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| 1 | Field evaluation of effects of transgenic cry1Ab/cry1Ac,cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators显示文摘The impacts of transgenic Bt rice on target pests and their predators need to be clarified prior to the commercialization of Bt rice.In this study,the percentages of folded leaves of three transgenic Bt rice lines and non-transgenic parental rice line caused by Cnaphalocrocis medinalis were studied over two successive growing seasons.In addition,the population densities,relative abundance and population dynamics of C.medinalis and four species of its natural arthropod predators were investigated at three sites in China.The results showed that rice line significantly affected the percentages of folded leaves and population densities of C.medinalis larvae.Significantly higher percentages of folded leaves were observed on the non-transgenic rice compared with the three transgenic Bt rice on most sampling dates.Significantly higher densities of C.medinalis larvae and higher relative abundance of C.medinalis within phytophages were found on non-transgenic rice compared with three transgenic Bt rice at different sites across the study period.The population dynamics of C.medinalis larvae were significantly affected by rice line,rice line×sampling date,rice line×year,rice line×sampling date×year.However,there was little,if any,significant difference in the relative abundance,population density and population dynamics of the four arthropod predators between the three Bt rice lines and non-transgenic rice.The results of this study indicate that the Bt toxin in transgenic Bt rice can effectively suppress the occurrence of C.medinalis,but has no significant effects on the occurrence of the four predatory arthropod species. | XU XueLiang HAN Yu WU Gang CAI WanLun YUAN BenQi WANG Hui LIU FangZhou WANG ManQun HUA HongXia | 2011 | Science China(Life Sciences)2011，54，11： | 14 |
| 2 | Liquid-liquid phase separation in biology: mechanisms,physiological functions and human diseases显示文摘Cells are compartmentalized by numerous membrane-enclosed organelles and membraneless compartments to ensure that a wide variety of cellular activities occur in a spatially and temporally controlled manner. The molecular mechanisms underlying the dynamics of membrane-bound organelles, such as their fusion and fission, vesicle-mediated trafficking and membrane contactmediated inter-organelle interactions, have been extensively characterized. However, the molecular details of the assembly and functions of membraneless compartments remain elusive. Mounting evidence has emerged recently that a large number of membraneless compartments, collectively called biomacromolecular condensates, are assembled via liquid-liquid phase separation(LLPS). Phase-separated condensates participate in various biological activities, including higher-order chromatin organization,gene expression, triage of misfolded or unwanted proteins for autophagic degradation, assembly of signaling clusters and actin-and microtubule-based cytoskeletal networks, asymmetric segregations of cell fate determinants and formation of pre-and post-synaptic density signaling assemblies. Biomacromolecular condensates can transition into different material states such as gel-like structures and solid aggregates. The material properties of condensates are crucial for fulfilment of their distinct functions, such as biochemical reaction centers, signaling hubs and supporting architectures. Cells have evolved multiple mechanisms to ensure that biomacromolecular condensates are assembled and disassembled in a tightly controlled manner. Aberrant phase separation and transition are causatively associated with a variety of human diseases such as neurodegenerative diseases and cancers. This review summarizes recent major progress in elucidating the roles of LLPS in various biological pathways and diseases. | Hong Zhang Xiong Ji Pilong Li Cong Liu Jizhong Lou Zheng Wang Wenyu Wen Yue Xiao Mingjie Zhang Xueliang Zhu | 2020 | Science China(Life Sciences)2020，63，7： | 11 |
| 3 | Land-surface processes and summer-cloud-precipitation characteristics in the Tibetan Plateau and their effects on downstream weather:a review and perspective显示文摘Correct understanding of the land-surface processes and cloud-precipitation processes in the Tibetan Plateau(TP)is an important prerequisite for the study and forecast of the downstream activities of weather systems and one of the key points for understanding the global atmospheric movement.In order to show the achievements that have been made,this paper reviews the progress on the observations for the atmospheric boundary layer,land-surface heat fluxes,cloud-precipitation distributions and vertical structures by using ground-and space-based multiplatform,multisensor instruments and the effect of the cloud system in the TP on the downstream weather.The results show that the form drag related to the topography,land-atmosphere momentum and scalar fluxes is an important part of the parameterization process.The sensible heat flux decreased especially in the central and northern TP caused by the decrease in wind speeds and the differences in the ground-air temperatures.Observations show that the cloud and precipitation over the TP have a strong diurnal variation.Studies also show the compressed-air column in the troposphere by the higher-altitude terrain of the TP makes particles inside clouds vary at a shorter distance in the vertical direction than those in the non-plateau area so that precipitation intensity over the TP is usually small with short duration,and the vertical structure of the convective precipitation over the TP is obviously different from that in other regions.In addition,the influence of the TP on severe weather downstream is preliminarily understood from the mechanism.It is necessary to use model simulations and observation techniques to reveal the difference between cloud precipitation in the TP and non-plateau areas in order to understand the cloud microphysical parameters over the TP and the processes of the land boundary layer affecting cloud,precipitation and weather in the downstream regions. | Yunfei Fu Yaoming Ma Lei Zhong Yuanjian Yang Xueliang Guo Chenghai Wang Xiaofeng Xu Kun Yang Xiangde Xu Liping Liu Guangzhou Fan Yueqing Li Donghai Wang | 2020 | National Science Review2020，7，3： | 10 |
| 4 | Effects of artificially cultivated biological soil crusts on soil nutrients and biological activities in the Loess Plateau显示文摘Biological soil crusts(BSCs) play an important role in the early succession of vegetation restoration in the Loess Plateau, China. To evaluate the effects of artificially cultivated BSCs on the soil surface micro-environment, we obtained natural moss crusts and moss-lichen crusts from the Loess Plateau of Shaanxi province, and subsequently inoculated and cultivated on horizontal and sloping surfaces of loess soil in a greenhouse. The chemical and biological properties of the subsoil under cultivated BSCs were determined after 10 weeks of cultivation. The results indicated that BSCs coverage was more than 65% after 10 weeks of cultivation. Moss crust coverage reached 40% after 5 weeks of cultivation. Compared with the control, soil organic matter and available nitrogen contents in moss crust with the horizontal treatments increased by 100.87% and 48.23%, respectively; increased by 67.56% and 52.17% with the sloping treatments, respectively; they also increased in moss-lichen crust with horizontal and sloping treatments, but there was no significant difference. Available phosphorus in cultivated BSCs was reduced, soil pH was lower and cationic exchange capacity was higher in cultivated BSCs than in the control. Alkaline phosphatase, urease and invertase activities were increased in artificially cultivated BSCs, and alkaline phosphatase activity in all cultivated BSCs was obviously higher than that in the control. Numbers of soil bacteria, fungi and actinomycetes were increased in the formation process of cultivated BSCs. These results indicate that BSCs could be formed rapidly in short-term cultivation and improve the micro-environment of soil surface, which provides a scientific reference for vegetation restoration and ecological reconstruction in the Loess Plateau, China. | YanMin ZHAO QingKe ZHU Ping LI LeiLei ZHAO LuLu WANG XueLiang ZHENG Huan MA | 2014 | Journal of Arid Land2014，6，6： | 7 |
| 5 | NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging显示文摘It is of great significance to study the brain structure and function in deep-tissue for neuroscience research and bio-medical applications because of the urgent demand for precise theranostics.Three-photon fluorescence microscopic(3PFM)bioimaging excited by the light in near-infrared IIb(NIR-IIb,1,500–1,700 nm)spectral region is one of the most promising imaging techniques with the advantages of high spatial resolution,large imaging depth,and reduced scattering.Herein,a type of NIR-IIb light excitable deep-red emissive semiconducting polymer dots(P-dots)with bright 3PF and large three-photon absorption cross-section(σ3)at 1,550 nm was prepared.Then the P-dots were functionalized with polystyrene polymer polystyrene graft ethylene oxide functionalized with carboxyl groups(PS-PEG-COOH)and modified with NH2-poly(ethylene glycol)(PEG)to synthesis photochemically stable and biocompatible P-dots nanoparticles(NPs).Further the P-dots NPs were utilized for in vivo 3PFM bioimaging of cerebral vasculature with and without the brain skull under 1,550 nm femtosecond(fs)laser excitation.In vivo 3PFM bioimaging of the mice cerebral vasculature at various vertical depths was obtained.Moreover,a vivid three-dimensional structure of the mice vascular architecture beneath the skull was reconstructed.At the depth of 350μm beneath the brain skull,3.8μm blood vessels could still be clearly recognized.NIR-IIb excitable P-dots assisted 3PFM bioimaging has great potential in accurate deep tissue bioimaging. | Nuernisha Alifu Abudureheman Zebibula Hequn Zhang Huwei Ni Liang Zhu Wang Xi Yalun Wang Xueliang Zhang Changfeng Wu Jun Qian | 2020 | Nano Research2020，13，10： | 6 |
| 6 | Mapping epistasis and environment × QTX interaction based on four-omics genotypes for the detected QTX loci controlling complex traits in tobacco显示文摘Using newly developed methods and software, association mapping was conducted for chromium content and total sugar in tobacco leaf, based on four-omics datasets. Our objective was to collect data on genotype and phenotype for 60 leaf samples at four developmental stages, from three plant architectural positions and for three cultivars that were grown in two locations. Association mapping was conducted to detect genetic variants at quantitative trait SNP(QTS) loci, quantitative trait transcript(QTT) differences,quantitative trait protein(QTP) variability, and quantitative trait metabolite(QTM) changes,which can be summarized as QTX locus variation. The total heritabilities of the four-omics loci for both traits tested were 23.60% for epistasis and 15.26% for treatment interaction.Epistasis and environment × treatment interaction had important impacts on complex traits at all-omics levels. For decreasing chromium content and increasing total sugar in tobacco leaf, six methylated loci can be directly used for marker-assisted selection, and expression of ten QTTs, seven QTPs and six QTMs can be modified by selection or cultivation. | Liyuan Zhou Ruiyuan Li Longjiang Fan Yuewei Shi Zhihong Wang Shengdong Xie Yijie Gui Xueliang Ren Jun Zhu | 2013 | The Crop Journal2013，1，2： | 4 |
| 7 | Voltammetric Determination of Epinephrine in the Presence of Uric Acid Based on Aminated Graphene and Ag NPs Hybrid Membrane Modified Electrode显示文摘 | XU Huanhuan WANG Xueliang CHEN Rong YU Zhangyu | 2014 | Chemical Research in Chinese Universities2014，30，2： | 3 |
| 8 | P68 RNA helicase is a nucleocytoplasmic shuttling protein显示文摘P68 RNA helicase 是原型的死了的盒子 RNA helicase。蛋白质在早机关开发和成熟起一个很重要的作用。与在 transcriptional 规定并且 pre-mRNA 拼接的蛋白质的功能一致, p68 被发现主要在房间原子核本地化。然而,最近的实验表明蛋白质的短暂细胞质的本地化。我们这里报导那 p68 在原子核和细胞质之间的梭。p68 的 nucleocytoplasmic shuttling 被二个原子本地化信号和二个原子出口信号顺序元素调停。我们的实验揭示那 p68 经由一条古典 RanGTPase 依赖的小径的梭。 | Haizhen Wang Xueliang Gao Yun Huang Jenny Yang Zhi-Ren Liu | 2009 | Cell Research2009，19，12： | 3 |
| 9 | A Strategy for Merging Objective Estimates of Global Daily Precipitation from Gauge Observations, Satellite Estimates, and Numerical Predictions显示文摘This paper describes a strategy for merging daily precipitation information from gauge observations, satellite estimates(SEs), and numerical predictions at the global scale. The strategy is designed to remove systemic bias and random error from each individual daily precipitation source to produce a better gridded global daily precipitation product through three steps.First, a cumulative distribution function matching procedure is performed to remove systemic bias over gauge-located land areas. Then, the overall biases in SEs and model predictions(MPs) over ocean areas are corrected using a rescaled strategy based on monthly precipitation. Third, an optimal interpolation(OI)–based merging scheme(referred as the HL-OI scheme)is used to combine unbiased gauge observations, SEs, and MPs to reduce random error from each source and to produce a gauge—satellite–model merged daily precipitation analysis, called BMEP-d(Beijing Climate Center Merged Estimation of Precipitation with daily resolution), with complete global coverage. The BMEP-d data from a four-year period(2011–14) demonstrate the ability of the merging strategy to provide global daily precipitation of substantially improved quality.Benefiting from the advantages of the HL-OI scheme for quantitative error estimates, the better source data can obtain more weights during the merging processes. The BMEP-d data exhibit higher consistency with satellite and gauge source data at middle and low latitudes, and with model source data at high latitudes. Overall, independent validations against GPCP-1DD(GPCP one-degree daily) show that the consistencies between BMEP-d and GPCP-1DD are higher than those of each source dataset in terms of spatial pattern, temporal variability, probability distribution, and statistical precipitation events. | Suping NIE Tongwen WU Yong LUO Xueliang DENG Xueli SHI Zaizhi WANG Xiangwen LIU Jianbin HUANG | 2016 | Advances in Atmospheric Sciences2016，33，7： | 3 |
| 10 | Uncertainty analysis of cross-calibration for HJ-1 CCD camera显示文摘Radiometric calibration of sensor is the basis of quantitative remote sensing,and uncertainty analysis is critical to ensure the accuracy of cross-calibration.Therefore,firstly,cross-calibration formulas were improved by redefining calibration coefficient and spectral band matching factor.In these formulas,cci was redefined as the calibration coefficient of normalized apparent reflectance,and spectral band matching factor as the ratio of normalized apparent reflectance.Secondly,based on the contrast of ideal and actual conditions in cross-calibration,8 sources of cross-calibration uncertainty were proposed:calibration uncertainty of standard sensor;pixel matching uncertainty;spectral band matching factor uncertainty caused by site altitude setting error,atmospheric parameters setting error,surface spectrum source,surface bidirectional reflectance characteristic,and error of atmospheric radiative transfer model;and uncertainty caused by other factors which were not considered.Finally,the contribution of each uncertainty was further analyzed and discussed for the HJ-1 CCD camera.The results provide many valuable references for evaluating the feasibility of alternative cross-calibration measurements. | WANG Zuo XIAO PengFeng GU XingFa FENG XueZhi LI XiaoYing GAO HaiLiang LI Hui LIN JinTang ZHANG XueLiang | 2013 | Chinese Science Bulletin2013，58，7： | 3 |
| 11 | Pyruvate Kinase M2 Regulates Gene Transcription by Acting as a Protein Kinase显示文摘 | Xueliang Gao Haizhen Wang Jenny J. Yang Xiaowei Liu Zhi-Ren Liu | 2012 | Molecular Cell2012，，5： | 2 |
| 12 | Role of JAK-STAT3 signaling pathway during neuronal differentiation of rat bone marrow mesenchymal stem cells显示文摘BACKGROUND: Recent studies regarding neuronal differentiation of mesenchymal stem cells (MSCs) have primarily focused on induction methods and transplantation in vivo.However,knowledge about the intrinsic regulatory mechanisms underlying neuronal induction of MSCs remains limited and unclear.OBJECTIVE: To elucidate the role of JAK-STAT3 signaling pathway during neuronal differentiationof MSCs using a combination of the JAK-STAT3 signaling inhibitor AG490 and growth factors.DESIGN,TIME AND SETTING: Neural,molecular,biomedical,in vitro experiment was performed at the Laboratory of Pharmacology,School of Pharmacy,Nanjing Medical University between Marchand December 2008 MATERIALS: An inhibitor of the JAK-STAT3 signaling pathway was purchased from Calbiochem,USA.Antibody kit for total and phosphorylated STAT3 was purchased from Cell Signaling,USA.METHODS: MSCs from passage 3 were assigned to non-induced,growth factor,and AG490 groups.MAIN OUTCOME MEASURE: The number of cells expressing neuron-specific enolase,microtubule-associated protein,and glial fibrillary acidic protein were determined by immunocytochemistry.Total and phosphorylated (Tyr705) expression levels of STAT3 protein were measured by Western blot analysis.RESULTS: MSCs were transdifferentiated into neuronaland astrocyte-like phenotypes throughthe induction of epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor.In addition,the JAK-STAT3 signaling pathway was significantly activated during neural differentiation.Expression of phosphorylated (Tyr705) STAT3 was inhibited with AG490 (5 μmol/L) prior to neural induction with epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor;proportion of astrocyte-like cells was significantly reduced (P < 0.01),and the proportion of neuronal-like phenotypes was significantly increased (P < 0.01).CONCLUSION: JAK-STAT3 signaling pathway was shown to regulate neuronal induction of bone marrow MSCs.The proportion of MSC-induced neuronal-like cells was increased following treatment with the JAK-STAT3 signaling inhibitor AG490. | Huaiyong Hao Yongguang Wang Feng Cheng Baosheng Huang Heping Tian Xueliang Dai Lixin Li | 2010 | Neural Regeneration Research2010，5，5： | 2 |
| 13 | Experimental Studies on the Natural Restoration and the Artificial Culture of the Moss Crusts on Fixed Dunes in the Tengger Desert,China显示文摘Soil microbiotic crusts cover extensive portions of the arid and semiarid regions of the world and play an important ecological role.Moss is one of the major components in the crusts.The reproduction and establishment of the mosses are crucial to the formation of moss crusts.Bryum argenteum is the dominant species of moss crusts in the Shapotou region(104°57′E,37°27′N)of the Tengger Desert.In search for the characteristics of natural reproduction and establishment of the mosses,10 quadrates(10×10 cm for each)were obtained by removing the moss crusts in different positions of fixed dunes.These 10 quadrates were observed for 3 years depending on the species’components and coverage.Meanwhile,in the third year,two quadrates(1×1 m for each)were set up in a crustabsent area and two different experiments of the asexual reproduction(broadcast planting and offshoots)were conducted,respectively.The reproductive process was observed under the microscope,and the morphological indicators of the new individuals were measured.The results were compared with the ones from indoor experiments using the same methods.All the results showed the following:(1)70%of the quadrates(i.e.,7 of the 10 quadrates)were recovered within 3–4 years;thus,the quick recovery might be due to the dispersal and reproduction of the fragments of stems and leaves of B.argenteum;(2)as for the two quadrates in the artificial reproduction test,the new plants occupied the uncovered space of the quadrates in 1 month,and there were two main reproduction approaches,one of which was that the stems continually branched and produced young plants,and the other was that the young plants and the fragments of the stems and leaves repeatedly and extensively reproduced protonema,which finally developed into a large number of new plants;(3)the reproductive characteristics were identical,though the protonema in the field was more robust and had more branches than the ones indoors. | Tian Guiquan Bai Xueliang Xu Jie Wang Xiandao | 2006 | Frontiers in Biology2006，1，1： | 2 |
| 14 | Comparative Analyses of Vertical Structure of Deep Convective Clouds Retrieved from Satellites and Ground-Based Radars at Naqu over the Tibetan Plateau显示文摘In order to improve understanding of deep convective clouds over the Tibetan Plateau, characteristics of vertical structure of a deep strong convective cloud over Naqu station and a deep weak convective cloud approximately 100 km to the west of Naqu station, which occurred over 1300-1600 Beijing Time (BT) 9 July 2014 during the Third Tibetan Plateau Atmospheric Science Experiment (TIPEX-Ⅲ), are analyzed, based on multi-source satellite data from TRMM, CloudSat, and Aqua, and radar data from ground-based vertically pointing radars (C-band frequency-modulated continuous-wave radar and KA-band millimeter wave cloud radar). The results are as follows.(1) The horizontal scales of both the deep strong and deep weak convective clouds were small (10-20 km), and their tops were high[15-16 km above sea level (ASL)]. Across the level of 0℃ isotherm in the deep strong convective cloud, the reflectivity increased rapidly, suggesting that the melting process of solid precipitation particles through the 0℃ level played an important role. A bright band located at 5.5 km ASL (i.e., 1 km above ground level) appeared during the period of convection weakening.(2) The reflectivity values from TRMM precipitation radar below 11 km were found to be overestimated compared to those derived from the C-band frequency-modulated continuous-wave radar.(3) Deep convective clouds were mainly ice clouds, and there were rich small ice particles above 10 km, while few large ice particles were found below 10 km. The microphysical processes of deep strong and deep weak convective clouds mainly included mixed-phase process and glaciated process, and the mixed-phase process can be divided into two types:one was the rimming process below the level of -25℃(deep strong convective cloud) or -29℃(deep weak convective cloud) and the other was aggregation and deposition process above that level. The latter process was accompanied with fast increase in ice particle effective radius. The above evidence from space-based and ground-based observational data further clarify the characteristics of vertical structure of deep convective clouds over the Tibetan Plateau, and provide a basis for the evaluation of simulation results of deep convective clouds by cloud models. | Hui Wang Xueliang Guo | 2019 | Journal of Meteorological Research2019，33，3： | 2 |
| 15 | Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat显示文摘Motile cilia and flagella are microtubule-based organelles important for cell locomotion and extracellular liquid flow through beating. Although axonenal dyneins that drive ciliary beat have been extensively studied in unicellular Chlamydomonas, to what extent such knowledge can be applied to vertebrate is poorly known. In Chlamydomonas, Dynein-f controls flagellar waveforms but is dispensable for beating. The flagellar assembly of its heavy chains (HCs) requires its intermediate chain (IC) IC140 but not IC138. Here we show that, unlike its Chlamydomonas counte『part, vertebrate Dynein-f is essential for ciliary beat. We confirmed that Wdr78 is the vertebrate orthologue of IC3 Wdr78 associated with Dynein-f subunits such as Dnah2 (a HC) and Wdr63 (IC140 orthologue). It was expressed as a motile cilium-specific protein in mammalian cells. Depletion of Wdr78 or Dnah2 by RNAi paralyzed mouse ependymal cilia. Zebrafish Wdr78 morphants displayed ciliopathy-related phenotypes, such as curved bodies, hydrocephalus, abnormal otolith, randomized left-right asymmetry, and pronephric cysts, accompanied with paralyzed pronephric cilia. Furthermore, all the HCs and ICs of Dynein-f failed to localize in the Wdr78-depleted mouse ependymal cilia. Therefore, both the functions and subunit dependency of Dynein-fare altered in evolution, probably to comply with ciliary roles in higher organisms. | Yirong Zhang Yawen Chen Jianqun Zheng Juan Wang Shichao Duan Wei Zhang Xiumin Yan Xueliang Zhu | 2019 | Journal of Molecular Cell Biology2019，11，5： | 2 |
| 16 | Tangential damping and its dissipation factor models of joint interfaces based on fractal theory with simulations显示文摘 | ZHANG Xueliang WANG Nanshan LAN Guosheng | 2014 | ASME of Journal of Tribology2014，136，1： | 1 |
| 17 | Dehydration of ethyl acetate–water mixtures using PVA/ceramic composite pervaporation membrane显示文摘 | Shanshan Xia Xueliang Dong Yuexin Zhu Wang Wei Fenjuan Xiangli Wanqin Jin | 2010 | Separation and Purification Technology2010，，1： | 1 |
| 18 | Effects of calcium carbonate and compounding sequence on the phase morphology and properties of maleic anhydride-grafted polypropylene compatibilized polyamide 6/ polypropylene blend 显示文摘 | Wang Li Ling Xueliang Shi Chen | 2012 | Journal of Applied Polymer Science2012，，： | 1 |
| 19 | An electrochemical DNA biosensor for highly sensitive detection of phosphinothricin acetyltransferase gene sequence based on polyaniline-(mesoporous nanozirconia)/poly-tyrosine film显示文摘 | YANG Jie WANG Xueliang SHI Huaqiang | 2012 | Sensors and Actuators B:Chemical2012，162，1： | 1 |
| 20 | Facile controlled synthesis and growth mechanisms of flower-like and tubular MnO 2 nanostructures by microwave-assisted hydrothermal method显示文摘 | Yongliang Li Jiajun Wang Yong Zhang Mohammad Norouzi Banis Jian Liu Dongsheng Geng Ruying Li Xueliang Sun | 2011 | Journal of Colloid And Interface Science2011，，1： | 1 |
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