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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Effects of polarization calibration on aerosol optical depth retrieval: An ocean case sensitivity analysis显示文摘Reflectance measurements of both the visible and infrared bands of passive remote sensing sensors are widely used to retrieve aerosol optical depth(AOD) information. This is performed commonly for data obtained over both ocean and land, and these measurements allow for the off line development of a lookup table using radiative transfer models. Owing to molecular and aerosol effects, the reflected light received by the sensor is usually highly polarized. The linear polarization effect may be up to 100%, and the polarization factor of a sensor optical system will change the total intensity as well as the polarization status of the signal reaching the detector. The detector response will be different when the incident light polarization status changes, even if the total intensity remains constant. However, if the polarization calibration is neglected, it will cause obvious errors in the aerosol data retrieval. This is especially true for aerosol optical depth retrieval over an ocean. This measurement relies directly on the reflectance output of the sensor. Cases involving land surfaces are not discussed herein because the inhomogeneous properties conceal the error due to polarization. Taking the 550 and 860 nm bands as examples, the difference between the real top-of-atmosphere(TOA) reflectance and the reflectance reaching the detector is calculated using three different sensor polarization standards according to the Sea-viewing Wide Field-of-view Sensor(Sea Wi FS) and Moderate Resolution Imaging Spectroradiometer(MODIS) standards. The differences in AOD retrieval are also demonstrated using the lookup table developed previously from a vector radiative transfer code. The results reveal that under a normal situation in which the AOD is 0.15, the maximum AOD retrieval error could reach 0.04 in 550 nm but only 0.02 in 860 nm for the dust aerosol model. For the soot aerosol model, the maximum AOD retrieval error is 0.1 in 550 nm and 0.12 in 860 nm, indicating that the lack of polarization calibration will lead to large errors in aerosol retrieval over an ocean. | LIU TianYue CHI TianHe CHEN Wei | 2015 | Science China Earth Sciences2015，58，6： | 1 |
| 3 | Performance Evaluation and Optimization of a Novel System Combining a Photovoltaic/Thermal Subsystem&an Organic Rankine Cycle Driven by Solar Parabolic Trough Collector显示文摘A novel coupling system that combines a photovoltaic/thermal(PV/T)subsystem and an Organic Rankine Cycle(ORC)driven by solar parabolic trough collector(PTC)is presented in this paper.The mathematical model is initially built.On the basis,the influence of area ratio of two collectors(PV/T and PTC)on the performance of system is discussed.The results show that the optimal area ratio of PV/T to PTC is 8:2,which can achieve the maximum energy output.Moreover,the performance of the coupling system and two independent systems(PV/T and ORC system)are compared and analyzed.The results show that the coupling system is more reliable and its total output energy(heat and electricity)is the highest,compared with the other two independent systems.The solar energy utilization efficiency of the coupling system is 40%higher than that of the other two independent systems in the steady-state simulation.Moreover,the annual output energy per unit area collector of the coupling system is 13%higher than that of the other two independent systems in the dynamic simulation.Furthermore,in the dynamic simulation of a typical day,the PV panels’temperature of the coupling system is 5℃–7℃ lower than that of the independent PV/T system.It means that the power generation efficiency of PV panels can be increased by 1.5%–3.5%.This study aims to explore the operation characteristics of the novel solar energy utilization coupling system and promote the development of renewable energy utilization models,which provides a reference for the design and optimization of related energy systems. | LIU Zhijian WEI Tianhe WU Di ZHANG Yulong LI Guiqiang YANG Xinyan | 2021 | Journal of Thermal Science2021，30，5： | 1 |
| 4 | Screening the effective components in treating dampness stagnancy due to spleen deficiency syndrome and elucidating the potential mechanism of Poria water extract显示文摘Poria is an important medicine for inducing diuresis to drain dampness from the middle energizer.However,the specific effective components and the potential mechanism of Poria remain largely unknown.To identify the effective components and the mechanism of Poria water extract(PWE)to treat dampness stagnancy due to spleen deficiency syndrome(DSSD),a rat model of DSSD was established through weight-loaded forced swimming,intragastric ice-water stimulation,humid living environment,and alternate-day fasting for 21 days.After 14 days of treatment with PWE,the results indicated that PWE increased fecal moisture percentage,urine output,D-xylose level and weight;amylase,albumin,and total protein levels;and the swimming time of rats with DSSD to different extents.Eleven highly related components were screened out using the spectrum-effect relationship and LC-MS.Mechanistic studies revealed that PWE significantly increased the expression of serum motilin(MTL),gastrin(GAS),ADCY5/6,p-PKAα/β/γcat,and phosphorylated cAMP-response element binding protein in the stomach,and AQP3 expression in the colon.Moreover,it decreased the levels of serum ADH,the expression of AQP3 and AQP4 in the stomach,AQP1 and AQP3 in the duodenum,and AQP4 in the colon.PWE induced diuresis to drain dampness in rats with DSSD.Eleven main effective components were identified in PWE.They exerted therapeutic effect by regulating the AC-cAMP-AQP signaling pathway in the stomach,MTL and GAS levels in the serum,AQP1 and AQP3 expression in the duodenum,and AQP3 and AQP4 expression in the colon. | LI Huijun ZHANG Dandan WANG Tianhe LUO Xinyao XIA Heyuan PAN Xiang HAN Sijie YOU Pengtao WEI Qiong LIU Dan ZOU Zhongmei YE Xiaochuan | 2023 | Chinese Journal of Natural Medicines2023，21，2： | 0 |
| 5 | AAZ2 induces mitochondrial-dependent apoptosis by targeting PDK1 in gastric cancer显示文摘Drastic surges in intracellular reactive oxygen species(ROS)induce cell apoptosis,while most chemotherapy drugs lead to the accumulation of ROS.Here,we constructed an organic compound,arsenical N-(4-(1,3,2-dithiarsinan-2-yl)phenyl)acrylamide(AAZ2),which could prompt the ROS to trigger mitochondrial-dependent apoptosis in gastric cancer(GC).Mechanistically,by targeting pyruvate dehydrogenase kinase 1(PDK1),AAZ2 caused metabolism alteration and the imbalance of redox homeostasis,followed by the inhibition of phosphoinositide-3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)pathway and leading to the activation of B-cell lymphoma 2(Bcl2)/Bcl2-associated X(Bax)/caspase-9(Cas9)/Cas3 cascades.Importantly,our in vivo data demonstrated that AAZ2 could inhibit the growth of GC xenograft.Overall,our data suggested that AAZ2 could contribute to metabolic abnormalities,leading to mitochondrial-dependent apoptosis by targeting PDK1 in GC. | Yi LI Wenyan SHE Xiaoran XU Yixin LIU Xinyu WANG Sheng TIAN Shiyi LI Miao WANG Chaochao YU Pan LIU Tianhe HUANG Yongchang WEI | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，3： | 0 |
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