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| 1 | Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity. | YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China | 2007 | Science China(Life Sciences)2007，50，2： | 4 |
| 2 | Side-chain modification of polyethylene glycol on conjugated polymers for ternary blend all-polymer solar cells with efficiency up to 9.27%显示文摘With the rapid progress achieved by all-polymer solar cells(all-PSCs), wide-bandgap copolymers have attracted intensive attention for their unique advantage of constructing complementary absorption profiles with conventional narrow-bandgap copolymers. In this work, we designed and synthesized a wide bandgap ternary copolymer PEG-2% which has the benzodithiophene-alt-difluorobenzotriazole as the backbone and the polyethylene glycol(PEG) modified side chain. The PBTA-PEG-2%:N2200 can be processed with a non-chlorinated solvent of 2-methyl-tetrahydrofuran(MeTHF) for the binary all-PSC, which exhibits a moderate photovoltaic performance. In particular, the ternary all-PSCs that consisting an additional narrow bandgap polymer donor PTB7-Th can also be processed with MeTHF, resulting in an unprecedented power conversion efficiency(PCE)of 9.27%, and a high PCE of 8.05% can be achieved with active layer thickness of 240 nm, both of which are the highest values so far reported from all-PSCs. Detailed investigations revealed that the dramatically improved device performances are attributable to the well-extended absorption band in the photoactive layer. Hence,developing novel copolymers with tailored side chains, and introducing additional polymeric components, can broaden the horizon for high-performance all-PSCs. | Zhenye Li Baobing Fan Baitian He Lei Ying Wenkai Zhong Feng Liu Fei Huang Yong Cao | 2018 | Science China Chemistry2018，61，4： | 4 |
| 3 | Crystallization Behavior and Kinetics of Lithium Aluminosilicate Glasses with Various Li_(2)O Contents显示文摘The crystallization behavior of Li_(2)O-Al_(2)O_(3)-SiO_(2) glasses with various concentration of Li2O were investigated by DSC,XRD,and FE-SEM.The h/l-quartz s.s.is the main crystalline phase for all of the glass with the heat treatment of 720℃/3 h+800℃/1.5 h while the crystallinity and crystal size increase with the increase of Li2O contents.Due to the negative thermal expansion coefficient of h/l-quartz s.s.,the thermal expansion coefficient of glass-ceramics decreases with the increasing of Li2O contents.When the Li2O content is 9mol%,the near-zero CTE20-700℃=-0.09×10^(-6)℃^(-1) is obtained upon the heat treatment of 720℃/3 h+800℃/1.5 h.The crystallization activation energy of the glass with 9mol%Li2O is about 351 kJ/mol,and the crystallization mechanism is volume crystallization. | 韩建军 HONG Wei 姜宏 HE Jianxiong WANG Qianchen WANG Jing GAO Wenkai | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，2： | 3 |
| 4 | Ultrasound guided neural stem cell transplantation through the lateral ventricle for treatment of cerebral palsy in children显示文摘A total of 24 children with cerebral palsy were enrolled in this study and underwent ultrasound guided transplantation of neural stem cells through the lateral ventricle. Neural stem cells (3.8 × 106-7.3 × 107) were injected into the lateral ventricles. Mild injury of lateral ventricular blood vessels occurred in only two cases (8.3%). Seven cases (29.2%) experienced a fever. Clinical manifestations were improved to varying degrees in eight cases (28.0%) within 3 months after transplantation. Patient condition did not worsen, and no patient experienced severe adverse reactions. | Sheng He Zuo Luan Suqing Qu Xuan Qiu Daqing Xin Wenkai Jia Yanhua Shen Zehui Yu Tao Xu | 2012 | Neural Regeneration Research2012，7，32： | 2 |
| 5 | Structure-based linker optimization of 6-(2-cyclohexyl-1-alkyl)-2-(2-oxo-2-phenylethylsulfanyl)pyrimidin-4(3H)-ones as potent non-nucleoside HIV-1 reverse transcriptase inhibitors显示文摘In continuation of our efforts toward the discovery of potent HIV-1 NNRTIs with diverse structures,a series of novel S-DACO analogues of 6-(2-cyclohexyl-1-allkyl)-2-(2-oxo-2-phenyl-ethylsulfanyl)pyrimidin-4(3 H)-ones were designed,synthesized and evaluated for their antiviral activities in MT-4 cells.Most of these new compounds showed moderate to good activities against wild type HIV-1 with IC_(50) values ranging from 7.55μmol/L to 0.018μmol/L.Among them,compound 5 c was identified as the most promising inhibitor against HIV-1 replication with an IC_(50)=0.018μmol/L,CC_(50)=194μmol/L,and SI=12791,which was much more potent than the reference drugs NVP and DLV and comparable to AZT and EFV.In addition,5 c also exhibited improved activity against double mutant HIV-1 strain RES056 compared to that of the reference drugs NVP/DLV and DB02.The preliminary structure-activity relationship(SAR)and molecular modeling studies were also discussed,which provides some useful indications for guiding the further rational design of new S-DACO analogues. | Daxiong Li Chunsheng Zhang Wei Ding Siming Huang Le Yu Nan Lu Wenkai Pan Yiming Li Erik De Clercq Christophe Pannecouque Hongbing Zhang Yueping Wang Yanping He Fener Chen | 2021 | Chinese Chemical Letters2021，32，3： | 1 |
| 6 | Optimal Expansion Planning Model for Integrated Energy System Considering Integrated Demand Response and Bidirectional Energy Exchange显示文摘The challenges of energy shortage and environmen-tal protection motivate people to take various measures to use energy wisely,and integrated energy systems are such a measure to tackle this challenge.In this paper,an optimal expansion planning model for an integrated energy system consisting of power grid,gas network and multiple energy hubs is proposed,where the planning objective is to minimize operational fuel cost and capital investment cost covering carbon capture equipment and energy hubs among others.To demonstrate the advantage of the proposed planning model,six case studies are investigated,and 13.47%annual cost savings can be achieved compared with the baseline planning scenario,which does not consider bidirectional energy exchange and integrated demand response program.Index Terms-Bidirectional energy exchange,energy hubs,integrated energy system,integrated demand response. | Wenkai Dong Zhigang Lu Liangce He Jiangfeng Zhang Tao Ma Xiaobo Cao | 2023 | CSEE Journal of Power and Energy Systems2023，9，4： | 0 |
| 7 | Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in Citrus sinensis显示文摘Citrus sinensis is the most cultivated and economically valuable Citrus species in the world,whose genome has been assembled by three generation sequencings.However,chromosome recognition remains a problem due to the small size of chromosomes,and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome.Here,we employ fluorescence in situ hybridization(FISH)with 9 chromosome painting probes,30 oligo pools,and 8 repetitive sequences to visualize 18 chromosomes.Then,we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3(CMA)staining.By this approach,we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions,which enables us to visualize significant differences between homologous chromosomes.Based on the map,we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site.In particular,we discover the translocation regions of chromosomes 4 and 9 in C.sinensis“Valencia.”The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. | Shipeng Song Hui Liu Luke Miao Li He Wenzhao Xie Hong Lan Changxiu Yu Wenkai Yan Yufeng Wu Xiao-peng Wen Qiang Xu Xiuxin Deng Chunli Chen | 2023 | Journal of Genetics and Genomics2023，50，6： | 0 |
| 8 | Tailoring Texture to Highly Strengthen AZ31 Alloy Plate in the Thickness Direction via Pre-tension and Rolling-Annealing显示文摘Conventional wrought Mg alloys,such as AZ31 and ZK60 rolled plates,usually exhibit significantly low tensile yield strength in the thickness direction.This can be attributed to the high activity of{10-12}tension twinning due to the strong basal texture(<0001>//ND,normal direction).In this work,the tensile yield strength in the ND of the as-rolled(AR)AZ31 plate increased from 50 to 150 MPa(increased by 200%)via simple processing,i.e.,pre-tension and rolling-annealing(PTRA)treatment.The strong basal texture(<0001>//ND)of the AR plate was changed into a weakened fiber texture(<0001>⊥ND).The evolution of microstructures during PTRA treatment and the activated deformation modes during uniaxial tension were studied quantitatively and statistically by the means of intergranular misorientation(IM)and in-grain misorientation axes(IGMA)analysis.The results indicate that various twin variants,as well as{10-12}-{10-12}secondary twins,were activated during pre-tension and rolling,and most residual matrix was consumed by twins after annealing.The dominated deformation modes in tension changed from{10-12}tension twinning(the AR sample)to prismatic slip(the PTRA sample)in the early tensile deformation.The underlying formation mechanism of the fiber texture and corresponding strengthening mechanism were discussed. | Tianjiao Li Jiang Zheng Lihong Xia Haoge Shou Yongfa Zhang Rong Shi Liuyong He Wenkai Li | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，2： | 0 |
| 9 | Highly Stretchable Ionic and Electronic Conductive Fabric显示文摘Wearable devices redefine the way people interact with machines.Despite the intensive effort in the design and fabrication of synthetic fibers to improve wearable device properties in terms of electronic and ionic conductivity,stretchability,com-fort,and washability,challenges remain in fabricating single fiber materials that optimize all properties simultaneously.In this work,we demonstrate a highly stretchable,ionic,and electronic conductive fabric via(1)the natural nanoscale chan-nels in fibers for effective ion transportation,(2)confining the electronic conductive material with the cellulose fibers,and(3)decoupling the property degradation of the fiber from deformation using the knitted pattern.The hierarchical structure created by cotton fibers can serve as ionic conductive channels as well as a robust multiscale scaffold to host infiltrated elec-tronic conductive materials.Cotton strands with ionic and electronic conductivity can be knitted into fabrics that are highly stretchable(~300%).Moreover,high ionic and electronic conductivity are observed with 2 S/m and 5 S/m,respectively,even under a strain of 175%.With the inherent advantages of cotton fabrics such as moisture-wicking,washability,comfort,and light-weightiness for wearable applications,our approach of directly functionalized cellulose can potentially be a promising route towards highly stretchable and wearable mixed conductors. | Pengfei Deng Xuan Li Yanbin Wang Zijian He Wenkai Zhu Yun Zhang Gabriella M.Schalm Tian Li | 2023 | Advanced Fiber Materials2023，5，1： | 0 |
| 10 | Melatonin inhibits Gram-negative pathogens by targeting citrate synthase显示文摘Bacterial infections caused by Gram-negative pathogens represent a growing burden for public health worldwide.Despite the urgent need for new antibiotics that effectively fight against pathogenic bacteria,very few compounds are currently under development or approved in the clinical setting.Repurposing compounds for other uses offers a productive strategy for the development of new antibiotics.Here we report that the multifaceted melatonin effectively improves survival rates of mice and decreases bacterial loads in the lung during infection.Mechanistically,melatonin specifically inhibits the activity of citrate synthase of Gram-negative pathogens through directly binding to the R300,D363,and H265 sites,particularly for the notorious Pasteurella multocida.These findings highlight that usage of melatonin is a feasible and alternative therapy to tackle the increasing threat of Gram-negative pathogen infections via disrupting metabolic flux of bacteria. | Fang He Yuan Liu Pan Li Xiaoyan Wu Yaoyao Xia Dong Zhang Nengzhang Li Yuanyi Peng Guoqiang Zhu Rüdiger Hardeland Russel JReiter Wenkai Ren | 2022 | Science China(Life Sciences)2022，65，7： | 0 |
| 11 | Attention-based efficient robot grasp detection network显示文摘To balance the inference speed and detection accuracy of a grasp detection algorithm,which are both important for robot grasping tasks,we propose an encoder–decoder structured pixel-level grasp detection neural network named the attention-based efficient robot grasp detection network(AE-GDN).Three spatial attention modules are introduced in the encoder stages to enhance the detailed information,and three channel attention modules are introduced in the decoder stages to extract more semantic information.Several lightweight and efficient DenseBlocks are used to connect the encoder and decoder paths to improve the feature modeling capability of AE-GDN.A high intersection over union(IoU)value between the predicted grasp rectangle and the ground truth does not necessarily mean a high-quality grasp configuration,but might cause a collision.This is because traditional IoU loss calculation methods treat the center part of the predicted rectangle as having the same importance as the area around the grippers.We design a new IoU loss calculation method based on an hourglass box matching mechanism,which will create good correspondence between high IoUs and high-quality grasp configurations.AEGDN achieves the accuracy of 98.9%and 96.6%on the Cornell and Jacquard datasets,respectively.The inference speed reaches 43.5 frames per second with only about 1.2×10^(6)parameters.The proposed AE-GDN has also been deployed on a practical robotic arm grasping system and performs grasping well.Codes are available at http://gffzz188fe103f8f1460asnofnw6qn9f9p6pqc.ffgz.tsg.suse.edu.cn/robvincen/robot_gradet. | Xiaofei QIN Wenkai HU Chen XIAO Changxiang HE Songwen PEI Xuedian ZHANG | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，10： | 0 |
| 12 | Chip design with machine learning:a survey from algorithm perspective显示文摘Chip design with machine learning(ML)has been widely explored to achieve better designs,lower runtime costs,and no human-in-the-loop process.However,with tons of work,there is a lack of clear links between the ML algorithms and the target problems,causing a huge gap in understanding the potential and possibility of ML in future chip design.This paper comprehensively surveys existing studies in chip design with ML from an algorithm perspective.To achieve this goal,we first propose a novel and systematical taxonomy that divides target problems in chip design into three categories.Then,to solve the target problems with ML algorithms,we formulate the three categories as three ML problems correspondingly.Based on the taxonomy,we conduct a comprehensive survey in terms of target problems based on different ML algorithms.Finally,we conclude three key challenges for existing studies and highlight several insights for the future development of chip design with machine learning.By constructing a clear link between chip design problems and ML solutions,we hope the survey can shed light on the road to chip design intelligence from previous chip design automation. | Wenkai HE Xiaqing LI Xinkai SONG Yifan HAO Rui ZHANG Zidong DU Yunji CHEN | 2023 | Science China(Information Sciences)2023，66，11： | 0 |
| 13 | Reflective ultrathin light-sheet microscopy with isotropic 3D resolutions显示文摘Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy. | YUE WANG DASHAN DONG WENKAI YANG RENXI HE MING LEI KEBIN SHI | 2024 | Photonics Research2024，12，2： | 0 |
| 14 | Phenylalanine diminishes M1 macrophage inflammation显示文摘Emerging evidence suggests that amino acids dictate the effector functions of immune cells;however, whether and how phenylalanine(Phe) orchestrates the polarization of macrophages is not understood. Here, we determined that Phe attenuated lipopolysaccharide(LPS) and P. multocida serotype A strain CQ2(PmCQ2) infection-induced inflammation in vivo. Furthermore, we demonstrated that Phe inhibited the production of interleukin(IL)-1β and tumor necrosis factor(TNF)-α in proinflammatory(M1) macrophages. Phe reprogrammed the transcriptomic and metabolic profiles and enhanced oxidative phosphorylation in M1 macrophages, which reduced the activation of caspase-1. Notably, the valine-succinyl-CoA axis played a critical role in Phe-mediated inhibition of IL-1β production in M1 macrophages. Taken together, our findings suggest that manipulating the valine-succinyl-CoA axis provides a potential target for preventing and/or treating macrophage-related diseases. | Qingzhuo Zhang Siyuan Chen Yan Guo Fang He Jian Fu Wenkai Ren | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 15 | Gut microbiota bridges dietary nutrients and host immunity显示文摘Dietary nutrients and the gut microbiota are increasingly recognized to cross-regulate and entrain each other,and thus affect host health and immune-mediated diseases.Here,we systematically review the current understanding linking dietary nutrients to gut microbiota-host immune interactions,emphasizing how this axis might influence host immunity in health and diseases.Of relevance,we highlight that the implications of gut microbiota-targeted dietary intervention could be harnessed in orchestrating a spectrum of immune-associated diseases. | Lijuan Fan Yaoyao Xia Youxia Wang Dandan Han Yanli Liu Jiahuan Li Jie Fu Leli Wang Zhending Gan Bingnan Liu Jian Fu Congrui Zhu Zhenhua Wu Jinbiao Zhao Hui Han Hao Wu Yiwen He Yulong Tang Qingzhuo Zhang Yibin Wang Fan Zhang Xin Zong Jie Yin Xihong Zhou Xiaojun Yang Junjun Wang Yulong Yin Wenkai Ren | 2023 | Science China(Life Sciences)2023，66，11： | 0 |
| 16 | Rational Design of Plasmonic Metal Nanostructures for Solar Energy Conversion显示文摘Plasmonic metal nanostructures,possessing unique surface plasmon resonance properties,show excellent capabilities for light trapping and coupling.On this basis,various plasmonic metal nanostructures offer extraordinary opportunities to promote the conversion efficiency of solar energy to electric energy,hydrogen energy or thermal energy,and so on.In this review article,we highlight a number of recent research achievements on the rational design of plasmonic metal nanostructures so as to maximize the utilization of the entire solar spectrum.Compared with single metal nanoparticles,multiplex(such as multicompositions,sizes,or shapes)nanoparticle structures emphasize advantages in broadening the absorption range and improving light-utilization efficiency.This review concludes with discussions regarding challenges in this research field and proposals of prospects for future directions. | Yawen Wang Junchang Zhang Wenkai Liang He Yang Tianfu Guan Bo Zhao Yinghui Sun Lifeng Chi Lin Jiang | 2022 | CCS Chemistry2022，4，4： | 0 |
| 17 | Correlation between clinical course and radiographic development on CT scan in patients with COVID-19显示文摘Background:To analyze the correlation between clinical course and radiographic development on computed tomography(CT)in patients with confirmed coronavirus disease 2019(COVID-19)and to provide more evidence for treatment.Methods:This retrospective,observational,cohort study enrolled 49 patients with Reverse transcription-polymerase chain reaction(RT-PCR)-confirmed COVID-19,which included 30 patients admitted to the intensive care unit(ICU)of Wuhan Third Hospital and 19 patients either admitted to or receiving telemedicine consultation from Shanghai General Hospital,Shanghai Xuhui Dahua Hospital,and hospitals in other provinces.CT scans were performed in all enrolled patients and the radiographic features including simple ground-glass opacities(GGOs),GGO with interlobular septal thickening,consolidations with GGO,and consolidations only were monitored by repeating the CT.The progression of these radiographic features was analyzed in combination with their clinical staging and the time interval between onset of symptoms to CT.Results:Based on illness severity,the 49 patients were classified into four stages:mild(n=6),moderate(n=12),severe(n=16),and critically ill(n=15).The CT findings were classified into three phases:early(n=5),progres-sion(n=39),and recovery(n=5).Among the 49 patients,9 had bilateral diffuse GGO or diffuse consolidations(white lungs)and were counted as 18 lesions.Three patients had no abnormal findings on initial CT,but their repeat CT showed new lesions.In all,we identified 892 lesions including simple GGO,GGO with interlobular septal thickening,consolidations with GGO,and consolidations only.Conclusions:Most patients had pulmonary lesions on the posterior,inferior,and peripheral lung fields on CT.The development of GGO with interlobular septal thickening,GGO with consolidations,and consolidations only happened mainly between day 8 and 14.The emergence of consolidations may suggest the progression to the severe phase of the illness,whereas simple consolidations or“white lung”may suggest a critically ill phase. | Yun Xie Luyu Yang Hui Dong Song Cao Wenkai Zhang Qiong Chen Baoliang Jin Zhiguo Zhou Jun Liu Zhiyan He Ruilan Wang | 2021 | Journal of Intensive Medicine2021，1，1： | 0 |
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