|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Salvianolic acid A alleviates renal injury in systemic lupus erythematosus induced by pristane in BALB/c mice显示文摘The purpose of this study was to investigate the effects of salvianolic acid A(SAA) in systemic lupus erythematosus(SLE) induced by pristane in BALB/c mice.Lupus mice were established by confirming elevated levels of autoantibodies and IL-6 after intraperitoneal injection of pristane.Mice were then treated with daily oral doses of SAA for 5 months in parallel with mice treated with prednisone and aspirin as positive controls.The levels of autoantibodies were monitored at monthly intervals and nephritic symptoms observed by hematoxylin and eosin(H&E) and periodic acid-Schiff(PAS) staining.Western blot analysis of renal tissue was also employed.SAA treatment caused a significant reduction in the levels of anti-Sm autoantibodies and reduced renal histopathological changes and pathological effects.SAA treatment also significantly inhibited the phosphorylation of IKK,IκB and NFκB in renal tissues of lupus mice.In conclusion,the results suggest that SAA alleviates renal injury in pristane-induced SLE in BALB/c mice through inhibition of phosphorylation of IKK,IκB and NFκB. | Yihuang Lin Yu Yan Huifang Zhang Yucai Chen Yangyang He Shoubao Wang Lianhua Fang Yang Lv Guanhua Du | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 8 |
| 2 | Two-acceleration-error-input proportional-integral-derivative control for vehicle active suspension显示文摘The objective of this work is to present a new two-acceleration-error-input(TAEI) proportional-integral-derivative(PID) control strategy for active suspension.The novel strategy lies in the use of sprang mass acceleration and unsprung mass acceleration signals simultaneously,which are easily measured and obtained in engineering practice.Using a quarter-car model as an example,a TAEI PID controller for active suspension is established and its control parameters are optimized based on the genetic algorithm(GA),in which the fitness function is a suspension quadratic performance index.Comparative simulation shows that the proposed TAEI PID controller can achieve better comprehensive performance,stability,and robustness than a conventional single-acceleration-error-input(SAEI) PID controller for the active suspension. | Yucai Zhou Shian Chen Juncheng Wang | 2014 | Journal of Traffic and Transportation Engineering(English Edition)2014，1，3： | 8 |
| 3 | Noble gases in corundum megacrysts from the basalts in Changle,Shandong Province,eastern China显示文摘This study presents noble gaseous data of the corundum megacrysts from the Cenozoic basalts in Changle, Shandong Province, eastern China. It is known that no noble gaseous data of corundum megacryst have been documented before. The 3He/4He ratios (1.13-7.37 Ra) of the corundums from Changle vary from atmosphere to MORB values; the 20Ne/22Ne (9.67-10.75) and 21Ne/22Ne (0.0280-0.0372) data define two linear trends on Ne three-isotope diagram, respectively, along the MFL and the correlation line between atmosphere and MORB; the 38Ar/36Ar (0.177-0.194) ratios, the 40Ar/36Ar (280.9 -404.2) ratios and the 128-136Xe/132Xe ration with obvious 129Xe excess are generally higher than at-mospheric component, but the 40Ar/36Ar ratios are much closer to atomospheric ratio. The isotopic compositions of noble gases (particularly for He and Ar) of the corundums are similar to those of py-roxene, anorthoclase megacrysts, and mantle-derived xenoliths from this area, and those of man-tle-derived xenoliths from several areas in eastern China. Therefore, the noble gases trapped in the corundums probably are from mantle source, representing a ‘mixed fluid' produced by the interaction between the lithospheric mantle and fluids releasing from the convective plate. Both the noble gas isotopic compositions and the oxygen isotopic compositions of the solid corundums are not the characteristics of crustal source. These suggest that the corundums crystallized from mantle-derived magmas with minimal crustal contamination. | HU WenXuan SONG YuCai CHEN XiaoMing TAO MingXin ZHANG LiuPing | 2007 | Chinese Science Bulletin2007，52，3： | 6 |
| 4 | First experimental constraints on WIMP couplings in the effective field theory framework from CDEX显示文摘We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China Dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region. | Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan Chen YunHua Chen JianPing Cheng Cheng Yi Chiang WenHan Dai Zhi Deng ChangHao Fang XinPing Geng Hui Gong QiuJu Guo XuYuan Guo HongJian He Li He ShengMing He JinWei Hu TuChen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li MingXuan Li RenMingJie Li Xia Li YuLan Li Bin Liao FongKay Lin ShinTed Lin ShuKui Liu YanDong Liu Yu Liu YuanYuan Liu ZhongZhi Liu Hao Ma YuCai Mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang WeiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu Chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue Ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang Zhao MingGang Zhao JiFang Zhou ZuYing Zhou JingJun Zhu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，8： | 3 |
| 5 | A multicenter retrospective cohort study of ketogenic diet therapy in 481 children with infantile spasms显示文摘Background:Ketogenic diet(KD)therapy is one of the main treatments for drug-resistant epilepsy.However,the KD therapy has been applied in only a small number of infantile spasm cases.In this large multicenter study,we investigated the efficacy of KD therapy in the treatment of infantile spasms.Methods:In this retrospective,multicenter cohort study,clinical data from main epilepsy centers were analyzed.Patients were classified into different groups according to age,type of drug and whether glucocorticoid was used before initiation of KD.Results:From October 2014 to March 2020,481 patients(308 males and 173 females)with infantile spasms were treated with the KD therapy.The age of the patients ranged from 2 months to 20 years,with a mean age of 1 year and 10 months.The number of anti-seizure medications(ASMs)used before KD initiation ranged 0-6,with a median of 3.In different time from initiation(1,3,6,and 12 months),the rates of seizure freedom after KD were 6.9,11.6;16.0 and 16.8%,respectively(x^(2)=27.1772,P<0.0001).There was a significant difference in the rate of seizure freedom between 3 months and 1 month(x^(2)=6.5498,P=0.0105)groups,and 6 months and 3 months(x^(2)=3.8478,P=0.0498)groups,but not between 12 months and 6 months(x^(2)=0.1212,P=0.7278)groups.The rates of effectiveness were 44.7;62.8,49.1 and 32.0%(x^(2)=93.2674,P<0.0001),respectively.The retention rates were 94.0,82.5,55.7 and 33.1%(x^(2)=483.7551,P<0.0001),correspondingly.The rate of effectiveness and the retention rate of KD were significantly different among the 1,3,6 and 12 months.KD treatment was the first choice in 25 patients(5.2%),55 patients(11.4%)started KD after the failure of the first ASM,158 patients(32.8%)started KD after the failure of the second ASM,157 patients(32.6%)started KD after the failure of the third drug,and 86 patients(17.9%)started KD after the failure of the fourth and more.The KD effect was not related to the number of ASMs used before KD startup(P>0.05).Two hundred and eighteen patients(45.3%)failed to respond to corticotropin or glucocorticoid before initiation.There was no significant difference in the effectiveness rate at different time points between the group of KD therapy after glucocorticoid failure and the group after non-hormone failure (x^(2)=0.8613,P=0.8348).The rate of adverse events of KD in 1,3,6,and 12 months after KD initiation were 22.3,21.7,16.8 and 6.9%,respectively.The adverse events mainly occurred during the first 3 months of KD,and the main adverse events were gastrointestinal disturbance and constipation.Conclusions:The efficacy of the KD treatment for infantile spasms was not affected by age,medication,and glucocorticoid use before initiation.KD is one of the effective treatments for infantile spasms. | Yuanzhen Ye Dan Sun Hua Li Jianmin Zhong Rong Luo Baomin Li Dengna Zhu Dan Li Shaoping Huang Yuwu Jiang Nong Xiao Yucai Chen Yuqin Zhang Mei Yu Xiaoyun Shen Li Gao Guo Zheng Congmin Zhao Baoqiang Yuan Jianxiang Liao Jiong Qin CAAE KD group | 2022 | Acta Epileptologica2022，5，1： | 2 |
| 6 | Plant base editing and prime editing:The current status and future perspectives显示文摘Precise replacement of an allele with an elite allele controlling an important agronomic trait in a predefined manner by gene editing technologies is highly desirable in crop improvement.Base editing and prime editing are two newly developed precision gene editing systems which can introduce the substitution of a single base and install the desired short indels to the target loci in the absence of double-strand breaks and donor repair templates,respectively.Since their discoveries,various strategies have been attempted to optimize both base editor(BE)and prime editor(PE)in order to improve the precise editing efficacy,specificity,and expand the targeting scopes.Here,we summarize the latest development of various BEs and PEs,as well as their applications in plants.Based on these progresses,we recommend the appropriate BEs and PEs for both basic plant research and crop improvement.Moreover,we propose the perspectives for further optimization of these two editors.We envision that both BEs and PEs will become the routine and customized precise gene editing tools for both plant biological research and crop improvement in the near future. | Jingying Li Chen Zhang Yubing He Shaoya Li Lei Yan Yucai Li Ziwei Zhu Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，2： | 1 |
| 7 | Tumoricid- al effects of a selenium (Se)-polysaccharide from Ziyang green tea on human osteosarcoma U-2 OS cells 显示文摘 | Yucai Wang Jun Chen Dianzhong Zhang | 2013 | Carbo- hydrate Polymers2013，1，98： | 1 |
| 8 | p21 overexpression sensitizes osteosarcoma U2OS cells to cisplatin via evoking caspase-3 and Bax/Bcl-2 cascade显示文摘 | Yong Ding Yucai Wang Jun Chen Yunsheng Hu Zhuo Cao Pengcheng Ren Yong Zhang | 2014 | Tumor Biology2014，，4： | 1 |
| 9 | Inter-rater reliability of the A-S-C-O classification system for ischemic stroke显示文摘 | Ning Chen Muke Zhou Yucai Wang Hui Wang Mi Yang Jian Guo Xue Yang Hongbo Zheng Dong Zhou Li He | 2012 | Journal of Clinical Neuroscience2012，，： | 1 |
| 10 | Microwave-induced synthesis and characterization of nanometer Ce_(0.5)Zr_(0.5)O_2 solid solution for the acidic catalytic reaction显示文摘Ce0.5Zr0.5O2 稳固的答案被微波照耀方法成功地作为原料用铈硝酸盐,锆硝酸盐,和脲综合并且由 X 光检查衍射,荧光光谱,传播电子显微镜学,和红外线的光谱描绘了。它的酸催化活动在醋酸和 n 丁基酒精的酯化作用反应被评估。结果证明那个 Ce0.5Zr0.5O2 稳固的答案有立方的荧石结构,并且它的粒子直径在纳米规模。作为一种稳固的酸,它拥有更高的酸催化活动和罐头容易与反应液体被分开。它能若干次被用于,并且基本上,它的活动保持不变。质子酸地点和路易斯酸地点在 Ce0.5Zr0.5O2 稳固的答案的结构存在。 | HU Yucai YIN Ping LIANG Tao JIANG Wei DU Zhengkun CHEN Yonghua | 2008 | Rare Metals2008，27，2： | 1 |
| 11 | Activating transcription factor 4 protects mice against sepsis-induced intestinal injury by regulating gut-resident macrophages differentiation显示文摘Background: Gut-resident macrophages (gMacs) supplemented by monocytes-to-gMacs differentiation play a critical role in maintaining intestinal homeostasis. Activating transcription factor 4 (ATF4) is involved in immune cell differentiation. We therefore set out to investigate the role of ATF4-regulated monocytes-to-gMacs differentiation in sepsis-induced intestinal injury.Methods: Sepsis was induced in C57BL/6 wild type (WT) mice andAtf4-knockdown (Atf4+/-) mice by cecal ligation and puncture or administration of lipopolysaccharide (LPS). Colon, peripheral blood mononuclear cells, sera, lung, liver, and mesenteric lymph nodes were collected for flow cytometry, hematoxylin and eosin staining, immunohistochemistry, quantitative reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively.Results: CD64, CD11b, Ly6C, major histocompatibility complex-II (MHC-II), CX3CR1, Ly6G, and SSC were identified as optimal primary markers for detecting the process of monocytes-to-gMacs differentiation in the colon of WT mice. Monocytes-to-gMacs differentiation was impaired in the colon during sepsis and was associated with decreased expression of ATF4 in P1 (Ly6Chi monocytes), the precursor cells of gMacs.Atf4 knockdown exacerbated the impairment of monocytes-to-gMacs differentiation in response to LPS, resulting in a significant reduction of gMacs in the colon. Furthermore, compared with WT mice,Atf4+/- mice exhibited higher pathology scores, increased expression of inflammatory factor genes (TNF-α, IL-1β), suppressed expression of CD31 and vascular endothelial-cadherin in the colon, and increased translocation of intestinal bacteria to lymph nodes and lungs following exposure to LPS. However, the aggravation of sepsis-induced intestinal injury resulting fromAtf4 knockdown was not caused by the enhanced inflammatory effect of Ly6Chi monocytes and gMacs.Conclusion: ATF4, as a novel regulator of monocytes-to-gMacs differentiation, plays a critical role in protecting mice against sepsis-induced intestinal injury, suggesting that ATF4 might be a potential therapeutic target for sepsis treatment. | Zhenliang Wen Xi Xiong Dechang Chen Lujing Shao Xiaomeng Tang Xuan Shen Sheng Zhang Sisi Huang Lidi Zhang Yizhu Chen Yucai Zhang Chunxia Wang Jiao Liu | 2022 | Chinese Medical Journal2022，135，21： | 1 |
| 12 | Predictive analytics with ensemble modeling in laparoscopic surgery:A technical note显示文摘Predictive analytics have been widely used in the literature with respect to laparoscopic surgery and risk stratification.However,most predictive analytics in this field exploit generalized linearmodels for predictive purposes,which are limited by model assumptionsdincluding linearity between response variables and additive interactions between variables.In many instances,such assumptions may not hold true,and the complex relationship between predictors and response variables is usually unknown.To address this limitation,machine-learning algorithms can be employed to model the underlying data.The advantage of machine learning algorithms is that they usually do not require strict assumptions regarding data structure,and they are able to learn complex functional forms using a nonparametric approach.Furthermore,two or more machine learning algorithms can be synthesized to further improve predictive accuracy.Such a process is referred to as ensemble modeling,and it has been used broadly in various industries.However,this approach has not been widely reported in the laparoscopic surgical literature due to its complexity in both model training and interpretation.With this technical note,we provide a comprehensive overview of the ensemble-modeling technique and a step-by-step tutorial on how to implement ensemble modeling. | Zhongheng Zhang Lin Chen Ping Xu Yucai Hong | 2022 | Laparoscopic, Endoscopic and Robotic Surgery2022，5，1： | 1 |
| 13 | Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response显示文摘FANCM/FAAP24 heterodimer 在保护房间免受象 interstrand 交叉连接那样的复杂 DNA 损害的伤害有不同功能。这些功能依靠 FANCM/FAAP24 的生物化学的活动认出并且绑在损坏 DNA 或阻止的复制叉。然而,这建筑群的 DNA 有约束力的活动清楚地没被定义。我们调查了 FAAP24 怎么由获得人的 FAAP24 的 N 终端和 C 终端解决方案结构贡献 FANCM/FAAP24 建筑群的交往 DNA 函数。FAAP24 结构当模特儿显示 FAAP24 可以向搁浅单人赛的 DNA (ssDNA ) 拥有一种高亲密关系。在 vitro 并且在 vivo 各种各样的 FAAP24 变化测试验证了从结构的分析导出的这预言。我们发现 DNA 有约束力并且尽管两个要求 C 终端(HhH )2 领域, FAAP24 的交往 FANCM 功能能被 segregation-of-function 变化区分。这些结果证明在 DNA 的 FAAP24 的双角色对 crosslinking 损害损坏反应,通过经由它的 ssDNA 有约束力的活动的 FANCM/FAAP24 heterodimer 和其它的形成的在优化检查点激活要求了。 | Yucai Wang Xiao Han Fangming Wu Justin W Leung Megan G Lowery Huong Do Junjie Chen Chaowei Shi Changlin Tian Lei Li Weimin Gong | 2013 | Cell Research2013，23，10： | 1 |
| 14 | Production safety evaluation model based on principal component analysis 显示文摘 | Hongmei Chen Yucai Ning Xudong Sun | 2011 | Procedia Engineering2011，26，： | 1 |
| 15 | High drug loading and pH-responsive nanomedicines driven by dynamic boronate covalent chemistry for potent cancer immunotherapy显示文摘Tumor cells undergoing immunogenic cell death (ICD) have emerged as an in situ therapeutic vaccine helping to activate a persistent anti-tumor response. Several chemotherapeutic agents have been demonstrated to induce ICD, however accompanied with severe adverse effects in the clinic, weakening its immune responses. Herein, to elicit an intensive ICD while minimizing the systemic toxicity, we introduce a tumor targeting peptide modified bortezomib (BTZ) loading nanomedicine (i-NPBTZ) for the efficient delivery and controlled release of BTZ in tumors. This system is constructed by conjugating BTZ to PEGylated polyphenols via a pH-sensitive covalent boronate-phenol bond that allows them to self-assemble into nanovesicles in neutral condition with high drug loading efficiency. Once accumulated in acidic environment, BTZ-phenolic network is disassembled and thereby accelerates the release of BTZ from nanocarriers. The released BTZ selectively kill tumor cells with a concomitant evocation of tumor-specific cytotoxic T cells by triggering ICD in vivo. This can finally lead to an extended tumor ablation and prevention of distant metastasis in a syngeneic tumor mouse model, while reducing the systemic toxicity of BTZ. In general, our system offers a novel concept with clinical potential to exploit ICD for potentiating tumor immunotherapy and also provides an excellent example of the application of polymer-drug interaction for efficient drug delivery and controllable release. | Wei Jiang Han Zhou Qin Wang Ziqi Chen Wang Dong Zixuan Guo Yong Li Wei Zhao Meixiao Zhan Yucai Wang Ligong Lu | 2021 | Nano Research2021，14，11： | 1 |
| 16 | Omicron-specific mRNA vaccine elicits potent immune responses in mice, hamsters, and nonhuman primates显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has infected more than 400 million people globally,resulting in millions of deaths.1 As the virus evolves,a new variant,designated as“Omicron”by the World Health Organization(SARS-CoV-2 variant B.1.1.529),has led to a massive resurgence of COVID-19 cases in many regions.2 The Omicron variant is highly contagious,with a significant transmission advantage compared to prior variants.Currently,Omicron represents~95%of the new infections in the US,Europe,Brazil,South Africa,and elsewhere.3–5 The remarkable transmissibility of Omicron is likely attributable to its highly mutated spike(S)gene(more than 30 mutations),which promotes S protein binding to the cellular receptor angiotensin-converting enzyme 2(ACE2)and allows it to escape from both humoral and cellular immunity elicited by prior infection or vaccination.6,7 Several studies show that the Omicron variant significantly affects the protection conferred by previous vaccinations including mRNA vaccines of mRNA-1273(Moderna)and BNT162b2(Pfizer BioNTech)。 | Yi Wu Yanqiong Shen Namei Wu Xinghai Zhang Shaohong Chen Chang Yang Junhui Zhou Yan Wu Da Chen Li Wang Chao Wang Huajun Zhang Ninuo Xia Sandra Chiu Yucai Wang | 2022 | Cell Research2022，32，10： | 0 |
| 17 | POLG Mutations Are Probably Rare in the Han Chinese Population显示文摘Objective Mutations in polymerase gamma gene(POLG)are believed to be an important cause of early and juvenile onset of non-syndromic intractable epilepsy.The aim of this study is to investigate the incidence/prevalence of POLG pathogenic variants in epilespy patients of Han population through sequencing it.Methods Han Chinese patients with seizures prior valproic acid(VPA)exposure at Shanghai Children\Hospital were collected from 2015 to 2019.The clinical diagnosis was based on the 2014 Consensus Statement of Epilepsy by the International League against Epilepsy(ILAE).Blood sampling were performed before VPA treatment.The POLG gene DNA was sequenced by either the first or the next generation sequencing(NGS).The POLG variant burden was illustrated.Liver functions were tested to describe whether they experienced VPA toxicity.Results Ibtally 216 Han Chinese patients were included,aged from 1 month to IS years old,102 were male and 114 were female.The onset age was 1 month old to 13 years old,and the epilepsy course ranged from 2 weeks to about 3 years.VPA treatment was delivered for the generalized or intractable partial seizures at standard dosage.No patient experienced hepatic toxicity following VPA exposure.DNA sequencing data showed no patient had either a homozygous mutation or compound heterozygous mutation of POLG.Single heterozygous mutations of c.1150G>T and p.D384Y were found in 2 patients,and single heterozygous mutation of c.l56_158dupGCA was found in 1 patient.None of these variants showed clinical significance.Conclusions Functional modifying POLG homozygous mutations and compound heterozygous mutations were not detected and VPA toxicity was not seen in the current study.POLG mutation frequency might be rare in Han Chinese,and standard VPA.therapeutic dosage might be safe for Han Chinese patients. | Kunfang Yang Linyi Meng Yuanfeng Zhang Yongchen Yang Hongyi Cheng Zhihu Jiang Hong Zhang Yucai Chen | 2020 | Chinese Medical Sciences Journal2020，35，4： | 0 |
| 18 | Rhizoma Chuanxiong regulates vascular endothelial growth factor production in hypoxic human umbilical vein endothelial cells in vitro and in peri-infarct rat brain tissue显示文摘BACKGROUND:Vascular endothelial growth factor(VEGF) acts as'molecular bridge'following ischemic stroke to improve and restore blood supply and reduce infarction volume.Clinical studies have demonstrated the efficacy of Rhizoma Chuanxiong(Chuanxiong) in the treatment of ischemic cerebrovascular diseases.However,whether it promotes endogenous VEGF expression in ischemic stroke remains unknown. OBJECTIVE:To investigate the influence of Rhizoma Chuanxiong on VEGF production in vitro cultured human umbilical vein endothelial cells and on VEGF expression in ischemic cerebral tissues to explore its role in angiogenesis. DESIGN,TIME AND SETTING:In vitro basic comparison of traditional Chinese drug-containing serum pharmacology;in vivo randomized,controlled,animal experiment.This study was performed at the Medical Laboratory of West China Hospital,Sichuan University between December 2002 and April 2004. MATERIALS:Two Chinese rabbits were selected.One was intragastrically perfused with 5.8 g/kg Rhizoma Chuanxiong extract twice per day for three consecutive days to prepare Rhizoma Chuanxiong extract-containing serum.The remaining rabbit was intragastrically perfused with the same volume of normal saline twice per day for three consecutive days.Rhizoma Chuanxiong extract was provided by Beijing Traditional Chinese Medicine Research Institute,predominantly composed of ligustrazine,ligustilide,and ferulic acid.ChemiKine^(TM) human VEGF Kit was purchased from Chemicon,USA;mouse anti-VEGF monoclonal antibody and biotin-goat anti-mouse IgG were purchased from Santa Cruz Biotechnology.Inc.,USA. METHODS:(1) In vitro experiment:in vitro cultured human umbilical vein endothelial cells were separately incubated in rabbit serum with 10%Rhizoma Chuanxiong extract,normal medium without rabbit serum,and rabbit serum without Rhizoma Chuanxiong extract(blank control).In addition,cells from the three groups were incubated under normoxia(5%CO_2,95%air) and hypoxia (1%O_2,5%CO_2,94%N_2),respectively,for 24 hours.(2) In vivo experiment:a total of 4/44 Sprague Dawley rats were selected for the sham-operated group(no occlusion),and the remaining rats were used to establish a cerebral ischemia/reperfusion model by suture occlusion.32 animals with ischemia/reperfusion injury were randomly divided into treatment and model groups,with 16 rats in each group.Both groups were intraperitoneally infused with 0.58 g/kg Rhizoma Chuanxiong extract and normal saline two hours following reperfusion.The sham-operated group was administrated normal saline.Animals were treated with saline or Chuanxiong extracts(0.58 g/kg) twice per day for three consecutive days. MAIN OUTCOME MEASURES:In vitro experiment:VEGF concentration was detected in each group by enzyme-linked immunosorbent assay.In vivo experiment:behavioral alterations of rats were evaluated by neurological function scale;infarct volume was assessed by hematoxylin-eosin staining;VEGF protein expression in the infarct regions was determined by immunohistochemistry. RESULTS:(1) VEGF levels were similar between the three groups under normoxic condition(P>0.05);while hypoxia induced VEGF production(P<0.01).VEGF levels in the drug-containing serum group were particularly higher compared with the other groups(P<0.01).(2) Compared with normal saline treatment,Rhizoma Chuanxiong extract significantly improved the neurological scale and reduced cerebral infarct volumes(P<0.05).The percent of VEGF-positive cells was significantly greater than the model group(P<0.05).The sham-operated group exhibited normal neurological function,with no infarct focus. CONCLUSION:Rhizoma Chuanxiong extract-containing rabbit serum effectively promoted in vitro cultured VEGF production under hypoxia.Rhizoma Chuanxiong extract upregulated VEGF expression in the infarct region,improved neurological function,and reduced infarct size. | Muke Zhou Mi Yang Ning Chen Yucai Wang Jian Guo Xue Yang Zhijian Zhang Dong Zhou Li He | 2009 | Neural Regeneration Research2009，4，5： | 0 |
| 19 | Programmed sequential cutting endows Cas9 versatile base substitution capability in plants显示文摘Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants. | Wei Yang Wei Qi Yucai Li Jiyao Wang Yanmin Luo Dehui Ding Sudong Mo Bo Chen Yu Lu Huarong Li Linjian Jiang | 2021 | Science China(Life Sciences)2021，64，6： | 0 |
| 20 | The application of artificial intelligence in the management of sepsis显示文摘Sepsis is a complex and heterogeneous syndrome that remains a serious challenge to healthcare worldwide.Patients afflicted by severe sepsis or septic shock are customarily placed under intensive care unit(ICU)supervision,where a multitude of apparatus is poised to produce high-granularity data.This reservoir of high-quality data forms the cornerstone for the integration of AI into clinical practice.However,existing reviews currently lack the inclusion of the latest advancements.This review examines the evolving integration of artificial intelligence(AI)in sepsis management.Applications of artificial intelligence include early detection,subtyping analysis,precise treatment and prognosis assessment.AI-driven early warning systems provide enhanced recognition and intervention capabilities,while profiling analyzes elucidate distinct sepsis manifestations for targeted therapy.Precision medicine harnesses the potential of artificial intelligence for pathogen identification,antibiotic selection,and fluid optimization.In conclusion,the seamless amalgamation of artificial intelligence into the domain of sepsis management heralds a transformative shift,ushering in novel prospects to elevate diagnostic precision,therapeutic efficacy,and prognostic acumen.As AI technologies develop,their impact on shaping the future of sepsis care warrants ongoing research and thoughtful implementation. | Jie Yang Sicheng Hao Jiajie Huang Tianqi Chen Ruoqi Liu Ping Zhang Mengling Feng Yang He Wei Xiao Yucai Hong Zhongheng Zhang | 2023 | Medical Review2023，3，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员