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| 1 | FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis显示文摘 | Xu Zhao Ying Yang Bao-Fa Sun Yue Shi Xin Yang Wen Xiao Ya-Juan Hao Xiao-Li Ping Yu-Sheng Chen Wen-Jia Wang Kang-Xuan Jin Xing Wang Chun-Min Huang Yu Fu Xiao-Meng Ge Shu-Hui Song Hyun Seok Jeong Hiroyuki Yanagisawa Yamei Niu Gui-Fang Jia Wei Wu Wei-Min Tong Akimitsu Okamoto Chuan He Jannie M Rendtlew Danielsen Xiu-Jie Wang Yun-Gui Yang | 2014 | Cell Research2014，24，12： | 109 |
| 2 | N^6 -methyl-adenosine (m^6 A) in RNA: An Old Modification with A Novel Epigenetic Function显示文摘N6 -methyl-adenosine (m6A) is one of the most common and abundant modifications on RNA molecules present in eukaryotes. However, the biological significance of m6A methylation remains largely unknown. Several independent lines of evidence suggest that the dynamic regulation of m6A may have a profound impact on gene expression regulation. The m6A modification is catalyzed by an unidentified methyltransferase complex containing at least one subunit methyltransferase like 3 (METTL3). m6A modification on messenger RNAs (mRNAs) mainly occurs in the exonic regions and 3'-untranslated region (3'-UTR) as revealed by high-throughput m6A-seq. One significant advance in m6A research is the recent discovery of the first two m6A RNA demethylases fat mass and obesity-associated (FTO) gene and ALKBH5, which catalyze m6A demethylation in an a-ketoglutarate (a-KG)-and Fe2+-dependent manner. Recent studies in model organisms demonstrate that METTL3, FTO and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. Moreover, perturbation of activities of these enzymes leads to the disturbed expression of thousands of genes at the cellular level, implicating a regulatory role of m6A in RNA metabolism. Given the vital roles of DNA and histone methylations in epigenetic regulation of basic life processes in mammals, the dynamic and reversible chemical m6A modification on RNA may also serve as a novel epigenetic marker of profound biological significances. | Yamei Niu Xu Zhao Yong-Sheng Wu Ming-Ming Li Xiu-Jie Wang Yun-Gui Yang | 2013 | Genomics, Proteomics & Bioinformatics2013，11，1： | 40 |
| 3 | Compound Zhebei granules combined with chemotherapy for the treatment of refractory acute leukemia: a randomized clinical trial显示文摘OBJECTIVE: To observe the effect of compound Zhebei granules(CZBG) with chemotherapy in the treatment of refractory acute leukemia.METHODS: In this multicenter, double-blind, placebo-controlled clinical trial, we used a central(online) randomization system to assign 235 patients to two treatment groups. A total of 118 patients received chemotherapy combined with CZBG(4 g,twice daily) and 117 patients received chemotherapy plus placebo. The clinical efficacy was evaluated at the end of one chemotherapeutic cycle.RESULTS: In the full analysis set, in which deaths due to disease progression were regarded as inefficacy, the rates of complete remission(CR) and partial remission(CR + PR) were 32.35% and 50.00%,respectively, for the chemotherapy combined with CZBG group, and 23.08% and 35.58%, respectively,for the chemotherapy plus placebo group. There was a statistically significant difference between the two groups according to a ysis set(PPχ2test(P < 0.05). In the per protocol analS), the CR(33.67%),CR+PR(52.04%) response rates for the chemotherapy plus CZBG group were significantly different from the response rates of the control group(CR:24.24% and CR+PR: 37.37%), respectively(P < 0.05).CONCLUSION: CZBG plus chemotherapy can improve the clinical remission rate of refractory acute leukemia after one just one therapeutic cycle. | Hou Li Yang Shulian Yang Wenhua Zhou Yuhong Liu Feng Yang Hongyong Sun Weizheng Li Dongyun Xu Yamei Chen Xinyi | 2016 | Journal of Traditional Chinese Medicine2016，36，5： | 13 |
| 4 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 5 | Cranial irradiation impairs intrinsic excitability and synaptic plasticity of hippocampal CA1 pyramidal neurons with implications for cognitive function显示文摘Radiation therapy is a standard treatment for head and neck tumors.However,patients often exhibit cognitive impairments following radiation therapy.Previous studies have revealed that hippocampal dysfunction,specifically abnormal hippocampal neurogenesis or neuroinflammation,plays a key role in radiation-induced cognitive impairment.However,the long-term effects of radiation with respect to the electrophysiological adaptation of hippocampal neurons remain poorly characterized.We found that mice exhibited cognitive impairment 3 months after undergoing 10 minutes of cranial irradiation at a dose rate of 3 Gy/min.Furthermore,we observed a remarkable reduction in spike firing and excitatory synaptic input,as well as greatly enhanced inhibitory inputs,in hippocampal CA1 pyramidal neurons.Corresponding to the electrophysiological adaptation,we found reduced expression of synaptic plasticity marker VGLUT1 and increased expression of VGAT.Furthermore,in irradiated mice,long-term potentiation in the hippocampus was weakened and GluR1 expression was inhibited.These findings suggest that radiation can impair intrinsic excitability and synaptic plasticity in hippocampal CA1 pyramidal neurons. | Min-Yi Wu Wen-Jun Zou Pei Yu Yuhua Yang Shao-Jian Li Qiang Liu Jiatian Xie Si-Qi Chen Wei-Jye Lin Yamei Tang | 2022 | Neural Regeneration Research2022，17，10： | 5 |
| 6 | Verification of Mutagen Function of Zeocin in Nannochloropsis oceanica Through Transcriptome Analysis显示文摘Zeocin can cause double strand breaks of DNA and thus is frequently used as a selective antibiotic of eukaryotic Sh ble transformants. In non-transformation system, Zeocin may function as a mutagen if not totally lethal. To verify such function of Zeocin, we mutated Nannochloropsis oceanica by increasing the concentration of Zeocin in medium gradually, and isolated a N. oceanica strain(single cell culture) which survived Zeocin up to 10.0μg mL^(-1). The Zeocin-tolerant strain entered the exponential growth phase later and grew slower than the wild strain. Transcriptome profiling showed that the Zeocin-tolerant N. oceanica strain survived Zeocin mainly by adapting(heritable), rather than acclimating(plastic) to Zeocin. Hence mutating N. oceanica with Zeocin was approved effective. Meanwhile, the physiological characteristics of this Zeocin-tolerant strain were demonstrated. As we proposed, N. oceanica tolerated Zeocin by strengthening its protein degradation and antioxidation. The genes controlling cell division and cellular response to stimuli may also have played important roles in the reduction of growth and the tolerance to Zeocin. Our findings evidenced that Zeocin can serve as an appropriate mutagen of microalgae. Creating variations through mutation with Zeocin may help to study the genetic basis of the traits of this monoploidy and asexual microalga, as well as improve its production. | LIN Genmei WANG Yamei GUO Li DING Haiyan HU Yongmei LIANG Sijie ZHANG Zhongyi YANG Guanpin | 2017 | Journal of Ocean University of China2017，16，3： | 5 |
| 7 | The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids显示文摘Magnolia biondii Pamp.(Magnoliaceae,magnoliids)is a phylogenetically,economically,and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China.Determining the genome sequence of M.biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus.We assembled a chromosome-level reference genome of M.biondii using~67,~175,and~154Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing,10X Genomics Chromium,and Hi-C scaffolding strategies,respectively.The final genome assembly was~2.22Gb,with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%.Approximately 89.17%of the genome was organized into 19 chromosomes,resulting in a scaffold N50 of 92.86Mb.The genome contained 47,547 protein-coding genes,accounting for 23.47%of the genome length,whereas 66.48%of the genome length consisted of repetitive elements.We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales.Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites,plant–pathogen interactions,and responses to stimuli,which may improve the ecological fitness and biological adaptability of the lineage.Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae,which are sister to a clade comprising monocots and eudicots.The genome sequence of M.biondii could lead to trait improvement,germplasm conservation,and evolutionary studies on the rapid radiation of early angiosperms. | Shanshan Dong Min Liu Yang Liu Fei Chen Ting Yang Lu Chen Xingtan Zhang Xing Guo Dongming Fang Linzhou Li Tian Deng Zhangxiu Yao Xiaoan Lang Yiqing Gong Ernest Wu Yaling Wang Yamei Shen Xun Gong Huan Liu Shouzhou Zhang | 2021 | Horticulture Research2021，8，1： | 4 |
| 8 | MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells显示文摘Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows,indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis.Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B(Cyclin B), cell cycle protein D(Cyclin D), cell cycle protein E(Cyclin E), and cyclin-dependent kinase 4(CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and steroidogenic acute regulatory protein(StAR)(i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively.Conclusions: Our findings suggest that miR-214-3 p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development. | Shengjie Shi Xiaoge Zhou Jingjing Li Lutong Zhang Yamei Hu Yankun Li Gongshe Yang Guiyan Chu | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 3 |
| 9 | Spectrofluorimetric analysis of captopril based on its obstruction effect of the nanomaterial surface energy transfer between acridine orange and gold nanoparticles显示文摘A simple and sensitive method for detection of captopril was established based on its obstructive effect on nanomaterial surface energy transfer(NSET). It was found that the acridine orange(AO) could be adsorbed onto the surface of citrated-gold nanoparticles(Au NPs) through electrostatic interaction. Incidentally, the fluorescence of AO was quenched owing to the dipole-dipole interaction of NSET between AO fluorophore and the Au NPs. However, captopril could obstruct the occurrence of NSET between AO and Au NPs effectively with the formation of Au-S covalent bonds between it and the Au NPs. Consequently, AO molecules were moved away from the surface of Au NPs leading to a decline of the energy transfer efficiency. Moreover, the fluorescence of AO could be gradually restored with the addition of captopril. Under the optimal conditions, the recovered fluorescence intensity correlated linearly with the concentration of captopril in the range of 400 nmol/L–2.0 μmol/L with a detection limit of 71 nmol/L. Besides, the proposed method was successfully applied for the detection of captopril in troches with the recovery of 93%–102% and the RSD lower than 2.24%. The results were in good agreement with those obtained from the HPLC method. | Jingfang Guo Yamei Yang Xiaoli Hu Yuanfang Li | 2015 | Science China Chemistry2015，58，5： | 2 |
| 10 | Spontaneous Ga whisker formation on FeGa_3显示文摘FeGa_3 has attracted considerable attention as potential material for many fields, whereas the whiskering phenomenon may cast doubts on its applications. Spontaneous Ga whisker formation was observed on the ballmilled FeGa_3-Ga. The sample made from ball-milled FeGa_3 powders free of elemental Ga are immune to the Ga whisker formation, even if all the processing parameters were the same as the FeGa_3-Ga composite sample. In addition, several nano-sized Ga whiskers were found on the pressed FeGa_3-Ga composite without ball milling,and the as-synthesized FeGa_3-Ga grew only tiny Ga balls on the surface. These whiskering phenomena do not follow the traditional metal whisker formation mechanisms. A newly proposed catalysis-based mechanism, in which the cleavage planes of FeGa_3 grains act as heterogeneous nucleation sites and the elemental Ga provides Ga atoms for whisker formation, is employed to explain Ga whisker formation on FeGa_3. | Yushuang Liu Peigen Zhang Li Yang Wubian Tian Yamei Zhang Zhengming Sun | 2018 | Progress in Natural Science:Materials International2018，28，5： | 1 |
| 11 | Mechanism and mitigation of spontaneous Ga whisker growth on Cr2GaC显示文摘Spontaneous growth of A-element whiskers on Mn+1AXn(MAX for short) phase materials poses a barrier to their practical applications, since it casts doubts on their stability. In this study, Ga whisker growth on sintered Cr2GaC samples was investigated. The elemental source for spontaneous growth of Ga whiskers is identified as the free Ga contained in the Cr2GaC material, not the lattice atoms from Cr2GaC grains, which removes the doubts on the stability of Cr2GaC material. The growth behavior and morphologies of the Ga whiskers follow a new catalysis-based model, with cleavage planes of Cr2GaC grains involved as nucleation sites. This model explains and predicts well the growth behavior of the whiskers. The mitigation strategy based on this model is in principle simple: to prevent free Ga in Cr2GaC material or limit it to a certain level;to avoid cleavage plane of Cr2GaC grains;to achieve high density of the Cr2GaC material. | ZHANG PeiGen DING JianXiang LIU YuShuang YANG Li TIAN WuBian OUYANG Jian ZHANG YaMei SUN ZhengMing | 2020 | Science China(Technological Sciences)2020，63，3： | 1 |
| 12 | The salt and soluble solids content evaluation of pickled cucumbers based on inductive electrical parameters 显示文摘 | Jin Yamei Yang Na Ma Qian | 2015 | Food and Bioprocess Technology2015，8，4： | 1 |
| 13 | A method of analysis for T-2 toxin and neosolaniol by UHPLC-MS/MS in apple fruit inoculated with Trichothecium roseum显示文摘 | TANG Yamei XUE Huali BI Yang | 2015 | Food Additives & Contaminants: Part A2015，32，4： | 1 |
| 14 | Effects of sintering temperature on properties of porous mullite/corundum ceramic显示文摘 | Yang Fengkun Li Cuiwei Lin Yamei | | 0，，： | 1 |
| 15 | Research on MRI Brain Segmentation Algorithm with the Application in Model-based EEG/MEG 显示文摘 | He Shijuan Shen Xueqin Yang Yamei | 2001 | IEEE Trans on Mag2001，37，5： | 1 |
| 16 | Interfacial fracture toughness of APS bond coat/substrate under high temperature显示文摘 | Qi Hongyu Yang Xiaoguang Wang Yamei | 2009 | International Journal of Fracture (-)2009，，1： | 1 |
| 17 | Bi nanoparticles in situ encapsulated by carbon film as high-performance anode materials for Li-ion batteries显示文摘Bismuth (Bi) has indeed inspired great interests in lithium-ion batteries (LIBs) due to the high capacity,but was still limited by the low electrical conductivity and large volume variation.Herein,a composite material based on Bi nanoparticles in situ encapsulated by carbon film (Bi@CF) is prepared successfully through a facile metal–organic framework (MOF)-engaged approach.As anode materials for LIBs,the Bi@CF composites achieved high reversible capacities of 705 and 538 mAh g^(-1)at 0.2 and 0.5 A g^(-1) after200 cycles,and long cycling performance with a stable capacity of 306 mAh g^(-1)at 1.0 A g^(-1) even after 900 cycles.In situ X-ray diffraction (XRD) measurements clearly revealed the conversion between Bi and Li_(3)Bi during the alloying/dealloying process,confirming the good electrochemical reversibility of Bi@CF for Li-storage.The reaction kinetics of this Bi@CF composite was further studied by galvanostatic intermittent titration technique (GITT).This work may provide an inspiration for the elaborate design and facile preparation of alloy-type anode materials for high-performance rechargeable batteries. | Jun Yang Jiahui Xian Qinglin Liu Yamei Sun Guangqin Li | 2022 | Journal of Energy Chemistry2022，31，6： | 1 |
| 18 | DCLK1 autoinhibition and activation in tumorigenesis显示文摘Doublecortin-like kinase 1(DCLK1)is upregulated in many tumors and is a marker for tumor stem cells.Accumulating evidence suggests DCLK1 constitutes a promising drug target for cancer therapy.However,the regulation of DCLK1 kinase activity is poorly understood,particularly the function of its autoinhibitory domain(AID),and,moreover,no physiological activators of DCLK1 have presently been reported.Here we determined the first DCLK1 kinase structure in the autoinhibited state and identified the neuronal calcium sensor HPCAL1 as an activator of DCLK1.The C-terminal AID functions to block the ATP-binding site and is competitive with ATP.HPCAL1 binds directly to the AID in a Ca^(2+)-dependent manner,which releases the autoinhibition.We also analyzed cancer-associated mutations occurring in the AID and elucidate how these mutations disrupt DCLK1 autoinhibition to elicit kinase activity upregulation.Our results present a molecular mechanism for autoinhibition and activation of DCLK1 kinase activity and provide insights into DCLK1-associated tumorigenesis. | Linna Cheng Zejing Yang Wenhao Guo Chengyong Wu Shufang Liang Aiping Tong Zhongwei Cao Rick F.Thorne Sheng-Yong Yang Yamei Yu Qiang Chen | 2022 | The Innovation2022，3，1： | 1 |
| 19 | Cumulative cellulolytic enzyme activities and initial litter quality in prediction of cellulose degradation in an alpine meadow of the eastern Tibetan Plateau显示文摘植物凋落物分解是决定陆地生态系统碳和养分循环的关键生态系统过程。作为凋落物的主要组成部分,纤维素是与凋落物分解相关的微生物的重要能量来源。纤维素酶在凋落物纤维素降解过程中的重要作用已为人们所熟知,然而纤维素降解的季节模式、累积酶活性和凋落物质量是否能预测高寒草甸的纤维素降解仍是一个未解之谜,这限制了我们对草本植物凋落物纤维素降解的认识。为了探究纤维素降解的季节性模式以及累积纤维素分解酶活性和凋落叶初始质量对纤维素降解的影响,我们在青藏高原东部的高山草甸选取了三种优势种[圆叶筋骨草(Ajuga ovalifolia)、藏羊茅(Festuca wallichanica)和草甸马先蒿(Pedicularis roylei)],进行了为期两年的凋落物网袋分解实验。我们的研究发现,纤维素在第一年中迅速降解且降解率超过50%,而且主要发生在第一个生长季(31.9%-43.3%)在两年的分解过程中,纤维素降解由累积内切葡聚糖酶(R^(2)=0.70),累积纤维二糖水解酶(R^(2)=0.59)和累积1,4-β-葡糖苷酶(R^(2)=0.57)共同驱动。此外,在这两年的分解过程中,纤维素、可溶性有机碳、总酚、木质素的浓度和木质素/N可以解释纤维素降解变异的(52%-78%)。用初始纤维素浓度模型预测纤维素降解效果最佳(R^(2)=0.78)。在凋落物的分解过程中,酶效率和微生物对纤维素分解酶的分配因物种而异。藏羊茅凋落物中纤维素酶效率较高,但质量相对较低。与草甸马先蒿相比,完全降解圆叶筋骨草和藏羊茅的纤维素需要4倍和6.7倍的内切葡聚糖酶、3倍和4.5倍的纤维二糖水解酶、1.2倍和1.4倍的1,4-β-葡糖苷酶。我们的研究结果表明,虽然微生物酶活性和凋落物初始质量都对高山草甸纤维素降解有显著影响,但使用纤维素浓度来预测纤维素降解是简化凋落物分解过程中纤维素降解和C循环模型的好方法。 | Yamei Chen Yang Liu Jian Zhang Wanqin Yang Changchun Deng Runlian He | 2020 | Journal of Plant Ecology2020，13，1： | 1 |
| 20 | Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height显示文摘Switchgrass(Panicum virgatum L.)is an important perennial,noninvasive,tall ornamental grass that adds color and texture to gardens and landscapes.Moreover,switchgrass has been considered a forage and bioenergy crop because of its vigorous growth,low-input requirements,and broad geography.Here,we identified PvWOX3a from switchgrass,which encodes a WUSCHEL-related homeobox transcription factor.Transgenic overexpression of PvWOX3a in switchgrass increased stem length,internode diameter,and leaf blade length and width,all of which contributed to a 95%average increase in dry weight biomass compared with control plants.Yeast one-hybrid and transient dual-luciferase assays showed that PvWOX3a can repress the expression of gibberellin 2-oxidase and cytokinin oxidase/dehydrogenase through apparently direct interaction with their promoter sequences.These results suggested that overexpression of PvWOX3a could increase gibberellin and cytokinin levels in transgenic switchgrass plants,which promotes cell division,elongation,and vascular bundle development.We also overexpressed PvWOX3a in a transgenic miR156-overexpressing switchgrass line that characteristically exhibited more tillers,thinner internodes,and narrower leaf blades.Double transgenic switchgrass plants displayed significant increases in internode length and diameter,leaf blade width,and plant height but retained a tiller number comparable to that of plants expressing miR156 alone.Ultimately,the double transgenic switchgrass plants produced 174%more dry-weight biomass and 162%more solubilized sugars on average than control plants.These findings indicated that PvWOX3a is a viable potential genetic target for engineering improved shoot architecture and biomass yield of horticulture,fodder,and biofuel crops. | Ruijuan Yang Zhenying Wu Chen Bai Zhichao Sun Mengqi Wang Yuzhu Huo Hailing Zhang Yamei Wang Huapeng Zhou Shaojun Dai Wenwen Liu Chunxiang Fu | 2021 | Horticulture Research2021，8，1： | 1 |
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