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| 1 | One-step base editing in multiple genes by direct embryo injection for pig trait improvement显示文摘The precise and simultaneous acquisition of multiple beneficial alleles in the genome is in great demand for the development of elite pig breeders. Cytidine base editors(CBEs) that convert C:G to T:A have emerged as powerful tools for single-nucleotide replacement. Whether CBEs can effectively mediate C-to-T substitution at multiple sites/loci for trait improvement by direct zygote injection has not been verified in large animals. Here, we determined the editing efficiency of four CBE variants in porcine embryonic fibroblast cells and embryos. The findings showed that hA3A-BE3-Y130F and hA3A-eBE-Y130F consistently resulted in increased base-editing efficiency and low toxic effects in embryonic development. Further, we verified that using a one-step approach, direct zygote microinjection of the CBE system can generate pigs harboring multiple point mutations.Our process resulted in a stop codon in CD163 and myostatin(MSTN) and introduced a beneficial allele in insulin-like growth factor-2(IGF2). The pigs showed disrupted expression of CD163 and MSTN and increased expression of IGF2, which significantly improved growth performance and infectious disease resistance. Our approach allows immediate introduction of multiple mutations in transgene-free animals to comprehensively improve economic traits through direct embryo microinjection,providing a potential new route to produce elite pig breeders. | Ruigao Song Yu Wang Qiantao Zheng Jing Yao Chunwei Cao Yanfang Wang Jianguo Zhao | 2022 | Science China(Life Sciences)2022，65，4： | 9 |
| 2 | A PSO based Energy Efficient Coverage Control Algorithm for Wireless Sensor Networks显示文摘Wireless Sensor Networks(WSNs)are large-scale and high-density networks that typically have coverage area overlap.In addition,a random deployment of sensor nodes cannot fully guarantee coverage of the sensing area,which leads to coverage holes in WSNs.Thus,coverage control plays an important role in WSNs.To alleviate unnecessary energy wastage and improve network performance,we consider both energy efficiency and coverage rate for WSNs.In this paper,we present a novel coverage control algorithm based on Particle Swarm Optimization(PSO).Firstly,the sensor nodes are randomly deployed in a target area and remain static after deployment.Then,the whole network is partitioned into grids,and we calculate each grid’s coverage rate and energy consumption.Finally,each sensor nodes’sensing radius is adjusted according to the coverage rate and energy consumption of each grid.Simulation results show that our algorithm can effectively improve coverage rate and reduce energy consumption. | Jin Wang Chunwei Ju Yu Gao Arun Kumar Sangaiah Gwang-jun Kim | 2018 | Computers, Materials & Continua2018，，9： | 6 |
| 3 | FAK-targeting PROTAC as a chemical tool for the investigation of non-enzymatic FAK function in mice显示文摘Dear Editor,Animal models,most commonly mice,that lack a protein of interest play an important role in phenotypic and functional studies of a target gene,allowing researchers to answer various biological questions(Chaible et al,2010).At pre-sent,a variety of tools act at the DNA or RNA level to enable researchers to model gene function(and thus protein)deficiency,including nucleic acid based RNA interference(EI-bashir et al.,2001),antisense oligonucleotides(Schoch and Miller,2017),and genome editing-based CRISPR-Cas9(Doudna and Charpentier,2014)strategies.However,challenges remain. | Hongying Gao Chunwei Zheng Jian Du Yue Wu Yonghui Sun Chunsheng Han Kehkooi Kee Yu Rao | 2020 | Protein & Cell2020，11，7： | 5 |
| 4 | Changes of Extreme Precipitation and its Associated Mechanisms in Northwest China显示文摘Characterized by scarce water resources and fragile ecosystems,Northwest China(NWC)has experienced a climate shift from warm-dry to warm-wet conditions since the 1980s that has garnered extensive concern in recent years.In this study,the variability in extreme precipitation(EP)during 1961-2016 in different climate zones of NWC and the possible mechanisms for this variation are investigated.The results show that the EP trends significantly increased in most of the westerly zone(WZ)and plateau zone(PZ),while the EP trends did not significantly decrease in the monsoon zone(MZ).The start dates of extreme precipitation(SDEP)and end dates of extreme precipitation(EDEP)advanced and were postponed,respectively,in the WZ and PZ,while the opposite occurred in the MZ.Summer atmospheric circulation,water vapor transport,and atmospheric instability over NWC varied greatly with the interdecadal shift in EP before and after 1986.During 1986-2016,upper-level divergence and lower-level convergence occurred in the MZ and PZ,which strengthened ascending flow.In addition,the summer water vapor and atmospheric instability increased in the WZ and PZ.These characteristics created favorable conditions for increased occurrences of EP in the WZ and PZ in summer.Conversely,the upper-level convergence and lower-level divergence in the MZ strengthened descending flow.Decreases in summer water vapor and atmospheric instability occurred in the MZ after 1986.Hence,the environmental conditions in the MZ may have prevented the occurrence and development of EP in summer during 1986-2016. | Shan LU Zeyong HU Haipeng YU Weiwei FAN Chunwei FU Di WU | 2021 | Advances in Atmospheric Sciences2021，38，10： | 4 |
| 5 | N-1 -(2-Mercaptoethyl)thymine modification of gold nanoparticles : a hi^ily selective andsensitive colorimetric chemosensor for Hg2+ 显示文摘 | Ling Chen Tingting Lou Chunwei Yu | 2011 | Analyst2011，，136： | 1 |
| 6 | Neutralization of interleukin-17A delays progression of silica-induced lung inflammation and fibrosis in C57BL/6 mice显示文摘 | Ying Chen Cuiying Li Dong Weng Laiyu Song Wen Tang Wujing Dai Ye Yu Fangwei Liu Ming Zhao Chunwei Lu Jie Chen | 2013 | Toxicology and Applied Pharmacology2013，，： | 1 |
| 7 | Fluorescent probe for copper( ] ) ion based on a rhodamine spirolactame derivative,and its application to fluorescent imaging in living cells显示文摘 | Yu Chunwei Zhang Jun Li Jinhua | 2011 | Microchim Acta2011，174，34： | 1 |
| 8 | Morphology and co- agulation performance during preparation of poly-silic- ic-ferric (PSF) coagulant 显示文摘 | Fu Ying Yu Shuili Han Chunwei | 2009 | Chemical Engineering Journal2009，149，13： | 1 |
| 9 | African swine fever virus MGF505-3R inhibits cGAS-STING-mediated IFN-βpathway activation by degrading TBK1显示文摘African swine fever virus(ASFV)is an important pathogen causing acute infectious disease in domestic pigs and wild boars that seriously endangers the global swine industry.As ASFV is structurally complex and encodes a large number of functional proteins,no effective vaccine has been developed to date.Thus,dissecting the mechanisms of immune escape induced by ASFV proteins is crucial.A previous study showed that the ASFV-encoded protein is an important factor in host immunity.In this study,we identified a negative regulator,MGF505-3R,that significantly downregulated cGAS/STING-and poly(dG:dC)-mediated IFN-βand interferon stimulation response element(ISRE)reporter activity and suppressed IFNB1 and IFIT2 mRNA levels.In addition,TBK1,IRF3 and IκBαphosphorylation levels were also inhibited.Mechanistically,MGF505-3R interacted with cGAS/TBK1/IRF3 and targeted TBK1 for degradation,thereby disrupting the cGAS-STING-mediated IFN-βsignaling pathway,which appears to be highly correlated with autophagy.Knockdown MGF505-3R expression enhanced IFN-βand IL-1βproduction.Taken together,our study revealed a negative regulatory mechanism involving the MGF505-3R-cGAS-STING axis and provided insights into an evasion strategy employed by ASFV that involves autophagy and innate signaling pathways. | Mingyang Cheng Jiawei Luo Yuetong Duan Yu Yang Chunwei Shi Yu Sun Yiyuan Lu Junhong Wang Xiaoxu Li Jianzhong Wang Nan Wang Wentao Yang Yanlong Jiang Guilian Yang Yan Zeng Chunfeng Wang Xin Cao | 2022 | Animal Diseases2022，2，3： | 1 |
| 10 | Morphology and coagula- tion performance during preparation of poly-silicic-ferric (PSF) coagulant显示文摘 | Fu Ying Yu Shuili Han Chunwei | 2009 | Chemical Engineering Journal2009，149，： | 1 |
| 11 | 显示文摘 | Liang Bingjie Gan Lin yuan Chunwei Wei Yu | 1998 | Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy1998，54，1： | 1 |
| 12 | Obesity paradox: Clinical benefits not observed in obese patients with ST-segment elevation myocardial infarction: A multicenter, prospective, cohort study of the northern region of China显示文摘 | Yuze Li Chunwei Wu Yujiao Sun Daming Jiang Bo Zhang Lina Ren Yuan Gao Haijie Yu Guang Yang Qigang Guan Wen Tian Haishan Zhang Liang Guo Guoxian Qi | 2013 | International Journal of Cardiology2013，，： | 1 |
| 13 | Correlation between brazed joint elevated strength,microstructure and brazing processing parameter of Inconel superalloy显示文摘This study investigates the influences of brazing temperature,brazing time and brazing clearance on microstructures and high temperature strength of Inconel superalloy.Bonding is performed in a high vacuum furnace using BNi-2 as filler metal.Brazing temperatures employed in this study are 1080 ℃,1110 ℃ and 1140 ℃.Holding times at the brazing temperature are 5 min,15 min and 45 min.At the same time,the investigated brazing clearances are 30 μm,60 μm and 100 μm.Microstructure of the brazed joints is analyzed by means of metallography,scanning electron microscope (SEM).The high temperature tensile strength and microhardness are evaluated at different brazing parameters.The results show that fracture occurs wholly within the braze metal.Deformation appears to be confined to the braze metal with the base metal,showing very little plastic deformation.Brazing time shows to play the important role in the brazing parameters.The peak of microhardness is at the centerline of braze region.Induction heating has little effect on the base materials. | Chunwei MA Kun SHI Zhishui YU Peiquan XU | 2011 | Acta Metallurgica Sinica(English Letters)2011，24，3： | 0 |
| 14 | Form Finding and Collapse Analysis of Cable Nets Under Dynamic Loads Based on Finite Particle Method显示文摘This paper presents form finding and collapse analysis of cable net structure under strong wind using the finite particle method(FPM).As a kind of particle method,the theoretical fundamentals of the FPM are given.Methods to handle geometric and material nonlinearities of cable element are proposed.The fracture criterion and model for cable element are built to simulate the failure of cable nets.The form-finding and load analysis of two cable nets are then performed in order to initialize the successive of nonlinear analysis.The failure progress of cable nets under dynamic loads is simulated,and the dynamic responses of the typical fracture element are given in details.Analyses of the energy variations during the collapse process also show the failure mechanisms of cable nets,which is useful for the structure collapse resistance design.The numerical applications highlight the capability of the proposed procedure to solve complicate collapse problems with the FPM. | Ying Yu Ping Xia Chunwei Yang | 2018 | Computer Modeling in Engineering & Sciences2018，，10： | 0 |
| 15 | Numerical Simulation of Flow between Rotating Cylinders显示文摘IntroductionFlowbetweenrotatingcylinderswithsmalh/R(histhegapbetweencylindersandRistheinnercylinderradius)hasbeenwidelystudie... | Chunwei YU and Guocan LING (LNM, Institute of Mechanics, CAS, Beijing, 100080, China) | 1998 | Communications in Nonlinear Science and Numerical Simulation1998，3，1： | 0 |
| 16 | Enhancement of Organic Electrolum Inescent Intensity by Doping Hole-Property Polymers显示文摘Organic electroluminescent(EL)diodes with one-layer poly(3-octylthiophene)(P3OT)and P3OT doped with poly(N-vinylcarbazole)(PVK)(P3OT/PVK)as EL emitting layer sandwiched between indium/tin oxide(lTO)and aluminium electrodes have been fabricated by spin coating onto IT0 respectively.The P3OT/PVK EL diode starts to emit red-orange light at 5 V in our experiment,the luminance and the current for the P3OT/PVK EL diode at bias voltage was compared with those for the only P3OT EL diode,indicating that the PVK dopant has power over the P3OT electroluminescence. | WANG Guangming WU Haiming YUAN Chunwei WEI Yu | 1995 | Chinese Physics Letters1995，12，9： | 0 |
| 17 | Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state显示文摘During homeostasis and after injury,adult muscle stem cells(MuSCs)activate to mediate muscle regeneration.However,much remains unclear regarding the heterogeneous capacity of MuSCs for self-renewal and regeneration.Here,we show that Lin28a is expressed in embryonic limb bud muscle progenitors,and that a rare reserve subset of Lin28a^(+)Pax7^(-)skeletal MuSCs can respond to injury at adult stage by replenishing the Pax7^(+)MuSC pool to drive muscle regeneration.Compared with adult Pax7^(+)MuSCs,Lin28a^(+)MuSCs displayed enhanced myogenic potency in vitro and in vivo upon transplantation.The epigenome of adult Lin28a^(+)MuSCs showed resemblance to embryonic muscle progenitors.In addition,RNA-sequencing revealed that Lin28a^(+)MuSCs co-expressed higher levels of certain embryonic limb bud transcription factors,telomerase components and the p53 inhibitor Mdm4,and lower levels of myogenic differentiation markers compared to adult Pax7^(+)MuSCs,resulting in enhanced self-renewal and stress-response signatures.Functionally,conditional ablation and induction of Lin28a^(+)MuSCs in adult mice revealed that these cells are necessary and sufficient for efficient muscle regeneration.Together,our findings connect the embryonic factor Lin28a to adult stem cell self-renewal and juvenile regeneration. | Peng Wang Xupeng Liu Ziyue Yao Yu Chen Lanfang Luo Kun Liang Jun-Hao Elwin Tan Min-Wen Jason Chua Yan-Jiang Benjamin Chua Shilin Ma Liping Zhang Wenwu Ma Shuqing Liu Wenhua Cao Luyao Guo Lu Guang Yuefan Wang He Zhao Na Ai Yun Li Chunwei Li Ruiqi Rachel Wang Bin Tean Teh Lan Jiang Kang Yu Ng Shyh-Chang | 2023 | Cell Research2023，33，9： | 0 |
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