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| 1 | Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity显示文摘The incorporation of hydroxyapatite(HAP)into poly-L-lactic acid(PLLA)matrix serving as bone scaffold is expected to exhibit bioactivity and osteoconductivity to those of the living bone.While too low degradation rate of HAP/PLLA scaffold hinders the activity because the embedded HAP in the PLLA matrix is difficult to contact and exchange ions with body fluid.In this study,biodegradable polymer poly(glycolic acid)(PGA)was blended into the HAP/PLLA scaffold fabricated by laser 3D printing to accelerate the degradation.The results indicated that the incorporation of PGA enhanced the degradation rate of scaffold as indicated by the weight loss increasing from 3.3%to 25.0%after immersion for 28 days,owing to the degradation of high hydrophilic PGA and the subsequent accelerated hydrolysis of PLLA chains.Moreover,a lot of pores produced by the degradation of the scaffold promoted the exposure of HAP from the matrix,which not only activated the deposition of bone like apatite on scaffold but also accelerated apatite growth.Cytocompatibility tests exhibited a good osteoblast adhesion,spreading and proliferation,suggesting the scaffold provided a suitable environment for cell cultivation.Furthermore,the scaffold displayed excellent bone defect repair capacity with the formation of abundant new bone tissue and blood vessel tissue,and both ends of defect region were bridged after 8 weeks of implantation. | Cijun Shuai Wenjing Yang Pei Feng Shuping Peng Hao Pan | 2021 | Bioactive Materials2021，6，2： | 8 |
| 2 | A review on application of dielectric barrier discharge plasma technology on the abatement of volatile organic compounds显示文摘Volatile organic compounds(VOCs)released from the waste treatment facilities have become a significant issue because they are not only causing odor nuisance but may also hazard to human health.Non-thermal plasma(NTP)technologies are newly developed methods and became a research trend in recent years regarding the removal of VOCs from the air stream.Due to its unique characteristics,such as rapid response at room temperature,bulk homogenized volume,high reaction efficiency,dielectric barrier discharge(DBD)plasma technology is considered one of the most promising techniques of NTP.This paper reviews recent progress of DBD plasma technology for abatement of VOCs.The principle of plasma generation in DBD and its configurations(electrode,discharge gap,dielectric barrier material,etc.)are discussed in details.Based on previously published literature,attention has been paid on the effect of DBD configuration on the removal of VOCs.Effect of various process parameters such as initial concentration,gas feeding rate,oxygen content and input power on VOCs removal are also considered.Moreover,the role of catalysis and inhibitors in VOCs removal by DBD system are presented.Finally,a modified configuration of the DBD reactor,i.e.double dielectric barrier discharge(DDBD)for the abatement of VOCs is discussed.It was suggested that the DDBD plasma reactor could be used for higher conversion efficiency as well as for avoiding solid residue deposition on the electrode.These depositions can interfere with the performance of the reactor. | Wenjing Lu Yawar Abbas Muhammad Farooq Mustafa Chao Pan Hongtao Wang | 2019 | Frontiers of Environmental Science & Engineering2019，13，2： | 6 |
| 3 | Spatiotemporal variations of nitrate sources and dynamics in a typical agricultural riverine system under monsoon climate显示文摘Nitrogen pollution is a serious environmental issue in the Danjiangkou Reservoir region(DRR),the water source of the South-to-North Water Diversion Project of China.In this research,seasonal surveys and a bi-weekly time series survey were conducted in the Qihe River Basin,one of the most densely populated agricultural basins in the DRR.Hydrochemical compositions(NO3^-Cl^-),dual isotopes(δD-H2O,δ^18O-H2O,δ^15N-NO3^-,andδ^18O-NO3^-),and a Markov Chain Monte Carlo isotope mixing model were jointly applied to unravel the sources,migrations,and transformations of the nitrate(NO3^-)in the basin.It was revealed that the mixing between different sources was the main process controlling the isotopic compositions of the riverine NO3the upper-middle reaches.In contrast,denitrification occurred in the lower reaches.For the first time,the sources of NO3^-quantified at a basin scale in the DRR.Overall,the river transported 484.2 tons/year of NO3^-N to the reservoir,of which 32.6%,36.4%,28.0%,and 3.0%was from soil organic nitrogen,chemical fertilizer,residential sewage and atmospheric precipitation,respectively.The NO3^-N fluxes of the different sources were regulated by the monsoon climate and anthropogenic activities.For example,high precipitation and intense fertilization resulted in severe nonpoint source pollution.Denitrification thrived in soils and reservoirs in wet seasons.Temperature could regulate the migration,nitrification and denitrification processes.Based on the results,we suggest that the management strategies dealing with nitrogen pollution issue in the DRR should follow the specific spatiotemporal characteristics of NO3migration and transformation mechanisms. | Hao Jiang Wenjing Liu Jiangyi Zhang Li Zhou Xiaode Zhou Ke Pan Tong Zhao Yuchen Wang Zhifang Xu | 2020 | Journal of Environmental Sciences2020，32，7： | 5 |
| 4 | A Fairness Resource Allocation Algorithm for Coverage and Capacity Optimization in Wireless Self-Organized Network显示文摘To achieve the higher resource efficiency, Coverage and Capacity Optimization(CCO) as an important role of the network self-healing and self-optimization, has become a focus topic in wireless Self-Organized Network(SON). In this paper, a novel CCO scheme is proposed to maximize utility function of the integrated coverage and capacity. It starts with the analysis on the throughput proportional fairness(PF) algorithm and then proposes the novel Coverage and Capacity Proportional Fairness(CCPF) allocation algorithm along with a proof of the algorithms convergence. This proposed algorithm is applied in a coverage capacity optimization scheme which can guarantee the reasonable network capacity by the coverage range accommodation. Next, we simulate the proposed CCO scheme based on telecom operators' real network data and compare with three typical resource allocation algorithms: round robin(RR), proportional fairness(PF) and max C/I. In comparison of the PF algorithm, the numerical results show that our algorithm increases the average throughput by 1.54 and 1.96 times with constructed theoretical data and derived real network data respectively. | Pan Zhao Lei Feng Peng Yu Wenjing Li Xuesong Qiu | 2018 | China Communications2018，15，11： | 5 |
| 5 | Confinement and antenna effect for ultrasmall Y_(2)O_(3):Eu^(3+) nanocrystals supported by MOF with enhanced near-UV light absorption thereby enhanced luminescence and excellently multifunctional applications显示文摘A novel host-guest luminous system with enhanced near-UV light absorption thereby enhanced luminescence are designed based on the synergism of quantum confinement,spatial confinement,and antenna effect,where ultrasmall Y_(2)O_(3):Eu^(3+)nanocrystals are fixed inside MOF(Eu/Y-BTC)as supporting structure.The Eu/Y-BTC not only limits the size and leads to lattice distortion of Y_(2)O_(3):Eu^(3+)nanocrystals and controls the distance between nanocrystals,but also promotes the light absorption and emission.The significantly red-shifted and broadened charge transfer band of Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)leads to the excellent applications of Y_(2)O_(3):Eu^(3+)in white light-emitting diodes(LEDs).Our results show that white light with superior color quality(CRI>90)and extremely high luminous efficacy(an LER of 335 lm/W)could be achieved using Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)as red phosphor.The Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)also improves the photoelectric performance of dye-sensitized solar cells(DSSCs),not only because Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)has a large specific surface area and the adsorption amount of the dye is increased,but also because the valence band position of Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)is 2.41 eV,which can provide an additional energy level between the TiO2 and dye,promoting electron transfer.For these advantageous features,the multifunctional Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)composite product will open new avenues in white LEDs and DSSCs. | Hongyi Xu Wenjing Yu Kai Pan Guofeng Wang Peifen Zhu | 2021 | Nano Research2021，14，3： | 5 |
| 6 | Corridor use by Asian elephants显示文摘There are 18 km of Kunming–Bangkok Highway passing through the Mengyang Nature Reserve of Xishuangbanna National Nature Reserve in Yunnan Province,China.From September 2005 to September 2006 the impact of this highway on movement of wild Asian elephants between the eastern and western part of the nature reserve was studied using track transecting,rural surveys and direct monitoring.Our results showed that the number of cross-road corridors used by Asian elephants diminished from 28 to 23 following the construction of the highway.In some areas,the elephant activity diminished or even disappeared,which indicated a change in their home ranges.The utilization rate of artificial corridors was 44%.We also found that elephants prefered artificial corridors that were placed along their original corridors.During the research,wild elephants revealed their adaptation to the highway.They were found walking across the highway road surface many times and for different reasons.We suggest that the highway management bureau should revise their management strategies to mitigate the potential risks caused by elephants on the road for the safety of the public and to protect this endangered species from harm.It is also very important to protect and maintain current Asian elephants corridors in this region. | Wenjing PAN Liu LIN Aidong LUO Li ZHANG | 2009 | Integrative Zoology2009，4，2： | 4 |
| 7 | Structural mechanism of cooperative activation of the human calcium-sensing receptor by Ca^(2+) ions and L-tryptophan显示文摘The human calcium-sensing receptor(CaSR)is a class C G protein-coupled receptor(GPCR)responsible for maintaining Ca^(2+)homeostasis in the blood.The general consensus is that extracellular Ca^(2+)is the principal agonist of CaSR.Aliphatic and aromatic L-amino acids,such as L-Phe and L-Trp,increase the sensitivity of CaSR towards Ca^(2+)and are considered allosteric activators.Crystal structures of the extracellular domain(ECD)of CaSR dimer have demonstrated Ca^(2+)and L-Trp binding sites and conformational changes of the ECD upon Ca^(2+)/L-Trp binding.However,it remains to be understood at the structural level how Ca^(2+)/L-Trp binding to the ECD leads to conformational changes in transmembrane domains(TMDs)and consequent CaSR activation.Here,we determined the structures of full-length human CaSR in the inactive state,Ca^(2+)-or L-Trp-bound states,and Ca^(2+)/L-Trp-bound active state using single-particle cryo-electron microscopy.Structural studies demonstrate that L-Trp binding induces the closure of the Venus flytrap(VFT)domain of CaSR,bringing the receptor into an intermediate active state.Ca^(2+)binding relays the conformational changes from the VFT domains to the TMDs,consequently inducing close contact between the two TMDs of dimeric CaSR,activating the receptor.Importantly,our structural and functional studies reveal that Ca^(2+)ions and L-Trp activate CaSR cooperatively.Amino acids are not able to activate CaSR alone,but can promote the receptor activation in the presence of Ca^(2+).Our data provide complementary insights into the activation of class C GPCRs and may aid in the development of novel drugs targeting CaSR. | Shenglong Ling Pan Shi Sanling Liu Xianyu Meng Yingxin Zhou Wenjing Sun Shenghai Chang Xing Zhang Longhua Zhang Chaowei Shi Demeng Sun Lei Liu Changlin Tian | 2021 | Cell Research2021，31，4： | 3 |
| 8 | Development of machine learning multi-city model for municipal solid waste generation prediction显示文摘Integrated management of municipal solid waste(MSW)is a major environmental challenge encountered by many countries.To support waste treatment/management and national macroeconomic policy development,it is essential to develop a prediction model.With this motivation,a database of MSW generation and feature variables covering 130 cities across China is constructed.Based on the database,advanced machine learning(gradient boost regression tree)algorithm is adopted to build the waste generation prediction model,i.e.,WGMod.In the model development process,the main influencing factors on MSW generation are identified by weight analysis.The selected key influencing factors are annual precipitation,population density and annual mean temperature with the weights of 13%,11%and 10%,respectively.The WGMod shows good performance with R^(2)=0.939.Model prediction on MSW generation in Beijing and Shenzhen indicates that waste generation in Beijing would increase gradually in the next 3–5 years,while that in Shenzhen would grow rapidly in the next 3 years.The difference between the two is predominately driven by the different trends of population growth. | Wenjing Lu Weizhong Huo Huwanbieke Gulina Chao Pan | 2022 | Frontiers of Environmental Science & Engineering2022，16，9： | 3 |
| 9 | Chinese SPRIGT realizes high temperature stability in the range of 5–25 K显示文摘The unit of thermodynamic temperature,the kelvin,is one of the seven base units of International System of Units.High accuracy measurements of thermodynamic temperature in the lowtemperature range,namely below the neon triple point at24.5561 K,are of strategic significance for the development of | Bo Gao Changzhao Pan Laurent Pitre Yanyan Chen Haiyang Zhang Yaonan Song Hui then Wenjing Liu Dongxu Han Ercang Luo | 2018 | Science Bulletin2018，63，12： | 3 |
| 10 | Fabrication and evaluation of a BMP-2/dexamethasone co-loaded gelatin sponge scaffold for rapid bone regeneration显示文摘Improving the osteogenic activity of BMP-2 in vivo has significant clinical application value.In this research,we use a clinical gelatin sponge scaffold loaded with BMP-2 and dexamethasone(Dex)to evaluate the osteogenic activity of dual drugs via ectopic osteogenesis in vivo.We also investigate the mechanism of osteogenesis induced by BMP-2 and Dex with C2C12,a multipotent muscle-derived progenitor cell.The results show that the gelatin scaffold with Dex and BMP-2 can significantly accelerate osteogenesis in vivo.It is indicated that compared with the BMP-2 or Dex alone,100nM of Dex can dramatically enhance the BMP-2-induced alkaline phosphatase activity(ALP),ALP mRNA expression and mineralization.Further studies show that 100nM of Dex can maintain the secondary structure of BMP-2 and facilitate recognition of BMP-2 with its receptors on the surface of C2C12 cells.We also find that in C2C12,Dex has no obvious effect on the BMP-2-induced Smad1/5/8 protein expression and the STAT3-dependent pathway,but Runx2-dependent pathway is involved in the Dex-stimulated osteoblast differentiation of BMP-2 both in vitro and in vivo.Based on these results,a potential mechanism model about the synergistic osteoinductive effect of Dex and BMP-2 in C2C12 cells via Runx2 activation is proposed.This may provide a theoretical basis for the pre-clinical application of Dex and BMP-2 for bone regeneration. | Qi Gan Hao Pan Wenjing Zhang Yuan Yuan Jiangchao Qian Changsheng Liu | 2022 | Regenerative Biomaterials2022，9，1： | 2 |
| 11 | MicroRNA-activated hydrogel scaffold generated by 3D printing accelerates bone regeneration显示文摘Bone defects remain a major threat to human health and bone tissue regeneration has become a prominent clinical demand worldwide.The combination of microRNA(miRNA)therapy with 3D printed scaffolds has always posed a challenge.It can mimic physiological bone healing processes,in which a biodegradable scaffold is gradually replaced by neo-tissue,and the sustained release of miRNA plays a vital role in creating an optimal osteogenic microenvironment,thus achieving promising bone repair outcomes.However,the balance between two key factors-scaffold degradation behavior and miRNA release profile-on osteogenesis and bone formation is still poorly understood.Herein,we construct a series of miRNA-activated hydrogel scaffolds(MAHSs)generated by 3D printing with different crosslinking degree to screened the interplay between scaffold degradation and miRNA release in the osteoinduction activity both in vitro and in vivo.Although MAHSs with a lower crosslinking degree(MAHS-0 and MAHS-0.25)released a higher amount of miR-29b in a sustained release profile,they degraded too fast to provide prolonged support for cell and tissue ingrowth.On the contrary,although the slow degradation of MAHSs with a higher crosslinking degree(MAHS-1 and MAHS-2.5)led to insufficient release of miR-29b,their adaptable degradation rate endowed them with more efficient osteoinductive behavior over the long term.MAHS-1 gave the most well-matched degradation rate and miR-29b release characteristics and was identified as the preferred MAHSs for accelerated bone regeneration.This study suggests that the bio-adaptable balance between scaffold degradation behavior and bioactive factors release profile plays a critical role in bone regeneration.These findings will provide a valuable reference about designing miRNAs as well as other bioactive molecules activated scaffold for tissue regeneration. | Ting Pan Wenjing Song Hongbao Xin Haiyue Yu He Wang Dandan Ma Xiaodong Cao Yingjun Wang | 2022 | Bioactive Materials2022，7，4： | 2 |
| 12 | The SWI/SNF chromatin-remodeling factors BAF60a, b, and c in nutrient signaling and metabolic control显示文摘新陈代谢的症候群成为了不利地影响人的健康的全球流行病。基因、环境的因素贡献新陈代谢的混乱的致病;然而,集成这些暗示到的机制调整新陈代谢的生理学和不完全地定义的新陈代谢的混乱遗体的发展。新兴的证据建议 SWI/SNF 改变染色质的建筑群为指导新陈代谢的 reprogramming 和改编响应是批评的营养并且另外的生理的信号。ATP 依赖的 SWI/SNF 改变染色质的建筑群包括多达 11 个子单元, BAF60 子单元在之中用作在核心建筑群和特定的 transcriptional 因素之间的一个关键连接。BAF60 子单元有三个成员, BAF60a, b,和 c。不同织物分发模式和 BAF60 蛋白质的规章的机制在不同新陈代谢的房间类型与专业化功能授与每 isoform。在这评论,我们在滋养的察觉到和精力新陈代谢在下面的规定总结 BAF60 蛋白质的新兴的角色和机制生理并且疾病条件。 | Ruo-Ran Wang Ran Pan Wenjing Zhang Junfen Fu Jiandie D. Lin Zhuo-Xian Meng | 2018 | Protein & Cell2018，9，2： | 2 |
| 13 | Responses of the Sea Surface Temperature and Suspended Matter Concentration to ENSO Events in the Bohai Sea显示文摘The sea surface temperature(SST)and sea surface suspended matter concentration data in the Bohai Sea were collected by multiple NOAA satellites from 1985 to 2018.The background calibration method was used to correct the systematic errors caused by the long-term observations through multiple NOAA satellites in this paper.Then,we studied the long-term variations of the sea surface temperature(SST)and sea surface suspended matter concentration(SSC)and their response to ENSO events in the Bohai Sea.Our results show that the SST and SSC of the Bohai Sea have been increasing since 1985,with an average rate of 0.04℃per year for SST,which is higher than the global average.The average increasing rate of SSC was 0.51 mg L^(-1)yr^(-1),which may be mainly due to the enhancement of winter monsoon in the Bohai.In winter,the SST and SSC of the Bohai Sea were higher in El Niño years than in La Niña years.In El Niño years,the ocean circulations were the main factor affecting the variation in SST.The robust circulation system caused by the strong winter monsoon enhances the water exchange between the cold water of the Bohai Sea and the warm water of the Yellow Sea,resulting in a higher SST in La Niña years.In summer,the SSTs of the Bohai Sea were lower in El Niño years than those in the La Niña years because of the decrease of the SST in the Western Pacific Ocean,the weakening of the subtropical high in the western Pacific,and its eastward shift caused by the El Niño events. | LIU Xue DING Dong LI Guangxue ZHANG Yang LIU Shidong WANG Nan ZHONG Yi YANG Xue PAN Yufeng YU Di ZHANG Lei XUE Wenjing | 2023 | Journal of Ocean University of China2023，22，3： | 1 |
| 14 | GmGPDH12,a mitochondrial FAD-GPDH from soybean, increases salt and osmotic stress resistance by modulating redox state and respiration显示文摘In plants,glycerol-3-phosphate dehydrogenase(GPDH)catalyzes the interconversion of glycerol-3-phosphate(G3P)and dihydroxyacetone phosphate(DHAP)coupled to the reduction/oxidation of the nicotinamide adenine dinucleotide(NADH)pool,and plays a central role in glycerolipid metabolism and stress response.Previous studies have focused mainly on the NAD+-dependent GPDH isoforms,neglecting the role of flavin adenine dinucleotide(FAD)-dependent GPDHs.We isolated and characterized three mitochondrialtargeted FAD-GPDHs in soybean,of which one isoform(GmGPDH12)showed a significant transcriptional response to NaCl and mannitol treatments,suggesting the existence of a major FAD-GPDH isoform acting in soybean responses to salt and osmotic stress.An enzyme kinetic assay showed that the purified GmGPDH12 protein possessed the capacity to oxidize G3P to DHAP in the presence of FAD.Overexpression and RNA interference of GmGPDH12 in soybean hairy roots resulted in elevated tolerance and sensitivity to salt and osmotic stress,respectively.G3P contents were significantly lower in GmGPDH12-overexpressing hair roots and higher in knockdown hair roots,indicating that GmGPDH12 was essential for G3P catabolism.A significant perturbation in redox status of NADH,ascorbic acid(ASA)and glutathione(GSH)pools was observed in GmGPDH12-knockdown plants under stress conditions.The impaired redox balance was manifested by higher reactive oxygen species generation and consequent cell damage or death;however,overexpressing plants showed the opposite results for these traits.GmGPDH12 overexpression contributed to maintaining constant respiration rates under salt or osmotic stress by regulating mRNA levels of key mitochondrial respiratory enzymes.This study provides new evidence for the roles of mitochondria-localized GmGPDH12 in conferring resistance to salt or osmotic stress by maintaining cellular redox homeostasis,protecting cells and respiration from oxidative injury. | Ying Zhao Xin Li Zexin Zhang Wenjing Pan Sinan Li Yun Xing Wanying Xin Zhanguo Zhang Zhenbang Hu Chunyan Liu Xiaoxia Wu Zhaoming Qi Dawei Xin Qingshan Chen | 2021 | The Crop Journal2021，9，1： | 1 |
| 15 | Investigation of High-Stability Temperature Control in Primary Gas Thermometry显示文摘We presented a relationship between the temperature control and measurement stability limit and the temperature resolution,particularly for using rhodium-iron resistance thermometers and AC resistance bridges.Based on this,temperature control was investigated and demonstrated in primary gas thermometry under various working conditions.With optimized parameters,micro-Kelvin level temperature control stability was realized in the temperature region from 5 K to 24.5 K.The temperature control stabilities are better than 8μK over 180 h with an integration time of 33.6 s in the concerned temperature range,closing to the limit that the sensors and the instruments can control and measure.These stabilities were significantly improved about(44±8)%at 24.5 K and(70±7)%at 5 K comparing with our previous work(Chen et al.,Cryogenics,2019,97:1–6). | HU Jiangfeng ZHANG Haiyang SONG Yaonan PAN Changzhao GAO Bo LIU Wenjing HAN Dongxu ZHANG Zhen LUO Ercang PITRE Laurent | 2022 | Journal of Thermal Science2022，31，3： | 1 |
| 16 | Magnetic mo- lecularly imprinted microcapsules derived from Picketing emulsion polymerization and their novel adsorption char- acteristics for h-cyhalothrin 显示文摘 | Jianming Pan Wenjing Zhu Xiaohui Dai | 2014 | RSC Adv2014，4，： | 1 |
| 17 | Molecular Engineering of Novel Fluorophores for High-Contrast Bioimaging显示文摘Fluorophores play a significant role in achieving high-contrast imaging in complex physiological environment.Herein,we present the results of our studies on the development of various fluorophores,such as anti-solvatochromic fluorophores,long wavelength emission two-photon fluorophores,large Stokes shift fluorophores and solid-state fluorophores,to achieve high-contrast bioimaging in physiological environments.in this study,we have primarily focused on the design,optical performance,imaging mechanisms,and the applications of these fluorophores with respect to complex physiological conditions. | Shuai Xu Wenjing Pan Tian-Bing Ren Shuang-Yan Huan Lin Yuan Xiao-Bing Zhang | 2022 | Chinese Journal of Chemistry2022，40，9： | 1 |
| 18 | DDG1 and G Protein α Subunit RGA1 Interaction Regulates Plant Height and Senescence in Rice(Oryza sativa)显示文摘Many studies have already shown that dwarfism and moderate delayed leaf senescence positively impact rice yield,but the underlying molecular mechanism of dwarfism and leaf senescence remains largely unknown.Here,using map-based cloning,we identified an allele of DEP2,DDG1,which controls plant height and leaf senescence in rice.The ddg1 mutant displayed dwarfism,short panicles,and delayed leaf senescence.Compared with the wild-type,ddg1 was insensitive to exogenous gibberellins(GA)and brassinolide(BR).DDG1 is expressed in various organs,especially in stems and panicles.Yeast two-hybrid assay,bimolecular fluorescent complementation and luciferase complementation image assay showed that DDG1 interacts with theα-subunit of the heterotrimeric G protein.Disruption of RGA1 resulted in dwarfism,short panicles,and darker-green leaves.Furthermore,we found that ddg1 and the RGA1 mutant was more sensitive to salt treatment,suggesting that DDG1 and RGA1 are involved in regulating salt stress response in rice.Our results show that DDG1/DEP2 regulates plant height and leaf senescence through interacting with RGA1. | Xi Liu Chuxuan Zhao Di Wang Gen Pan Xiaonan Ji Su Gao Tanxiao Du Yating Feng Wenjing Chen | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 1 |
| 19 | Analytical modeling of the junction evolution in single-molecule break junctions: towards quantitative characterization of the time-dependent process显示文摘The conductance through single-molecule junctions characterized by the break junction techniques consists of the through-space tunneling and through-molecule tunneling conductance, and the existence of through-space tunneling between the electrodes makes the quantitative extraction of the intrinsic molecular signals of single-molecule junctions challenging. Here, we established an analytic model to describe the evolution of the conductance of a single molecule in break junction measurements. The experimental data for a series of oligo(aryleneethynylene) derivatives validate the proposed model, which provides a modeling insight into the conductance evolution for the opening process in a 'real' break junction experiment. Further modulations revealed that the junction formation probability and rupture distance of the molecular junction, which reflect the junction stability, will significantly influence the amplitude and position of the obtained conductance peak. We further extend our model to a diffusion and a chemical reaction process, for which the simulation results show that the break junction technique offers a quantitative understanding of these time-dependent systems, suggesting the potential of break junction techniques in the quantitative characterization of physical and chemical processes at the single-molecule scale. | Zhi-Chao Pan Jin Li Lijue Chen Yongxiang Tang Jia Shi Junyang Liu Jie-Lou Liao Wenjing Hong | 2019 | Science China Chemistry2019，62，9： | 0 |
| 20 | Different conformational responses of theβ_(2)-adrenergic receptor-Gs complex upon binding of the partial agonist salbutamol or the full agonist isoprenaline显示文摘G protein-coupled receptors(GPCRs)are responsible for most cytoplasmic signaling in response to extracellular ligands with different efficacy profiles.Various spectroscopic techniques have identified that agonists exhibiting varying efficacies can selectively stabilize a specific conformation of the receptor.However,the structural basis for activation of the GPCR-G protein complex by ligands with different efficacies is incompletely understood.To better understand the structural basis underlying the mechanisms by which ligands with varying efficacies differentially regulate the conformations of receptors and G proteins,we determined the structures ofβ_(2)AR-Gαsβγbound with partial agonist salbutamol or bound with full agonist isoprenaline using single-particle cryo-electron microscopy at resolutions of 3.26?A and3.80?A,respectively.Structural comparisons between theβ_(2)AR-Gs-salbutamol andβ_(2)AR-Gs-isoprenaline complexes demonstrated that the decreased binding affinity and efficacy of salbutamol compared with those of isoprenaline might be attributed to weakened hydrogen bonding interactions,attenuated hydrophobic interactions in the orthosteric binding pocket and different conformational changes in the rotamer toggle switch in TM6.Moreover,the observed stronger interactions between the intracellular loop 2 or 3(ICL2 or ICL3)ofβ_(2)AR and Gαswith binding of salbutamol versus isoprenaline might decrease phosphorylation in the salbutamol-activatedβ_(2)AR-Gs complex.From the observed structural differences between these complexes ofβ_(2)AR,a mechanism ofβ_(2)AR activation by partial and full agonists is proposed to provide structural insights intoβ_(2)AR desensitization. | Fan Yang Shenglong Ling Yingxin Zhou Yanan Zhang Pei Lv Sanling Liu Wei Fang Wenjing Sun Liaoyuan A.Hu Longhua Zhang Pan Shi Changlin Tian | 2021 | National Science Review2021，8，9： | 0 |
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