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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Temperature variation of a thermionic cathode during electron emission显示文摘It is necessary to know the actual temperature of a thermionic cathode that works as the electron source in a microwave tube. It has been found that the temperature of the cathode drops markedly during the thermionic emission. For example, the temperature could fall by about 30oC under a current density of 2.92 A/cm2. Using the molecular thermodynamics, the dependence of the cathode temperature on the emission current density has been obtained. It has been theoretically pointed out that several factors, such as heating model and temperature coefficient of resis-tance of heater, can influence the cathode temperature. These theoretical conclu-sions were supported by the experimental results. | LIU YanWen TIAN Hong HAN Yong XU ZhenYing MENG MingFeng ZHANG HongLai | 2008 | Science China(Technological Sciences)2008，51，9： | 22 |
| 4 | Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats显示文摘OBJECTIVE: To investigate the anti-infl ammatory, anti-oxidative stress, and adipokine-ameliorating effects of Liuwei Dihuang(LWDH), a traditional Chinese herbal formula, in obese rats. METHODS: After 2 weeks of acclimation with free access to regular rodent chow and water, obese-prone-caesarean-derived(OP-CD) rats were fed a modified AIN-93 G diet containing 60% energy from fat. Treatment was performed twice daily by gavage feeding with 500, 1 500, or 3 500 mg/kg body weight LWDH suspended in water(n=12 rats per group). Twelve obese-resistant-CD(OR-CD) rats were fed the atherogenic diet and gavaged with water, and served as the normal control. Blood biomarkers of inflammation, oxidative stress and adiponectin were measured post-sacrifi ce and used to determine the treatment effect of LWDH and assess the suitability of OR/OP-CD rats for studying these parameters. RESULTS: After 9 weeks of treatment, LWDH lowered serum C-reactive protein(CRP) and tumour necrosis factor-α(TNF-α) levels. Serum interleukin-6(IL-6) levels showed a tendency towards reduction, but were not signifi cantly different from the OP-CD control. Liver superoxide dismutase(SOD) activity was increased in response to all three doses of LWDH, while the levels of reduced(GSH) and oxidized glutathione(GSSG) and thiobarbituric acid reactive substances(TBARS) were unchanged. Serum adiponectin levels were increased in response to oral administration of LWDH at the dose of either 500 or 1 500 mg/kg body weight. In addition, comparisons between OR-CD and OP-CD rats revealed differential, and for some biomarkers, conflicting characteristics of high-fat diet-fed OP-CD rats in reference to obese human subjects in terms of infl ammatory and oxidative stress biomarkers and circulating adiponectin levels. CONCLUSION: The results show, for the fi rst time, the anti-infl ammatory, anti-oxidative stress and adiponectin-ameliorating effects of LWDH in obese rats. The suitability of the OR/OP-CD rat model as a research tool to study infl ammation, oxidative stress, and adipokine production requires further investigation. | Benjamin Perry Junzeng Zhang Tarek Saleh Yanwen Wang | 2014 | Journal of Integrative Medicine2014，12，5： | 14 |
| 5 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 6 | Carbon-nitrogen/graphene composite as metal-free electrocatalyst for the oxygen reduction reaction显示文摘Sheet-like carbon-nitrogen (CNx)/graphene composites with a high content of nitrogen (x≤0.15) was prepared by the carbonization of polypyrrole (PPy)/reduced-graphene-oxide (rGO) composite at 600-800°C. We used rGO instead of graphene oxide (GO) sheets as a template and a substrate to immobilize PPy since the PPy/GO composite agglomerates easily because of the dehydration of excess oxygen-containing groups on the GO sheets during the drying process. The dried PPy/rGO intermediate and its derived CNx/graphene products retain their high dispersion and loose-powder features. The as-prepared CNx/graphene composites have a total nitrogen content of about 10 at% and their nitrogen state is mainly of pyridinic and graphitic type. CNx/graphene composites exhibit excellent performance for the oxygen reduction reaction (ORR) in terms of electrocatalytic activity, stability and immunity towards methanol crossover and CO poisoning, suggesting their potential as metal-free electrocatalysts for the ORR. | MA YanWen ZHANG LingRong LIJuanJuan NI HaiTao LI Meng ZHANG JinLei FENG XiaoMiao FAN QuLi HU Zheng HUANG Wei | 2011 | Chinese Science Bulletin2011，56，33： | 11 |
| 7 | Steady performance of a zero valent iron packed anaerobic reactor for azo dye wastewater treatment under variable influent quality显示文摘Zero valent iron(ZVI) is expected to help create an enhanced anaerobic environment that might improve the performance of anaerobic treatment.Based on this idea,a novel ZVI packed upflow anaerobic sludge blanket(ZVI-UASB) reactor was developed to treat azo dye wastewater with variable influent quality.The results showed that the reactor was less influenced by increases of Reactive Brilliant Red X-3B concentration from 50 to 1000 mg/L and chemical oxygen demand(COD) from 1000 to 7000 mg/L in the feed than a reference UASB reactor without the ZVI.The ZVI decreased oxidation-reduction potential in the reactor by about 80 mV.Iron ion dissolution from the ZVI could buffer acidity in the reactor,the amount of which was related to the COD concentration.Fluorescence in situ hybridization test showed the abundance of methanogens in the sludge of the ZVI-UASB reactor was significantly greater than that of the reference one.Denaturing gradient gel electrophoresis showed that the ZVI increased the diversity of microbial strains responsible for high efficiency. | Yaobin Zhang Yiwen Liu Yanwen Jing Zhiqiang Zhao Xie Quan | 2012 | Journal of Environmental Sciences2012，24，4： | 9 |
| 8 | Receptivity to free-stream disturbance waves for hypersonic flow over a blunt cone显示文摘A high-order shock-fitting finite difference scheme is studied and used to do direc-tion numerical simulation (DNS) of hypersonic unsteady flow over a blunt cone with fast acoustic waves in the free stream, and the receptivity problem in the blunt cone hypersonic boundary layers is studied. The results show that the acoustic waves are the strongest disturbance in the blunt cone hypersonic boundary layers. The wave modes of disturbance in the blunt cone boundary layers are first, second, and third modes which are generated and propagated downstream along the wall. The results also show that as the frequency decreases, the amplitudes of wave modes of disturbance increase, but there is a critical value. When frequency is over the critial value, the amplitudes decrease. Because of the discontinuity of curvature along the blunt cone body, the maximum amplitudes as a function of frequencies are not monotone. | ZHANG YuDong FU DeXun MA YanWen LI XinLiang | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，11： | 8 |
| 9 | Mobility and sulfidization of heavy metals in sediments of a shallow eutrophic lake, Lake Taihu, China显示文摘The technique of DGT(diffusive gradients in thin films) using three diffusive gel thicknesses was applied to estimate the mobility and bioavailability of heavy metals in sediments and porewater of Lake Taihu, China. The DGT results showed significantly positive correlations between Co, Pb, Cd and Mn, and Ni and Fe concentrations in porewater. Cu and Zn showed a significantly negative correlation with Mn, due to Cu combination with carbonates and Zn derived from agricultural pollution, respectively. The rank order of average concentrations of Co, Ni and Cd at each station was DGT1.92>DGT0.78> DGT0.39, suggesting stronger resupply from sediments to porewater when using thicker diffusive gels. Comparing centrifugation and DGT measurements, Co, Ni and Cd are highly labile; Mn and Fe are moderately labile; and Cu, Zn and Pb are slightly labile. The variations of AVS concentrations in sediment cores indicate that metal sulfides in deeper layers are easily diffused into surface sediments. | Shouliang Huo Jingtian Zhang Kevin M.Yeager Beidou Xi Yanwen Qin Zhuoshi He Fengchang Wu | 2015 | Journal of Environmental Sciences2015，27，5： | 8 |
| 10 | Discussion on Evolution of Land-use Planning in China | CAI Yumei,Land Planning Department,Land Investigation and Planning Institute of China,Beijing,P.R.China. ZHANG Wenxin,Geography and RS Science School,Beijing Normal University,Beijing,P.R.China. ZHAO Yanwen,Resource and Environment Science College,Nanjing Agricultural University,Nanjing,P.R.China. SHI Ke | 2009 | China City Planning Review2009，18，1： | 4 |
| 11 | Wafer-scale arrayed p-n junctions based on few-layer epitaxial GaTe显示文摘二维(2D ) 材料吸引了实质的注意在电子并且有灵活、透明、高度悦耳的优异优点的 optoelectronic 应用。当时,无差距的 graphene 展出极端宽带、快的 photoresponse 半导体的瞬间 2 和门高展出的 2D 敏感和悦耳的 responsivity 到可见的光。然而,设备收益和重覆性打电话让进一步的改进完成大规模一致性。这里,我们与 28.4 厘米 2/(V0 的高洞活动性报导晶片规模门的 layer-by-layer | Xiang Yuan Lei Tang Peng Wang Zhigang Chen Yichao Zou Xiaofeng Su Cheng Zhang Yanwen Liu Weiyi Wang Cong Liu Fansheng Chen Jin Zou Peng Zhou Weida Hu Faxian Xiu | 2015 | Nano Research2015，8，10： | 4 |
| 12 | A prospective study (SCOPE) comparing the cardiometabolic and respiratory effects of air pollution exposure on healthy and pre-diabetic individuals显示文摘Air pollution is known to be a major risk factor for cardiopulmonary disease, but this is unclear for cardiometabolic disease (e.g.diabetes). This is of considerable public health importance, given the nationwide epidemic of diabetes, accompanied by severe air pollution, in China. The evidence so far remained inadequate to answer questions of whether individuals with cardiometabolic dysfunctions are susceptible to air pollution and whether air pollution exacerbates diabetes development via certain biological pathways. In this manuscript, we summarize the results and limitations of studies exploring these two topics and elaborate our design of a prospective panel study (SCOPE) as a solution. We assessed and compared the health effect of air pollution among pre-diabetic individuals and matched healthy controls through four repeated clinical visits over 1 year. Comprehensive evaluation was made to both health endpoints and exposure. The primary biomarkers were assessed to reveal the impact on multiple biological pathways, including glycolipid metabolism and insulin resistance, endothelial function, and inflammation. Detailed chemical and size fractional components of particulate matter were measured in this study, along with the application of personal monitors.The work should increase our understanding of how air pollution affects individuals with cardiometabolic dysfunction and the underlying mechanisms. | Yanwen Wang Yiqun Han Tong Zhu Weiju Li Hongyin Zhang | 2018 | Science China(Life Sciences)2018，61，1： | 4 |
| 13 | Diagnostic value of negative enrichment and immune fluorescence in situ hybridization for intraperitoneal free cancer cells of gastric cancer显示文摘Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/17.Methods:To verify the reliability of NEimFISH,29 gastric cancer tumors,their adjacent tissues and greater omental tissues were tested.Our study then included 105 gastric cancer patients for IFCC.We defined patients as IFCC-positive if a signal was detected,regardless of the detailed cancer cell numbers.A comparison of clinicopathological features was conducted among IFCC groups.We also compared the diagnosis value and peritoneal recurrence predictive value among different detection methods.The comparison of IFCC number was also conducted among different groups.Results:A cutoff of 2.5 positive cells could distinguish all benign tissue samples and 97%of malignant tissue samples in our study.Compared to intestinal gastric cancer,patients with diffuse gastric cancer tended to have more IFCCs(6 vs.4,P=0.002).The IFCC counts were often higher in the lymphovascular invasion positive group than negative group(3 vs.1,P=0.022).All IFCC samples that were considered positive using conventional cytology were also found to be positive using NEimFISH.When compared to conventional cytology and paraffin pathology,NEimFISH had a higher IFCC positive rate(68.9%)and higher one-year peritoneal recurrence predictive value with area under the curve(AUC)of 0.922.Conclusions:Gastric cancer could be effectively diagnosed by NEimFISH.The IFCC number found using NEimFISH on CEN8/17 is closely associated with Lauren type and vascular invasion of cancer.NEimFISH is a reliable detection modality with a higher positive detection rate,higher one-year peritoneal recurrence predictive value and quantitative features for IFCC of gastric cancer. | Anqiang Wang Zhongwu Li Qian Wang Yali Bai Xin Ji Tao Fu Ke Ji Yanwen Xue Tingxu Han Xiaojiang Wu Ji Zhang Yingjie Yang Guobin Xu Zhaode Bu Jiafu Ji | 2019 | Chinese Journal of Cancer Research2019，31，6： | 3 |
| 14 | Synthesis,Characterization and Electromagnetic Studies on Nanocrystalline Nickel Zinc Ferrite by Polyacrylamide Gel显示文摘Nanocrystalline 镍锌铁酸盐粉末(Ni_xZni_(1-x ) Fe2O4,为 x=0 的 A,为 x=0.2 的 B,为 x=0.5 的 C,为 x-0.8 的 D 和为 x=1 的 E ) 被聚丙烯酰氨综合胶化方法。X 光检查衍射(XRD ) ,传播电子显微镜学(TEM ) 和波导被用来描绘作文。弄干的胶化粉末是非结晶的 XRD 结果表演,和特征尖晶石Ni_( 0.5 )Zn_( 0.5 ) Fe2O4 达到顶点在胶化为锻烧的温度是的 1 h.When 在 400 °C 被锻烧以后,出现 600 和 800 °C ,平均谷物尺寸被是 10 和 30 nm 的 TEM 识别, respectively.The Ni_xZn_( 1-x ) Fe2O4 粉末在Ni^(2+)离子的增加满足的 8.2-11.0GHz .With 的频率范围有绝缘的损失和磁性的损失,当时,绝缘的参数(epsilon')和Ni_xZn_( 1-x ) Fe2O4 粉末的渗透(mu')减少绝缘的损失( epsilon “),磁性的损失(亩”)并且思考损失增加。 | Ruiting MA Yi WANG Yanwen TIAN Chunli ZHANG Xikun LI | 2008 | Journal of Materials Science & Technology2008，24，3： | 2 |
| 15 | Operando X-ray diffraction analysis of the degradation mechanisms of a spinel LiMn2O4 cathode in different voltage windows显示文摘The understanding of reaction mechanisms of electrode materials is of significant importance for the development of advanced batteries.The LiMn2O4 cathode has a voltage plateau around 2.8 V(vs.Li^+/Li),which can provide an additional capacity for Li storage,but it suffers from a severe capacity degradation.In this study,operando X-ray diffraction is carried out to investigate the structural evolutions and degradation mechanisms of LiMn2O4 in different voltage ranges.In the range of 3.0-4.3 V(vs.Li^+/Li),the LiMn2O4 cathode exhibits a low capacity but good cycling stability with cycles up to 100 cycles and the charge/discharge processes are associated with the reversible extraction/insertion of Li^+from/into LixMn2O4(0≤x≤1).In the range of 1.4-4.4 V(vs.Li^+/Li),a capacity higher than 200 mAh/g is achieved,but it rapidly decays during the cycling.The voltage plateau around 2.8 V(vs.Li^+/Li)is related to the transformation of the cubic LiMn2O4 phase to the tetragonal Li2Mn2O4 phase,which leads to the formation of cracks as well as the performance degradation. | Fakui Luo Congcong Wei Chi Zhang Hui Gao Jiazheng Niu Wensheng Ma Zhangquan Peng Yanwen Bai Zhonghua Zhang | 2020 | Journal of Energy Chemistry2020，29，5： | 2 |
| 16 | Local-environment dependence of stacking fault energies in concentrated solid-solution alloys显示文摘Concentrated solid-solution alloys(CSAs)based on 3d transition metals have demonstrated extraordinary mechanical properties and radiation resistance associated with their low stacking fault energies(SFEs).Owing to the intrinsic disorder,SFEs in CSAs exhibit distributions depending on local atomic configurations.In this work,the distribution of SFEs in equiatomic CSAs of NiCo,NiFe,and NiCoCr are investigated based on empirical potential and first-principles calculations.We show that the calculated distribution of SFEs in chemically disordered CSAs depends on the stacking fault area using empirical potential calculations.Based on electronic structure calculations,we find that local variations of SFEs in CSAs correlate with the charge density redistribution in the stacking fault region.We further propose a bond breaking and forming model to understand and predict the SFEs in CSAs based on the local structure alone.It is shown that the perturbation induced by a stacking fault is localized in the first-nearest planes for NiCo,but extends up to the third nearest planes for NiFe and NiCoCr because of partially filled d electrons in Fe and Cr. | Shijun Zhao Yuri Osetsky G.Malcolm Stocks Yanwen Zhang | 2019 | npj Computational Materials2019，，1： | 2 |
| 17 | PM_(2.5) exposure associated with microbiota gut-brain axis:Multi-omics mechanistic implications from the BAPE study显示文摘Recent studies have shown that PM_(2.5) may activate the hypothalamus-pituitary-adrenal(HPA)axis by inducing hormonal changes,potentially explaining the increase in neurological and cardiovascular risks.In addition,an association between PM_(2.5) and gut microbiota and metabolites was established.The above evidence represents crucial parts of the gut-brain axis(GBA).In view of this evidence. | Tiantian Li Jianlong Fang Song Tang Hang Du Liang Zhao Yanwen Wang Fuchang Deng Yuanyuan Liu Yanjun Du Liangliang Cui Wanying Shi Yan Wang Jiaonan Wang Yingjian Zhang Xiaoyan Dong Ying Gao Yu Shen Li Dong Huichan Zhou Qinghua Sun Haoran Dong Xiumiao Peng Yi Zhang Meng Cao Hong Zhi Jingyang Zhou Xiaoming Shi | 2022 | The Innovation2022，3，2： | 2 |
| 18 | Synthesis,Characterization and Electromagnetic Studies on Nanocrystalline Co_(0.5)Zn_(0.5)Fe_2O_4 Synthesized by Polyacrylamide Gel显示文摘Nanocrystalline Co_(0.5)Zn_(0.5)Fe_2O_4 ferrite was synthesized by polyacrylamide gel method.The electromagnetic and microwave absorption properties of the ferrite were investigated.The results indicated that calcining temperature of the ferrite had a significant influence on the effective properties of the ferrite.When the calcining temperature was 500,600 and 700℃,the average size of particles was 10,30 and 80 nm,respectively. The dielectric loss(ε″)and magnetic loss(u″)of the ferrite was around 0.65 and 0.29 at 8.2 GHz,respectively. Microwave absorption properties of the ferrites were simultaneously influenced due to the strong correlation between reflection loss and electromagnetic parameters of the ferrite. | Ruiting MA Yanwen TIAN Haitao ZHAO Gang ZHANG Hui ZHAO | 2008 | Journal of Materials Science & Technology2008，24，4： | 2 |
| 19 | Experimental observation of the quantum Hall effect and Berry's phase in graphene显示文摘 | Zhang Yuanbo Tan Yanwen Stormer H L | | 0，，7065： | 1 |
| 20 | Experimental observation of the quantum hall effect and berry's phase in graphene 显示文摘 | ZHANG Yuanbo TAN Yanwen STORMER H | 2005 | Nature2005，438，： | 1 |
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