|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of a monkey with MECP2 mutations by TALEN-based gene targeting显示文摘Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey(Macaca fascicularis) carrying MECP2 mutations using transcription activator-like effector nucleases(TALENs)-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frameshifting mutation in various tissues. The monkey carrying the MECP2 mutation failed to survive after birth, due to either the toxicity of TALENs or the critical requirement of MECP2 for neural development. The level of MeCP2 protein was essentially depleted in the monkey's brain. This study demonstrates the feasibility of introducing genetic mutations in non-human primates by site-specific gene-editing methods. | Zhen Liu Xue Zhou Ying Zhu Zhi-Fang Chen Bin Yu Yan Wang Chen-Chen Zhang Yan-Hong Nie Xiao Sang Yi-Jun Cai Yue-Fang Zhang Chen Zhang Wen-Hao Zhou Qiang Sun Zilong Qiu | 2014 | Neuroscience Bulletin2014，30，3： | 17 |
| 2 | Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism显示文摘Amycolatopsis mediterranei 被用于 rifamycin 的工业规模生产,它在 antimycobacterial 治疗起一个重要作用。作为类 Amycolatopsis 的首先定序的染色体, 236 715 底配对的包括 10 的紧张 U32 的染色体,最大的原核生物的染色体之一到目前为止曾经被定序。不同于在 streptomycetes 发现的线性拓扑学,这个染色体是圆形的,特别地类似于 Saccharopolyspora erythraea 和奴卡氏菌属 farcinica,在发展史和分类代表他们的靠近的关系。尽管在 A 预言了 9 228 编码蛋白质的基因。mediterranei 染色体 S 与那些分享了 orthologs 的最大的数字。erythraea,它被 Streptomyces coelicolor 而非 N 出人意料地跟随。farcinica,显示不同新陈代谢的特征经由改编演变到多样的生态的壁龛。除类似于那的一个核心区域以外在 streptomycetes 普通,有典型核心特征的一个新奇“伪核心”在非核心区域以内被定义,在 21 从 26 基因为第二等的代谢物生产聚类的总数被定位的地方。位于核心的 rifamycin 生合成基因簇为 rifamycin SV 的变换编码细胞色素 P450 酶必需品到 B,由比作 rifamycin 生产 B 紧张 S699 的高度相应的簇揭示了并且进一步由基因互补证实了。A 的 genomic 信息。mediterranei 表明在看起来复杂的规章的机制的控制下面不仅为各种各样的碳来源和无机的氮混合物的广泛的利用而且为新陈代谢的中介的有效 funneling 安排进第二等的抗菌素合成进程的一个新陈代谢的网络。 | Wei Zhao Yi Zhong Hua Yuan Jin Wang Huajun Zheng Ying Wang Xufeng Cen Feng Xu Jie Bai Xiaobiao Han Gang Lu Yongqiang Zhu Zhihui Shao Han Yan Chen Li Nanqiu Peng Zilong Zhang Yunyi Zhang Wei Lin Yun Fan Zhongjun Qin Yongfei Hu Baoli Zhu Shengyue Wang Xiaoming Ding Guo-Ping Zbao | 2010 | Cell Research2010，20，10： | 10 |
| 3 | Study on the structure characterization and moisturizing effect of Tremella polysaccharide fermented from GCMCC5.39显示文摘The structure and moisture retention of Tremella polysaccharide fermented from GCMCC5.39(FTP)were evaluated.After UV,infrared spectrum analysis,HPAEC-PAD,HPSEC and 1 D NMR analysis,the composition of the purifi ed FTP was determined.Purifi ed components of fermented Tremella polysaccharide(FTPS)was made of galactose,mannose,glucose,galactosmine,glucosamine,and contain a large amount of hydroxyl,carbonyl and amino groups.FTPS wasα-neutral pyranose without uronic acid.FTPS-1 and FTPS-2 were obtained after purifi cation by DEAE-Sepharose Fast Flow Column.The molecular weights of FTPS-1 and FTPS-2 were 25722 and 177263 Da.FTPS-2 had a better ability to prevent moisture loss,and the optimal moisture retention period was 0–4 h.FTPS-2 could signifi cantly increase the moisture content of the skin epidermis and showed a dose-concentration relationship.The effect of FTPS-2 on the expression of different moisturizing genes was evaluated in a human skin keratinocyte model.The results showed that FTPS-2 has no cytotoxicity,and could signifi cantly promote AQP3,TGM1,CASP14,HYAL2,FLG gene expression level in HaCaT cells.It has the most signifi cant infl uence at HYAL2 protein expression on 50μg/mL. | Meng Yang Zilong Zhang Yan He Chengliang Li Jinmei Wang Xia Ma | 2021 | Food Science and Human Wellness2021，10，4： | 6 |
| 4 | Magnetic properties enhancement of hot-deformed NdFeB magnets by two different methods of CeNdCu diffusion显示文摘Two different ways were used to control the distribution of cerium for the enhancement of coercivity.One was by coating CeNdCu in the prepared magnet and annealing to make CeNdCu diffuse into the grain boundary to increase the coercivity,the other was by mixing CeNdCu with initial magnetic powders and then preparing the hot-deformed magnet.The SEM-EDS result indicates that cerium diffuses more easily into the main phase by the mixing way,while cerium is mainly distributed in the grain boundary via the coating way.The heat-treatment process may be one of the dominant influencing factors for the distribution of Ce,Multi-steps heat treatment in the mixing way,consisting of hot-pressing,hot-deforming and post heat process,easily introduces Ce into the main phase Nd2 Fe14B,which forms the Ce2 Fe14B shell resulting in the decrease of HA.But the coating way can ensure uniform dispersion of Ce in the grain boundary,which leads to the high coercivity. | Guibing Shi Zilong Wang Xinyuan Bai Wenlong Yan Yang Luo Dunbo Yu | 2020 | Journal of Rare Earths2020，38，12： | 3 |
| 5 | Cutting performance of micro-textured PCBN tool显示文摘To study the efect of micro-texture on the cutting performance of polyrystalline cubic boron nitide(PCBN)tools,five types of micro-textures(circular pits,eliptical grooves,transverse grooves,composite grooves,and wavy grooves)were applied to the rake surface of PCBN tools by an optical fber laser marking machine.Through a combination of three dimensional cutting simulations and experiments,the influences of micro-texture on chip-tool contact area,cutting force,chip morphology,shear angle,and surface roughness during the cuting process were analyzed.The results indicated that the chip--tool contact area and cutting force of both non-textured and micro textured tools increased with increasing cutting speed,while the shear angle decreased with increasing cutting speed.The chip-tool contact area and cutting force of the five types of micro-textured tools were smaller than those of the non textured tool The chip-tool contact area and cutting force obtained by the wavy-groove micro textured tool were the smallest.The chip radius produced by the five types of micro-textured tools was smaller than that produced by the non-textured tool,and the chip morphology was more stable.The transverse-groove micro-textured tool had a better chip breaking efect.The chip rnadius generated by the lliptical groove micro textured tool was 0.96 cm,while that generated by the wavy-groove tool varied from 0.55 cm to 1.26 cm.The presence of a micro-texture reduced the surface roughness of the workpiece by 11.73%-56.7%.Under the same cutting conditions,the five types of micro-textured tools gave a smaller chip--tool contact area,cutting force,chip radius,and surface roughness and a larger shear angle than the non-textured tool.In addition,the elliptical groove and wavy-groove micro-textured tools had better cuting performance. | Lin Fan Zilong Deng Xingjun Gao Yan He | 2021 | Nanotechnology and Precision Engineering2021，4，2： | 3 |
| 6 | Nlrc3-like is required for microglia maintenance in zebrafish显示文摘Microglia are tissue-resident macrophages residing in the central nervous system (CNS) and play critical roles in removing cellular debris and infectious agents as well as regulating neurogenesis and neuronal activities. Yet, the molecular basis underlying the establishment of microglia pool and the maintenance of their homeostasis in the CNS remain largely undefined. Here we report the identification and characterization of a mutant zebrafish, which harbors a point mutation in the nucleotide-binding oligomerization domain (NOD) like receptor gene nlrc3-like, resulting in the loss of microglia in a temperature sensitive manner. Temperature shift assay reveals that the late onset of nlrc3-like deficiency leads to excessive microglia cell death. Further analysis shows that the excessive microglia death in nlrc3-like deficient mutants is attributed, at least in part, to aberrant activation of canonical inflammasome pathway. Our study indicates that proper regulation of inflammasome cascade is critical for the maintenance of microglia homeostasis. | Tienan Wang Bo Yan Liang Lou Xi Lin Tao Yu Shuting Wu Qing Lu Wei Liu Zhibin Huang Mingjie Zhang Wenqing Zhang Zilong Wen | 2019 | Journal of Genetics and Genomics2019，46，6： | 2 |
| 7 | Contrasting room-temperature hydrogen sensing capabilities of Pt-SnO2 and Pt-TiO2 composite nanoceramics显示文摘察觉到行为的形成对照的房间温度氢为 Pt-TiO 2 和 Pt-SnO 2 被揭示了合成 nanoceramics。在 Pt-TiO 2 nanoceramics 的情况中,超离频当在周围的空气的氧 / 氢集中比率到达批评价值时,氢敏感突然地被失去。在 Pt-SnO 2 nanoceramics 的情况中,然而,如此的现象不发生,并且察觉到能力的非凡的房间温度氢在空中面对氧被观察。我们的联合了试验性、理论的调查为系统建立统一机制,它在表面和 SnO 2 和 TiO 2 的空隙的格子地点上根植于氢化学吸着;在 SnO 2 和 TiO 2 上的使用化学方法吸附的氢的稳定性的差别为察觉到能力的形成对照的氢被认为负责。中央调查结果在在改变混合气体的集中在房间温度充实我们和各种各样的金属氧化物半导体(苔藓) 的氢相互作用的显微镜的理解是有用的,并且他们能在基于体积苔藓开发可靠房间温度氢传感器是有帮助的。 | Yao Xiong Wanping Chen Yesheng Li Ping Cui Shishang Guo Wei Chen Zilong Tang Zijie Yan Zhenyu Zhang | 2016 | Nano Research2016，9，11： | 1 |
| 8 | Compressive sensingbased lost data recovery of fast-moving wireless sensing for structural health monitoring显示文摘 | Bao Yuequan Yan Yu Li Hui Mao Xingquan Jiao Wenfeng Zou Zilong Ou Jinping | 2014 | Structural Control and Health Monitoring2014，22，43： | 1 |
| 9 | The effect of microstructure on the laser-induced transverse voltage in Pb-doped Bi 2 Sr 2 Co 2 O y thin films on tilted substrates显示文摘 | Guoying Yan Shufang Wang Shanshan Chen Fuqiang Liu Zilong Bai Jianglong Wang Wei Yu Guangsheng Fu | 2013 | Applied Physics A2013，，4： | 1 |
| 10 | The regulation mechanism and heat transfer enhancement of composite mixed paraffin and copper foam phase change materials显示文摘Phase change materials(PCMs)have remarkable energy storage capacity and promising applications in the field of thermal control of electronic products.The problem of thermal property improvement and heat transfer of PCMs in metal-foam heatsinks is an important task for thermal management of electronic components.Mixed paraffin samples were prepared by mixing appropriate proportions of paraffin(mass)at various temperatures.Differential scanning calorimetry analysis revealed that the maximum enthalpy of 206.3 J/g is obtained by mixing 20%of 17°C liquid paraffin and 80%of 29℃ solid paraffin.Heating and cooling cycling tests revealed that mixed paraffin exhibits excellent thermal stability and that the regulation method marginally affects thermal stability.Moreover,composites were prepared by embedding PCM into a copper foam by melt impregnation.The thermal conductivity of the composites increased to 4.35 W/(m K),corresponding to 20 times its original value.In addition,density functional theory and experimental results were in good agreement,indicating that the regulation method is practical and effective. | YANG HanXue ZHANG GuanHua DOU BinLin CUI GuoMin YAN XiaoYu LU Wei WANG ZiLong | 2023 | Science China(Technological Sciences)2023，66，8： | 1 |
| 11 | 显示文摘 | Haijian Yang Zilong Li Yan Li | 2003 | Organometallics2003，22，18： | 1 |
| 12 | Single-dose AAV-based vaccine induces a high level of neutralizing antibodies against SARS-CoV-2 in rhesus macaques显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021). | Dali Tong Mei Zhang Yunru Yang Han Xia Haiyang Tong Huajun Zhang Weihong Zeng Muziying Liu Yan Wu Huan Ma Xue Hu Weiyong Liu Yuan Cai Yanfeng Yao Yichuan Yao Kunpeng Liu Shifang Shan Yajuan Li Ge Gao Weiwei Guo Yun Peng Shaohong Chen Juhong Rao Jiaxuan Zhao Juan Min Qingjun Zhu Yanmin Zheng Lianxin Liu Chao Shan Kai Zhong Zilong Qiu Tengchuan Jin Sandra Chiu Zhiming Yuan Tian Xue | 2023 | Protein & Cell2023，14，1： | 0 |
| 13 | Magnetic properties and magnetization mechanism of anisotropic NdFeB/SmFeN hybrid bonded magnets prepared with different coercivity NdFeB powders显示文摘Anisotropic NdFeB/SmFeN hybrid bonded magnets were prepared by warm compaction process under an orientation magnetic field of 22 kOe,mixing with anisotropic SmFeN powders in different addition and HDDR-NdFeB powders in different coercivity.With the addition of 20 wt% SmFeN,the density and remanence of hybrid magnets increase from 5.58 g/cm~3,8.4 kGs to 6.02 g/cm~3,9.0 kGs,respectively.And as the addition amount of SmFeN powders varies from 20 wt% to 40 wt%,the maximum energy product changes less than 0.5 MGOe.In addition,the magnetization process and the interactions between two powders were studied.It is found that the magnetization process of anisotropic NdFeB powders shows distinction in different initial states.The addition of SmFeN powders promotes the rotation of NdFeB powders together with applied field,which is beneficial to the degree of alignment of NdFeB powders.Because of the micron-sized long range coupling effect,the coercivity of hybrid magnets decreases slowly with the increase of low coercivity SmFeN.Meanwhile,the magnetization process of hybrid magnets is different from pure magnets,it increases rapidly at low field and then slowly,next leads to rapidity again and achieves the saturation magnetization finally. | Qisong Luo Yang Luo Zilong Wang Haijun Peng Wenlong Yan Wenjian Yan Tianhao Li Shengjie Zhu Dunbo Yu | 2023 | Journal of Rare Earths2023，41，9： | 0 |
| 14 | Structure and hard magnetic properties of TbCu_7-type SmFe_(8.95-x)Ga_(0.26)Nb_x nitrides显示文摘Nanocrystalline SmFe_(8.95-x)Ga_(0.26)Nb_xN_δ(x=0, 0.1, 0.2, 0.3) were prepared using rapid-quenching,annealing and nitriding. The magnetic properties and crystal structures were systematically studied under various wheel velocities to investigate the influence of Nb doping for the compounds. It is found that TbCu7-type structure is able to be obtained even though the wheel velocity is reduced to 20 m/s(x = 0.3). An significant increase(△T_c=70 ℃) of the Curie temperature is obtained with Nb doping at x = 0.1 due to the lattice expansion revealed by Rietveld analysis. The optimum coercivity with the value H_(cj) of 810 kA/m is achieved at x = 0.2 in the nitrides, in which a reasonable distribution of grain sizes of both TbCu_7-type SmFe_9 N_δ and α-Fe can be found. However, an excess of Nb doping may lead to the increase of the weight fraction of α-Fe, which in turn deteriorates the magnetic properties. | Wenlong Yan Ningtao Quan Yang Luo Dunbo Yu Zilong Wang Guiyong Wu Kun Zhang | 2018 | Journal of Rare Earths2018，36，2： | 0 |
| 15 | Phosphatidic acid-enabled MKL1 contributes to liver regeneration:Translational implication in liver failure显示文摘Liver regeneration following injury aids the restoration of liver mass and the recovery of liver function.In the present study we investigated the contribution of megakaryocytic leukemia 1(MKL1),a transcriptional modulator,to liver regeneration.We report that both MKL1 expression and its nuclear translocation correlated with hepatocyte proliferation in cell and animal models of liver regeneration and in liver failure patients.Mice with MKL1 deletion exhibited defective regenerative response in the liver.Transcriptomic analysis revealed that MKL1 interacted with E2F1 to program pro-regenerative transcription.MAPKAPK2 mediated phosphorylation primed MKL1 for its interaction with E2F1.Of interest,phospholipase d2 promoted MKL1 nuclear accumulation and liver regeneration by catalyzing production of phosphatidic acid(PA).PA administration stimulated hepatocyte proliferation and enhanced survival in a MKL1-dependent manner in a pre-clinical model of liver failure.Finally,PA levels was detected to be positively correlated with expression of pro-regenerative genes and inversely correlated with liver injury in liver failure patients.In conclusion,our data reveal a novel mechanism whereby MKL1 contributes to liver regeneration.Screening for small-molecule compounds boosting MKL1 activity may be considered as a reasonable approach to treat acute liver failure. | Jiawen Zhou Xinyue Sun Xuelian Chen Huimin Liu Xiulian Miao Yan Guo Zhiwen Fan Jie Li Yong Xu Zilong Li | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 16 | Energy-efficient removal of carbamazepine in solution by electrocoagulation-electrofenton using a novel P-rGO cathode显示文摘In this study,carbamazepine(CBZ)decay in solution has been studied by coupling electro-coagulation with electro-Fenton(EC-EF)with a novel P-rGO/carbon felt(CF)cathode,aiming to accelerate the in-situ generation of·OH,instead of adding Fe2+and H2O2.Firstly,the fabri-cated P-rGO and its derived cathode were characterized by XRD,SEM,AFM,XPS and electro-chemical test(EIS,CV and LSV).Secondly,it was confirmed that the performance in removal efficiency and electric energy consumption(EEC)by EC-EF(kobs=0.124 min^(-1),EEC=43.98 kWh/kg CBZ)was better than EF(kobs=0.069 min^(-1),EEC=61.04 kWh/kg CBZ).Then,P-rGO/CF(kobs=0.248 min^(-1),EEC=29.47 kWh/kg CBZ,CE=61.04%)showed the best performance in EC-EF,among all studied heteroatom-doped graphene/CF.This superior performance may be associated with its largest layer spacing and richest C=C,which can promote the electron transfer rate and conductivity of the cathode.Thus,more H2O2 and·OH could be produced to degrade CBZ,and almost 100%CBZ was removed with kobs being 0.337 min^(-1) and the EEC was only 24.18 kWh/kg CBZ,under the optimal conditions(P-rGO loading was 6.0 mg/cm^(2),the current density was 10.0 mA/cm^(2),the gap between electrode was 2.0 cm).Additionally,no matter the influent is acidic,neutral or alkaline,no additional pH adjustment is required for the effluent of EC-EF.At last,an inconsecutive empirical kinetic model was firstly estab-lished to predict the effect of operating parameters on CBZ removal. | Zhihui Xiao Tingyu Cui Zhenbei Wang Yan Dang Meijie Zheng Yixinfei Lin Zilong Song YipingWang Chao Liu Bingbing Xu Amir Ikhlaq Jolanta Kumirska Ewa Maria Siedlecka Fei Qi | 2022 | Journal of Environmental Sciences2022，34，5： | 0 |
| 17 | Phase-tailored assembly and encoding of dissipative soliton molecules显示文摘Self-assembly of particle-like dissipative solitons,in the presence of mutual interactions,emphasizes the vibrant concept of soliton molecules in varieties of laser resonators.Controllable manipulation of the molecular patterns,held by the degrees of freedom of internal motions,still remains challenging to explore more efficient and subtle tailoring approaches for the increasing demands.Here,we report a new phase-tailored quaternary encoding format based on the controllable internal assembly of dissipative soliton molecules.Artificial manipulation of the energy exchange of soliton-molecular elements stimulates the deterministic harnessing of the assemblies of internal dynamics.Self-assembled soliton molecules are tailored into four phase-defined regimes,thus constituting the phase-tailored quaternary encoding format.Such phase-tailored streams are endowed with great robustness and are resistant to significant timing jitter.All these results experimentally demonstrate the programmable phase tailoring and exemplify the application of the phase-tailored quaternary encoding,prospectively promoting high-capacity all-optical storage. | Yusong Liu Siyun Huang Zilong Li Haoguang Liu Yixiang Sun Ran Xia Lisong Yan Yiyang Luo Huanhuan Liu Gang Xu Qizhen Sun Xiahui Tang Perry Ping Shum | 2023 | Light(Science & Applications)2023，12，6： | 0 |
| 18 | Framework nucleic acid-based confined enzyme cascade for efficient synergistic cancer therapy in vivo显示文摘Artificial enzyme cascade systems with confinement effect are highly important in synthetic biology and biomedicine.Herein,a framework nucleic acid-based confined enzyme cascade(FNA-CEC)for synergistic cancer therapy in vivo was developed.The FNA-CEC consisted of glucose oxidase and horseradish peroxidase precisely assembled on an addressable DNA tetrahedron scaffold within few nanometers.Glucose oxidase(GOx)can trigger efficient glucose depletion for tumor starvation therapy,and increase the local concentration of H_(2)O_(2) in situ for enhanced downstream horseradish peroxidase(HRP)-activated prodrug therapy.Due to the spatial-confinement on DNA tetrahedron scaffold,the efficiency of intermediate metabolites transportation between the enzyme cascades was improved.Moreover,FNA-CEC was applied for efficient synergistic cancer therapy in vitro and in vivo.As a simple and efficient approach,the FNA-CEC is expected to expand the toolbox of technologies in synthetic biology and biomedicine. | Gezhi Kong Meng Zhang Mengyi Xiong Mei Chen Chan Yang Yan Zhao Yue Yang Zilong Zhao Zhi-Ling Song Liang Gong Guoliang Ke Xiao-Bing Zhang Weihong Tan | 2021 | Science China Chemistry2021，64，4： | 0 |
| 19 | Highly Active Rutile TiO_2 for Photocatalysis显示文摘Nanocrystalline titania was prepared by the homogeneous-precipitation method at temperature lower than 120℃. The properties of powders were identified by XRD,TEM and diffuse reflection spectra.The results showed that rutile titania formed at 70℃without calcination in air.Nanometer rutile TiO_2 had good crystalline and small particle size.The formation mechanism of rutile was also discussed.The photocatalytic activity of the prepared particles was tested for the degradation of methyl orange.The photocatalytic activity of rutile TiO_2 was higher than those obtained from Degussa P-25 TiO_2.The reasons for high photocatalytic activity of futile were discussed. | Junping Yan Zilong Tang Zhongtai Zhang Zhigang Zhou | 2006 | 稀有金属材料与工程2006，35，A03： | 0 |
| 20 | Transgenerational analysis of H3K4me3 and H3K27me3 by ChIP-Seq links epigenetic inheritance to metabolism显示文摘Histone methylation is a kind of important epigenetic modification which occurs on the lysine residue or arginine residue of histone tails(Zhang and Reinberg,2001).It takes part in multiple biological processes,including gene expression,genomic stability,stem cell maturity,genetic imprinting,mitosis and development(Fischle et al.,2005).Abnormal histone methylation pattern | Ke An Fengxia Du Hao Meng Guochao Li Minjie Zhang Zongzhi Liu Zitong Zhao Zilong Zhang Di Yu Dong Wang Caiyun Yang Wencui Ma Lin Yuan Meiting Zhou Lili Duan Li Jin Hui Li Yan Zhang Jianzhong Su Jie Qiao Yingli Sun | 2018 | Journal of Genetics and Genomics2018，45，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员