habits and interest.This study aims at exploring how to assess the effectiveness and impact of children’s reading programs held by public libraries.On the basis of related research,through applying the Delphi method and the analytic hierarchy process,this study constructs a multi-dimensional evaluation system for children’s reading programs from the perspectives of library,users and society.This system is characterized by covering criteria on input and output,outcomes and impact.Indicators on library input and the benefits for participants are considered more important. | Sufang WANG Yunqian SUN Bo WANG | 2014 | Journal of Library Science in China2014，，1： | 0 |
| 8 | Determination of L-ergothioneine in food by UPLC-MS/MS method显示文摘L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the quantitative determination of L-EGT in food.The sample was extracted with methanol-water(70:30,V/V),separated by hydrophilic interaction liquid chromatography(HILIC)and detected by triple-quadrupole mass spectrometry.Validation studies were carried out on different product and the limit of quantitation was 20μg/kg(milk,alcohol-free beverages,dairy products)and 40µg/kg(cereal bars,chocolate).Excellent linearity(correlation coefficient(R2)≥0.999)was achieved for L-EGT quantification in the range of 5–200 ng/mL.The recoveries of the method(83.7%−107.5%)and the relative standard deviation(RSD,0.88%−6.84%(n=6))meet the performance criteria required for the determination of L-EGT in food.Finally,the applicability of the method was tested by analysing actual samples.In general,the method developed is simple,reliable,accurate,and stable and could be useful for routine analyses of L-EGT in food. | Hongwen Zhai Lei Sun Sufang Fan Jing Wang Meirong Cao Hongyu Ma Qiang Li Yan Zhang | 2023 | Journal of Future Foods2023，3，2： | 0 |
| 9 | Differential regulation of JAK1 expression by ETS1 associated with predisposition to primary biliary cholangitis显示文摘Primary biliary cholangitis(PBC)is an autoimmune liver disease characterized by the destruction of intrahepatic small bile ducts and progressive cholestasis,eventually leading to liver cirrhosis and hepatic failure without appropriate treatment(Terziroli Beretta-Piccoli et al.,2019). | Peng Jiang Chan Wang Mingming Zhang Ye Tian Weifeng Zhao Junyi Xin Yexi Huang Zhibin Zhao Wenjuan Sun Jie Long Ruqi Tang Fang Qiu Xingjuan Shi Yi Zhao Li Zhu Na Dai Lei Liu Xudong Wu Jinshan Nie Bo Jiang Youlin Shao Yueqiu Gao Jianjiang Yu Zhigang Hu Zhidong Zang Yuhua Gong Yaping Dai Lan Wang Ningling Ding Ping Xu Sufang Chen Lu Wang Jing Xu Luyao Zhang Junyan Hong Ruonan Qian Hu Li Xuan Jiang Congwei Chen Wenyan Tian Jian Wu Yuzhang Jiang Chongxu Han Kui Zhang Hong Qiu Li Li Hong Fan Liming Chen Jianqiong Zhang Zhongsheng Sun Xiao Han Zhenhua Dai Erguang Li M.Eric Gershwin Zhexiong Lian Xiong Ma Michael F.Seldin Weichang Chen Meilin Wang Xiangdong Liu | 2023 | Journal of Genetics and Genomics2023，50，10： | 0 |
| 10 | Expression of Fragaria ananassa Osmotin-like Protein(FaOLP2) in E. coli:Purification and Antifungal Activity显示文摘[Objective]The FaOLP2(Fragaria ananassa osmotin-like protein)is a functional homolog of PR5-like protein.This study was undertaken to produce recombinant FaOLP2 and to identify its antifungal activity.[Method]The ORF of FaOLP2(accession number DQ325524)was cloned into pET22b vector to construct the pET22b-FaOLP2 plasmid.The recombinant mature FaOLP2 was expressed in E.coli Rosetta-gami B(DE3)by inducing with 1 mmol/L IPTG and found exclusively in insoluble inclusion bodies.As FaOLP2 requires the correct formation of eight disulfide bonds,but there were no obvious effect to correctly form these by expression at different temperatures and high osmotic pressure(supplemented Betaineand and D-Sorbitol),we used an in vitro method to refold E.coli expressed FaOLP2 by gradually elution using reduced:oxidized gluthatione redox buffer,followed by 8 mol/L urea solubilized His6-tagged mature FaOLP2 protein,which was affinity-purified by an immobilized-metal(Ni2+)affinity chromatography(IMAC)column.[Result]This method generated biologically active conformations of the recombinant mature FaOLP2 that displayed antifungal activity against Ustilaginoides virens,a plant pathogenic fungus,which causes rice false smut.[Conclusion]This study laid the foundation for further biotechnological application of the novel protein. | Bai LI Sufang LI Huandi LI Junwei SUN Keqin ZOU | 2013 | Agricultural Biotechnology2013，2，6： | 0 |
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