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| 1 | Control of Grain Size and Weight by the OsMKKK10-OsM KK4-OsMAPK6 Signaling Pathway in Rice显示文摘谷物尺寸是在庄稼决定谷物产量的关键农学的特点之一。然而,在庄稼位于谷物尺寸控制下面的机制留下逃犯。这里,我们证明断然表明小径的 OsMKKK10-OsMKK4-OsMAPK6 在米饭调整谷物尺寸和重量。在米饭,当组成地活跃的 OsMKKK10 (CA-OsMKKK10 ) 的 overexpression 导致大、重的谷物,长圆锥花序,和高植物时, OsMKKK10 功能的损失导致小、轻的谷物,短圆锥花序,和半矮子植物。OsMKKK10 交往与并且 phosphorylates OsMKK4。我们识别了 OsMKK4 gain-of-function 异种(large11-1D )? 那生产大、重的谷物。OsMKK4 large11-1D 等位基因编码的 A227T 比 OsMKK4 举办更强壮的 kinase 活动。植物 overexpressing OsMKK4 (OsMKK4-DD ) 的一种组成地活跃的形式也生产大谷物。进一步生物化学、基因的分析表明 OsMKKK10, OsMKK4,和 OsMAPK6 在一条普通小径工作控制谷物尺寸。一起拿,我们的学习在米饭为谷物尺寸和重量的 OsMKKK10-OsMKK4-OsMAPK6 调停串联的控制建立一个重要基因、分子的框架。 | Ran Xu Penggen Duan Haiyue Yu Zhengkui Zhou Baolan Zhang Ruci Wang Jing Li Guozheng Zhang Shangshang Zhuang Jia Lyu Na Li Tuanyao Chai Zhixi Tian Shanguo Yao Yunhai Li | 2018 | Molecular Plant2018，11，6： | 33 |
| 2 | Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger显示文摘Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. | YIN Zhi Na WU Wen Jia SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan MA Juan Juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui | 2019 | Biomedical and Environmental Sciences2019，32，1： | 14 |
| 3 | The Performance of Whole Genome Amplification Methods and Next-Generation Sequencing for Pre-Implantation Genetic Diagnosis of Chromosomal Abnormalities显示文摘Reliable and accurate pre-implantation genetic diagnosis(PGD) of patient's embryos by next-generation sequencing(NGS) is dependent on efficient whole genome amplification(WGA) of a representative biopsy sample. However, the performance of the current state of the art WGA methods has not been evaluated for sequencing. Using low template DNA(15 pg) and single cells, we showed that the two PCR-based WGA systems Sure Plex and MALBAC are superior to the REPLI-g WGA multiple displacement amplification(MDA) system in terms of consistent and reproducible genome coverage and sequence bias across the 24 chromosomes, allowing better normalization of test to reference sequencing data. When copy number variation sequencing(CNV-Seq) was applied to single cell WGA products derived by either Sure Plex or MALBAC amplification, we showed that known disease CNVs in the range of 3e15 Mb could be reliably and accurately detected at the correct genomic positions. These findings indicate that our CNV-Seq pipeline incorporating either Sure Plex or MALBAC as the key initial WGA step is a powerful methodology for clinical PGD to identify euploid embryos in a patient's cohort for uterine transplantation. | Na Li Li Wang Hui Wang Minyue Ma Xiaohong Wang Yi Li Wenke Zhang Jianguang Zhang David S.Cram Yuanqing Yao | 2015 | Journal of Genetics and Genomics2015，42，4： | 13 |
| 4 | Endoscopic management of biliary complications after orthotopic liver transplantation显示文摘BACKGROUND: Biliary complications are a serious problem in patients after liver transplantation and often require reoperation. This study was conducted to summarize the endoscopic diagnosis and management of biliary complications after orthotopic liver transplantation (OLT). METHODS: From December 2000 to November 2003, twelve endoscopic retrograde cholangiopancreatographies(ERCPs) were performed in 7 patients after OLT at Digestive Endoscopic Center of Changhai Hospital in Shanghai, China. The therapeutic maneuvers included endoscopic sphincterotomy (EST), biliary stent placement, balloon and basket extraction, irrigation, and nasobiliary tube placement. A retrospective study was made to determine the types of biliary tract complications after OLT. The success of ERCP and therapeutic maneuvers was also evaluated. RESULTS: Biliary tract complications including biliary stricture, biliary leak, biliary sludge, and stump leak of the cyst duct were treated respectively by endoscopic sphincterotomy with sludge extraction, stricture dilation or endoscopic retrograde biliary drainage. Two of the 3 patients with proximal common bile duct stricture were successfully treated with ERCP and stent placement. Four patients with anastomotic stricture and/without bile leak were treated successfully by dilation and stent placement or endoscopic nosobiliary drainage. No severe ERCP-related complications occurred. CONCLUSIONS: ERCP is an effective and accurate approach for the diagnosis of biliary tract complications after OLT, and placement of a stent is a safe initial treatment for biliary complications after liver transplantation. | Yun-Sheng Qin, Zhao-Shen Li, Zhen-Xing Sun, Ren-Pei Wu, Na Wang and Yin-Zhen Yao Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310003, China Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 11 |
| 5 | Efficacy of chitosan and sodium alginate scaffolds for repair of spinal cord injury in rats显示文摘Spinal cord injury results in the loss of motor and sensory pathways and spontaneous regeneration of adult mammalian spinal cord neurons is limited. Chitosan and sodium alginate have good biocompatibility, biodegradability, and are suitable to assist the recovery of damaged tissues, such as skin, bone and nerve. Chitosan scaffolds, sodium alginate scaffolds and chitosan-sodium alginate scaffolds were separately transplanted into rats with spinal cord hemisection. Basso-Beattie-Bresnahan locomotor rating scale scores and electrophysiological results showed that chitosan scaffolds promoted recovery of locomotor capacity and nerve transduction of the experimental rats. Sixty days after surgery, chitosan scaffolds retained the original shape of the spinal cord. Compared with sodium alginate scaffolds-and chitosan-sodium alginate scaffolds-transplanted rats, more neurofilament-H-immunoreactive cells(regenerating nerve fibers) and less glial fibrillary acidic protein-immunoreactive cells(astrocytic scar tissue) were observed at the injury site of experimental rats in chitosan scaffold-transplanted rats. Due to the fast degradation rate of sodium alginate, sodium alginate scaffolds and composite material scaffolds did not have a supporting and bridging effect on the damaged tissue. Above all, compared with sodium alginate and composite material scaffolds, chitosan had better biocompatibility, could promote the regeneration of nerve fibers and prevent the formation of scar tissue, and as such, is more suitable to help the repair of spinal cord injury. | Zi-ang Yao Feng-jia Chen Hong-li Cui Tong Lin Na Guo Hai-ge Wu | 2018 | Neural Regeneration Research2018，13，3： | 11 |
| 6 | Performance of the Prostate Health Index in predicting prostate biopsy outcomes among men with a negative digital rectal examination and transrectal ultrasonography显示文摘[-2]proPSA (p2PSA ) 和它的衍生物, p2PSA-to-free PSA 比率(% p2PSA ) ,和前列腺健康索引(PHI ) 极大地在前列腺活体检视有或没有前列腺癌症(PCa ) 改进了在人之间的辨别。然而,很少在数字直肠的考试(DRE ) 和 transrectal ultrasonography (TRUS ) 是否定的情况中对他们的表演被知道。在有否定 DRE 和 TRUS 的中国的 261 个连续病人的一个未来的队被招募并且经历前列腺活体检视。在活体检视被用来测量各种各样的 PSA 衍生物以前,一件浆液样品收集了,包括总数前列腺特定的抗原(tPSA ) ,免费 PSA,和 p2PSA。为每个病人, free-to-total PSA 比率(% fPSA ) , PSA 密度(PSAD ) , p2PSA-to-free PSA 比率(% p2PSA ) ,和 PHI 是计算的。歧视的性能在在 91% 敏感操作典型曲线(AUC ) 和活体检视率的接收装置下面用区域被估计。在关于年龄, tPSA, % fPSA, PSAD, p2PSA, % p2PSA,和 PHI 的全部队以内的 AUC 分数分别地是 0.598, 0.751, 0.646, 0.789, 0.814, 0.808,和 0.853。PHI 是前列腺活体检视结果的最好的预言者,特别在有 10.1-20 ng ml −1 的 tPSA 的病人。与另外的标记相比,在 91% 的敏感, PHI 为决定哪个人不需要经历活体检视是最有用的,从而避免不必要的过程。PHI 的使用能由在中国与否定 DRE 和 TRUS 在人之中预言前列腺活体检视结果改进 PCa 察觉的精确性。 | Guo-Peng Yu Rong Na Ding-Wei Ye Jun Qi Fang Liu Hai-Tao Chen Yi-Shuo Wu Gui-Ming Zhang Jie-Lin Sun Yao Zhu Li-Qun Huang Shan-Cheng Ren De-Ke Jiang S Lilly Zheng Hao-WenJiang Ying-Hao Sun Qiang Ding Jianfeng Xu | 2016 | Asian Journal of Andrology2016，18，4： | 10 |
| 7 | Selenium Supplementation Alleviates Autoimmune Thyroiditis by Regulating Expression of Th1/Th2 Cytokines显示文摘Selenium (Se) is an essential trace element.Autoimmune thyroid diseases (AITD) are destructive inflammatory or anti-receptor autoimmune diseases characterized by reactivity to self-thyroid antigens.However,the effects of Se on the cytokines in AITD are still unclear.So we researched the role of Selenium (Se) and Th1/Th2 cytokine productions in the pathogenesis of autoimmune thyroid diseases (AITD).The data shown that high Se intake reduced the autoantibody titers and alleviated the | TAN Long SANG Zhong Na SHEN Jun WU Yun Tang YAO Zhai Xiao ZHANG Jin Xiang ZHAO Na ZHANG Wan Qi | 2013 | Biomedical and Environmental Sciences2013，26，11： | 10 |
| 8 | Pathophysiological mechanisms and therapeutic approaches in obstructive sleep apnea syndrome显示文摘Obstructive sleep apnea syndrome(OSAS)is a common breathing disorder in sleep in which the airways narrow or collapse during sleep,causing obstructive sleep apnea.The prevalence of OSAS continues to rise worldwide,particularly in middle-aged and elderly individuals.The mechanism of upper airway collapse is incompletely understood but is associated with several factors,including obesity,craniofacial changes,altered muscle function in the upper airway,pharyngeal neuropathy,and fluid shifts to the neck.The main characteristics of OSAS are recurrent pauses in respiration,which lead to intermittent hypoxia(IH)and hypercapnia,accompanied by blood oxygen desaturation and arousal during sleep,which sharply increases the risk of several diseases.This paper first briefly describes the epidemiology,incidence,and pathophysiological mechanisms of OSAS.Next,the alterations in relevant signaling pathways induced by IH are systematically reviewed and discussed.For example,IH can induce gut microbiota(GM)dysbiosis,impair the intestinal barrier,and alter intestinal metabolites.These mechanisms ultimately lead to secondary oxidative stress,systemic inflammation,and sympathetic activation.We then summarize the effects of IH on disease pathogenesis,including cardiocerebrovascular disorders,neurological disorders,metabolic diseases,cancer,reproductive disorders,and COVID-19.Finally,different therapeutic strategies for OSAS caused by different causes are proposed.Multidisciplinary approaches and shared decision-making are necessary for the successful treatment of OSAS in the future,but more randomized controlled trials are needed for further evaluation to define what treatments are best for specific OSAS patients. | Renjun Lv Xueying Liu Yue Zhang Na Dong Xiao Wang Yao He Hongmei Yue Qingqing Yin | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 8 |
| 9 | Distribution and characteristic of PAHs in snow of Fildes Peninsula显示文摘Polycyclic aromatic hydrocarbons (PAHs) investigation in different matrices has been reported largely,whereas reports on snow samples were limited.Snow,as the main matrix in the polar region,has an important study meaning.PAHs in snow samples were analyzed to investigate the distribution and contamination status of them in the Antarctic,as well as to provide some references for global migration of PAHs.Snow samples collected in Fildes Peninsula were enriched and separated by solid-phase membrane disks and eluted by methylene dichloride,then quantified by gas chromatography/mass spectrometry.All types of PAHs were detected except for Benzo(a)pyrene.Principal component analysis method was applied to characterize them.Three factors (Naphthalene,Fluorene and Phenanthrene) accounted for 60.57%,21.61% and 9.80%,respectively.The results showed that the major PAHs sources maybe the atmospheric transportation,and the combustion of fuel in Fildes Peninsula.The comparison of concentration and types of PAHs between accumulated snow and fresh snow showed that the main compound concentrations in accumulated snow samples were higher than those in fresh ones.The risk assessment indicated that the amount of PAHs in the snow samples would not lead to ecological risk. | Guangshui Na Chunyang Liu Zhen Wang Linke Ge Xindong Ma Ziwei Yao | 2011 | Journal of Environmental Sciences2011，23，9： | 8 |
| 10 | Functional analysis of the ABA-responsive protein family in ABA and stress signal transduction in Arabidopsis显示文摘The phytohormone abscisic acid(ABA)plays an important role in plant growth and development,for example in seed dormancy and germination,as well as in plant responses to environmental stresses,such as drought and high salinity.Previous studies have shown that ABA regulates the expression of genes with an ABA-responsive element(ABRE)and their corresponding physiological responses.Bioinformatics analysis identified a GRAM domain-containing gene family that has a multiple ABRE cis-element,which was termed the ABA-responsive protein(ABR)family.To analyze the function of the ABR family,we identified homozygous T-DNA insertion mutants and constructed abr1,2,3 double mutants and triple mutant.The abr1,abr2 and abr3single mutants showed a normal phenotype;however,the germination of seeds of the double mutants and triple mutant were insensitive to ABA,NaCl,mannitol and glucose.ABR1-GFP was distributed as a punctate structure in the cytosol and may be localized in the endomembrane system.The ABR2-GFP and ABR3-GFP proteins localized in the cytoplasm.In addition,ABR1,ABR2 and ABR3 were expressed in various tissues,and could be induced by several abiotic stresses,especially by ABA.The expressions of these genes were significantly suppressed in aba2,abi1 and abi2 null mutants.These results suggested that the ABR family may act downstream of ABI1 and ABI2 in the ABA signal transduction process in plants. | LIU LingYun LI Na YAO ChunPeng MENG SaSa SONG ChunPeng | 2013 | Chinese Science Bulletin2013，58，31： | 7 |
| 11 | Biodegradation of pendimethalin by Bacillus subtilis Y3显示文摘A bacterium strain Y3,capable of efficiently degrading pendimethalin,was isolated from activated sludge and identified as Bacillus subtilis according to its phenotypic features and 16 S rRNA phylogenetic analysis.This strain could grow on pendimethalin as a sole carbon source and degrade 99.5%of 100 mg/L pendimethalin within 2.5 days in batch liquid culture,demonstrating a greater efficiency than any other reported strains.Three metabolic products,6-aminopendimethalin,5-amino-2-methyl-3-nitroso-4-(pentan-3-ylamino) benzoic acid,and 8-amino-2-ethyl-5-(hydroxymethyl)-1,2-dihydroquinoxaline-6-carboxylic acid,were identified by HPLC-MS/MS,and a new microbial degradation pathway was proposed.A nitroreductase catalyzing nitroreduction of pendimethalin to 6-aminopendimethalin was detected in the cell lysate of strain Y3.The cofactor was nicotinamide adenine dinucleotide phosphate(NADPH) or more preferably nicotinamide adenine dinucleotide(NADH).The optimal temperature and pH for the nitroreductase were 30℃ and 7.5,respectively.Hg^(2+),Ni^(2+),Pb^(2+),Co^(2+),Mn^(2+) Cu^(2+),Ag^+,and EDTA severely inhibited the nitroreductase activity,whereas Fe^(2+),Mg^(2+),and Ca^(2+) enhanced it.This study provides an efficient pendimethalin-degrading microorganism and broadens the knowledge of the microbial degradation pathway of pendimethalin. | Haiyan Ni Li Yao Na Li Qin Cao Chen Dai Jun Zhang Qin He Jian He | 2016 | Journal of Environmental Sciences2016，28，3： | 7 |
| 12 | Urinary metabonomics study of Wu-tou-tang and its co-decoction with Pinelliae Rhizoma in adjuvant-induced arthritis rats显示文摘Wu-tou-tang was applied to treat rheumatoid arthritis,rheumatic arthritis,and joint pain for thousands of years.Aconiti Radix Cocta is the primary component of Wu-tou-tang,and Aconiti Radix and Pinelliae Rhizoma is one of the famous combination taboos in traditional Chinese medicine.A urinary metabonomics method based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) had been established to quantify the changes of the endogenous metabolites in the urine of adjuvant-induced arthritis rats treated by Wutou-tang and Wu-tou-tang and Pinelliae Rhizoma(WP) co-decoctions.There was a clear separation between Wu-tou-tang and WP co-decoction treated groups in the PCA model.16 potential biomarkers had been identified which correlated with how Pinelliae Rhizoma influenced the therapeutic effect made by Wu-tou-tang.Results showed that it could reduce the therapeutic effects or make some side effects when Pinelliae Rhizoma is added in Wu-tou-tang.This is the first time to use metabonomics to investigate the reason of combination taboo. | Yao Qi Zi-Feng Pi Shu Liu Feng-Rui Song Ying Xu Na Lin Zhi-Qiang Liu | 2015 | Chinese Chemical Letters2015，26，3： | 6 |
| 13 | A global empirical model for mapping zenith wet delays onto precipitable water vapor using GGOS Atmosphere data显示文摘The importance of water vapor in research of global climate change and weather forecast cannot be over emphasized; therefore substantial efforts have been made in exploring the optimal methods to measure water vapor. It is well-established that with a conversion factor, zenith wet delays can be mapped onto precipitable water vapor(PWV). However, the determination of the exact conversion factor depends heavily on the accurate calculation of a key variable, weighted mean temperature of the troposphere(T_m). As a critical parameter in Global Positioning System(GPS) meteorology, T_m has recently been modeled into a global grid known as GWMT. The GWMT_model only requires the location and the day of year to calculate T_m. Despite the advantages that the GWMT_model offers, anomalies still exist in oceanic areas due to low sampling resolution. In this study, we refine the GWMT_model by incorporating the global T_m grid from Global Geodetic Observing System(GGOS) and obtain an improved model, GWMT-G. The results indicate that the GWMT-G model successfully addresses the anomaly in oceanic areas in the GWMT_model and significantly improves the accuracy of T_m in other regions. | YAO YiBin XU ChaoQian ZHANG Bao CAO Na | 2015 | Science China Earth Sciences2015，58，8： | 6 |
| 14 | Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued'A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)';now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients. | Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong Yue Xiao-Chun Zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang Zhou Ya-Dong Gao Zhui Yu Feng Xu Ming-Li Tu Li-Ming Tan Min Yang Fang Chen Xiao-Ju Zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang Zhang Wei Li Wen Chen Yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong Ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang | 2020 | Military Medical Research2020，7，3： | 6 |
| 15 | Ti-Zr-Be-Fe quaternary bulk metallic glasses designed by Fe alloying显示文摘A series of Ti-Zr-Be-Fe bulk metallic glasses(BMGs)with good glass-forming ability(GFA)and high specific strength have been developed.With different alloying routes and content of Fe,it is found that these alloys exhibit different GFA and mechanical properties.The effects of Fe addition on the GFA and mechanical properties of Ti-Zr-Be alloy are systemically investigated.The possible mechanisms for the improvement or damage to the GFA by addition of Fe can be interpreted in view of the mixing enthalpy,atomic size differences and electronegativity differences of the alloys,while the mechanical properties strongly depend on the Poisson’s ratio and free volume concentration.The experimental results also show that alloying technology is an effective method to improve the GFA and mechanical properties of Ti-Zr-Be glassy alloy. | GONG Pan WANG Xin SHAO Yang CHEN Na YAO KeFu | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，11： | 6 |
| 16 | High performance hydroxyapatite ceramics and a triply periodic minimum surface structure fabricated by digital light processing 3D printing显示文摘High performance hydroxyapatite(HA)ceramics with excellent densification and mechanical properties were successfully fabricated by digital light processing(DLP)three-dimensional(3D)printing technology.It was found that the sintering atmosphere of wet C02 can dramatically improve the densification process and thus lead to better mechanical properties.HA ceramics with a relative density of 97.12%and a three-point bending strength of 92.4 MPa can be achieved at a sintering temperature of 1300℃,which makes a solid foundation for application in bone engineering.Furthermore,a relatively high compressive strength of 4.09 MPa can be also achieved for a DLP-printed p-cell triply periodic minimum surface(TPMS)structure with a porosity of 74%,which meets the requirement of cancellous bone substitutes.A further cell proliferation test demonstrated that the sintering atmosphere of wet CO2 led to improve cell vitality after 7 days of cell culture Moreover,with the possible benefit from the bio-inspired structure,the 3D-printed TPMS structure significantly improved the cell vitality,which is crucial for early osteogenesis and osteointegration. | Yongxia YAO Wei QIN Bohang XING Na SHA Ting JIAO Zhe ZHAO | 2021 | Journal of Advanced Ceramics2021，10，1： | 5 |
| 17 | β-Arrestin 2 mediates arginine vasopressin-induced IL-6 induction via the ERK1/2-NF-κB signal pathway in murine hearts显示文摘Evidence to date suggests thatβ-arrestins act beyond their role as adapter proteins.Arginine vasopressin(AVP)may be a factor in inflammation and fibrosis in the pathogenesis of heart failure.In the present study we investigated the effect of AVP on inflammatory cytokine IL-6 production in murine hearts and the impact ofβ-arrestin 2-dependent signaling on AVP-induced IL-6 production.We found that administration of AVP(0.5 U/kg,iv)markedly increased the levels of IL-6 mRNA in rat hearts with the maximum level occurred at 6 h.Inβ-arrestin 2 KO mouse hearts,deletion ofβ-arrestin 2 decreased AVP-induced IL-6 mRNA expression.We then performed in vitro experiments in adult rat cardiac fibroblasts(ARCFs).We found that AVP(10?9–10?6 M)dose-dependently increased the expression of IL-6 mRNA and protein,activation of NF-κB signaling and ERK1/2 phosphorylation,whereas knockdown ofβ-arrestin 2 blocked AVP-induced IL-6 increase,NF-κB activation and ERK1/2 phosphorylation.Pharmacological blockade of ERK1/2 using PD98059 diminished AVP-induced NF-κB activation and IL-6 production.The selective V1A receptor antagonist SR49059 effectively blocked AVP-induced NF-κB phosphorylation and activation as well as IL-6 expression in ARCFs.In AVP-treated mice,pre-injection of SR49059(2 mg/kg,iv)abolished AVP-induced NF-κB activation and IL-6 production in hearts.The above results suggest that AVP induces IL-6 induction in murine hearts via the V1A receptor-mediatedβ-arrestin2/ERK1/2/NF-κB pathway,thus reveal a novel mechanism of myocardial inflammation in heart failure involving the V1A/β-arrestin 2/ERK1/2/NF-κB signaling pathway. | Shu-zhen Sun Hong Cao Na Yao Ling-ling Zhao Xiao-fang Zhu Er-an Ni Qi Zhu Wei-zhong Zhu | 2020 | Acta Pharmacologica Sinica2020，41，2： | 5 |
| 18 | The “Traditional Chinese medicine regulating liver regeneration” treatment plan for reducing mortality of patients with hepatitis B-related liver failure based on real-world clinical data显示文摘On the basis of real-world clinical data,the study aimed to explore the effect and mechanisms of the treatment plan of“traditional Chinese medicine(TCM)regulating liver regeneration.”A total of 457 patients with HBV-related liver failure were retrospectively collected.The patients were divided into three groups:the modern medicine control group(MMC group),patients treated with routine medical treatment;the control group combining traditional Chinese and Western medicine(CTW),patients treated with routine medical treatment plus the common TCM formula;and the treatment group of“TCM regulating liver regeneration”(RLR),patients treated with both routine medical treatment and the special TCM formula of RLR.After 8 weeks of treatment,the mortality of patients in the RLR group(12.31%)was significantly lower than those in the MMC(50%)and CTW(29.11%)groups.Total bilirubin level significantly decreased and albumin increased in the RLR group when compared with the MMC and CTW groups(P<0.05).In addition,there were significant differences in the expression of several cytokines related to liver regeneration in the RLR group compared with the MMC group.RLR treatment can decrease jaundice,improve liver function,and significantly reduce the mortality in patients with HBV-related liver failure.The mechanism may be related to the role of RLR treatment in influencing cytokines related to liver regeneration. | Ling Dai Xiang Gao Zhihua Ye Hanmin Li Xin Yao Dingbo Lu Na Wu | 2021 | Frontiers of Medicine2021，15，3： | 4 |
| 19 | Alleviation of acute pancreatitis-associated lung injury by inhibiting the p38 mitogen-activated protein kinase pathway in pulmonary microvascular endothelial cells显示文摘BACKGROUND Previous reports have suggested that the p38 mitogen-activated protein kinase signaling pathway is involved in the development of severe acute pancreatitis(SAP)-related acute lung injury(ALI).Inhibition of p38 by SB203580 blocked the inflammatory responses in SAP-ALI.However,the precise mechanism associated with p38 is unclear,particularly in pulmonary microvascular endothelial cell(PMVEC)injury.AIM To determine its role in the tumor necrosis factor-alpha(TNF-α)-induced inflammation and apoptosis of PMVECs in vitro.We then conducted in vivo experiments to confirm the effect of SB203580-mediated p38 inhibition on SAP-ALI.METHODS In vitro,PMVEC were transfected with mitogen-activated protein kinase kinase 6(Glu),which constitutively activates p38,and then stimulated with TNF-α.Flow cytometry and western blotting were performed to detect the cell apoptosis and inflammatory cytokine levels,respectively.In vivo,SAP-ALI was induced by 5%sodium taurocholate and three different doses of SB203580(2.5,5.0 or 10.0 mg/kg)were intraperitoneally injected prior to SAP induction.SAP-ALI was assessed by performing pulmonary histopathology assays,measuring myeloperoxidase activity,conducting arterial blood gas analyses and measuring TNF-α,interleukin(IL)-1βand IL-6 levels.Lung microvascular permeability was measured by determining bronchoalveolar lavage fluid protein concentration,Evans blue pulmonary cells was confirmed by performing a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis and examining the Bcl2,Bax,Bim and cle-caspase3 levels.The proteins levels of P-p38,NFκB,IκB,P-signal transducer and activator of transcription-3,nuclear factor erythroid 2-related factor 2,HO-1 and Myd88 were detected in the lungs to further evaluate the potential mechanism underlying the protective effect of SB203580.RESULTS In vitro,mitogen-activated protein kinase(Glu)transfection resulted in higher apoptotic rates and cytokine(IL-1βand IL-6)levels in TNF-α-treated PMVECs.In vivo,SB2035080 attenuated lung histopathological injury,decreased inflammatory activity(TNF-α,IL-1β,IL-6 and myeloperoxidase)and preserved pulmonary function.Furthermore,SB203580 significantly reversed changes in the bronchoalveolar lavage fluid protein concentration,Evans blue accumulation,terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cell numbers,apoptosis-related proteins(cle-caspase3,Bim and Bax)and endothelial microstructure.Moreover,SB203580 significantly reduced the pulmonary P-p38,NFκB,P-signal transducer and activator of transcription-3 and Myd88 levels but increased the IκB and HO-1 levels.CONCLUSION p38 inhibition may protect against SAP-ALI by alleviating inflammation and the apoptotic death of PMVECs. | Xiao-Xin Zhang Hao-Yang Wang Xue-Fei Yang Zi-Qi Lin Na Shi Chan-Juan Chen Lin-Bo Yao Xin-Min Yang Jia Guo Qing Xia Ping Xue | 2021 | World Journal of Gastroenterology2021，27，18： | 4 |
| 20 | 铜离子改性的凹凸棒石粘土在预防和治疗由沙门氏菌引起的腹泻中的应用(英文)显示文摘目的:研究开发一种抗生素替代品,应用于动物细菌性腹泻病的治疗。创新点:将凹凸棒石的吸附作用、肠道粘膜保护作用和铜离子的杀菌作用巧妙的结合在一起,能够替代抗生素,在动物细菌性腹泻的预防和治疗中发挥作用。方法:用硝酸铜和凹凸棒石粘土(Pal)制备铜离子改性的凹凸棒石粘土(CM-Pal)。通过比较添加CM-Pal前后沙门氏菌数量的变化检测抗菌活性。体内试验,将70只小鼠分为7组,其中5组感染沙门氏菌后分别给予Pal、CM-Pal、蒙脱石散、庆大霉素和生理盐水;1组在感染沙门氏菌之前给予CM-Pal,作为预防组;1组不感染沙门氏菌,作为未感染组。通过粪便形态、粪便计分、生长性能和肠绒毛高度等指标判断对沙门氏菌引起的小鼠腹泻的预防和治疗效果。结论:体外抑菌试验显示,CM-Pal与沙门氏菌作用2 h后抗菌率达100%。体内试验也显示,CM-Pal组小鼠24 h后腹泻症状消失,所有小鼠恢复正常。但是其他组小鼠(Pal组、蒙脱石散组和庆大霉素组)分别在48、48和96 h后才恢复正常。CM-Pal治疗组小鼠肠绒毛的高度在感染3 d后恢复正常,但是其他组小鼠肠绒毛高度恢复正常至少需要5 d。CM-Pal预防组小鼠增重高于其他组小鼠。本研究表明,CM-Pal在预防和治疗由沙门氏菌引起的腹泻方面能够替代抗生素具有较好的效果。 | Da-wei YAO Ze-zhong YU Na LI Yu-nong HOU Jia-rong XU De-ji YANG | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，6： | 4 |
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