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| 1 | microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma显示文摘间充质的干细胞(MSC ) 被表明了为许多神经病学的疾病有有希望的治疗学的好处;然而,内在的机制糟糕被理解。这里,我们显示出 MSC 的那 intravitreal 注入在尖锐绿内障的一个老鼠模型的支持的网膜的中心细胞(RGC ) 幸存与 microglial 激活的重要抑制, TNF- , IL-1,和反应的氧种类的生产,以及 caspase-8 和 caspase-3 激活。在 vitro, MSC 禁止了两 caspase-8-mediated RGC apoptosis 和 microglial 激活,部分经由 stanniocalcin 的行动 1 (STC1 ) 。而且,我们发现了那 microRNA-21a-5p (miR-21 ) 和它的目标, PDCD4,为 STC1 生产和在 vitro 并且在 vivo 的 MSC 的 neuroprotective 性质是必要的。重要地, miR-21 overexpression 或 PDCD4 尖锐绿内障上的击倒的扩充调停 MSC 的 neuroprotective 效果。miR-21/PDCD4 轴由导致 MSC 分泌施加 neuroprotective 的 STC1 和另外的因素的以前未被认出的 neuroprotective 机制完成的这些数据热点。因此, modulating miR-21/PDCD4 轴可能是为尖锐绿内障和另外的神经病学的疾病的临床的处理的有希望的策略。 | Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng Yehong Zhuo | 2017 | Journal of Molecular Cell Biology2017，9，4： | 5 |
| 2 | The essential oil components of Cinnamomum cassia: an analysis under different thinning models of plantation Pinus massoniana显示文摘Thinning is an important activity employed in forest management. To date, studies have mainly focused on the effects of thinning on the growth of trees during the same thinning period. In this study, plantation Pinus massoniana Lamb. near maturity were thinned at varying intensities and an economically important species, Cinnamomum cassia Presl., was planted beneath the thinned canopy. The aim of the study was to explore the effects of the extent of thinning on the essential oil content and its components of C. cassia in different parts of the plant, as well as the economic feasibility of the P. massoniana–C.cassia management model. Thinning significantly reduced the oil yield in the bark and branches of C. cassia, but hardly impacted the oil yield from C. cassia leaves compared with pure C. cassia forest(CK). Among the different thinning treatments, both light(T.4) and extensive(T.1)thinning reduced the oil yield of C. cassia bark and new branches. The concentrations of the main aldehydes differed in different parts of the plant and were affected by the extent of thinning. The influence on cinnamaldehyde in the bark was minor, but was much greater in the branches and leaves. Both the oil yield and content of cinnamaldehyde showed that moderate(T.3) thinning was more favorable than other thinning models. These results not only provide a potentially promising model for the transformation of low–yield artificial pure forests of P. massoniana in the future, but also offer a reference for the management of artificial mixed stands. | Dayan Tao Yuanfa Li Daodiao Lu Yehong Luo Sufang Yu Shaoming Ye | 2016 | Journal of Forestry Research2016，27，3： | 5 |
| 3 | Rhodojaponin Ⅵ indirectly targets Cav2.2 channels via N-ethylmaleimide-sensitive fusion protein to alleviate neuropathic pain显示文摘Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed.Rhodojaponin Ⅵ,a grayanotoxin from Rhododendron molle,showed remarkable antinociceptive efficacy in models of neuropathic pain,but its biotargets and mechanisms are unknown.Given the reversible action of rhodojaponin Ⅵ and the narrow range over which its structure can be modified,we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin Ⅵ.N-Ethylmaleimide-sensitive fusion(NSF) was confirmed as the key target of rhodojaponin Ⅵ through biological and biophysical experiments.Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca2+current intensity,whereas rhodojaponin Ⅵ reversed the effects of NSF.In conclusion,rhodojaponin Ⅵ represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF. | Keliang Chen Tao Wang Yong Li Jun Wu Cheng-Xiao Zhao Sheng Liu Fengrun Sun Yehong Fang Jiahuan Hu Jinping Hu Chong-Jing Zhang Haibo Yu Chao Ma Shi-Shan Yu | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 2 |
| 4 | Aquaporin-1 Expressed in Cultured Human Trabecular Meshwork Cells显示文摘Objective:To determine if aquaporin-1 could be detected in cultures of human trabecularshwork cells. Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study the endogenous expression of aquaporin-1 and its possible role inthe regulation of aqueous outflow. | Mingkai Lin, Jian Ge, Yehong Zhuo, Yuqing Lan, Keming Yu, Jianliang ZhengZhongshan Ophthalmic center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | Eye Science2002，18，1X： | 2 |
| 5 | MKP-1 reduces Aβ generation and alleviates cognitive impairments in Alzheimer’s disease models显示文摘Mitogen-activated protein kinase(MAPK)phosphatase 1(MKP-1)is an essential negative regulator of MAPKs by dephosphorylating MAPKs at both tyrosine and threonine residues.Dysregulation of the MAPK signaling pathway has been associated with Alzheimer’s disease(AD).However,the role of MKP-1 in AD pathogenesis remains elusive.Here,we report that MKP-1 levels were decreased in the brain tissues of patients with AD and an AD mouse model.The reduction in MKP-1 gene expression appeared to be a result of transcriptional inhibition via transcription factor specificity protein 1(Sp1)cis-acting binding elements in the MKP-1 gene promoter.Amyloid-β(Aβ)-induced Sp1 activation decreased MKP-1 expression.However,upregulation of MKP-1 inhibited the expression of both Aβprecursor protein(APP)andβ-site APP-cleaving enzyme 1 by inactivating the extracellular signal-regulated kinase 1/2(ERK)/MAPK signaling pathway.Furthermore,upregulation of MKP-1 reduced Aβproduction and plaque formation and improved hippocampal long-term potentiation(LTP)and cognitive deficits in APP/PS1 transgenic mice.Our results demonstrate that MKP-1 impairment facilitates the pathogenesis of AD,whereas upregulation of MKP-1 plays a neuroprotective role to reduce Alzheimer-related phenotypes.Thus,this study suggests that MKP-1 is a novel molecule for AD treatment. | Yehong Du Yexiang Du Yun Zhang Zhilin Huang Min Fu Junjie Li Yayan Pang Peng Lei Yu Tian Wang Weihong Song Guiqiong He Zhifang Dong | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 2 |
| 6 | Interfacial studies of sisal fiber reinforced hing density polyethylene ( HDPE ) composites 显示文摘 | Li Yan Hu Chunjing Yu Yehong | 2008 | Composites ( Part A )2008，39，： | 1 |
| 7 | Interfacial studies of sisal fiber reinforced high density polyethylene (HDPE) composites 显示文摘 | Yan Li Chunjing Hu Yehong Yu | 2007 | Composites : Part A2007，39，： | 1 |
| 8 | Interfacial studies of sisal fiber reinforced high density polyethylene composites 显示文摘 | Yan Li Chunjing Hu Yehong Yu | 2008 | Composites2008，，： | 1 |
| 9 | Numerical Study on the Probability of Fatigue Failure of a Semi-Submerged Platform Structure显示文摘On the basis of Miner’s linear cumulative damage theorem, random variables are introduced to evaluate objectively the fatigue damage of a semi-submerged platform structure and a method is presented to analyze the fatigue reliability of the structure in its design life. The reliability of the method is verified through numerical examples and some conclusions are drawn, which have certain guiding and reference value for design and inspection. | Yu Jianxing, Hu Yunchang , Wang Yehong and YuHongjie Associate Professor, Tianjin University, Tianjin, 300072 Professor, Tianjin University, Tianjin, 300072 Postgraduate for Master Degree, Tianjin University, Tianjin, 300072 | 1994 | China Ocean Engineering1994，9，4： | 0 |
| 10 | Energy Spectra of Ions Sputtered from Surface by 200 keV Ar2+显示文摘 | Ruan Fangfang Wu Yehong Du Fan Yu Deyang Chen Jing Wang Wei Zhang Mingwu Shao Caojie Lu Rongchun Cai Xiaohong | 2011 | IMP & HIRFL Annual Report2011，，1： | 0 |
| 11 | Production of Slow Highly Charged Ions Microbeams with Focusing Effect through a Tapered Glass Capillary显示文摘 | Xue Yingli Chen Jing Liu Junliang Wu Yehong Ruan Fangfang Wang Wei Yu Deyang Cai Xiaohong | 2011 | IMP & HIRFL Annual Report2011，，1： | 0 |
| 12 | Angular Distribution of Lyman-1 Transition Following Nonradiative Electron Capture into Xe^53+Ions显示文摘Radiative electron capture(REC)and nonradiative capture(NRC)are two competing mechanisms in relativistic collisions of highly charged ions with atoms[1].REC means electron transfer from target to projectile with the emission of one photon for satisfying energy and momentum conservation laws. | Yang Bian Yu Deyang Cai Xiaohong Shao Caojie Xue Yingli Wang Wei Zhang Mingwu Liu Junliang Song Zhangyong Wu Yehong Lu Rongchun Ruan Fangfang | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 13 | Identification and validation of key biomarkers and potential therapeutic targets for primary open-angle glaucoma显示文摘Primary open-angle glaucoma(POAG)is a prevalent cause of blindness worldwide,resulting in degeneration of retinal ganglion cells and permanent damage to the optic nerve.However,the underlying pathogenetic mechanisms of POAG are currently indistinct,and there has been no effective nonsurgical treatment regimen.The objective of this study is to identify novel biomarkers and potential therapeutic targets for POAG.The mRNA expression microarray datasets GSE27276 and GSE138125,as well as the single-cell high-throughput RNA sequencing(sc RNA-seq)dataset GSE148371 were utilized to screen POAGrelated differentially expressed genes(DEGs).Functional enrichment analyses,protein-protein interaction(PPI)analysis,and weighted gene co-expression network analysis(WGCNA)of the DEGs were performed.Subsequently,the hub genes were validated at a single-cell level,where trabecular cells were annotated,and the mRNA expression levels of target genes in different cell clusters were analyzed.Immunofluorescence and quantitative real-time PCR(q PCR)were performed for further validation.DEGs analysis identified 43 downregulated and 32 upregulated genes in POAG,which were mainly enriched in immune-related pathways,oxidative stress,and endoplasmic reticulum(ER)stress.PPI networks showed that FN1 and DUSP1 were the central hub nodes,while GPX3 and VAV3 were screened out as hub genes through WGCNA and subsequently validated by q PCR.Finally,FN1,GPX3,and VAV3 were determined to be pivotal core genes via single-cell validation.The relevant biomarkers involved in the pathogenesis of POAG,may serve as potential therapeutic targets.Further studies are necessary to unveil the mechanisms underlying the expression variations of these genes in POAG. | Jian Wu Caixia Lin Chenlong Yang Lijie Pan Hongyi Liu Sirui Zhu Shuwen Wei Xu Jia Qi Zhang Ziyu Yu Xiaofang Zhao Weihai Liu Yehong Zhuo Ningli Wang | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 14 | Corrigendum to‘microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma’显示文摘Journal of Molecular Cell Biology(2017),9(4),289‒301,http://gffzzd3cc09b8251d45dfsp9cnnnu906up69q6.ffgz.tsg.suse.edu.cn/10.1093/jmcb/mjx022 In this article,there were errors in Figures 4D(western blotting analysis),3E and F,5G and H,and 5J and K(flow cytometry analysis).Iba1 band in Figure 4D was the same as Cleaved caspase-3 band in Figure 2F.This was caused by carelessly mistaking the latter(Cleaved caspase-3,#1)for Iba1 band due to the high similarity between images(raw blot images of these bands from three experiments are provided in the Supplementary material).The corrected Figure 4D with updated Iba1 band(#1)and quantitation graph is shown below. | Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng Yehong Zhuo | 2023 | Journal of Molecular Cell Biology2023，15，4： | 0 |
| 15 | 50-200 MeV/u全裸氙离子与氪、氙气体靶碰撞的电子俘获及辐射退激发显示文摘In collisions between energetic highly charged ions and atoms,the ions could remove the electrons of the target atoms,or be excited into high-level states.In the first case,the target electrons not only be captured into ground and excited states of the projectile ions,but also be ionized to free.Non-radiative capture(NRC)and radiative electron capture(REC)are two competing mechanisms in fast collisions[1].NRC means electron transfer from a bound state of the target to a bound states of the projectile without the emission of radiation;REC is produced with simultaneous the emission of photon for satisfying energy and momentum conservation laws.The physical essence is the competition between the“electron-nucleus”interaction and the“electron-vacuum”interaction.NRC is dominant in collisions of high-Z ions with heavy target atoms at moderate energies.REC entirely determine the electron capture channel for high collision velocities and light targets[1].Measuring the relative strength of the projectile x-ray emission associated with electron capture could determine state-selective population,angular distribution of single capture Ly 1 or double capture Kα1 and Kα2,would provide new detailed information on the dynamics of ion-atom collisions and atomic structure studies[1]. | Yang Bian Yu Deyang Cai Xiaohong Xue Yingli Shao Caojie Wang Wei Liu Junliang Zhang Mingwu Song Zhangyong Wu Yehong Lu Rongchun Ruan Fangfang | 2017 | IMP & HIRFL Annual Report2017，，1： | 0 |
| 16 | GABAergic interneurons mediate inhibitory signal from A-fiber to nocicetive C-fiber显示文摘We investigate the morphological and electrophysiological mechanism of gate control for pain.We proposed that the regulation of peripheral A-fibers on C fibers was mediated by the GABAergic interneurons in the spinal cord.The cobra venom was injected to the sciatic nerve to establish a neuropathic pain model due to peripheral demyelination within A-fiber.The content of r aminobutyric acid(GABA)in spinal cord and cerebrospinal fluid was asssed by high performance liquid chromatography(HPLC).The microstructure of A-fiber,C-fiber and the GABAergic neurons in the spinal cord was observed by immunofluorescence staining. | Yu Ning Cui Huan Fang Yehong Liu Fan Wang Tao Yuan Bo Ma Chao | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 17 | Glutamate and GABAA receptor crosstalk mediates homeostatic regulation of neuronal excitation in the mammalian brain显示文摘Maintaining a proper balance between the glutamate receptor-mediated neuronal excitation and the A type of GABA receptor(GABAAR)mediated inhibition is essential for brain functioning;and its imbalance contributes to the pathogenesis of many brain disorders including neurodegenerative diseases and mental illnesses.Here we identify a novel glutamate-GABAAR interaction mediated by a direct glutamate binding of the GABAAR.In HEK293 cells overexpressing recombinant GABAARs,glutamate and its analog ligands,while producing no current on their own,potentiate GABA-evoked currents.This potentiation is mediated by a direct binding at a novel glutamate binding pocket located at theα+/β−subunit interface of the GABAAR.Moreover,the potentiation does not require the presence of aγsubunit,and in fact,the presence ofγsubunit significantly reduces the potency of the glutamate potentiation.In addition,the glutamate-mediated allosteric potentiation occurs on native GABAARs in rat neurons maintained in culture,as evidenced by the potentiation of GABAAR-mediated inhibitory postsynaptic currents and tonic currents.Most importantly,we found that genetic impairment of this glutamate potentiation in knock-in mice resulted in phenotypes of increased neuronal excitability,including decreased thresholds to noxious stimuli and increased seizure susceptibility.These results demonstrate a novel cross-talk between excitatory transmitter glutamate and inhibitory GABAAR.Such a rapid and short feedback loop between the two principal excitatory and inhibitory neurotransmission systems may play a critical homeostatic role in fine-tuning the excitation-inhibition balance(E/I balance),thereby maintaining neuronal excitability in the mammalian brain under both physiological and pathological conditions. | Ya Wen Zhifang Dong Jun Liu Peter Axerio-Cilies Yehong Du Junjie Li Long Chen Lu Zhang Lidong Liu Jie Lu Ning Zhou Dong Chuan Wu Yu Tian Wang | 2022 | Signal Transduction and Targeted Therapy2022，7，11： | 0 |
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