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| 1 | OsMYB103 is required for rice anther development by regulating tapetum development and exine formation显示文摘The Arabidopsis AtMYB103 gene is required for anther development,but whether the homologous gene in rice has the same role is unclear.Sequence analysis indicated that the rice OsMYB103 gene shares a high sequence similarity with AtMYB103.Therefore,we investigated the functional role of OsMYB103 in anther development using an RNAi approach.The OsMYB103 RNA transcript was expressed most abundantly in flowers,specifically in the tapetum,premeiotic pollen mother cells,and meiotic PMCs.OsMYB103-RNAi transgenic lines grew normally during their vegetative phase but displayed reduced male fertility,a phenotype that was associated with downregulated OsMYB103 transcript levels.Expression of OsMS2,an ortholog of the Arabidopsis AtMS2 gene,was also dramatically reduced in the transgenic plants.Knockdown of OsMYB103 led to defects in tapetum development,and most of the microspores in mature anthers lacked exines.Moreover,OsMYB103 could partially rescue the male sterility phenotype of an AtMYB103 knockout mutant ms188.Taken together,these results indicate that OsMYB103 does have an important role in rice tapetum and microspore development. | ZHANG Sen FANG ZiJun ZHU Jun GAO JuFang YANG ZhongNan | 2010 | Chinese Science Bulletin2010，55，29： | 12 |
| 2 | Medical expenditures for colorectal cancer diagnosis and treatment: A 10-year high-level-hospital-based multicenter retrospective survey in China, 2002-2011显示文摘Objective: Colorectal cancer(CRC) causes a substantial burden of disease in China and the evidence of economic burden triggered is fundamental for priority setting. The aim of this survey was to quantify medical expenditures and the time trends for CRC diagnosis and treatment in China.Methods: From 2012 to 2014, a hospital-based multicenter retrospective survey was conducted in 13 provinces across China. For each eligible CRC patient diagnosed from 2002 to 2011, clinical information and expenditure data were extracted using a uniform questionnaire. All expenditure data were reported in Chinese Yuan(CNY)using 2011 values.Results: Of the 14,536 CRC patients included, the average age at diagnosis was 58.2 years and 15.8% were stageI cases. The average medical expenditure per patient was estimated at 37,902 CNY [95 % confidence interval(95%CI): 37,282-38,522], and the annual average increase rate was 9.2% from 2002 to 2011(P for trend <0.001), with a cumulative increase of 2.4 times(from 23,275 CNY to 56,010 CNY). The expenditure per patient in stages Ⅰ, Ⅱ, Ⅲ and Ⅳ were 31,698 CNY, 37,067 CNY, 38,918 CNY and 42,614 CNY, respectively(P<0.001). Expenditure significantly differed within various subgroups. Expenses for drugs contributed the largest proportion(52.6%).Conclusions: These conservative estimates illustrated that medical expenditures for CRC diagnosis and treatment in tertiary hospitals in China were substantial and increased rapidly over the 10 years, with drugs continually being the main expense by 2011. Relatively, medical expenditures are lower for CRC in the earlier stages. These findings will facilitate the economic evaluation of CRC prevention and control in China. | Jufang Shi Guoxiang Liu Hong Wang Ayan Mao Chengcheng Liu Lanwei Guo Huiyao Huang Jiansong Ren Xianzhen Liao Yana Bai Xiaojie Sun Xinyu Zhu Jialin Wang Bingbing Song Jinyi Zhou Lin Zhu Haike Lei Yuqin Liu Yunyong Liu Lingbin Du Yutong He Kai Zhang Ni Li Wanqing Chen Min Dai Jie He | 2019 | Chinese Journal of Cancer Research2019，31，5： | 8 |
| 3 | No expenditure difference among patients with liver cancer at stageⅠ-ⅣV:Findings from a multicenter cross-sectional study in China显示文摘Objective:The number of liver cancer patients in China accounts for more than half of the world.However,China currently lacks national,multicenter economic burden data,and meanwhile,measuring the differences among different subgroups will be informative to formulate corresponding policies in liver cancer control.Thus,the aim of the study was to measure the economic burden of liver cancer by various subgroups.Methods:A hospital-based,multicenter and cross-sectional survey was conducted during 2012・2014,covering 39 hospitals and 21 project sites in 13 provinces across China.The questionnaire covers clinical information,sociology,expenditure,and related variables.All expenditure data were reported in Chinese Yuan(CNY)using 2014 values.Results:A total of 2,223 liver cancer patients were enrolled,of whom 59.61%were late-stage cases(III-IV),and 53.8%were hepatocellular carcinoma.The average total expenditure per liver cancer patient was estimated as 53,220 CNY,including 48,612 CNY of medical expenditures(91.3%)and 4,608 CNY of non-medical expenditures(8.7%).The average total expenditures in stage I,H,m and stage IV were 52,817 CNY,50,877 CNY,50,678 CNY and 54,089 CNY(P>0.05),respectively.Non-medical expenditures including additional meals,additional nutrition care,transportation,accommodation and hired informal nursing were 1,453 CNY,839 CNY,946 CNY,679 CNY and 200 CNY,respectively.The one-year out-of-pocket expenditure of a newly diagnosed patient was 24,953 CNY,and 77.2%of the patients suffered an unmanageable financial burden.Multivariate analysis showed that overall expenditure differed in almost all subgroups(P<0.05),except for sex,clinical stage,and pathologic type.Conclusions:There was no difference in treatment expenditure for liver cancer patients at different clinical stages,which suggests that maintaining efforts on treatment efficacy improvement is important but not enough.To fiirtherly reduce the overall economic burden from liver cancer,more effort should be given to primary and secondary prevention strategies. | Haike Lei Lin Lei Jufang Shi Yongzhong Wu Ling Liang Huiyao Huang Mei He Fangzhou Bai Maomao Cao Hui Qiu Yuting Wang Chengcheng Liu Jia Du Hong Wang Yan Zhang Mengdi Cao Ji Peng Ni Li Chunfeng Qu Min Dai Wanqing Chen Jie He | 2020 | Chinese Journal of Cancer Research2020，32，4： | 7 |
| 4 | Short-term environmental enrichment exposure induces proliferation and maturation of doublecortin-positive cells in the prefrontal cortex显示文摘Previous studies have demonstrated that doublecortin-positive immature neurons exist predominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine(a marker of cell proliferation), c-Fos(a marker of cell viability) and NeuN(a marker of mature neurons). Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs. | Chunling Fan Mengqi Zhang Lei Shang Ngobe Akume Cynthia Zhi Li Zhenyu Yang Dan Chen Jufang Huang Kun Xiong | 2014 | Neural Regeneration Research2014，9，3： | 4 |
| 5 | Synthesis of 3,5-Dichloro-4-(1,1,2,2-tetrafluoroethoxy)phenyl Containing 1,2,3-Thiadiazole Derivatives via Ugi Reaction and Their Biological Activities显示文摘包含 4-methyl-1,2,3-thiadiazole 衍生物的一系列新奇 3,5-dichloro-4-(1,1,2,2-tetrafluoroethoxy ) 苯基经由 Ugi 反应被设计并且综合。他们的结构被红外, 1H NMR, 13C NMR 和高分辨率的集体光谱学证实。初步的生物鉴定结果显示一些标题混合物在 50 g/mL 举办了好杀真菌剂活动;大多数混合物在 500 g/mL 和 100 g/mL 对烟草马赛克病毒介绍了直接抑制活动,好 inactivation 和药品活动的某个度;一些混合物对 Plutella xylostella L 显示出好 larvicidal 活动。在对在 2 g/mL 的一种蚊虫 pipiens pallens 的 200 g/mL 和优秀 larvicidal 活动。 | Wang Shouxin Wang Huan Fan Zhijin Fu Yifeng Mi Na Zhang Jufang Zhang Zhengcai Belskaya Nataliya P. Bakulev Vasiliy A. | 2011 | Chinese Journal of Chemistry2011，29，2： | 4 |
| 6 | Improved esophageal squamous cell carcinoma screening effectiveness by risk-stratified endoscopic screening:evidence from high-risk areas in China显示文摘Background:Risk-stratified endoscopic screening(RSES),which offers endoscopy to those with a high risk of esophageal cancer,has the potential to increase effectiveness and reduce endoscopic demands compared with the universal screening strategy(i.e.,endoscopic screening for all targets without risk prediction).Evidence of RSES in high-risk areas of China is limited.This study aimed to estimate whether RSES based on a 22-score esophageal squamous cell carcinoma(ESCC)risk prediction model could optimize the universal endoscopic screening strategy for ESCC screening in high-risk areas of China.Methods:Eight epidemiological variables in the ESCC risk prediction model were collected retrospectively from 26,618 individuals aged 40-69 from three high-risk areas of China who underwent endoscopic screening betweenMay 2015 and July 2017.The model’s performance was estimated using the area under the curve(AUC).Participants were categorized into a high-risk group and a low-risk group with a cutoff score having sensitivities of both ESCC and severe dysplasia and above(SDA)at more than 90.0%.Results:The ESCC risk prediction model had an AUC of 0.80(95% confidence interval:0.75-0.84)in this external population.We found that a score of 8(ranging from 0 to 22)had a sensitivity of 94.2% for ESCC and 92.5% for SDA.The RSES strategy using this threshold score would allow 50.6% of endoscopies to be avoided and save approximately US$0.59 million compared to universal endoscopic screening among 26,618 participants.In addition,a higher prevalence of SDA(1.7%vs.0.9%),a lower number need to screen(60 vs.111),and a lower average cost per detected SDA(US$3.22 thousand vs.US$5.45 thousand)could have been obtained by the RSES strategy.Conclusions:The RSES strategy based on individual risk has the potential to optimize the universal endoscopic screening strategy in ESCC high-risk areas of China. | He Li Chao Ding Hongmei Zeng Rongshou Zheng Maomao Cao Jiansong Ren Jufang Shi Dianqin Sun Siyi He Zhixun Yang Yiwen Yu Zhe Zhang Xibin Sun Guizhou Guo Guohui Song Wenqiang Wei Wanqing Chen Jie He | 2021 | Cancer Communications2021，41，8： | 3 |
| 7 | Signal interaction between nitric oxide and hydrogen peroxide in heat shock-induced hypericin production of Hypericum perforatum suspension cells显示文摘Heat shock(HS, 40℃, 10 min) induces hypericin production, nitric oxide(NO) generation, and hydrogen peroxide(H2O2) accumulation of Hypericum perforatum suspension cells.Catalase(CAT) and NO spe-cific scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide(cPTIO) suppress not only the HS-induced H2O2 generation and NO burst, but also the HS-triggered hypericin produc-tion.Hypericin contents of the cells treated with both NO and H2O2 are significantly higher than those of the cells treated with NO alone, although H2O2 per se has no effects on hypericin production of the cells, which suggests the synergistic action between H2O2 and NO on hypericin production.NO treatment enhances H2O2 levels of H.perforatum cells, while external application of H2O2 induces NO generation of cells.Thus, the results reveal a mutually amplifying action between H2O2 and NO in H.perforatum cells.CAT treatment inhibits both HS-induced H2O2 accumulation and NO generation, while cPTIO can also suppress H2O2 levels of the heat shocked cells.The results imply that H2O2 and NO may enhance each other's levels by their mutually amplifying action in the heat shocked cells.Membrane NAD(P)H oxidase inhibitor diphenylene iodonium(DPI) and nitric oxide synthase(NOS) inhibitor S,S′-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea(PBITU) not only inhibit the mutually amplifying action between H2O2 and NO but also abolish the synergistic effects of H2O2 and NO on hypericin production, showing that the synergism of H2O2 and NO on secondary metabolite biosynthesis might be dependent on their mutual amplification.Taken together, data of the present work demonstrate that both H2O2 and NO are essential for HS-induced hypericin production of H.perforatum suspension cells.Furthermore, the results reveal a special interaction between the two signal molecules in mediating HS-triggered secondary metabolite biosynthesis of the cells. | XU MaoJun, DONG JuFang & ZHANG XinBo Institute of Natural Resource and Environmental Sciences, Zhejiang Gongshang University, Hangzhou 310035, China | 2008 | Science China(Life Sciences)2008，51，8： | 3 |
| 8 | Modeling of protein refolding from inclusion bodies显示文摘在 Escherichia coli 的外国蛋白质的 Overexpression 经常导致包括身体(IB ) 的形成,它在 recombinant 蛋白质和他们的应用的准备成为主要瓶颈。在现在的学习,从 IB 的 36 蛋白质是用一个简单 refolding 方法的 refolded。这些蛋白质的 Refolding 收益被定义为在冲淡进 refolding 缓冲区前在样品在使中毒的蛋白质的数量跟随冲淡 refolding 的可溶的蛋白质的百分比。而且,一个数学模型被推出在蛋白质 refolding 评估生物化学的性质的角色。自从一个单位的增长在 refolding 收益导致了 14.83% 的减少,我们的结果显示在试验性的条件下面,蛋白质的等电位的点可能主要正在贡献蛋白质 refolding 的高功效。另外的重要调停人是蛋白质的部件第二等的结构和分子的重量(R2 = 0.98, P = 0.000, F-test ) 。有在蛋白质 refolding 的低效率的六蛋白质拥有了相对低的等电位的点。而且,从 IB 的六另外的蛋白质的 refolding 收益被 refolding 在一样的条件下面预言并且进一步验证了蛋白质。因此,这里开发的蛋白质 refolding 的模型能被用来通过一个简单方法从 IB 预言蛋白质的 refolding 收益。我们的学习将是暗示的根据他们的内在的性质从 IB 为蛋白质 refolding 优化方法。 | Ting Zhang Xiaojing Xu Liang Shen Yanye Feng Zhong Yang Yaling Shen Jufang Wang Weirong Jin Xiaoning Wang | 2009 | Acta Biochimica et Biophysica Sinica2009，41，12： | 2 |
| 9 | The automatic control of the pumped start-up of the pumped-storage unit显示文摘 | Yang Wendao Zhang Yanyu Pan Jufang | 2015 | Hydropower and Pumped Storage2015，1，2： | 1 |
| 10 | An effective method of DNA extraction for bioleaching bacteria from acid mine drainage显示文摘 | Leping Zeng Jufang Huang Yanfei Zhang Guanzhou Qiu Jianbin Tong Dan Chen Jin Zhou Xuegang Luo | 2008 | Applied Microbiology and Biotechnology2008，，5： | 1 |
| 11 | Modulatory effects of one polysaccharide from Acanthopanax senticosus in alloxan-induced diabetic mice显示文摘 | Jianfang Fu Jufang Fu Yang Liu Ruiyu Li Bin Gao Nanyan Zhang Boliang Wang Yizhan Cao Kaoshan Guo Yanyang Tu | 2011 | Carbohydrate Polymers2011，，3： | 1 |
| 12 | Cold-induced endogenous nitric oxide generation plays a role in chilling tolerance of loquat fruit during postharvest storage显示文摘 | Maojun Xu Jufang Dong Ming Zhang Xiangbin Xu Lina Sun | 2011 | Postharvest Biology and Technology2011，，： | 1 |
| 13 | Cold-induced endogenous nitric oxide generation plays a role in chilling tolerance of loquat fruit during postharvest storage显示文摘 | Xu Maojun Dong Jufang Zhang Ming | | 0，，： | 1 |
| 14 | First identification of xanthone sulfonamides as potent acyl-CoA:cholesterol acyltransferase (ACAT) inhibitors显示文摘 | Honggang Hu Hongli Liao Jun Zhang Weifeng Wu Jufang Yan Yonghong Yan Qingjie Zhao Yan Zou Xiaoyun Chai Shichong Yu Qiuye Wu | 2010 | Bioorganic & Medicinal Chemistry Letters2010，，10： | 1 |
| 15 | Ozone induces flavonol production of Ginkgo biloba cells dependently on nitrate reductase-mediated nitric oxide signaling显示文摘 | Maojun Xu Yun Zhu Jufang Dong Haihong Jin Lina Sun Zhian Wang Zhonghua Lu Ming Zhang Dan Lu | 2011 | Environmental and Experimental Botany2011，，： | 1 |
| 16 | Cold-induced endogenous nitric oxide generation plays a role in chilling tolerance of loquat fruit during postharvest storage显示文摘 | XU Maojun DONG Jufang ZHANG Ming | 2012 | Postharvest Biology and Technology2012，65，： | 1 |
| 17 | Nitric oxide fumigation stimulates flavonoid and phenolic accumulation and enhances antioxidant activity of mushroom显示文摘 | DONG Jufang ZHANG Ming LU Li | 2012 | Food Chemistry2012，135，3： | 1 |
| 18 | Melatonin alleviates pyroptosis of retinal neurons following acute intraocular hypertension显示文摘Glaucoma is a multifactorial optic neuropathy progressive characterized by structural loss of retinal ganglion cells(RGCs)and irreversible loss of vision.High intraocular pressure(HIOP)is a high risk factor for glaucoma.It has been reported that the manners of RGCs'loss are in-depth explored after acute HIOP injury,such as,apoptosis,autophagy and necrosis.However,pyroptosis,a novel type of pro-inflammatory cell programmed necrosis,rarely reported after HIOP injury.Researches also showed that melatonin possessing substantial anti-inflammatory properties.However,whether melatonin could alleviate retinal neurons death,especially pyroptosis,by HIOP injury is unclear.This study explored pyroptosis of retinal neurons and the effects of melatonin preventing retinal neurons form pyroptosis after acute HIOP injury.Establish acute HIOP model in rat by increasing the IOP and then reperfusion. | Zhang Yu Huang Yanxia Guo Limin Zhang Yun Zhao Mingxuan Xue Fuyao Tan Cheng Huang Jufang Chen Dan | 2021 | 解剖学杂志2021，44，S01： | 1 |
| 19 | Generalized hetero- chronous synchronization in coupled time-delayed chaotic systems 显示文摘 | Zhang Xiaoming Chen Jufang Peng Jianhua | 2014 | Nonlinear Dynamics2014，78，4： | 1 |
| 20 | Cholecystokinin from the entorhinal cortex enables neural plasticity in the auditory cortex显示文摘有中间的时间的脑叶的损坏的病人处于良好的竟技状态新说明记忆显示出赤字,但是能仍然记起更旧的记忆,建议中间的时间的脑叶为把记忆编码为 neocortex 是必要的。这里,我们发现在 perirhinal 和 entorhinal cortices 的外皮的设计神经原主要是为 cholecystokinin (CCK ) 的 immunopositive。在 anesthetized 老鼠的听觉的外皮的 CCK 的本地注入导致了使外皮的神经原能加强的塑料变化他们的回答或到开始回答与一个音调被配对到听觉的刺激,那要用体力地触发了行动潜力。CCK 注入也使听觉的神经原能开始回答与噪音爆炸被配对到轻刺激。在 vivo,在听觉的外皮的细胞内部的记录证明 synaptic 力量面对 CCK 在 presynaptic 和 postsynaptic 活动的二配对以后被加强。CCK 在听觉的外皮的 B 对手阻止了一个 visuo 听觉的协会的形成在唤醒老鼠。最后, entorhinal 外皮的激活加强了在听觉的外皮的 neuronal 回答,它被 CCK B 对手。一起,这些调查结果建议中间的时间的脑叶在 entorhinal 外皮经由 CCK 积极的外皮的设计神经原影响 neocortical 粘性。 | Xiao Li | 2014 | Cell Research2014，24，3： | 1 |
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