|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Reconstruction of precipitation variation from tree rings in recent 1000 years in Delingha, Qinghai显示文摘Using seven well-replicated Qilian juniper (Sabina przewalskii Kom.) ring-width chronologies developed at Zongwulong and Shalike Mts. in the northeastern part of the Qaidam Basin annual precipitation from previous July to current June in the recent 1000 years was re- constructed for Delingha. The reconstruction can capture 63.1% of precipitation variance and the equation was stable over time. For the reconstructed precipitation, wet periods occurred in AD1520—1633 and 1933—2001, whereas dry intervals in 1429—1519 and 1634—1741. In ad- dition, the magnitude in precipitation variation was lower before 1430 with about 15 mm, but it increased to 30 mm during the period of 1430 to 1850. After 1850, the precipitation variance de- creased again. In contrast to the increase in temperature, a decrease in annual precipitation was evident since the 1990s. The agreement in low-frequency variation between the reconstruction and the glacier accumulation and particulate content in Dunde ice cores during the recent several hundred years suggested that the precipitation reconstructed in this study was rather reliable, and represented a regional signal. This 1000-year reconstruction could benefit our understanding of climatic variation in decadal to century-scale in this region, and provide basic data to climate models and to prediction of future climate in the 21st century. | SHAO Xuemei1, HUANG Lei1, LIU Hongbin2, LIANG Eryuan1, FANG Xiuqi3 & WANG Lili1 1. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China 2. National Climate Center, Beijing 100081, China 3. Beijing Normal University, Beijing 100875, China | 2005 | Science China Earth Sciences2005，48，7： | 113 |
| 2 | Recent glacial retreat in High Asia in China and its impact on water resource in Northwest China显示文摘Under the impact of climatic warming, the glaciers in the High Asia in China have been retreating continuously with negative glacial mass balance in recent several decades. The retreat became more intensive in the past 10 years. The spatial pattern of the glacial retreat in the High Asia in China is that the smallest magnitude of retreat is in the inland of the Tibetan Plateau, the magnitude increases from the inland to the margin of the Tibetan Plateau, and the largest magnitude at the margin of the Tibetan Plateau. The glacial retreat in the High Asia in China has an important impact on the water resource of the arid regions in Northwest China. This study shows that the glacial retreat in the 1990s has caused an increase of 5.5% in river runoff in Northwest China. In the Tarim River basin, the increase of river runoff is higher than 5.5%. | YAO Tandong 1,2 ,WANG Youqing 2 ,LIU Shiying 2,1 ,PU Jianchen 2,1 ,SHEN Yongping 2 & LU Anxin 2,1 1.Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100029,China 2.Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou 730000,China | 2004 | Science China Earth Sciences2004，47，12： | 117 |
| 3 | Design of a new dynamical core for global atmospheric models based on some efficient numerical methods显示文摘A careful study on the integral properties of the primitive hydrostatic balance equations for baroclinic atmosphere is carried out, and a new scheme todesign the global adiabatic model of atmospheric dynamics ispresented. This scheme includes a method of weighted equal-areamesh and a fully discrete finite difference method with quadraticand linear conservations for solving the primitive equationsystem. Using this scheme, we established a new dynamical corewith adjustable high resolution acceptable to the availablecomputer capability, which can be very stable without anyfiltering and smoothing. Especially, some important integralproperties are kept unchanged, such as the anti-symmetries of thehorizontal advection operators and the vertical convectionoperator, the mass conservation, the effective energy conservationunder the standard stratification approximation, and so on. Somenumerical tests on the new dynamical core, respectively regardingits global conservations and its integrated performances inclimatic modeling, incorporated with the physical packagesfrom the Community Atmospheric Model Version 2 (CAM2) of NationalCenter for Atmospheric Research (NCAR), are included. | WANG Bin, WAN Hui, Jl Zhongzhen, ZHANG Xin, YU Rucong, YU Yongqiang & LIU HongtaoState Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China Department of Computational Mathematics, Academy of Mathematics and System Sciences, Peking University, Beijing 100871, China | 2004 | Science China Mathematics2004，47，z1： | 92 |
| 4 | Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection显示文摘AIM To identify the property of dendritic cella (DCs) of peripheral blood monocytes (PBMC) in patlents with chronic HBV infection.METHODS Twenty patients with persistent HBV infectlon were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GMCSF, IL-4, FLt-3, TNF-α and 100 mL@ L-1 of fetal calf serum for a total of 10 - 12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation,mytomycin C-treated DC were incubated with allogenic T cells.RESULTS A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLADR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0. 84 for CD86, P< 0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCa simultaneously increased compared with healthy subjects (86± 15 vs 170±22 μmoI@L 1, P<0.05).CONCLUSION The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV. | Fu-Sheng Wang Li-He Xing Ming-Xu Liu Chuan-Lin Zhu Hui-Gang Liu Hui-Fen Wang Zhou-Yun Lei Division of Biological Engineering,~2 Fourth Department of Liver Diseases,Beijing Institute of Infectious Diseases,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2001 | World Journal of Gastroenterology2001，7，4： | 131 |
| 5 | Role of mantle-derived magma in genesis of early Yanshanian granites in the Nanling Range, South China: in situ zircon Hf-O isotopic constraints显示文摘Although a number of petrographic observations and isotopic data suggest that magma mixing is common in genesis of many granite plutons, it is still controversial whether the mantle-derived magmas were involved in granites. We carried out in this study a systematic analysis of in situ zircon Hf-O isotopes for three early Yanshanian intrusions dated at ca. 160 Ma from the Nanling Range of Southeast China. The Qinghu monzonite has very homogeneous zircon Hf-O isotopic compositions, εHf(t) =11.6±0.3 and δ18O=5.4‰±0.3‰. In combination with whole-rock geochemical and Sr-Nd isotopic data, the parental magma of the Qinghu monzonite were likely derived from the partial melting of recently-metasomatized, phlogopite-bearing lithospheric mantle without appreciable crustal contamination. The Lisong and Fogang granites and the mafic microgranular enclaves (MME) within the Lisong granites have a wide range of zircon Hf-O isotopic compositions, with Hf and O isotopes being negatively correlated within each pluton. The Lisong MMEs were crystallized from a mantle-derived magma, similar to the parental magma of the Qinghu monzonite, with small amount of crustal assimilation. The Lisong and Fogang granites were formed by reworking of meta-sedimentary materials by mantle-derived magmas and mixing of the mantle-and sediment-derived melts to varying degrees. It is thus concluded that these two Yanshanian granites in the Nanling Range were formed associated with growth and differentiation of continental crust. | LI XianHua LI WuXian WANG XuanCe LI QiuLi LIU Yu TANG GuoQiang | 2009 | Science China Earth Sciences2009，52，9： | 101 |
| 6 | Spatio-temporal analysis of land-use conversion in the eastern coastal China during 1996-2005显示文摘Based on the acquaintance of the regional background of urban-rural transformational development and investigations on the spot,this paper discusses the holistic situation, dominant factors and mechanism of arable land loss and land for construction occupation in the coastal area of China over the last decade,with the aid of GIS technology.Conclusions of the research are summarized as follows:(1)the arable land had been continuously decreasing from 1996 to 2005,with a loss of 1,708,700 hm 2 and an average decrement of 170,900 hm 2 per year;(2)land for construction increased 1,373,700 hm 2 ,with an average increment of 153,200 hm 2 per year;(3)total area of encroachment on arable land for construction between 1996 and 2005 was 1,053,100 hm 2 ,accounting for 34.03%of the arable land loss in the same period,the percentages of which used for industrial land(INL),transportation land(TRL),rural construction land(RUL)and town construction land(TOL)are 45.03%,15.8%,15.47%and 11.5%,respectively;and(4)the fluctuation of the increase of construction land and encroachment on arable land in the area were deeply influenced by the nation's macroscopic land-use policies and development level of regional economy.The growth of population and advancement of technology promoted the rapid industrialization, construction of transportation infrastructures,rural urbanization and expansion of rural settlements in the eastern coastal area,and therefore were the primary driving forces of land-use conversion. | LIU Yansui WANG Lijuan LONG Hualou | 2008 | Journal of Geographical Sciences2008，18，3： | 93 |
| 7 | Inhibition of metastasis-associated lung adenocarcinoma transcript 1 in CaSki human cervical cancer cells suppresses cell proliferation and invasion显示文摘(MALAT1 ) 联系转移的肺腺癌抄本 1 被建议是一长(7 kb ) 非编码的 RNA。MALAT1 是在许多人的癌的 overexpressed,但是它的功能仍然保持未知。在人的颈的癌症前进调查 MALAT1 的角色,我们设计了并且使用了短发卡 RNA 在 CaSki 房间禁止 MALAT1 表示并且在房间增长和侵略上验证了它的效果。在基因表示的变化被反向的 transcriptasepolymerase 链反应分析。我们的数据证明 MALAT1 通过基因表示的规定涉及颈的癌症房间生长,房间周期前进,和侵略,例如 caspase-3, -8, Bax, Bcl-2,和 Bcl-xL,建议 MALAT1 能有在颈的癌症生物学的重要含意。我们的调查结果在颈的癌症前进说明 MALAT1 的生物意义并且提供 MALAT1 可以在人的颈的癌症的预防作为一个治疗学的目标服务的新奇证据。 | Fengjie Guo Yalin Li Yan Liu Jiajia Wang Yuehui Li Guancheng Li | 2010 | Acta Biochimica et Biophysica Sinica2010，42，3： | 89 |
| 8 | Exogenous plant MIR168a specifically targets mammalian LDLRAPI: evidence of cross-kingdom regulation by microRNA显示文摘 | Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang | 2012 | Cell Research2012，22，1： | 155 |
| 9 | Outline of the Report on Cardiovascular Disease in China,2010显示文摘Major and profound changes have taken place in China over the past 30 years.Rapid socioeconomic progress has exerted a great impact on lifestyle,ranging from food,clothing,working and living conditions,and means of transportation to leisure activities and entertainment.At the same time,new health problems have emerged,and health services are facing new challenges.Presently,cardiovascular diseases (CVD) are among the top health problems of the Chinese people,and pose a serious challenge to all engaged in the prevention and control of these diseases.An epidemic of CVD in China is emerging as a result of lifestyle changes,urbanization and longevity.Both national policy decision-making and medical practice urgently need an authoritative report which comprehensively reflects the trends in the epidemic of CVD and current preventive measures.Since 2005,guided by the Bureau of Disease Prevention of the Ministry of Health of the People's Republic of China and the National Center for Cardiovascular Diseases of China,nationwide experts in the fields of epidemiology,clinical medicine and health economics in the realms of CVD,cerebrovascular disease,diabetes and chronic kidney disease,completed the Report on Cardiovascular Diseases in China every year.The report aims to provide a timely review of the trend of the epidemic and to assess the progress of prevention and control of CVD.In addition,as the report is authoritative,representative and readable,it will become an information platform in the CVD field and an important reference book for government,academic institutes,medical organizations and clinical physicians.This publication is expected to play a positive role in the prevention and control of CVD in China.We present an abstract from the Report on Cardiovascular Diseases in China (2010),including trends in CVD,morbidity and mortality of major CVDs,up-to-date assessment of risk factors,as well as health resources for CVD,and a profile of medical expenditure,with the aim of providing evidence for decision-making in CVD prevention and control programs in China,and of delivering the most authoritative information on CVD prevention and control for all citizens. | HUSheng Shou KONG Ling Zhi GAO Run Lin ZHU Man Lu WANG Wen WANG Yong Jun WU Zhao Su CHEN Wei Wei LIU Ming Bo For the editorial board | 2012 | Biomedical and Environmental Sciences2012，25，3： | 93 |
| 10 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 11 | Tectonics of South China Continent and its implications显示文摘This paper aims at exploring the tectonic characteristics of the South China Continent(SCC)and extracting the universal tectonic rules from these characteristics,to help enrich the plate tectonic theory and better understand the continental dynamic system.For this purpose,here we conduct a multi-disciplinary investigation and combine it with the previous studies to reassess the tectonics and evolution of SCC and propose that the tectonic framework of the continent comprises two blocks,three types of tectonic units,four deformation systems,and four evolutionary stages with distinctive mechanism and tectonic characteristics since the Neoproterozoic.The four evolutionary stages are:(1)The amalgamation and break-up of the Neoproterozoic plates,typically the intracontinental rifting.(2)The early Paleozoic and Mesozoic intracontinental orogeny confined by plate tectonics,forming two composite tectonic domains.(3)The parallel operation of the Yangtze cratonization and intracontinental orogeny,and multi-phase reactivation of the Yangtze craton.(4)The association and differentiation evolution of plate tectonics and intracontinental tectonics,and the dynamic characteristics under the Meso-Cenozoic modern global plate tectonic regime. | ZHANG GuoWei GUO AnLin WANG YueJun LI SanZhong DONG YunPeng LIU ShaoFeng HE DengFa CHENG ShunYou LU RuKui YAO AnPing | 2013 | Science China Earth Sciences2013，56，11： | 88 |
| 12 | A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites: Application in the Study of Rice Metabolomics显示文摘液体 chromatographymass spectrometry (最小公倍数) 基于 metabolomics 被 MS 2 的构造便于光谱从全部的扫描 ESI MS/MS 数据的标签(MS2T ) 图书馆,和广泛地指向的 metabolomics methodusing MS/MS 数据的发展从真标准聚在一起。在这份报告,称为逐步的多重离子 monitoring-enhancedproduct 离子(逐步的 MIMEPI ) 的新奇策略被开发构造 MS2T 图书馆,逐步的 MIM 在被用作调查 scansto 触发 EPI 的获得。698 的一个全部的数字(几乎) 有 MS 2 系列的非冗余的代谢物被获得,哪个 135 代谢物被识别 / 注解。集成数据从我们的 MS2T libraryand 收集了监视的另外的可得到的多重反应(MRM ) 信息,一个广泛地指向的 metabolomics 方法被开发 toquantify 277 代谢物,包括一些植物激素。在米饭叶的脱水回答和自然变化 ofthese 代谢物的评估不仅与代谢物 suchas 血清素衍生物建议了 abscisic 酸(骆驼毛的织物)的协调规定, polyamine 在干旱应力下面结合,而且为 indica 和装饰用的梨树米饭亚种的辨别作为潜在的标记揭示了一些 C-glycosylated 黄酮。新 MS2T 图书馆构造和广泛地指向的 metabolomics 策略能被用作工具 forrice 功能的 genomics.Key 词: | Wei Chen Liang Gong Zilong GUO Wensheng Wang Hongyan Zhang Xianqing Liu SibinYu Lizhong Xiong Jie Luo | 2013 | Molecular Plant2013，6，6： | 162 |
| 13 | Modeling study of regional severe hazes over mid-eastern China in January 2013 and its implications on pollution prevention and control显示文摘The Nested Air Quality Prediction Model System(NAQPMS)was used to investigate the temporal and spatial variations of PM2.5over tropospheric central eastern China in January 2013.The impact of regional transport and its implications on pollution prevention and control were also examined.Comparison between simulated and observed PM2.5showed NAQPMS was able to reproduce the evolution of PM2.5during heavy haze episodes.The results indicated that regional transport of PM2.5played an important role in regional haze episodes in the city cluster including Hebei,Beijing and Tianjin(HBT).The cross-city clusters transport outside HBT and transport among cities inside HBT contributed 20%–35%and 26%–35%of PM2.5as compared with local emission,in HBT respectively.To meet the Air Quality Standards for Grade II,90%,90%and65%of emissions would have to be cut down in Hebei,Tianjin and Beijing,if non-control strategy was taken in the surrounding city clusters of HBT.This implicated that control of emissions in one city cluster is not sufficient to reduce regional haze events,and joint efforts among city clusters are essential.Besides regional transports,two-way feedback between boundary-layer evolution and PM2.5also significantly contributed to the formation of heavy hazes,which contributed 30%of monthly average PM2.5concentration in HBT. | WANG ZiFa LI Jie WANG Zhe YANG WenYi TANG Xiao GE BaoZhu YAN PinZhong ZHU LiLi CHEN XueShun CHEN HuanSheng WAND Wei LI JianJun LIU Bing WANG XiaoYan WAND Wei ZHAO YiLin LU Ning SU DeBin | 2014 | Science China Earth Sciences2014，57，1： | 106 |
| 14 | Mechanism for the formation of the January 2013 heavy haze pollution episode over central and eastern China显示文摘In January 2013,a long-lasting episode of severe haze occurred in central and eastern China,and it attracted attention from all sectors of society.The process and evolution of haze pollution episodes were observed by the'Forming Mechanism and Control Strategies of Haze in China'group using an intensive aerosol and trace gases campaign that simultaneously obtained data at 11 ground-based observing sites in the CARE-China network.The characteristics and formation mechanism of haze pollution episodes were discussed.Five haze pollution episodes were identified in the Beijing-Tianjin-Hebei(Jing-Jin-Ji)area;the two most severe episodes occurred during 9–15 January and 25–31 January.During these two haze pollution episodes,the maximum hourly PM2.5mass concentrations in Beijing were 680 and 530μg m 3,respectively.The process and evolution of haze pollution episodes in other major cities in the Jing-Jin-Ji area,such as Shijiazhuang and Tianjin were almost the same as those observed in Beijing.The external cause of the severe haze episodes was the unusual atmospheric circulation,the depression of strong cold air activities and the very unfavorable dispersion due to geographical and meteorological conditions.However,the internal cause was the quick secondary transformation of primary gaseous pollutants to secondary aerosols,which contributed to the'explosive growth'and'sustained growth'of PM2.5.Particularly,the abnormally high amount of nitric oxide(NOx)in the haze episodes,produced by fossil fuel combustion and vehicle emissions,played a direct or indirect role in the quick secondary transformation of coal-burning sulphur dioxide(SO2)to sulphate aerosols.Furthermore,gaseous pollutants were transformed into secondary aerosols through heterogeneous reactions on the surface of fine particles,which can change the particle’s size and chemical composition.Consequently,the proportion of secondary inorganic ions,such as sulphate and nitrate,gradually increased,which enhances particle hygroscopicity and thereby accelerating formation of the haze pollution. | WANG YueSi YAO Li WANG LiLi LIU ZiRui JI DongSheng TANG GuiQian ZHANG JunKe SUN Yang HU Bo XIN JinYuan | 2014 | Science China Earth Sciences2014，57，1： | 189 |
| 15 | Antitumor activities of human autologous cytokineinduced killer(CIK)cells against hepatocellular carcinoma cells in vitro and in vivo显示文摘AIM: To characterize the anticancer function of cytokine-induced killer cells (CIK) and develop an adoptiveimmunotherapy for the patients with primary hepatocellularcarcinoma (HCC), we evaluated the proliferation rate,phenotype and the antitumor activity of human CIK cellsfrom healthy donors and HCC patients in vitro and in vivo.METHODS: Peripheral blood mononuclear cells (PBMC) fronhealthy donors and patients with primary HCC were incubatedin vitro and induced into ClK cells in the presence of variouscytokines such as interferon-gamma (IFN-γ), interleukin-1(IL-1), IL-2, and monoclonal antibody (mAb) against CD3.The phenotype and characterization of CIK cells wereidentified by flow cytometric analysis. The cytotoxicity of CIKcells was determined by 51 Cr release assay.RESULTS: The CIK cells were shown to be a heterogeneouspopulation with different cellular phenotypes. Thepercentage of CD3+/CD56+ positive cells, the dominanteffector cells, in total CIK cells from healthy donors andHCC patients, significantly increased from 0.1-0.13 % at day0 to 19.0-20.5 % at day 21 incubation, which suggested thatthe CD3+ CD56+ positive cells proliferated faster than othercell populations of CIK cells in the protocol used in thisstudy. After 28 day in vitro incubation, the ClK cells frompatients with HCC and healthy donors increased by morethan 300-fold and 500-fold in proliferation cell number,respectively. CIK cells originated from HCC patientspossessed a higher in vitro antitumor cytotoxic activity onautologous HCC cells than the autologous lymphokine-activated killer (LAK) cells and PBMC cells. In in vivoanimal experiment, CIK cells had stronger effects on theinhibition of tumor growth in Balb/c nude mice bearing BEL-7402-producing tumor than LAK cells (mean inhibitory rate,84.7 % vs 52.8 %, P < 0.05) or PBMC (mean inhibitoryrate, 84.7% vs37.1%, P<0.01).CONCLUSION: Autologous CIK cells are of highly efficientcytotoxic effector cells against primary hepatocellularcarcinoma cells and might serve as an alternative adoptivetherapeutic strategy for HCC patients. | Fu-Sheng Wang Ming-Xu Liu Bing Zhang Ming Shi Zhou-Yun Lei Wen-Bing Sun Qing-You Du Ju-Mei Chen,Division of Biological Engineering,Beijing Institute of Infectious Diseases,Beijing 100039,China Wen-Bing Sun,Department of Surgery,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2002 | World Journal of Gastroenterology2002，8，3： | 108 |
| 16 | Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase显示文摘包含 methyltransferase 建筑群的象 3 一样的 methyltransferase (METTL3 ) 催化 N6-methyladenosine (m6A ) 形成,一个新奇 epitranscriptomic 标记;然而,这建筑群的性质仍然保持大部分未知。这里,我们报导人的 m6A methyltransferase 建筑群, Wilm 的肿瘤 1 伙伴蛋白质(WTAP ) 和象 14 一样的 methyltransferase (METTL14 ) 的二个新部件。WTAP 与 METTL3 和 METTL14 交往,并且为他们的本地化被要求进在 vivo 与处理因素的 pre-mRNA 并且为 m6A methyltransferase 的催化活动充实的原子点缀。RNA 的多数在 vivo 由 WTAP 和 METTL3 跳了代表包含一致 m6A 主题的 mRNAs。当 WTAP 不在时, METTL3 的 RNA 有约束力的能力强烈被减少,建议 WTAP 可以工作调整到 mRNA 目标的 m6A methyltransferase 建筑群的招募。而且,在有 photoactivatable-ribonucleoside-enhanced crosslinking 和 immunoprecipitation (同等片断) 的联合的 transcriptomic 分析说明那 WTAP 和 METTL3 调整涉及抄写并且 RNA 处理的基因拼接的表示和选择。在 zebrafish 胚胎的调停 Morpholino 的击倒的指向 WTAP 或 METTL3 引起了织物区别缺点并且增加了 apoptosis。这些调查结果提供 WTAP 可以在 m6A methyltransferase 建筑群作为一个规章的子单元工作并且在 RNA 的 epitranscriptomic 规定起一个关键作用的充分证据新陈代谢。 | Xiao-Li Ping Bao-Fa Sun Lu Wang Wen Xiao Xin Yang Wen-Jia Wang Samir Adhikari Yue Shi Ying Lv Yu-Sheng Chen Xu Zhao Ang Li Ying Yang Ujwal Dahal Xiao-Min Lou Xi Liu Jun Huang Wei-Ping Yuan Xiao-Fan Zhu Tao Cheng Yong-Liang Zhao Xinquan Wang Jannie M Rendtlew Danielsen Feng Liu Yun-Gui Yang | 2014 | Cell Research2014，24，2： | 244 |
| 17 | Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘 | Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou | 2014 | Cell Research2014，24，6： | 81 |
| 18 | Honeysuckle-encoded atypical microRNA2911 directly Largets influenza A viruses显示文摘 | Zhen Zhou Xihan Li Jinxiong Liu Lei Dong Qun Chen Jialing Liu Huihui Kongt Qianyi Zhang Xian Qi Dongxia Hou Lin Zhang Guoquan Zhang Yuchen Liu Yujing Zhang Jing Li Jin Wang Xi Chen Hua Wang Junfeng Zhang Hualan Chen Ke Zen Chen-Yu Zhang | 2015 | Cell Research2015，25，1： | 104 |
| 19 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 20 | YTHDF3 facilitates translation and decay of N6-methyladenosine-modified RNA显示文摘 | Hailing Shi Xiao Wang Zhike Lu Boxuan S Zhao Honghui Ma Phillip J HSU Chang Liu Chuan He | 2017 | Cell Research2017，27，3： | 156 |
您所在的机构暂未开通此部分的功能，请联系管理员