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| 1 | Fluxes of CH4 and N_2O from soil under a tropical seasonal rain forest in Xishuangbanna, Southwest China显示文摘从在在 Xishuangbanna 的热带季节的雨林下面的土壤的 CH4 和 N2O 流动,西南中国用关上的静态的房间技术和气体色层法方法被测量一年。三个处理被放在学习的地里:(A) 没有崽,有垃圾的(B) ,和有垃圾和幼苗的(C) 。结果证明在我们的学习的土壤是大气的 CH4 的一个水池和大气的 N2O 的来源。从处理 A, B,和 C 的观察吝啬的 CH4 流动是 50.0 ± 4.0, 35.9 ± 2.8, 31.6 ± 2.8 μ g C/(m2 · h ) ,分别地并且在 2003 的计算年度流动分别地是 4.1, 3.1,和 2.9 kgC/hm2。从处理 A, B,和 C 的观察吝啬的 N2O 流动是 30.9 ± 3.1, 28.2 ± 3.5, 50.2 ± 3 .7 μ g N/(m2 · h ) ,分别地并且在 2003 的计算年度流动分别地是 4.1, 3.1,和 2.9 kgC/hm2。从处理 A, B,和 C 的观察吝啬的 N2O 流动是 30.9 ± 3.1, 28.2 ± 3.5, 50.2 ± 3 .7 μ g N/(m2 · h ) ,分别地并且在 2003 的计算年度流动是 2.8, 2.6,和 3.7 kgN/hm 2 分别地。在 CH4 和 N2O 流动的季节变化在所有三个处理之中是重要的。垃圾的存在减少了 CH4 举起在湿季节期间(P < 0.05 ) ,然而并非在干旱期期间。调停幼苗的 N2O 排出物有类似的增加在期间湿并且干旱期,显示幼苗在两个季节增加了 N2O 排放。一种强壮的积极关系为处理 B 和处理 C 为所有三个处理,和在 CH4 流动和土壤温度之间的弱关系在 CH4 流动和土壤潮湿之间存在。N2O 流动为所有三个处理与土壤温度相关。 | YAN Yuping SHA Liqing CAO Min ZHENG Zheng TANG Jianwei WANG Yinghong ZHANG Yiping WANG Rui LIU Guangren WANG Yuesi SUN Yang | 2008 | Journal of Environmental Sciences2008，20，2： | 18 |
| 2 | Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG显示文摘Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising potential in improving targeting specificity and broadening the targeting range. In this study, we evaluated these Cas9 variants in the model and crop plant, rice. We first tested xCas9-3.7, the most effective xCas9 variant in mammalian cells, for targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates. xCas9 exhibited nearly equivalent editing efficiency to wild-type Cas9 (Cas9-WT) at most canonical NGG PAM sites tested, whereas it showed limited activity at non-canonical NGH (H = A, C, T) PAM sites. High editing efficiency of xCas9 at NGG PAMs was further demonstrated with C to T base editing by both rAPOBECI and PmCDAI cytidine deaminases. With mismatched sgRNAs, we found that xCas9 had improved targeting specificity over the Cas9-WT. Furthermore, we tested two Cas9-NG variants, Cas9-NGv1 and Cas9-NG, for targeting NGN PAMs. Both Cas9-NG variants showed higher editing efficiency at most non-canonical NG PAM sites tested, and enabled much more efficient editing than xCas9 at AT-rich PAM sites such as GAT, GAA, and CAA. Nevertheless, we found that Cas9-NG variants showed significant reduced activity at the canonical NGG PAM sites. In stable transgenic rice lines, we demonstrated that Cas9-NG had much higher editing efficiency than Cas9-NGv1 and xCas9 at NG PAM sites. To expand the base-editing scope, we developed an efficient C to T base-editing system by making fusion of Cas9-NG nickase (D10A version), PmCDAI, and UGI. Taken together, our work benchmarked xCas9 as a high-fidelity nuclease for targeting canonical NGG PAMs and Cas9-NG as a preferred variant for targeting relaxed PAMs for plant genome editing. | Zhaohui Zhong Simon Sretenovic Qiurong Ren Lijia Yang Yu Bao Caiyan Qi Mingzhu Yuan Yao He Shishi Liu Xiaopei Liu Jiaheng Wang Lan Huang Yan Wang Dibin Baby David Wang Tao Zhang Yiping Qi Yong Zhang | 2019 | Molecular Plant2019，12，7： | 13 |
| 3 | Tumor-associated macrophage-derived CCL20 enhances the growth and metastasis of pancreatic cancer显示文摘胰腺的癌症是有导致世界范围的高死亡率的一个高变形的潜力的好攻击的恶意。尽管巨噬细胞有潜力杀死肿瘤房间并且对肿瘤得到有免疫力的回答,有证据,联系肿瘤的巨噬细胞(TAM ) 支持肿瘤前进并且压制 T 房间回答。CC-chemokine ligand 20 (CCL20 ) 并且它的唯一的受体 CC-chemokine 受体(CCR6 ) 6 被癌症房间为移植和转移利用并且在癌症的发展和前进起重要作用。最近的研究证明了 CCL20 的表示是在胰腺的癌症的 upregulated;然而, CCL20 的行动的机制尚待充分被阐明。在这研究,包括变形胰腺的癌症织物,在胰腺的癌症织物的 TAM 的 CCL20 的异常表示被检测。CCL20 表示比在胰腺的癌症房间排队的在巨噬细胞是更加更高的,特别地在 interleukin-4-treated (M2 ) 巨噬细胞。用胰腺的癌症房间的 Boyden 房间试金,我们发现 M2 巨噬细胞分泌的 CCL20 支持了胰腺的癌症房间的迁居,上皮间充质的转变,和侵略。RNA 干扰结果证明 CCR6 是为在胰腺的癌症房间的 CCL20 的受体,调停这些房间的增加的侵略性质由 CCL20 支持了。用一个老鼠模型,我们在在 vivo 支持胰腺的癌症生长和肝转移证实了 CCR6/CCL20 的角色。我们的调查结果在胰腺的癌症提供卓见进藏匿巨噬细胞的 CCL20 的重要角色并且含有 CCR6/CCL20 同样潜在的治疗学的目标。 | Bingyan Liu Yiping Jia Jun Ma Shaoqiu Wu Haosheng Jiang Yan Cao Xianjun Sun Xiang Yin Shuo Yan Mingyi Shang Aiwu Mao | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 13 |
| 4 | Plant Genome Editing Using FnCpfl and LbCpf Nucleases at Redefined and Altered PAM-Sites显示文摘 | ZhaohuiZhong Yingxiao Zhang Qi You XU Tang Qiurong Ren Shishi Liu Lijia Yang Yan Wang Xiaopei Liu Binglin Liu Tao Zhang Xuelian Zheng Ysa Le Yong Zhang Yiping Qi | 2018 | Molecular Plant2018，11，7： | 10 |
| 5 | A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants显示文摘 | Tang, Xu Zheng, Xuelian Qi, Yiping Zhang, Dengwei Cheng, Yan Tang, Aiting Voytas, Daniel F. Zhang, Yong | 2016 | Molecular Plant2016，9，7： | 8 |
| 6 | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method显示文摘This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study. | Changqin Li Yiping Cui Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang | 2021 | Food Science and Human Wellness2021，10，3： | 6 |
| 7 | Origin of strong solid solution strengthening in the CrCoNi-W medium entropy alloy显示文摘Solid solution strengthening is one of the most conventional strategies for optimizing alloys strength,while the corresponding mechanisms can be more complicated than we traditionally thought specifically as heterogeneity of microstructure is involved.In this work,by comparing the change of chemical distribution,dislocation behaviors and mechanical properties after doping equivalent amount of tungsten(W)atoms in CrCoNi alloy and pure Ni,respectively,it is found that the alloying element W in CrCoNi alloy resulted in much stronger strengthening effect due to the significant increase of heterogeneity in chemical distribution after doping trace amount of W.The large atomic scale concentration fluctuation of all elements in CrCoNi-3W causes dislocation motion via strong nanoscale segment detrapping and severe dislocation pile up which is not the case in Ni-3W.The results revealed the high sensitivity of elements distribution in multi-principle element alloys to composition and the significant consequent influence in tuning the mechanical properties,giving insight for complex alloy design. | Yujie Chen Yan Fang Xiaoqian Fu Yiping Lu Sijing Chen Hongbin Bei Qian Yu | 2021 | Journal of Materials Science & Technology2021，，14： | 5 |
| 8 | Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial显示文摘Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across China were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or death.The primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and safety.This study was registered in Clinical Trials.gov(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)group.As of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)months.ORRof responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,respectively.The stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,or recurrent non-squamous NSCLC patients in the first-line setting. | Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni Zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji Chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao LinWu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun Liao Zhiguo Luo Bin Wang Xiaohong Wu Xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong Wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun Chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju Chen Yuan Chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can Wu | 2021 | Cancer Communications2021，41，9： | 5 |
| 9 | National survey of doctor-reported secondary preventive treatment for patients with acute coronary syndrome in China显示文摘 | LI Jing LI Xi Yiping Chen Zhengming Chen ZHANG Li-hua FENG Fang ZHANG Hai-bo LIU Jia-min GAO Yan HUNDEI Wuhan-bilige LI Li JIANG Li-xin | 2013 | Chinese Medical Journal2013，，18： | 5 |
| 10 | Identification and expression of GABAC receptor in rat testis and spermatozoa显示文摘我们的以前的研究显示出那 γ-aminobutyric 酸(伽马氨基丁酸) 和伽马氨基丁酸 B 受体涉及孕酮或伽马氨基丁酸导致的老鼠精子 acrosome 反应。这里,我们报导伽马氨基丁酸 在老鼠睾丸和精子的 C 受体。编码 ρ1, ρ2 和伽马氨基丁酸 C 受体从老鼠睾丸被克隆;他们的序列与老鼠伽马氨基丁酸 在视网膜的 C 受体。反向的抄写聚合酶链反应分析证明在老鼠睾丸的开发期间, ρ1 和 ρ2 子单元的抄本层次显示出小变化,当 ρ3 的表达式是逐渐地起来调整的时。用 anti-ρl 抗体的 Immunofluorescence 分析揭示了那伽马氨基丁酸 C 受体在伸长的 spermatid 和精子上存在。用金霉素试金,我们发现那 N (4 )-chloroacetylcytosine arabinoside,伽马氨基丁酸 C 受体收缩筋,触发的老鼠精子 acrosome 反应;而(1,2,5,6-tetrahydropyridin-4-yl ) methylphosphinic 酸,伽马氨基丁酸 C 受体对手,禁止了 N (4 )-chloroacetylcytosine arabinoside 的能力导致 acrosome 反应。这些结果建议了那伽马氨基丁酸 C 受体也涉及老鼠精子 acrosome 反应。 | Shifeng Li Yunbin Zhang Haixiong Liu Yuanchang Yan Yiping Li | 2008 | Acta Biochimica et Biophysica Sinica2008，40，8： | 4 |
| 11 | Fish species diversity in the Huanghe Estuary显示文摘This paper lists 50 fish specs in the Huanghe Estuary, which belong to 9 orders, 22 families and 37 genera. The paper describes the seasonal distributions of these fish species and the succession of the important fish species during the past 50 a. The authors discuss the main factors that have resulted in the changes in fish species diversity and the fishery resources. These include the overexploitation of the fishery and the sharp reduction in the fresh water discharges from the Huanghe River. | Chen Dagang, Jiao Yan, Liu Qun, Zeng Xiaoqi, Ren Yiping (Department of Fisheries,Ocean University of Qingdao, Qingdao 266003, China.) | 2000 | Acta Oceanologica Sinica2000，19，4： | 4 |
| 12 | Experimental study on water permittivity of woven polypropylene geotextile under tension显示文摘 | Yiping Zhang Weichao Liu Weiyun Shao Yan Yang | 2013 | Geotextiles and Geomembranes2013，，： | 3 |
| 13 | Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes显示文摘推断的一个以前的工作 Enterobacter 下水道的 nifA 基因没恢复 Sinorhizobium meliloti nifA 的共生显型变异。在现在的学习, Bra-dyrhizobium 的 twonifA 基因日本嗯并且 Mesorhizobium huakuii 也没恢复 S 的共生显型。meliloti nifA 异种。在微数组试验的整个 genomic, 238 基因被发现是在 S 以后表示的差别。meliloti nifA 组成地在它的 nifAmutant 被表示了。相反,当表示 B.japonium 时,仅仅 20, 7 和 9 基因改变了他们的 transcriptional 层次, M。在 Sm nifA 异种的 huakuii 和 Enterobacter 下水道 nifA 基因独立。这些基因包括房子保留,精力和中央中间代谢被分类进几官能基,运输系统和共生。有趣地, nifHoperons,显示出的高表示面对任何一个 B 铺平的基因。japonium 或 M。huakuii NifA,它被随后的紫胶 Z 熔化实验证实。 | YAO Zhenhua TIAN Zhexian DAI Xiaomi BECKER Anke LI Jian YAN Haiqin XIAO Yan ZHU Jiabi YU Guanqiao RUVERG Silvia WANG Yiping ZOU Huasong | 2006 | Chinese Science Bulletin2006，51，22： | 2 |
| 14 | Effects of greenness on preterm birth: A national longitudinal study of 3.7 million singleton births显示文摘Exposure to greenness may lead to a wide range of beneficial health outcomes.However,the effects of greenness on preterm birth(PTB)are inconsistent,and limited studies have focused on the subcategories of PTB.A total of 3,751,672 singleton births from a national birth cohort in China's Mainland were included in this study.Greenness was estimated using the satellitebased Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index with 500-m and 1,000-m buffers around participants’addresses.The subcategories of PTB(20-36 weeks)included extremely PTB(EPTB,20-27 weeks). | Lina Zhang Shuyan Shi Shenpeng Wu Ying Yang Jihong Xu Ya Zhang Qiaomei Wang Haiping Shen Yiping Zhang Donghai Yan Zuoqi Peng Cong Liu Weidong Wang Yixuan Jiang Su Shi Renjie Chen Haidong Kan Yuan He Xia Meng Xu Ma | 2022 | The Innovation2022，3，3： | 2 |
| 15 | One-pot synthesis of silver colloid with body-heat for surface-enhanced Raman spectroscopy detections显示文摘In this study,a convenient method of preparing the substrate is proposed with one-pot synthesis of silver colloid under body heat,and the SERS detection uses the fresh substrate to avoid the drawback of substrates’short life of use.The synthesis of silver colloid is carried out in a 10 mL vial by using ascorbic acid as a reductant and trisodium citrate as a stabilizer.The vial is grasped with the palm of the experimenter for several minutes without shaking.The proposed method is simple,rapid,green energy and cost-effective.By adjusting the concentration of trisodium citrate,not only the particle size can be controlled from about 110 nm to 50 nm but also the homogeneity of nanoparticles can be improved.As a SERS substrate,the silver colloid has high batch reproducibility and showed good SERS activity.The relative standard deviation between different manufacturers is 5.51%when the substrate of silver colloid is used for the detection of rhodamine 6 G.Using the substrate,the lowest detection concentrations of rhodamine 6 G,crystal violet,enrofloxacin,melamine and leucomalachite green are 1.0×10-8,6.1×10-8,1.4×10-6,7.1×10-5 and 5.1×10-8 mol/L,respectively.Results demonstrate that the developed method has the advantage of convenience and high efficiency in the field preparation of reliable SERS substrate. | Wanchao Chen Yan Kang Han Zhang Tianxiong Huang Xin Tao Aiping Lu Yiping Du | 2019 | Chinese Chemical Letters2019，30，5： | 2 |
| 16 | Atmospheric emissions estimation of Hg, As, and Se from coal-fired power plants in China, 2007显示文摘 | Hezhong Tian Yan Wang Zhigang Xue Yiping Qu Fahe Chai Jiming Hao | 2011 | Science of the Total Environment2011，，16： | 2 |
| 17 | Highly efficient stacked organic light-emitting devices employing a novel intermediate layer显示文摘Highly efficient stacked organic light-emitting devices (OLEDs) have been fabricated using Mg:Alq3:BCP/ WO3 as a novel intermediate layer, which connects two identical emissive units. The stacked OLEDs consisting of NPB/Alq3 exhibited high current efficiency and brightness characteristics over conven- tional single-unit device. At 200 mA/cm2, the current efficiency of the stacked OLEDs using the inter- mediate layers of Mg:Alq3:BCP/WO3 were about 6.85 cd/A, doubling that of the conventional device. The results may prove the method to be effective to enhance the current efficiency and brightness of OLEDs. | NIU LianBin GUAN YunXia REN Yue KONG ChunYang MA Yan LIANG YiPing | 2009 | Science China(Technological Sciences)2009，52，1： | 2 |
| 18 | Experiments and simulations of gas-solid flow in an airlift loop reactor显示文摘The hydrodynamics in a gas-solid airlift loop reactor was investigated systematically using experimental measurements and CFD simulation.In the experiments,the time averaged parameters,such as solid fraction and particle velocity,were measured by optical fiber probe.In the simulation,the modified Gidaspow drag model accounting for the interparticles clustering was incorporated into the Eulerian-Eulerian CFD model with particulate-phase kinetic theory.Predicted values of solid fraction and particle velocity were compared with experimental results,validating the drag model and the simulation.The results show that the profiles of particle velocity and solid fraction are uniform in annulus.However,the core-annulus structure appears in other three regions (draft tube region,bottom region and particle diffluence region),which presents the similar heterogeneous feature of aggregative fluidization usually occurred in normal fluidized beds.Simulated profiles of particle residence time distribution indicate that the airlift loop reactor should be characterized by near perfect mixing. | Chaoyu Yan Chunxi Lu Yiping Fan Rui Cao Yansheng Liu | 2011 | Particuology2011，9，2： | 2 |
| 19 | Two Ultraviolet Radiation Datasets that Cover China显示文摘Ultraviolet(UV) radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization's measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. | Hui LIU Bo HU Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU | 2017 | Advances in Atmospheric Sciences2017，34，7： | 2 |
| 20 | An imputed whole-genome sequence-based GWAS approach pinpoints causal mutations for complex traits in a specific swine population显示文摘Sequencing-based genome-wide association studies(GWAS) have facilitated the identification of causal associations between genetic variants and traits in diverse species. However, it is cost-prohibitive for the majority of research groups to sequence a large number of samples. Here, we carried out genotype imputation to increase the density of single nucleotide polymorphisms in a large-scale Swine F;population using a reference panel including 117 individuals, followed by a series of GWAS analyses. The imputation accuracies reached 0.89 and 0.86 for allelic concordance and correlation, respectively. A quantitative trait nucleotide(QTN) affecting the chest vertebrate was detected directly, while the investigation of another QTN affecting the residual glucose failed due to the presence of similar haplotypes carrying wild-type and mutant allelesin the reference panel used in this study. A high imputation accuracy was confirmed by Sanger sequencing technology for the most significant loci. Two candidate genes,CPNE5 and MYH3, affecting meat-related traits were proposed. Collectively, we illustrated four scenarios in imputation-based GWAS that may be encountered by researchers, and our results will provide an extensive reference for future genotype imputation-based GWAS analyses in the future. | Guorong Yan Xianxian Liu Shijun Xiao Wenshui Xin Wenwu Xu Yiping Li Tao Huang Jiangtao Qin Lei Xie Junwu Ma Zhiyan Zhang Lusheng Huang | 2022 | Science China(Life Sciences)2022，65，4： | 2 |
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