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| 1 | Allyl isothiocyanate ameliorates lipid accumulation and inflammation in nonalcoholic fatty liver disease via the Sirt1/AMPK and NF-κB signaling pathways显示文摘BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct impact on the liver.AIM To investigate the effect and underlying mechanism of AITC in nonalcoholic fatty liver disease(commonly referred to as NAFLD).METHODS To establish a mouse and cellular model of NAFLD,C57BL/6 mice were fed a high fat diet(HFD)for 8 wk,and AML-12 cells were treated with 200μM palmitate acid for 24 h.For AITC treatment,mice were administered AITC(100 mg/kg/d)orally and AML-12 cells were treated with AITC(20μmol/L).RESULTS AITC significantly ameliorated HFD-induced weight gain,hepatic lipid accumulation and inflammation in vivo.Furthermore,serum alanine aminotransferase and aspartate aminotransferase levels were markedly reduced in AITC-treated mice.Mechanistically,AITC significantly downregulated the protein levels of sterol regulatory elementbinding protein 1(SREBP1)and its lipogenesis target genes and upregulated the levels of proteins involved in fatty acidβ-oxidation,as well as the upstream mediators Sirtuin 1(Sirt1)and AMPactivated protein kinaseα(AMPKα),in the livers of HFD-fed mice.AITC also attenuated the nuclear factor kappa B(NF-κB)signaling pathway.Consistently,AITC relieved palmitate acid-induced lipid accumulation and inflammation in AML-12 cells in vitro through the Sirt1/AMPK and NF-κB signaling pathways.Importantly,further studies showed that the curative effect of AITC on lipid accumulation was abolished by siRNA-mediated knockdown of either Sirt1 or AMPKαin AML-12 cells.CONCLUSION AITC significantly ameliorates hepatic steatosis and inflammation by activating the Sirt1/AMPK pathway and inhibiting the NF-κB pathway.Therefore,AITC is a potential therapeutic agent for NAFLD. | Chun-Xiao Li Jian-Guo Gao Xing-Yong Wan Yi Chen Cheng-Fu Xu Ze-Min Feng Hang Zeng Yi-Ming Lin Han Ma Ping Xu Chao-Hui Yu You-Ming Li | 2019 | World Journal of Gastroenterology2019，25，34： | 14 |
| 2 | Flunarizine inhibits sensory neuron excitability by blocking voltage-gated Na+ and Ca2+ currents in trigeminal ganglion neurons显示文摘背景尽管 flunarizine 广泛地与清楚的成功为周期性偏头痛预防被使用了,它在周期性偏头痛预防的行动的机制不是完全这研究的 understood.The 目的是在tetrodotoxin抵抗的Na+隧道和尖锐地孤立的老鼠的高电压的激活的Ca2+隧道上调查 flunarizine 的效果三叉的在三叉的中心神经原的中心 neurons.Methods 钠水流和钙水流用整个房间的补丁夹钳 recordin | YE Qing WANG Qiang YAN Lan-yun WU Wen-hui LIU Sha XIAO Hang WAN Qi | 2011 | Chinese Medical Journal2011，，17： | 7 |
| 3 | Clinical applications of exosome membrane proteins显示文摘Extracellular vesicles(EVs)are small membranous particles that can mediate cell-to-cell communication and which are divided into at least three categories according to their subcellular origin and size:exosomes,microvesicles,and apoptotic bodies.Exosomes are the smallest(30–150 nm)of these EVs,and play an important role in EV-mediated cell-to-cell interactions,by transferring proteins,nucleic acids and,lipids from their parental cells to adjacent or distant cells to alter their phenotypes.Most exosome studies in the past two decades have focused on their nucleic acid composition and their transfer ofmRNAs and microRNAs to neighboring cells.However,exosomes also carry specific membrane proteins that can identify the physiological and pathological states of their parental cells or indicate their preferential target cells or tissues.Exosome membrane protein expression can also be directly employed or modified to allow exosomes to serve as drug delivery systems and therapeutic platforms,including in targeted therapy approaches.This review will briefly summarize information on exosome membrane proteins components and their role in exosome–cell interactions,including proteins associated with specific cell-interactions and diseases,and the potential for using exosome membrane proteins in therapeutic targeting approaches. | Qian Hu Hang Su Juan Li Christopher Lyon Wenfu Tang Meihua Wan Tony Ye Hu | 2020 | Precision Clinical Medicine2020，3，1： | 6 |
| 4 | Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material显示文摘Stem cell-based therapy has been used to treat ischaemic heart diseases for two decades.However,optimal cell types and transplantation methods remain unclear.This study evaluated the therapeutic effects of human umbilical cord mesenchymal stem cell(hUCMSC)sheet on myocardial infarction(MI).Methods:hUCMSCs expressing luciferase were generated by lentiviral transduction for in vivo bio-luminescent imaging tracking of cells.We applied a temperature-responsive cell culture surface-based method to form the hUCMSC sheet.Cell retention was evaluated using an in vivo bio-luminescent imaging tracking system.Unbiased transcriptional profiling of infarcted hearts and further immunohistochemical assessment of monocyte and macrophage subtypes were used to determine the mechanisms underlying the therapeutic effects of the hUCMSC sheet.Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function,angiogenesis and left ventricular remodelling.Results:When transplanted to the infarcted mouse hearts,hUCMSC sheet significantly improved the retention and survival compared with cell suspension.At the early stage of MI,hUCMSC sheet modulated inflammation by decreasing Mcp1-positive monocytes and CD68-positive macrophages and increasing Cx3cr1-positive non-classical macrophages,preserving the cardiomyocytes from acute injury.Moreover,the extracellular matrix produced by hUCMSC sheet then served as bioactive scaffold for the host cells to graft and generate new epicardial tissue,providing mechanical support and routes for revascularsation.These effects of hUCMSC sheet treatment significantly improved the cardiac function at days 7 and 28 post-MI.Conclusions:hUCMSC sheet formation dramatically improved the biological functions of hUCMSCs,mitigating adverse post-MI remodelling by modulating the inflammatory response and providing bioactive scaffold upon transplantation into the heart.Translational perspective:Due to its excellent availability as well as superior local cellular retention and survival,allogenic transplantation of hUCMSC sheets can more effectively acquire the biological functions of hUCMSCs,such as modulating inflammation and enhancing angiogenesis.Moreover,the hUCMSC sheet method allows the transfer of an intact extracellular matrix without introducing exogenous or synthetic biomaterial,further improving its clinical applicability. | Rui Guo Feng Wan Masatoshi Morimatsu Qing Xu Tian Feng Hang Yang Yichen Gong Shuhong Ma Yun Chang Siyao Zhang Youxu Jiang Heqing Wang Dehua Chang Hongjia Zhang Yunpeng Ling Feng Lan | 2021 | Bioactive Materials2021，6，9： | 6 |
| 5 | Intermetallic Cu_(5)Zr Clusters Anchored on Hierarchical Nanoporous Copper as Efficient Catalysts for Hydrogen Evolution Reaction显示文摘Designing highly active and robust platinum-free electrocatalysts for hydrogen evolution reaction is vital for large-scale and efficient production of hydrogen through electrochemical water splitting.Here,we report nonprecious intermetallic Cu_(5)Zr clusters that are in situ anchored on hierarchical nanoporous copper(NP Cu/Cu_(5)Zr)for efficient hydrogen evolution in alkaline medium.By virtue of hydroxygenated zirconium atoms activating their nearby Cu-Cu bridge sites with appropriate hydrogenbinding energy,the Cu_(5)Zr clusters have a high electrocatalytic activity toward the hydrogen evolution reaction.Associated with unique architecture featured with steady and bicontinuous nanoporous copper skeleton that facilitates electron transfer and electrolyte accessibility,the self-supported monolithic NP Cu/Cu_(5)Zr electrodes boost violent hydrogen gas release,realizing ultrahigh current density of 500mAcm^(-2) at a low potential of-280mV versus reversible hydrogen electrode,with exceptional stability in 1M KOH solution.The electrochemical properties outperform those of state-of-the-art nonprecious metal electrocatalysts and make them promising candidates as electrodes in water splitting devices. | Hang Shi Yi-Tong Zhou Rui-Qi Yao Wu-Bin Wan Qing-Hua Zhang Lin Gu Zi Wen Xing-You Lang Qing Jiang | 2020 | Research2020，，1： | 4 |
| 6 | Self-Powered Intelligent Human-Machine Interaction for Handwriting Recognition显示文摘Handwritten signatures widely exist in our daily lives.The main challenge of signal recognition on handwriting is in the development of approaches to obtain information effectively.External mechanical signals can be easily detected by triboelectric nanogenerators which can provide immediate opportunities for building new types of active sensors capable of recording handwritten signals.In this work,we report an intelligent human-machine interaction interface based on a triboelectric nanogenerator.Using the horizontal-vertical symmetrical electrode array,the handwritten triboelectric signal can be recorded without external energy supply.Combined with supervised machine learning methods,it can successfully recognize handwritten English letters,Chinese characters,and Arabic numerals.The principal component analysis algorithm preprocesses the triboelectric signal data to reduce the complexity of the neural network in the machine learning process.Further,it can realize the anticounterfeiting recognition of writing habits by controlling the samples input to the neural network.The results show that the intelligent human-computer interaction interface has broad application prospects in signature security and humancomputer interaction. | Hang Guo Ji Wan Haobin Wang Hanxiang Wu Chen Xu Liming Miao Mengdi Han Haixia Zhang | 2021 | Research2021，，1： | 4 |
| 7 | Effect of Sheng-jiang powder on multiple-organ inflammatory injury in acute pancreatitis in rats fed a high-fat diet显示文摘BACKGROUND Obesity worsens inflammatory organ injury in acute pancreatitis(AP), but there is no effective preventive strategy. Sheng-jiang powder(SJP) has been shown to alleviate multiple-organ inflammatory injury in rats with high-fat diet-induced obesity. Hence, SJP is supposed to have an effect on multiple-organ inflammatory injury in AP in rats fed a high-fat diet.AIM To explore how obesity may contribute to aggravating inflammatory organ injury in AP in rats and observe the effect of SJP on multiple-organ inflammatory injury in AP in rats fed a high-fat diet.METHODS Rats were randomly assigned to a control group(CG), an obese group(OG), and an SJP treatment group(SG), with eight rats per group. The rats in the OG and SG were fed a high-fat diet. From the third week, the rats in the SG were given oral doses of SJP(5 g/kg of body weight). After 12 wk, AP was induced in the three groups. Serum amylase level, body weight, Lee's index, serum biochemistry parameters, and serum inflammatory cytokine and tissue cytokine levels were assessed, and the tissue histopathological scores were evaluated and compared.RESULTS Compared with the CG, serum triglyceride, total cholesterol, interleukin-6, and interleukin-10 levels were significantly higher in the OG, and serum high-density lipoprotein cholesterol level was significantly lower in the OG. Moreover,enhanced oxidative damage was observed in the pancreas, heart, spleen, lung,intestine, liver, and kidney. Evidence of an imbalanced antioxidant defense system, especially in the pancreas, spleen, and intestine, was observed in the obese AP rats. Compared with the OG, serum high-density lipoprotein cholesterol, interleukin-10, and superoxide dismutase expression levels in the pancreas, spleen, and intestine were increased in the SG. Additionally, SJP intervention led to a decrease in the following parameters: body weight; Lee's index; serum triglyceride levels; serum total cholesterol levels; malondialdehyde expression levels in the pancreas, heart, spleen, lung, and liver; myeloperoxidase expression levels in the lung; and pathological scores in the liver.CONCLUSION Obesity may aggravate the inflammatory reaction and pathological multipleorgan injury in AP rats, and SJP may alleviate multiple-organ inflammatory injury in AP in rats fed a high-fat diet. | Yi-Fan Miao Hong-Xin Kang Juan Li Yu-Mei Zhang Hong-Yu Ren Lv Zhu Huan Chen Ling Yuan Hang Su Mei-Hua Wan Wen-Fu Tang | 2019 | World Journal of Gastroenterology2019，25，6： | 4 |
| 8 | Thymosin Alpha-1 Inhibits Complete Freund’s Adjuvant-Induced Pain and Production of Microglia-Mediated Pro-inflammatory Cytokines in Spinal Cord显示文摘Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord. | Yunlong Xu Yanjun Jiang Lin Wang Jiahua Huang Junmao Wen Hang Lv Xiaoli Wu Chaofan Wan Chuanxin Yu Wenjie Zhang Jiaying Zhao Yinqi Zhou Yongjun Chen | 2019 | Neuroscience Bulletin2019，35，4： | 4 |
| 9 | Comparative transcriptome analysis of different heat stress responses between self-root grafting line and heterogeneous grafting line in rose显示文摘Rose is sensitive to high temperature which will make the rose go into a semi-dormancy state.Grafting is an excellent way to enhance rose heat tolerance.Here,heat-tolerant Rosa multiflora‘Huanong Wuci 1'(W)and heat-sensitive Rosa chinensis‘Old Blush’(X)were selected as experimental materials.The RNA-seq technique was used to investigate the transcriptomes of self-root grafting line(XX0),heterogeneous grafting line(XW0),self-root grafting line under 6 h heat stress(XX6),and heterogeneous grafting line under 6 h heat stress(XW6).Under high temperature stress,multiple signaling pathways were activated,moreover,a large number of transcription factors and functional genes were induced,especially the HSFs and HSPs with remarkably upregulated expression levels.The GO analysis showed that the differences in the expressions of the genes related to fatty acids and carbohydrates were observed between self-root grafting line and heterogeneous grafting line.In addition,14 P450s were differentially expressed,and one lectin gene was up-regulated in XW0 vs XW6,but down-regulated in XX0 vs XX6.Considering physiological and biochemical traits such as relative electrolyte leakage,SOD activity,proline,and total soluble sugars,DEGs involved in these processes may be key factors to resist high temperature.The present study provides an insight into the complex mechanism underlying grafting in response to heat stress.Our results indicate that grafting is an effective way to improve rose heat resistance. | Wenquan Qi Chunling Zhang Wenjing Wang Zhe Cao Song Li Hang Li Wan Zhu Yongqi Huang Manzhu Bao Yanhong He Riru Zheng | 2021 | Horticultural Plant Journal2021，7，3： | 3 |
| 10 | Aerosol printing and photonic sintering of bioresorbable zinc nanoparticle ink for transient electronics manufacturing显示文摘Bioresorbable electronics technology can potentially lead to revolutionary applications in healthcare, consumer electronics, and data security. This technology has been demonstrated by various functional devices. However, majority of these devices are realized by CMOS fabrication approaches involving complex and time-consuming processes that are high in cost and low in yield. Printing electronics technology represents a series of printing and post processing techniques that hold promise to make high performance bioresorbable electronics devices. But investigation of printing approaches for bioresorbable electronics is very limited. Here we demonstrate fabrication of conductive bioresorbable patterns using aerosol printing and photonic sintering approaches. Experimental results and simulation reveals that ink compositions, photonic energy, film thickness, and ventilation conditions may influence the effect of photonic sintering. A maximum conductivity of 22321.3 S/m can be achieved using 1 flash with energy of 25.88 J/cm^2 with duration of 2 ms. By combining two cascaded sintering procedures using flash light and laser further improve the conductivity to 34722.2 S/m. The results indicate that aerosol printing and photonic sintering can potentially yield mass fabrication of bioresorbable electronics, leading to prevalence of printable bioresorbable technology in consumer electronics and biomedical devices. | Bikram Kishore MAHAJAN BrANDon LUDWIG Wan SHOU Xiaowei YU Emmanuel FREGENE Hang XU Heng PAN Xian HUANG | 2018 | Science China(Information Sciences)2018，61，6： | 2 |
| 11 | Bioprocess-inspired preparation of silica with varied morphologies and potential in lithium storage显示文摘As one of the most delicate bioprocesses in nature,biosilicification is closely related to biosilica with various morphologies,and has provided abundant inspiration to materials synthesis.In the present study,to explore the biosilica formation process and fabricate silica with an exquisite microstructure for lithiumion battery(LIB)electrodes,a bacterial phage(M13)is used as a biotemplate to synthesize silica with diverse morphologies:cylinders,hexagonal prisms,assemblies of smaller cylinders and nanowires.A facile ethanol bath method is conducted to coat the nanowires with nitrogen-containing carbon and carbon-coated SiO_(2) nanowires with mesochannels(C@msSiO_(2) NWs)are first used as anode materials for LIBs.Attributed to the uniform carbon coating and parallel mesochannel structure,the electronic conductivity and capacity to accommodate volume variations were significantly improved.In the electrochemical perfo rmance test,the composites calcined at 750℃(C@msSiO_(2) NWs-750)show an impressive capacity of 653 mA h g^(-1) at a current density of 500 mA g^(-1) and stability(1000 cycles).In view of the electrochemical test outcomes,the prepa ration of a sophisticated structure with an outstanding potential is easily achieved via a biomimetic strategy. | Fuqiang Wan Wenxuan Wang Zhaoyong Zou Hao Xie Hang Ping Zhengyi Fu | 2021 | Journal of Materials Science & Technology2021，，13： | 2 |
| 12 | Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development显示文摘Pentatricopeptide repeat(PPR) proteins, composing one of the largest protein families in plants,are involved in RNA binding and regulation of organelle RNA metabolism at the posttranscriptional level. Although several PPR proteins have been implicated in endosperm development in rice(Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18(flo18) with pleiotropic defects in both reproductive and vegetative development.Map-based cloning and complementation tests showed that FLO18 encodes a mitochondriontargeted P-type PPR protein with 15 PPR motifs.Mitochondrial function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5′-end processing of mitochondrial nad5 transcripts encoding subunit 5 of nicotinamide adenine dinucleotide hydrogenase. These results suggested that FLO18 is involved in 5′-end processing of nad5 messenger RNA and plays an important role in mitochondrial function and endosperm development. | Mingzhou Yu Mingming Wu Yulong Ren Yihua Wang Jingfang Li Cailin Lei Yinglun Sun Xiuhao Bao Hongming Wu Hang Yang Tian Pan Yongfei Wang Ruonan Jing Mengyuan Yan Houda Zhang Lei Zhao Zhichao Zhao Xin Zhang Xiuping Guo Zhijun Cheng Bing Yang Ling Jiang Jianmin Wan | 2021 | Journal of Integrative Plant Biology2021，63，5： | 2 |
| 13 | Development and validation of a nomogram for predicting overall survival in cirrhotic patients with acute kidney injury显示文摘BACKGROUND Acute kidney injury(AKI)is a common and severe complication in patients with cirrhosis,and is associated with poor prognosis.Therefore,identifying cirrhotic patients with AKI who are at high risk of mortality is very important and may be helpful for providing timely medical interventions to improve the prognosis of these patients.However,studies focused on investigating the risk factors for the mortality of cirrhotic patients with AKI were scarce.AIM To identify risk factors for mortality and establish a nomogram for predicting the prognosis of these patients.METHODS Two hundred fifty consecutive patients with cirrhosis and AKI were recruited and randomly divided into training cohort(n=173)and validation cohort(n=77).In the training cohort,potential risk factors for death were identified by performing a Cox regression analysis,and a nomogram was established.The predictive performance of the nomogram was internally and externally validated by calculating the area under the receiver operating characteristic curve(AUROC),constructing a calibration curve and performing decision curve analysis.RESULTS The serum sodium level,international normalized ratio,peak serum creatinine level>1.5 mg/dL,the presence of hepatic encephalopathy and diabetes were potential risk factors for mortality of cirrhotic patients with AKI in the training dataset.A prognostic nomogram incorporating these variables was established for predicting the overall survival of these patients.Compared with Child-Turcotte-Pugh,the model for end-stage liver disease(MELD)and the MELD-Na scores,the nomogram in predicting 90-and 180-d mortality exhibited better discriminatory power with AUROCs of 0.792 and 0.801 for the training dataset and 0.817 and 0.862 for the validation dataset,respectively.With a nomogram score of 98,patients were divided into low-and high-risk groups,and high-risk patients had a higher mortality rate.CONCLUSION A prognostic nomogram displayed good performance for predicting the overall survival of cirrhotic patients with AKI,and will assist clinicians in evaluating the prognosis of these patients. | Yi-Peng Wan An-Jiang Wang Wang Zhang Hang Zhang Gen-Hua Peng Xuan Zhu | 2022 | World Journal of Gastroenterology2022，28，30： | 2 |
| 14 | Direct detection of a single[4Fe-4S]cluster in a tungsten-containing enzyme:Electrochemical conversion of CO_(2) into formate by formate dehydrogenase显示文摘The conversion of CO_(2) into fuels and valuable chemicals is one of the central topics to combat climate change and meet the growing demand for renewable energy.Herein,we show that the formate dehydrogenase from Clostridium ljungdahlii(ClFDH)adsorbed on electrodes displays clear characteristic voltammetric signals that can be assigned to the reduction and oxidation potential of the[4Fe-4S]^(2+/+)cluster under nonturnover conditions.Upon adding substrates,the signals transform into a specific redox center that engages in catalytic electron transport.ClFDH catalyzes rapid and efficient reversible interconversion between CO_(2) and formate in the presence of substrates.The turnover frequency of electrochemical CO_(2) reduction is determined as 1210 s^(-1) at 25℃ and pH 7.0,which can be further enhanced up to 1786 s^(-1) at 50℃.The Faradaic efficiency at−0.6 V(vs.standard hydrogen electrode)is recorded as 99.3%in a 2-h reaction.Inhibition experiments and theoretical modeling disclose interesting pathways for CO_(2) entry,formate exit,and OCN−competition,suggesting an oxidation-state-dependent binding mechanism of catalysis.Our results provide a different perspective for understanding the catalytic mechanism of FDH and original insights into the design of synthetic catalysts. | Wenjin Li Yanxin Gao Xuan Sun Lei Wan Haishuo Ji Hang Luo Yao Tian Hao Song Geng Wu Liyun Zhang | 2023 | Carbon Energy2023，5，5： | 2 |
| 15 | Fingerprint image enhancement: algo- rithm and performance evaluation显示文摘 | Hang L Wan Yifei Jain A K | 1998 | IEEE Trans on Pattern Ana- lysis and Machine Intelligence1998，20，8： | 1 |
| 16 | Pore Pressure Accumulation of Anisotropically Consolidated Soft Clay Subjected to Complex Loads Under Different Stress Paths显示文摘Owing to different influence factors of foundation soil,the initial stress state of the soil under various working conditions is complex.To simulate this situation,in this paper,a series of tests on undisturbed soft clay under pure principal stress axis rotation were carried out by using the hollow cylinder apparatus(HCA).The influence of initial consolidation angle ζ(the angle between the vertical direction and direction of the applied load in consolidation)and intermediate principal stress coefficient b on pore water pressure accumulation of undisturbed soft clay were mainly studied.The test results show that,during pure principal stress axis rotation,the pore water pressure accumulation of the undisturbed soft clay fluctuates and increases with the rotation of the major principal stress;the values of major principal stress anglesα,corresponding to the peak value of the pore water pressure in a certain cycle,are different with different initial consolidation angles;the pore water pressure accumulation of soft clay is greatly affected by the intermediate principal stress coefficient b.With the fixed initial consolidation angle ζ,the variation trend of the maximum pore water pressure for each cycle is appropriately the same with different b values.With the increase of cycles,the difference value of pore water pressure between b=0 and b=1 in each cycle increases gradually with different initial consolidation angles ζ.While with different initial consolidation anglesζ,the increase of the pore water pressure when b increases from 0 to 0.5 is different with that when b increases from 0.5 to 1;the variation of maximum pore water pressure withζis significantly affected by the value of b;the value of maximum pore water pressure increases with the cycle number increases under all test conditions,but the growth rate decreases gradually.And the variation of maximum pore water pressure with the cycle number N is obviously influenced by both ζ and b. | WANG Yu-ke WAN Yong-shuai RUAN Hang YU Xiang SHAO Jing-gan REN De-bo | 2021 | China Ocean Engineering2021，35，3： | 1 |
| 17 | Structural basis of pre-m RNA splicing显示文摘 | Hang J Wan R Yan C | 2015 | Science2015，349，： | 1 |
| 18 | Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development显示文摘Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR protein.Mutation of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA. | Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli Chen Xiuhao Bao Rongbo Chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，3： | 1 |
| 19 | Structural basis of pre-mRNA splic- ing显示文摘 | Hang J Wan R Yan C | 2015 | Science2015，349，6253： | 1 |
| 20 | Development and validation of a C0-C7 FE complex for biomechanical study显示文摘 | QING HANG ZHANG EE CHON TEO HONG WAN NG | 2005 | Journal of Biomechanical Engineering2005，127，5： | 1 |
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