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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Palaeoproterozoic Khondalite Belt in the western North China Craton: New evidence from SHRIMP dating and Hf isotope composition of zircons from metamorphic rocks in the Bayan Ul-Helan Mountains area显示文摘Zircom U-Pb age and Hf isotope analyses were made on gneissic granite and garnet-mica two-feldspar gneiss from the Helanshan Group in the Bayan Ul-Helan Mountains area, the western block of the North China Craton (NCC). Zircons from the gneissic granite commonly show core-mantle-rim structures, with magmatic core, metamorphic mantle and rim having ages of 2323±20 Ma, 1923±28 Ma and 1856±12 Ma, respectively. The core, mantle and rim show similar Hf isotope compositions, with single-stage depleted mantle model ages (TDM1) of 2455 to 2655 Ma (19 analyses). Most of the detrital zircons from the garnet-mica two-feldspar paragneiss have a concentrated U-Pb age distribution, with a weighted mean 207Pb/206Pb age of 1978±17 Ma. A few detrital zircons are older (2871 to 2469 Ma). The age for metamorphic overgrown rim was not determined because of strong Pb loss due to their high U content. The zircons show large variation in Hf isotope composition, with TDM1 ages of 1999 to 3047 Ma. In com- bination with previous studies, the main conclusions are as follows: (1) protolith of the khondalite se- ries in the Helanshan Group formed during Palaeoproterozoic rather than the Archaean as previously considered; (2) The results lend support to the contention that there is a huge Palaeoproterozoic Khondalite (metasedimentary) Belt between the Yinshan Mountains Block and the Ordos Block in the Western Block of NCC; (3) The widely-distributed bodies of early Palaeoproterozoic orthogneisses in the Khondalite Belt might be one of the important sources for detritus material in the khondalite series; (4) Collision between the Yinshan Block, the Ordos Block and the Eastern Block occurred in the same tectonothermal event of late Palaeoproterozoic, resulting in the final assembly of the NCC. | DONG ChunYan LIU DunYi LI JunJian WAN YuSheng ZHOU HongYing LI ChengDong YANG YueHeng XIE LieWen | 2007 | Chinese Science Bulletin2007，52，21： | 52 |
| 3 | Chemical composition of PM_(2.5) during winter in Tianjin,China显示文摘PM2.5 samples for 24 h were collected during winter in Tianjin,China. The ambient mass concentration and chemical composition of the PM2.5 were determined. Ionic species were analyzed by ion chromatog-raphy,while carbonaceous species were determined with the IMPROVE thermal optical reectance(TOR) method,and inorganic elements were measured by inductively coupled plasma-atomic emission spec-trometer. The daily PM2.5 mass concentrations ranged from 48.2 to 319.2 g/m3 with an arithmetic average of 144.6 g/m3. The elevated PM2.5 in winter was mostly attributed to combustion sources such as vehicle exhaust,heating,cooking and industrial emissions,low wind speeds and high relative humidity(RH) ,which were favorable for pollutant accumulation and formation of secondary pollutants. By chem-ical mass balance,it was estimated that about 89.1% of the PM2.5 mass concentrations were explained by carbonaceous species,secondary particles,crustal matters,sea salt and trace elements. Organic material was the largest contributor,accounting for about 32.7% of the total PM2.5 mass concentrations. SO42-,NO3-,Cl- and NH4+ were four major ions,accounting for 16.6%,11.5%,4.7% and 6.0%,respectively,of the total mass of PM2.5. | Jinxia Gu Zhipeng Bai Weifang Li Liping Wu Aixia Liu Haiyan Dong Yiyang Xie | 2011 | Particuology2011，9，3： | 60 |
| 4 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 5 | A Rare Allele of GS2 Enhances Grain Size anc Grain Yield in Rice显示文摘谷物尺寸决定谷物重量并且影响谷物质量。调整谷物尺寸的几主要量的特点 loci (QTL ) 被克隆;然而,我们调整米饭谷物的尺寸的内在的机制的理解仍然保持碎片。这里,我们报导克隆和主导的 QTL 的描述,染色体 2 上的谷物尺寸(GS2 ) 编码调整生长的因素 4,一个 transcriptional 管理者。GS2 本地化到原子核并且可以充当抄写使活跃之物。影响 microRNA 的有约束力的地点的 GS2 的一个稀罕变化, OsmiR396c,原因提高了 GS2/OsGRF4 的表示。GS2 表示的增加导致更大的房间和房间的增加的数字,它因此提高谷物重量和产量。进米饭栽培变种的 GS2/OsGRF4 的这稀罕等位基因的介绍能显著地提高谷物重量和增加谷物产量,用在引起产量很高的米饭变化的可能的应用。 | Jiang Hu Yuexing Wang Yunxia Fang Longjun Zeng Jie Xu Haiping Yu Zhenyuan Shi Jiangjie Pan Dong Zhang Shujing Kang Li Zhu Guojun Dong Longbiao Guo Dali Zeng Guangheng Zhang Lihong Xie Guosheng Xiong Jiayang Li Qian Qian | 2015 | Molecular Plant2015，8，10： | 57 |
| 6 | SHRIMP zircon U-Pb dating of late Paleoproterozoic kondalites in the Daqing Mountains area on the North China Craton显示文摘The Daqing Mountains area comprises a typical occurrence of the Khondalite Belt in the Western Block of the North China Craton (NCC). In this area, both early and late Paleoproterozoic metasedimentary rocks have been identified in what was originally called the Upper Wula Mountains 'Subgroup'. Six metasedimentary rock samples yielded SHRIMP U-Pb zircon ages of 2.56-2.04 Ga for detrital and 1.96-1.83 Ga for metamorphic zircons. Based on these data and previously published results, the following conclusions can be drawn: 1) The source region for the late Paleoproterozoic detrital sedimentary rocks is mainly 2.55 2.4 and 2.2 2.04 Ga in age, consistent with the early Precambrian geological history identified widely in the basement of the NCC. 2) The majority of sedimentary rocks of the khondalite series were deposited between 2.04 and 1.95 Ga, and then in a protracted period (1.96 and 1.83 Ga) underwent a complex history of amphibolite to granulite-facies metamorphism. | DONG ChunYan WAN YuSheng XU ZhongYuan LIU DunYi YANG ZhenSheng MA MingZhu XIE HangQiang | 2013 | Science China Earth Sciences2013，56，1： | 43 |
| 7 | Archean Basement and a Paleoproterozoic Collision Orogen in the Huoqiu Area at the Southeastern Margin of North China Craton: Evidence from Sensitive High Resolution Ion Micro-Probe U-Pb Zircon Geochronology显示文摘这份报纸报导微探查的 U-Pb 锆石在 Huoqiu 区域为 Huoqiu 组和早前寒武纪的地下室的 granitoids 变老的敏感高分辨率离子,北方中国 Craton 的东南的边缘。Huoqiu 组在岩石协会和变态是类似的到 khondalite 系列,除了它包含 banded 铁形成的可观的数量。从 Huoqiu 的所有由岩屑形成的锆石组织元沉积的岩石是 3.0 Ga 和 2.75 Ga,没有任何 2.5 Ga 和更年轻的,作为通常在北方中国 Craton 的另外的区域在 Paleoproterozoic khondalite 系列被发现。在 Huoqiu 区域, 2.75 Ga 和 2.56 Ga granitoids 也被识别了。这个地下室集合在迟了的 Paleoproterozoic (1.84 Ga ) 期间经历了强壮的变态广泛地在北方中国 Craton 被开发的 tectonothermal 事件。因此, Huoqiu 组的形成时间能以由岩屑形成、变形的锆石年龄在 2.75 和 1.84 Ga 之间被抑制。它被考虑,与地区性的数据结合了,可以有 Paleoproterozoic 碰撞 orogen,在一个 NWWSEE 方向延长到北方中国 Craton 的南部的边缘。 | WAN Yusheng DONG Chunyan WANG Wei XIE Hangqiang LIU Dunyi | 2010 | Acta Geologica Sinica(English Edition)2010，84，1： | 35 |
| 8 | Detrital zircon age model of Ordovician Wenquan quartzite south of Lungmuco-Shuanghu Suture in the Qiangtang area, Tibet: Constraint on tectonic affinity and source regions显示文摘Early to Middle Ordovician strata, including Wenquan quartzite, occur widely in the Himalaya, Lhasa, and south Qiangtang blocks. The Wenquan quartzite occurs on the south side of the Lungmuco-Shuanghu Suture in the Qiangtang area, Tibet. A total of 145 analyses on detrital zircons from the quartzite show five age ranges of 520-700, ca. 800, 900-1100, 1800-1900, and 2400-2500 Ma, with particularly distinct age peaks of 625 and 950 Ma. The reliable youngest detrital zircon age is 525 Ma, and the oldest, 3180 Ma. Detrital zircons show large variations in Hf isotope composition, with depleted mantle model ages t DM (Hf) ranging from 750 to 3786 Ma. Based on data obtained in this study and by others, the main conclusions are as follows: 1) Low-grade metamorphic sedimentary rocks are distributed extensively in the south of the Lungmuco-Shuanghu Suture and are Phanerozoic in age; 2) Pan-African and Grenville-Jinning tectono-thermal events were well developed in the source region of the Wenquan quartzite; 3) the source region shows crustal addition and recycling of different periods; 4) Wenquan quartzite was derived from the Gondwana metamorphic basement, suggesting that the Qiangtang block is a Gondwanan fragment. | DONG ChunYan LI Cai WAN YuSheng WANG Wei WU YanWang XIE HangQiang LIU DunYi | 2011 | Science China Earth Sciences2011，54，7： | 39 |
| 9 | Practice guidelines for ultrasound-guided percutaneous microwave ablation for hepatic malignancy显示文摘Primary liver cancer and liver metastases are among the most frequent malignancies worldwide,with an increasing number of new cases and deaths every year.Traditional surgery is only suitable for a limited proportion of patients and imaging-guided percutaneous thermal ablation has achieved optimistic results for management of hepatic malignancy.This synopsis outlines the first clinical practice guidelines for ultrasoundguided percutaneous microwave ablation therapy for hepatic malignancy,which was created by a joint task force of the Society of Chinese Interventional Ultrasound.The guidelines aim at standardizing the microwave ablation procedure and therapeutic efficacy assessment,as well as proposing the criteria for the treatment candidates. | Ping Liang Jie Yu Ming-De Lu Bao-Wei Dong Xiao-Ling Yu Xiao-Dong Zhou Bing Hu Ming-Xing Xie Wen Cheng Wen He Jian-Wen Jia Guo-Rong Lu | 2013 | World Journal of Gastroenterology2013，19，33： | 26 |
| 10 | Spinal surgeons' learning curve for lumbar microendoscopic discectomy: a prospective study of our first 50 and latest 10 cases显示文摘背景 Microendoscopic discectomy ( MED )是为腰部的圆盘形成疝从外科允许快速的恢复的最低限度地侵略的操作,但是因为大多数外科医生避免它 curve.We 在针的 surgeons'learning curve.Methods 的阶段评估了腰部的 MED 的有效性和安全的长学习,在很少医院里代替了传统的开的外科从2002年6月接待 MED 到2003年2月的五十个病人各被划分成十的按年代先后的组: A-E.The 控制组 F 是十 | RONG Li-min XIE Pei-gen SHI De-hai DONG Jian-wen LIU Bin FENG Feng CAI Dao-zhang | 2008 | Chinese Medical Journal2008，，21： | 23 |
| 11 | Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize显示文摘 | Le Dong Lina Li Changlin Liu Chenxu Liu Shuaifeng Geng Xinhai Li Changling Huang Long Mao Shaojiang Chen Chuanxiao Xie | 2018 | Molecular Plant2018，11，9： | 22 |
| 12 | The second natural gas hydrate production test in the South China Sea显示文摘Clayey silt reservoirs bearing natural gas hydrates(NGH)are considered to be the hydrate-bearing reservoirs that boast the highest reserves but tend to be the most difficult to exploit.They are proved to be exploitable by the first NGH production test conducted in the South China Sea in 2017.Based on the understanding of the first production test,the China Geological Survey determined the optimal target NGH reservoirs for production test and conducted a detailed assessment,numerical and experimental simulation,and onshore testing of the reservoirs.After that,it conducted the second offshore NGH production test in 1225 m deep Shenhu Area,South China Sea(also referred to as the second production test)from October 2019 to April 2020.During the second production test,a series of technical challenges of drilling horizontal wells in shallow soft strata in deep sea were met,including wellhead stability,directional drilling of a horizontal well,reservoir stimulation and sand control,and accurate depressurization.As a result,30 days of continuous gas production was achieved,with a cumulative gas production of 86.14×104 m3.Thus,the average daily gas production is 2.87×10^4 m^3,which is 5.57 times as much as that obtained in the first production test.Therefore,both the cumulative gas production and the daily gas production were highly improved compared to the first production test.As indicated by the monitoring results of the second production test,there was no anomaly in methane content in the seafloor,seawater,and atmosphere throughout the whole production test.This successful production test further indicates that safe and effective NGH exploitation is feasible in clayey silt NGH reservoirs.The industrialization of hydrates consists of five stages in general,namely theoretical research and simulation experiments,exploratory production test,experimental production test,productive production test,and commercial production.The second production test serves as an important step from the exploratory production test to experimental production test. | Jian-liang Ye Xu-wen Qin Wen-wei Xie Hai-long Lu Bao-jin Ma Hai-jun Qiu Jin-qiang Liang Jing-an Lu Zeng-gui Kuang Cheng Lu Qian-yong Liang Shi-peng Wei Yan-jiang Yu Chunsheng Liu Bin Li Kai-xiang Shen Hao-xian Shi Qiu-ping Lu Jing Li Bei-bei Kou Gang Song Bo Li He-en Zhang Hong-feng Lu Chao Ma Yi-fei Dong Hang Bian | 2020 | China Geology2020，3，2： | 22 |
| 13 | The precipitation strengthening behavior of Cu-rich phase in Nb contained advanced Fe-Cr-Ni type austenitic heat resistant steel for USC power plant application | Cheng-yu Chi a, Hong-yao Yu b, Jian-xin Dong b, Wen-qing Liu c, Shi-chang Cheng d, Zheng-dong Liu d, Xi-shan Xie b a School of Metallurgical and Ecological Engineering, University of Science & Technology Beijing, Beijing 100083, China b School of Materials Science and Engineering, University of Science & Technology Beijing, Beijing 100083, China c Instrumental Analysis & Research Center, Shanghai University, Shanghai 200444, China d Central Iron and Steel Research Institute, Beijing 100081, China | 2012 | Progress in Natural Science:Materials International2012，22，3： | 20 |
| 14 | Serum uric acid levels correlate with recurrence of paroxysmal atrial fibrillation after catheter ablation显示文摘 | HE Xiao-nan LI Song-nan ZHAN Jin-liang XIE Shuang-lun ZHANG Zhi-jun DONG Jian-zeng YU Rong-hui LONG De-yong TANG Ri-bo MA Chang-sheng | 2013 | Chinese Medical Journal2013，，5： | 18 |
| 15 | Clinical efficacy of 0.1% pranoprofen in treatment of dry eye patients:a multicenter, randomized, controlled clinical trial显示文摘 | Chen Jingyao Dong Fei Chen Wei Sun Xuguang Deng Yingping Hong Jing Zhang Mingchang Yang Wenzhao Liu Zuguo Xie Lixin | 2014 | Chinese Medical Journal2014，，13： | 18 |
| 16 | Intercropping with Solanum nigrum and Solanum photeinocarpum from Two Ecoclimatic Regions Promotes Growth and Reduces Cadmium Uptake of Eggplant Seedlings显示文摘Soil contamination by heavy metals is a serious environmental problem worldwide, and reduction of heavy metal accumulation in vegetables grown on contaminated land is a matter of urgency. A pot experiment was conducted to study the effects of intercropping with the Cd hyperaccumulators Solanum nigrum and Solanum photeinocarpum from two ecoclimatic regions, Ya'an and Chengdu,Sichuan Province, China, on the growth and cadmium(Cd) uptake of eggplant(Solanum melongena L.). The biomass, photosynthetic pigment contents, and activities of antioxidant enzymes of eggplant were enhanced by intercropping. The biomass of eggplant was the highest after intercropping with S. photeinocarpum from Ya'an, but did not differ significantly from that after intercropping with S. nigrum from Chengdu. The shoot Cd content of eggplant was significantly reduced by intercropping with the hyperaccumulators,which ranked as follows: S. nigrum from Chengdu > S. nigrum from Ya'an > S. photeinocarpum from Chengdu > S. photeinocarpum from Ya'an, with the decreases being 19.60%, 14.36%, 9.66%, and 6.42%, respectively, as compared with the control. The lowest shoot Cd content and translocation factor of eggplant were attained after intercropping with S. nigrum from Chengdu. Therefore, it was feasible to intercrop eggplant with S. nigrum and S. photeinocarpum on Cd-contaminated soil. | TANG Yi HE Juan YU Xuena XIE Yongdong LIN Lijin SUN Guochao LI Huanxiu LIAO Mingan LIANG Dong XIA Hui WANG Xun ZHANG Jing LIU Zejing TU Lihua LIU Li | 2017 | Pedosphere2017，27，3： | 17 |
| 17 | Economic Dispatch of Power Systems with Virtual Power Plant Based Interval Optimization Method显示文摘Load prediction and power prediction uncertainties are inevitable aspects of a virtual power plant(VPP).In power system economic dispatch(ED)modeling,the interval is used to describe prediction uncertainties.An ED model with interval uncertainty is established in this paper.The probability degree definition is adopted to convert the interval-based economic dispatch model into a deterministic model for the purposes of solving the modeling problem.Simulation tests are performed on a 10-machine system using professional optimization software(LINGO).The simulation results verify the validity of the proposed interval-based scheme for the economic dispatch of a power system with VPP. | Chongxin Huang Dong Yue Jun Xie Yaping Li Ke Wang | 2016 | CSEE Journal of Power and Energy Systems2016，2，1： | 17 |
| 18 | 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages显示文摘5-Hydroxymethylcytosine (5hmC ) 是被连接了到基因规定和癌症致病的重要哺乳动物的 DNA epigenetic 修正。这里,我们用定序途径的一枝敏感化学基于标签的低输入的猎枪在传播没有房间的 DNA (cfDNA ) 探索了 5hmC 的诊断潜力。我们从七种不同癌症类型的 49 个病人定序没有房间的 5hmC 并且发现了能被用来与高精确性预言癌症类型和阶段的不同特征。明确地,我们发现肺癌症在 cfDNA 导致 5hmC 的进步全球损失,而 hepatocellular 癌和胰腺的癌症在没有房间的 hydroxymethylome 导致疾病特定的变化。我们的 proof-of-principle 结果建议没有房间的 5hmC 签名可以潜在地被使用不仅识别癌症类型而且在一些癌症追踪肿瘤舞台。 | Chun-Xiao Song Senlin Yin Li Ma Amanda Wheeler Yu Chen Yan Zhang Bin Liu Junjie Xiong Weihan Zhang Jiankun Hu Zongguang Zhou Biao Dong Zhiqi Tian Stefanie S Jeffrey Mei-Sze Chua Samuel So Weimin Li Yuquan Wei Jiajie Diao Dan Xie Stephen R Quake | 2017 | Cell Research2017，27，10： | 17 |
| 19 | Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice显示文摘AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the survival rate of acute liver failure(ALF)mice(73.3%vs 20.0%,P<0.0001),and SP600125 could inhibit the effect of DHM.CONCLUSION:These findings demonstrate that DHM alleviates CCl4-induced liver injury,suggesting that DHM is a promising candidate for reversing liver injury and ALF. | Jun Xie Jie Liu Tu-Ming Chen Qing Lan Qing-Yu Zhang Bin Liu Dong Dai Wei-Dong Zhang Li-Ping Hu Run-Zhi Zhu | 2015 | World Journal of Gastroenterology2015，21，18： | 17 |
| 20 | Broadband achromatic metalens in terahertz regime显示文摘Achromatic focusing is essential for broadband operation, which has recently been realised from visible to infrared wavelengths using a metasurface. Similarly, multi-terahertz functional devices can be encoded in a desired metasurface phase profile. However, metalenses suffer from larger chromatic aberrations because of the intrinsic dispersion of each unit element. Here, we propose an achromatic metalens with C-shaped unit elements working from 0.3 to 0.8 THz with a bandwidth of approximately 91% over the centre frequency. The designed metalens possesses a high working efficiency of more than 68% at the peak and a relatively high numerical aperture of 0.385. We further demonstrate the robustness of our Cshaped metalens, considering lateral shape deformations and deviations in the etching depth. Our metalens design opens an avenue for future applications of terahertz meta-devices in spectroscopy, time-offlight tomography and hyperspectral imaging systems. | Qingqing Cheng Meilin Ma Dong Yu Zhixiong Shen Jingya Xie Juncheng Wang Nianxi Xu Hanming Guo Wei Hu Shuming Wang Tao Li Songlin Zhuang | 2019 | Science Bulletin2019，64，20： | 16 |
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