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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | A DTX/MATE-Type Transporter Facilitates Abscisic Acid Efflux and Modulates ABA Sensitivity and Drought Tolerance in Arabidopsis显示文摘Abscisic 酸(骆驼毛的织物) 在植物调整众多的生理、发展的过程。最近的研究识别 intracellularABA 受体,含有越过为骆驼毛的织物察觉到和反应同样关键的房间膜的骆驼毛的织物的运输。这里,我们 reportthat DTX/Multidrug 和有毒的复合挤出(配偶) 在 Arabidopsis thaliana 的家庭成员, AtDTX50,作为骆驼毛的织物流出 transporter 工作。当在 Escherichia coli 紧张和 Xenopus 卵母细胞房间 heterologously 表示了时, AtDTX50 被发现便于骆驼毛的织物流出。而且,与骆驼毛的织物预装的 dtx50 异种叶肉房间释放了更少的骆驼毛的织物与相比野类型(WT ) 。AtDTX50 基因主要在脉管的纸巾和警卫房间被表示,它的表示由 exogenousABA 是强烈起来调整的。AtDTX50::GFP 熔化蛋白质对血浆膜主要局部性。dtx50 异种植物被观察对在生长抑制的骆驼毛的织物更敏感。另外与 WT 相比, dtx50 异种植物对有更低的有气孔的传导力的干旱更容忍,与它是的功能一致在警卫房间的骆驼毛的织物 effluxcarrier。 | Haiwen Zhang Huifen Zhu Yajun Pan Yuexuan Yu Sheng Luan Legong Li | 2014 | Molecular Plant2014，7，10： | 23 |
| 3 | Liberation characteristics of coal middlings comminuted by jaw crusher and ball mill显示文摘The associated minerals make coal middlings possess a relatively high ash content.Subsequent liberation through size reduction can cause recovery increase.However,effect of comminution facilities on mineral liberation of middlings was ignored.This paper studied the liberation characteristics of middlings crushed with different kinds of fragmentation forces.Middlings of 3 mm+0.5 mm sampled from a dense medium cyclone were comminuted by a jaw crusher and a ball mill to 0.5 mm with similar size distribution respectively.The generating mechanism of fnes was also analyzed.Full densimetric analyses indicate that mineral liberation of the product crushed by the jaw crusher is better than that by the ball mill at each fraction.For sizes of 0.125 mm+0.074 mm and 0.074 mm,yields of the product with ash content 11%comminuted by jaw crusher are nearly 20%higher than that by the ball mill.Sectional micrographs observed by the scanning electron microscopy(SEM)also show the same law for these two fractions and some intergrowth particles still exist in the fraction of 0.5 mm+0.25 mm. | Xie Weining He Yaqun Zhu Xiangnan Ge Linhan Huang Yajun Wang Haifeng | 2013 | International Journal of Mining Science and Technology2013，23，5： | 12 |
| 4 | First dark matter search results from the PandaX-Ⅰ experiment显示文摘We report on the first dark-matter(DM)search results from PandaX-I,a low threshold dual-phase xenon experiment operating at the China JinPing Underground Laboratory.In the 37-kg liquid xenon target with 17.4 live-days of exposure,no DM particle candidate event was found.This result sets a stringent limit for low-mass DM particles and disfavors the interpretation of previously-reported positive experimental results.The minimum upper limit,3.7×10-44cm2,for the spin-independent isoscalar DM-particle-nucleon scattering cross section is obtained at a DM-particle mass of 49 GeV/c2at 90%confidence level. | XIAO MengJiao XIAO Xiang ZHAO Li CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HU Jie HUANG XingTao JI XiangDong JU YongLin LEI SiAo LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing STEPHENSON Scott TAN AnDi TARL Greg WANG HongWei WANG JiMin WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIE PengWei YOU YingHui ZENG XiongHui ZHANG Hua ZHANG Tao ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，11： | 10 |
| 5 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 6 | Sini powder ameliorates the inflammatory response in rats with stress-induced non-alcoholic fatty liver disease by inhibiting the nuclear factor kappa-B/pyrin domain-containing protein 3 pathway显示文摘OBJECTIVE: To evaluate the effectiveness of Sini powder for the treatment of non-alcoholic fatty liver disease(NAFLD) in rats and the molecular mechanisms involved.METHODS: A rat model of stress-induced NAFLD was established by a combination of long-term tethering and feeding of a high-fat, high-calorie diet. These rats were then intragastrically administered with either simvastatin, Sini powder, or vehicle for 1 week. The body mass and field test scores for each group were recorded weekly. Serum aspartate aminotransferase and alanine aminotransferase activities, and triglyceride, total cholesterol,and free fatty acid concentrations were measured.Liver tissue histopathology was examined on hematoxylin and eosin-stained paraffin sections and oil red O-stained frozen sections. The hepatic m RNA expression of nuclear factor kappa-B(NF-κB),NOD-, LRR-, and pyrin domain-containing protein 3(NLRP3), apoptosis-associated speck-like protein containing CARD(ASC), and caspase-1 were measured by reverse transcription-polymerase chain reaction(RT-PCR). The hepatic protein concentrations of NF-κB and NLRP3, ASC, caspase-1, interleukin-1β(IL-1β), interleukin-6(IL-6), and the serum concentrations of IL-1β and IL-6 were measured by enzyme-linked immunosorbent assay.RESULTS: Compared with the Blank group, rats in the Compound model group showed significant pathologic manifestations of NAFLD, and the expression of NF-κB, NLRP3, ASC, caspase-1, IL-1βand IL-6 were significantly higher(all P < 0.01). Both simvastatin and Sini powder significantly ameliorated the NAFLD pathology and the abnormal expression of NF-κB, NLRP3, ASC, caspase-1, IL-1β, and IL-6(all P < 0.01).CONCLUSION: Sini powder inhibits the inflamma-tory response in rats with NAFLD, which is mediated by NF-κB/NLRP3, IL-1β, and IL-6, reduces the effects of psychological stress, and improves lipid metabolism. Therefore, Sini powder may be effective for the treatment of stress-related NAFLD through multiple mechanisms. | Mu Jie Cheng Fafeng Wang Qingguo Wang Xueqian Zhu Wenxiang Ma Chongyang Yin Xiangjun Ren Beida Lian Yajun Du Xin Zhang Haixia Liu Shuling Zhang Shuang | 2020 | Journal of Traditional Chinese Medicine2020，40，2： | 9 |
| 7 | Enhanced Power Allocation Scheme in Ultra-Dense Small Cell Network显示文摘A feasible design of ultra-dense Small cell network involves an effective solution to the interference coordination especially in cell edge. In this paper, we propose a channel state and interference aware power allocation scheme(PAG) as an enhanced solution to improve the system performance, especially for the cell-edge users. Cournot model in Non-cooperative game is employed for power adjustment in Small cell clusters to increase cell-edge users' throughput by considering the power limitation and interference coordination. Additionally, we take iterative Water Filling scheme as a comparison to clarify that the PAG scheme has struck a favorable balance between system efficiency and fairness. Simulation results show that the proposed scheme contributes to the enhancement of edge users' throughput and cells' coverage. Moreover, the scheme is observed to show a stronger adaptability in denser Small cell networks. | GAO Yuehong CHENG Lei ZHANG Xin ZHU Yajun ZHANG Yunfei | 2016 | China Communications2016，13，2： | 8 |
| 8 | Arabidopsis CNGC14 Mediates Calcium Influx Required for Tip Growth in Root Hairs显示文摘 | Sisi Zhang Yajun Pan Wang Tian Mengqi Dong Huifen Zhu Sheng Luan Legong Li | 2017 | Molecular Plant2017，10，7： | 8 |
| 9 | First experiment of spectrometric observation of hydroxyl emission and rotational temperature in the mesopause in China显示文摘A SpectroMeter of Atmospheric RadiaTion (SMART) was developed and installed at the Xinglong station of the National Astronomical Observatories in Hebei province, China, which was supported by the Meridian Project [1]. The experimental tests of spectrometric observation of the hydroxyl emission and rotational temperature in China were conducted for the first time on the night of February 23, 2011 and the night of April 27, 2011, respectively. OH 6-2 band and OH 8-3 band spectra were measured and the rotational temperature was retrieved. Hourly average temperatures (186.82±6.40) K of OH 8-3 band and (178.07±6.73) K of OH 6-2 band were derived from the spectra observed on the night of February 23, 2011. Intensities and rotational temperature against local time were determined by the spectra measured in the whole night of April 27, 2011. The rotational temperature was consistent with the spatial average temperature of NRLMSISE00 empirical model at height 83-91 km and the average temperature of TIMED/SABER from April to May of seven years at height 83-91 km, with some discrepancies. The results showed that the new instrument and the retrieval method of the rotational temperature can give reasonable results of the airglow emission of OH and the temperature of mesopause. | ZHU YaJun XU JiYao YUAN Wei LIU Xiao | 2012 | Science China(Technological Sciences)2012，55，5： | 7 |
| 10 | Geochemical characteristics of noble gases in natural gas and their application in tracing natural gas migration in the middle part of the western Sichuan Depression, China显示文摘Noble gases in natural gas, from Xiaoquan, Xinchang, Hexingchang and Fenggu gas reservoirs in the middle part of the western Sichuan Depression, China, were analysed. Results show that the volume content of crustal noble gases accounts for 97.9% to 99.7% of the total noble gas content, indicating that the noble gases in the study area are very largely derived from the crust. Moreover, the 40Ar time-accumulating effect of source rocks is used to determine the complex relationship between gases and source rocks in this area, and the results agree well with that from analysis of source rock light hydrocarbons. Due to the short migration distance, the separation of 4He and 40Ar is not significant in Xujiahe natural gas and Lower and Middle Jurassic natural gas, so it is difficult to trace natural gas migration. However, this separation characteristic of 4He and 40Ar in Middle and Upper Jurassic natural gas is significant, which indicates that natural gas migration was from the Middle Jurassic to Upper Jurassic formations. In addition, the variation trends of 3He/4He ratio and δ13 C1 value indicates that natural gas migration is from the Xujiahe formation to the Jurassic layer in the study area. | Wang Peng Shen Zhongmin Liu Sibing Lv Zhengxiang Zhu Tong Gong Yajun | 2013 | Petroleum Science2013，10，3： | 7 |
| 11 | Nutrient fluxes induced by disturbance in Meiliang Bay of Lake Taihu显示文摘A wave flume experiment was conducted to study nutrient fluxes at water-sediment interface of Meiliang Bay under different hydrodynamic conditions. The results reveal that hydrodynamics has remarkable effects on nutrient fluxes in this area. With a bottom wave stress of 0.019 N m-2 (equivalent to disturbance caused by wind SE 5-7 m s-1 at the sediment sample site of Meiliang Bay), the fluxes of TN, TDN and NH4+-N were separately 1.92× 10-3, -1.81 × 10-4 and 5.28× 10-4 mg m-2 s-1(positive for upward and negative for downward), but for TP, TDP and SRP, the fluxes were 5.69 × 10-4, 1.68 × 10-4 and -1.29 × 10-4 mgm-2 s-1. In order to calculate the released amount of nutrients based on these results, statistic analysis on the long-term meteorological data was conducted.The result shows that the maximum lasting time for wind SE 5-7 m s-1 in this area is about 15 h in summer. Further calculation shows that 111 t TN, 32 t NH4+-N, 34 t TP and 10 t TDP can be released into water (the sediment area was 47.45% of the whole surface area), resulting in concentration increase of 0.025, 0.007, 0.007 and 0.002 mg L-1 separately. With stronger disturbance (bottom wave stress is 0.217 N m-2 which is equivalent to disturbance caused by wind SE 10-11 m s-1 at the same site), there has been significant increase of nutrient fluxes (1.16× 10-2, 6.76×10-3, 1.14× 10-2 and 2.14× 10-3 mgm-2 s-1 for TN, DTN and NH4+-N and TP). The exceptions were TDP with flux having a decrease (measured to be 9.54× 10-5 mgm-2 s-1 ) and SRP with flux having a small increase (measured to be 5.42 × 10-5 mgm-2 s-1). The same statistic analysis on meteorological data reveal that the maximum lasting time for wind SE 10-11 m s-1 is no more than 5 h. Based on the nutrient fluxes and the wind lasting-time, similar calculations were also made suggesting that 232 t TN, 134.9 t TDN, 228 t NH4+-N, 42.7 t TP, 2.0 t TDP and 1.1 t SRP will be released from sediment at this hydrodynamic condition resulting in the concentration increases of 0.050, 0.029, 0.049, 0.009, 0.0004 and 0.0002 mg L-1. Therefore in shallow lakes, surface disturbance can lead to significant increase of nutrient concentrations although some components in water column had negative flux with weak disturbance (e.g. TDN and SRP in this experiment). In this case, sediment looks to be a source of nutrients. These nutrients deposited in sediment can be carried or released into water with sediment resuspension or changes of environmental conditions at water-sediment interface, which can have great effects on aquatic ecosystem and is also the characteristics of shallow lakes. | LUO Liancong, QIN Boqiang, ZHU Guangwei, SUN Xiaojing, HONG Dalin, GAO Yajun & XIE Rui Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 210008, China Nanjing Hydraulic Research Institute, Ministry of Water Resources, Nanjing 210024, China | 2006 | Science China Earth Sciences2006，49，z1： | 7 |
| 12 | The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging:An in vivo study显示文摘The application of medical devices to repair skin damage is clinically accepted and natural polymer enjoys an important role in this field,such as collagen or hyaluronic acid,etc.However,the biosafety and efficacy of these implants are still challenged.In this study,a skin damage animal model was prepared by UV-photoaging and recombinant humanized type Ⅲ collagen(rhCol Ⅲ)was applied as a bioactive material to implant in vivo to study its biological effect,comparing with saline and uncrosslinked hyaluronic acid(HA).Animal skin conditions were non-invasively and dynamically monitored during the 8 weeks experiment.Histological observation,specific gene expression and other molecular biological methods were applied by the end of the animal experiment.The results indicated that rhCol Ⅲ could alleviate the skin photoaging caused by UV radiation,including reduce the thickening of epidermis and dermis,increase the secretion of Collagen Ⅰ(Col Ⅰ)and Collagen Ⅲ(Col Ⅲ)and remodel of extracellular matrix(ECM).Although the cell-material interaction and mechanism need more investigation,the effect of rhCol Ⅲ on damaged skin was discussed from influence on cells,reconstruction of ECM,and stimulus of small biological molecules based on current results.In conclusion,our findings provided rigorous biosafety information of rhCol Ⅲ and approved its potential in skin repair and regeneration.Although enormous efforts still need to be made to achieve successful translation from bench to clinic,the recombinant humanized collagen showed superiorities from both safety and efficacy aspects. | Jing Wang He Qiu Yang Xu Yongli Gao Peijie Tan Rui Zhao Zhanhong Liu Yajun Tang Xiangdong Zhu Chongyun Bao Hang Wang Hai Lin Xingdong Zhang | 2022 | Bioactive Materials2022，7，5： | 7 |
| 13 | Creating a novel herbicide-tolerance OsALS allele using CRISPR/Cas9-mediated gene editing显示文摘Weeds and weedy rice plague commercial rice fields in many countries. Developingherbicide-tolerance rice is the most efficient strategy to control weed proliferation. CRISPR/Cas9-mediated gene editing, which generates small InDels and nucleotide substitutions atand around target sites using error-prone non-homologous end joining DNA repairing, hasbeen widely adopted for generation of novel crop germplasm with a wide range of geneticvariation in important agronomic traits. We created a novel herbicide-tolerance allele inrice by targeting the acetolactate synthase (OsALS) gene using CRISPR/Cas9-mediated geneediting. The novel allele (G628W) arose from a G-to-T transversion at position 1882 of OsALSand conferred a high level of herbicide tolerance. Transgene-free progeny carryinghomozygous G628W allele were identified and showed agronomic performance similar tothat of wild-type plants, suggesting that the G628W allele is a valuable resource fordeveloping elite rice varieties with strong herbicide tolerance. To promote use of the G628Wallele and to accelerate introgression and/or pyramiding of the G628W allele with other elitealleles, we developed a DNA marker for the G628W allele that accurately and robustlydistinguished homozygous from heterozygous segregants. Our result further demonstratesthe feasibility of CRISPR/Cas9-mediated gene editing in creating novel genetic variation forcrop breeding. | Fangquan Wang Yang Xu Wenqi Li Zhihui Chen Jun Wang Fangjun Fan Yajun Tao Yanjie Jiang Qian-Hao Zhu Jie Yang | 2021 | The Crop Journal2021，9，2： | 6 |
| 14 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 15 | Long-Term Impact of Soil Management on Microbial Biomass C, N and P in Rice-Based Cropping System显示文摘A 12-year field experiment was conducted to investigate the effect of different tillage methods and fertil- ization systems on microbial biomass C, N and P of a gray fluvo-aguic soil in rice-based cropping system. Five fertilization treatments were designed under conventional tillage (CT) or no tillage (NT) system: no fertilizer (CK); chemical fertilizer only (CF); combining chemical fertilizer with pig manure (PM); combining chemical fertilizer with crop straw (CS) and fallow (F). The results showed that biomass C, N and P were enriched in the surface layer of no-tilled soil, whereas they distributed relatively evenly in the tilled soil, which might result from enrichment of crop residue, organic manure and mineral fertilizer, and surficial development of root systems under NT. Under the cultivation system, NT had slightly greater biomass C, N and P at 0~5 cm depth, significantly less biomass C, N and P at 5~15 cm depth, less microbial biomass C, N and equivalent biomass P at 15 ~30 cm depth as compared to CT, indicating that tillage was beneficial for the multiplica tion of organisms in the plowed layer of soil. Under the fallow system, biomass C, N and P in the surface layer were significantly greater for NT than CT while their differences between the two tillage methods were negligible in the deeper layers. In the surface layer, biomass C, N and P in the soils amended with organic manure combined with mineral fertilizers were significantly greater than those of the treatments only with mineral fertilizers and the control. Soils without fertilizer had the least biomass nutrient contents among the five fertilization treatments. Obviously, the long-term application of organic manure could maintain the higher activity of microorganisms in soils. The amounts of biomass C, N and P in the fallowed soils varied with the tillage methods; they were much greater under NT than under CT, especially in the surface layer, suggesting that the frequent plowing could decrease the content of organic matter in the surface layer of the fallowed soil. | GAO YAJUN, HUANG DONGMAI, ZHU PEILI, WANG ZHIMING and LI SHENGXIU Soil and Fertilizer Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014 (China) College of Resources and Environment, Northwestern Science and Technology University of Agric | 2001 | Pedosphere2001，11，4： | 4 |
| 16 | Intron-targeted gene insertion in rice using CRISPR/Cas9: A case study of the Pi-ta gene显示文摘Intron-targeted gene insertion strategy using CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been shown to be a potential tool for crop genetic improvement by targeted mutagenesis or gene replacement of an elite allele into widely cultivated rice varieties. The rice blast resistant protein Pi-ta, differs from its susceptible counterpart, pi-ta, by a single amino acid in exon 2. To create new materials resistant to the rice blast disease, we inserted a genomic fragment containing the exon 2 and 3′ untranslated region(3′ UTR) of Pi-ta into intron 1 of pi-ta in rice materials susceptible to rice blast using the intron-targeted insertion strategy. The gene insertion frequency was3.8%. Several novel transgene-free progeny with stably inherited homozygous insert were identified in the T_1 generation, which have been crossed to rice germplasm bearing other resistance gene(R gene) for pyramiding of R genes. This work verified the feasibility of using the genome editing technology in improvement of qualitative agronomic trait in crops. | Yang Xu Fangquan Wang Zhihui Chen Jun Wang Wen-Qi Li Fangjun Fan Yajun Tao Ling Zhao Weigong Zhong Qian-Hao Zhu Jie Yang | 2020 | The Crop Journal2020，8，3： | 3 |
| 17 | Superfast and large-depth-range sinusoidal fringe generation for multi-dimensional information sensing显示文摘Among many multi-dimensional information sensing methods such as structured-light and single-pixel imaging technologies, sinusoidal fringe generation is general and crucial. Current methods of sinusoidal fringe generation force concessions in either the speed or the depth range. To mitigate this trade-off, we have simultaneously achieved both speed breakthrough and depth range enhancement by improving both the optical projection system and binary coding algorithm based on an off-the-shelf projector. Specifically, we propose a multifocal projection system and oblique projection method, which essentially eliminates the existence of a single focal plane in the conventional axisymmetric system and utilizes its anisotropy characteristics to achieve a superior filtering effect. Furthermore, the optimal pulse width modulation technique is introduced to modulate the square binary pattern for eliminating specific harmonics. To the best of our knowledge, the proposed method, for the first time,simultaneously achieved superfast(9524 frames per second) and large-depth-range(560 mm, about three times that of the conventional method) sinusoidal fringe generation with consistently high accuracy. Experimental results demonstrate the superior performance of the proposed method in multi-dimensional information sensing such as 3D, 4D, and [x, y, z, t;s(strain)]. | SIJIE ZHU ZHOUJIE WU JING ZHANG QICAN ZHANG YAJUN WANG | 2022 | Photonics Research2022，10，11： | 3 |
| 18 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 19 | Molecular mechanism of non-alcoholic fatty liver disease induced and aggravated by chronic stress through HSL/ATGL-FFA which promotes fat mobilization显示文摘Objective:To study the effects of social stress(CS)and social stress combined with high-fat diet on fat mobilization as a candidate mechanism for the induction or aggravation of non-alcoholic fatty liver disease(NAFLD).Methods:Thirty-two male Wistar rats(250±10 g)were randomly allocated to a blank control group(BC),a high-fat diet group(HFD),a CS group,and a combined CS and high-fat diet group(CS t HFD).Rats were sacrificed and tissues were collected after 8 weeks.Liver and body mass were measured and used to calculate the liver index.Serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),and free fatty acids(FFAs)were measured.Liver sections were examined microscopically after oil red O and hematoxylin and eosin staining.The relative mRNA expression of acetyl-CoA carboxylase(ACCase),hydroxymethylglutaryl coenzyme A(HMG-CoA)reductase in liver,and hormone-sensitive lipase(HSL),and adipose triglyceride lipase(ATGL)in subcutaneous adipose tissue,were measured by real-time PCR.The liver concentrations of triglyceride,reactive oxygen species,and ACCase were measured by ELISA and HSL activity was determined using turbidimetry.Results:NAFLD developed in the CS,HFD,and CS t HFD groups,with the most severe NAFLD being in the CS t HFD group.Serum AST,ALT,and FFA,liver index,and hepatic triglyceride,FFA,and tumor necrosis factor-a were significantly higher in both the CS and CS t HFD groups.However,food intake and ACCase mRNA expression were lower.The mRNA expression of HSL and ATGL in adipose tissue was much higher,and HSL activity was higher in the CS group than in the BC group,and in the CS t HFD group than in the HFD group.Conclusion:We have successfully established two models of stress-induced NAFLD,suggesting stress can induce and aggravate NAFLD by promoting fat mobilization through upregulation of HSL and ATGL. | Jie Mu Xueqian Wang Qingguo Wang Fafeng Cheng Wenxiang Zhu Changxiang Li Chongyang Ma Changming Zhai Yajun Lian Xin Du | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，4： | 2 |
| 20 | Occurrence characteristics of branching structures in equatorial plasma bubbles:a statistical study based on all-sky imagers in China显示文摘Branching structure(BS)is a very important phenomenon in the evolution of equatorial plasma bubbles(EPBs),the mechanism of which is widely studied from observation and from simulation.However,occurrence characteristics of branching structure of equatorial plasma bubbles(BSEPBs)have not been well addressed.In this work,we used seven-years(2012-2018)of observations from two all-sky imagers to study occurrence of BSEPBs in detail.These data reveal a high incidence of BS in EPB cases;in particular,most EPBs occurring on days with geomagnetic disturbances exhibited BS.Periods when all EPBs exhibited BS increased significantly in the 2014 solar maximum.Occurrence times of BSEPBs varied with local time;most of the BSEPBs began to appear between 21:00 and 22:00 LT.During the solar maximum,some BSEPBs were observed after midnight.The data also reveal that BSEPBs are characterized primarily by two branches or three branches.Multi-branching appeared only in the solar maximum.EPB events with different coexisting branching structures increased from 2012 to 2014 and decreased from 2014 to 2018.These results strongly suggest that BSEPB occurrence is related to solar activity and geomagnetic activity,and thus provide a new perspective for future studies of EPBs as well as enriching our understanding of ionospheric irregularity. | Kun Wu JiYao Xu YaJun Zhu Wei Yuan | 2021 | Earth and Planetary Physics2021，5，5： | 2 |
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